Propionibacterium acnes resisting composition, acne removing composition and application thereof

Abstract

The invention belongs to the field of new daily chemical materials, and discloses an anti-propionibacterium acnes composition, which comprises a radix sophorae flavescentis extract and colloidal sulfur, and the weight ratio of the radix sophorae flavescentis extract to the colloidal sulfur is (0.5-1): (5-10). Meanwhile, the invention also discloses an acne-removing composition, which is prepared from the radix sophorae flavescentis extract, colloidal sulfur and palmitoyl tetrapeptide-7, the weight ratio of the radix sophorae flavescentis extract to the palmitoyl tetrapeptide-7 to the colloidal sulfur is (0.5-1): (0.01-0.05): (5-10); according to the propionibacterium acnes resisting composition, the radix sophorae flavescentis extract and the colloidal sulfur are adopted as active ingredients, and the radix sophorae flavescentis extract has a certain antibacterial ability and can effectively promote the antibacterial performance of the colloidal sulfur to play. The acne-removing composition can effectively control acnes, remove acnes and eliminate pockmarks. Meanwhile, the invention also provides application of the composition.

Description

Technical Field

[0001] This invention relates to the field of new materials for daily chemical products, and in particular to an anti-acne Propionibacterium acnes composition, an acne-removing composition, and their applications. Background Technology

[0002] Currently, commercially available products are mainly divided into ① products containing acids such as salicylic acid and azelaic acid, which kill acne bacteria and exfoliate the skin to achieve acne removal effects. However, these products are highly irritating and can easily damage the skin again; ② preservatives listed in the preservative catalog, mainly o-cymene-5-ol. However, consumers in the market do not like products containing preservatives.

[0003] Commercially available products for treating acne scars and pits work by replenishing collagen, but they cannot be used in the same way as acid-based acne treatments. Acidic ingredients can damage the structure of collagen and affect its effectiveness.

[0004] In patent application CN113576975A, entitled "An Easily Absorbable Anti-aging Composition and Its Preparation Method and Application," an easily absorbable anti-aging composition is disclosed, comprising: 1-5 parts of retinyl palmitate, 1-5 parts of acetyl hexapeptide-8, and palmitoyl tetrapeptide-7. 1-5 parts, trehalose 1-10 parts, sodium lactate 1-5 parts, tocopherol 1-5 parts, sodium hyaluronate 0.1-1 parts, horse chestnut seed extract 1-6 parts, sophora flavescens root extract 1-4 parts, carnosine 0.1-1 parts, creatine 0.1-1 parts, polyglutamic acid 0.1-1 parts, xanthan gum 0.1-0.5 parts, compound preservative 0.01-1 parts, water 30-90 parts; it further points out that acetyl hexapeptide-8 and palmitoyl tetrapeptide-7, as other anti-aging active ingredients with synergistic effects with retinyl palmitate, are polypeptide components that promote collagen production, alleviate fine lines and wrinkles, and enhance the anti-aging effect of retinyl palmitate. Combined with beneficial ingredients such as trehalose, sodium hyaluronate, horse chestnut seed extract, and sophora flavescens root extract, the anti-aging effect of the main anti-aging active ingredients is enhanced, addressing signs of aging such as wrinkles and loss of elasticity in the skin.

[0005] As can be seen from this scheme, it uses retinyl palmitate as the core ingredient and palmitoyl tetrapeptide-7, sophora flavescens root extract and other raw materials as promoters to achieve the effects of anti-aging and wrinkle removal.

[0006] However, the above solutions have obvious shortcomings in controlling acne and eliminating acne scars.

[0007] Therefore, the technical problem to be solved in this case is: how to effectively remove acne and eliminate acne scars. Summary of the Invention

[0008] The purpose of this invention is to provide an anti-Propionibacterium acnes composition, which uses Sophora flavescens root extract and colloidal sulfur as active ingredients. Sophora flavescens root extract itself has a certain antibacterial ability, and at the same time, it can effectively promote the antibacterial performance of colloidal sulfur.

[0009] Meanwhile, the present invention also provides an acne-removing composition and its application; the acne-removing composition can effectively control acne, remove acne and eliminate acne scars.

[0010] The specific solution of the present invention is as follows:

[0011] An anti-acne Propionibacterium acnes composition comprising Sophora flavescens root extract and colloidal sulfur, wherein the weight ratio of Sophora flavescens root extract to colloidal sulfur is 0.5~1:5~10.

[0012] The composition was tested using Propionibacterium acnes and it was found that both Sophora flavescens root extract and colloidal sulfur have independent anti-acne abilities. At the same time, the combination of the two showed a relatively obvious synergistic effect.

[0013] Meanwhile, the present invention also discloses an acne-removing composition, comprising Sophora flavescens root extract, colloidal sulfur, and palmitoyl tetrapeptide-7; wherein the weight ratio of Sophora flavescens root extract, palmitoyl tetrapeptide-7, and colloidal sulfur is 0.5~1:0.01~0.05:5~10.

[0014] The synergistic mechanism of this composition is as follows:

[0015] Sophora flavescens root extract is an alkaloid extracted from the plant Sophora flavescens, belonging to the genus Sophora in the legume family. Its main components include matrine, isoflavone, and flavonoids. These alkaloids can inhibit the activity of Propionibacterium acnes and achieve anti-inflammatory effects by inhibiting the activation of the TLR4 / MyD88 / NF-κB signaling pathway and the NLRP3 inflammasome.

[0016] Colloidal sulfur: Sulfur encapsulated in gum arabic. When sulfur comes into contact with the skin, it is partially converted into sulfides such as hydrogen sulfide and pentasulfuric acid. It not only has bactericidal and insecticidal effects, but also stimulates pores to open, expelling excess sebum that clogs pores. It also facilitates the penetration of active ingredients into hair follicles and sebaceous glands to exert their effects directly.

[0017] Palmitoyl Tetrapeptide-7: Palmitoyl Tetrapeptide-7 reduces IL-6 secretion by acting on keratinocytes, thereby reducing inflammation and making the skin firmer, smoother, and more elastic. Simultaneously, it acts as a cellular messenger to stimulate the regeneration of collagen fibers in the skin, and stimulates the synthesis of collagen (type I, II, and IV) and elastin in the dermis.

[0018] This composition can inhibit the growth of Propionibacterium acnes from multiple directions, including different cellular pathways and antibacterial effects, and can also soothe and reduce inflammation. Volunteer testing showed that it effectively resolved problems such as acne, acne marks, and acne pits, demonstrating excellent acne-removing efficacy.

[0019] In the above-mentioned acne-removing composition, the weight ratio of Sophora flavescens root extract, palmitoyl tetrapeptide-7, and colloidal sulfur is 0.5~1:0.02:5~10.

[0020] In addition, the present invention also discloses the use of the acne-removing composition described above in the preparation of hair cosmetics.

[0021] Finally, the present invention also discloses a cosmetic comprising the anti-dandruff composition as described above.

[0022] In the aforementioned cosmetics, the content of the anti-dandruff composition is 0.51~10.55wt%.

[0023] This application has at least the following beneficial effects:

[0024] The anti-Propionibacterium acnes composition of the present invention has a significant synergistic effect in combating Propionibacterium acnes.

[0025] The acne-removing composition of the present invention can inhibit the growth of Propionibacterium acnes and also soothe and reduce inflammation. Volunteer testing showed that it effectively resolved problems such as acne, acne marks, and acne pits, demonstrating excellent acne-removing efficacy. Attached Figure Description

[0026] Figure 1 These are photos taken before the experiment to evaluate the anti-inflammatory effect of Example 1;

[0027] Figure 2 These are photos taken after the experiment evaluating the anti-inflammatory effect of Example 1;

[0028] Figure 3 These are photos taken before the experiment to evaluate the anti-inflammatory effect of Example 2;

[0029] Figure 4 These are photos taken after the experiment evaluating the anti-inflammatory effect of Example 2;

[0030] Figure 5 These are photos taken before the experiment to evaluate the skin smoothness effect in Example 1;

[0031] Figure 6 These are photos taken after the experiment evaluating the skin smoothness effect of Example 1;

[0032] Figure 7 These are photos taken before the experiment to evaluate the skin smoothness effect in Example 2;

[0033] Figure 8 These are photos taken after the experiment evaluating the skin smoothness effect in Example 2.

[0034] Specific implementation methods

[0035] The present invention will now be clearly and completely described in conjunction with embodiments thereof. It should be noted that, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0036] Raw material source:

[0037] Sophora flavescens root extract: Shanghai Xier Biotechnology Co., Ltd., Sophora flavescens extract, active ingredient content 98wt%;

[0038] Colloidal sulfur: Guangzhou Wanlu Chemical Co., Ltd., Sulfidal™ Colloidal Sulfur Cosmetic Grade, active ingredient content 100wt%;

[0039] Palmitoyl Tetratide-7: cBioMey™ Pal-Tetratide-7 (palmitoyl tetratide-7), active ingredient content 100wt%, produced by Hezhimei (Guangzhou) Biotechnology Co., Ltd.

[0040] Part 1: Antibacterial Experiment of Propionibacterium acnes

[0041] Propionibacterium acnes is the main bacterium affecting the occurrence of acne, and there is usually a very significant abnormal proliferation of Propionibacterium acnes in acne patients.

[0042] Relevance of test methods: This test evaluates the acne treatment products prepared in Examples 1, 2 and Comparative Examples 1-4 by referring to the 5.1.4 inhibition zone test recorded in the standard "WS / T 650-2019 Evaluation Method for Antibacterial and Bacteriostatic Effects".

[0043] The formulations for the embodiments and comparative examples in this section can be found in Table 1;

[0044] Table 1 Formulation Table Unit: wt%

[0045] Group raw material Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 A Deionized water To100 To100 To100 To100 To100 A U20 0.5 0.5 0.5 0.5 0.5 A 1,3-Butanediol 8 8 8 8 8 A EDTA-2Na 0.05 0.05 0.05 0.05 0.05 B Arginine 0.5 0.5 0.5 0.5 0.5 B Deionized water 3 3 3 3 3 C Fresh Ketone 0.4 0.4 0.4 0.4 0.4 C Hexanediol 0.4 0.4 0.4 0.4 0.4 C 1,3-Butanediol 2 2 2 2 2 D Sophora flavescens root extract 0.5 1 0 0.5 0 D Deionized water 5 5 5 5 5 E TW-20 3 3 3 3 3 E Palmitoyl Tetrapeptide-7 0.02 0.02 0.02 0.02 0.02 H colloidal sulfur 10 5 10 0 0

[0046] The preparation method of the formula in Table 1 above is as follows:

[0047] 1. Accurately weigh phase A raw material at room temperature and add it to a vacuum emulsifying pot. Homogenize until completely uniform and free of particles, then stir and heat to 80-85℃;

[0048] 2. Cool to 70-75℃, add the pre-dissolved transparent B-phase raw material, stir evenly, apply vacuum, and continue;

[0049] 3. Cool to 40-45℃, add the preheated (50-60℃) dissolved and transparent C-phase raw material, and stir until homogeneous;

[0050] 4. Add the pre-dissolved, transparent D and E phase raw materials and stir until homogeneous;

[0051] 5. Add the H phase raw material, homogenize at low speed for 1-2 minutes, and stir until completely homogeneous.

[0052] The results of the antibacterial experiment are shown in Table 2.

[0053] Table 2 Results of antibacterial experiment

[0054] Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Average diameter of inhibition zone (mm) 17.64 12.36 9.15 6.31 5.12

[0055] Results analysis:

[0056] As shown in Table 2, Examples 1 and 2, and Comparative Examples 1 and 2 all exhibited antibacterial effects, while Comparative Example 3 showed no antibacterial effect. Example 1 demonstrated the best antibacterial effect. The data from Example 1, Comparative Examples 1 and 2 confirm that Sophora flavescens root extract and colloidal sulfur can synergistically enhance the antibacterial effect.

[0057] Part Two: Volunteer Experiment

[0058] 1. Evaluation of anti-inflammatory effect

[0059] Volunteer Information Reference Table 3;

[0060] Table 3 Volunteer Information Form

[0061] Volunteer ID Volunteer abbreviation Volunteer ID Volunteer abbreviation Volunteer ID Volunteer abbreviation 1 LY 11 WH 21 LJY 2 GXL 12 LQJ 22 CHQ 3 FZJ 13 YZ 23 SRW 4 CJF 14 JJF 24 LW 5 LST 15 WDX 25 HWZ 6 HGQ 16 TXJ 26 OYH 7 HXY 17 FQY 27 LJY 8 XSS 18 YH 28 FWM 9 TXL 19 WHB 29 HCH 10 PLH 20 YWR 30 QMY

[0062] The experimental groupings are shown in Table 4.

[0063] Table 4 Experimental Grouping Table

[0064] Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Volunteer ID 1-5 6-10 11-15 16-20 20-25 26-30

[0065] Experimental Methods: When the skin is in an inflammatory state, the area of ​​redness on the skin will increase. The anti-inflammatory effects of the acne treatment products prepared in the examples and comparative examples can be evaluated using the VISIA instrument and the Image Pro Plus method.

[0066] Rate of change = (test - initial) / initial * 100%

[0067] 2. Evaluation of skin smoothness effect

[0068] Volunteer information: Same as above

[0069] Experimental grouping: Same as above

[0070] Experimental method: After acne subsides, the skin smoothness will be lower than before. The skin smoothness effect of the acne treatment products prepared in the examples and comparative examples can be evaluated by using a VISIA instrument with a three-dimensional observation mirror.

[0071] The formulations of the embodiments and comparative examples involved in this section are referenced in Table 5;

[0072] Table 5 Formulation Table Unit: wt%

[0073] Group raw material Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 A Deionized water To100 To100 To100 To100 To100 To100 A U20 0.5 0.5 0.5 0.5 0.5 0.5 A 1,3-Butanediol 8 8 8 8 8 8 A EDTA-2Na 0.05 0.05 0.05 0.05 0.05 0.05 B Arginine 0.5 0.5 0.5 0.5 0.5 0.5 B Deionized water 3 3 3 3 3 3 C Fresh Ketone 0.4 0.4 0.4 0.4 0.4 0.4 C Hexanediol 0.4 0.4 0.4 0.4 0.4 0.4 C 1,3-Butanediol 2 2 2 2 2 2 D Sophora flavescens root extract 0.5 1 0 0.5 0 0.5 D Deionized water 5 5 5 5 5 5 E TW-20 3 3 3 3 3 3 E Palmitoyl Tetrapeptide-7 0.02 0.02 0.02 0.02 0.02 0 H colloidal sulfur 10 5 10 0 0 10

[0074] The preparation method is described in Part 1.

[0075] The results of the anti-inflammatory effect evaluation and the skin smoothness effect evaluation are shown in Table 6.

[0076] Table 6 Evaluation Results

[0077] Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Red zone area change rate (%) -24.56 -24.17 -13.34 -17.72 -2.49 -17.24 Change rate in skin smoothness (%) -11.26 -10.88 -10.75 -11.37 -6.84 -6.55

[0078] The red area change rate photographs of Examples 1 and 2 can be referenced. Figures 1 to 4 The skin smoothness change rate in Examples 1 and 2 can be referenced. Figures 5 to 8 ;

[0079] Results analysis:

[0080] As shown in Table 4, the soothing effect, from greatest to least, is: Example 1 ≈ Example 2 > Comparative Example 2 ≈ Comparative Example 4 > Comparative Example 1 > Comparative Example 3. Specifically, the data from Example 1 and Comparative Example 2 indicate that colloidal sulfur itself has no anti-inflammatory effect, but it is speculated that it can promote the penetration of anti-inflammatory components to enhance their anti-inflammatory efficacy. The data from Example 1 and Comparative Example 1 show that palmitoyl tetrapeptide-7 can enhance its anti-inflammatory effect. The skin smoothing effect, from greatest to least, is: Example 1 ≈ Comparative Example 2 > Example 2 ≈ Comparative Example 1 > Comparative Example 4 ≈ Comparative Example 3. Specifically, the data from Example 1 and Comparative Example 4 show that palmitoyl tetrapeptide-7 has the effect of smoothing skin texture.

[0081] Based on the data above, we can draw the following conclusions:

[0082] 1. Palmitoyl tetrapeptide-7 is absolutely important in smoothing skin texture;

[0083] 2. Palmitoyl tetrapeptide-7 plays a very important role in soothing human trials;

[0084] 3. When either colloidal sulfur or Sophora flavescens root extract is combined with palmitoyl tetrapeptide-7, it does not produce a particularly good soothing effect. However, when all three are used in combination, they produce a very significant soothing effect. This indicates that the three have a clear synergistic effect in soothing the skin.

[0085] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. An anti-acne Propionibacterium acnes composition, characterized in that, It includes Sophora flavescens root extract and colloidal sulfur, wherein the weight ratio of Sophora flavescens root extract to colloidal sulfur is 0.5~1:5~10.

2. An acne-removing composition, characterized in that, It includes Sophora flavescens root extract, colloidal sulfur, and palmitoyl tetrapeptide-7; the weight ratio of Sophora flavescens root extract, palmitoyl tetrapeptide-7, and colloidal sulfur is 0.5~1:0.01~0.05:5~10.

3. The acne-removing composition according to claim 2, characterized in that, The weight ratio of the Sophora flavescens root extract, palmitoyl tetrapeptide-7, and colloidal sulfur is 0.5~1:0.02:5~10.

4. Use of the acne-removing composition as described in claim 2 or 3 in the preparation of hair cosmetics.

5. A cosmetic product, characterized in that, It contains the anti-dandruff composition as described in claim 2 or 3.

6. The cosmetic product according to claim 5, characterized in that, The content of the anti-dandruff composition in the cosmetic product is 0.51~10.55wt%.

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