Permeation enhancing composition for improving skin feeling and application and cosmetic thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses a permeation enhancing composition for improving skin feeling, application of the permeation enhancing composition and cosmetics. The permeation enhancing composition is prepared from the following components: hydroxyethyl piperazine ethanesulfonic acid, inositol, bis-diethoxy diethylene glycol cyclohexane 1, 4-dicarboxylate, polyquaternium and hydrolyzed sodium hyaluronate; the mass ratio of the hydroxyethyl piperazine ethanesulfonic acid to the inositol to the bis-diethoxy diethylene glycol cyclohexane 1, 4-dicarboxylate to the polyquaternium to the hydrolyzed sodium hyaluronate is (0.01 to 10): (0.01 to 10): (0.01 to 10): (0.001 to 8): (0.001 to 5); the components are reasonably compounded, so that the permeation enhancing composition disclosed by the invention fully exerts the synergistic interaction effect among the components, has the characteristics of high safety, good skin feeling, mildness and efficient permeation enhancing, can remarkably improve the permeation enhancing effect, and has a wide application prospect in the field of cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and discloses a penetration-enhancing composition, its uses, and cosmetics. Background Technology

[0002] The main obstacle to the efficacy of transdermal drug delivery systems is the barrier effect of the skin barrier. To improve drug absorption efficiency, a certain amount of penetration enhancer is generally added to enhance drug penetration. The mechanism of action of penetration enhancers is mainly through dissolving skin lipids or denaturing skin proteins, increasing the disorder of the lipid backbone, thereby reversibly weakening the barrier function of the stratum corneum. This process generally does not damage living cells, but can effectively promote drug diffusion in the stratum corneum, increase drug solubility in the skin, and thus improve the transdermal absorption rate. Currently, commonly used penetration enhancement techniques are mainly divided into two categories: chemical penetration enhancement and physical penetration enhancement. Among them, chemical penetration enhancement refers to increasing drug absorption by adding penetration enhancers, and is currently the main means of promoting transdermal drug absorption. Commonly used chemical penetration enhancers include laurocapram and its analogues, organic acids and their esters (such as oleic acid), pyrrolidone derivatives, solvents (such as dimethyl sulfoxide, propylene glycol, and ethanol), and natural products (such as eucalyptus oil and peppermint oil).

[0003] However, existing penetration enhancers all have certain limitations. Azone-based penetration enhancers have drawbacks such as slow onset of action and poor penetration enhancement for lipophilic active ingredients; organic acid and ester-based penetration enhancers have drawbacks such as strong irritation, questionable long-term safety, strong concentration dependence, and poor solubility and compatibility; pyrrolidone derivatives have shortcomings such as low efficiency in action on hydrophilic and macromolecular drugs and nonlinear concentration-dependent effects; most solvent-based penetration enhancers require high concentrations to achieve significant penetration enhancement effects (e.g., dimethyl sulfoxide requires >60%), but high concentrations can easily lead to skin barrier damage, potentially causing irritation reactions such as erythema, edema, and burning sensation; natural product-based penetration enhancers generally have strong odors and are subject to certain limitations in practical applications.

[0004] In the field of daily chemical products, the selection of penetration enhancers is particularly crucial. This field requires penetration enhancers to be safe, mild, and effective. Furthermore, since daily chemical products often contain multiple active ingredients, it is often necessary to combine penetration enhancers with different mechanisms of action to achieve optimal penetration. However, existing penetration enhancers struggle to achieve an ideal balance between safety, skin feel, and penetration efficiency. Therefore, the technical problem this invention aims to solve is how to provide a highly safe, skin-friendly, mild, and highly effective penetration enhancer composition to meet the high performance requirements of penetration enhancers in the daily chemical product industry. Summary of the Invention

[0005] The purpose of this invention is to provide a penetration-enhancing composition that improves skin feel. By rationally compounding hydroxyethylpiperazine ethane sulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt, and hydrolyzed sodium hyaluronate, the synergistic effect between the components is utilized to achieve a high level of safety, good skin feel, gentleness, and high efficiency in enhancing penetration.

[0006] In addition, the present invention also provides the use of the penetration-enhancing composition and cosmetics containing the composition.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] A skin-enhancing composition for improving skin feel, the composition comprising the following components: hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene cyclohexane 1,4-dicarboxylate, polyquaternium salt, and hydrolyzed sodium hyaluronate; wherein the mass ratio of hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene cyclohexane 1,4-dicarboxylate, polyquaternium salt, and hydrolyzed sodium hyaluronate is 0.01-10:0.01-10:0.01-10:0.001-8:0.001-5.

[0009] In this invention, by compounding hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt, and hydrolyzed sodium hyaluronate, the composition can achieve comprehensive penetration enhancement by exfoliating the keratinocyte pathway, regulating the keratinocyte pathway, influencing the intercellular lipid pathway, and utilizing the skin appendage pathway. This results in a penetration-enhancing composition that is highly safe, has a good skin feel, and is gentle yet highly effective.

[0010] Preferably, the mass ratio of hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt and hydrolyzed sodium hyaluronate is 0.05-8:0.05-8:0.05-8:0.001-5:0.001-4.

[0011] More preferably, the mass ratio of hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt and hydrolyzed sodium hyaluronate is 0.5-4:0.5-3.5:0.5-2:0.001-3:0.002-2.

[0012] Preferably, the molecular weight of the hydrolyzed sodium hyaluronate is 3-600 kDa.

[0013] More preferably, the hydrolyzed sodium hyaluronate is sodium hyaluronate of full molecular weight; the molecular weight of the sodium hyaluronate of full molecular weight is 3-600 kDa, and the molecular weight dispersion coefficient Mw / Mn is 5.2-7.1.

[0014] More specifically, please refer to the patent application with publication number CN113512134A, which is entitled "Sodium hyaluronate with full molecular weight distribution and its preparation method and application". The sodium hyaluronate with full molecular weight prepared in Examples 1 to 6 of that patent application is applicable to the present invention.

[0015] Preferably, the full molecular weight sodium hyaluronate is one or more combinations of HA0.3, HA20, HA50, and HA60.

[0016] Preferably, the polyquaternary ammonium salt is polyquaternary ammonium salt-51.

[0017] Furthermore, this invention discloses the use of the penetration-enhancing composition described above in the preparation of cosmetics.

[0018] Finally, the present invention also discloses a cosmetic containing 1wt%-15wt% of the penetration-enhancing composition as described above.

[0019] Preferably, the dosage form of the cosmetic is one of lotion, cream, spray, serum, lotion, mask, and gel.

[0020] Compared with the prior art, the beneficial effects of the present invention are:

[0021] The skin-enhancing and penetration-promoting composition provided by this invention, through the rational combination of multiple effective ingredients such as hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt, and hydrolyzed sodium hyaluronate, fully utilizes the synergistic effect between the components to achieve a high level of safety, a pleasant skin feel, gentleness, and high efficiency in enhancing penetration. This composition may promote the transdermal absorption of drugs or active ingredients through multiple pathways, including the stratum corneum, keratinocytes, intercellular lipids, and skin appendages, demonstrating excellent application prospects. Attached Figure Description

[0022] Figure 1 This is an observation image of a chicken embryo before use of the permeation-enhancing composition of Example 1;

[0023] Figure 2 This is an observation image of chicken embryos after using the permeation-enhancing composition of Example 1;

[0024] Figure 3 This is an observation image of a chicken embryo before use of the permeation-enhancing composition in Example 2;

[0025] Figure 4 This is an observation image of chicken embryos after using the permeation-enhancing composition of Example 2;

[0026] Figure 5 This is an observation image of chicken embryos before use of the permeation-enhancing composition in Example 3;

[0027] Figure 6 This is an observation image of chicken embryos after using the permeation-enhancing composition of Example 3;

[0028] Figure 7 The graph shows the results of the relative expression levels of elastin in serums 1 and 9. Detailed Implementation

[0029] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0030] Product Information:

[0031] Hydroxyethylpiperazine ethane sulfonic acid: trade name Cosmecare 712; purchased from Hanning Chemical (Shanghai) Co., Ltd.

[0032] Inositol: Purchased from Shandong Huahui Biotechnology Co., Ltd.;

[0033] Bis-diethoxydiethylenecyclohexane 1,4-dicarboxylic acid ester: trade name Neosolue-Aqulio; purchased from NIPPON FINE CHEMICAL CO., LTD.;

[0034] Polyquaternium-51: Trade name Cellpolypid®-PMB8100E cell phospholipid aqueous solution; purchased from Shanghai Aoli Industrial Co., Ltd.

[0035] Hydrolyzed sodium hyaluronate 1: Trade name HA0.3; purchased from Shandong Guantianxia Biotechnology Co., Ltd.

[0036] Hydrolyzed Sodium Hyaluronate 2: Trade name: Sodium Hyaluronate-20 with full molecular weight distribution (version number 202203); purchased from Shandong Guantianxia Biotechnology Co., Ltd.

[0037] Hydrolyzed sodium hyaluronate 3: Trade name HA-50; purchased from Shandong Guantianxia Biotechnology Co., Ltd.

[0038] Hydrolyzed sodium hyaluronate 4: Trade name GD-HA60 (version number 202202); purchased from Shandong Guantianxia Biotechnology Co., Ltd.

[0039] Hydrolyzed Sodium Hyaluronate 5: Trade name: Ximinxiu™ Super Active Hyaluronic Acid; purchased from Bloomage Biotechnology Co., Ltd.

[0040] Hydrolyzed Sodium Hyaluronate 6: Trade name: Hydrolyzed Sodium Hyaluronate; purchased from Shandong Yinhe Biotechnology Co., Ltd.

[0041] Heparin sodium: Trade name is heparin sodium; purchased from Shandong Shenlian Pharmaceutical Co., Ltd.

[0042] Part One

[0043] The formulations of the penetration-enhancing compositions in each embodiment and comparative example are shown in Table 1:

[0044] Table 1. Formulation of Penetration Enhancing Compositions (parts by weight)

[0045] Hydroxyethylpiperazine ethanesulfonic acid Inositol bis-diethoxydiethylenecyclohexane 1,4-dicarboxylic acid ester Polyquaternium-51 Hydrolyzed sodium hyaluronate 1 Example 1 2.5 2 1.5 1.5 1 Example 2 0.5 3.5 0.5 3 0.1 Example 3 4 0.5 2 0.001 2 Comparative Example 1 0 3.4 2.6 1.5 1 Comparative Example 2 3.75 0 2.25 1.5 1 Comparative Example 3 3.33 2.67 0 1.5 1 Comparative Example 4 2.5 2 1.5 2.5 0 Comparative Example 5 2.5 2 1.5 0 2.5 Comparative Example 6 0 0 0 5.1 3.4 Comparative Example 7 3.58 2.86 2.16 0 0

[0046] Part Two: Discussion of the Application Performance of Different Types of Hydrolyzed Sodium Hyaluronate

[0047] The preparation method for this part is the same as that in Part 1, and the formulation and dosage are the same as in Example 1.

[0048] The sodium hyaluronate used in each embodiment is referenced in Table 2 below;

[0049] Table 2. Formulation of Penetration Enhancing Composition

[0050] Types of hydrolyzed sodium hyaluronate Example 4 Hydrolyzed sodium hyaluronate 2 Example 5 Hydrolyzed sodium hyaluronate 3 Example 6 Hydrolyzed sodium hyaluronate 4 Example 7 Hydrolyzed sodium hyaluronate 5 Example 8 Hydrolyzed sodium hyaluronate 6

[0051] The penetration-enhancing compositions prepared in the above embodiments and comparative examples were added to the serum to obtain serums 1-15. The penetration-enhancing compositions corresponding to serums 1-8 are the penetration-enhancing compositions of Examples 1-8, and the penetration-enhancing compositions corresponding to serums 9-15 are the penetration-enhancing compositions of Comparative Examples 1-7.

[0052] The formula of the serum is shown in Table 3; its preparation method is as follows:

[0053] Step 1: Weigh the E-phase material, heat it to 70-80℃, stir until completely dissolved, and confirm that the material is dissolved until it is clear and transparent, then set aside for use;

[0054] Step 2: Weigh the F phase material, stir until completely dissolved, and set aside;

[0055] Step 3: Weigh the A phase material, add it to the emulsification pot, and stir until completely dissolved;

[0056] Step 3: Weigh the B phase material, add it to the emulsification pot under homogeneous conditions, and stir until completely wetted;

[0057] Step 4: Weigh the C phase material, add it to the emulsification pot, stir, heat to 80-85℃, and stir until completely dissolved;

[0058] Step 5: Weigh the D phase material, add it to the oil phase pot, stir, and heat to 80-85℃;

[0059] Step 6: While homogenizing the material in the oil phase pot, pump it into the emulsifying pot, homogenize and stir until emulsification is complete;

[0060] Step 7: Stir the emulsifying pot materials and cool them to 45°C. Add the F phase and G phase materials and stir until they are evenly mixed.

[0061] Step 8: After the physicochemical indicators pass the test, filter and discharge the material.

[0062] Table 3. Serum Formula Table

[0063]

[0064] Performance testing of penetration-enhancing compositions

[0065] Chicken embryo chorioallantoic membrane eye stimulation test

[0066] 1. Experimental Materials

[0067] The penetration-enhancing compositions of Examples 1-3;

[0068] Chicken embryos: SPF grade White Laihang chickens, purchased from Zhejiang Lihua Agricultural Technology Co., Ltd.;

[0069] Culture conditions: Incubator temperature 37.5℃, relative humidity 65%.

[0070] 2. Experimental Procedure

[0071] 2.1 CAM Preparation: Purchase 0-day-old chicken embryos and incubate them until 9 days old. Inspect and discard any defective embryos. Mark the location of the air cell on the surface of the normal chicken embryo eggshell, peel off part of the eggshell to expose the white egg membrane; carefully remove the inner membrane with tweezers, ensuring that the vascular membrane is not damaged.

[0072] 2.2 Endpoint scoring method: At least 6 chicken embryos in each group. 0.3 mL of the test substance was directly added to the CAM surface and the CAM reaction was observed. After 3 min of action, the test substance was gently rinsed off with physiological saline. The three reactions of bleeding, coagulation and vascular dissolution and their degree were observed within about 30 s after rinsing. The total score was moderate or above (total score ≥12). The test should be repeated once.

[0073] Calculate the endpoint score (ES): the score for each chicken embryo is the sum of the observed bleeding, coagulation and vascularization in each chicken embryo; ES = the mathematical sum of the scores of 6 chicken embryos.

[0074] The eye irritation of the test substances was classified according to the ES values ​​in Table 4.

[0075] Table 4 Evaluation of Endpoint Scoring Method Results

[0076] Finish line score Irritant Classification ES≤12 Non-irritating / mildly irritating 12 < ES < 16 moderate irritation ES≥16 Strongly irritating / corrosive

[0077] The test results are shown in Table 5 and Figures 1-6 As shown;

[0078] Table 5 Scoring Results

[0079] Stimulus rating Irritating Example 1 0 Non-irritating / mildly irritating Example 2 1 Non-irritating / mildly irritating Example 3 0 Non-irritating / mildly irritating

[0080] The results above show that the chicken embryo fraction in Examples 1-3 was less than 12, and the results were all non-irritating / mildly irritating.

[0081] Figure 1 This is an observation image of a chicken embryo before use of the penetration-enhancing composition of Example 1. Figure 2 This is an observation image of chicken embryos after using the permeation-enhancing composition of Example 1.

[0082] Figure 3 This is an observation image of a chicken embryo before use of the penetration-enhancing composition in Example 2. Figure 4 This is an observation image of chicken embryos after using the permeation-enhancing composition of Example 2.

[0083] Figure 5 This is an observation image of a chicken embryo before use of the permeation-enhancing composition in Example 3. Figure 6 This is an observation image of chicken embryos after using the permeation-enhancing composition of Example 3.

[0084] Percutaneous osmosis test

[0085] 1 g of heparin sodium was added to the permeation-enhancing compositions of Examples 1-8 and Comparative Examples 1-7, and deionized water was added to a final volume of 20 g to investigate the effect of the permeation-enhancing compositions of Examples 1-8 and Comparative Examples 1-7 on the transdermal permeation-enhancing effect of heparin sodium. The experimental method is as follows:

[0086] Preparation of excised pig skin: Abdominal skin was immediately harvested from Bama miniature pigs after euthanasia. The subcutaneous fat layer and connective tissue were carefully peeled off, rinsed thoroughly with physiological saline, and stored in physiological saline at a low temperature for later use. Before the experiment, the skin was thawed naturally, soaked in physiological saline for 30 minutes, and then blotted dry with filter paper before use.

[0087] Transdermal permeation determination: The diffusion instrument's heating module was set to 37℃ and preheated. The aspiration head was placed in prepared physiological saline to clean the receiving cell and tubing. After completion, according to the instructions, detached pigskin discs were inserted, with the stratum corneum facing upwards, thoroughly flattened, and pressed tightly. Air bubbles were removed from the receiving cell. Samples from each group were aspirated and added to the drug delivery cell of the diffusion instrument, 300 mg per well, and evenly applied to the skin surface. 19.5 mL of receiving solution (physiological saline) was added to the receiving cell. After 24 h, the diffusion instrument automatically sampled 1 mL and replenished with the same volume of fresh medium at the same temperature. After sample removal, the heparin sodium content in the sample was analyzed by ultraviolet spectrophotometry, and the cumulative permeation per unit area over 24 h was calculated. Each sample was tested in triplicate, and the results were taken as the average of the three tests.

[0088] Intradermal retention of heparin sodium: After the percutaneous permeation test, the excised pig skin in the diffusion apparatus was removed, washed three times with physiological saline, dried with absorbent paper, cut into small pieces and placed in a centrifuge tube, then added pre-cooled physiological saline and cryogenically ground. After filtration at 0.22 μm, the content of heparin sodium was determined by ultraviolet spectrophotometry.

[0089] The detection method for heparin sodium is as follows: Take 0, 0.5, 1, 2, 3, 4, and 5 mL of the working solution of heparin sodium standard and pipette these solutions into 5 mL volumetric flasks. Add 1 mL of methylene blue working solution and 1 mL of physiological saline, and dilute to the mark with pure water to obtain standard solutions with final concentrations of 0, 0.5, 1, 2, 3, 4, and 5 μg / mL. After thorough mixing, allow the solution to stand in the dark for 1 h. After the reaction is complete, measure the absorbance at 664 nm within 15 min. Compare the absorbance with the absorbance at 0 concentration and plot a standard curve using the difference versus concentration. Take the sample to be tested, add methylene blue reaction solution, mix thoroughly, and then add pure water to the mark. Allow the solution to stand for 1 h and measure the absorbance. Calculate the heparin sodium content.

[0090] The test results are shown in Table 6.

[0091] Table 6 Test Results

[0092] <![CDATA[Heparin sodium content (μg / cm 2 )]]> Example 1 7.860 Example 2 7.201 Example 3 6.890 Example 4 7.314 Example 5 7.440 Example 6 6.958 Example 7 7.032 Example 8 7.655 Comparative Example 1 2.278 Comparative Example 2 2.138 Comparative Example 3 2.056 Comparative Example 4 1.673 Comparative Example 5 1.592 Comparative Example 6 1.975 Comparative Example 7 1.457

[0093] Analyzing Table 4, we can conclude that:

[0094] 1. Data from Examples 1, 6, and 7 show that when the combination of hydroxyethylpiperazine ethanesulfonic acid, inositol, and bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester or the combination of polyquaternium-51 and hydrolyzed sodium hyaluronate is missing from the penetration-enhancing composition, the penetration-enhancing effect of the composition is significantly reduced, far lower than that of Example 1; indicating that when the above two combinations are used together, they have a synergistic effect of increasing the penetration-enhancing effect of the composition.

[0095] 2. As can be seen from the data of Examples and Comparative Examples 1-3, when the combination of polyquaternium-51 and hydrolyzed sodium hyaluronate is present, the penetration-enhancing effect of the composition is significantly reduced when any one of the components, namely hydroxyethylpiperazine ethanesulfonic acid, inositol, and bis-diethoxydiethylene cyclohexane 1,4-dicarboxylate, is missing, which is much less than that in Example 1. This indicates that the three substances, namely hydroxyethylpiperazine ethanesulfonic acid, inositol, and bis-diethoxydiethylene cyclohexane 1,4-dicarboxylate, must be used simultaneously to have a synergistic effect in increasing the penetration-enhancing effect of the composition.

[0096] 3. As can be seen from the data of Examples 1 and Comparative Examples 4-5, when the combination containing hydroxyethylpiperazine ethanesulfonic acid, inositol, and bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester is missing either polyquaternium-51 or hydrolyzed sodium hyaluronate, the penetration-enhancing effect of the composition is significantly reduced, much less than that of Example 1. This indicates that polyquaternium-51 and hydrolyzed sodium hyaluronate must be used simultaneously to achieve a synergistic effect in increasing the penetration-enhancing effect of the composition.

[0097] 4. Data from Examples 1 and 4-8 show that the different types of hydrolyzed sodium hyaluronate used in the permeation-enhancing compositions all exhibited good permeation-enhancing effects, indicating that each type of hydrolyzed sodium hyaluronate can synergistically enhance permeation-enhancing efficacy with other components.

[0098] In summary, in this invention, only by rationally combining hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt, and hydrolyzed sodium hyaluronate can the penetration-enhancing effect of the penetration-enhancing composition be synergistically increased.

[0099] Application Example Test

[0100] To evaluate the anti-aging effects of serum 1 and serum 9 prepared in this invention, elastin was used as an indicator for detection.

[0101] Elastin is a key sclerosing protein in human connective tissue, playing a crucial role, especially in the elastic tissues of skin, tendons, and arteries. Its integrity is essential for maintaining skin elasticity and suppleness. Loss of skin elasticity is primarily caused by the breakdown of fibers such as elastin, which leads to the degradation of the extracellular matrix, thus accelerating the skin aging process. Therefore, detecting the elastin content in the extracellular matrix of human dermal fibroblasts using ELISA can effectively assess the anti-aging effects of serums.

[0102] The anti-aging effects of serums 1 and 9 prepared according to this invention were evaluated. Following the serum preparation method, the penetration-enhancing composition in the serum was replaced with an equal weight of water, while other steps remained unchanged, serving as a blank serum control group.

[0103] Human dermal fibroblasts in the logarithmic growth phase were at a density of 5×10⁻⁶. 5Cells were seeded at a density of 10 cells / mL into 24-well plates and cultured for 24 h. Cells nearing confluence were then synchronized by switching to serum-free DMEM medium and starving for 2 h. 10 ng / mL human epidermal growth factor (HGF) was used as a positive control, and untreated cells as a negative control. Cells were cultured for 48 h after HGF administration, with three replicates per group. Cell culture supernatant was collected for later use. The elastin ELISA kit was administered according to the manufacturer's instructions, and the absorbance at 450 nm was measured using a microplate reader. The elastin content in the extracellular matrix of human dermal fibroblasts was calculated based on the plotted standard curve. Results are as follows: Figure 7 As shown;

[0104] Depend on Figure 7 It was found that human epidermal growth factor at a concentration of 10 ng / mL, used as a positive control, significantly promoted elastin expression, with the relative expression level increasing to 115.93% (P<0.05). After treatment with the blank serum, the relative expression level of elastin in the extracellular matrix was 98.68%, which was not significantly different from the negative control group (P>0.05). After treatment with serum 9, the relative expression level of elastin in the extracellular matrix was 101.32%, which was not significantly different from the negative control group (P>0.05). However, compared with the negative control group, after treatment with serum 1, the relative expression level of elastin in the extracellular matrix was significantly increased (P<0.01), with a relative expression level of 112.65%. Therefore, among the blank serum, serum 1, and serum 9, only serum 1 could significantly promote the expression of elastin in fibroblasts, demonstrating clear anti-aging potential.

[0105] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

Claims

1. A penetration-enhancing composition for improving skin feel, characterized in that, The penetration-enhancing composition comprises the following components: hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt, and hydrolyzed sodium hyaluronate; wherein the mass ratio of hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt, and hydrolyzed sodium hyaluronate is 0.01-10:0.01-10:0.01-10:0.001-8:0.001-5.

2. The penetration-enhancing composition according to claim 1, characterized in that, The mass ratio of the hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt and hydrolyzed sodium hyaluronate is 0.05-8:0.05-8:0.05-8:0.001-5:0.001-4.

3. The penetration-enhancing composition according to claim 2, characterized in that, The mass ratio of the hydroxyethylpiperazine ethanesulfonic acid, inositol, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester, polyquaternium salt and hydrolyzed sodium hyaluronate is 0.5-4:0.5-3.5:0.5-2:0.001-3:0.002-2.

4. The penetration-enhancing composition according to claim 1, characterized in that, The molecular weight of the hydrolyzed sodium hyaluronate is 3-600 kDa.

5. The penetration-enhancing composition according to claim 1, characterized in that, The hydrolyzed sodium hyaluronate is sodium hyaluronate of full molecular weight; the molecular weight of the sodium hyaluronate of full molecular weight is 3-600 kDa, and the molecular weight dispersion coefficient Mw / Mn is 5.2-7.

1.

6. The penetration-enhancing composition according to claim 1, characterized in that, The polyquaternary ammonium salt is polyquaternary ammonium salt-51.

7. Use of the penetration-enhancing composition according to any one of claims 1-6 in the preparation of cosmetics.

8. A cosmetic product, characterized in that, The cosmetic contains 1wt%-15wt% of the penetration-enhancing composition as described in claims 1-6.

9. The cosmetic product according to claim 8, characterized in that, The cosmetic product is in one of the following forms: lotion, cream, spray, serum, toner, mask, or gel.

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