Anti-aging and whitening essential oil lotion and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to anti-aging and whitening essential oil dew and a preparation method thereof. The anti-aging and whitening essential oil lotion comprises a water phase, an oil phase and a wrapping system, the wrapping system comprises lipidosome and a cyclodextrin inclusion compound; a first active component is encapsulated in the liposome, and the first active component comprises at least one of rosa damascena flower oil, meadowfoam seed oil, raspberry seed oil, tocopherol and ascorbyl tetraisopalmitate; the cyclodextrin inclusion compound comprises a second active component, and the second active component comprises at least one of 4-butylresorcinol, glabridin and acetylchitosamine. According to the invention, the technical bottlenecks of the multi-active-ingredient functional cosmetics in the aspects of stability, transdermal property and efficacy synergy are systematically overcome in principle through an innovative double-synergistic wrapping system and a corresponding preparation method; the invention provides an essential oil lotion product with excellent comprehensive performance and anti-aging and whitening effects and an efficient and reliable preparation method thereof.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to an anti-aging whitening essential oil lotion and a preparation method thereof. BACKGROUND

[0002] With the maturation of consumers' skin care concepts, cosmetics with both anti-aging and whitening effects have become the mainstream demand in the market. To achieve this comprehensive effect, the product formula usually needs to compound multiple active ingredients, including natural plant extracts, vitamin derivatives, polypeptides, and specific whitening compounds. These ingredients work together on multiple signaling pathways in the skin through different mechanisms of action, such as antioxidant, inhibition of tyrosinase, promotion of collagen synthesis, and anti-glycation, in order to achieve synergistic effect.

[0003] However, the efficient integration of a variety of active ingredients with different physicochemical properties into a single formula system still faces severe technical challenges, directly affecting the final performance of multi-effect products. The main difficulties are as follows: first, the stability problem is prominent. Many efficient whitening and anti-aging ingredients are chemically active, and in complex aqueous or oil phase environments, especially under the influence of light or heat, they are prone to degradation or oxidation and lose activity. At the same time, the long-term storage of active ingredients with different properties in a simple mixed system can easily cause phase separation, precipitation, or cream demulsification, affecting product appearance and user experience. Second, the transdermal absorption efficiency is low. The stratum corneum as a protective barrier makes it difficult for many macromolecules or polar / non-polar mismatched active ingredients to effectively penetrate the target layer. Third, the synergistic release of multiple components is difficult to accurately control. An ideal anti-aging and whitening formula expects ingredients with different mechanisms of action to act on different layers of the skin at the right time and at the right rate. However, in traditional emulsion or solution systems, the release behavior of each component tends to be consistent, making it difficult to achieve differential delivery for components with different solubility characteristics and release needs. SUMMARY

[0004] The purpose of the present application is to solve the problems in the prior art, provide an anti-aging whitening essential oil lotion and a preparation method thereof, which systematically overcome the technical bottlenecks of multi-active ingredient efficacy cosmetics in stability, transdermal properties, and efficacy synergy from the principle by innovative dual synergistic packaging system and corresponding preparation method, and provide an essential oil lotion product with excellent comprehensive performance, anti-aging and whitening effects, and an efficient and reliable preparation method thereof.

[0005] To achieve the above purpose, the present application provides an anti-aging whitening essential oil lotion, comprising an aqueous phase, an oil phase, and a packaging system; the packaging system comprises liposomes and cyclodextrin inclusion compounds. The liposome encapsulates a first active ingredient, and the first active ingredient comprises at least one of damask rose flower oil, white pool seed oil, raspberry seed oil, tocopherol, ascorbic acid tetraisopalmitate; the cyclodextrin inclusion complex includes at least one of 4-butyl resorcinol, glabridin, acetylated chitosamine.

[0006] Preferably, the water phase comprises water, glycerol, butylene glycol and p-hydroxyacetophenone; the oil phase comprises squalane, hexyldecanol and caprylic / capric triglyceride.

[0007] Preferably, the water phase further comprises at least one of damask rose flower water, propolis extract, houttuynia cordata extract, spilanthes acmella extract, chamomile extract, radix scutellariae extract, peony root bark extract.

[0008] Preferably, the wall material of the liposome comprises at least one of hydrogenated lecithin and lecithin; the cyclodextrin is selected from hydroxypropyl-β-cyclodextrin.

[0009] Preferably, in the encapsulation system, the weight ratio of the liposome and the cyclodextrin inclusion complex is 1: (0.5-2).

[0010] Preferably, the following components are included in percentage by weight: damask rose flower water 5-15%, glycerol 3-8%, butylene glycol 2-6%, p-hydroxyacetophenone 0.1-0.5%, propolis extract 0.1-2%, houttuynia cordata extract 0.01-0.1%, squalane 1-5%, hexyldecanol 0.5-2%, caprylic / capric triglyceride 1-5%, spilanthes acmella extract 0.1-1%, chamomile extract 0.1-1%, radix scutellariae extract 0.1-1%, peony root bark extract 0.1-1%, encapsulation system 1-10%, and the balance is water.

[0011] The application also provides a method for preparing the anti-aging whitening essential oil lotion, comprising the following steps: S1. preparing a liposome dispersion and a cyclodextrin inclusion complex, respectively; mixing and emulsifying the water phase components and the oil phase components to obtain a primary emulsion; S2. mixing the liposome dispersion, the cyclodextrin inclusion complex and the primary emulsion to obtain the anti-aging whitening essential oil lotion.

[0012] Preferably, in S1, the process for preparing the liposome dispersion comprises: dissolving the wall material of the liposome in an organic solvent, and forming a lipid membrane after removing the solvent; mixing the first active ingredient with a buffer solution, and hydrating and ultrasonic treating the lipid membrane to obtain the liposome dispersion. The process for preparing the cyclodextrin inclusion compound comprises: dissolving cyclodextrin in water, mixing with the second active ingredient, then sequentially performing stirring reaction and ball milling treatment, and obtaining the cyclodextrin inclusion compound after filtration and drying.

[0013] Preferably, in S1, the temperature of the mixing is 50-60℃; the stirring speed of the emulsification is 2500-3500r / min, and the time is 3-7min.

[0014] Preferably, in S2, the temperature of the mixing is 35-45℃, the stirring speed is 300-700r / min, and the time is 35-60min. After the mixing, the pH value is adjusted to 5.0-6.5 by using a citric acid solution.

[0015] The present application has the following advantages: (1) The present application adopts different packaging strategies according to the physical and chemical properties of different active ingredients. The first active ingredients such as spike-rose oil and ascorbic acid tetraisopalmitate are encapsulated by using the phospholipid bilayer structure of liposomes to provide a stable lipophilic microenvironment; the second active ingredients such as 4-butylresorcinol and glabridin are included by using the cavity structure of cyclodextrin, which is "hydrophobic inside and hydrophilic outside", to improve their dispersibility and chemical stability in aqueous systems. The double packaging system physically isolates the components which are easily interfered with each other or sensitive to the environment, effectively avoiding the degradation, oxidation, precipitation or phase separation of the active ingredients during storage, and essentially enhancing the physical and chemical stability of the whole formula.

[0016] (2) The liposome and cyclodextrin inclusion compound are not only protective carriers, but also functional transdermal delivery systems. The liposome can efficiently promote the transdermal penetration of the loaded active ingredients by fusing with the stratum corneum due to its similar phospholipid bilayer structure to biological membranes; the cyclodextrin inclusion compound significantly enhances the transdermal absorption efficiency of the active ingredients by increasing their apparent solubility and diffusion coefficient. The synergistic effect of the two achieves the simultaneous and efficient delivery of active ingredients with different solubility characteristics. At the same time, the system allows independent design of different carriers, thereby potentially regulating the release kinetics of different active ingredients, making it possible to achieve the synergistic effect of rapid and long-acting, shallow and deep-acting, overcoming the limitation that all components in traditional formulations have similar release behaviors.

[0017] (3) Through the synergistic improvement of stability and transdermal efficiency, the various anti-aging and whitening active ingredients in the formula of the present application can maximize the preservation of their original chemical structure and biological activity during the product shelf life. When the product is used, these effectively protected and transdermally optimized active ingredients can more efficiently reach the skin target, fully exerting their biological functions such as inhibiting tyrosinase, scavenging free radicals, and antioxidant, thereby achieving persistent and significant anti-aging and whitening dual effects.

[0018] The preparation method of the present application separates the preparation of functional inclusion bodies (liposomes, cyclodextrin inclusions) from the preparation process of the base emulsion, and mixes the pre-prepared inclusion bodies with the initial emulsion after cooling in a mild post-addition manner, effectively avoiding the loss of heat-sensitive or easily-oxidized active ingredients in the traditional high-temperature emulsification process. The method is simple, mild and reproducible, and is easy to realize industrial scale-up production. The obtained essential oil lotion product has a refreshing and delicate texture, a superior skin feel, and a good application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 is a schematic diagram of the tyrosinase inhibitory activity of different samples in experimental example 3 of the present application; Figure 2 is a schematic diagram of the DPPH free radical scavenging effect of different samples in experimental example 4 of the present application. DETAILED DESCRIPTION

[0020] The present application provides an anti-aging whitening essential oil lotion, which comprises an aqueous phase, an oil phase and a packaging system; the packaging system comprises liposomes and cyclodextrin inclusions. The liposomes encapsulate a first active ingredient, and the first active ingredient comprises at least one of tuberose flower oil, white pool seed oil, raspberry seed oil, tocopherol and ascorbic acid tetraisopalmitate; the cyclodextrin inclusions include at least one of 4-butyl resorcinol, glabridin and acetylated chitosamine.

[0021] In the present application, the aqueous phase comprises water, glycerol, butylene glycol and p-hydroxyacetophenone; the oil phase comprises squalane, hexyldecanol and caprylic / capric triglyceride.

[0022] In the present application, the aqueous phase further comprises at least one of tuberose flower water, propolis extract, houttuynia cordata extract, spilanthes acmella extract, chamomile extract, radix scutellariae extract and peony root bark extract.

[0023] The above-mentioned tuberose flower water, propolis extract, houttuynia cordata extract, spilanthes acmella extract, chamomile extract, radix scutellariae extract and peony root bark extract are all water-soluble plant extracts commonly used in the art, which can be obtained commercially or prepared by conventional water extraction or alcohol extraction.

[0024] In the present application, the wall material of the liposomes comprises at least one of hydrogenated lecithin and lecithin; the cyclodextrin is selected from hydroxypropyl-β-cyclodextrin.

[0025] In the present application, the average particle size of the liposomes is 50-200 nm.

[0026] In the present application, the weight ratio of the liposome and the cyclodextrin inclusion compound in the encapsulation system is 1: (0.5-2). The weight ratio here refers to the ratio of the weight of the solid matter contained in the liposome dispersion and the weight of the cyclodextrin inclusion compound.

[0027] In the present application, by weight percentage (the total is 100%), the following components are included: Rosa damascena flower water 5-15%, glycerol 3-8%, butanediol 2-6%, p-hydroxyacetophenone 0.1-0.5%, propolis extract 0.1-2%, Houttuynia cordata extract 0.01-0.1%, squalane 1-5%, hexyldecanol 0.5-2%, caprylic / capric triglyceride 1-5%, purslane extract 0.1-1%, golden chamomile extract 0.1-1%, radix scutellariae extract 0.1-1%, peony root bark extract 0.1-1%, encapsulation system 1-10%, and the balance is water.

[0028] The present application also provides a method for preparing the anti-aging whitening essential oil lotion, comprising the following steps: S1. Preparing a liposome dispersion and a cyclodextrin inclusion compound, respectively; Mixing and emulsifying the water phase components and the oil phase components to obtain a primary emulsion; S2. Mixing the liposome dispersion, the cyclodextrin inclusion compound, and the primary emulsion to obtain the anti-aging whitening essential oil lotion.

[0029] In the present application, in S1, the process for preparing the liposome dispersion comprises: dissolving the wall material of the liposome in an organic solvent, forming a lipid membrane after removing the solvent; heating and dissolving the first active ingredient, and mixing with a phosphate buffer solution to obtain a mixed solution; hydrating and ultrasonic treating the lipid membrane with the mixed solution to obtain the liposome dispersion.

[0030] In the present application, in S1, the process for preparing the cyclodextrin inclusion compound comprises: dissolving the cyclodextrin in water, adding the second active ingredient, and sequentially performing stirring reaction and ball milling treatment to obtain a slurry; filtering and drying the slurry to obtain the cyclodextrin inclusion compound.

[0031] In the present application, in S1, the temperature of the mixing is 50-60℃; the rotation speed of the emulsification is 2500-3500 r / min, and the time is 3-7 min.

[0032] In the present application, in S2, the temperature of the mixing is 35-45℃, the rotation speed is 300-700 r / min, and the time is 35-60 min.

[0033] In the present application, in S2, after the mixing is completed, it further comprises: adjusting the pH value to 5.0-6.5 with a citric acid solution.

[0034] The following examples are provided to better enable those skilled in the art to which the application pertains to further understand the application and are not intended to limit the scope of the application, which is defined by the appended claims. Any product that is the same as or similar to the present application derived from the disclosure of the present application or from the combination of the present application with other prior art features falls within the scope of the present application.

[0035] Unless otherwise indicated, conventional methods of preparing and isolating the compounds described herein are employed, as described in standard laboratory manuals such as Fieser and Fieser's Reagents for Organic Synthesis, Volumes 1-15, Wiley, 1991; Rodd's Chemistry of Carbon Compounds, Volumes 1-5 and 6, Elsevier, 1989; Organic Reactions, Wiley, 1991; March's Advanced Organic Chemistry, Wiley, 5th Edition, 2001; and Larock's Comprehensive Organic Transformations, Viley, 1999.

[0036] Example 1 The present embodiment provides a preparation method of an anti-aging whitening essential oil lotion, comprising the following steps: Formulation (in percentage by weight): Rosa damascena flower water 10%, glycerin 5%, butylene glycol 4%, p-hydroxyacetophenone 0.3%, propolis extract 0.5%, Houttuynia cordata extract 0.05%, squalane 3%, hexyldecanol 1%, caprylic / capric triglyceride 3%, purslane extract 0.5%, golden yellow chamomile extract 0.3%, radix scutellariae extract 0.3%, peony root bark extract 0.3%, encapsulation system 5%, and the balance being water.

[0037] In the encapsulation system, the weight ratio of liposomes and cyclodextrin inclusion compound is 1:1. The liposomes encapsulate the first active ingredient: white pool flower seed oil and ascorbyl tetraisopalmitate. The cyclodextrin inclusion compound includes the second active ingredient: 4-butylresorcinol and glabridin.

[0038] Preparation: Hydrogenated lecithin 0.8 g and lecithin 0.4 g were weighed and dissolved in 10 mL of ethanol to form a lipid film by rotary evaporation. 0.3 g of white pool flower seed oil and 0.2 g of ascorbyl tetraisopalmitate were mixed and heated to 50°C to dissolve, and then added to 20 mL of preheated phosphate buffer (pH 6.5) at 60°C. The mixture was dispersed by slight vortex to obtain a mixed solution. The mixed solution was poured into a flask containing the lipid film, and hydrated at 60°C for 30 min, followed by ultrasonic treatment at 350W power in an ice bath for 8 min. The solution was filtered through a 0.45 μm filter to obtain a liposome dispersion (the average particle size of the liposomes was 118 nm).

[0039] Hydroxypropyl-β-cyclodextrin 2.0 g was dissolved in 20 mL of water, and 0.2 g of 4-butylresorcinol and 0.2 g of glabridin were added. After stirring and reacting in a 55°C water bath for 2 h, ball milling was performed for 60 min. The obtained slurry was filtered and freeze-dried to obtain a cyclodextrin inclusion compound powder.

[0040] Add the formula amount of water into the emulsifying kettle and heat to 55℃. Add the formula amount of Rosa rugosa thunb. flower water, glycerin, butylene glycol, p-hydroxyacetophenone, propolis extract, Houttuynia cordata extract, Portulaca oleracea extract, Anthemis nobilis extract, Scutellaria baicalensis root extract, Paeonia suffruticosa root bark extract, and squalane, hexyldecanol, caprylic / capric triglyceride in sequence. Homogenize for 5 min at 3000 r / min, then slowly cool to 40℃, to obtain a primary emulsion.

[0041] Add the liposome dispersion and cyclodextrin inclusion powder into the primary emulsion and stir at 40℃ and 500 r / min for 50 min to fully mix and homogenize. Adjust the pH of the system to 5.8 with a 10% citric acid solution. Finally, filter through a 200-mesh sieve, fill, and obtain the anti-aging whitening essential oil lotion.

[0042] Example 2 The present example provides a preparation method of an anti-aging whitening essential oil lotion, comprising the following steps: The difference between the formula and Example 1 is only that: The weight percentage of the coating system is adjusted to 3% (replaced with an equal amount of water); the first active ingredient encapsulated by the liposome is adjusted to Rosa rugosa thunb. flower oil and tocopherol; and the second active ingredient included by the cyclodextrin inclusion is adjusted to 4-butylresorcinol and acetylated chitosamine.

[0043] The difference between the preparation and Example 1 is only that: (1) In the preparation of the liposome dispersion, “0.3 g of white pool flower seed oil and 0.2 g of ascorbic acid tetraisopalmitate” are replaced with “0.3 g of Rosa rugosa thunb. flower oil and 0.2 g of tocopherol”; in the preparation of the cyclodextrin inclusion powder, “0.2 g of 4-butylresorcinol and 0.2 g of glabridin” are replaced with “0.2 g of 4-butylresorcinol and 0.2 g of acetylated chitosamine”; (2) In the preparation of the primary emulsion, the homogenization speed is adjusted to 2800 r / min and the time is adjusted to 6 min; (3) In the preparation of the anti-aging whitening essential oil lotion, the stirring conditions are adjusted to stirring at 38℃ and 500 r / min for 52 min.

[0044] Example 3 The present example provides a preparation method of an anti-aging whitening essential oil lotion, comprising the following steps: The difference between the formula and Example 1 is only that: The weight percentage of the coating system is adjusted to 8% (replaced with an equal amount of water); the weight ratio of the liposome and the cyclodextrin inclusion is 1:0.8, and the first active ingredient encapsulated by the liposome is adjusted to raspberry seed oil and ascorbic acid tetraisopalmitate.

[0045] The preparation is different from example 1 only in that: (1) When preparing the liposome dispersion, replace "0.3g white pool seed oil and 0.2g ascorbic acid tetraisopalmitate" with "0.3g raspberry seed oil and 0.2g ascorbic acid tetraisopalmitate"; (2) When preparing the primary emulsion, adjust the rotation speed of the homogenizing emulsification to 3200r / min and the time to 4min; (3) When preparing the anti-aging whitening essential oil lotion, adjust the stirring conditions to stirring at 42℃ and a rotation speed of 500r / min for 47min.

[0046] Example 4 The present example provides a preparation method of an anti-aging whitening essential oil lotion, comprising the following steps: The formula is different from example 1 only in that: The weight ratio of liposomes and cyclodextrin inclusion is 1:1.5, and the first active ingredient encapsulated by liposomes is adjusted to white pool seed oil and damask rose oil.

[0047] The preparation is different from example 1 only in that: When preparing the liposome dispersion, replace "0.3g white pool seed oil and 0.2g ascorbic acid tetraisopalmitate" with "0.3g white pool seed oil and 0.2g damask rose oil".

[0048] Comparative example 1 The present comparative example provides a preparation method of an anti-aging whitening essential oil lotion without wrapping, comprising the following steps: Formula (in percentage by weight): Damask rose water 10%, glycerol 5%, butanediol 4%, p-hydroxyacetophenone 0.3%, propolis extract 0.5%, fishwort extract 0.05%, squalane 7.1%, hexyldecanol 1%, caprylic / capric acid triglyceride 3%, spilanthes acmella willd extract 0.5%, chamomile extract 0.3%, radix scutellariae extract 0.3%, peony root extract 0.3%, white pool seed oil 0.3%, ascorbic acid tetraisopalmitate 0.2%, 4-butylresorcinol 0.2%, glabridin 0.2%, and the balance is water.

[0049] Preparation: Mix white pool seed oil, ascorbic acid tetraisopalmitate, and the first part of squalane (the weight ratio of the first part of squalane to the total squalane is 4.1:7.1), heat to 50℃ and stir to dissolve to obtain the first active substance; 4-butylresorcinol and glabridin are respectively assisted to dissolve with the first part of butanediol (the weight ratio of the first part of butanediol to the total butanediol is 2:4), and slightly heated and stirred until evenly dispersed to obtain the second active substance.

[0050] The formula amount of water was added into an emulsifying pot and heated to 55°C. The formula amount of Rosa rugosa petals water, glycerin, the remaining butylene glycol, p-hydroxyacetophenone, propolis extract, Houttuynia cordata extract, Spilanthes acmella extract, Anthemis nobilis extract, Scutellaria baicalensis root extract, Paeonia suffruticosa root bark extract and the second active were sequentially added to obtain a first mixture. Squalane, hexyldecanol, caprylic / capric triglyceride and the first active were mixed to obtain a second mixture. The second mixture was slowly added into the first mixture, homogenized at a rotation speed of 3000 r / min for 5 min, and then slowly cooled to 40°C. The pH value of the system was adjusted to 5.8 with a 10% citric acid solution. Finally, filtration was performed through a 200-mesh screen, filling was performed, and an anti-aging whitening essential oil lotion was obtained.

[0051] Comparative Example 2 This comparative example provides a preparation method of an anti-aging whitening essential oil lotion, only single encapsulation of liposomes is performed, which specifically comprises the following steps: Formula (in percentage by weight): Rosa rugosa petals water 10%, glycerin 5%, butylene glycol 4%, p-hydroxyacetophenone 0.3%, propolis extract 0.5%, Houttuynia cordata extract 0.05%, squalane 3%, hexyldecanol 1%, caprylic / capric triglyceride 3%, Spilanthes acmella extract 0.5%, Anthemis nobilis extract 0.3%, Scutellaria baicalensis root extract 0.3%, Paeonia suffruticosa root bark extract 0.3%, encapsulation system 5%, and the balance being water.

[0052] The encapsulation system was liposomes, and the liposomes encapsulated white pool flower seed oil, ascorbyl tetraisopalmitate, 4-butylresorcinol and glabridin.

[0053] Preparation: Hydrogenated lecithin 1.0 g and lecithin 0.5 g were weighed, dissolved in 12 mL of ethanol, and rotary evaporation was performed to form a lipid film. 0.3 g of white pool flower seed oil, 0.2 g of ascorbyl tetraisopalmitate, 0.2 g of 4-butylresorcinol and 0.2 g of glabridin were added together into 25 mL of preheated phosphate buffer (pH 6.5) at 60°C, and strong stirring and ultrasonic pretreatment were performed at 60°C for 30 min to obtain a mixed dispersion. The mixed dispersion was poured into a flask containing the lipid film, hydrated at 60°C for 30 min, and then ultrasonically treated at 350 W power for 10 min in an ice bath, filtered through a 0.45 μm filter membrane to obtain a liposome dispersion (the average particle size of the liposomes was 420 nm).

[0054] The formula amount of water was added into an emulsifying kettle and heated to 55°C. The formula amount of Rosa rugosa petals water, glycerin, butylene glycol, p-hydroxyacetophenone, propolis extract, Houttuynia cordata extract, Spilanthes acmella extract, Anthemis nobilis extract, Scutellaria baicalensis root extract, Paeonia suffruticosa root bark extract, and squalane, hexyldecanol, caprylic / capric triglyceride were sequentially added. The mixture was homogenized at 3000 r / min for 5 min, and then slowly cooled to 40°C to obtain a primary emulsion.

[0055] The liposome dispersion was added into the primary emulsion, and the mixture was stirred at 40°C and 500 r / min for 50 min. The pH value of the system was adjusted to 5.8 with a 10% citric acid solution. Finally, the mixture was filtered through a 200-mesh sieve, filled, and an anti-aging and whitening essential oil lotion was obtained.

[0056] Experimental Example 1: Stability Test The anti-aging and whitening essential oil lotions prepared in Examples 1-4 and Comparative Examples 1-2 were respectively placed in the following accelerated stability test conditions for 30 days: high temperature test: 40°C constant temperature box; light test: 4500±500 Lux light conditions. Before and after the 30-day test, samples of all the samples were taken for detection of the following indexes: (1) appearance change (color, transparency, presence or absence of stratification or precipitation); (2) pH value change (25°C); (3) viscosity change (25°C); (4) active ingredient retention rate (high performance liquid chromatography). The physical stability test results are shown in Table 1; the chemical stability test results are shown in Table 2.

[0057] Table 1: Physical stability test results

[0058] Table 2: Chemical stability test results (retention rate % after 30 days of high temperature test)

[0059] In Table 2, / represents that no test was performed.

[0060] As can be seen from Table 1, the samples of Examples 1-4 of the present application all showed excellent physical stability under high temperature and light conditions, with minimal changes in appearance, pH value and viscosity; while Comparative Example 1 (without packaging) and Comparative Example 2 (single packaging) showed obvious stratification, precipitation or color change. This shows that the double packaging system of the present application significantly improves the physical stability of the product. The chemical stability data in Table 2 further shows that after 30 days of high temperature storage, the retention rates of various active ingredients in the examples of the present application are all maintained at more than 93%, which is much higher than that of Comparative Example 1 (retention rate less than 75%) and Comparative Example 2 (retention rate between 82% and 86%). This shows that the double packaging system can effectively isolate and protect the easily degradable active ingredients, preventing their chemical inactivation.

[0061] Experimental Example 2: In vitro transdermal absorption efficiency evaluation A standard Franz diffusion cell apparatus was used. Fresh pig skin (thickness 0.8 mm) was used as the skin model, and phosphate buffer solution (containing 0.01 mol / L sodium dihydrogen phosphate and 0.01 mol / L disodium hydrogen phosphate, pH 7.4, 25°C, and containing 30% (v / v) ethanol) was filled under the skin (receiving cell). The samples of Example 1, Comparative Example 1, and Comparative Example 2 were selected for comparative testing. Equal amounts of the samples were uniformly applied to the surface of the pig skin (dose: 5 mg / cm 2 ), and the experiment was carried out under the conditions of 32°C and 400 rpm magnetic stirring. The receiving solution was withdrawn at 2, 4, 8, 12, and 24 hours, respectively, for analysis, and an equal amount of fresh receiving solution was replenished. The content of the three active ingredients in each withdrawn receiving solution was accurately determined using high-performance liquid chromatography, and the total amount of the three active ingredients that had accumulated in the receiving solution over 24 hours was calculated. The results of the transdermal absorption test are shown in Table 3.

[0062] Table 3: Transdermal absorption test results (unit: micrograms per square centimeter)

[0063] Table 3 shows that the 24-hour cumulative permeation amounts of the three active ingredients in Example 1 of the present application were significantly higher than those of Comparative Example 1 and Comparative Example 2. This result demonstrates that the synergistic delivery effect of liposomes and cyclodextrin inclusion complexes effectively promotes the transdermal absorption of active ingredients with different solubility characteristics.

[0064] Experimental Example 3: Evaluation of whitening efficacy (tyrosinase inhibitory activity) An in vitro chemical simulation method was used. Tyrosinase extracted from mushrooms was used as the enzyme source, and L-dopa (a precursor substance for melanin production) was used as the reaction substrate to construct a reaction system in a phosphate buffer solution at pH 6.8. The samples of Example 1, Comparative Example 1, and Comparative Example 2 were selected for comparative testing. The test samples were diluted with the buffer solution to the same concentration (10 mg / mL). The sample was mixed with the tyrosinase solution, and then the substrate L-dopa was added to start the reaction. The entire system was incubated at 37°C (simulating human body temperature) for 30 min. An enzyme marker was used to measure the absorbance of the reaction solution at a wavelength of 475 nm. The absorbance value was directly proportional to the amount of black melanin analog generated in the reaction. By comparing the absorbance of the sample group with that of the blank control group (without the sample), the inhibition rate of the sample on the activity of tyrosinase was calculated. The higher the inhibition rate, the better the potential whitening and lightening effect of the product. The tyrosinase inhibitory activity of different samples in Experimental Example 3 is shown in Figure 1 Figure 1 As can be seen from Table 4, the tyrosinase inhibitory ability of Example 1 was the strongest, with an inhibition rate close to 70%, and its effect was significantly higher than that of Comparative Example 1, which was not wrapped, and Comparative Example 2, which used a single wrapping.​

[0065] Experimental Example 4: Evaluation of antioxidant efficacy (DPPH free radical scavenging ability) A deep purple DPPH ethanol solution was used as the free radical source. Samples from Examples 1, 2, and 4 were selected for comparative testing with Comparative Examples 1 and 2. Each sample was diluted with solvent to the same test concentration (5 mg / mL). Equal amounts of sample solution and DPPH solution were mixed and allowed to stand in the dark for 30 min to allow the antioxidant components to fully react with the free radicals. The spectrophotometer was used to measure the absorbance of the reaction solution at a wavelength of 517 nm. After the purple DPPH free radicals were scavenged, the solution color became lighter and the absorbance decreased. By calculating the change in absorbance, the DPPH free radical scavenging rate of the sample was obtained, and a higher scavenging rate indicated stronger antioxidant ability. The DPPH free radical scavenging effect of different samples in Experimental Example 4 is shown in FIG. 1. As can be seen from FIG. 1, the DPPH free radical scavenging rates of the products of the embodiments of the double wrapping technology (1, 2, and 4) were all higher than 83%, exhibiting strong and consistent antioxidant ability, which was significantly better than Comparative Example 1 without wrapping and Comparative Example 2 with single wrapping. Figure 2 Figure 2 As can be seen from FIG. 1, the DPPH free radical scavenging rates of the products of the embodiments of the double wrapping technology (1, 2, and 4) were all higher than 83%, exhibiting strong and consistent antioxidant ability, which was significantly better than Comparative Example 1 without wrapping and Comparative Example 2 with single wrapping.

[0066] Therefore, the present application overcomes the technical bottlenecks of stability, transdermal permeability, and efficacy synergy of multi-active ingredient efficacy cosmetics from the principle by the innovative double synergistic wrapping system and the corresponding preparation method, and provides an essential oil lotion product with excellent comprehensive performance, anti-aging and whitening efficacy, and an efficient and reliable preparation method thereof.

[0067] Finally, it should be noted that the above examples are merely examples for clarity and do not limit the embodiments. For those of ordinary skill in the art, other different forms of changes or variations can be made on the basis of the above description. Here, it is not necessary or possible to exhaust all embodiments. The obvious changes or variations derived therefrom are still within the protection scope of the present application.​

Claims

1. An anti-aging and whitening essential oil lotion, characterized in that, It comprises an aqueous phase, an oil phase, and an encapsulation system; the encapsulation system comprises liposomes and cyclodextrin inclusion complexes. The liposomes encapsulate a first active ingredient, which includes at least one of Rosa damascena flower oil, meadowfoam seed oil, raspberry seed oil, tocopherol, and ascorbate tetraisopalmitate; the cyclodextrin inclusion complex encapsulates a second active ingredient, which includes at least one of 4-butylresorcinol, glycyrrhizin, and acetyl glucosamine.

2. The anti-aging and whitening essential oil lotion according to claim 1, characterized in that, The aqueous phase comprises water, glycerol, butanediol, and p-hydroxyacetophenone; the oil phase comprises squalane, hexyldecyl alcohol, and caprylic / capric triglyceride.

3. The anti-aging and whitening essential oil lotion according to claim 2, characterized in that, The aqueous phase also includes at least one of the following: Rosa damascena flower water, propolis extract, Houttuynia cordata extract, Portulaca oleracea extract, Chamomile extract, Scutellaria baicalensis root extract, and Paeonia suffruticosa root bark extract.

4. The anti-aging and whitening essential oil lotion according to claim 1, characterized in that, The wall material of the liposomes comprises at least one of hydrogenated lecithin and lecithin; the cyclodextrin is selected from hydroxypropyl-β-cyclodextrin.

5. The anti-aging and whitening essential oil lotion according to claim 1, characterized in that, In the encapsulation system, the weight ratio of the liposomes to the cyclodextrin inclusion complex is 1:(0.5-2).

6. The anti-aging and whitening essential oil lotion according to claim 5, characterized in that, By weight percentage, it includes the following components: The composition includes: Rosa damascena flower water 5-15%, glycerin 3-8%, butylene glycol 2-6%, p-hydroxyacetophenone 0.1-0.5%, propolis extract 0.1-2%, houttuynia cordata extract 0.01-0.1%, squalane 1-5%, hexyldecyl alcohol 0.5-2%, caprylic / capric triglyceride 1-5%, purslane extract 0.1-1%, golden chamomile extract 0.1-1%, scutellaria baicalensis root extract 0.1-1%, peony root bark extract 0.1-1%, encapsulation system 1-10%, and the balance being water.

7. A method for preparing the anti-aging and whitening essential oil lotion according to any one of claims 1-6, characterized in that, Includes the following steps: S1. Prepare liposome dispersions and cyclodextrin inclusion complexes separately; The aqueous phase component and the oil phase component are mixed and emulsified to obtain the primary emulsion; S2. The liposome dispersion, cyclodextrin inclusion complex and primary emulsion are mixed to obtain the anti-aging and whitening essential oil lotion.

8. The method according to claim 7, characterized in that, In S1, the process of preparing the liposome dispersion includes: dissolving the wall material of the liposome in an organic solvent, and forming a lipid membrane after removing the solvent; mixing the first active ingredient with a buffer solution, and then hydrating and sonicating the lipid membrane to obtain the liposome dispersion; The process of preparing cyclodextrin inclusion complex includes: dissolving cyclodextrin in water, mixing it with a second active ingredient, and then sequentially performing a stirring reaction and ball milling treatment. After filtration and drying, the cyclodextrin inclusion complex is obtained.

9. The method according to claim 7, characterized in that, In S1, the mixing temperature is 50-60℃; the emulsification speed is 2500-3500 r / min, and the time is 3-7 min.

10. The method according to claim 7, characterized in that, In S2, the mixing temperature is 35-45℃, the rotation speed is 300-700 r / min, and the time is 35-60 min; After mixing, the process also includes adjusting the pH value to 5.0-6.5 using a citric acid solution.

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