Novel preparation method of agar culture medium

Abstract

The invention discloses a novel preparation method of an agar culture medium, belongs to the technical field of biological culture media, and particularly relates to a novel preparation method of an agar culture medium, which comprises the following steps: heating purified water until water bottom bubbles, adding agar culture medium dry powder, quickly stirring until no caking exists, and standing until the agar culture medium is completely dissolved. According to the novel preparation method of the agar culture medium, the problems that the agar culture medium is easy to cake and difficult to dissolve after meeting hot water are solved, and the technical effects that the heating and dissolving time of the agar culture medium is shortened, and the agar culture medium is dissolved more uniformly are achieved.

Description

Technical Field

[0001] This invention belongs to the field of biological culture medium technology, and particularly relates to a novel method for preparing agar culture medium. Background Technology

[0002] Agar medium is an indispensable basic material in fields such as microbiology, cell biology, food, and environmental testing. It is mainly composed of nutrients (such as peptone, beef extract, yeast extract, etc.), essential inorganic salts, growth factors, and agar powder as a solidifying agent. The conventional method for preparing agar medium usually involves mixing the above components with distilled water in a certain proportion, heating and boiling to completely dissolve the agar powder, then autoclaving to achieve a sterile state, and finally pouring it into culture vessels under sterile conditions to solidify.

[0003] However, traditional preparation processes have several inherent defects that limit their efficiency and effectiveness in scientific research and production: excessive pyrolysis of agar and changes in its physicochemical properties; partial hydrolysis of agar polysaccharides under repeated or excessive heating conditions, leading to decreased gel strength, increased freezing point, or poor gel transparency. This not only affects the physical stability of the culture medium but may also interfere with the observation and identification of microbial colony morphology (especially those with diffusive characteristics).

[0004] Therefore, there is an urgent need in this field to develop a new method for preparing agar medium that is simpler and more efficient to operate and can ensure the stability of agar gel performance. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention proposes a novel preparation method for agar culture medium, which solves the problems of agar culture medium easily clumping and being difficult to dissolve when exposed to hot water, thereby achieving the technical effect of shortening the heating and dissolving time of agar culture medium and making the agar culture medium dissolve more uniformly.

[0006] To achieve the above objectives, the present invention provides a novel method for preparing agar medium, comprising the following steps: heating purified water until bubbles appear at the bottom of the water, adding agar medium dry powder, stirring rapidly until there are no lumps, and letting it stand until completely dissolved.

[0007] Preferably, the purified water is heated to the point where bubbles appear at the bottom, specifically with the following conditions: the lower layer water temperature is 90°C, the middle layer water temperature is 88°C, and the upper layer water temperature is 86°C.

[0008] Preferably, the ratio of the agar medium powder to purified water is 5g:77~125ml.

[0009] Preferably, the agar medium powder is added at a rate of 1000 g / min.

[0010] Preferably, the rapid stirring time is 2-3 minutes.

[0011] Preferably, the settling time is 2 to 3 minutes.

[0012] Compared with the prior art, the present invention has the following advantages and technical effects: This invention provides a novel method for preparing agar culture medium, aiming to fundamentally solve the core technical problem of clumping and dissolution difficulties caused by agar gelation upon contact with hot water in traditional preparation processes. By optimizing raw material processing, dispersion processes, and temperature control strategies, this invention achieves rapid and uniform dissolution of agar in solution, significantly shortening the overall heating and dissolution time and improving the uniformity and quality stability of the final culture medium product. Specifically, in traditional methods, when agar powder is directly added to cold or hot water, the agar particles rapidly absorb water and swell. If stirring is insufficient or the temperature gradient is uneven, stubborn clumps easily form, with a gelled outer layer and a dry powder interior. These clumps not only hinder the transfer of heat and moisture to the interior, greatly prolonging the boiling time required for complete dissolution (usually requiring continuous stirring and boiling for 30 minutes), but even after prolonged heating, microscopic gel particles may remain, leading to uneven hardness and poor transparency after the culture medium solidifies, thus affecting the cultivation and observation of microorganisms. The core improvement of this invention lies in controlling the stirring speed throughout the heating process via temperature control, thereby breaking the tendency of agar particles to aggregate and promoting their uniform dispersion and efficient dissolution. This saves energy and time costs, eliminates visible and microscopic undissolved agar particles, and results in a clear and transparent culture medium with consistent gel strength, a smooth surface, and high transparency after solidification. This invention provides a new, efficient, stable, and easily standardized agar medium preparation method, particularly suitable for microbiological laboratories, fermentation industries, and quality control departments requiring rapid preparation, high quality, and large-scale production. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 These are photographs of dry powder agar medium, where A is a photograph of dry powder tryptic soy agar medium and B is a photograph of dry powder Sabouraud dextrose agar medium. Figure 2The images show the state of the culture medium when tryptic soy agar powder was added to Comparative Example 1 and Example 1. In Comparative Example 1, A shows the state of the culture medium when tryptic soy agar powder was added, and B shows the state of the culture medium when tryptic soy agar powder was added to Example 1. Figure 3 The images show the solidified state of the agar medium prepared in Examples 1 and 2. In Example 1, A shows the solidified state of the tryptic soy peptone agar medium prepared in Example 1, and B shows the solidified state of the Sabouraud dextrose agar medium prepared in Example 2. Detailed Implementation

[0015] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0016] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0017] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0018] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0019] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0020] The tryptic soy peptone agar medium and Sabouraud dextrose agar medium used in this invention are produced by Beijing Sanyao Technology Development Co., Ltd. and purchased through Shaanxi Standard & Poor Medicine Technology Co., Ltd.

[0021] Example 1 Heat 1L of purified water until bubbling appears at the bottom (lower layer temperature 90℃, middle layer temperature 88℃, upper layer temperature 86℃). Add 40.0g of tryptic soy agar powder at a rate of 1000g / min, stir rapidly for 3min until no lumps remain, let stand for 3min until completely dissolved, dispense into containers, and autoclave at 121℃ for 15min. Figure 1 Image A shows a photograph of dry powder of tryptic soy peptone agar medium. Figure 2 As shown in Figure B, this is a photograph of the culture medium's state after adding tryptic soy peptone agar powder. Figure 3 Figure A shows the state of the prepared tryptic soy agar medium after solidification. The density of the dissolved and homogenized tryptic soy agar medium is 1060 kg / m³. 3 .

[0022] Example 2 Heat 1L of purified water until bubbling appears at the bottom (lower layer temperature 90℃, middle layer temperature 88℃, upper layer temperature 86℃). Add 40.0g of Sabouraud dextrose agar powder at a rate of 1000g / min, stir rapidly for 3min until no lumps remain, let stand for 3min until completely dissolved, dispense into containers, and autoclave at 121℃ for 15min. Figure 1 Image B shows a photograph of Sabouraud dextrose agar medium dry powder, as shown below. Figure 3 Figure B shows the state of the prepared Sabouraud dextrose agar medium after solidification.

[0023] Comparative Example 1 Heat 1L of purified water (lower layer temperature 100℃, middle layer temperature 95℃, upper layer temperature 90℃), add 40.0g of tryptic soy agar powder at a rate of 1500g / min, stir rapidly for 30min, let stand for 3min until completely dissolved, dispense into containers, and autoclave at 121℃ for 15min. Figure 2 Image A shows the state of the culture medium after adding tryptic soy agar powder. The density of the tryptic soy agar medium after thorough dissolution is 1180 kg / m³. 3 .

[0024] In summary, this invention proposes a novel method for preparing agar medium, which solves the problems of agar medium easily clumping and being difficult to dissolve when exposed to hot water, and achieves the technical effects of shortening the heating and dissolving time of agar medium and making the agar medium dissolve more uniformly.

[0025] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A novel method for preparing agar culture medium, characterized in that, Includes the following steps: Heat purified water until it bubbles at the bottom, add agar medium powder, stir quickly until there are no lumps, and let it stand until completely dissolved.

2. The novel preparation method according to claim 1, characterized in that, The purified water is heated to the point where bubbles appear at the bottom, specifically with the following temperature ranges: the lower layer water temperature is 90℃, the middle layer water temperature is 88℃, and the upper layer water temperature is 86℃.

3. The novel preparation method according to claim 1, characterized in that, The ratio of agar medium powder to purified water is 5g:77~125ml.

4. The novel preparation method according to claim 1, characterized in that, The agar medium powder was added at a rate of 1000 g / min.

5. The novel preparation method according to claim 1, characterized in that, The rapid stirring time is 2-3 minutes.

6. The novel preparation method according to claim 1, characterized in that, The settling time is 2-3 minutes.

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