Multi-crosslinked protein as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-crosslinked protein preparation method, a product and application. The preparation method comprises the following steps: dissolving a collagen sample in a buffer solution, adding an antioxidant, and carrying out ultrasonic treatment to obtain a collagen solution; then adding dithio bismaleimide into the collagen solution, and stirring to form a first cross-linked protein; adding a cross-linking agent mixture into the primary cross-linked protein to form secondary cross-linked protein; and finally, adding ethylene glycol dimethacrylate and a photoinitiator into the double-crosslinked protein, uniformly stirring, and dialyzing to obtain the photoinitiator. The multi-crosslinked protein prepared by the invention has better crosslinking degree, cell survival rate and in-vitro degradation half-life period, the adopted crosslinking agent is safe and non-toxic, and the multi-crosslinked protein is suitable for skin fillers, tissue engineering scaffolds, wound dressings and other scenes with high requirements on biocompatibility.

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