Clostridium butyricum microecological preparation composition for improving sperm quality of livestock and poultry

Abstract

The invention relates to the field of microorganisms, and particularly discloses a clostridium butyricum microecological preparation composition for improving the sperm quality of livestock and poultry. The preparation method comprises the following steps: selecting materials such as cortex phellodendri to prepare mixed powder, using materials such as zinc-rich edible yeast powder to prepare a pasty bacterial colony proliferation culture medium, using materials such as the mixed powder and clostridium butyricum dry powder to prepare activated clostridium butyricum bacterial sludge, and performing mixed fermentation on the activated clostridium butyricum bacterial sludge and the pasty bacterial colony proliferation culture medium; and finally, pouring a mixed solution formed by xanthan gum, fructo-oligosaccharide and purified water into mixed dry powder of materials such as the bacteria-rich fermentation mixture, L-carnitine and the like to obtain a blend wet material, and drying the blend wet material to obtain the microecological preparation composition. The microecological preparation composition is added into the feed, so that the palatability and the nutrient utilization degree of the feed can be effectively improved, the ingestion and digestion of livestock and poultry are promoted, the organism immunity of the livestock and poultry is enhanced, and the semen quality of the livestock and poultry is improved on the premise of avoiding overdraft of the livestock and poultry.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to a Clostridium butyricum microecological preparation composition for improving the quality of livestock and poultry sperm. Background Technology

[0002] With the rapid development of modern livestock and poultry breeding technology, the traditional livestock and poultry breeding model provides a growth and reproduction environment for livestock and poultry, relying on their natural growth and development. However, in modern society, the demand for food has increased significantly, and the traditional natural breeding model cannot meet market demand. Therefore, large-scale breeding farms have emerged, and artificial insemination technology is used to improve the fertilization rate of livestock and poultry eggs, thereby increasing the reproductive efficiency of livestock and poultry. Although artificial insemination technology can reduce the risk of livestock and poultry reproduction to a certain extent, it has very high requirements for semen quality. The semen used for insemination is taken from breeding livestock and poultry, and the quality of breeding livestock and poultry semen is affected by factors such as the breeding environment and daily diet. In particular, the daily diet of breeding livestock and poultry has a significant impact on semen quality. In the breeding process, in order to avoid the decline in vitality of breeding livestock and poultry due to disease, antibiotics are generally added to the diet of breeding livestock and poultry. However, long-term consumption of diet containing antibiotics will lead to the emergence of many drug-resistant bacteria, which is not conducive to maintaining the health of breeding livestock and poultry in the long term.

[0003] Chinese patent CN105456654B discloses a pharmaceutical composition and its preparation method for improving boar semen quality. The composition comprises dandelion, Fraxinus rhynchophylla bark, Phellodendron bark, Astragalus complanatus seed, Anemarrhena asphodeloides rhizome, Cuscuta chinensis seed, Epimedium, Dipsacus asper, Achyranthes bidentata root, Rehmannia glutinosa root (raw and processed), Dioscorea hypoglauca root, Poria cocos, Angelica sinensis root, Rubus idaeus fruit, Lycium barbarum fruit, and Morus alba fruit. These ingredients are extracted and then combined with yangqi stone and excipients. This invention can significantly improve boar libido and semen quality, and is naturally free of drug residues and drug resistance.

[0004] During the application process, the applicant discovered that the above-mentioned application has defects in its use. Although the drug composition disclosed in the application improves the quality of livestock semen to a certain extent, adding this drug to feed will affect the palatability of the feed, reduce the amount of food consumed by livestock, and make it impossible to maintain the long-term good condition of livestock. Moreover, the principle of this drug is mainly to stimulate the sexual desire of livestock and improve sperm activity, rather than to improve sperm quality by improving the overall physiological conditions of livestock. Long-term use will cause overdraft of breeding livestock. Summary of the Invention

[0005] The purpose of this invention is to provide a Clostridium butyricum microecological preparation composition for improving the quality of livestock and poultry sperm, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A Clostridium butyricum microecological preparation composition for improving sperm quality in livestock and poultry, comprising the following raw materials in parts by weight: 10-12 parts Phellodendron bark, 5-10 parts Anemarrhena rhizome, 1-3 parts Epimedium, 10-15 parts Achyranthes root, 5-10 parts Rehmannia root, 5-10 parts Angelica sinensis root, 1-5 parts Lycium barbarum fruit, 200-250 parts L-carnitine, 3-6 parts Vitamin E powder, 1-3 parts folic acid, and 10-12 parts taurine. Oyster powder 30-50 parts, Cordyceps militaris powder 150-200 parts, soy protein isolate 1500-2000 parts, whey protein concentrate 4500-5000 parts, zinc-enriched edible yeast powder 30-50 parts, sorbitol 2000-2500 parts, xanthan gum 50-70 parts, fructooligosaccharides 30-50 parts, purified water 2000-3000 parts, Clostridium butyricum dry powder 100-200 parts.

[0008] Preferably, the method for preparing the Clostridium butyricum microecological preparation composition includes the following steps:

[0009] S1. Weigh each group of raw materials according to the weight ratio and set aside;

[0010] S2. Mix Phellodendron bark, Anemarrhena rhizome, Epimedium, Achyranthes root, Rehmannia root, Angelica root, and wolfberry fruit evenly, and grind them into powder to form a mixed powder;

[0011] S3. Mix zinc-enriched edible yeast powder, 300-500 parts by weight of soy protein isolate, 500-1000 parts by weight of concentrated whey protein and 1000 parts by weight of purified water evenly, and incubate at a constant temperature of 30-35℃ for 6-8 hours to form a slurry-like colony proliferation medium.

[0012] S4. Mix the mixed powder, 100 parts by weight of purified water, 50 parts by weight of soy protein isolate, 50 parts by weight of concentrated whey protein and 100-200 parts by weight of Clostridium butyricum dry powder evenly, and incubate at a constant temperature of 35-37℃ for 24-36 hours to obtain activated Clostridium butyricum sludge.

[0013] S5. Place the activated Clostridium butyricum sludge into a slurry-like colony proliferation medium and incubate at 35-37℃ for 24-36 hours to obtain a microbial-rich fermented mixture.

[0014] S6. Mix xanthan gum and fructooligosaccharides in the remaining activated water to form a mixture. Place the fermented mixture, L-carnitine, vitamin E powder, folic acid, taurine, oyster powder, cordyceps militaris powder, remaining soy protein isolate, remaining whey protein concentrate, and sorbitol into a mixer and dry mix at 30 rpm for 10-15 minutes. After dry mixing, pour the mixture into the dry powder mixture and blend at 60 rpm for 20-30 minutes to obtain a wet blend.

[0015] S7. Dry the wet blend and pulverize the dried material to obtain a microecological preparation composition.

[0016] Preferably, in step S2, the mixed powder is passed through a 30-mesh sieve.

[0017] Preferably, in step S5, after the activated Clostridium butyricum sludge is placed into the slurry-like colony proliferation medium, stirring is performed to accelerate the dispersion of the activated Clostridium butyricum sludge in the slurry-like colony proliferation medium.

[0018] Preferably, in step S7, the drying temperature is below 45°C, and the moisture content after drying is below 2%wt.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] This invention uses a mixture of Phellodendron bark, Anemarrhena rhizome, Epimedium, Achyranthes root, Rehmannia root, Angelica sinensis, and Lycium barbarum fruit to prepare a powder. A slurry-like culture medium for bacterial proliferation is prepared using zinc-enriched edible yeast powder, soy protein isolate, concentrated whey protein, and purified water. An activated Clostridium butyricum mycelium sludge is prepared using the mixed powder, Clostridium butyricum powder, purified water, soy protein isolate, and concentrated whey protein. After fermentation, the activated Clostridium butyricum mycelium sludge is mixed with the slurry-like culture medium to amplify Clostridium butyricum, resulting in a microbial-enriched fermentation mixture. Finally, a mixture of xanthan gum, fructooligosaccharides, and purified water is added to the microbial-enriched fermentation mixture. L-carnitine, vitamin E powder, folic acid, taurine, oyster shell powder, cordyceps militaris powder, remaining soy protein isolate, remaining whey protein concentrate, and sorbitol are mixed into a dry powder to obtain a wet blend. Drying the wet blend yields a microecological preparation composition. Adding this microecological preparation composition to feed can effectively improve feed palatability and nutrient utilization, promote feed intake and digestion in livestock and poultry, improve the intestinal microbial environment of livestock and poultry, enhance the immunity of livestock and poultry, improve the overall physical indicators of livestock and poultry, improve the quality of livestock and poultry semen without overtaxing them, and also help extend the lifespan of livestock and poultry. Attached Figure Description

[0021] Figure 1 This is a flowchart of the method for preparing the Clostridium butyricum microecological preparation composition of the present invention. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Example 1:

[0024] Please see Figure 1As shown, a Clostridium butyricum microecological preparation composition for improving the sperm quality of livestock and poultry includes the following raw materials in parts by weight: 10 parts of Phellodendron bark, 5 parts of Anemarrhena rhizome, 1 part of Epimedium, 10 parts of Achyranthes bidentata root, 5 parts of Rehmannia glutinosa root, 5 parts of Angelica sinensis root, 1 part of Lycium barbarum fruit, 200 parts of L-carnitine, 3 parts of vitamin E powder, 1 part of folic acid, 10 parts of taurine, 30 parts of oyster shell powder, 150 parts of Cordyceps militaris powder, 1500 parts of soy protein isolate, 4500 parts of whey protein concentrate, 30 parts of zinc-enriched edible yeast powder, 2000 parts of sorbitol, 50 parts of xanthan gum, 30 parts of fructooligosaccharides, 2000 parts of purified water, and 100 parts of Clostridium butyricum dry powder.

[0025] Phellodendron bark has the effect of clearing kidney fire (ministerial fire) and reducing deficiency heat; Anemarrhena rhizome has the effect of nourishing kidney yin and moistening dryness and lubricating the intestines; Epimedium is a substance extracted from Epimedium and has the effect of tonifying kidney yang; Achyranthes bidentata contains a large amount of alkaloids, which can nourish the liver and kidneys and strengthen muscles and bones; Rehmannia glutinosa can nourish yin and blood, benefit essence and fill marrow, and is used to treat liver and kidney yin deficiency; Angelica sinensis has the function of regulating blood and nourishing blood; Lycium barbarum can nourish the liver and kidneys. These Chinese medicines all use natural ingredients and can promote the increase of qi and blood in livestock and poultry to a certain extent, and nourish the kidney yang of livestock and poultry. Adding L-carnitine to feed can improve feed conversion rate.

[0026] Vitamin E possesses antioxidant and anti-inflammatory biological activities, especially in scavenging free radicals and blocking lipid oxidation. Adding vitamin E to the composition can prevent the increase of free radicals and ROS in livestock and poultry semen, reduce oxidative stress, reduce the peroxidation of unsaturated fatty acids in sperm membrane and acrosome membrane structures, thereby increasing semen viability and enhancing semen fertilization potential.

[0027] Folic acid can be used for energy and protein metabolism, purine and DNA synthesis, thereby improving the reproductive performance of livestock and poultry. Taurine can enhance the antioxidant capacity of cell membranes and improve sperm motility to a certain extent.

[0028] Oysters have the effects of nourishing blood, replenishing blood, and nourishing yin. Cordyceps militaris powder benefits the lungs and kidneys. The combination of the two can nourish the qi and blood of livestock and poultry. Soy protein isolate and whey protein concentrate are used to provide the protein content of the composition. Zinc-rich edible yeast powder and Clostridium butyricum dry powder fermentation products can effectively improve the palatability of the composition. Moreover, after the microorganisms enter the intestines of livestock and poultry, they can effectively improve the intestinal environment of livestock and poultry, promote the absorption of nutrients by livestock and poultry, and help improve the physical condition of livestock and poultry. Xanthan gum and fructooligosaccharides are used to bind the composition and improve the palatability of the composition.

[0029] The method for preparing the Clostridium butyricum microecological preparation composition includes the following steps:

[0030] S1. Weigh each group of raw materials according to the weight ratio and set aside;

[0031] S2. Mix Phellodendron bark, Anemarrhena rhizome, Epimedium, Achyranthes root, Rehmannia root, Angelica root, and wolfberry fruit evenly, and grind them into powder to form a mixed powder. Pass the mixed powder through a 30-mesh sieve.

[0032] S3. Mix zinc-enriched edible yeast powder, 300 parts by weight of soy protein isolate, 500 parts by weight of concentrated whey protein and 1000 parts by weight of purified water evenly, and incubate at 30°C for 6 hours to form a slurry-like colony proliferation medium.

[0033] S4. Mix the mixed powder, 100 parts by weight of purified water, 50 parts by weight of soy protein isolate, 50 parts by weight of concentrated whey protein and 100 parts by weight of Clostridium butyricum dry powder evenly, and incubate at 35°C for 24 hours to obtain activated Clostridium butyricum sludge.

[0034] S5. Place the activated Clostridium butyricum sludge into the slurry colony proliferation medium, stir to accelerate the dispersion of the activated Clostridium butyricum sludge in the slurry colony proliferation medium, and incubate at 35℃ for 24 hours to obtain a microbial-rich fermentation mixture.

[0035] S6. Mix xanthan gum and fructooligosaccharides in the remaining activated water to form a mixture. Place the fermented mixture, L-carnitine, vitamin E powder, folic acid, taurine, oyster powder, cordyceps militaris powder, remaining soy protein isolate, remaining whey protein concentrate, and sorbitol into a mixer and dry mix at 30 rpm for 10 minutes. After dry mixing, pour the mixture into the dry powder mixture and blend at 60 rpm for 20 minutes to obtain a wet blend.

[0036] S7. Dry the wet blend and pulverize the dried material. The drying temperature is below 45℃, and the moisture content after drying is below 2%wt, to obtain the microecological preparation composition.

[0037] During use, the microecological preparation composition can be mixed with feed at a ratio of 1:3, which can effectively improve the vitality of livestock and poultry, and at the same time improve the sperm quality of livestock and poultry.

[0038] Example 2:

[0039] Please see Figure 1As shown, a Clostridium butyricum microecological preparation composition for improving the sperm quality of livestock and poultry includes the following raw materials in parts by weight: 12 parts of Phellodendron bark, 10 parts of Anemarrhena rhizome, 3 parts of Epimedium, 15 parts of Achyranthes bidentata root, 10 parts of Rehmannia glutinosa root, 10 parts of Angelica sinensis root, 5 parts of Lycium barbarum fruit, 250 parts of L-carnitine, 6 parts of vitamin E powder, 3 parts of folic acid, 12 parts of taurine, 50 parts of oyster shell powder, 200 parts of Cordyceps militaris powder, 2000 parts of soy protein isolate, 5000 parts of whey protein concentrate, 50 parts of zinc-enriched edible yeast powder, 2500 parts of sorbitol, 70 parts of xanthan gum, 50 parts of fructooligosaccharides, 3000 parts of purified water, and 200 parts of Clostridium butyricum dry powder.

[0040] Phellodendron bark has the effect of clearing kidney fire (ministerial fire) and reducing deficiency heat; Anemarrhena rhizome has the effect of nourishing kidney yin and moistening dryness and lubricating the intestines; Epimedium is a substance extracted from Epimedium and has the effect of tonifying kidney yang; Achyranthes bidentata contains a large amount of alkaloids, which can nourish the liver and kidneys and strengthen muscles and bones; Rehmannia glutinosa can nourish yin and blood, benefit essence and fill marrow, and is used to treat liver and kidney yin deficiency; Angelica sinensis has the function of regulating blood and nourishing blood; Lycium barbarum can nourish the liver and kidneys. These Chinese medicines all use natural ingredients and can promote the increase of qi and blood in livestock and poultry to a certain extent, and nourish the kidney yang of livestock and poultry. Adding L-carnitine to feed can improve feed conversion rate.

[0041] Vitamin E possesses antioxidant and anti-inflammatory biological activities, especially in scavenging free radicals and blocking lipid oxidation. Adding vitamin E to the composition can prevent the increase of free radicals and ROS in livestock and poultry semen, reduce oxidative stress, reduce the peroxidation of unsaturated fatty acids in sperm membrane and acrosome membrane structures, thereby increasing semen viability and enhancing semen fertilization potential.

[0042] Folic acid can be used for energy and protein metabolism, purine and DNA synthesis, thereby improving the reproductive performance of livestock and poultry. Taurine can enhance the antioxidant capacity of cell membranes and improve sperm motility to a certain extent.

[0043] Oysters have the effects of nourishing blood, replenishing blood, and nourishing yin. Cordyceps militaris powder benefits the lungs and kidneys. The combination of the two can nourish the qi and blood of livestock and poultry. Soy protein isolate and whey protein concentrate are used to provide the protein content of the composition. Zinc-rich edible yeast powder and Clostridium butyricum dry powder fermentation products can effectively improve the palatability of the composition. Moreover, after the microorganisms enter the intestines of livestock and poultry, they can effectively improve the intestinal environment of livestock and poultry, promote the absorption of nutrients by livestock and poultry, and help improve the physical condition of livestock and poultry. Xanthan gum and fructooligosaccharides are used to bind the composition and improve the palatability of the composition.

[0044] The method for preparing the Clostridium butyricum microecological preparation composition includes the following steps:

[0045] S1. Weigh each group of raw materials according to the weight ratio and set aside;

[0046] S2. Mix Phellodendron bark, Anemarrhena rhizome, Epimedium, Achyranthes root, Rehmannia root, Angelica root, and wolfberry fruit evenly, and grind them into powder to form a mixed powder. Pass the mixed powder through a 30-mesh sieve.

[0047] S3. Mix zinc-enriched edible yeast powder, 500 parts by weight of soy protein isolate, 1000 parts by weight of concentrated whey protein and 1000 parts by weight of purified water evenly, and incubate at 35°C for 8 hours to form a slurry-like colony proliferation medium.

[0048] S4. Mix the mixed powder, 100 parts by weight of purified water, 50 parts by weight of soy protein isolate, 50 parts by weight of concentrated whey protein and 200 parts by weight of Clostridium butyricum dry powder evenly, and incubate at 37°C for 36 hours to obtain activated Clostridium butyricum sludge.

[0049] S5. Place the activated Clostridium butyricum sludge into the slurry colony proliferation medium, stir to accelerate the dispersion of the activated Clostridium butyricum sludge in the slurry colony proliferation medium, and incubate at 37℃ for 36 hours to obtain a microbial-rich fermentation mixture.

[0050] S6. Mix xanthan gum and fructooligosaccharides in the remaining activated water to form a mixture. Place the fermented mixture, L-carnitine, vitamin E powder, folic acid, taurine, oyster powder, cordyceps militaris powder, remaining soy protein isolate, remaining whey protein concentrate, and sorbitol into a mixer for dry mixing at 30 rpm for 15 minutes. After dry mixing, pour the mixture into the dry powder mixture for co-mixing at 60 rpm for 30 minutes to obtain a wet mixture.

[0051] S7. Dry the wet blend and pulverize the dried material. The drying temperature is below 45℃, and the moisture content after drying is below 2%wt, to obtain the microecological preparation composition.

[0052] During use, the microecological preparation composition can be mixed with feed at a ratio of 1:3, which can effectively improve the vitality of livestock and poultry, and at the same time improve the sperm quality of livestock and poultry.

[0053] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0054] The accompanying drawings of the embodiments disclosed in this invention only involve structures relevant to the embodiments disclosed in this invention. Other structures can be referred to with common designs. Unless otherwise specified, the same embodiment and different embodiments of this invention can be combined with each other.

Claims

1. A Clostridium butyricum microecological preparation composition for improving sperm quality in livestock and poultry, characterized in that, The ingredients include the following parts by weight: 10-12 parts of Phellodendron bark, 5-10 parts of Anemarrhena rhizome, 1-3 parts of Epimedium, 10-15 parts of Achyranthes root, 5-10 parts of Rehmannia root, 5-10 parts of Angelica sinensis, 1-5 parts of Lycium barbarum fruit, 200-250 parts of L-carnitine, 3-6 parts of vitamin E powder, 1-3 parts of folic acid, 10-12 parts of taurine, 30-50 parts of oyster shell powder, 150-200 parts of Cordyceps militaris powder, 1500-2000 parts of soy protein isolate, 4500-5000 parts of whey protein concentrate, 30-50 parts of zinc-enriched edible yeast powder, 2000-2500 parts of sorbitol, 50-70 parts of xanthan gum, 30-50 parts of fructooligosaccharides, 2000-3000 parts of purified water, and 100-200 parts of Clostridium butyricum powder.

2. The Clostridium butyricum microecological preparation composition for improving sperm quality in livestock and poultry according to claim 1, characterized in that, The method for preparing the Clostridium butyricum microecological preparation composition includes the following steps: S1. Weigh each group of raw materials according to the weight ratio and set aside; S2. Mix Phellodendron bark, Anemarrhena rhizome, Epimedium, Achyranthes root, Rehmannia root, Angelica root, and wolfberry fruit evenly, and grind them into powder to form a mixed powder; S3. Mix zinc-enriched edible yeast powder, 300-500 parts by weight of soy protein isolate, 500-1000 parts by weight of concentrated whey protein and 1000 parts by weight of purified water evenly, and incubate at a constant temperature of 30-35℃ for 6-8 hours to form a slurry-like colony proliferation medium. S4. Mix the mixed powder, 100 parts by weight of purified water, 50 parts by weight of soy protein isolate, 50 parts by weight of concentrated whey protein and 100-200 parts by weight of Clostridium butyricum dry powder evenly, and incubate at a constant temperature of 35-37℃ for 24-36 hours to obtain activated Clostridium butyricum sludge. S5. Place the activated Clostridium butyricum sludge into a slurry-like colony proliferation medium and incubate at 35-37℃ for 24-36 hours to obtain a microbial-rich fermented mixture. S6. Mix xanthan gum and fructooligosaccharides in the remaining activated water to form a mixture. Place the fermented mixture, L-carnitine, vitamin E powder, folic acid, taurine, oyster powder, cordyceps militaris powder, remaining soy protein isolate, remaining whey protein concentrate, and sorbitol into a mixer and dry mix at 30 rpm for 10-15 minutes. After dry mixing, pour the mixture into the dry powder mixture and blend at 60 rpm for 20-30 minutes to obtain a wet blend. S7. Dry the wet blend and pulverize the dried material to obtain a microecological preparation composition.

3. The Clostridium butyricum microecological preparation composition for improving sperm quality in livestock and poultry according to claim 2, characterized in that: In step S2, the mixed powder is passed through a 30-mesh sieve.

4. The Clostridium butyricum microecological preparation composition for improving sperm quality in livestock and poultry according to claim 3, characterized in that: In step S5, after the activated Clostridium butyricum sludge is placed into the slurry-like colony proliferation medium, stirring is performed to accelerate the dispersion of the activated Clostridium butyricum sludge in the slurry-like colony proliferation medium.

5. The Clostridium butyricum microecological preparation composition for improving sperm quality in livestock and poultry according to claim 4, characterized in that: In S7, the drying temperature is below 45°C, and the moisture content after drying is below 2%wt.

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