Organic selenium-rich bean sprout culture medium as well as preparation method and application thereof
By preparing a cultivation substrate by mixing agar with organic selenium compounds, the operational difficulties of home-grown selenium-rich bean sprouts have been solved, achieving efficient and targeted utilization of selenium and simplifying the operation, thus improving the edible rate of selenium.
Patent Information
- Application Number
- CN202610066757.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-19
- Publication Date
- 2026-04-14
AI Technical Summary
In the existing technology, there is a lack of effective and easy-to-operate methods for home production of selenium-rich bean sprouts. Hydroponics requires frequent addition of selenium solution, and intelligent sprouting machines are expensive and the selenium is mainly distributed in the roots and cannot be eaten, resulting in low selenium utilization.
A cultivation substrate was prepared by mixing agar with an organic selenium compound. Selenomethionine was evenly distributed in the agar gel. Legume seeds were cultivated on the cultivation substrate, simplifying the operation and enabling targeted utilization of selenium.
This approach enables the efficient and targeted utilization of selenium, reduces labor intensity and equipment costs, increases the selenium consumption rate, and meets the needs of household production.
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Figure CN121844920A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of agricultural production technology, and in particular to a selenium-rich soybean sprout cultivation substrate, its preparation method, and its application. Background Technology
[0002] Organic selenium is a form of selenium, an essential trace element for the human body. Compared to inorganic selenium, it has stronger antioxidant capabilities and functions such as anti-cancer and anti-inflammatory effects, but a dose-response effect also exists. The absorption rate of organic selenium in the human body is higher than that of inorganic selenium, and more can be recovered and utilized from organic selenium. Using food as a source of selenium is considered a safe and effective way to supplement the body's selenium intake. Legumes have a strong ability to absorb and transport selenium, among which soybean sprouts, mung bean sprouts, and pea sprouts are highly nutritious and have a short production cycle. As consumers' recognition of the nutritional value and health benefits of sprouts continues to increase, the bean sprout industry is expanding. Organic selenium-rich bean sprouts have given bean sprouts new health benefits. Currently, the common methods for sun-drying mung bean sprouts are hydroponics or using intelligent germination machines. However, hydroponics requires frequent manual addition of selenium solution, while using intelligent germination machines results in selenium being mainly distributed in the roots of the sprouts and not being ingested. Furthermore, intelligent germination machines are expensive and not suitable for home use. Therefore, a core technological bottleneck in the current home production of selenium-enriched mung bean sprouts is the lack of an effective and easy-to-operate method for enriching the sprouts with organic selenium. Summary of the Invention
[0003] The present invention aims to provide a selenium-rich organic bean sprout cultivation substrate, its preparation method and application, in order to solve the current problems of selenium enrichment being troublesome and ineffective.
[0004] To achieve the above objectives, the present invention provides the following technical solution:
[0005] A selenium-rich bean sprout cultivation substrate includes a cultivation substrate, which is formed by mixing and solidifying agar and an organic selenium compound, with the organic selenium compound evenly distributed in the gel grid formed by the agar.
[0006] Furthermore, the organoselenium compound is selenomethionine.
[0007] Furthermore, in the cultivation substrate, the concentration of organic selenium compounds, calculated as selenium, is 4-6 mg / L, and the concentration of agar in the cultivation substrate preparation solution is 11-13 g / L.
[0008] A method for preparing a selenium-rich soybean sprout cultivation substrate includes the following steps:
[0009] S1: Mix agar with water, heat and stir until the agar is completely dissolved to obtain an agar solution;
[0010] S2: After the agar solution cools to 45-60℃, add the organic selenium compound to the agar solution and stir evenly to obtain the cultivation substrate preparation solution;
[0011] S3: Pour the cultivation substrate preparation solution into the cultivation container and wait for it to cool and solidify to obtain the cultivation substrate.
[0012] A method for cultivating selenium-rich bean sprouts, using a cultivation substrate to cultivate legume seeds, includes the following steps:
[0013] A1: Soak legume seeds in hot water to remove floating seeds, stir for 2-3 minutes, and remove the remaining seeds and let them dry until they no longer drip water;
[0014] A2: Simply scatter the seeds evenly into the cultivation container, ensuring they are evenly distributed on the cultivation substrate.
[0015] Furthermore, legume seeds can be mung beans, soybeans, or peas, and the cultivation conditions are: temperature 20-30℃, and a dark environment.
[0016] Furthermore, the planting density for legume seeds is 9-10g of dry seeds per square decimeter of cultivation substrate area.
[0017] The principles and beneficial effects of the technical solution are as follows:
[0018] This invention provides a selenium-rich organic bean sprout cultivation substrate, its preparation method, and its applications.
[0019] 1. Selecting selenomethionine as an organic selenium source can better match the selenium enrichment of bean sprouts. At the same time, selenomethionine has high bioavailability and is easily absorbed and utilized by the human body.
[0020] 2. When combined with agar as a selenium carrier and growth carrier, it is simpler to operate compared to hydroponics and intelligent germination machines. It does not require frequent addition of selenium solution. At the same time, it does not require electric drive and complex circulation system, which reduces labor intensity, technical threshold and equipment cost.
[0021] 3. In a solid cultivation substrate, selenium is confined to the area accessible to the roots, enabling efficient and targeted utilization of selenium. Attached Figure Description
[0022] Figure 1 This is a comparative experimental diagram of Example 1 in this invention;
[0023] Figure 2 This is a comparative experimental diagram of Example 2 in this invention;
[0024] Figure 3 This is a control diagram of the experiment in Example 3 of the present invention;
[0025] Figure 4 This is a control diagram of the experiment in Example 4 of this invention;
[0026] Figure 5 This is a control diagram of the experiment in Example 5 of the present invention;
[0027] Figure 6 This is a graph showing the selenium content in Example 5 of this invention. Detailed Implementation
[0028] The present invention will now be described in further detail with reference to the accompanying drawings, implementation methods, and comparative test data:
[0029] Example 1
[0030] Screening of organic selenium forms:
[0031] Experimental seeds: Eighteen Arhat mung bean seeds, selected with plump grains of uniform size, and divided into several portions.
[0032] Selenium source settings:
[0033] Treatment group A: 10 mg / L selenium, sourced from selenomethionine
[0034] Treatment group B: 10 mg / L selenium, derived from selenomethylcysteine.
[0035] Control group (CK): No selenium source added.
[0036] Cultivation method: The bean sprouts are cultivated using a fully automatic intelligent bean sprout machine, with various selenium source solutions used as cultivation water, and the cultivation is carried out automatically following the standard procedures of the bean sprout machine.
[0037] Measurement indicators: The growth of bean sprouts in each group was measured to examine the inhibitory effect of selenium source on the growth of bean sprouts.
[0038] Experimental results: The growth parameters of bean sprouts in each group are shown in Table 1.
[0039]
[0040] Table 1
[0041] Conclusion: Based on Table 1 and appendix Figure 1 Analysis shows that Se-L-methylcysteine severely inhibits the growth of bean sprouts and is not suitable as an organic selenium source. While selenomethionine inhibits the growth of mung bean sprouts to some extent, it also promotes the growth of fresh weight and dry weight after root removal. Therefore, selenomethionine is the optimal choice as an organic selenium source.
[0042] Example 2
[0043] Screening of selenium application methods:
[0044] Selenium source: selenomethionine screened in Example 1 was used uniformly, with a selenium concentration of 10 mg / L.
[0045] Method settings:
[0046] Intelligent Germination Machine M1: Same as Example 1, using selenium solution as the cultivation water.
[0047] Traditional hydroponics M2: Under the same environmental conditions, use selenium-containing nutrient solution for static hydroponics, and replace the same amount of nutrient solution daily.
[0048] Cultivation substrate M3: Prepare a 7g / L agar solution, add selenomethionine solution to make the selenium concentration 10mg / l, pour into a culture container and cool to form a solid cultivation substrate for bean sprout cultivation.
[0049] Experimental Results and Analysis:
[0050] refer to Figure 2 All selenium application methods ensured the normal growth of bean sprouts. Based on the specific growth conditions and operational procedures, it was found that:
[0051] Intelligent bean sprout machine: Edible selenium content (above-ground parts) is 290 mg / kg (DW) (15 mg / kg FW); Total selenium content (above-ground parts + underground parts) is 459 mg / kg. Disadvantages: Roots are the main distribution area of selenium, resulting in excessively long root systems; excessive waste of selenium solution.
[0052] Hydroponics: Hydroponics can effectively control root growth and achieve a suitable root-to-shoot ratio. Disadvantages: It requires frequent addition of selenium solution, necessitating significant manual intervention.
[0053] Cultivation substrate: Simple to operate, no need to frequently add selenium solution; saves electricity and nutrient solution compared to smart bean sprout machines.
[0054] Conclusion: Using cultivation substrate for selenium application is the simplest method and has the highest selenium source utilization rate, making it the optimal choice for selenium application.
[0055] Example 3
[0056] Organic selenium concentration screening:
[0057] Cultivation method: The M1 method (intelligent germination machine cultivation) in Example 2 was adopted, selenomethionine was selected as the organic selenium source, and selenium solution was used as the cultivation water.
[0058] Concentration gradient settings:
[0059] a: Organic selenium concentration is 10 mg / L
[0060] b: Organic selenium concentration is 5 mg / L
[0061] c: Organic selenium concentration is 2.5 mg / L
[0062] d: Organic selenium concentration is 1.25 mg / L
[0063] e: Organic selenium concentration is 0 mg / L (control)
[0064] Measurement indicators: Selenium content and growth parameters of bean sprouts in each group were measured.
[0065] Experimental results:
[0066] The comparison results of selenium concentration test data are shown in Table 2, and the comparison results of growth parameters are shown in Table 3.
[0067]
[0068] Table 2
[0069]
[0070] Table 3
[0071] Analysis and Conclusion:
[0072] Refer to Tables 2 and 3 and Figure 3 It is known that bean sprouts treated with 10 mg / L selenium have the highest selenium content, but their growth is most severely inhibited. According to the Chinese Dietary Reference Intakes (2023 edition), the average human requirement for selenium is 0.05 mg / day (FW), the reference intake is 0.06 mg / day (FW), and the tolerable upper intake level is 0.4 mg / day (FW). Consuming bean sprouts treated with 10 mg / L selenium is too high a concentration. Only 20-26.7g is needed to reach the tolerable upper intake level. The amount consumed is too small and does not reflect actual consumption conditions, making it very easy to exceed the limit.
[0073] Further screening of selenium concentrations revealed that a concentration that neither inhibited growth nor compromised daily nutritional needs was selected. Ultimately, a 5 mg / L selenium treatment was chosen, resulting in a selenium content of 23.7 mg / kg DW (1.9 mg / kg FW) in the edible portion of the bean sprouts. This means that only 31.58g of bean sprouts are needed to meet the average selenium requirement, which is easily achieved. However, exceeding the tolerable upper intake level requires 210.52g. The normal adult intake of bean sprouts per meal is 100-150g, falling within the safe limit and unlikely to be exceeded. Furthermore, compared to bean sprouts treated with 2.5 mg / L selenium, the growth was similar, but the selenium content of the 5 mg / L treatment was 2.41 times higher. Therefore, the final selenium concentration was determined to be 5 mg / L.
[0074] Example 4
[0075] Screening for cultivation density:
[0076] Preparation of cultivation substrate: According to method M3 in Example 2, an agar cultivation substrate containing selenomethionine (SeMet, selenium concentration 5 mg / L) (agar concentration 7 g / L) was prepared.
[0077] Culture container: Black food-grade round box, with a bottom diameter of 11.5 cm (area of about 104 cm²) and a top diameter of 17.5 cm (area of about 240 cm²), a height of 11.5 cm, and a volume of about 1750 mL. Each box is filled with 165 mL of the above-mentioned cultivation substrate, and the substrate thickness is uniform after solidification.
[0078] Seeding weight gradient settings:
[0079] D1: 10g dried mung bean seeds / box
[0080] D2: 15g dried mung bean seeds / box
[0081] D3: 20g dried mung bean seeds / box
[0082] Cultivation conditions: All density groups were cultivated in the same environment (temperature 22±1℃, humidity 80±5%, darkness) for 5 days.
[0083] Experimental results data:
[0084] The yield and rearing density data for each group are shown in Table 4.
[0085]
[0086] Table 4
[0087] Cultivation density is the ratio of the number of seeds sown (in grams) to the area of the cultivation substrate at the bottom of the cultivation container.
[0088] Analysis and Conclusion:
[0089] refer to Figure 4 As shown in Table 4, 20g seeds / box (D3) rotted early due to overcrowding and fungal decay, making normal cultivation impossible; the sowing-to-yield ratio of 15g seeds / box (D2) was 1:7.06; and the sowing-to-yield ratio of 10g seeds / box (D1) was 1:9. It is evident that the growth of bean sprouts in the D2 gradient was significantly inhibited. Therefore, 10g seeds / box (D1), i.e., a cultivation density of 9.6g / dm², was ultimately determined to be the optimal choice.
[0090] Example 5
[0091] Selection of agar concentration:
[0092] Experimental seeds: 13.8g of Eighteen Arhats mung bean seeds
[0093] Cultivation container: a black plastic box measuring 13.5cm × 11cm × 14.7cm.
[0094] Setting the agar concentration gradient in the cultivation substrate:
[0095] G1: Agar concentration 9g / L, organic selenium concentration 5mg / L, total cultivation substrate of 227ml.
[0096] G2: Agar concentration 10g / L, organic selenium concentration 5mg / L, total cultivation substrate of 227ml.
[0097] G3: Agar concentration 11g / L, organic selenium concentration 5mg / L, total volume of 227ml cultivation substrate.
[0098] G4: Agar concentration 12g / L, organic selenium concentration 5mg / L, total volume of 227ml cultivation substrate.
[0099] G6: Agar concentration 13g / L, organic selenium concentration 5mg / L, total volume of 227ml cultivation substrate.
[0100] G7: Agar concentration 14g / L, organic selenium concentration 5mg / L, total volume of 227ml cultivation substrate.
[0101] Preparation of cultivation substrate: Weigh out agar powder according to the concentration, dissolve it in the corresponding volume of hot water, add the calculated selenomethionine stock solution, mix well and pour into a basin to solidify.
[0102] Culture conditions: All groups were cultured in the same environment.
[0103] Experimental results data:
[0104] The growth parameters of each group of bean sprouts are shown in Table 5.
[0105]
[0106] Table 5
[0107] Analysis and Conclusion:
[0108] Refer to Table 5. Figure 5-6 It can be seen that as the concentration of agar increases, the selenium content of the edible portion shows a decreasing trend, especially at 9-11 g / L, where the decreasing trend is most obvious. The dry weight selenium content decreases from 54.2 mg / kg to 38.7 mg / kg, and the fresh weight selenium content decreases from 4.18 μg / g to 3.18 μg / g. The decrease slows down in the later stages.
[0109] Based on growth parameters and selenium content, mung bean sprouts show the best results when treated with agar at a concentration of 12 g / L, making it the optimal choice. The fresh weight selenium content is 3.10 μg / g. A single serving yields approximately 99g, with a total selenium content of 217 μg. Consuming this once provides the benefits of selenium supplementation (60 μg / day) and general health maintenance (100-200 μg / day).
[0110] Example 6
[0111] Based on the optimization results of the above experiments, a complete cultivation program was implemented.
[0112] Preparation of cultivation substrate:
[0113] Weigh out agar powder, add it to deionized water, heat and stir until well mixed to obtain an agar solution with an agar concentration of 12 g / L;
[0114] Once the agar solution has cooled to 45-6℃, add selenomethionine (SeMet), stir and mix well to make the final concentration of selenium in the mixture 5 mg / L, thus obtaining the cultivation substrate preparation solution;
[0115] Pour an appropriate amount of the cultivation substrate preparation solution into the cultivation container, and after cooling, a gel-like cultivation substrate will be obtained.
[0116] Seed treatment:
[0117] Soak legume seeds (such as mung beans, soybeans, or peas) in hot water to remove any floating seeds. Stir for 2-3 minutes, then remove the remaining seeds and let them dry until they no longer drip water.
[0118] Simply sow the legume seeds evenly into the cultivation container at a cultivation density of 9.6 g / dm², ensuring they are evenly distributed on the cultivation substrate.
[0119] Cultivation and management: Simply place in a dark environment with a temperature of 20-30℃, without the need to replace the substrate or nutrient solution; harvesting can be done in 5-7 days.
[0120] The above descriptions are merely embodiments of the present invention, and common knowledge regarding specific technical solutions or characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.
Claims
1. A selenium-rich substrate for cultivating bean sprouts, characterized in that: The product includes a cultivation substrate, which is formed by mixing and solidifying agar and an organoselenium compound, wherein the organoselenium compound is uniformly distributed in the gel grid formed by the agar.
2. The organic selenium-rich bean sprout cultivation substrate according to claim 1, characterized in that: The organoselenium compound is selenomethionine.
3. The organic selenium-rich bean sprout cultivation substrate according to claim 2, characterized in that: In the cultivation substrate, the concentration of the organic selenium compound, calculated as selenium, is 4-6 mg / L, and the concentration of agar in the cultivation substrate preparation solution is 11-13 g / L.
4. A method for preparing a selenium-rich organic bean sprout cultivation substrate according to any one of claims 1-3, characterized in that: Includes the following steps: S1: Mix agar with water, heat and stir until the agar is completely dissolved to obtain an agar solution; S2: After the agar solution cools to 45-60℃, add an organic selenium compound to the agar solution and stir evenly to obtain the cultivation substrate preparation solution; S3: Pour the prepared cultivation substrate solution into a cultivation container and allow it to cool and solidify to obtain the cultivation substrate.
5. A method for cultivating selenium-rich bean sprouts, characterized in that: The application of the cultivation substrate described in any one of claims 1-3 for the cultivation of leguminous seeds includes the following steps: A1: Soak legume seeds in hot water to remove floating seeds, stir for 2-3 minutes, and remove the remaining seeds and let them dry until they no longer drip water; A2: Simply scatter the seeds evenly into the cultivation container, ensuring they are evenly distributed on the cultivation substrate.
6. The method for cultivating selenium-rich bean sprouts according to claim 5, characterized in that: The cultivation conditions are: temperature 20-30℃, and dark environment.
7. The method for cultivating selenium-rich bean sprouts according to claim 6, characterized in that: The planting density for legume seeds is 9-10g of dry seeds per square decimeter of cultivation substrate area.