Storage method for improving active ingredients of lotus seedpod, product and application of product

Abstract

The invention discloses a storage method for improving active ingredients of lotus seedpods, a product and application of the product. Fresh lotus seedpod shells are cut up and then mixed with glucose to obtain a mixture silage, and the silage is dried to obtain the lotus seedpod particles with improved active ingredients. The silage is creatively used as a temporary storage means, the sugar silage is added into the lotus seedpod shells, so that the antioxidant activity and the antibacterial ability of the lotus seedpod shells can be improved, the high-quality silage can be obtained by adding the sugar silage or adding the dry lotus seedpod shell powder into the forage silage, the conversion of the lotus seedpod shells from wastes to high-value products is realized, and economic and environment-friendly benefits are achieved.

Description

Technical Field

[0001] This invention belongs to the field of agricultural and sideline product processing, and specifically relates to a storage method, product, and application of improving the active ingredients in lotus seedpod shells. Background Technology

[0002] Lotus seedpod shells are a major byproduct of lotus seed processing, rich in natural antioxidants such as polyphenols and flavonoids, and have high development and utilization value. However, currently, the vast majority of lotus seedpod shells are discarded or incinerated, causing resource waste and environmental pollution. The antioxidants in lotus seedpod shells are easily oxidized, undergo enzymatic reactions, or are decomposed by microorganisms during conventional storage, leading to a decrease or even loss of biological activity. Currently, common storage methods include natural drying, hot air drying, freeze drying, and sealed storage. However, natural drying is inefficient, prone to mold growth, and results in significant loss of active ingredients; hot air drying is energy-intensive, and high temperatures easily destroy heat-sensitive antioxidants; freeze drying, while effective, is extremely expensive and difficult to apply on a large scale; simple sealing cannot inhibit the enzymatic activity and slow oxidation reactions within the material itself. Therefore, finding a low-cost, high-efficiency method that can maximize the preservation and even enhance the active substances in lotus seedpod shells is crucial.

[0003] Silage is a technique for preserving wet materials through lactic acid bacteria fermentation. Due to its low cost and high effectiveness, it has been widely used for preserving forage and some fruit and vegetable byproducts. However, directly applying conventional silage technology to lotus seedpods has limitations. The high fiber content and insufficient water-soluble carbohydrates in lotus seedpods result in slow silage initiation, insufficient acid production, and difficulty in effectively inhibiting harmful microbial activity. The result is often poor silage quality, with significant decomposition of proteins and active substances, and decreased antioxidant activity. Currently, there is a lack of effective storage methods to preserve the antioxidant activity of lotus seedpods, and research on how to maximize their production capacity.

[0004] Therefore, it is necessary to develop a method that can systematically optimize the processing and storage of lotus seedpod shells, synergistically realize their high-value and resource utilization, determine their optimal storage process, improve the quality and functional activity of silage, expand their application in feed, and thus form a comprehensive solution. Summary of the Invention

[0005] The primary objective of this invention is to overcome the shortcomings of existing lotus seedpod drying technologies, such as large loss of active substances, high cost, or high energy consumption, and to provide a method for storing lotus seedpods with improved active ingredients.

[0006] Another object of the present invention is to provide the application of the above-described storage method in the processing of lotus seedpod shells.

[0007] Another object of the present invention is the product obtained by the above-described storage method.

[0008] Another object of the present invention is to provide applications of the above-described products.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: a method for storing lotus seedpod shells to enhance their active ingredients, comprising the following steps: (1) Chop fresh lotus seedpods and mix them with glucose to obtain a mixture; (2) The mixture is ensilized and then dried to obtain lotus seedpod shell granules with increased active ingredients.

[0010] The preferred degree of chopping in step (1) is to chop to 2-3 cm.

[0011] The amount of glucose used in step (1) is preferably calculated as 1 to 3% of the weight of the lotus seedpod shell; more preferably, it is calculated as 2% of the weight of the lotus seedpod shell.

[0012] The silage method described in step (2) is a conventional silage method. The preferred steps are as follows: seal the mixture and degas it.

[0013] The preferred silage time in step (2) is 15 to 60 days.

[0014] The drying method described in step (2) is preferably oven drying or freeze drying.

[0015] The above storage method is applied in the processing of lotus seedpod shells.

[0016] A product obtained through the above storage method is lotus seedpod shell granules with enhanced active ingredients.

[0017] The above products are used in the preparation of natural antioxidants and antibacterial agents.

[0018] The preferred application of lotus seedpod shells as a silage additive includes the following steps: adding dried lotus seedpod shell powder to the silage raw materials, mixing evenly, and then ensiling to obtain silage.

[0019] The lotus seedpod shell powder is granules obtained after drying lotus seedpod shells; preferably, it is granules obtained by drying or freeze-drying lotus seedpod shells; more preferably, it is lotus seedpod shell granules with enhanced active ingredients as described above.

[0020] The preferred raw material for silage is purple elephant grass.

[0021] Compared with the prior art, the present invention has the following beneficial effects: (1) This invention is the first to discover that silage, as a temporary storage method, can effectively retain the antioxidant activity of lotus seedpod shells after 30 days of storage.

[0022] (2) By using additives, this invention effectively solves the problem of poor fermentation of lotus seedpod shell silage. It not only greatly preserves its active substances, but also significantly improves its antioxidant and antibacterial activities compared with traditional methods and natural silage, thus realizing the transformation from "preservation" to "value-added".

[0023] (3) This invention has pioneered three application modes of lotus seedpod shells: First, as a high-quality unconventional feed source, adding 2% glucose to silage can significantly improve its fermentation quality and reduce breeding costs; second, as a natural silage feed additive, it can be used to improve the quality of other roughage and enhance the overall feed value; third, as a high-value functional extract raw material, it can be used to develop natural antioxidants and antibacterial agents, which greatly improves the utilization level and economic benefits of lotus seedpod shells.

[0024] (4) The entire process of this invention does not require expensive equipment. It takes biological fermentation as the core, has low cost, and is easy to scale up and promote. It provides a new idea for the full-scale and high-value utilization of agricultural waste. Attached Figure Description

[0025] Figure 1 The graph shows the results of total phenolic and total flavonoid content detection in lotus seedpod shells obtained from different storage methods (sun-drying, oven-drying, freeze-drying, silage-drying, and silage-freeze-drying); different letters in the graph indicate significant differences. P <0.05).

[0026] Figure 2 The antioxidant capacity (DPPH free radical scavenging capacity, ABTS scavenging capacity) of lotus seedpod shells obtained by different storage methods (sun-drying, oven-drying, freeze-drying, silage drying and silage freeze-drying) + The graph shows the results of the detection of free radical capacity and FRAP iron ion reducing capacity; different letters in the graph indicate significant differences. P <0.05).

[0027] Figure 3 The graph shows the results of total phenol and total flavonoid content detection in dried and freeze-dried lotus seed pod powder and silage powder with different additives and silage days; CK: control, CZ1 is Lactobacillus plantarum ( Lactobacillus plantarum CCZZ1; ZH1 is a type of Lactobacillus flavus ( Lactobacillus parafarraginis ZH1; G indicates the addition of 2% glucose; C indicates the addition of 0.02% cellulase; different letters are used to indicate significant differences between drying and freeze-drying in the figure. P <0.05).

[0028] Figure 4 Antioxidant capabilities (DPPH free radical scavenging ability, ABTS scavenging ability) of dried and freeze-dried lotus pod shell powder with different additives and silage days. +Free radical capacity and FRAP iron ion reducing capacity); CK: control, CZ1 is Lactobacillus plantarum CCZZ1 ( Lactobacillus plantarum ZH1 is a type of Lactobacillus flavus ZH1 ( Lactobacillus parafarraginis G represents the addition of 2% glucose; C represents the addition of 0.02% cellulase; different letters are used to indicate significant differences between drying and freeze-drying in the figure. P <0.05).

[0029] Figure 5 The graph shows the antibacterial effect of polyphenol extracts from lotus seedpods obtained from different treatments on Staphylococcus aureus.

[0030] Figure 6 The results of pH and lactic acid content on day 30 after adding different proportions of dried lotus seedpod powder to purple elephant grass silage are shown in the figure; different letters in the figure indicate significant differences. P <0.05). Detailed Implementation

[0031] The present invention will be further described in detail below with reference to the embodiments and accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0032] Example 1: Storage methods to enhance the antioxidant capacity of lotus seedpod shells The collected fresh lotus pods (provided by Hengyang Deyuan Lotus Industry Co., Ltd.) were chopped into pieces of 2-3 cm in length using a shredder and then mixed evenly. The resulting shredded sample was then processed as follows: (1) Sun drying: Place the broken sample in the sun to dry until constant weight, and obtain the sun-dried sample.

[0033] (2) Drying: Place the crushed sample in an oven at 70°C and dry it to constant weight to obtain a dried sample.

[0034] (3) Freeze-drying: Pre-freeze the fragmented sample overnight at -20℃, and then place it in a freeze dryer and freeze-dry it according to the following program: -25℃, 240 min, -20℃, 120 min, -15℃, 90 min, -10℃, 120 min, -5℃, 120 min, -2℃, 120 min, 0℃, 120 min, 5℃, 60 min, 8℃, 60 min, 10℃, 120 min, 20℃, 120 min, 25℃, 260 min.

[0035] (4) Silage drying: The crushed sample is put into a silage bag, sealed and degassed, and then silaged for 30 days. After silage is completed, it is placed in an oven at 70 ℃ and dried to constant weight to obtain the silage dried sample.

[0036] (5) Silage freeze-drying: The crushed sample is put into a silage bag, sealed and degassed, and then silaged for 30 days. After silage is completed, it is freeze-dried according to (3) to obtain the silage freeze-dried sample.

[0037] Determine the content of total phenols and total flavonoids, and remove DPPH and ABTS. + Free radical reduction capacity and FRAP iron ion reducing capacity (detection methods are described at the end of the text). Detection results are as follows: Figure 1 and Figure 2 As shown, the total phenol and total flavonoid content of freeze-dried and ensilized lotus seedpods is higher than that of oven-dried and sun-dried lotus seedpods. The antioxidant capacity of freeze-dried and ensilized lotus seedpods is superior to that of sun-dried, oven-dried, and oven-dried ensilized lotus seedpods. There is no significant difference in antioxidant capacity between freeze-dried and ensilized lotus seedpods after 30 days, while sun-dried lotus seedpods exhibit the worst antioxidant capacity. The freeze-dried samples were obtained by directly freeze-drying fresh lotus seedpods, a process that takes only about 2 days. The ensilized freeze-dried samples were freeze-dried after 30 days of ensiling. This time difference indicates that ensiling can extend the preservation of active substances in lotus seedpods. Since lotus seedpods are harvested in batches, ensiling can be used for temporary storage. The obtained lotus seedpods can be ensilized in batches and then dried at the same time. Simultaneously, ensiling can effectively increase the content of active substances in lotus seedpods. In other words, using ensiling technology as a temporary storage method can effectively maintain the stability and integrity of bioactive substances in lotus seedpods.

[0038] Example 2: Improving the antioxidant capacity of lotus seedpod shells Lactobacillus plantarum CCZZ1 has been disclosed in Chinese patent CN 102851233B, with accession number CGMCCNO.6078. MRS culture was performed using a shake flask culture method at 37℃, followed by centrifugation at 10000 rpm and resuspending in deionized water to obtain a concentration of 1.0 × 10⁻⁶. 8 CFU CCZZ1 bacterial suspension.

[0039] Lactic acid bacteria ( Lactobacillus parafarraginis ZH1 has been disclosed in Chinese patent CN 102851232 B, accession number CGMCC NO.6079. MRS culture was performed using shake flask culture at 37℃, followed by centrifugation at 10000 rpm and resuspending in deionized water to obtain a concentration of 1.0 × 10⁻⁶. 8 CFU ZH1 bacterial suspension.

[0040] A silage slurry addition experiment was conducted using lotus seedpod shells (the nutritional characteristics before silage are shown in Table 1) as the material. The material was chopped to 2–3 cm, mixed thoroughly, and the resulting fragments were set up as follows: control group (CK, with an equal volume of sterile water added to the bacterial solution), and group with the addition of homologous lactic acid bacteria CCZZ1 (CZ1, 1.0 × 10⁻⁶). 8 CFU / kg lotus seedpod shell), heterofermentative lactic acid bacteria ZH1 (ZH1, 1.0 × 10⁻⁶ CFU / kg lotus seedpod shell), 8The silage was prepared by adding cfu / kg lotus seedpod shells, glucose (G, added at 2% of the weight of lotus seedpod shells), and cellulase (C, added at 0.02% of the weight of lotus seedpod shells). The silage was packed into 30 cm × 20 cm polyethylene bags, with three replicates per treatment. Each bag contained approximately 200 g of silage. The bags were degassed using a vacuum sealer (SINBO Vacuum Sealer, Hong Tai Home Electrical Appliance Co., Ltd.), sealed, and stored in the dark for 15, 30, and 60 days. After opening, the pH, total phenols, total flavonoids, and antioxidant capacity (DPPH and ABTS scavenging) were analyzed. + Free radical reduction capacity and FRAP iron ion reducing capacity were compared and analyzed. Staphylococcus aureus inhibition tests were conducted on sun-dried lotus seedpod shells, CK and G freeze-dried samples after 30 days of silage.

[0041] The test results are shown in Tables 1 and 2. Figure 3 , Figure 4 and Figure 5 As shown: Lotus seedpod shells have high fiber content and buffering energy, low water-soluble carbohydrate content, and a high number of undesirable microorganisms (Table 1). Natural ensiling and fermentation with the addition of lactic acid bacteria CCZZ1 and ZH1 alone have poor fermentation effects (Table 2). The pH of lotus seedpod shells with 2% glucose added after ensiling for 15, 30, and 60 days is significantly lower than that of other treatments. P <0.05, the additive 2% glucose has a highly significant effect on the pH of lotus seedpod shells ( P <0.01 (Table 2); with the extension of silage time, the total phenolic and total flavonoid content and antioxidant capacity of lotus seedpod shells decreased. The total phenolic and total flavonoid content and antioxidant capacity of silage with 2% glucose added at 15 days and 30 days were significantly higher than those of other treatments. P <0.05%, with the best effect observed when 2% glucose was added after 30 days; the total phenol and total flavonoid content and antioxidant capacity of freeze-dried products were higher than those of dried products ( Figure 3 , Figure 4 Lotus seedpod shell polyphenol extract can inhibit the growth of Staphylococcus aureus, and the inhibitory effect is best when 2% glucose is added. Figure 5 ).

[0042] Table 1. Characteristics and microbial count of lotus seedpod shell raw materials (±SD, n=3)

[0043] Note: DM, silage dry matter; FM, silage fresh matter; CFU, colony forming unit.

[0044] Table 2. Effects of different additives on pH of lotus seed silage.

[0045] Note: Different lowercase letters in the same column indicate significant differences. P <0.05); different capital letters in the same row indicate significant differences ( P <0.05). CK: Control; CZ1: Added with homofermentative lactic acid bacteria Lactobacillus plantarum CCZZ1; ZH1: Added with heterofermentative lactic acid bacteria Lactobacillus fragrans ZH1; G: Added with 2% glucose; C: Added with 0.02% cellulase; D: Silage days; T: Additive; D*T: Interaction; **: Significance P <0.01; *: significance P <0.05; NS: No significant difference.

[0046] Example 3: Adding lotus seedpod shells improves the fermentation quality of purple elephant grass silage. A silage addition experiment was conducted using purple elephant grass and lotus seedpods as materials. Purple elephant grass was collected at the experimental base behind the fifth teaching building of South China Agricultural University. The purple elephant grass was chopped to 2-3 cm and then mixed evenly. Fresh lotus seedpods were dried according to (2) in Example 1, and the dried sample was crushed (passed through a 40-mesh sieve) and used as a silage additive. A control group (purple elephant grass only) and 0.5%, 1%, 2%, 4%, and 8% lotus seedpod powder were added (the amount of lotus seedpod powder was based on purple elephant grass). The silage was packed into 30 cm × 20 cm polyethylene silage bags, with 3 replicates for each treatment. Each bag contained approximately 200 g of silage. The bags were degassed using a vacuum sealer (SINBO VacuumSealer, Hong Tai Home Electrical Appliance Co. Ltd.), sealed, and stored in the dark for 30 days. After opening, the pH and organic acid content were analyzed and compared.

[0047] Test results as follows Figure 6 As shown: the pH of purple elephant grass silage with the addition of 4% lotus seedpod powder decreased to 3.96, which was significantly lower than the control and other treatments with different proportions of lotus seedpod powder added. P <0.05), and the lactic acid content of the treatment with 4% lotus seedpod powder was higher, reaching 2.20% DM, which was significantly higher than other treatments ( P <0.05). Therefore, adding 4% lotus seedpod powder can effectively improve the silage fermentation quality of purple elephant grass.

[0048] Total phenols, total flavonoids, antioxidant capacity (scavenging DPPH, ABTS) + Free radical capacity, FRAP iron ion reducing capacity, nutrient composition and silage fermentation indicators, and antibacterial test methods: 1) Total phenols, total flavonoids, and antioxidant capacity (scavenging DPPH and ABTS) +Analysis of free radical capacity and FRAP iron ion reducing capacity: Weigh 0.2 g of dried lotus seed shell powder, put it into a 15 mL centrifuge tube, add 10 mL of methanol, and extract on a shaker for 24 h (200 rpm, protected from light). Centrifuge the extract at 3000 rpm for 10 min, and take at least 7 mL of the supernatant for subsequent index determination.

[0049] 1.1) Total phenols (mg GAE / g DM): Prepare a 1 mg / mL gallic acid solution (dissolved in pure water) as a standard curve, dilute to a concentration of 0–80 μg / mL, add 0.2 mL of extract, 2.6 mL of pure water, and 0.2 mL of Folin reagent, shake well, and incubate at room temperature in the dark for 6 min. Add 2 mL of 7% w / w Na2CO3 solution, shake well, and incubate at room temperature in the dark for 90 min. Measure the absorbance at 750 nm.

[0050] 1.2) Total flavonoids (mg RE / g DM): Prepare a 1 mg / mL rutin solution (dissolved in methanol) as a standard curve, and dilute to 0–1000 μg / mL. Take 0.5 mL each of the rutin solution of different concentrations of the standard curve, the methanol blank control group, and the extracts before and after dilution (the extracts were diluted with methanol). Add 0.15 mL of 5% w / w NaNO2 solution. After 6 min, add 0.15 mL of 10% w / w AlCl3 solution. After 6 min, add 2 mL of 4% w / w NaOH solution (all need to be shaken well and protected from light). Add 2.2 mL of water to form a system with a total volume of 5 mL. Let stand in the dark for 10 min, and measure the absorbance at 510 nm.

[0051] 1.3) DPPH radical scavenging capacity (mg TE / g DM): Prepare a 1 mg / mL trolox solution (dissolved in methanol) as a standard curve, and dilute it to a concentration of 0–80 μg / mL. Take 0.5 mL of trolox solution of different concentrations from the standard curve, the methanol blank control group, and the extracts before and after dilution, respectively, and then add 4 mL of DPPH (0.1 mM / L, dissolved in methanol) solution. Shake vigorously to mix well, let stand in the dark for more than 30 min, and measure the absorbance at 517 nm.

[0052] 1.4) ABTS + Free radical scavenging capacity (mg TE / g DM) was assessed by preparing a 1 mg / mL trolox solution (dissolved in methanol) as a standard curve, diluted to concentrations ranging from 0 to 250 μg / mL. An ABTS stock solution was prepared using pure water, consisting of a 7 mM ABTS solution and a 2.45 mM potassium persulfate solution in a 1:1 volume ratio. After shaking well and incubating in the dark for approximately 16 hours, the solution was diluted 20 times before use, yielding the OD value. 734(Approximately 0.7). Take trolox solutions of different concentrations of the standard curve, add 0.2 mL each to the methanol blank control group and the extracts before and after dilution, then add 4 mL of diluted ABTS solution, shake to mix evenly, and let stand in the dark for 6 min. Measure the absorbance at 734 nm.

[0053] 1.5) FRAP iron reducing power (mg TE / g DM): Prepare a 1 mg / mL trolox solution (dissolved in methanol) as a standard curve, and dilute to a concentration of 0–80 μg / mL. Mix 10 mM nitrotriazole TPTZ solution (adjusted to volume with 40 mM hydrochloric acid), 20 mM FeCl3·6H2O solution, and 300 mM acetate buffer (20.4 g sodium acetate + 80 mL glacial acetic acid, adjusted to 1 L) at a volume ratio of 1:1:10, and heat in a water bath at 37°C for 30 min. Take 0.5 mL each of the trolox solution at different concentrations of the standard curve, the methanol blank control group, and the extracts before and after dilution, add 4 mL of FRAP working solution, mix thoroughly, and incubate at 37°C in the dark for 30 min. Measure the absorbance at 593 nm.

[0054] 2) Determination of forage nutrient composition and microbiological analysis: Dry matter (DM) content was determined using the 70℃ drying method described in Zhang Liying's Feed Analysis; crude protein content was determined using the Kjeldahl nitrogen determination method (KN680 nitrogen analyzer, ALVA Instruments Co., Ltd.); neutral detergent fiber and acid detergent fiber content were determined using the filter bag method; crude fat content was determined using the ether extraction method (SLF-06, Hangzhou Top Instruments Co., Ltd.); and crude ash content was determined using the ignition method. Water-soluble carbohydrate (WSC) content was determined using the anthrone-sulfuric acid method; buffer energy was determined using the hydrochloric acid and sodium hydroxide titration method; and the numbers of bacteria, lactic acid bacteria, coliform bacteria, yeast, and mold were counted using nutrient agar, MRS (de-Man Rogosa Sharpe) agar, violet red bile agar, and potato dextrose agar (Guangdong Huankai Microbiology Co., Ltd.). Lactic acid bacteria were cultured in an anaerobic incubator at 37°C for 1–2 days; coliform bacteria, bacteria, yeasts, and molds were cultured in a biochemical incubator at 30°C for 2–4 days.

[0055] 3) Fermentation quality analysis: After opening the silage bags, 20 g of the mixed silage was placed in a resealable bag, 80 mL of distilled water was added, and the mixture was soaked in a 4℃ refrigerator for 18 h. After filtration, the pH of the extract was measured using a pH meter (Mettler Toledo FE28 pH meter). The organic acid content was determined using a Shimadzu LC-20AT high-performance liquid chromatograph: Chromatographic conditions: column (Eleven Organic Acids on Transgenomic COREGel 87H3), detector: RID-10A, mobile phase: 0.1 mmol / L phosphoric acid solution, flow rate: 1 mL / min, column temperature: 40 ℃, detection wavelength: 210 nm, injection volume: 20 µL.

[0056] 4) Antibacterial test analysis: Sun-dried lotus seedpod shells, CK and G freeze-dried samples after 30 days of silage were pulverized, passed through a 40-mesh sieve, and sealed for later use. 1.0 g of lotus seedpod shell powder was weighed and pretreated for polyphenol extraction at 60 ℃, ultrasonic power 80 W, extraction time 43 min, extraction solution was a 40% (v / v) ethanol-water solution, and a material-to-liquid ratio of 1:40 g / mL. After centrifugation, the supernatant was filtered, and the residue was repeated. The filtrate was concentrated by rotary evaporation and then freeze-dried to obtain crude polyphenol extract powder from lotus seedpod shells. A 100 mg / mL polyphenol extract was prepared using dimethyl sulfoxide as the solvent; ampicillin sodium was used as the antibiotic, prepared at a concentration of 100 μg / mL. Staphylococcus aureus subsp. aureus was cultured in advance. Staphylococcus aureussubsp. aureus (GDMCC 1.1220, purchased from Guangdong Provincial Microbial Culture Collection Center). Inoculate the bacteria from the frozen tubes into 6 mL LB liquid medium and incubate for 24 h, then transfer 200 μL to another 6 mL LB liquid medium and incubate for another 24 h. Pour 20 mL LB solid medium into a petri dish, setting up three replicates. Take 100 μL of the cultured bacterial solution and spread it evenly onto the medium, then punch five wells using a 6 mm diameter punch. Add the corresponding three different treatment polyphenol extracts to the top three wells, and add antibiotics as a positive control and dimethyl sulfoxide as a negative control to the bottom two wells, respectively. Add 20 μL of test sample to each well.

[0057] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A method for storing lotus seedpod shells to enhance their active ingredients, characterized in that... Includes the following steps: (1) Chop fresh lotus seedpods and mix them with glucose to obtain a mixture; (2) The mixture is ensilized and then dried to obtain lotus seedpod shell granules with increased active ingredients.

2. The method for storing lotus seedpod shells to enhance their active ingredients according to claim 1, characterized in that: The degree of chopping mentioned in step (1) is to chop to a length of 2-3 cm; The drying method described in step (2) is either oven drying or freeze drying.

3. The method for storing lotus seedpod shells to enhance their active ingredients according to claim 1, characterized in that: The amount of glucose used in step (1) is calculated as 1 to 3% of the mass of the lotus seedpod shell.

4. The method for storing lotus seedpod shells to enhance their active ingredients according to claim 1, characterized in that: The silage time mentioned in step (2) is 15 to 60 days.

5. The application of the storage method according to any one of claims 1 to 4 in the processing of lotus seedpod shells.

6. A product, characterized in that: Lotus seedpod shell granules with enhanced active ingredients are obtained by the storage method described in any one of claims 1 to 4.

7. The use of the product according to claim 6 in the preparation of natural antioxidants and antibacterial agents.

8. Application of lotus seedpod shells as silage additives.

9. The application according to claim 8, characterized in that... The process includes the following steps: adding dried lotus seedpod powder to the silage raw materials, mixing them evenly, and then ensiling them to obtain silage.

10. The application according to claim 9, characterized in that: The lotus seedpod shell powder is the lotus seedpod shell granules with enhanced active ingredients as described in claim 6; The silage feed raw material is purple elephant grass.

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