Cooling device for organs

Abstract

The invention relates to the technical field of organ preservation equipment, and provides a cooling device for organs, a cold storage pool of the cooling device is filled with a secondary refrigerant, and a refrigerator can refrigerate the secondary refrigerant in the cold storage pool. The storage box is soaked into the supercooled liquid bath pool, and organs are placed in the constant volume cavity of the supercooled liquid bath pool. And the circulating pipeline assembly forms a cold-carrying circulating loop between the cold storage pool and the supercooled liquid bath pool. Instantaneous output of large cooling capacity is achieved through the cold storage pool and the circulation pipeline assembly, and the problems that in a traditional cooling method, the speed is insufficient and the temperature difference between the inside and the outside is large are solved by combining a uniform and stable low-temperature environment formed by the supercooled liquid bath pool. Meanwhile, by arranging the constant-volume cavity, the volume expansion of the cryoprotectant during freezing is continuously inhibited in the cooling process, the supercooling degree of the cryoprotectant is remarkably improved, and ice crystal nucleation is effectively hindered, so that mechanical damage of ice crystals to cell structures is fundamentally avoided while rapid, uniform and deep cooling of organs is realized; and a key technology and equipment support is provided for profound hypothermia vitrification preservation of large-scale organs.

Description

Technical Field

[0001] This invention relates to the field of organ preservation equipment technology, and more particularly to a cooling device for organs. Background Technology

[0002] In the field of cryobiomedicine, particularly in organ transplantation and long-term preservation, achieving rapid, uniform, and deep cooling of large-scale biological organs (such as hearts, livers, and kidneys) is a key technological challenge. Currently, short-term preservation of organs after ex vivo typically relies on traditional cryoperfusion techniques, maintaining the organs in an ice-salt mixture or specialized preservation solution at 0–4°C. While this method can temporarily slow down cell metabolism, the preservation window is extremely short, usually not exceeding tens of hours, far from meeting the clinical needs for cross-regional allocation, organ matching, and long-term storage.

[0003] Currently, deep cryopreservation technology for large-scale organs mainly faces bottlenecks such as uneven cooling and low efficiency. Traditional methods have limited cooling output and slow response, resulting in significant temperature differences between the inside and outside of the organ. This not only makes it difficult to achieve overall vitrification but also easily causes tissue damage due to thermal stress. At the same time, these methods rely on slow heat conduction, resulting in low heat exchange efficiency. This causes organs to remain in the temperature range where ice crystals are easily formed for too long, leading to low preservation success rates. Summary of the Invention

[0004] To address the aforementioned technical problems, the present invention provides a cooling device for organs.

[0005] This invention provides a cooling device for organs, comprising: a cold storage tank containing a refrigerant; a refrigerator connected to the cold storage tank and used to cool the refrigerant in the cold storage tank; a supercooled liquid bath; a circulation pipeline assembly connected between the cold storage tank and the supercooled liquid bath to form a refrigerant circulation loop between the cold storage tank and the supercooled liquid bath; and a storage box immersed in the supercooled liquid bath, wherein the storage box has a fixed-volume cavity for placing organs and containing cryoprotectant.

[0006] According to the present invention, a cooling device for organs is provided, wherein the circulation pipeline assembly includes: a first pipeline connected between the outlet of the cold storage tank and the inlet of the supercooled liquid bath; a second pipeline connected between the outlet of the supercooled liquid bath and the inlet of the cold storage tank; and a first circulation pump connected to the first pipeline or the second pipeline and used to provide circulation power for the cooling circulation loop.

[0007] According to the present invention, a cooling device for organs further includes a temperature sensor for detecting the temperature within the constant volume chamber.

[0008] According to the present invention, an organ cooling device further includes a control device, which is connected to the temperature sensor and the first circulating pump, and is used to adjust the working state of the first circulating pump based on the detection result of the temperature sensor.

[0009] According to the present invention, an organ cooling device further includes: a self-circulating pipeline connected between the inlet and outlet of the supercooled liquid bath to form a self-circulating loop with the supercooled liquid bath, and the self-circulating pipeline being at least partially exposed to the external environment; and a second circulation pump disposed in the self-circulating pipeline and used to provide circulation power for the self-circulating loop.

[0010] According to the present invention, a cooling device for organs is provided, wherein the control device is connected to the second circulating pump and is used to control the working state of the second circulating pump based on the detection result of the temperature sensor.

[0011] According to the present invention, a cooling device for organs is provided, wherein the self-circulating pipeline is connected to the first pipeline via a three-way valve; the control device is connected to the three-way valve and is used to adjust the working state of the three-way valve based on the detection result of the temperature sensor.

[0012] According to the present invention, a cooling device for organs is provided, wherein the control device is configured to: calculate the real-time cooling rate of the constant volume chamber based on temperature data continuously acquired by the temperature sensor; compare the real-time cooling rate with a preset target cooling rate; and adjust the operating states of the first circulation pump, the second circulation pump and the three-way valve based on the comparison result, so that the real-time cooling rate tends to the target cooling rate.

[0013] According to the present invention, a cooling device for organs is provided, wherein the control device is configured to: acquire the target temperature value corresponding to the real-time temperature of the constant volume chamber and the preset target temperature curve based on the same time reference; calculate the instantaneous temperature difference between the real-time temperature and the corresponding target temperature value; and adjust the working state of the first circulation pump, the second circulation pump and the three-way valve according to the preset threshold range in which the instantaneous temperature difference is located.

[0014] According to the present invention, a cooling device for organs further includes a pressure detection device for detecting the pressure of the constant volume chamber.

[0015] The control device is connected to the pressure detection device, and the control device is configured to: when the pressure value detected by the pressure detection device exceeds a preset threshold, determine that the cooling process is abnormal and execute a safety strategy, the safety strategy including generating an alarm, cutting off the cooling circulation loop, and / or starting the self-circulation loop.

[0016] The organ cooling device provided by this invention includes a cold storage tank, a refrigerator, a subcooled liquid bath, a circulation pipeline assembly, and a storage box. The cold storage tank contains a refrigerant, and the refrigerator is connected to the cold storage tank and can cool the refrigerant in the cold storage tank. The storage box is immersed in the subcooled liquid bath and has a fixed-volume cavity for placing organs. The circulation pipeline assembly is connected between the cold storage tank and the subcooled liquid bath to form a refrigerant circulation loop between the two. The refrigerant can circulate between the cold storage tank and the subcooled liquid bath through the refrigerant circulation loop.

[0017] During the process, the refrigeration unit is first started to pre-cool and store the refrigerant in the cold storage tank. When organs need to be cryopreserved, the organs are sealed and placed in the constant-volume chamber of the storage box, and the circulation pipeline assembly is started to drive the low-temperature refrigerant to circulate between the cold storage tank and the supercooled liquid bath, thereby establishing a uniform deep low-temperature environment in the supercooled liquid bath. Immersed in this environment, the constant-volume chamber exchanges heat efficiently and uniformly with the internal organs through its chamber wall, achieving rapid cooling. At the same time, the closed and constant-volume characteristics of the constant-volume chamber continuously inhibit the volume expansion of the cryoprotectant when it freezes throughout the cooling process, providing double protection for the vitrification preservation of organs.

[0018] As described above, firstly, immersion in a supercooled liquid bath places the entire surface of the organ in a stable and uniform strongly cold environment, achieving comprehensive, synchronous, and efficient heat exchange. This effectively eliminates temperature gradients, avoids thermal stress damage, and creates crucial conditions for the uniform vitrification of large-scale organs. Secondly, the combination of precooling in a cold storage tank and forced convection in a circulating pipeline releases a large amount of cold energy in a short time, overcoming the bottlenecks of slow cold output and limited power in traditional refrigeration equipment. This allows the organ to quickly cross the dangerous temperature range for ice crystal formation, significantly improving the success rate of vitrification preservation. Simultaneously, a constant-volume chamber within the storage box provides a sealed space with a constant volume for the organ and cryoprotectant. Based on the phase change characteristics of water, under constant-volume conditions, the volume expansion of the aqueous solution upon freezing is mechanically inhibited, significantly increasing its supercooling and greatly hindering ice crystal nucleation and growth. This design, combined with efficient cooling, provides dual protection for the achievement of vitrification from both thermodynamic and physical perspectives. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0020] Figure 1 This is a simplified structural diagram of the organ cooling device provided by the present invention.

[0021] Reference numerals: 100, cold storage tank; 200, subcooled liquid bath; 300, storage box; 310, constant volume chamber; 410, first pipeline; 420, second pipeline; 430, first circulation pump; 510, self-circulating pipeline; 520, second circulation pump; 600, three-way valve; 700, pressure detection device. Detailed Implementation

[0022] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0023] In the description of the embodiments of the present invention, it should be noted that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0024] In the description of the embodiments of the present invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of the present invention based on the specific circumstances.

[0025] In embodiments of the present invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0026] In the description of this specification, the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate different embodiments or examples and features of different embodiments or examples described in this specification to make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer. The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0027] The following is combined with Figure 1 An organ cooling device provided in an embodiment of the present invention will be described. It should be understood that the following description is merely an illustrative embodiment of the present invention and does not constitute any particular limitation on the present invention.

[0028] Embodiments of the present invention provide a cooling device for organs, such as... Figure 1 As shown, it includes: a cold storage tank 100 containing a refrigerant; a refrigeration unit connected to the cold storage tank 100 and used to cool the refrigerant in the cold storage tank 100; a subcooled liquid bath 200; a circulation pipeline assembly connected between the cold storage tank 100 and the subcooled liquid bath 200 to form a refrigerant circulation loop between the cold storage tank 100 and the subcooled liquid bath 200; and a storage box 300 immersed in the subcooled liquid bath 200, and the storage box 300 is provided with a fixed-volume cavity 310 for placing organs and containing cryoprotectant.

[0029] The organ cooling device provided by this invention includes a cold storage tank 100, a refrigerator, a supercooled liquid bath 200, a circulation pipeline assembly, and a storage box 300. The cold storage tank 100 contains a refrigerant, and the refrigerator is connected to the cold storage tank 100, capable of cooling the refrigerant within the cold storage tank 100. The storage box 300 is immersed in the supercooled liquid bath 200, and a fixed-volume cavity 310 is provided within the storage box 300 for placing organs. The circulation pipeline assembly is connected between the cold storage tank 100 and the supercooled liquid bath 200 to form a refrigerant circulation loop between the two. The refrigerant can circulate between the cold storage tank 100 and the supercooled liquid bath 200 through this refrigerant circulation loop.

[0030] During operation, the refrigeration unit is first started to pre-cool and store the refrigerant in the cold storage tank 100. When organs need to be cryopreserved, the organs are sealed and placed in the constant-volume chamber 310 of the storage box 300, and the circulation pipeline assembly is started to drive the low-temperature refrigerant to circulate between the cold storage tank 100 and the supercooled liquid bath 200, thereby establishing a uniform deep low-temperature environment in the supercooled liquid bath 200. Immersed in this environment, the constant-volume chamber 310 conducts efficient and uniform heat exchange with the internal organs through its chamber wall, achieving rapid cooling. At the same time, the closed and constant-volume characteristics of the constant-volume chamber 310 continuously suppress the volume expansion of the cryoprotectant when it freezes throughout the cooling process, providing double protection for the vitrification preservation of organs.

[0031] As described above, firstly, immersion in the supercooled liquid bath 200 places the entire surface of the organ in a stable and uniform strongly cold environment, achieving comprehensive, synchronous, and efficient heat exchange. This effectively eliminates temperature gradients, avoids thermal stress damage, and creates crucial conditions for the uniform vitrification of large-scale organs. Secondly, the combination of precooling in the cold storage tank 100 and forced convection in the circulation pipeline releases a large amount of cold energy in a short time, overcoming the bottlenecks of slow cold output and limited power in traditional refrigeration equipment. This allows the organ to quickly cross the dangerous temperature range for ice crystal formation, significantly improving the success rate of vitrification preservation. Simultaneously, a constant-volume sealed space 310 is provided within the storage box 300 for the organ and cryoprotectant. Based on the phase change characteristics of water, under constant-volume conditions, the volume expansion of the aqueous solution upon freezing is mechanically inhibited, significantly increasing its supercooling and greatly hindering ice crystal nucleation and growth. This design, combined with efficient cooling, provides dual protection for the realization of the vitrification state from both thermodynamic and physical perspectives.

[0032] In one embodiment of the present invention, such as Figure 1As shown, the circulation piping assembly includes: a first pipe 410, which is connected between the outlet of the cold storage tank 100 and the inlet of the subcooled liquid bath 200; a second pipe 420, which is connected between the outlet of the subcooled liquid bath 200 and the inlet of the cold storage tank 100; and a first circulation pump 430, which is connected to the first pipe 410 or the second pipe 420 and is used to provide circulation power for the cooling circulation loop.

[0033] In one embodiment of the present invention, the organ cooling device further includes a temperature sensor for detecting the temperature within the constant volume chamber 310.

[0034] Furthermore, in one embodiment of the present invention, the organ cooling device further includes a control device, which is connected to the temperature sensor and the first circulation pump 430, and is used to adjust the working state of the first circulation pump 430 based on the detection result of the temperature sensor.

[0035] Specifically, for example, the first circulation pump 430 can be a low-temperature resistant magnetic centrifugal pump to ensure long-term stable operation and provide sufficient circulation power in a deep cryogenic environment; the temperature sensor can be a platinum resistance temperature sensor, with its probe directly inserted into the cryoprotectant inside the constant volume cavity 310 to accurately sense the actual temperature environment of the organ; the control device can be an embedded industrial computer system, which receives temperature signals through a data acquisition card and controls the motor speed of the first circulation pump 430 through a frequency converter.

[0036] During operation, the refrigeration unit first cools the refrigerant in the cold storage tank 100 to the target temperature. When the cooling program is initiated, the control device starts the first circulation pump 430, driving the low-temperature refrigerant to flow out of the cold storage tank 100, enter the subcooled liquid bath 200 through the first pipeline 410 for heat exchange, and then return to the cold storage tank 100 through the second pipeline 420, forming a closed loop. Simultaneously, the temperature sensor continuously monitors the temperature in the constant volume chamber 310 and transmits the data to the control device. The control device compares the real-time temperature with the preset cooling curve. If the actual temperature is higher than expected, the control device increases the speed of the first circulation pump 430 to accelerate the refrigerant circulation speed, thereby enhancing the heat exchange intensity; conversely, it decreases the speed to achieve precise adjustment of the cooling rate.

[0037] This technical solution constructs an active and controllable forced circulation cooling system. It overcomes system flow resistance through the first circulation pump 430, achieving high-flow-rate refrigerant circulation, significantly improving heat exchange efficiency, and ensuring rapid delivery of cooling capacity. Simultaneously, a closed-loop control mechanism based on real-time temperature feedback is introduced, enabling the device to move beyond simple cooling output and dynamically adjust cooling power according to the actual state of the organ. This achieves precise programmable control of the complex cooling process, effectively preventing temperature runaway and providing a stable and controllable low-temperature environment for large-scale organs.

[0038] In one embodiment of the present invention, such as Figure 1 As shown, the organ cooling device further includes: a self-circulating pipeline 510, which is connected between the inlet and outlet of the supercooled liquid bath 200 to form a self-circulating loop with the supercooled liquid bath 200, and the self-circulating pipeline 510 is at least partially exposed to the external environment; and a second circulation pump 520, which is provided in the self-circulating pipeline 510 and is used to provide circulation power for the self-circulating loop.

[0039] Furthermore, in one embodiment of the present invention, the control device is connected to the second circulation pump 520 and is used to control the working state of the second circulation pump 520 based on the detection result of the temperature sensor.

[0040] For example, the self-circulating pipeline 510 can be a coiled or serpentine metal pipe with a large area exposed to room temperature air to enhance heat exchange with the environment; the second circulation pump 520 is also a low-temperature pump; the control device manages the start-up, shutdown and speed of the second circulation pump 520 through an independent control loop or the same frequency converter.

[0041] During operation, when rapid cooling is required, the control device operates only the first circulation pump 430, enabling the low-temperature refrigerant in the cold storage tank 100 to powerfully cool the subcooled liquid bath 200. When the temperature sensor detects that the cooling rate in the constant volume chamber 310 is too fast, or when a gentle cooling plateau is needed, the control device can activate the second circulation pump 520 and close the passage to the cold storage tank 100, causing the refrigerant in the subcooled liquid bath 200 to flow only in the self-circulation loop. At this time, the refrigerant flows through the piping sections exposed to room temperature, absorbing heat from the environment, thereby slowing down its cooling rate.

[0042] This technical solution adds a powerful cooling rate regulation capability to the device. The self-circulating loop, acting as a controllable "heat source," actively and smoothly reduces the system's net cooling power by introducing ambient heat. This allows the device to flexibly execute complex cooling programs, such as actively slowing down when traversing specific temperature ranges prone to ice crystal formation, or precisely buffering before reaching the target storage temperature, greatly improving process control precision and organ survival rates.

[0043] In one embodiment of the present invention, such as Figure 1 As shown, the self-circulation pipeline 510 is connected to the first pipeline 410 via a three-way valve 600. The control device is connected to the three-way valve 600 and is used to adjust the operating state of the three-way valve 600 based on the detection results of the temperature sensor.

[0044] For example, the three-way valve 600 can be a solenoid valve, with one inlet connected to the incoming flow of the cold storage tank 100, the other inlet connected to the return flow of the self-circulation loop, and the outlet connected to the inlet of the subcooled liquid bath 200. The control device precisely controls the valve core position of the three-way solenoid valve by sending an electrical signal, so as to close or open the three-way solenoid valve. In the open state, its opening degree can also be adjusted.

[0045] During operation, the control device adjusts the mixing ratio at the two inlets of the three-way valve 600 to achieve stepless regulation of the refrigerant flow path. When maximum cooling power is required, the valve core fully connects the pipeline from the cold storage tank 100; when cooling needs to be slowed down, the valve core partially or fully connects the self-circulation loop, mixing or completely replacing the low-temperature refrigerant that has already exchanged heat with the environment with the refrigerant and delivering it to the liquid bath. This regulation is continuous and rapid, enabling a smooth transition in the cooling rate.

[0046] This structural design achieves integrated and precise flow path switching and flow distribution. The use of the three-way valve 600 avoids the control complexity and sudden flow changes caused by switching multiple valves, enabling continuous and linear adjustment of cooling intensity through a single actuator. This further improves the stability and response speed of temperature control, allowing the cooling curve to more perfectly match the preset ideal path.

[0047] In one embodiment of the present invention, the control device is configured to: calculate the real-time cooling rate of the constant volume chamber 310 based on the temperature data continuously acquired by the temperature sensor; compare the real-time cooling rate with a preset target cooling rate; and adjust the working state of the first circulation pump 430, the second circulation pump 520 and the three-way valve 600 based on the comparison result so that the real-time cooling rate tends to the target cooling rate.

[0048] For example, the control device has pre-stored target cooling rate curves optimized for different organ types, which specify the desired cooling rate within each temperature range. The control device can calculate the current actual cooling rate in real time.

[0049] During operation, the control device continuously executes a closed-loop control cycle of "measurement-calculation-comparison-adjustment". It first acquires the actual cooling rate at the current moment and then compares it with the set value of the target curve at that moment. If the actual rate is lower than the target, the control device increases the speed of the first circulation pump 430 and simultaneously adjusts the three-way valve 600 to increase the proportion of low-temperature refrigerant from the cold storage tank 100. If the actual rate is higher than the target, it may decrease the speed of the first circulation pump 430 and increase the proportion of the self-circulation loop in the three-way valve 600, or even start the second circulation pump 520 to introduce more ambient heat, thereby reducing the cooling intensity.

[0050] This technical solution elevates the control target from a single temperature to the more fundamental rate of temperature change, i.e., the cooling rate. It directly ensures that the thermal processes experienced by the organ meet the requirements for vitrification preservation, minimizing the risk of ice crystal formation or thermal stress damage caused by inappropriate rates.

[0051] In one embodiment of the present invention, the control device is configured to: acquire the real-time temperature of the constant volume chamber 310 and the target temperature value corresponding to the preset target temperature curve based on the same time reference; calculate the instantaneous temperature difference between the real-time temperature and the corresponding target temperature value; and adjust the working state of the first circulation pump 430, the second circulation pump 520 and the three-way valve 600 according to the preset threshold range of the instantaneous temperature difference.

[0052] Assume the preset target temperature curve is a temperature-time function curve that varies with time. The control device is equipped with a high-precision clock to ensure that real-time temperature sampling and target curve query are synchronized. At the same time, the control program sets multiple temperature difference threshold ranges, such as "positive large temperature difference", "positive small temperature difference", "negative small temperature difference", and "negative large temperature difference".

[0053] During operation, the control device reads the target temperature value at the current timestamp in each control cycle and calculates the difference between it and the real-time temperature (instantaneous temperature difference). Depending on which preset range this difference falls into, the controller triggers a corresponding preset control strategy. For example, when in the "positive large temperature difference" range (actual temperature much higher than the target), the controller executes the most powerful cooling strategy (e.g., the three-way valve 600 fully opens the cold storage tank 100, and the first circulation pump 430 operates at its highest speed); when in the "negative small temperature difference" range (actual temperature slightly lower than the target), a mild heating strategy is executed (e.g., activating self-circulation, or even briefly introducing ambient heat for a recovery).

[0054] Therefore, by designing control actions under different temperature difference thresholds, the system can quickly respond to temperature deviations and take corrective measures of appropriate strength, thereby ensuring that the actual cooling trajectory closely follows the preset ideal curve and ensuring process repeatability.

[0055] In one embodiment of the present invention, the organ cooling device further includes a pressure detection device 700, which is used to detect the pressure of the constant volume chamber 310.

[0056] The control device is connected to the pressure detection device 700. The control device is configured to: determine that the cooling process is abnormal and execute a safety strategy when the pressure value detected by the pressure detection device 700 exceeds a preset threshold. The safety strategy includes generating an alarm, cutting off the cooling circulation loop, and / or starting the self-circulation loop.

[0057] Specifically, for example, the pressure detection device 700 can be a pressure sensor installed on the wall of the constant volume chamber 310, capable of converting the physical pressure signal within the constant volume chamber 310 into an electrical signal and transmitting it to the control device in real time. The control device has a set pressure safety threshold, which is higher than the pressure fluctuation range caused by thermal expansion and contraction during normal cooling.

[0058] During operation, the pressure sensor continuously monitors the pressure within the constant-volume chamber 310. Ideally, the pressure should remain relatively stable during vitrification. If the refrigerant inside the chamber begins to freeze, its volume will expand due to the lower density of ice compared to water, causing a sharp increase in pressure within the sealed space. Once the control device detects that the pressure exceeds a preset safety threshold, it immediately determines that the vitrification process may have failed and executes several safety strategies: simultaneously notifying the operator with an audible and visual alarm, it can immediately stop the first circulation pump 430 to cut off the main cold source, and may simultaneously start the second circulation pump 520 and switch the three-way valve 600 to the self-circulation loop, using ambient heat to introduce positive thermal equilibrium into the system in an attempt to prevent further deterioration of the freezing process.

[0059] As described above, this technical solution adds a safety barrier to the entire cooling device. It utilizes the physical principle that freezing under constant volume conditions inevitably leads to an increase in pressure, using pressure as a key indirect indicator of successful vitrification. This safety mechanism enables early detection and intervention in the early stages of irreversible ice crystal damage to tissues, achieving an upgrade from simple temperature control to process state safety monitoring, greatly improving the reliability and success rate of the entire organ preservation experiment. Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An organ cooling device, characterized by, The organ cooling device comprises: a cold storage tank (100) in which a coolant is stored; a refrigerating machine connected to the cold storage tank (100) and configured to cool the coolant in the cold storage tank (100); a super-cooled liquid bath tank (200); a circulating pipeline assembly connected between the cold storage tank (100) and the super-cooled liquid bath tank (200) to form a coolant circulation loop between the cold storage tank (100) and the super-cooled liquid bath tank (200); a storage box (300) immersed in the super-cooled liquid bath tank (200), and the storage box (300) is provided with a constant volume cavity (310) for placing an organ and storing a cryoprotective agent.

2. The organ cooling device of claim 1, wherein, The circulating pipeline assembly comprises: a first pipeline (410) connected between an outlet of the cold storage tank (100) and an inlet of the super-cooled liquid bath tank (200); a second pipeline (420) connected between an outlet of the super-cooled liquid bath tank (200) and an inlet of the cold storage tank (100); a first circulating pump (430) connected to the first pipeline (410) or the second pipeline (420) and configured to provide circulating power for the coolant circulation loop.

3. The organ cooling device of claim 2, wherein, The organ cooling device further comprises: a temperature sensor configured to detect a temperature in the constant volume cavity (310).

4. The organ cooling device of claim 3, wherein, The organ cooling device further comprises: a control device connected to the temperature sensor and the first circulating pump (430) and configured to adjust an operating state of the first circulating pump (430) based on a detection result of the temperature sensor.

5. The organ cooling device of claim 4, wherein, The organ cooling device further comprises: a self-circulating pipeline (510) connected between the inlet and the outlet of the super-cooled liquid bath tank (200) to form a self-circulation loop with the super-cooled liquid bath tank (200), and the self-circulating pipeline (510) is at least partially exposed to an external environment; a second circulating pump (520) provided in the self-circulating pipeline (510) and configured to provide circulating power for the self-circulation loop.

6. The organ cooling device of claim 5, wherein, The control device is connected to the second circulating pump (520) and configured to control an operating state of the second circulating pump (520) based on the detection result of the temperature sensor.

7. The organ cooling device of claim 6, wherein, The self-circulating pipeline (510) is connected to the first pipeline (410) through a three-way valve (600). The control device is connected to the three-way valve (600) and configured to adjust an operating state of the three-way valve (600) based on the detection result of the temperature sensor.

8. The organ cooling device of claim 7, wherein, The control device is configured to: calculate a real-time cooling rate of the constant volume cavity (310) based on temperature data continuously acquired by the temperature sensor; compare the real-time cooling rate with a preset target cooling rate; and Based on the comparison result, the working states of the first circulating pump (430), the second circulating pump (520) and the three-way valve (600) are adjusted to make the real-time cooling rate tend to the target cooling rate.

9. The organ cooling device of claim 7, wherein, The control device is configured to: acquire, based on the same time reference, a real-time temperature of the constant-volume cavity (310) and a corresponding target temperature value in a preset target temperature curve; calculate an instantaneous temperature difference between the real-time temperature and the corresponding target temperature value; adjust the working states of the first circulating pump (430), the second circulating pump (520) and the three-way valve (600) according to a preset threshold range in which the instantaneous temperature difference is located.

10. The organ cooling device of any of claims 5 to 9, wherein, The organ cooling device further comprises: a pressure detection device (700) for detecting the pressure of the constant-volume cavity (310); The control device is connected with the pressure detection device (700), and the control device is configured to: when the pressure value detected by the pressure detection device (700) exceeds a preset threshold value, it is determined that the cooling process is abnormal and a safety strategy is executed, the safety strategy including generating an alarm, simultaneously cutting off the cooling load circulating loop, and / or starting the self-circulating loop.

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