Macro lens for nucleic acid detection
By designing a macro lens that combines multiple lenses and a corner prism, the problem of insufficient fluorescence receiving capability was solved, achieving efficient and high-definition fluorescence imaging and a compact device layout.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-02-10
- Publication Date
- 2026-04-07
AI Technical Summary
Existing macro lenses have limited fluorescence receiving capabilities in nucleic acid detection, and their small object-space numerical aperture and long working distance result in poor fluorescence imaging effects.
Design a lens group comprising a combination of multiple lenses, using high and low dispersion materials to correct chromatic aberration and aberrations, and achieving optical path deflection and efficient collection of fluorescence signals through the cooperation of corner prisms and filter wheels, and combining it with an sCMOS sensor for imaging.
It achieves high-resolution fluorescence imaging over a wide spectral range, improves fluorescence signal collection efficiency and imaging sensitivity, and has a compact spatial layout.
Smart Images
Figure CN121806253A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of molecular detection, and particularly relates to a micro-lens for nucleic acid detection. BACKGROUND
[0002] PCR technology is a molecular biology technology for amplifying specific DNA sequences outside the organism, and the PCR technology is widely applied to molecular biology detection and analysis due to its high sensitivity, strong specificity, high accuracy, repeatability and wide applicability.
[0003] At present, the PCR technology has become the "gold standard" in the fields of molecular biology and medical diagnosis, and the technology is combined with nucleic acid rapid extraction technology and microfluidic card integration to form a POCT device, which is more convenient for medical personnel to detect and takes less time. In the future, POCT will accelerate towards the goal of "15-minute rapid detection". The innovative amplification technology represented by the counter-flow PCR, combined with microfluidic and nucleic acid rapid extraction technology, is a very potential technical path for promoting POCT devices to realize ultra-portable, fully automatic and high-precision.
[0004] Although the integrated POCT device prefers a special optical detection module with high integration, rapidness and low cost, such as a rotating disc type or a single-direction back-and-forth scanning type, the fluorescence microscopic imaging method still has its unique advantages, which can be used for digital PCR (dPCR) and is the basis of fluorescence in situ hybridization (FISH) technology, and in the case of annular counter-flow PCR combined with spatial multiplexing, even one fluorescence channel can realize tens or even hundreds of detections.
[0005] The fluorescence microscopic imaging method usually adopts an image-side telecentric microscope objective combined with a field lens, and in the integrated detection device such as POCT, a long working distance objective or a micro-lens is used, which is more economical and compact. Generally, the micro-lens has a small object-side numerical aperture, and the NA is less than 0.1, and the working distance is relatively long, so the fluorescence receiving capacity is limited. Some object-side telecentric lenses have a large outer diameter near the object side. SUMMARY
[0006] In view of the defects of the prior art, the present application provides a micro-lens for nucleic acid detection to solve the problems in the background art.
[0007] In order to achieve the above object, the present application is implemented by the following technical scheme: A micro lens for nucleic acid detection, comprising a lens group, the lens group comprises a lens barrel, the right side of the lens barrel is communicated with a corner prism, the top of the corner prism is provided with a filter wheel, the inside of the lens barrel is fixedly provided with a first double convex lens, the right side of the first double convex lens is provided with a first double cemented lens, the first double cemented lens comprises a second double convex lens fixedly arranged in the inside of the lens barrel, the right side of the second double convex lens is fixedly connected with a third double concave lens, the right side of the first double cemented lens is provided with a second double cemented lens, the second double cemented lens comprises a fourth meniscus lens fixedly arranged in the inside of the lens barrel, the right side of the fourth meniscus lens is fixedly connected with a fifth meniscus lens, the right side of the second double cemented lens is provided with a third double cemented lens, the third double cemented lens comprises a sixth double concave lens fixedly arranged in the inside of the lens barrel, the right side of the sixth double concave lens is fixedly connected with a seventh double convex lens, the right side of the third double cemented lens is provided with a fourth double cemented lens, the fourth double cemented lens comprises an eighth double concave lens fixedly arranged in the inside of the lens barrel, the right side of the eighth double concave lens is fixedly connected with a ninth double convex lens, the right side of the fourth double cemented lens is provided with a fifth double cemented lens, the fifth double cemented lens comprises a tenth meniscus lens fixedly arranged in the inside of the lens barrel, and the right side of the tenth meniscus lens is fixedly connected with an eleventh double convex lens.
[0008] Preferably, the filter wheel comprises a cover fixedly connected to the top of the corner prism, a fixed plate is arranged in the cover, the fixed plate is provided with a through hole, and a filter plate is fixedly connected to the fixed plate through the through hole.
[0009] Preferably, the filter plate is fixedly connected with a gear ring outside, a drive worm is rotationally connected in the inside of the cover through a motor, and the outer surface of the drive worm is in mesh with the outer surface of the gear ring.
[0010] Preferably, the filter plate is one of a single band-pass filter and a multi-pass filter, and the number of the filter plates is greater than or equal to one.
[0011] Preferably, the corner prism is one of a right-angle prism, a reflector, a dichroic beam splitter and a stereoscopic beam splitter.
[0012] Preferably, an excitation light source module is arranged on the right side of the corner prism.
[0013] Preferably, a diaphragm is arranged between the ninth double convex lens and the tenth meniscus lens.
[0014] Preferably, a camera is communicated with the top of the cover, the camera is loaded with an sCMOS sensor, and a control circuit board is fixedly connected in the inside of the cover.
[0015] The application provides a micro-lens for nucleic acid detection. 1. The micro-lens for nucleic acid detection, by setting the lens group composed of multiple lenses, using the cementation of the second double convex lens and the third double concave lens in the first double cementation lens and the cementation of the eighth double concave lens and the ninth double convex lens in the fourth double cementation lens, high and low dispersion material pairing is used to correct chromatic aberration and high-order aberration, the cementation of the tenth meniscus lens and the eleventh double convex lens in the fifth double cementation lens is used to further suppress chromatic aberration and correct monochromatic aberration in a wide spectral range, and full-field, full-spectral high-definition fluorescence imaging effect is realized.
[0016] 2. The micro-lens for nucleic acid detection, by setting the lens group structure containing a diaphragm, using the cooperation of the nine lenses arranged in front of the diaphragm and the two lenses arranged behind the diaphragm, a large object numerical aperture and a long back focal length are realized, and high-efficiency collection and high-sensitivity detection of weak fluorescence signals are realized.
[0017] 3. The micro-lens for nucleic acid detection, by setting the corner prism between the lens barrel and the filter wheel, using the spatial connection and cooperation of the lens group, the corner prism, the filter wheel and the camera, a ninety-degree turn of the imaging light path is realized, space is provided for arranging the excitation light source module on the side surface of the corner prism, and the compactness of the spatial layout of the entire detection device is realized. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 The figure is the light path diagram inside the lens group of the application; Figure 2 The figure is the overall external structure diagram of the application; Figure 3 The figure is the overall internal structure diagram of the application; Figure 4 The figure is the internal structure diagram of the lens group of the application.
[0019] In the figure: 1, lens group; 11, lens barrel; 12, first double convex lens; 13, first double cementation lens; 131, second double convex lens; 132, third double concave lens; 14, second double cementation lens; 141, fourth meniscus lens; 142, fifth meniscus lens; 15, third double cementation lens; 151, sixth double concave lens; 152, seventh double convex lens; 16, fourth double cementation lens; 161, eighth double concave lens; 162, ninth double convex lens; 17, fifth double cementation lens; 171, tenth meniscus lens; 172, eleventh double convex lens; 2, corner prism; 3, filter wheel; 31, cover; 32, fixed plate; 33, filter; 34, gear ring; 35, driving worm; 4, camera; 5, control circuit board. DETAILED DESCRIPTION
[0020] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.
[0021] Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the invention, and should not be construed as limiting the invention.
[0022] Please see Figures 1-4 This invention provides a technical solution: a macro lens for nucleic acid detection, comprising a lens group 1, the lens group 1 including a lens barrel 11, a first biconvex lens 12 fixedly disposed inside the lens barrel 11, a first cemented doublet lens 13 disposed to the right of the first biconvex lens 12, the first cemented doublet lens 13 including a second biconvex lens 131 fixedly disposed inside the lens barrel 11, a third biconcave lens 132 fixedly connected to the right of the second biconvex lens 131, a second cemented doublet lens 14 disposed to the right of the first cemented doublet lens 13, the second cemented doublet lens 14 including a fourth meniscus lens 141 fixedly disposed inside the lens barrel 11, a fifth meniscus lens 142 fixedly connected to the right of the fourth meniscus lens 141, and a third cemented doublet lens 15 disposed to the right of the second cemented doublet lens 14. The third cemented doublet 15 includes a sixth biconcave lens 151 fixedly disposed inside the lens barrel 11. A seventh biconvex lens 152 is fixedly connected to the right side of the sixth biconcave lens 151. A fourth cemented doublet 16 is disposed to the right side of the third cemented doublet 15. The fourth cemented doublet 16 includes an eighth biconcave lens 161 fixedly disposed inside the lens barrel 11. A ninth biconvex lens 162 is fixedly connected to the right side of the eighth biconcave lens 161. A fifth cemented doublet 17 is disposed to the right side of the fourth cemented doublet 16. The fifth cemented doublet 17 includes a tenth meniscus lens 171 fixedly disposed inside the lens barrel 11. An eleventh biconvex lens 172 is fixedly connected to the right side of the tenth meniscus lens 171. An aperture is disposed between the ninth biconvex lens 162 and the tenth meniscus lens 171. The second biconvex lens 131 and the ninth biconvex lens 162 are both crown glass with high refractive index and low dispersion, and their Abbe number is greater than or equal to 65. The third biconcave lens 132 and the eighth biconcave lens 161 are both flint glass with high refractive index and high dispersion, and their refractive index is greater than or equal to 1.75 and their Abbe number is less than or equal to 45. The tenth meniscus lens 171 and the eleventh biconvex lens 172 are both crown glass. A corner prism 2 is connected to the right side of the lens barrel 11. The corner prism 2 is one of a right-angle prism, a reflecting mirror, a dichroic beam splitter, or a stereo beam splitter. An excitation light source module is set on the right side of the corner prism 2. A filter wheel 3 is set on the top of the corner prism 2. The filter wheel 3 includes a cover 31 fixedly connected to the top of the corner prism 2. A fixing plate 32 is set inside the cover 31. The fixing plate 32 has a through hole. A filter 33 is fixedly connected to the fixing plate 32 through the through hole. The filter 33 is one of a single bandpass filter or a multipass filter. The number of filters 33 is greater than or equal to one. A gear ring 34 is fixedly connected to the outside of the filter 33. A drive worm gear 35 is connected to the inside of the cover 31 through a motor. The outer surface of the drive worm gear 35 meshes with the outer surface of the gear ring 34. A camera 4 is connected to the top of the cover 31. The camera 4 is equipped with an sCMOS sensor. A control circuit board 5 is fixedly connected inside the cover 31. Table 1 is a detailed data sheet for an example macro lens; Table 1 The macro lens in Table 1 example has a focal length of 46.8mm, a fluorescence receiving spectrum range of 500~700nm, sufficient for fluorescence acquisition from four conventional channels: FAM, VIC, ROX, and CY5; an object-side numerical aperture (NA) of 0.15, with a telecentric object-side, a field of view of φ4.8mm, a magnification of 2.5X, a working distance between 35~36mm, and an MTF value ≥0.3 at an image-side resolution of 80 lp / mm. When paired with an sCMOS sensor with 2304×2304 effective pixels and each pixel size of 6.5μm×6.5μm, the object-side resolution can reach 2.6 micrometers.
[0023] In use, based on the principle of fluorescence microscopy, a sample is irradiated with excitation light of a specific wavelength, causing the fluorescent groups labeled in the sample to emit longer wavelength emission light. This weak emission light is then captured by lens group 1, reflected by corner prism 2, and filtered by filter wheel 3 to remove the excitation light and other stray light. Finally, a visible image is formed on camera 4. The algorithm processes these real-time images and then provides the results.
[0024] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.
Claims
1. A macro lens for nucleic acid detection, comprising a lens group (1), the lens group (1) comprising a lens barrel (11), a corner prism (2) connected to the right side of the lens barrel (11), and a filter wheel (3) disposed on the top of the corner prism (2), characterized in that: A first biconvex lens (12) is fixedly disposed inside the lens barrel (11). A first cemented doublet lens (13) is disposed to the right of the first biconvex lens (12). The first cemented doublet lens (13) includes a second biconvex lens (131) fixedly connected inside the lens barrel (11). A third biconcave lens (132) is fixedly connected to the right of the second biconvex lens (131). A second cemented doublet lens (14) is disposed to the right of the first cemented doublet lens (13). The second cemented doublet lens (14) includes a fourth meniscus lens (141) fixedly disposed inside the lens barrel (11). A fifth meniscus lens (142) is fixedly connected to the right of the fourth meniscus lens (141). A third cemented doublet lens (15) is disposed to the right of the second cemented doublet lens (14). (15) Includes a sixth biconcave lens (151) fixedly disposed inside the lens barrel (11), a seventh biconvex lens (152) fixedly connected to the right side of the sixth biconcave lens (151), a fourth biconvex lens (16) disposed to the right side of the third cemented doublet lens (15), the fourth cemented doublet lens (16) includes an eighth biconcave lens (161) fixedly disposed inside the lens barrel (11), a ninth biconvex lens (162) fixedly connected to the right side of the eighth biconcave lens (161), a fifth cemented doublet lens (17) disposed to the right side of the fourth cemented doublet lens (16), the fifth cemented doublet lens (17) includes a tenth meniscus lens (171) fixedly disposed inside the lens barrel (11), an eleventh biconvex lens (172) fixedly connected to the right side of the tenth meniscus lens (171).
2. A macro lens for nucleic acid detection according to claim 1, characterized in that: The filter wheel (3) includes a housing (31) fixedly connected to the top of the corner prism (2). A fixing plate (32) is provided inside the housing (31). The fixing plate (32) is provided with a through hole. A filter (33) is fixedly connected to the fixing plate (32) through the through hole.
3. A macro lens for nucleic acid detection according to claim 2, characterized in that: The filter (33) is fixedly connected to the outside of a gear ring (34), and the inside of the housing (31) is connected to a drive worm (35) via a motor. The outer surface of the drive worm (35) meshes with the outer surface of the gear ring (34).
4. A macro lens for nucleic acid detection according to claim 3, characterized in that: The filter (33) is one of a single bandpass filter or a multipass filter, and the number of the filter (33) is greater than or equal to one.
5. A macro lens for nucleic acid detection according to claim 1, characterized in that: The corner prism (2) is one of the following: right-angle prism, reflector, dichroic beam splitter, or stereo beam splitter.
6. A macro lens for nucleic acid detection according to claim 1, characterized in that: An excitation light source module is provided on the right side of the corner prism (2).
7. A macro lens for nucleic acid detection according to claim 1, characterized in that: The aperture is positioned between the ninth biconvex lens (162) and the tenth meniscus lens (171).
8. A macro lens for nucleic acid detection according to claim 2, characterized in that: The top of the housing (31) is connected to a camera (4), and a control circuit board (5) is fixedly connected inside the housing (31).