Electrically-driven magnetic rotary stirring device

Abstract

The invention provides an electrically-driven magnetic rotary stirring device. The electrically-driven magnetic rotary stirring device comprises a magneton rotating structure, a fixed plate, a driving motor and a magnet, the magneton rotating structure is fixed on the fixed plate, the magnet is fixed on a rotating shaft of the driving motor, the driving motor drives the magnet to rotate through the rotating shaft so as to generate a rotating magnetic field, the magneton rotating structure is provided with a rotatable magneton, the magneton is located in the rotating magnetic field generated by the magnet, and the magneton is driven by the rotating magnetic field of the magnet to rotate. According to the device, the direct-current motor drives the magnet to generate a rotating magnetic field, the magneton is arranged in the rotating magnetic field generated by the magnet, meanwhile, the magneton is soaked in the cell culture fluid, and the rotating magnetic field generated by the magnet drives the magneton to rotate to stir the cell culture fluid so as to form the effect of a fluid shear force microenvironment; the cell culture medium can be well adapted to and applied to cell culture plates, including but not limited to 48-pore plates, 24-pore plates, 12-pore plates, 6-pore plates and the like.

Description

[0001] This application is a divisional application of application number 202110506385.9, filed on May 10, 2021, entitled "An electrically driven magnetic rotary stirring device". Technical Field

[0002] This invention relates to the field of biological cell culture equipment, and more specifically, to an electrically driven magnetic rotary stirring device. Background Technology

[0003] Fluids are an essential component of every biological species. Under physiological conditions, many cell types are surrounded by a fluid environment. Typical examples include hematopoietic stem cells, vascular endothelial cells, lymphatic endothelial cells, kidney and lung epithelial cells, and suspended cells such as immune cells. This fluid flow induces shear stress, a mechanical force that affects cell morphology and behavior in a variety of ways. In many standard in vitro experiments, cells are cultured without flow. Under these static conditions, shear stress-dependent cell changes are typically not considered. In fact, it is meaningful to culture cells in vitro under flowing conditions and to simulate this mechanical stimulation to induce in vivo biological processes that more closely resemble physiological states. Simulating flow states is particularly important when studying cells present in biological fluids, such as endothelial cells or epithelial cells. Therefore, there is a need for an electrically driven magnetic stirring device based on well plates, including but not limited to 48-well, 24-well, 12-well, and 6-well plates, to provide a certain amount of fluid shear force to simulate the real in vivo environment during cell culture, thereby stimulating cell growth and differentiation. This would have considerable application value and provide a powerful tool for the field of cell culture.

[0004] Currently, research on this topic is relatively rare both domestically and internationally. Few researchers in the biological field have studied fluid shear stress devices. Some studies only involve large-scale stirring schemes without considering practically controllable small-scale environmental control. In addition, the implementation of large-scale fluid stirring devices and experiments requires significant costs. Summary of the Invention

[0005] The present invention provides an electrically driven magnetic rotary stirring device that provides fluid shear force for cell culture, thereby stimulating cell growth and differentiation.

[0006] To achieve the above-mentioned technical effects, the technical solution of the present invention is as follows: An electrically driven magnetic rotary stirring device includes a magnetic particle rotating structure, a fixed plate, a drive motor, and a magnet. The magnetic particle rotating structure is fixed on the fixed plate, and the magnet is fixed on the rotating shaft of the drive motor. The drive motor drives the magnet to rotate through the rotating shaft, thereby generating a rotating magnetic field. The magnetic particle rotating structure is provided with a rotatable magnetic particle, which is located in the rotating magnetic field generated by the magnet and rotates under the drive of the rotating magnetic field of the magnet.

[0007] In one embodiment, the magnetic rotating structure is fixed to the fixed plate by a nut; In another embodiment, the fixing plate has several perforations, through which the magnetic rotating structure is detachably fixed to the fixing plate.

[0008] Furthermore, a rudder disk is installed on the shaft of the drive motor, and several magnets are fixed on the rudder disk; In another embodiment, the steering wheel is provided with several latches, and magnets are engaged with the latches to achieve assembly and disassembly.

[0009] Furthermore, the device is equipped with a base plate, and the drive motor is fixed on the base plate; In another embodiment, the drive motor is detachably mounted on the base plate via a nut.

[0010] Preferably, the magnet is a circular strong magnetic magnet; the drive motor is a DC motor; and the magnet is detachably fixed to the magnet rotating structure.

[0011] The present invention also includes a method for stirring cell culture medium using an electrically driven magnetic rotary stirring device, wherein the electrically driven magnetic rotary stirring device is the electrically driven magnetic rotary stirring device according to any one of the preceding claims, and the method includes the following steps: (1) Soak and clean the fixed plate and the magnetic rotating structure in a 75% alcohol solution; (2) Mount the steering wheel onto the drive motor; (3) Assemble the magnet with the rudder; (4) Install the drive motors with the rudder and magnet already assembled onto the base plate in sequence; (5) Assemble and fix the drive motor to the base plate; (6) Test and adjust the rotation parameters and operating conditions of the drive motor through software; (7) After the cells adhere to the bottom of the well plate, turn on the electrically driven magnetic rotation device to rotate and stir the culture medium.

[0012] This device uses a DC motor to drive a magnet to generate a rotating magnetic field. A magnetic particle is placed in this rotating magnetic field and immersed in the cell culture medium. The rotating magnetic field drives the magnetic particle to rotate, stirring the cell culture medium and creating a fluid shear force microenvironment. This stimulates cell growth and differentiation. The device is well-suited for use with cell culture plates, including but not limited to 48-well, 24-well, 12-well, and 6-well plates, providing a powerful tool for the field of cell culture.

[0013] Compared with the prior art, the beneficial effects of the technical solution of the present invention are: 1. In many standard in vitro experiments, cells are cultured without flow. Under these static conditions, shear stress-dependent cell changes are typically not considered. In reality, it is meaningful to culture cells in vitro under flowing conditions and simulate this mechanical stimulation to induce in vivo biological processes that more closely resemble physiological states. This device allows for the provision of fluid shear forces during plate-based cell culture to simulate the real in vivo environment, thereby stimulating cell growth and differentiation, etc. 2. Compared with the prior art, the technical solution of this invention fully considers the creation and controllability of fluid shear force in small-scale cell culture experiments, and requires lower costs, providing a powerful tool for the field of cell culture. Attached Figure Description

[0014] Figure 1 This is a structural diagram of the device in Embodiment 1 of the present invention; Figure 2 This is a schematic diagram of the operation of the device in a specific experiment in Embodiment 1 of the present invention; Figure 3 This is a schematic diagram of the operation of the device in a specific experiment in Embodiment 2 of the present invention; Figure 4 This is a structural diagram of magneton rotation structure 1. Detailed Implementation

[0015] The accompanying drawings are for illustrative purposes only and should not be construed as limiting the scope of this patent. To better illustrate this embodiment, some parts in the accompanying drawings may be omitted, enlarged, or reduced, and do not represent the actual product dimensions; It will be understood by those skilled in the art that certain well-known structures and their descriptions may be omitted in the accompanying drawings.

[0016] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0017] Example 1 like Figure 1-2As shown, an electrically driven magnetic rotary stirring device includes a magnetic particle rotating structure 1, a fixed plate 2, a drive motor 3, and a magnet 4. The magnetic particle rotating structure 1 is fixed on the fixed plate 2, and the magnet 4 is fixed on the rotating shaft of the drive motor 3. The drive motor 3 drives the magnet 4 to rotate through the rotating shaft, thereby generating a rotating magnetic field. The magnetic particle rotating structure 1 is provided with a rotatable magnetic particle 5, which is located in the rotating magnetic field generated by the magnet 4. The magnetic particle 5 rotates under the drive of the rotating magnetic field of the magnet 4.

[0018] The fixing plate 2 has several perforations, and the magnetic rotating structure 1 is detachably fixed to the fixing plate 2 by passing through the perforations.

[0019] like Figure 4 As shown, a rudder disk 6 is mounted on the shaft of the drive motor 3, and several magnets 4 are fixed on the rudder disk 6.

[0020] The steering wheel 6 is equipped with several clips, and the magnet 4 is attached to the clips to enable assembly and disassembly.

[0021] The device is equipped with a base plate 7, and the drive motor 3 is fixed on the base plate 7; the drive motor 3 is detachably mounted on the base plate 7 by means of nuts.

[0022] Magnet 4 is a round, strong magnetic magnet; drive motor 3 is a DC motor; magnet 5 is detachably fixed on magnet rotating structure 1.

[0023] Example 2 like Figure 3 As shown, an electrically driven magnetic rotary stirring device includes a magnetic particle rotating structure 1, a fixed plate 2, a drive motor 3, and a magnet 4. The magnetic particle rotating structure 1 is fixed on the fixed plate 2, and the magnet 4 is fixed on the rotating shaft of the drive motor 3. The drive motor 3 drives the magnet 4 to rotate through the rotating shaft, thereby generating a rotating magnetic field. The magnetic particle rotating structure 1 is provided with a rotatable magnetic particle 5, which is located in the rotating magnetic field generated by the magnet 4. The magnetic particle 5 rotates under the drive of the rotating magnetic field of the magnet 4.

[0024] The magnetic rotating structure 1 is fixed to the fixed plate 2 by a nut; as shown Figure 4 As shown, a rudder disk 6 is mounted on the shaft of the drive motor 3, and several magnets 4 are fixed on the rudder disk 6.

[0025] The steering wheel 6 is equipped with several clips, and the magnet 4 is attached to the clips to enable assembly and disassembly.

[0026] The device is equipped with a base plate 7, and the drive motor 3 is fixed on the base plate 7; the drive motor 3 is detachably mounted on the base plate 7 by means of nuts.

[0027] Magnet 4 is a round, strong magnetic magnet; drive motor 3 is a DC motor; magnet 5 is detachably fixed on magnet rotating structure 1.

[0028] Example 3 The specific operation process of stirring the cell culture medium using the electrically driven magnetic rotary stirring device in Example 1 or 2 is as follows: (1) Soak and clean the fixed plate 2 and the magnetic rotating structure 1 in 75% alcohol solution, then place them in a clean bench under ultraviolet light and air dry. (2) Assemble the steering disk 6 onto the DC drive motor 3 to complete the assembly and fixation; (3) Assemble the magnet 4 and the rudder disk 6 to complete the assembly and fixation; (4) Install the DC drive motors, which have been assembled with the rudder disk 6 and the magnet 4, onto the base plate 7 in sequence; (5) Assemble and fix the DC drive motor 3 to the base plate 7 to further improve the stability of the device during operation; (6) Test and adjust the rotation parameters and operating status of DC drive motor 3 through software; (7) Culture hematopoietic stem cells (using hematopoietic stem cells as an example only, including but not limited to hematopoietic stem cells, mesenchymal stem cells, etc.) in cell culture dishes until they proliferate to 500,000. Digest the cells with trypsin to prepare a cell suspension, and then add the cell suspension to a 12-well plate using a pipette. After the cells have been allowed to stand for 24 hours and adhere to the bottom of the well plate, turn on the electrically driven magnetic rotation device to rotate and stir the culture medium; (8) After 4 days of cell culture, observe the cell growth and proliferation. When the cells reach 85% fusion, remove the well plate and place it on a clean bench for subsequent characterization of cell proliferation, differentiation and other characteristics.

[0029] This device uses a DC motor to drive a magnet 4 to generate a rotating magnetic field. The magnet 5 is placed in the rotating magnetic field generated by the magnet 4 and immersed in the cell culture medium. The rotating magnetic field generated by the magnet 4 drives the magnet 5 to rotate and stir the cell culture medium, thereby creating a fluid shear force microenvironment. This stimulates cell growth and differentiation, providing a powerful tool for the field of cell culture.

[0030] During cell culture, the continuous rotation of the magnetic rotating structure 1 enhances the exchange and transfer of nutrients between the culture medium and the cells. Furthermore, the rotation creates a microenvironment with a certain fluid shear force through the stirring of the liquid. In in vitro cell culture under flowing conditions, the stimulation and induction of this fluid shear force makes the cells in this environment closer to the physiological in vivo environment, and also makes the biological processes of the cells cultured in this environment more practically significant.

[0031] The same or similar labels correspond to the same or similar parts; The positional relationships depicted in the accompanying drawings are for illustrative purposes only and should not be construed as limiting this patent. Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. An electrically driven magnetic rotary stirring device, characterized in that, It includes a magnetic rotating structure (1), a fixed plate (2), a drive motor (3), and a magnet (4); the magnetic rotating structure (1) is fixed on the fixed plate (2), the magnet (4) is fixed on the rotating shaft of the drive motor (3), the drive motor (3) drives the magnet (4) to rotate through the rotating shaft and thus generates a rotating magnetic field, the magnetic rotating structure (1) is provided with a rotatable magnetic particle (5), the magnetic particle (5) is in the rotating magnetic field generated by the magnet (4), and the magnetic particle (5) rotates under the drive of the rotating magnetic field of the magnet (4).

2. The electrically driven magnetic rotary stirring device according to claim 1, characterized in that, The magnetic rotating structure (1) is fixed to the fixed plate (2) by a nut.

3. The electrically driven magnetic rotary stirring device according to claim 1, characterized in that, The fixing plate (2) has several perforations, and the magnetic rotating structure (1) passes through the perforations of the fixing plate (2) and is detachably fixed to the fixing plate (2).

4. The electrically driven magnetic rotary stirring device according to claim 1, characterized in that, A rudder disk (6) is mounted on the shaft of the drive motor (3), and several magnets (4) are fixed on the rudder disk (6).

5. The electrically driven magnetic rotary stirring device according to claim 4, characterized in that, The steering wheel (6) is provided with several clips, and the magnet (4) is attached to the clips to achieve assembly and disassembly.

6. The electrically driven magnetic rotary stirring device according to claim 1, characterized in that, The device is equipped with a base plate (7), and the drive motor (3) is fixed on the base plate (7).

7. The electrically driven magnetic rotary stirring device according to claim 2, characterized in that, The drive motor (3) is detachably mounted on the base plate (7) by means of a nut.

8. The electrically driven magnetic rotary stirring device according to any one of claims 1-7, characterized in that, The magnet (4) is a circular strong magnetic magnet.

9. The electrically driven magnetic rotary stirring device according to any one of claims 1-7, characterized in that, The drive motor (3) is a DC motor.

10. The electrically driven magnetic rotary stirring device according to any one of claims 1-7, characterized in that, The magnet (5) is detachably fixed to the magnet rotating structure (1).

11. A method for stirring cell culture medium using an electrically driven magnetic rotary stirring device, wherein the electrically driven magnetic rotary stirring device is the electrically driven magnetic rotary stirring device according to any one of claims 1-10, the method comprising the following steps: (1) Soak and clean the fixing plate (2) and the magnetic rotating structure (1) in a 75% alcohol solution; (2) Mount the steering wheel (6) onto the drive motor (3); (3) Assemble the magnet (4) with the rudder (6); (4) Install the drive motor with the rudder disk (6) and magnet (4) assembled on the base plate (7) in sequence; (5) Assemble and fix the drive motor (3) to the base plate (7); (6) Test and adjust the rotation parameters and operating status of the drive motor (3) through software; (7) After the cells adhere to the bottom of the well plate, turn on the electrically driven magnetic rotation device to rotate and stir the culture medium.

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