Method for induced differentiation of human pluripotent stem cells into functional intestinal organs

By employing a four-stage induction culture medium method and utilizing specific activators and growth factor regulators, the structural heterogeneity problem of existing 3D intestinal organoid culture systems has been solved, enabling the standardized and large-scale production of functional intestinal organoids, which are suitable for drug screening, toxicity evaluation, and regenerative medicine.

CN121825855APending Publication Date: 2026-04-10SHANGHAI NENGSHAN BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202610306918.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-13
Publication Date
2026-04-10

AI Technical Summary

Technical Problem

Existing 3D intestinal organoid culture systems suffer from structural heterogeneity and lack key microenvironment components, making it impossible to fully simulate complex physiological and pathological states in vivo, which limits their standardization and large-scale application.

Method used

A four-stage induction culture method was adopted, including endoderm induction, hindbrain endoderm induction, intestinal expansion and maturation, and intestinal epithelial differentiation and maturation stages. Specific activators, growth factors and signaling pathway regulators were used to induce human pluripotent stem cells to differentiate into functional intestinal organoids.

Benefits of technology

It has formed 3D intestinal organoids with complete structure, cellular diversity and functional activity, which are suitable for drug screening, toxicity evaluation and disease modeling. It has an efficient and reliable in vitro model platform and supports regenerative medicine applications.

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Abstract

The invention provides a method for induced differentiation of human pluripotent stem cells into functional intestinal organoid, and belongs to the field of stem cell technology and organoid engineering. The core of the invention is to provide a set of culture medium formula system with definite components and clear stages, and the intestinal organs can be efficiently guided to differentiate to various functional cell lineages including epithelial cells, neuroendocrine cells and endothelial cells, so that mature organs highly simulating natural intestinal tracts in cell composition are formed. Through a systematized culture medium formula, a standardized operation process and a multi-stage time sequence induction strategy, the next-generation 3D intestinal organ with structural integrity, cell diversity and functional activity at the same time is successfully constructed. The invention provides an efficient, reliable, repeatable and easy-to-expand advanced in-vitro model for intestinal development, disease mechanism, drug screening, toxicity evaluation and regenerative medicine research, and has important scientific research and transformation application value.
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