Antigen impurity removal storage tank and use method thereof

Abstract

The invention relates to the technical field of antigen purification devices, and provides an antigen impurity removal storage tank which comprises a tank body, a conical baffle, a precipitation partition plate, a first discharge pipe and a second discharge pipe, the conical baffle is connected to the bottom of the tank body and encloses a liquid storage cavity with the tank body; the conical baffle obliquely extends to the inner wall surface of the tank body from the center of the tank body to the periphery from top to bottom; the precipitation partition plate is arranged in the liquid storage cavity; the precipitation partition plate is spiral; a spiral sediment accommodating channel is formed among the sediment partition plate, the conical baffle plate and the inner wall surface of the tank body; a first discharge pipe for discharging clarified antigen liquid is arranged in the center of the conical baffle, and a second discharge pipe for discharging sediment is arranged on the outer edge of the conical baffle. According to the antigen impurity removal storage tank, vaccine antigen stock solution can be primarily separated and purified, and mixing of impurity precipitates such as yolk and cell debris when the antigen is taken is effectively reduced. Meanwhile, waste caused by the fact that excessive antigens have to be discarded in order to obtain clear antigens is avoided.

Description

Technical Field

[0001] This invention relates to the field of antigen purification equipment technology, specifically to an antigen purification storage tank and its usage method. Background Technology

[0002] During the antigen production process, even after centrifugation, a certain amount of yolk or cell fragments will still remain. In addition, during low-temperature storage after inactivation with formaldehyde and other agents, some types of originally clear antigen solutions will precipitate flocculent impurities, which gradually accumulate at the bottom over time. If these impurities are not removed in time, they will affect the quality of the vaccine and must be separated.

[0003] Existing antigen storage tanks lack the capability for impurity separation and purification. This results in a significant amount of impurities contaminating the initial antigen solution during use. To avoid impacting vaccine quality, this portion of antigen with high impurity content must be discarded, effectively wasting a considerable amount of antigen.

[0004] The technical problem addressed in this case is: how to use a simple method to perform primary separation and purification of the antigen stock solution using a tank, and remove impurities. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides an antigen purification storage container. This container not only temporarily stores vaccine antigen stock solution but also automatically performs preliminary separation and purification of the antigen stock solution. Furthermore, this invention provides a method for using the antigen purification storage container, which reduces the amount of impurities such as cell debris and proteins precipitated in the vaccine antigen stock solution being used, thereby minimizing antigen waste.

[0006] The technical solution of this invention is:

[0007] An antigen removal storage tank includes a tank body, a conical baffle, a sedimentation partition, a first discharge pipe, and a second discharge pipe;

[0008] The axial direction of the tank body extends vertically;

[0009] The conical baffle is connected to the bottom of the tank and forms a liquid storage cavity with the tank; the conical baffle extends from the center of the tank downwards to the inner wall of the tank.

[0010] The sedimentation baffle is disposed in the liquid storage chamber; the sedimentation baffle is spiral-shaped and extends spirally from the center of the tank to the inner wall surface of the tank around the axis of the tank body; along the axis of the tank body, one end of the sedimentation baffle is connected to the conical baffle; the sedimentation baffle, the conical baffle and the inner wall surface of the tank body form a spiral sediment receiving channel;

[0011] in,

[0012] The first discharge pipe is located at the center of the conical baffle. The first discharge pipe is inserted into the conical baffle along the axial direction of the tank body. Its input end opening is located at the center of the conical baffle, and its output end opening is located outside the tank body. The first discharge pipe is used to discharge the clarified antigen solution in the storage chamber.

[0013] The outer edge of the conical baffle is provided with a second discharge pipe. The input end of the second discharge pipe is inserted into the sediment containing channel along the axial direction of the tank body, and its output end opening is located outside the tank body. The second discharge pipe is used to discharge the sediment in the sediment containing channel.

[0014] In practical applications, when the vaccine antigen stock solution is discharged from the first discharge pipe, the sediment containment channel can prevent the sediment from being carried up by the fluid and discharged with the clarified antigen solution. At the same time, the sediment baffle can also change the fluid motion state, prevent the generation of eddies, and reduce the risk of sediment being carried up by the fluid.

[0015] In the aforementioned antigen removal storage tank, preferably, the precipitation partition is a conical spiral structure extending from top to bottom.

[0016] In the aforementioned antigen removal storage tank, preferably, along the axial direction of the tank body, the end of the precipitation baffle away from the conical baffle is flush with the center starting end of the precipitation baffle and is flush with and connected to the inlet opening of the first discharge pipe.

[0017] In the aforementioned antigen removal storage tank, the end of the precipitation baffle located away from the center of the tank along its spiral direction is spaced apart from the inner wall of the tank to form a first gap.

[0018] In the aforementioned antigen removal storage tank, there are multiple second discharge pipes, which are arranged at intervals around the axis of the tank body.

[0019] In the aforementioned antigen removal storage tank, at least one second discharge pipe is disposed in the first gap.

[0020] In the aforementioned antigen removal storage tank, the antigen removal storage tank further includes an input pipe, which is connected to the top of the tank body and communicates with the liquid storage chamber. There are multiple input pipes, which are arranged circumferentially around the axis of the tank body.

[0021] In the aforementioned antigen removal storage tank, a manhole is provided at the top of the tank, a viewing window and a handle are provided on the outer peripheral wall of the tank, and casters are provided at the bottom of the tank.

[0022] In addition, this application also provides a method of using an antigen removal storage tank, which includes the following steps:

[0023] Step (1): Let the antigen impurity storage tank containing the vaccine antigen stock solution stand until the vaccine antigen stock solution forms a clear liquid layer and a precipitate layer, wherein cell debris or egg yolk and other impurities in the vaccine antigen stock solution fall into the precipitate receiving channel to form the precipitate layer.

[0024] Step (2): Open only the first discharge pipe to take the clarified liquid layer;

[0025] Step (3): After the liquid level of the clarified liquid layer drops below the opening of the inlet of the first discharge pipe, the clarified antigen liquid can no longer be discharged. At this time, the second discharge pipe is opened to empty the precipitate layer.

[0026] Preferably, the method further includes the following steps:

[0027] Step (4): Connect the first discharge pipe to the cleaning liquid water source, and then introduce the cleaning liquid into the storage chamber through the first discharge pipe and control the liquid inlet rate and liquid inlet volume so that the cleaning liquid flows along the sediment containing channel and is discharged from the second discharge pipe;

[0028] Step (5): After a preset time, close the second discharge pipe, then introduce cleaning fluid until the reservoir is full of cleaning fluid, then open the second discharge pipe to completely drain the cleaning fluid.

[0029] This invention has at least one of the following advantages or beneficial effects:

[0030] The conical baffle, sedimentation partition, and inner wall of the antigen removal storage tank of the present invention form a spiral sedimentation channel in the liquid storage chamber. Impurities will settle in the sedimentation channel. At the same time, the sedimentation partition in the liquid storage chamber can also avoid the risk that the eddy current formed by the outflow of the liquid will continuously carry up the sediment at the bottom and mix it into the antigen solution when the antigen solution is taken out. Under the dual effect, the content of sediment in the discharged vaccine antigen stock solution is further reduced.

[0031] The method of using the antigen purification storage tank of the present invention can effectively reduce the mixing of impurities such as egg yolk and cell debris when taking antigens, and at the same time, avoid the waste caused by having to discard too much antigen in order to obtain clear antigens. Attached Figure Description

[0032] Figure 1 This is a three-dimensional schematic diagram of the antigen removal storage tank according to Embodiment 1 of the present invention;

[0033] Figure 2 This is a front view of the antigen removal storage tank according to Embodiment 1 of the present invention;

[0034] Figure 3 This is a top view of the antigen removal storage tank according to Embodiment 1 of the present invention;

[0035] Figure 4 This is a schematic diagram showing the combination of the conical baffle and the precipitation partition in the antigen removal storage tank of Embodiment 1 of the present invention;

[0036] Figure 5 As in Embodiment 1 of the present invention Figure 4 Top view;

[0037] Figure 6 This is a schematic diagram showing the combination of the conical baffle and the sedimentation partition in the antigen removal storage tank of Embodiment 2 of the present invention;

[0038] Figure 7 This is Embodiment 2 of the present invention. Figure 6 Top view.

[0039] Explanation of reference numerals in the attached figures:

[0040] Tank body 1; viewing window 101; handle 102; caster wheel 103; conical baffle 2; sedimentation baffle 3; first discharge pipe 4; second discharge pipe 5; input pipe 6; cover 7; sediment receiving channel a; first void b. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0042] Example 1

[0043] like Figures 1-5 As shown, an embodiment of the present invention provides an antigen removal storage tank, including a tank body 1, a conical baffle 2, a sedimentation partition 3, a first discharge pipe 4, and a second discharge pipe 5;

[0044] The axial direction of the tank body 1 extends vertically; in this embodiment, the tank body 1 is cylindrical.

[0045] The conical baffle 2 is connected to the bottom of the tank body 1 and forms a liquid storage cavity with the tank body 1; the conical baffle 2 extends from the center of the tank body 1 downwards to the inner wall surface of the tank body 1.

[0046] The sedimentation baffle 3 is disposed in the liquid storage chamber; the sedimentation baffle 3 is spiral in shape, preferably, the sedimentation baffle 3 is a conical spiral structure extending from top to bottom; the sedimentation baffle 3 extends spirally around the axis of the tank body 1 and from the center of the tank body 1 to the inner wall surface of the tank body 1; along the axial direction of the tank body 1, one end of the sedimentation baffle 3 is connected to the conical baffle 2.

[0047] The sedimentation baffle 3, the conical baffle 2, and the inner wall of the tank 1 form a spiral sediment-containing channel a;

[0048] In this embodiment, the conical baffle 2 is a conical structure that is high in the middle and low at the edges.

[0049] The first discharge pipe 4 is provided at the center of the conical baffle 2. The input end of the first discharge pipe 4 is inserted into the conical baffle 2 along the axial direction of the tank body 1. Its input end opening is located at the center of the conical baffle 2. The input end of the first discharge pipe 4 is located in the sediment receiving channel a, and its output end opening is located outside the tank body 1. The first discharge pipe 4 is used to discharge the clarified antigen solution in the storage chamber.

[0050] The outer edge of the conical baffle 2 is provided with the second discharge pipe 5. The input end of the second discharge pipe 5 is inserted into the sediment containing channel a along the axial direction of the tank body 1, and the output end is located outside the liquid storage chamber. The second discharge pipe 5 is used to discharge the sediment in the sediment containing channel a.

[0051] Under the above design, the height of the input end of the first discharge pipe 4 in the liquid storage chamber is greater than the height of the input end of the second discharge pipe 5, so that the input end of the first discharge pipe 4 can only obtain the clarified antigen liquid in the upper part of the liquid storage chamber, thereby preventing the precipitate from being discharged directly from the first discharge pipe 4; at the same time, the input end of the second discharge pipe 5 should be located at the lowest point of the liquid storage chamber, so that the precipitate can be completely discharged from the second discharge pipe 5.

[0052] In practical applications, the sedimentation baffle 3 is positioned at an interval from the inner wall of the tank 1 along its spiral direction, away from the center of the tank 1, forming a first gap b. This design avoids direct contact between the sedimentation baffle 3 and the tank 1, preventing the corners of the sediment receiving channel a from being unable to be cleaned, thus preventing sediment from remaining in the liquid storage chamber.

[0053] In this embodiment, preferably, there are two second discharge pipes 5, which are arranged at intervals around the axis of the tank body 1. Specifically, both second discharge pipes 5 are located at the first gap b and are symmetrically arranged on one side of the first discharge pipe 4. This design enables rapid discharge of sediment.

[0054] In practical applications, the antigen purification storage tank also includes an input pipe 6, which is connected to the top of the tank body 1 and communicates with the liquid storage chamber. There are multiple input pipes 6, arranged circumferentially around the axis of the tank body 1. It should be noted that in this embodiment, there are six input pipes 6. Those skilled in the art can design the actual number of input pipes 6 according to the required number of substances to be introduced.

[0055] In this embodiment, a manhole is provided at the top of the tank 1. A manhole is an opening structure for personnel to enter and exit the equipment for installation, maintenance, and safety inspection. It mainly consists of a short cylindrical section (or short pipe), a flange, and a manhole cover with a handle. Furthermore, to facilitate observation and movement of the tank 1 by personnel, a viewing window 101 and a handle 102 are provided on the outer peripheral wall of the tank 1, and casters 103 are provided at the bottom of the tank 1.

[0056] The method of using the antigen removal storage tank in this embodiment includes the following steps:

[0057] Step (1): Let the antigen impurity removal storage tank containing the vaccine antigen stock solution stand for a certain period of time until the vaccine antigen stock solution forms a clear liquid layer and a precipitate layer, wherein cell fragments or egg yolk and other impurities in the vaccine antigen stock solution fall into the precipitate receiving channel a to form the precipitate layer.

[0058] Step (2): Open only the first discharge pipe 4 to discharge the clarified antigen solution in the clarified liquid layer for use; during the process, the precipitate (mainly cell fragments or egg yolk and other impurities) will enter the precipitate receiving channel a and accumulate on the inner wall of the precipitate receiving channel a, achieving a certain obstruction effect, so as to reduce the content of precipitate in the discharged vaccine antigen stock solution and improve the purity of effective antigen; at the same time, the precipitation baffle 3 in the storage chamber can also change the fluid movement state, prevent the generation of eddies, reduce the risk of precipitate being carried away by the fluid, and further reduce the content of precipitate in the vaccine antigen stock solution taken under the dual effect.

[0059] Step (3): After the liquid level of the clarified liquid layer drops below the inlet opening of the first discharge pipe 4, the second discharge outlet 5 is opened to drain the precipitate layer.

[0060] This embodiment also includes the following steps for cleaning tank 1:

[0061] Step (4): Connect the output end of the first discharge pipe 4 to the cleaning liquid water source, and then introduce the cleaning liquid into the storage chamber through the first discharge pipe 4 and control the liquid inlet rate and liquid inlet volume so that the cleaning liquid flows back after gushing out and flushing, flows along the sediment containing channel a and is discharged from the second discharge outlet 5.

[0062] Step (5): After a preset time, close the second outlet 5, then introduce cleaning fluid until the reservoir is full of cleaning fluid, then open the second outlet 5 to completely drain the cleaning fluid. It should be noted that the preset time is set according to actual needs and is not limited in this embodiment.

[0063] This method of use can effectively reduce the contamination of impurities such as egg yolk and cell debris when collecting antigens, and at the same time, avoid the waste caused by having to discard too much antigen in order to obtain clear antigens.

[0064] Example 2

[0065] like Figure 6 , 7 As shown, an embodiment of the present invention provides an antigen removal storage tank, the structure of which is basically the same as that of embodiment 1. Preferably, the difference is that: along the axial direction of the tank body 1, the end of the sedimentation baffle 3 away from the conical baffle 2 is flush with the end of the sedimentation baffle 3 away from the conical baffle 2, and the central starting end of the sedimentation baffle 3 is flush with and connected to the inlet opening of the first discharge pipe 4.

[0066] Compared with Example 1, this embodiment optimizes the spiral sediment-accepting channel a formed between the sedimentation baffle 3, the conical baffle 2, and the inner wall of the tank 1. On the one hand, the sediment-accepting channel a has a larger volume. During use, since the top of the baffle is flush with the liquid surface that needs to be discarded due to the accumulation of sediment, the sediment can be minimized from being rolled up by the eddy current and passing over the baffle into the opening of the first discharge pipe, thus preventing the clear antigen solution from being mixed with impurities again.

[0067] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. An antigen purification storage container, characterized in that, Includes a tank body, a conical baffle, a sedimentation baffle, a first discharge pipe, and a second discharge pipe; The axial direction of the tank body extends vertically; The conical baffle is connected to the bottom of the tank and forms a liquid storage cavity with the tank; the conical baffle extends from the center of the tank downwards to the inner wall of the tank. The sedimentation baffle is disposed in the liquid storage chamber; the sedimentation baffle is spiral-shaped and extends spirally from the center of the tank to the inner wall surface of the tank around the axis of the tank body; along the axis of the tank body, one end of the sedimentation baffle is connected to the conical baffle; the sedimentation baffle, the conical baffle and the inner wall surface of the tank body form a spiral sediment receiving channel; in, The first discharge pipe is located at the center of the conical baffle. The first discharge pipe is inserted into the conical baffle along the axial direction of the tank body. Its input end opening is located at the center of the conical baffle, and its output end opening is located outside the tank body. The first discharge pipe is used to discharge the clarified antigen solution in the storage chamber. The outer edge of the conical baffle is provided with a second discharge pipe. The input end of the second discharge pipe is inserted into the sediment containing channel along the axial direction of the tank body, and its output end opening is located outside the tank body. The second discharge pipe is used to discharge the sediment in the sediment containing channel.

2. The antigen purification storage tank according to claim 1, characterized in that, The sedimentation baffle is a conical spiral structure extending from top to bottom.

3. The antigen purification storage container according to claim 1, characterized in that, Along the axial direction of the tank, the end of the sedimentation baffle away from the conical baffle is flush with the center starting end of the sedimentation baffle and is flush with and connected to the inlet opening of the first discharge pipe.

4. The antigen purification storage container according to any one of claims 1 to 3, characterized in that, The sedimentation baffle is arranged at an interval from the inner wall of the tank along its spiral direction, forming a first gap.

5. The antigen purification storage tank according to claim 4, characterized in that, There are multiple second discharge pipes, which are arranged at intervals around the axis of the tank.

6. The antigen purification storage tank according to claim 5, characterized in that, At least one second discharge pipe is provided at the first gap.

7. The antigen purification storage tank according to claim 1, characterized in that, The antigen removal storage tank also includes an input pipe, which is connected to the top of the tank and communicates with the liquid storage chamber. There are multiple input pipes, which are arranged circumferentially around the axis of the tank.

8. The antigen purification storage tank according to claim 1, characterized in that, The top of the tank is provided with a manhole, the outer peripheral wall of the tank is provided with a viewing window and a handle, and the bottom of the tank is provided with casters.

9. A method of using an antigen purification storage container, characterized in that, Based on the antigen removal storage tank as described in any one of claims 1 to 8, the process includes the following steps: Step (1): Let the antigen impurity storage tank containing the vaccine antigen stock solution stand until the vaccine antigen stock solution forms a clear liquid layer and a precipitate layer, wherein cell fragments or egg yolk and other impurities in the vaccine antigen stock solution fall into the precipitate containing channel to form the precipitate layer. Step (2): Open only the first discharge pipe to take the clarified liquid layer; Step (3): After the liquid level of the clarified liquid layer drops below the opening of the inlet of the first discharge pipe, open the second discharge pipe to drain the precipitate layer.

10. The method of using the antigen purification storage container according to claim 9, characterized in that, It also includes the following steps: Step (4): Connect the output end of the first discharge pipe to the cleaning liquid water source, and then introduce the cleaning liquid into the storage chamber through the first discharge pipe and control the liquid inlet rate and liquid inlet volume so that after the cleaning liquid gushes out and falls back to flush, it flows along the sediment containing channel and is discharged from the second discharge pipe. Step (5): After a preset time, close the second discharge pipe, then introduce cleaning fluid until the reservoir is full of cleaning fluid, then open the second discharge pipe to completely drain the cleaning fluid.

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