Method for extracting active substances in traditional Chinese medicine formula for soap

Abstract

The invention discloses a method for extracting active substances of a traditional Chinese medicine formula for soap, and belongs to the technical field of extraction of active substances of traditional Chinese medicines. According to the invention, a Chinese patent medicine Huidoqing capsule is taken as a prototype, and active substances in a traditional Chinese medicinal material formula (containing radix sophorae flavescentis, cortex dictamni and the like) are extracted by adopting a deep eutectic solvent (DES) and utilizing an ultrasonic-assisted technology. The taurine-urea eutectic solvent prepared by the method has the best extraction effect on total flavonoids and alkaloids in a traditional Chinese medicine formula. On the basis, the medicated soap is prepared from the traditional Chinese medicine formula extracting solution, and the good use evaluation is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to a method for extracting active substances of traditional Chinese medicine prescriptions for soap, belonging to the field of traditional Chinese medicine active substance extraction technology. BACKGROUND

[0002] The study of active ingredients of traditional Chinese medicine is an important bridge connecting traditional medicine and modern pharmacology. Among them, flavonoids and alkaloids have become the focus due to their diverse structures and extensive biological activities.

[0003] Flavonoids are widely distributed in medicinal materials such as Huangqi, Huaihua, and Gegen, and their basic skeleton is C6 C3 C6 structure, which is substituted by hydroxyl, glycoside, etc. to form isoflavones, flavonols, etc. The polyphenol hydroxyl group gives it strong antioxidant capacity, which can scavenge free radicals, inhibit lipid peroxidation, and enhance cell defense through signal pathway regulation. This kind of component has significant effect on anti-inflammatory and anti-allergic, such as Huangqin relieving respiratory inflammation and Gegen protecting cardiovascular endothelium.

[0004] Alkaloids are characterized by nitrogen-containing heterocycles, found in medicinal materials such as Huanglian, Mahuang, and Kushen, and have more targeted pharmacological activities. Caffeine can refresh the mind by blocking adenosine receptors; morphine activates opioid receptors to achieve strong analgesia; quinine and its derivative chloroquine are classic antimalarials by inhibiting malaria nucleic acid replication; capsaicin is developed as a local analgesic patch. The application of alkaloids has been expanded to the fields of medicine, agriculture, and scientific research.

[0005] Current extraction techniques for active substances are simple and low-cost, suitable for water-soluble components (such as polysaccharides and flavonoids), but have low efficiency for lipid-soluble components, and some active components are prone to oxidation and hydrolysis. Organic solvents such as ethanol can extract lipid-soluble components, but there are solvent residues and environmental pressure.

[0006] Traditional Chinese medicine theory believes that the combination of Radix Rehmanniae, Angelica sinensis, Sophora flavescens, Dictamnus dasycarpus, Salvia miltiorrhiza, Glycyrrhiza uralensis, Scrophularia ningpoensis, and Alpinia oxyphylla has the effects of clearing heat and blood, expelling wind and dampness, and promoting blood circulation and nourishing blood. Components such as Dictamnus dasycarpus, Sophora flavescens, and Huangqin may have potential antipruritic effects.

[0007] Therefore, it is of great practical and economic value to develop a method for efficiently extracting active substances from traditional Chinese medicine prescriptions. SUMMARY

[0008] To solve the above problems, the present application takes Shiduqing capsule as the prototype, and uses a deep eutectic solvent (DES) to extract active substances in a traditional Chinese medicine formula (containing Sophora flavescens, Dictamnus dasycarpus, etc.) by using ultrasonic-assisted technology. The taurine-urea deep eutectic solvent prepared in the present application has the best extraction effect on total flavonoids and alkaloids in the traditional Chinese medicine formula. On this basis, the present application uses the traditional Chinese medicine formula extract to prepare a medicinal soap, and the medicinal soap prepared has good use evaluation.

[0009] The first object of the present application is to provide a method for extracting active substances in a traditional Chinese medicine formula, comprising the steps of: mixing the taurine-urea deep eutectic solvent with the traditional Chinese medicine component powder, ultrasonic extraction, centrifugation, and filtration to obtain a traditional Chinese medicine formula extract containing active substances; The traditional Chinese medicine component powder is a mixture of Rehmannia glutinosa, Angelica sinensis, Sophora flavescens, Dictamnus dasycarpus, Salvia miltiorrhiza, Glycyrrhiza uralensis, Scrophularia ningpoensis, and Smilax glabra, which are ground into powder; and the active substances are total flavonoids and alkaloids. The preparation method of the taurine-urea deep eutectic solvent is as follows: Mix taurine and urea at a molar ratio of 1:4-6, heat, and obtain the taurine-urea deep eutectic solvent.

[0010] In one embodiment, the heating is at 75-85℃ for 1.5-2.5 h.

[0011] In one embodiment, after mixing taurine and urea, water is added until the water content reaches 10-30%.

[0012] In one embodiment, the traditional Chinese medicine component powder is a mixture of Rehmannia glutinosa, Angelica sinensis, Sophora flavescens, Dictamnus dasycarpus, Salvia miltiorrhiza, Glycyrrhiza uralensis, Scrophularia ningpoensis, and Smilax glabra, which are mixed at a mass ratio of 8:8:8:8:4:2:1:1 and ground into powder.

[0013] In one embodiment, the dosage ratio of the traditional Chinese medicine component powder to the taurine-urea deep eutectic solvent is 1 g:25-40 g.

[0014] In one embodiment, the ultrasonic extraction is at 40-60℃ for 30-50 min.

[0015] In one embodiment, the taurine-choline chloride (molar ratio 1:2) deep eutectic solvent, lactic acid-urea (molar ratio 1:2) deep eutectic solvent, lactic acid-urea (molar ratio 1:2) deep eutectic solvent, lactic acid and glucose (molar ratio 5:1) deep eutectic solvent, and propylene glycol-malic acid (molar ratio 3:1) deep eutectic solvent can also be used to extract the traditional Chinese medicine component powder.

[0016] The second object of the present application is to provide a traditional Chinese medicine formula extract prepared by any of the above methods.

[0017] A third object of the present application is to provide a product containing the traditional Chinese medicine formula extract described above, which is a washing and protecting product.

[0018] A fourth object of the present application is to provide a medicinal soap containing the traditional Chinese medicine formula extract described above, and the preparation method is as follows: mixing a soap base, stearic acid, cocamide propyl betaine and glycerol, adding the traditional Chinese medicine formula extract, mixing uniformly, pouring into a mold, cooling, and obtaining the medicinal soap.

[0019] In an embodiment, the amount ratio of the soap base, stearic acid, cocamide propyl betaine, glycerol and the traditional Chinese medicine formula extract is 80-100 g: 1-5 g: 2-6 mL: 0.5-2 g: 1-5 mL.

[0020] In an embodiment, the soap base, stearic acid, cocamide propyl betaine and glycerol are mixed, and after adding the traditional Chinese medicine formula extract, heating and mixing uniformly at 50-60℃.

[0021] A fifth object of the present application is to provide the use of the traditional Chinese medicine formula extract described above in the preparation of a washing and protecting product.

[0022] A sixth object of the present application is to provide a method for improving the extraction effect of active substances in a traditional Chinese medicine formula, which uses taurine-urea eutectic solvent extraction, and the steps include: Mixing taurine-urea eutectic solvent with traditional Chinese medicine component powder, ultrasonic extraction, centrifugation, filtration, and obtaining a traditional Chinese medicine formula extract containing active substances; The traditional Chinese medicine component powder is obtained by mixing and pulverizing radix rehmanniae, angelica sinensis, sophora flavescens, cortex dictamni, salvia miltiorrhiza, glycyrrhiza uralensis, radix scrophulariae and smilax glabra; and the active substances are total flavonoids and alkaloids. The preparation method of the taurine-urea eutectic solvent is as follows: Mixing taurine and urea according to a molar ratio of 1:4-6, heating, and obtaining taurine-urea eutectic solvent.

[0023] In an embodiment, the heating is at 75-85℃ for 1.5-2.5 h.

[0024] In an embodiment, after mixing taurine and urea, water is mixed therewith until the water content reaches 10-30%.

[0025] In an embodiment, the traditional Chinese medicine formula powder is obtained by mixing and pulverizing radix rehmanniae, angelica sinensis, sophora flavescens, cortex dictamni, salvia miltiorrhiza, glycyrrhiza uralensis, radix scrophulariae and smilax glabra according to a mass ratio of 8:8:8:8:4:2:1:1.

[0026] In an embodiment, the amount ratio of the traditional Chinese medicine component powder and the taurine-urea eutectic solvent is 1 g: 25-40 g.

[0027] In an embodiment, the ultrasonic extraction is 40-60℃ ultrasonic extraction for 30-50 min.

[0028] Advantages of the present application The present application takes Shiduqing capsule as a prototype, and uses a deep eutectic solvent (DES) to extract active substances in a traditional Chinese medicine prescription (containing Sophora flavescens, Dictamnus dasycarpus, etc.) by using ultrasonic auxiliary technology. The taurine-urea deep eutectic solvent prepared in the present application has the best extraction effect on total flavonoids and alkaloids in the traditional Chinese medicine prescription.

[0029] Specifically: The present application uses taurine-urea deep eutectic solvent to extract the traditional Chinese medicine prescription, and the total flavonoids in the extract reach 12.25 mg / g, and the alkaloids reach 54.76 mg / g. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 is a content comparison chart of active ingredient extract solutions of the traditional Chinese medicine prescription prepared by examples 1-6, comparative examples 1-2 and water extraction; Figure 2 is feedback after the tester uses the soap. DETAILED DESCRIPTION

[0031] The preferred embodiments of the present application are described below, and it should be understood that the embodiments are used to better explain the present application, and are not used to limit the present application.

[0032] Raw materials used in the examples: Taurine is purchased from the National Pharmaceutical Group Chemical Reagent Co., Ltd., and the CAS number is 107-35-7; Urea is purchased from Shanghai Haohong Biological Medicine Technology Co., Ltd., and the CAS number is 57-13-6; Choline chloride is purchased from Shanghai Haohong Biological Medicine Technology Co., Ltd., and the CAS number is 67-48-1; Glucose is purchased from Shanghai Haohong Biological Medicine Technology Co., Ltd., and the CAS number is 50-99-7; Lactic acid is purchased from Shanghai Maikelin Biochemical Technology Co., Ltd., and the CAS number is 50-21-5; Propylene glycol is purchased from Shanghai Haohong Biological Medicine Technology Co., Ltd., and the CAS number is 57-55-6; Malic acid is purchased from Shanghai Maikelin Biochemical Technology Co., Ltd., and the CAS number is 617-48-1; Rehmannia glutinosa, Angelica sinensis, Sophora flavescens, Dictamnus dasycarpus, Salvia miltiorrhiza, Glycyrrhiza uralensis, Scrophularia ningpoensis, and Smilax glabra are all from Miluo City Yumeng Pharmaceutical Co., Ltd. Example 1: Preparation of a deep eutectic solvent Preparation of taurine-urea deep eutectic solvent, the steps are as follows: Taurine, urea were mixed according to a molar ratio of 1:5, then mixed with water to reach a water content of 20% w / w, heated at 80°C for 2 h, and a uniform transparent liquid, i.e. taurine-urea deep eutectic solvent, was obtained.

[0033] Example 2: Preparation of deep eutectic solvent On the basis of Example 1, choline chloride was used to replace urea according to a molar ratio of 1:2, heated at 80°C for 2 h, and taurine-choline chloride deep eutectic solvent was prepared.

[0034] Example 3: Preparation of deep eutectic solvent On the basis of Example 1, glucose was used to replace urea according to a molar ratio of 1:2, heated at 80°C for 2 h, and taurine-glucose deep eutectic solvent was prepared.

[0035] Example 4: Preparation of deep eutectic solvent On the basis of Example 1, lactic acid was used to replace taurine according to a molar ratio of 1:2, heated at 80°C for 2 h, and lactic acid-urea deep eutectic solvent was prepared.

[0036] Example 5: Preparation of deep eutectic solvent On the basis of Example 1, lactic acid and glucose (molar ratio 5:1) were used, heated at 80°C for 2 h, and lactic acid-glucose deep eutectic solvent was prepared.

[0037] Example 6: Preparation of deep eutectic solvent On the basis of Example 1, propylene glycol and malic acid (molar ratio 3:1) were used, heated at 50°C for 2 h, and propylene glycol-malic acid deep eutectic solvent was prepared.

[0038] Comparative Example 1: Change of ratio On the basis of Example 1, the molar ratio of taurine to urea was changed to 1:3, and the remaining steps were kept unchanged, and taurine-urea deep eutectic solvent was prepared.

[0039] Comparative Example 2: Change of ratio On the basis of Example 1, the molar ratio of taurine to urea was changed to 1:7, and the remaining steps were kept unchanged, and taurine-urea deep eutectic solvent was prepared.

[0040] Example 7: Application of deep eutectic solvent in extraction of active components in traditional Chinese medicine prescriptions The eutectic solvent prepared in Examples 1-6 was detected for its application in extracting active components in traditional Chinese medicine formula. The traditional Chinese medicine formula was composed of eight medicinal materials, namely, radix rehmanniae, angelica sinensis, sophora flavescens, cortex ponciri, salvia miltiorrhiza, glycyrrhiza uralensis, radix scrophulariae, and smilax glabra, which were mixed in a mass ratio of 8:8:8:8:4:2:1:1, ground, and sieved through a 40-mesh sieve to obtain a powder of the eight medicinal materials.

[0041] 1 g of the powder of the traditional Chinese medicine formula was taken and mixed with 32.5 g of different eutectic solvents, and then ultrasonically extracted at 50°C for 40 min. After extraction, the mixture was centrifuged and filtered to obtain an extract, which was used as a control. The total flavonoid content and alkaloid content in the extract were detected, and the results are shown in Table 1.

[0042] Table 1: Total flavonoid and alkaloid contents

[0043] The results show that the extraction effect of the traditional Chinese medicine formula using the taurine-urea eutectic solvent in Example 1 is significantly better than that in Examples 2-6 and the water extraction method, and the alkaloid content is significantly increased. By comparing Example 1 with Comparative Examples 1-2, it can be seen that changing the ratio of taurine to urea greatly affects the extraction effect of active components.

[0044] Example 8: Application of eutectic solvent extract in preparing medicinal soap 1. Preparation of medicinal soap The taurine-urea extract of the traditional Chinese medicine formula in Example 7 was used to prepare medicinal soap, and the steps were as follows: 90 g of a soap base, 3 g of stearic acid, 4 mL of cocamide propyl betaine, and 1.0 g of glycerol were weighed into a beaker, and then 2 mL of the taurine-urea extract of the traditional Chinese medicine formula was slowly added. The mixture was heated in a water bath at 60°C. After the soap base was dissolved, the mixture was continuously heated and gently stirred until it was completely mixed and uniform. The heated mixture was then poured into a mold and cooled in a 4°C refrigerator to form a medicinal soap.

[0045] 2. Evaluation of medicinal soap use A systematic trial test was conducted by recruiting social groups, and a quantitative questionnaire (such as skin moisturizing degree, irritation, and itching relief effect) was used to record the actual performance of the medicinal soap. First, the DES solvent was added to the soap formula and evaluated for use to explore whether the use of lactic acid-based DES solvent would make the soap irritating. After determining its safety, the extract was added to the soap to evaluate its use experience.

[0046] Table 2: Safety evaluation of soap use

[0047] The feedback on the use of medicinal soap is as follows: Figure 2As shown, the results show that the basic component in the soap base can well neutralize the lactic acid-based DES solvent added by us, and the user does not feel obvious irritation, which proves that the added component can be safely used in the soap.

[0048] Table 3 Feedback of the testers after using the medicinal soap

[0049] The evaluation results in the above table are statistically evaluated using a 5-point system for the use of the medicinal soap, and the scores from 5 to 1 are very good, good, general, poor, and very poor, respectively. The results show that the prepared medicinal soap has good use evaluation.

[0050] Although the present application has been disclosed with the preferred embodiments as above, it is not intended to limit the present application, and any person skilled in the art can make various modifications and modifications without departing from the spirit and scope of the present application, and therefore the protection scope of the present application should be defined by the claims.

Claims

1. A method for extracting active substances from traditional Chinese medicine formulas, characterized in that, Including the following steps: The taurine-urea eutectic solvent was mixed with the powder of traditional Chinese medicine components, and the mixture was extracted by ultrasonication, centrifuged, and filtered to obtain the extract of traditional Chinese medicine formula containing active substances. The powdered traditional Chinese medicine components are obtained by mixing and pulverizing Rehmannia glutinosa, Angelica sinensis, Sophora flavescens, Dictamnus dasycarpus, Salvia miltiorrhiza, Glycyrrhiza uralensis, Scutellaria baicalensis, and Smilax glabra; the active substances are total flavonoids and alkaloids. The preparation method of the taurine-urea eutectic solvent is as follows: Taurine and urea are mixed in a molar ratio of 1:4 to 6 and heated to obtain a taurine-urea eutectic solvent.

2. The method according to claim 1, characterized in that, Heat at 75~85℃ for 1.5~2.5 h; mix taurine and urea, then mix with water until the water content reaches 10~30%.

3. The method according to claim 1, characterized in that, The powdered Chinese herbal medicine components are obtained by mixing and pulverizing Rehmannia glutinosa, Angelica sinensis, Sophora flavescens, Dictamnus dasycarpus, Salvia miltiorrhiza, Glycyrrhiza uralensis, Scutellaria baicalensis, and Smilax glabra in a mass ratio of 8:8:8:8:4:2:1:

1.

4. The method according to claim 1, characterized in that, The mass ratio of the Chinese herbal medicine powder to the taurine-urea eutectic solvent is 1 g: 25~40 g.

5. The method according to claim 1, characterized in that, Ultrasonic extraction was performed at 40-60℃ for 30-50 minutes.

6. The herbal extract prepared by the method according to any one of claims 1 to 5.

7. A product characterized in that, The product contains the extract of the traditional Chinese medicine formula as described in claim 6, and the product is a shampoo and body care product.

8. A medicated soap, characterized in that, The medicated soap contains the traditional Chinese medicine formula extract as described in claim 6. The preparation method is as follows: soap base, stearic acid, cocamidopropyl betaine, and glycerol are mixed, the traditional Chinese medicine formula extract is added, the mixture is mixed evenly, poured into a mold, and cooled to obtain the medicated soap.

9. The application of the herbal extract according to claim 6 in the preparation of personal care products.

10. A method for improving the extraction efficiency of active substances in traditional Chinese medicine formulas, characterized in that, Extraction was performed using a taurine-urea eutectic solvent, and the steps included: The taurine-urea eutectic solvent was mixed with the powder of traditional Chinese medicine components, and the mixture was extracted by ultrasonication, centrifuged, and filtered to obtain the extract of traditional Chinese medicine formula containing active substances. The powdered traditional Chinese medicine components are obtained by mixing and pulverizing Rehmannia glutinosa, Angelica sinensis, Sophora flavescens, Dictamnus dasycarpus, Salvia miltiorrhiza, Glycyrrhiza uralensis, Scutellaria baicalensis, and Smilax glabra; the active substances are total flavonoids and alkaloids. The preparation method of the taurine-urea eutectic solvent is as follows: Taurine and urea were mixed in a molar ratio of 1:(4~6) and heated to obtain a taurine-urea eutectic solvent.

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