Plant growth regulator

Abstract

The invention relates to a plant growth regulator, in particular to a paclobutrazol and choline chloride mixed composition used for regulating crop growth, the regulator is mainly characterized in that the effective active ingredients of the regulator are paclobutrazol and choline chloride, auxiliaries and other excipients are added, and the dosage form of the regulator is a suspending agent. The regulator can obviously regulate the growth of peanuts, increase the pod number of plants, improve the hundred-kernel weight of the peanuts, has an obvious yield increasing effect, can also improve the quality of the peanuts and increase the content of fat, oleic acid and linoleic acid of the peanuts, has a comprehensive effect compared with a control agent, gives consideration to pod increase and hundred-kernel weight increase, is stable in yield increasing and improves the quality, is safe to the peanuts, and is worthy of popularization and application. And the economic benefit is effectively improved.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a plant growth regulator containing paclobutrazol, choline chloride, adjuvants, and other excipients. Background Technology

[0002] Paclobutrazol is a plant growth regulator that can delay plant growth, inhibit stem elongation, shorten internodes, promote tillering, increase plant stress resistance, and improve yield.

[0003] Choline chloride is a common plant growth regulator and is widely used in plant growth regulation.

[0004] During hot, rainy, and humid seasons, especially after peanuts flower and peg, when they enter the fruit growth and expansion stage, excessive rainfall leads to excessive vegetative growth of the above-ground stems and leaves. This causes excessive vegetative growth in most fields, particularly in highly fertile and water-rich areas, resulting in dense canopy cover, reduced net assimilation rate, and insufficient nutrition for the underground pods. This results in "flowering without fruiting," small and few peanut pods, and underdeveloped kernels, affecting both yield and quality. Furthermore, the plants are prone to lodging, leading to even greater yield reduction. Therefore, if excessive vegetative growth occurs after flowering and pegging (plant height exceeding 40cm from pegging to the early pod-forming stage), timely measures should be taken to control the excessive growth of above-ground stems and leaves, promote reproductive growth, and ensure more, fuller kernels and higher yields. The applicant's experiments have shown that mixing paclobutrazol with choline chloride, which has a different mechanism of action, can control peanut growth and development, thereby improving yield and quality. Summary of the Invention

[0005] The purpose of this invention is to provide a plant growth regulator that, when formulated with adjuvants and other excipients in a specific ratio, can effectively inhibit the vegetative growth of the above-ground parts, dwarf the plant, darken the leaf color, promote the enhancement of root vitality, and improve the transport and distribution of photosynthetic products, thus having a significant synergistic effect on increasing peanut yield and improving quality.

[0006] The technical solution of this invention is:

[0007] A plant growth regulator, wherein the effective active ingredients of the regulator are paclobutrazol and choline chloride, and adjuvants and other excipients are added to form a suspension, wherein the weight ratio of paclobutrazol to choline chloride is 1:2, and the remaining amount is the adjuvants and other excipients; the total weight of paclobutrazol and choline chloride in the regulator is 12% to 60%, preferably 45%.

[0008] Furthermore, the regulators, additives, and excipients are selected from one or any combination of dispersants, wetting agents, antifreeze agents, thickeners, stabilizers, defoamers, and fillers.

[0009] Furthermore, the regulator is used to regulate peanut growth, specifically to increase peanut yield and improve peanut quality, effectively increasing the fat content, oleic acid content and linoleic acid content of peanuts.

[0010] Furthermore, the regulator is used to adjust the dosage of effective components for peanut growth at 225-300 mg / kg.

[0011] The dispersant is selected from one or more of the following: alkyl naphthalene sulfonate, alkyl naphthalene sulfonate formaldehyde polymer, lignin sulfonate, polycarboxylate, and EO-PO block copolymer; the wetting agent is selected from one or more of the following: fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether (nonylphenol polyoxyethylene ether and octylphenol polyoxyethylene ether), fatty amine polyoxyethylene ether, alkyl glycoside, trisiloxane polyoxyethylene ether, polyarylphenol polyoxyethylene ether, and castor oil polyoxyethylene ether; the antifreeze agent is selected from one or more of the following: ethylene glycol, propylene glycol, glycerol, and urea; the thickener is selected from xanthate gum, polyvinyl alcohol, and carboxymethyl cellulose; the stabilizer is selected from one of the following: silica and magnesium aluminum silicate; the preservative is sodium benzoate; the defoamer is selected from one of the following: organosilicone, C10-20 saturated fatty acid compounds, and C8-10 fatty alcohols; and the filler is water.

[0012] The regulator comprises the following components: paclobutrazol 4%–20%, choline chloride 8%–40%, dispersant 2%–10%, wetting agent 2%–9%, ​​antifreeze 0%–6%, thickener 0.05%–2%, stabilizer 0%–2%, preservative 0%–2%, defoamer 0%–2%, and filler balance.

[0013] The advantages of this invention are: (1) Compared with single agents, it has a good synergistic effect on increasing peanut yield and improving quality; (2) It can effectively increase peanut yield, increase peanut fat content, oleic acid content and linoleic acid content, increase the economic value of peanut and increase farmers' income; (3) It is low in toxicity to humans and animals, safe to use, the product degrades quickly, has little environmental pollution, and the residue in the soil will not have an adverse effect on subsequent crops; (4) The plant growth regulator contains a thickener, which is conducive to crop absorption, resistant to rain erosion, and has a long-lasting effect. Detailed Implementation

[0014] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0015] Application Example 1

[0016] Example 1: 45% choline chloride·paclobutrazol suspension

[0017] By weight percentage, paclobutrazol 15%, choline chloride 30%, polycarboxylate 4%, fatty alcohol polyoxyethylene ether 2%, ethylene glycol 3%, xanthate gum 0.5%, sodium benzoate 0.8%, silicone defoamer 0.10%, and water to 100%.

[0018] Example 2: 45% choline chloride·paclobutrazol suspension

[0019] By weight percentage, paclobutrazol 15%, choline chloride 30%, alkylnaphthalene sulfonate 4%, trisiloxane polyoxyethylene ether 12%, propylene glycol 5%, polyvinyl alcohol 0.5%, magnesium aluminum silicate 0.5%, sodium benzoate 1%, C8-10 fatty alcohol 0.2%, and water to 100%.

[0020] Example 3: 45% choline chloride·paclobutrazol suspension

[0021] By weight percentage, paclobutrazol 15%, choline chloride 30%, alkyl naphthalene sulfonate formaldehyde polymer 2%, polyarylphenol polyoxyethylene ether 2%, glycerol 4%, xanthate gum 0.2%, sodium benzoate 1%, C10-20 saturated fatty acid compounds 0.3%, and water to 100%.

[0022] Example 4: 45% choline chloride·paclobutrazol suspension

[0023] By weight percentage, paclobutrazol 15%, choline chloride 30%, EO-PO block copolymer 2.5%, fatty alcohol polyoxyethylene ether 2%, alkylphenol polyoxyethylene ether 1%, ethylene glycol 5%, carboxymethyl cellulose 0.5%, silicone 0.8%, and water to 100%.

[0024] Example 5: 15% choline chloride·paclobutrazol suspension

[0025] By weight percentage, paclobutrazol 5%, choline chloride 10%, lignin sulfonate 3%, alkylphenol polyoxyethylene ether 4%, urea 2%, carboxymethyl cellulose 1%, silicone defoamer 1%, and water to 100%.

[0026] Example 6: 30% choline chloride·paclobutrazol suspension

[0027] By weight percentage, the ingredients are: 10% paclobutrazol, 20% choline chloride, 4% alkyl naphthalene sulfonate, 4% trisiloxane polyoxyethylene ether, 0.4% polyvinyl alcohol, 0.5% magnesium aluminum silicate, 1.5% sodium benzoate, 0.5% C8-10 fatty alcohol defoamer, and water to 100%.

[0028] Example 7: 60% choline chloride·paclobutrazol suspension

[0029] By weight percentage, paclobutrazol 20%, choline chloride 40%, EO-PO block copolymer 3%, polycarboxylate 1%, fatty alcohol polyoxyethylene ether 3%, alkyl glycoside 2%, glycerol 5%, xanthan gum 0.10%, silicone defoamer 1%, and water to 100%.

[0030] Implementation Example 2:

[0031] Report on the Indoor Combined Toxicity Assay of Choline Chloride and Paclobutrazol in Regulating Peanut Growth

[0032] Test target: peanut, variety Shanhua 8.

[0033] Experimental method: foliar spraying

[0034] Test basis: Refer to NY / T 2061.2-2011 and NY / T 2061.5-2016.

[0035] 1. Experimental Objective

[0036] Choline chloride and paclobutrazol were used as test agents, and peanuts at the budding stage were used as test subjects. The combined effect of the two agents was determined by foliar spraying in the laboratory to screen the optimal ratio and determine the formula. This provides a basis for formula screening for the rational mixing of the two agents and the development of effective agents for regulating peanut growth. The test results are reported as follows.

[0037] 2. Cultivation conditions

[0038] Peanuts are cultivated using the pot method.

[0039] 3 Experimental Design

[0040] 3.1 Reagents

[0041] 96% paclobutrazol technical grade, provided by the applicant;

[0042] The 98% choline chloride technical grade was provided by the applicant.

[0043] 3.2 Test Treatment

[0044] 3.2.1 Dosage setting

[0045] Based on the individual activity of the two agents and the characteristics of the laboratory tests, and on the basis of the preliminary test, the dosages of paclobutrazol and choline chloride were set to different combinations, as shown in Table 1, for a total of 15 groups.

[0046] An aqueous solution containing the same solvent and emulsifier but without any reagents was used as a blank control for in-house bioassays.

[0047] Table 1 Experimental reagent dosing design

[0048]

[0049] 3.2.2 Experimental Repetition

[0050] Each concentration of the experimental agent was divided into 4 replicates, with 20 pots per replicate and 1 peanut plant per pot.

[0051] 3.3 Processing Method

[0052] Apply the pesticide once at the end of the flowering period of potted peanuts. Follow the experimental design to treat from low to high doses. Measure the peanut plant height before and 20 days after the pesticide application.

[0053] 4. Data Survey and Statistical Analysis

[0054] 4.2 Data Statistical Analysis

[0055] The theoretical inhibition rate (growth rate) E0 (E0=X+YX*Y / 100) of each treatment combination was calculated using the Gowing method. Then, it was compared with the measured inhibition rate (growth rate) (E) to evaluate the combined effect of the two treatments on peanuts.

[0056] When the E-E0 value is greater than 10%, it indicates a synergistic effect; when the E-E0 value is less than -10%, it indicates an antagonistic effect; and when the E-E0 value is between -10% and 10%, it indicates an additive effect. The optimal ratio is determined based on factors such as the actual plant height, the characteristics of the two plant growth regulators, and the balance of the formulation.

[0057] In the formula, X is the plant height inhibition rate when the dosage of paclobutrazol is P; Y is the plant height inhibition rate when the dosage of choline chloride is Q.

[0058] 5 Results and Analysis

[0059] Table 2. Effects of choline chloride and paclobutrazol and their different ratios on peanut growth regulation.

[0060]

[0061] Table 2 shows that after mixing the experimental agents, the E-E0 value of the treatment with paclobutrazol 90 mg / L + choline chloride 180 mg / L was 10.76, indicating a synergistic effect. The E-E0 value of the treatment with paclobutrazol 60 mg / L + choline chloride 125 mg / L was 10.06, which is greater than 10%, also indicating a synergistic effect. This indicates that the paclobutrazol + choline chloride treatments of 90 mg / L + 180 mg / L and 60 mg / L + 125 mg / L were most effective in inhibiting plant height growth.

[0062] Observations during the experiment showed that the two agents and their respective dosage ratios were safe for the growth of the experimental peanuts and caused no phytotoxicity.

[0063] Application Example 3: Field Efficacy Experiment of the Product in Regulating Peanut Growth 1. Experimental Objective

[0064] To verify the regulatory effect, appropriate timing of application, recommended dosage, and safety of the 45% choline chloride-paclobutrazol suspension developed by the applicant on peanut growth, and to provide a basis for the registration of the product.

[0065] 2 Materials and Methods

[0066] Application method: spraying, water consumption 450-750 liters / hectare.

[0067] 2.1 Experimental reagent treatment

[0068] Table 3 Treatment of test reagents

[0069]

[0070] 2.2 Experimental subjects and crops

[0071] Experimental subject: peanut. The peanut seedlings grew well after emergence.

[0072] 2.3 Application Method

[0073] Application time: Spray once at the end of the peanut flowering period.

[0074] 2.4 Survey Time, Frequency, and Methods

[0075] A survey was conducted 7 days after application of the pesticide, before harvest, and after harvest, for a total of 3 surveys.

[0076] Seven days after application, investigate for any abnormalities such as deformity, wilting, chlorosis, or seedling death to evaluate the safety of the pesticide. Before harvest, take 10 consecutive samples from each plot for seed testing, measure the weight of 100 pods and kernels, and determine the yield of the plot. One month after peanut harvest, determine the protein content of the kernels using the Kjeldahl method.

[0077] 2.5 Method for Calculating Drug Efficacy

[0078] Increase rate (%) = [(treatment output - control output) / control output] × 100.

[0079] 3 Results and Analysis

[0080] Table 4. Effects of the test agents on peanut yield and quality. Test results.

[0081]

[0082]

[0083] 4. Conclusion

[0084] Suitable application time and dosage: The experimental agent was sprayed once at the end of the peanut flowering period. It can be seen that the 45% choline chloride·paclobutrazol suspension with an effective ingredient dosage of 225 mg / kg and 300 mg / kg has a good regulatory effect on peanut growth. It can inhibit excessive growth of peanut plants, promote the growth of peanut underground pods, significantly increase yield at harvest, and improve peanut quality. Compared with the control single agent and the blank control, it has a highly significant difference.

[0085] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. A plant growth regulator, characterized in that: The active ingredients of the regulator are paclobutrazol and choline chloride. With the addition of adjuvants and other excipients, a suspension is prepared, wherein the weight ratio of paclobutrazol to choline chloride is 1:2, and the remaining amount is the adjuvants and other excipients.

2. The regulator according to claim 1, characterized in that: The total weight of paclobutrazol and choline chloride in the regulator is 12% to 60%.

3. The regulator according to claim 2, characterized in that: The total weight of paclobutrazol and choline chloride in the regulator is 45%.

4. The regulator according to claim 1, characterized in that: The additives and excipients mentioned are selected from one or any combination of dispersants, wetting agents, antifreeze agents, thickeners, stabilizers, defoamers, and fillers.

5. The regulator according to any one of claims 1 to 4, characterized in that: The regulator is used to regulate peanut growth.

6. The regulator according to claim 5, characterized in that: The regulator is used to adjust the dosage of effective components for peanut growth at 225-300 mg / kg.

7. The regulator according to claim 5, characterized in that: The regulators mentioned above are used to increase peanut yield and improve peanut quality.

8. The regulator according to claim 7, characterized in that: The regulator is used to increase the fat content, oleic acid content and linoleic acid content of peanuts.

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