Experimental device and method for researching influence of mechanical wave energy on plant phenotype

Abstract

The invention provides a research experiment device for influence of mechanical wave energy on plant phenotypes. The research experiment device comprises a cabin body, the environment adjusting part is used for adjusting the environment in the cabin body; the environment detection part is used for detecting the environment in the cabin body; the phenotype acquisition part is used for acquiring phenotype conditions of plants in the cabin body; the sound field generating component is used for emitting sound waves into the cabin body; the sound field detection component is used for detecting sound waves in the cabin body; and the control part is electrically connected with the environment adjusting part, the environment detection part, the phenotype acquisition part, the sound field generation part and the sound field detection part, and is used for receiving and displaying the detection conditions of the environment detection part and the sound field detection part and controlling the working states of the environment adjusting part and the sound field generation part.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological experimental instruments, in particular to a mechanical wave energy affecting plant phenotype research experimental device and method. BACKGROUND

[0002] Phenotype refers to the external appearance of a biological body, such as shape, structure, size, color, etc., which is mainly determined by the environment and genes. Studying plant phenotype is of great significance to plant growth and development. At present, according to different application scenarios, the phenomics research platform can be divided into two categories: greenhouse type and field phenotype platform. The greenhouse type phenomics facility mainly collects and analyzes the phenotype information of plants or crops under controllable environmental conditions, integrates environmental conditions, matches genomics and phenomics data, and promotes the research process of molecular breeding.

[0003] However, the existing greenhouse type phenomics facility mainly studies the response of plants to biological stress or abiotic stress, and lacks corresponding facilities for studying the influence of sound waves on plant phenotype. SUMMARY

[0004] Therefore, it is necessary to provide a mechanical wave energy affecting plant phenotype research experimental device and method, which can effectively solve the above problems.

[0005] A mechanical wave energy affecting plant phenotype research experimental device, comprising: a cabin body;

[0006] An environmental regulation component is arranged in the cabin body for regulating the environment in the cabin body;

[0007] An environmental detection component is arranged in the cabin body for detecting the environment in the cabin body;

[0008] A phenotype collection component is arranged in the cabin body for collecting the plant phenotype in the cabin body;

[0009] A sound field generation component is arranged in the cabin body for emitting sound waves into the cabin body;

[0010] A sound field detection component is arranged in the cabin body for detecting the sound waves in the cabin body, and

[0011] A total controller is electrically connected with the environmental regulation component, the environmental detection component, the phenotype collection component, the sound field generation component and the sound field detection component, for receiving and displaying the detection conditions of the environmental detection component and the sound field detection component, and controlling the working states of the environmental regulation component and the sound field generation component.

[0012] Optionally, the environment adjusting component comprises an LED array, a refrigeration module, a heating module, a humidifier and a drying filter, the LED array, the refrigeration module, the heating module, the humidifier and the drying filter are fixed in the cabin and electrically connected with the general controller. The LED array is used to provide light in the cabin, the refrigeration module is used to reduce the temperature in the cabin, the heating module is used to increase the temperature in the cabin, the humidifier is used to increase the humidity in the cabin, and the drying filter is used to reduce the humidity in the cabin.

[0013] Optionally, the environment detecting component comprises a light intensity sensor, a light spectrum sensor, an environment temperature sensor and an environment humidity sensor, the light intensity sensor, the light spectrum sensor, the environment temperature sensor and the environment humidity sensor are electrically connected with the general controller, the light intensity sensor is used to detect the light intensity in the cabin, the light spectrum sensor is used to detect the light spectrum in the cabin, the environment temperature sensor is used to detect the temperature in the cabin, and the environment humidity sensor is used to detect the humidity in the cabin, the light intensity sensor, the light spectrum sensor, the environment temperature sensor and the environment humidity sensor send the detection results to the general controller.

[0014] Optionally, the phenotype collecting component comprises a camera element and a position angle adjusting assembly, one end of the position angle adjusting assembly is fixed in the cabin, and the other end forms a moving end, the camera element is fixed on the moving end and electrically connected with the general controller, and the position angle adjusting assembly is used to drive the camera element to move in the cabin.

[0015] Optionally, the phenotype collecting component comprises a camera element and a position angle adjusting assembly, one end of the position angle adjusting assembly is fixed in the cabin, and the other end forms a moving end, the camera element is fixed on the moving end, and the position angle adjusting assembly is used to drive the camera element to move in the cabin.

[0016] Optionally, the position angle adjusting assembly comprises a first sliding rail, a first slider, a second sliding rail, a second slider, a lifting assembly and a rotator, the first sliding rail is fixed on the top of the cabin along the horizontal direction, the first slider is in sliding connection with the first sliding rail and can slide along the first sliding rail, the second sliding rail is fixed on the first slider along the horizontal direction, the second slider is in sliding connection with the second sliding rail and can slide along the second sliding rail, the first sliding rail and the second sliding rail are perpendicular to each other, the lifting assembly is fixed on the second slider, the rotator is fixed on the movable end of the lifting assembly, and the camera element is fixed on the movable end of the rotator.

[0017] Optionally, the camera element comprises one or more of an industrial camera, an infrared camera, a thermal imaging camera, and a fluorescence imaging camera.

[0018] Optionally, the sound field generating component comprises a sound field generator and a power amplifier controller, the sound field generator and the power amplifier controller being electrically connected to the general controller, and the sound field generator being electrically connected to the power amplifier controller.

[0019] Optionally, the sound field generating component comprises a plurality of sound field generators and corresponding power amplifier controllers, the sound field generators being fixed at respective positions in the cabin, and the general controller being capable of individually controlling the power amplifier size and the sound field content of any of the sound field generators.

[0020] The application further provides an experimental method for studying the influence of mechanical wave energy on plant phenotypes, based on any of the above-described experimental devices for studying the influence of mechanical wave energy on plant phenotypes, and comprising the following steps:

[0021] S1, system initialization:

[0022] S2, reading the set environment parameters and adjusting the current environment so that the detected environment parameters meet the set environment parameters;

[0023] S3, reading the set sound field parameters and adjusting the sound field so that the detected sound field parameters meet the set sound field parameters;

[0024] S4, performing phenotype sampling.

[0025] Optionally, step S2 comprises:

[0026] S21, reading the set environment parameters, the set environment parameters comprising temperature setting parameters, humidity setting parameters, light setting parameters, and spectrum setting parameters;

[0027] S22, obtaining the current detected environment parameters and comparing them with the set environment parameters, the current detected environment parameters comprising temperature current parameters, humidity current parameters, light current parameters, and spectrum current parameters; if the current detected environment parameters meet the set environment parameters, step S3 is performed, and if the current detected environment parameters do not meet the set environment parameters, step S23 is performed.

[0028] S23, when the temperature current parameter is greater than the temperature setting parameter, driving the refrigeration module to work, when the temperature current parameter is less than the temperature setting parameter, driving the heating module to work, when the humidity current parameter is greater than the humidity setting parameter, driving the drying filter to work, when the humidity current parameter is less than the humidity setting parameter, driving the humidifier to work, when the illumination current parameter is greater than the illumination setting parameter, increasing the LED array power, when the illumination current parameter is less than the illumination setting parameter, reducing the LED array power, when the light spectrum current parameter is greater than the light spectrum setting parameter, increasing the proportion of purple light in the LED array, when the light spectrum current parameter is less than the light spectrum setting parameter, increasing the proportion of red light in the LED array, and performing S22.

[0029] The present application has the following beneficial effects:

[0030] The mechanical wave energy plant phenotype research experimental device provided by the present application comprises an environment adjusting component, an environment detecting component, a phenotype collecting component, a sound field generating component and a sound field detecting component, the environment adjusting component is used to adjust the environment in the cabin, the sound field generating component is used to emit sound waves into the cabin, and the phenotype collecting component is used to collect the phenotype of the plant, so as to realize the research on the influence of the sound waves on the plant phenotype. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort.

[0032] Figure 1 It is a structural schematic diagram of the mechanical wave energy plant phenotype research experimental device in the present application.

[0033] Figure 2 It is a step diagram of the mechanical wave energy plant phenotype research experimental method in the present application.

[0034] 1-cabin, 2-total controller, 3-environment adjusting component, 31-LED array, 32-refrigeration module, 33-heating module, 34-humidifier, 35-drying filter, 4-environment detecting component, 41-illumination intensity sensor, 42-light spectrum sensor, 43-environment temperature sensor, 44-environment humidity sensor, 5-phenotype collecting component, 51-industrial camera, 52-infrared camera, 53-thermal imaging camera, 54-fluorescence imaging camera, 6-sound field generating component, 61-sound field generator, 62-power amplifier controller, 7-sound field detecting component, 71-microphone. DETAILED DESCRIPTION

[0035] Preferred embodiments of the present application will be described in detail below with reference to the drawings, in which the figures constitute a part of the present application and illustrate embodiments of the present application together with the principles of the present application, but are not intended to limit the scope of the present application.

[0036] As shown in Figure 1 Embodiments of the present application provide a research experimental device for studying the influence of mechanical wave energy on plant phenotypes, which comprises:

[0037] The cabin 1, the general controller 2, the environment adjusting component 3, the environment detecting component 4, the phenotype collecting component 5, the sound field generating component 6 and the sound field detecting component 7 are arranged in the cabin 1 and electrically connected with the general controller 2. The environment adjusting component 3 is used for adjusting the environment in the cabin 1. The environment detecting component 4 is used for detecting the environment in the cabin 1. The phenotype collecting component 5 is used for collecting the phenotype condition in the cabin 1. The sound field generating component 6 is used for emitting sound waves into the cabin 1. The sound field detecting component 7 is used for detecting the sound waves in the cabin 1. The general controller 2 is used for receiving and displaying the detection conditions of the environment detecting component 4 and the sound field detecting component 7, and controlling the working states of the environment adjusting component 3 and the sound field generating component 6.

[0038] The research experimental device for studying the influence of mechanical wave energy on plant phenotypes comprises the environment adjusting component 3, the environment detecting component 4, the phenotype collecting component 5, the sound field generating component 6 and the sound field detecting component 7. The environment adjusting component 3 is used for adjusting the environment in the cabin 1. The sound field generating component 6 is used for emitting sound waves into the cabin 1. The phenotype collecting component 5 is used for collecting the phenotype condition of the plant, so as to realize the research on the influence of sound waves on plant phenotypes.

[0039] Specifically, the environment adjusting component 3 comprises an LED array 31, a refrigeration module 32, a heating module 33, a humidifier 34 and a drying filter 35, which are fixed in the cabin 1 and electrically connected with the general controller 2. The LED array 31 is used for providing light to the cabin 1. The refrigeration module 32 is used for reducing the temperature in the cabin 1. The heating module 33 is used for increasing the temperature in the cabin 1. The humidifier 34 is used for increasing the humidity in the cabin 1. The drying filter 35 is used for reducing the humidity in the cabin 1.

[0040] Specifically, the environment detection component 4 includes a light intensity sensor 41, a light spectrum sensor 42, an environment temperature sensor 43, and an environment humidity sensor 44, which are electrically connected to the general controller 2. The light intensity sensor 41 is used to detect the light intensity in the cabin 1, the light spectrum sensor 42 is used to detect the light spectrum in the cabin 1, the environment temperature sensor 43 is used to detect the temperature in the cabin 1, and the environment humidity sensor 44 is used to detect the humidity in the cabin 1. The light intensity sensor 41, the light spectrum sensor 42, the environment temperature sensor 43, and the environment humidity sensor 44 send the detection results to the general controller 2.

[0041] Specifically, the phenotype collection component 5 includes a camera element and a position and angle adjusting assembly. One end of the position and angle adjusting assembly is fixed in the cabin 1, and the other end forms a moving end. The camera element is fixed on the moving end and electrically connected to the general controller 2. The position and angle adjusting assembly is used to drive the camera element to move in the cabin 1. The position and angle adjusting assembly can drive the camera element to move in the cabin 1, which can avoid obstruction and make the camera element shoot more clearly and accurately. On the other hand, the position and angle adjusting assembly can adjust the position of the camera element and shoot specific parts of the plant.

[0042] Further, the position and angle adjusting assembly includes a first sliding rail, a first slider, a second sliding rail, a second slider, a lifting assembly, and a rotator. The first sliding rail is fixed on the top of the cabin 1 in the horizontal direction. The first slider is in sliding connection with the first sliding rail and can slide along the first sliding rail. The second sliding rail is fixed on the first slider in the horizontal direction. The second slider is in sliding connection with the second sliding rail and can slide along the second sliding rail. The first sliding rail and the second sliding rail are perpendicular to each other. The lifting assembly is fixed on the second slider. The rotator is fixed on the movable end of the lifting assembly. The camera element is fixed on the movable end of the rotator. The first slider and the second slider cooperate to drive the camera element to move in the horizontal plane. The lifting assembly is used to drive the camera element to lift in the vertical direction. The rotator is used to drive the camera element to rotate in the vertical direction, so that the camera element can shoot the plant without dead angle in the cabin 1.

[0043] Further, the camera element includes one or more of an industrial camera 51, an infrared camera 52, a thermal imaging camera 53, and a fluorescence imaging camera 54.

[0044] Specifically, the sound field generating component 6 includes a sound field generator 61 and a power amplifier controller 62. The sound field generator 61 and the power amplifier controller 62 are electrically connected to the main controller 2. The power amplifier controller 62 receives control signals from the main controller 2 to adjust the sound output of the sound field generator 61, and the sound field generator 61 receives control signals from the main controller 2 to emit preset sound waves.

[0045] Furthermore, the sound field generating component 6 includes multiple sound field generators 61 and corresponding power amplifier controllers 62. The sound field generators 61 are fixed at various positions within the cabin 1. The main controller 2 can individually control the power amplification and sound field content of any sound field generator 61, so that it forms a composite sound wave simulating a specific scene within the cabin 1.

[0046] Specifically, the sound field detection component 7 includes a microphone 71, which is fixed at various positions inside the cabin 1 to detect the magnitude of the sound waves at its location.

[0047] like Figure 2 As shown, the present invention also provides an experimental method for studying the influence of mechanical wave energy on plant phenotypes, which includes the following steps:

[0048] S1. System Initialization:

[0049] S2. Read the set environment parameters and adjust the current environment to make the detection environment parameters meet the set environment parameters;

[0050] S3. Read the set sound field parameters and adjust the sound field so that the detected sound field parameters meet the set sound field parameters;

[0051] S4. Perform phenotypic sampling.

[0052] Furthermore, step S2 includes:

[0053] S21. Read the set environmental parameters, which include temperature setting parameters, humidity setting parameters, light setting parameters, and spectrum setting parameters;

[0054] S22. Obtain the current detection environment parameters and compare them with the set environment parameters. The current detection environment parameters include the current temperature, current humidity, current illumination, and current spectrum. If the current detection environment parameters meet the set environment parameters, proceed to step S3. If the current detection environment parameters do not meet the set environment parameters, proceed to step S23.

[0055] S23. When the current temperature parameter is greater than the set temperature parameter, the cooling module is driven to work; when the current temperature parameter is less than the set temperature parameter, the heating module is driven to work; when the current humidity parameter is greater than the set humidity parameter, the dryer filter is driven to work; when the current humidity parameter is less than the set humidity parameter, the humidifier is driven to work; when the current light parameter is greater than the set light parameter, the LED array power is increased; when the current light parameter is less than the set light parameter, the LED array power is decreased; when the current spectrum parameter is greater than the set spectrum parameter, the proportion of violet light in the LED array is increased; when the current spectrum parameter is less than the set spectrum parameter, the proportion of red light in the LED array is increased; and S22 is executed.

[0056] Furthermore, step S3 includes:

[0057] S31. Read the set sound wave parameters. The set sound wave parameters include sound wave amplitude setting parameters and sound wave frequency setting parameters.

[0058] S32. Obtain the current detected sound wave parameters and compare them with the set sound wave parameters. The current detected sound wave parameters include the current sound wave amplitude parameter and the current sound wave frequency parameter. If the current detected sound wave parameters match the set sound wave parameters, proceed to step S4. If the current detected sound wave parameters do not match the set sound wave parameters, proceed to step S33.

[0059] S33. When the current parameter of the sound wave amplitude is greater than the set parameter of the sound wave amplitude, drive the power amplifier controller to reduce the sound wave amplitude. When the current parameter of the sound wave amplitude is less than the set parameter of the sound wave amplitude, drive the power amplifier controller to increase the sound wave amplitude. When the current parameter of the sound wave frequency is greater than the set parameter of the sound wave frequency, drive the power amplifier controller to reduce the sound wave frequency. When the current parameter of the sound wave frequency is less than the set parameter of the sound wave frequency, drive the power amplifier controller to increase the sound wave frequency, and execute S32.

[0060] The beneficial effects of this invention are:

[0061] The experimental apparatus for studying the influence of mechanical wave energy on plant phenotype proposed in this invention includes an environmental regulation component, an environmental detection component, a phenotype acquisition component, a sound field generating component, and a sound field detection component. The environmental regulation component adjusts the environment inside the chamber, the sound field generating component emits sound waves into the chamber, and the phenotype acquisition component collects the phenotype of the plants to achieve the study of the influence of sound waves on plant phenotype.

[0062] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A research apparatus for investigating the effect of mechanical wave energy on plant phenotypes, characterized by, The application relates to a plant phenotype acquisition device. The device comprises a cabin, an environment adjusting component arranged in the cabin for adjusting the environment in the cabin, an environment detecting component arranged in the cabin for detecting the environment in the cabin, a phenotype acquisition component arranged in the cabin for acquiring the plant phenotype in the cabin, a sound field generating component arranged in the cabin for emitting sound waves into the cabin, a sound field detecting component arranged in the cabin for detecting the sound waves in the cabin, and a general controller electrically connected with the environment adjusting component, the environment detecting component, the phenotype acquisition component, the sound field generating component and the sound field detecting component, for receiving and displaying the detection results of the environment detecting component and the sound field detecting component, and controlling the working states of the environment adjusting component and the sound field generating component. The environment adjusting component comprises an LED array, a refrigeration module, a heating module, a humidifier and a drying filter, the LED array, the refrigeration module, the heating module, the humidifier and the drying filter are fixed in the cabin and electrically connected with the general controller, the LED array is used for providing light to the cabin, the refrigeration module is used for reducing the temperature in the cabin, the heating module is used for increasing the temperature in the cabin, the humidifier is used for increasing the humidity in the cabin, and the drying filter is used for reducing the humidity in the cabin. The environment detecting component comprises a light intensity sensor, a light spectrum sensor, an environment temperature sensor and an environment humidity sensor, the light intensity sensor, the light spectrum sensor, the environment temperature sensor and the environment humidity sensor are electrically connected with the general controller, the light intensity sensor is used for detecting the light intensity in the cabin, the light spectrum sensor is used for detecting the light spectrum in the cabin, the environment temperature sensor is used for detecting the temperature in the cabin, and the environment humidity sensor is used for detecting the humidity in the cabin, and the light intensity sensor, the light spectrum sensor, the environment temperature sensor and the environment humidity sensor send the detection results to the general controller. The phenotype acquisition component comprises a camera element and a position angle adjusting assembly, one end of the position angle adjusting assembly is fixed in the cabin, the other end forms a moving end, the camera element is fixed on the moving end and electrically connected with the general controller, and the position angle adjusting assembly is used for driving the camera element to move in the cabin. ​ ​ ​ ​ 2. The apparatus of claim 1, wherein the mechanical wave energy affects the plant phenotype. ​ 3. The apparatus of claim 2, wherein the mechanical wave energy is ultrasonic energy. ​ 4. The apparatus of claim 1, wherein the mechanical wave energy affects the plant phenotype of the plant. ​ 5. The apparatus of claim 4, wherein the mechanical wave energy is ultrasonic energy. The position angle adjusting assembly comprises a first sliding rail, a first slider, a second sliding rail, a second slider, a lifting assembly and a rotator, the first sliding rail is fixed on the top of the cabin body in a horizontal direction, the first slider is in sliding connection with the first sliding rail and can slide along the first sliding rail, the second sliding rail is fixed on the first slider in a horizontal direction, the second slider is in sliding connection with the second sliding rail and can slide along the second sliding rail, the first sliding rail and the second sliding rail are perpendicular to each other, the lifting assembly is fixed on the second slider, the rotator is fixed on the movable end of the lifting assembly, and the camera element is fixed on the movable end of the rotator.

6. The apparatus of claim 5, wherein the mechanical wave energy is ultrasonic energy. The camera element comprises one or more of an industrial camera, an infrared camera, a thermal imaging camera and a fluorescence imaging camera.

7. The apparatus of claim 1, wherein the mechanical wave energy affects the plant phenotype of the research experiment. The sound field generating component comprises a sound field generator and a power amplifier controller, the sound field generator and the power amplifier controller are electrically connected with the total controller, and the sound field generator is electrically connected with the power amplifier controller.

8. The apparatus of claim 7, wherein the mechanical wave energy is ultrasonic energy. The sound field generating component comprises a plurality of sound field generators and corresponding power amplifier controllers, the sound field generators are fixed at different positions in the cabin body, and the total controller can individually control the power amplifier size and sound field content of any sound field generator.

9. An experimental method for studying the effect of mechanical wave energy on plant phenotypes, characterized by, The research experimental device for the influence of mechanical wave energy on plant phenotype based on any one of claims 1-8 is realized, comprising the following steps: S1, system initialization; S2, reading the set environment parameters and adjusting the current environment to make the detected environment parameters meet the set environment parameters; S3, reading the set sound field parameters and adjusting the sound field to make the detected sound field parameters meet the set sound field parameters; S4, performing phenotype sampling.

10. The experimental method of claim 9, wherein the mechanical wave energy affects the plant phenotype. Step S2 comprises: S21, reading the set environment parameters, the set environment parameters comprising temperature setting parameters, humidity setting parameters, illumination setting parameters and spectrum setting parameters; S22, obtaining the current detected environment parameters and comparing them with the set environment parameters, the current detected environment parameters comprising temperature current parameters, humidity current parameters, illumination current parameters and spectrum current parameters; if the current detected environment parameters meet the set environment parameters, step S3 is performed, otherwise, step S23 is performed; S23, when the temperature current parameters are greater than the temperature setting parameters, driving the refrigeration module to work, when the temperature current parameters are less than the temperature setting parameters, driving the heating module to work, when the humidity current parameters are greater than the humidity setting parameters, driving the drying filter to work, when the humidity current parameters are less than the humidity setting parameters, driving the humidifier to work, when the illumination current parameters are greater than the illumination setting parameters, increasing the power of the LED array, when the illumination current parameters are less than the illumination setting parameters, reducing the power of the LED array, when the spectrum current parameters are greater than the spectrum setting parameters, increasing the proportion of purple light in the LED array, and when the spectrum current parameters are less than the spectrum setting parameters, increasing the proportion of red light in the LED array; and performing S22.

Images