Micro-current dry-state soothing anti-allergy mask and preparation method thereof

Abstract

The invention relates to the field of daily chemicals, and discloses a micro-current dry-state soothing anti-allergy mask and a preparation method thereof.The micro-current dry-state soothing anti-allergy mask comprises a soothing anti-allergy functional layer and a friction power generation layer which are attached to each other; the soothing anti-allergy functional layer comprises a skin-core type nanofiber membrane and a conductive pattern; a skin layer of a single fiber in the skin-core nanofiber membrane is water-soluble silk fibroin loaded with sorbitol and essence components, and a core layer is water-soluble silk fibroin loaded with a soothing and anti-allergy plant extract composition; according to the principle of generating electricity through friction, micro-current massage in the mask applying process can be achieved under the condition that external equipment is not needed; the plant extract composition is loaded on the core layer of the nanofiber membrane by virtue of a coaxial electrostatic spinning technology, so that the influence of long-term contact between active ingredients in the plant extract composition and other ingredients in the essence can be avoided, the activity stability of the essence is improved, and the soothing and anti-allergy effects are improved.

Description

Technical Field

[0001] This invention relates to the field of functional materials, and in particular to a microcurrent dry-state soothing and anti-allergic facial mask and its preparation method. Background Technology

[0002] Currently, most commercially available face mask products are simply mask sheets loaded with essence, resulting in a relatively limited product range. With societal development, consumers' functional needs for face masks are becoming increasingly diverse. Existing research indicates that massaging the face while applying a mask can not only promote facial blood circulation and relieve facial tightness, thus providing a stress-relieving and soothing effect, but also enhance the skin's transdermal absorption of the essence, thereby better releasing the efficacy of the active ingredients in the essence.

[0003] Against this backdrop, various microcurrent massagers and microcurrent massage masks have gradually appeared on the market in recent years. For example, Chinese patent CN202011233612.7 discloses a microcurrent massage mask, which uses silver paste to print conductive patterns on a mask sheet, and then loads the mask sheet with essence to form the mask. When the mask is applied, a beauty device provides microcurrent (through the conductive patterns). It describes the effect of the microcurrent, which can promote facial blood circulation and improve the absorption rate of the essence, thereby achieving a long-term moisturizing effect on the facial skin. However, the disadvantage of the above-mentioned mask is that it requires an external beauty device to generate microcurrent, making the operation relatively complicated and inconvenient to carry.

[0004] On the other hand, facial skin is highly sensitive, and frequent exposure to sunlight or various allergens can easily lead to facial allergies. Therefore, consumer demand for soothing and anti-allergic facial masks is increasing. The applicant's preliminary research has found that a combination of three specific plant extracts—chamomile extract, poria cocos extract, and purslane extract—exhibits a synergistic effect in soothing and anti-allergic properties compared to any single extract. Therefore, incorporating this plant extract combination as an active ingredient in a facial mask could meet current market demand and has significant market potential.

[0005] However, when we tried to formulate the above-mentioned plant extract composition into a serum and load it onto the mask base, we found that the effect was not as expected. After a series of analyses, we found the main reason: due to the complexity of the serum formula and the relatively poor stability of the active ingredients in the above-mentioned plant extract composition, after a certain period of storage, the active ingredients in the plant extract composition are easily affected by other components in the serum and the pH of the system, thus affecting the soothing and anti-allergic effects. Therefore, developing a mask product that can maintain the activity of the above-mentioned plant extract composition for a long time has become a challenge. Summary of the Invention

[0006] To overcome the aforementioned technical problems, this invention provides a microcurrent dry-state soothing and anti-allergic facial mask and its preparation method. First, based on the principle of triboelectricity, this invention enables microcurrent massage during mask application without the need for external equipment. Second, by utilizing coaxial electrospinning technology to load a plant extract composition onto the core layer of a nanofiber membrane, this invention avoids long-term contact between the active ingredients in the plant extract composition and other components in the essence, improving their activity stability and thus enhancing the soothing and anti-allergic effect.

[0007] The specific technical solution of this invention is as follows: First, this invention provides a microcurrent dry-state soothing and anti-allergic facial mask, comprising a release film, a soothing and anti-allergic functional layer, and a triboelectric layer sequentially bonded together; the soothing and anti-allergic functional layer faces the skin during use, and the triboelectric layer is located in the area of ​​the cheek corresponding to the soothing and anti-allergic functional layer. Wherein: The soothing and anti-allergic functional layer comprises a core-sheath type nanofiber membrane serving as the mask base and conductive patterns distributed on the core-sheath type nanofiber membrane. In the core-sheath type nanofiber membrane, the sheath layer of each individual fiber is a water-soluble silk fibroin loaded with sorbitol and essential ingredients, while the core layer is a water-soluble silk fibroin loaded with a composition of soothing and anti-allergic plant extracts. The composition of soothing and anti-allergic plant extracts includes chamomile extract, poria cocos extract, and purslane extract. More preferably, the poria cocos extract and purslane extract are aqueous extracts or alcohol-water extracts.

[0008] This invention uses chamomile extract, poria cocos extract, and purslane extract as the main active ingredients in a facial mask. These were chosen because preliminary experiments revealed good compatibility among these three extracts. Combining them in appropriate proportions produces a synergistic effect in soothing and reducing skin sensitivity. Furthermore, this invention found that replacing one or two of these extracts with other substances with similar effects does not achieve the same level of synergistic effect. Further research and analysis revealed that their synergistic mechanism may be as follows: First, compared to single-pathway effects, the three active substances in this invention exert their soothing and anti-allergic effects through different pathways. Specifically, chamomile contains abundant polyphenols, which can reduce ROS levels and downregulate T cell activation, migration, and differentiation, thus exerting a soothing and anti-allergic effect; Poria cocos contains abundant polysaccharides, which can inhibit cyclooxygenase (COX) activity and suppress the release of inflammatory factors such as prostaglandins and leukotrienes; moreover, polysaccharides have good moisturizing and barrier repair effects on the skin; and the flavonoids in purslane can inhibit trypsin secretion and mast cell degranulation, thereby achieving a soothing effect.

[0009] Secondly, chamomile, poria cocos, and purslane, while exerting their individual effects, also have a synergistic effect: purslane extract, while inhibiting mast cell degranulation to reduce skin sensitivity, can also significantly enhance the downregulation of pro-inflammatory factors by poria cocos polysaccharides, thus reducing inflammatory responses; simultaneously, poria cocos extract, while reducing the release of inflammatory factors, can also enhance the function of chamomile extract in scavenging ROS and reducing the body's oxidative stress response. In summary, this invention, by combining purslane, chamomile, and poria cocos, achieves a long-lasting and significant anti-inflammatory and soothing effect.

[0010] Building upon this foundation, to ensure the bioactivity of the aforementioned soothing and anti-allergic plant extract composition, this invention utilizes coaxial electrospinning technology to encapsulate it within the core layer of water-soluble silk fibroin nanofibers. Other essential components of the serum are loaded into the dermis, achieving physical isolation. The entire mask is in a dry state, requiring no liquid serum. When using the mask, simply add water and apply it to the face. Upon contact with water, the water-soluble silk fibroin swells or dissolves, releasing the substances it carries, which are then absorbed by the skin.

[0011] Furthermore, it should be noted that because the molecular conformation of water-soluble silk fibroin is mainly random coil, it will swell or dissolve rapidly upon contact with water. This is not a problem for ordinary face masks, but because the base fabric of the mask in this invention is printed with conductive patterns and has a triboelectric layer, excessive dissolution of the silk fibroin will cause the conductive patterns and triboelectric layer to lose their adhesion to the substrate, affecting normal use and making it difficult to peel off the entire mask after use. To address this, this invention cleverly disrupts the interaction between silk fibroin and water molecules by doping a portion of sorbitol into the outer layer of the nanofibers, and induces the silk fibroin molecules to transform from random coil to β-sheet structure, forming a more compact aggregate state, thereby reducing its dissolution rate in water and ensuring that the mask maintains a certain strength during application.

[0012] The triboelectric layer comprises a hydrophobic electron donor layer, an elastic spacer layer, and an electron acceptor layer bonded together in sequence; the hydrophobic electron donor layer is bonded to a core-shell nanofiber membrane; and the hydrophobic electron donor layer and the electron acceptor layer are electrically connected to a conductive pattern, respectively.

[0013] The triboelectric power generation layer of this invention is based on existing triboelectric nanogenerators (TENGs) and adapted to the characteristics of facial masks. Its working principle is as follows: After applying the mask, gently and repeatedly press the outermost electron-receiving layer of the triboelectric power generation layer. During this pressing process, the elastic spacer layer in the middle is repeatedly compressed and recovered. The electron-receiving layer and the hydrophobic electron-donating layer repeatedly contact and separate. During this process, electrons transfer from the electron-receiving layer to the hydrophobic electron-donating layer, making them positively and negatively charged respectively, forming a potential difference. The microcurrent is conducted through the conductive pattern, thereby providing a microcurrent massage effect on the skin. This not only promotes facial blood circulation and relieves facial tightness, providing a stress-relieving and soothing effect, but also enhances the transdermal absorption of active substances by the facial skin. Compared to microcurrent facial masks on the market that rely on external devices, this invention's mask generates its own electricity through the principle of triboelectricity, making it more convenient to carry.

[0014] Preferably, the hydrophobic electron-donating layer is a hydrophobic polytetrafluoroethylene film with a conductive layer on the skin-facing side; the elastic spacer layer is an elastic porous silicone layer or a polyurethane porous sponge layer with through holes; and the electron-accepting layer is a nylon layer with a conductive metal plated on its surface.

[0015] Preferably, the thickness of the soothing and anti-allergy functional layer is 40-60 micrometers; the thickness of the hydrophobic electron-donating layer is 20-30 micrometers; the thickness of the elastic spacer layer is 40-60 micrometers; and the thickness of the electron-accepting layer is 20-30 micrometers.

[0016] Preferably, the conductive pattern is applied to the skin-back side of the core-shell nanofiber membrane using PEDOT:PSS as the raw material by printing; the conductive layer in the hydrophobic electron-donating layer is applied to the skin-facing side of the hydrophobic polytetrafluoroethylene membrane using PEDOT:PSS as the raw material by printing.

[0017] Preferably, the conductive pattern is selected from one or more shapes such as grid, honeycomb, line, spiral, interdigitated and comb.

[0018] Secondly, this invention provides a method for preparing a dry soothing and anti-allergic facial mask, which includes the following steps: S1: Preparation of the soothing and anti-allergic functional layer: Water-soluble silk fibroin, sorbitol and essence ingredients are added to water to obtain the skin layer spinning solution; a combination of water-soluble silk fibroin and soothing and anti-allergic plant extracts is added to water to obtain the core layer spinning solution; a skin-core type nanofiber membrane is prepared by coaxial electrospinning technology, the solvent is dried and evaporated, the shape is cut, and then conductive patterns are printed on the skin-core type nanofiber membrane to obtain the soothing and anti-allergic functional layer and a release film is attached.

[0019] S2: The hydrophobic electron-donating layer of the assembled triboelectric layer is bonded to the side of the core-shell nanofiber membrane printed with conductive patterns, ensuring that the conductive patterns are in contact with the hydrophobic electron-donating layer. Finally, the electron-accepting layer is connected to the conductive patterns by sewing conductive fibers to obtain a microcurrent dry-state soothing and anti-allergic mask.

[0020] Preferably, in S1, the mass ratio of chamomile extract, poria cocos extract and purslane extract in the soothing and anti-allergic plant extract composition is 100:(10-30):(30-50).

[0021] Preferably, in S1, the content of water-soluble silk fibroin in the dermal spinning solution is 10-15 wt%, the content of sorbitol is 1-3 wt%, and the total content of the essence ingredients is 0.1-3 wt%; in the core spinning solution, the content of water-soluble silk fibroin is 6-8 wt%, and the content of the soothing and anti-allergic plant extract composition is 0.5-1.5 wt%.

[0022] To successfully prepare core-sheath structured nanofiber membranes, this invention strictly controls the concentration of water-soluble silk fibroin in the sheath spinning solution to be higher than that in the core layer, ensuring that the sheath spinning solution has a higher viscosity. This prevents the core layer spinning solution from penetrating into the sheath layer during electrospinning. At the same time, by utilizing the difference in water evaporation rates, the sheath structure is preferentially formed, thereby achieving the encapsulation of the core layer.

[0023] On the other hand, as mentioned earlier, sorbitol can slow down the dissolution rate of water-soluble silk fibroin in water, so its content is quite critical. If its content is too low, it cannot effectively slow down the dissolution of water-soluble silk fibroin; if its content is too high, it will prevent the active substances from being released quickly. After repeated experiments, it was found that controlling the sorbitol content within the above range is optimal, and sorbitol is only added to the skin layer, not the core layer.

[0024] Preferably, the essence ingredients include one or more of moisturizers, thickeners, and pH adjusters.

[0025] More preferably, the moisturizer is selected from one or more of hyaluronic acid, sodium hyaluronate, and panthenol; the thickener is selected from one or more of xanthan gum, carbomer, and small core bacteria gum; and the pH adjuster is arginine, which enables the mask to become weakly acidic after being mixed with water, matching the pH of the skin.

[0026] Preferably, in S1, the conditions for coaxial electrospinning are as follows: the flow rate of the sheath spinning solution is 0.005-0.01 mm / s, the flow rate ratio of the sheath spinning solution to the core spinning solution is 5-10:1; the temperature is 25-40℃, the relative humidity is 40-50%; the sheath needle is 15-20g, the core needle is 20-25g, the receiving distance is 15-18 cm, the receiver rotation speed is 500-1500 rpm, and the voltage is 18-22 kV.

[0027] To better form the core-sheath structure, the flow rate ratio of the sheath spinning solution and the core spinning solution needs to be optimized. In addition, since the solvent for electrospinning in this invention is water, which is less volatile than alcohol solvents, it is necessary to appropriately reduce the humidity (excessive ambient humidity will delay water evaporation, making it difficult for the core-sheath to solidify quickly).

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The present invention uses chamomile extract, poria cocos extract and purslane extract as the main active ingredients of the mask. The compatibility of the above three extracts is excellent. When they are combined in an appropriate ratio, they can produce a synergistic effect of "1+1+1>3" in terms of skin soothing and anti-allergy.

[0029] (2) This invention is based on the principle of triboelectric nanogenerator (TENG) and is designed to create a triboelectric layer tailored to the characteristics of facial masks. After the mask is applied, gentle and repeated pressing with the hands generates a microcurrent, which massages the skin and promotes facial blood circulation, relieves facial tightness, and provides stress relief. It also enhances the skin's transdermal absorption of active ingredients. Compared to microcurrent facial mask products on the market that rely on external devices, this invention generates its own electricity using the principle of triboelectricity, making it more convenient to carry.

[0030] (3) This invention utilizes coaxial electrospinning technology to encapsulate the soothing and anti-allergic plant extract composition and other essential components of the essence within the core and outer layers of water-soluble silk fibroin nanofibers, achieving physical isolation and improving the activity stability of the soothing and anti-allergic plant extract composition. The entire mask is in a dry state, requiring no liquid essence. When using the mask, simply add water and apply it. The water-soluble silk fibroin releases active substances upon contact with water, which are then absorbed by the skin.

[0031] (4) The present invention slows down the dissolution rate of water-soluble silk fibroin in water by doping an appropriate amount of sorbitol into the skin layer of water-soluble silk fibroin nanofibers, so as to ensure that the mask maintains a certain strength during the application process. Attached Figure Description

[0032] Figure 1 This is an image showing the appearance of the microcurrent dry-state soothing and anti-allergic facial mask of the present invention. Figure 2 This is a schematic diagram of the layered structure of the triboelectric layer in the microcurrent dry-state soothing and anti-allergic face mask of the present invention; Figure 3 ROS fluorescence images of the blank group, model group, and experimental group 7; Figure 4 The effects of experimental groups 1-10 on ROS levels in zebrafish; Figure 5The effect of experimental groups 1-10 on the relative expression level of COX-2 gene in zebrafish; Figure 6 The effect of experimental groups 1-10 on the relative expression level of TNF-α gene in zebrafish; Figure 7 The effect of experimental groups 1-10 on the relative expression level of IL-6 gene in zebrafish; Figure 8 Images showing reddened facial skin on the subject (left: before use, right: after use). Detailed Implementation

[0033] The present invention will be further described below with reference to embodiments.

[0034] First, a microcurrent dry-state soothing and anti-allergic facial mask includes a release film, a soothing and anti-allergic functional layer, and a triboelectric layer sequentially bonded together. The soothing and anti-allergic functional layer faces the skin during use, and the triboelectric layer is located in the area of ​​the cheek corresponding to the soothing and anti-allergic functional layer. Specifically, the soothing and anti-allergic functional layer includes a core-skin type nanofiber membrane serving as the mask base and conductive patterns distributed on the core-skin type nanofiber membrane. The sheath of each fiber in the core-skin type nanofiber membrane is a water-soluble silk fibroin loaded with sorbitol and essential ingredients, while the core layer is a water-soluble silk fibroin loaded with a composition of soothing and anti-allergic plant extracts. The composition of soothing and anti-allergic plant extracts includes chamomile extract, poria cocos extract, and purslane extract. In some embodiments, the poria cocos extract and purslane extract are aqueous extracts or alcohol-water extracts.

[0035] The triboelectric layer comprises a hydrophobic electron donor layer, an elastic spacer layer, and an electron acceptor layer bonded together in sequence; the hydrophobic electron donor layer is bonded to a core-shell nanofiber membrane; the hydrophobic electron donor layer and the electron acceptor layer are electrically connected to a conductive pattern (preferably distributed at least in the cheek and forehead areas).

[0036] In some implementation cases, the hydrophobic electron-donating layer is a hydrophobic polytetrafluoroethylene film with a conductive layer on the skin-facing side; the elastic spacer layer is an elastic porous silicone layer or a polyurethane porous sponge layer with through holes; and the electron-accepting layer is a nylon layer with a conductive metal plated on its surface.

[0037] In some implementation cases, the thickness of the soothing and anti-allergic functional layer is 40-60 micrometers; the thickness of the hydrophobic electron-donating layer is 20-30 micrometers; the thickness of the elastic spacer layer is 40-60 micrometers; and the thickness of the electron-accepting layer is 20-30 micrometers.

[0038] In some implementation cases, the conductive pattern is attached to the skin-back side of the core-shell nanofiber membrane using PEDOT:PSS as the raw material by printing; the conductive layer in the hydrophobic electron-donating layer is attached to the skin-facing side of the hydrophobic polytetrafluoroethylene membrane using PEDOT:PSS as the raw material by printing.

[0039] In some implementations, the conductive pattern is selected from one or more shapes such as grid, honeycomb, line, spiral, interdigitated and comb.

[0040] Secondly, this invention provides a method for preparing a dry soothing and anti-allergic facial mask, which includes the following steps: S1: Preparation of the soothing and anti-allergic functional layer: Water-soluble silk fibroin, sorbitol and essence ingredients are added to water to obtain the skin layer spinning solution; a combination of water-soluble silk fibroin and soothing and anti-allergic plant extracts is added to water to obtain the core layer spinning solution; a skin-core type nanofiber membrane is prepared by coaxial electrospinning technology, the solvent is dried and evaporated, the shape is cut, and then conductive patterns are printed on the skin-core type nanofiber membrane to obtain the soothing and anti-allergic functional layer and a release film is attached.

[0041] In some implementation cases, the mass ratio of chamomile extract, poria cocos extract and purslane extract in the soothing and anti-allergic plant extract composition is 100:(10-30):(30-50).

[0042] In some implementation cases, the content of water-soluble silk fibroin in the dermal spinning solution is 10-15 wt%, the content of sorbitol is 1-3 wt%, and the total content of the essence ingredients is 0.1-3 wt%; in the core spinning solution, the content of water-soluble silk fibroin is 6-8 wt%, and the content of the soothing and anti-allergic plant extract composition is 0.5-1.5 wt%.

[0043] In some implementation cases, the essence ingredients include one or more of moisturizers, thickeners, and pH adjusters; more preferably, the moisturizer is selected from one or more of hyaluronic acid, sodium hyaluronate, and panthenol; the thickener is selected from one or more of xanthan gum, carbomer, and small core bacteria gum; and the pH adjuster is arginine, which enables the mask to become weakly acidic after being mixed with water, matching the pH of the skin.

[0044] In some implementation cases, the conditions for coaxial electrospinning are as follows: the flow rate of the sheath spinning solution is 0.005-0.01 mm / s, the flow rate ratio of the sheath spinning solution to the core spinning solution is 5-10:1; the temperature is 25-40℃, the relative humidity is 40-50%; the sheath needle is 15-20g, the core needle is 20-25g, the receiving distance is 15-18 cm, the receiver rotation speed is 500-1500 rpm, and the voltage is 18-22 kV.

[0045] S2: The hydrophobic electron-donating layer of the assembled triboelectric layer is bonded to the side of the core-shell nanofiber membrane printed with conductive patterns, ensuring that the conductive patterns are in contact with the hydrophobic electron-donating layer. Finally, the electron-accepting layer is connected to the conductive patterns by sewing conductive fibers (ultimately, a circuit is formed between the electron-accepting layer, the hydrophobic electron-donating layer, and the conductive patterns), thus producing a microcurrent dry-state soothing and anti-allergic mask.

[0046] (a) The effect of different extract combinations on soothing and anti-allergic effects Table 1 Weigh each raw material according to the proportions in Table 1, mix them, and obtain the corresponding soothing and anti-sickness composition.

[0047] Among them, chamomile water extract was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-045; Poria cocos water extract was purchased from Shaanxi Bolin Biotechnology Co., Ltd.; Portulaca oleracea water extract was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-241; green tea water extract (as a similar efficacy substitute for chamomile water extract) was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-120; oat water extract (as a similar efficacy substitute for Poria cocos water extract) was purchased from Shaanxi Pinhong Biotechnology Co., Ltd.; and Centella asiatica water extract (as a similar efficacy substitute for Portulaca oleracea water extract) was purchased from Hunan Langlin Bio-resources Co., Ltd., NAT-116.

[0048] Efficacy testing (1) Anti-allergic activity test (determination of tryptase inhibition rate in zebrafish) Principle: Zebrafish mast cells, with structures and functions similar to those of mammals, participate in the body's immune and allergic responses. N-benzoyl-DL-arginine p-nitrobenzamide hydrochloride (BAPNA) is a tryptase-specific substrate, allowing for the detection of tryptase expression levels in zebrafish. Measuring the tryptase expression level and inhibition rate induced by a substance in zebrafish over 24 hours allows for quantitative analysis of the substance's sensitizing and anti-allergic efficacy.

[0049] S1: Each group of soothing and anti-allergic composition solutions (1.0 mg / mL) was prepared using distilled water as the sample group; a Compoud 48 / 80 solution (10 μg / mL) was prepared using distilled water as the model control group; and distilled water was used as the solvent control group.

[0050] S2: Select zebrafish with normal development and 3 dpf (wild AB type zebrafish, Nanjing Yishu Lihua Biotechnology Co., Ltd.) and place them in 96-well plates, 5 zebrafish per well. Add 100 μL of distilled water to the model control group and 100 μL of each group's soothing and anti-allergic composition solution to the sample group. Set up 4 replicates for each group and incubate at 28℃ for 1 h.

[0051] S3: Add 100 μL of compoud 48 / 80 solution to each well and incubate at 28 °C for 1 h.

[0052] S4: Take 100 μL of supernatant from each well and place it in another 96-well plate. Add 100 μL of BAPNA (1 mg / mL) and incubate at 38°C for 1 h.

[0053] S5: Use an ELISA reader to detect the absorbance (OD405 value) and calculate the Tryptase expression level using the following formula.

[0054] Tryptase expression level / % = (OD 样品组 - OD 溶剂组 ) / OD 溶剂组 ×100% Tryptase inhibition rate / % = (Tryptase 模型组 - Tryptase 样品组 Tryptase 模型组 ×100% The data from the above tests are shown in Table 2: Table 2 The data comparison in Table 2 shows that the soothing and anti-allergic compositions in each experimental group can reduce the amount of tryptase secretion caused by compoud 48 / 80 stimulation to a certain extent. Among them: Experimental groups 1-3 used a single extract, while experimental groups 4-6 used two extracts each. Compared with experimental group 7, which used a combination of three extracts (chamomile + poria cocos water + purslane), the results showed that the combination of "chamomile + poria cocos water + purslane" was more advantageous, indicating that these three specific extracts can produce a synergistic effect.

[0055] In experimental groups 8-10, one of the extracts in experimental group 7 was replaced with an extract that is currently recognized as having similar efficacy. The results showed that the inhibition rate of Tryptase in experimental groups 8-10 was significantly lower than that in experimental group 7, indicating that the types of the three plant extracts in the composition of the present invention cannot be simply replaced.

[0056] (2) Antioxidant activity test (ROS production in zebrafish) Zebrafish embryos with normal development 24 h after fertilization were selected and randomly divided into three groups: a blank control group (supplemented with culture water only), a model group (2.0 mmol / L H2O2), and 10 experimental groups (2.0 mmol / L H2O2 + 1.0 mg / mL, experimental groups 1-10). Each well contained 20 embryos, and each group had 3 parallel wells. All groups were cultured at 28.5℃ in a light-dark incubator with a 14 h / 10 h light-dark cycle for 96 h, with the solution changed every 24 h.

[0057] After culture, the embryos were first washed with zebrafish culture water, and then stained with DCFH-DA (20 μg / mL) for 1.2 h (in the dark). After staining, the embryos were washed again with zebrafish culture water, and then anesthetized with 0.02% 3-ethoxyaniline methanesulfonate. The fluorescence intensity in the zebrafish was then observed under a stereofluorescence microscope, and the fluorescence intensity of the samples was measured using ImageJ software to analyze the amount of ROS produced in the samples.

[0058] pass Figure 3 and Figure 4 Data comparison shows that the soothing and anti-allergic compositions in each experimental group can reduce ROS produced by H2O2 stimulation in zebrafish to a certain extent, and decrease fluorescence intensity. Specifically: Experimental groups 1-3 used a single extract, while experimental groups 4-6 used two extracts each. Compared with experimental group 7, which used a combination of three extracts (chamomile + poria cocos water + purslane), the results showed that the combination of "chamomile + poria cocos water + purslane" was more advantageous, indicating that these three specific extracts can produce a synergistic effect.

[0059] In experimental groups 8-10, one of the extracts in experimental group 7 was replaced with an extract that is currently recognized as having similar efficacy. The results showed that the ability of experimental groups 8-10 to clear ROS generated in zebrafish was significantly lower than that of experimental group 7, indicating that the types of the three plant extracts in the composition of the present invention cannot be simply replaced.

[0060] (3) Anti-inflammatory activity test (testing of COX-2 activity and inflammatory factors in zebrafish) Zebrafish embryos with normal development were selected 7-8 hours after fertilization. The experiment was divided into a blank group (only culture water was added), a model group (15 μg / mL LPS), and a drug treatment group (15 μg / mL LPS + 1.0 mg / mL experimental group 1-10), with 60 embryos in each group. After incubation for 72 hours, the zebrafish embryos were collected, total RNA was extracted, cDNA was reverse transcribed, genes were amplified, and the relative expression levels of COX-2, TNF-α, and IL-6 genes were measured.

[0061] COX-2 is an inducible enzyme that is highly expressed under inflammatory stimuli and dominates inflammatory responses such as pain, fever, and swelling; interleukin-6 (IL-6) is a multifunctional cytokine produced by various cells (including lymphocytes, monocytes, macrophages, etc.) and is widely involved in the regulation of immune and inflammatory responses; an increase in the gene expression level of tumor necrosis factor-α (TNF-α) in the blood also indicates the presence of an inflammatory response.

[0062] By Figure 5 , Figure 6 and Figure 7 Data comparison shows that the soothing and anti-allergic compositions of each test group can reduce the release of inflammatory factors such as COX-2, TNF-α, and IL-6 generated by LPS stimulation in zebrafish to a certain extent. Among them: Test groups 1-3 used single extracts respectively, and test groups 4-6 used two extracts respectively. Compared with test group 7 that used a combination of three extracts (chamomile + tuckahoe water + purslane), the results showed that the combination of "chamomile + tuckahoe water + purslane" had more advantages, indicating that synergistic effects can be produced among these three specific extracts.

[0063] In test groups 8-10, one of the extracts in test group 7 was replaced with an extract that is currently公认 to have similar efficacy. The results showed that the ability of test groups 8-10 to down-regulate inflammatory factors in zebrafish was significantly lower than that of test group 7, indicating that the types of the three plant extracts in the composition of the present invention cannot be simply replaced.

[0064] (3) Skin irritation test Eight-week-old male SD rats (provided by Shanghai Slake Experimental Animal Co., Ltd., production license number: SCXK (Shanghai) 2017-0005) were selected, and the hair on both sides of the spine on their backs was shaved off (ensuring not to damage the epidermis, with an area of 3 cm × 3 cm). After 24 hours, 0.5 mL of the soothing and anti-allergic composition solution of test group 7 was applied to the epidermis on one side of the depilated area, first covered with gauze and cellophane, and then fixed with non-irritating adhesive tape and bandage. The epidermis on the other side was used as a control. After 4 hours, the covering was removed, and the epidermis was washed with warm water. The epidermis condition of the application site was observed and scored at 1 h, 24 h, 48 h, and 72 h after washing, and a comprehensive evaluation was made based on the average value of the scores of the test animals. According to the highest average score at the three time points of 24 h, 48 h, and 72 h, the skin irritation intensity was determined.

[0065] Among them, the criteria for classifying skin irritation intensity are: average score < 0.5, non-irritating; average score 0.5 to < 2.0, slightly irritating; average score 2.0 to < 6.0, moderately irritating; average score 6.0 to 8.0, strongly irritating.

[0066] The results of the skin irritation test are shown in Table 3: Table 3 As shown in Table 3, the soothing and anti-allergic composition solution in experimental group 7 was non-irritating to the skin on the back of rats.

[0067] (II) Application of soothing and anti-allergic plant extract compositions in facial masks Example 1: Preparation of core-shell nanofiber membranes with different sorbitol doping amounts (0-4 wt%) (1) Water-soluble silk fibroin and sorbitol were added to water to prepare a skin spinning solution. The content of water-soluble silk fibroin was 12wt% and the content of sorbitol was 0-4wt% (6 gradient groups were set: 0wt%, 0.5wt%, 1wt%, 2wt%, 3wt% and 4wt%).

[0068] (2) Water-soluble silk fibroin and blue ink (as an indicator for observing the dissolution effect) were added to water to prepare the core spinning solution. The content of water-soluble silk fibroin was 7wt% and the content of water-soluble pigment was 3wt%.

[0069] (3) Coaxial electrospinning was carried out using the sheath spinning solution and the core spinning solution as raw materials. The conditions were as follows: the flow rate of the sheath spinning solution was 0.008 mm / s, the flow rate of the core spinning solution was 0.001 mm / s; the temperature was 35℃, the relative humidity was 45%; the sheath needle was 18g, the core needle was 22g, the receiving distance was 16 cm, the receiving speed was 1000 rpm, and the voltage was 20 kV. After electrospinning, the solvent was dried and evaporated to obtain a sheath-core nanofiber membrane (thickness of 50 micrometers).

[0070] (4) Stability tests were conducted on the core-sheath nanofiber membranes with different sorbitol doping amounts (0 wt%, 0.5 wt%, 1 wt%, 2 wt%, 3 wt%, and 4 wt%) after adding water: Each group of samples (5 samples per group) was cut into squares with a side length of 13 cm (simulating a mask area of ​​150-200 cm²). 2 The sample was laid flat on a glass plate, and 30 mL of water was evenly added to the surface of each sample (simulating the volume of 20-40 mL of essence in each commercially available face mask). The sample was left to stand at room temperature for 15 minutes (simulating the application time of a face mask). The release of blue ink was observed visually (scored from 1 to 5 points based on the area of ​​seepage, with higher scores indicating more seepage). The sample was then peeled off by holding a corner with tweezers, and the experimental results were recorded. The results are shown in Table 4. Table 4 Comparing the experimental records in the table above, it can be seen that in experimental groups 1 and 2, due to the lack of sorbitol or a low doping amount, the water-soluble silk fibroin dissolved and swelled rapidly in water. Although the blue ink exudation effect was the best, it also prevented the samples from being successfully peeled off the glass plate. In experimental group 6, although all samples were successfully peeled off, the dissolution and swelling rate was too slow, and the blue ink could not dissolve quickly, which means it is not conducive to the release of active substances in the nanofiber membrane. Therefore, the preferred doping amount of sorbitol is 1-3 wt% (experimental groups 3-5), with the optimal amount being 2 wt% (experimental group 4).

[0071] Example 2 A microcurrent dry-state soothing and anti-allergic facial mask includes a release film, a soothing and anti-allergic functional layer, and a triboelectric layer sequentially bonded together; the soothing and anti-allergic functional layer faces the skin during use, and the triboelectric layer is located in the area of ​​the cheek corresponding to the soothing and anti-allergic functional layer. Figure 1 ).in: The soothing and anti-allergic functional layer includes a core-sheath nanofiber membrane (50 micrometers thick) serving as the mask base and conductive patterns (such as...) uniformly distributed on the core-sheath nanofiber membrane. Figure 1 As shown, the conductive pattern is located on the side facing away from the skin and is in the form of a grid.

[0072] In the core-skin type nanofiber membrane, the outer layer of each fiber is a water-soluble silk fibroin loaded with sorbitol and essential ingredients, while the core layer is a water-soluble silk fibroin loaded with a combination of soothing and anti-allergic plant extracts (chamomile water extract, poria cocos water extract, and purslane water extract).

[0073] like Figure 2 As shown, the triboelectric layer comprises a hydrophobic electron donor layer (25 micrometers thick, hydrophobic polytetrafluoroethylene film, with a PEDOT:PSS conductive layer printed on the skin-facing side), an elastic spacer layer (50 micrometers thick, elastic porous silicone layer), and an electron acceptor layer (25 micrometers thick, silver-plated nylon layer) bonded together in sequence; the hydrophobic electron donor layer is bonded to the skin-core type nanofiber membrane; the hydrophobic electron donor layer and the electron acceptor layer are electrically connected to the conductive pattern, respectively.

[0074] Preparation process: S1: Preparation of the soothing and anti-allergic functional layer: Water-soluble silk fibroin, sorbitol, and essential ingredients (hyaluronic acid, panthenol, xanthan gum, arginine) were added to water to prepare the dermal spinning solution. The content of water-soluble silk fibroin was 12wt%, sorbitol was 2wt%, hyaluronic acid was 0.25wt%, panthenol was 0.25wt%, xanthan gum was 0.2wt%, and arginine was 0.1wt%. A combination of water-soluble silk fibroin and soothing and anti-allergic plant extracts (chamomile water extract, poria cocos water extract, and purslane water extract in a mass ratio of 100:20:40) was added to water to prepare the core spinning solution. The content of water-soluble silk fibroin was 7wt%, and the content of the soothing and anti-allergic plant extracts was 1wt%. Coaxial electrospinning was performed using sheath-core and core-core spinning solutions as raw materials under the following conditions: sheath-core spinning solution flow rate 0.008 mm / s, core-core spinning solution flow rate 0.001 mm / s; temperature 35℃, relative humidity 45%; sheath needle 18g, core needle 22g, receiver distance 16 cm, receiver rotation speed 1000 rpm, voltage 20 kV. After electrospinning, the solvent was dried to evaporate, yielding a sheath-core nanofiber membrane. The sheath-core nanofiber membrane was cut into a mask shape, and then a PEDOT:PSS conductive pattern was printed on it to obtain a soothing and anti-allergic functional layer, which was then laminated with a release film.

[0075] S2: The hydrophobic electron-donating layer of the assembled triboelectric layer is bonded to the side of the core-shell nanofiber membrane printed with a conductive pattern, ensuring that the conductive pattern is in contact with the hydrophobic electron-donating layer. Finally, conductive fibers are sewn onto the core-shell nanofiber membrane to connect the electron-accepting layer with the conductive pattern (ultimately, a circuit is formed between the electron-accepting layer, the hydrophobic electron-donating layer, and the conductive pattern), thus obtaining a microcurrent dry-state soothing and anti-allergic face mask.

[0076] Comparative Example 1 (using ordinary nanofiber membrane) The difference between this comparative example and Example 1 is that it does not use a core-sheath type nanofiber membrane.

[0077] The preparation process is as follows: S1: Preparation of the soothing and anti-allergic functional layer: Water-soluble silk fibroin, sorbitol, essential ingredients (hyaluronic acid, panthenol, xanthan gum, arginine), and a composition of soothing and anti-allergic plant extracts (chamomile water extract, poria cocos water extract, and purslane water extract in a mass ratio of 100:20:40) were added to water to prepare a spinning solution. The content of water-soluble silk fibroin was 12wt%, sorbitol was 2wt%, hyaluronic acid was 0.25wt%, panthenol was 0.25wt%, xanthan gum was 0.2wt%, arginine was 0.1wt%, and the composition of soothing and anti-allergic plant extracts was 1wt%. Electrospinning was performed using the spinning solution as raw material under the following conditions: spinning solution flow rate 0.008mm / s, temperature 35℃, relative humidity 45%; needle weight 18g, receiving distance 16cm, receiver rotation speed 1000rpm, and voltage 20kV. After electrospinning, the solvent was dried and evaporated to obtain a nanofiber membrane (50 micrometers). The nanofiber membrane was cut into the shape of a face mask, and then a PEDOT:PSS conductive pattern was printed on the nanofiber membrane to obtain a soothing and anti-allergic functional layer, which was then bonded to a release film.

[0078] S2: Same as Example 1.

[0079] Comparative Example 2 (Blank Face Mask) The difference between this comparative example and Example 1 is that the core layer of the core-sheath type nanofiber membrane does not contain soothing and anti-allergic plant extracts.

[0080] Face mask effect test (1) Human skin patch test Test substances: Mask samples from Example 1 and Comparative Example 1.

[0081] Participants: 15 women and 15 men. Age: 18-45 years. Health status: Participants had healthy skin and no history of skin diseases or allergies.

[0082] Patch method: The test mask sample was diluted with water and applied to the back of the volunteer, remaining on the skin for 48 hours. The mask sample was removed after 30 minutes, and the skin reaction was observed after the indentation disappeared. Observations were performed at 24 hours and 48 hours after the patch test.

[0083] The results were interpreted in accordance with the Cosmetic Hygiene Standards (2024 Edition). The results showed that none of the tested samples exhibited adverse reactions such as skin rashes, papules, or blisters. This indicates that the mask samples in all cases were mild and non-irritating.

[0084] (2) Histamine stimulation test Test samples: Mask samples from Example 1 and Comparative Examples 1-2 (cut to size 5×5cm) 2All samples were stored at room temperature for 3 months in advance (the purpose of which was to partially inactivate the active ingredients in the Comparative Example 1 sample).

[0085] Subject population: Healthy volunteers with significant allergic reactions to histamine (symptoms such as stinging, erythema, rash, and blisters) were selected as subjects using the histamine stimulation method. A total of 60 volunteers were selected, aged 18-45 years, with a male-to-female ratio of 1:1.

[0086] Test method: Select two 5×5cm points on the flexor side of the forearm. 2 The test site was kept dry. 50 μL of histamine (1.0 wt%) was applied to the test site and spread evenly. After 15 minutes, once symptoms such as erythema, rash, blisters, and stinging appeared, the mask sample was immediately moistened with 5 mL of water and applied to the test site, with gentle pressure applied (once every 2 seconds, for 180 seconds). Results were recorded after 30 minutes and observed and judged according to Table 5, the skin reaction evaluation criteria for the anti-allergic dermatitis test. Efficacy rate = (Number of excellent cases + Number of good cases) / Total number of cases × 100%.

[0087] Table 5: Criteria for Evaluating Skin Reactions in Anti-allergic Dermatitis Tests The test results are shown in Table 6.

[0088] Table 6: Results of the histamine stimulation test As shown in Table 6, Comparative Example 2, a blank mask sample without active ingredients, had an antihistamine irritation effect of only 40%. The sample in Example 1 had a significant soothing and anti-allergic effect on histamine-induced irritation, with an antihistamine irritation effectiveness rate of 95% after use by volunteers. The ordinary nanofiber membrane prepared in Comparative Example 1 had an antihistamine irritation effect of 65%, which was better than Comparative Example 2 but significantly worse than Example 1. This indicates that the sample in Example 1 has a better anti-irritation effect, and that loading the soothing and anti-allergic fabric extract into the core layer of the nanofiber membrane significantly improved its activity and stability.

[0089] (3) Human relaxation test Test samples: Mask samples from Example 1 and Comparative Examples 1-2 (cut to size 5×5cm) 2 All samples were stored at room temperature for 3 months in advance (the purpose of which was to partially inactivate the active ingredients in the Comparative Example 1 sample).

[0090] Subject population: Sixty healthy subjects aged 20-50 years were selected. Volunteers with obvious erythema on their cheeks, nose and other areas at rest were randomly divided into 3 groups.

[0091] The test period was 28 days. Data was collected from subjects after 28 consecutive days of use in a temperature and humidity controlled room. During the test, the product was used twice a week, and other soothing hair care products were not allowed to be used simultaneously. On days 0 and 28 of use, researchers collected data on the skin's stratum corneum moisture content, transepidermal water loss rate, and skin red pigment content. The results are shown in Table 7.

[0092] Table 7: Changes in skin measurement indicators before and after product use The data comparison in Table 7 shows that after 28 days of continuous use of the sample from Example 1, the stratum corneum moisture content of the skin was significantly increased, transepidermal water loss (TWEL) was significantly reduced, and the skin barrier function was also significantly repaired. In contrast, the effects on subjects using the samples from Comparative Example 1 and Comparative Example 2 were not significant. Subjects who used the sample from Example 1 for 28 days further underwent facial redness area images acquired via Visia-CR, as shown... Figure 8 As shown, a significant decrease in facial redness was observed in the subjects.

Claims

1. A microcurrent dry-state soothing and anti-allergic facial mask, characterized in that: It includes a soothing and anti-allergic functional layer and a triboelectric layer; the soothing and anti-allergic functional layer faces the skin during use, and the triboelectric layer is located in the area of ​​the cheek corresponding to the soothing and anti-allergic functional layer, wherein: The soothing and anti-allergy functional layer includes a core-sheath nanofiber membrane and conductive patterns distributed on the core-sheath nanofiber membrane; The sheath-core type nanofiber membrane has a sheath layer of water-soluble silk fibroin loaded with sorbitol and essential ingredients, and a core layer of water-soluble silk fibroin loaded with a composition of soothing and anti-allergic plant extracts. The soothing and anti-allergic plant extract composition includes chamomile extract, poria cocos extract, and purslane extract; The triboelectric layer comprises a hydrophobic electron donor layer, an elastic spacer layer, and an electron acceptor layer bonded together in sequence; the hydrophobic electron donor layer is bonded to a core-shell nanofiber membrane; and the hydrophobic electron donor layer and the electron acceptor layer are electrically connected to a conductive pattern, respectively.

2. The dry-state soothing and anti-allergic facial mask as described in claim 1, characterized in that: The Poria cocos extract and Portulaca oleracea extract are water extracts or alcohol-water extracts.

3. The dry-state soothing and anti-allergic facial mask as described in claim 1, characterized in that: The hydrophobic electron-donating layer is a hydrophobic polytetrafluoroethylene film with a conductive layer on the skin-facing side; The elastic spacer layer is an elastic porous silicone layer or a polyurethane porous sponge layer with through holes; The electron acceptor layer is a nylon layer with a conductive metal plated on its surface.

4. The dry-state soothing and anti-allergic facial mask as described in claim 1 or 3, characterized in that: The thickness of the soothing and anti-allergic functional layer is 40-60 micrometers; The thickness of the hydrophobic electron-donating layer is 20-30 micrometers; The thickness of the elastic spacer layer is 40-60 micrometers; The thickness of the electron acceptor layer is 20-30 micrometers.

5. The dry-state soothing and anti-allergic facial mask as described in claim 3, characterized in that: The conductive pattern is attached to the skin-facing side of the core-shell nanofiber membrane by printing. The conductive layer in the hydrophobic electron-donating layer is attached to the skin-facing side of the hydrophobic polytetrafluoroethylene film by printing.

6. A method for preparing a dry-state soothing and anti-allergic facial mask as described in any one of claims 1-5, characterized in that: Includes the following steps: S1: Preparation of the soothing and anti-allergic functional layer: Water-soluble silk fibroin, sorbitol, and essence ingredients are added to water to obtain the skin layer spinning solution; a combination of water-soluble silk fibroin and soothing and anti-allergic plant extracts is added to water to obtain the core layer spinning solution; a skin-core type nanofiber membrane is prepared by coaxial electrospinning technology, the solvent is dried and evaporated, the shape is cut, and then conductive patterns are printed on the skin-core type nanofiber membrane to obtain the soothing and anti-allergic functional layer; S2: The hydrophobic electron-donating layer of the assembled triboelectric layer is bonded to the side of the core-shell nanofiber membrane printed with conductive patterns, ensuring that the conductive patterns are in contact with the hydrophobic electron-donating layer. Finally, the electron-accepting layer is connected to the conductive patterns by sewing conductive fibers to obtain a microcurrent dry-state soothing and anti-allergic mask.

7. The dry-state soothing and anti-allergic facial mask as described in claim 6, characterized in that: In S1, the mass ratio of chamomile extract, poria cocos extract and purslane extract in the soothing and anti-allergic plant extract composition is 100:(10-30):(30-50).

8. The preparation method according to claim 7, characterized in that: In S1, The dermal spinning solution contains 10-15 wt% water-soluble silk fibroin and 1-3 wt% sorbitol; the total content of the essential components is 0.1-3 wt%. The core spinning solution contains 6-8 wt% water-soluble silk fibroin and 0.5-1.5 wt% soothing and anti-allergic plant extract composition.

9. The preparation method according to claim 6, 7, or 8, characterized in that: The essence ingredients include one or more of moisturizers, thickeners, and pH adjusters.

10. The preparation method according to claim 6, 7, or 8, characterized in that: In S1, the conditions for coaxial electrospinning are as follows: the flow rate of the sheath spinning solution is 0.005-0.01 mm / s, the flow rate ratio of the sheath spinning solution to the core spinning solution is 5-10:1; the temperature is 25-40℃, the relative humidity is 40-50%; the sheath needle is 15-20g, the core needle is 20-25g, the receiving distance is 15-18 cm, the receiver rotation speed is 500-1500 rpm, and the voltage is 18-22 kV.

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