Weeding composition, preparation method and application
By combining dichloroisoxane with clodinafop-propargyl or oxazolidinafop-propargyl, the problem of herbicide resistance to acetyl-CoA carboxylase inhibitors has been solved, achieving efficient control and improved safety against weeds such as barnyard grass in rice paddies and alopecuroides in wheat fields.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-13
- Publication Date
- 2026-04-17
AI Technical Summary
Existing acetyl-CoA carboxylase (ACCase) inhibitor herbicides have led to resistance problems due to long-term use, making them ineffective in controlling weeds such as barnyard grass in rice fields and wheatgrass in wheat fields. Furthermore, the dosage of dichlorvos isoxaflutole is high, posing a safety risk.
Dichloroisoxane is compounded with cyclophosphamide or oxazolidinone in a specific ratio to form a herbicidal composition, which is used for spraying to control grass and broadleaf weeds.
It achieves broad-spectrum killing effects on grass and broadleaf weeds, reduces the dosage of oxychlorpyrifos or oxadiazon, improves safety for wheat and rice, and solves the problem of controlling intractable weeds.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of agricultural technology, and in particular relates to a weed control composition, its preparation method, and its application. Background Technology
[0002] Field weeds are a major biological disaster affecting the yield and quality of key crops such as rice and wheat. With the promotion of simplified cultivation methods such as large-scale and mechanized farming, and the long-term use of herbicides, the damage caused by weeds in farmland continues to worsen, affecting vast areas. Rice paddy weeds mainly include barnyard grass, Echinochloa crus-galli, and other grasses, as well as various broadleaf weeds and sedges; wheat paddy weeds mainly include Alopecurus aequalis, Alopecurus japonicus, and Gynostemma pentaphyllum, as well as broadleaf weeds such as Galium aparine and Desmodium styracifolium. These weeds compete with crops for resources, seriously threatening agricultural production safety.
[0003] Acetyl-CoA carboxylase (ACCase) inhibitors are key herbicides for controlling grassy weeds, killing them by inhibiting fatty acid synthesis. Commercially available ACCase inhibitor herbicides worldwide mainly fall into three categories: aryloxyphenoxypropionates (FOP), cyclohexene derivatives (CHD), and phenylpyrazoline derivatives (PPZ). However, their long-term continuous use has led to a severe resistance problem. ACCase inhibitor-resistant populations have been found in wheat fields (such as *Alopecurus aequalis* and *Barnyard grass*) and rice paddies in many regions. Resistance forces farmers to increase application rates, creating a vicious cycle of "increased application - intensified resistance," which not only shortens the lifespan of the herbicides but also exacerbates environmental and agricultural safety risks.
[0004] Isoxaben is a selective herbicide with a unique mechanism of action, acting as a deoxy-D-xyulose phosphate synthase (DOXP) inhibitor. It is primarily absorbed through plant roots and seedlings, translocated upwards, and inhibits carotenoid synthesis, disrupting weed photosynthesis and causing chlorosis and death. It is characterized by relatively good safety and a long residual effect, and is mainly used for controlling broadleaf weeds; however, in actual production, the application dosage is often high. Summary of the Invention
[0005] Purpose of the invention: The purpose of this invention is to provide a herbicidal composition with a broad spectrum of weed control, which can effectively cover common weeds in the field and has high safety; the second purpose is to provide a method for preparing the herbicidal composition and its application.
[0006] Technical solution: The herbicidal composition of the present invention includes dichloroisoxane, and any one of cyclophosphamide and oxazolidinyl.
[0007] Preferably, the composition is dichloroisoxane and cyclohexane, with a weight ratio of 1:0.13~2.23.
[0008] More preferably, the weight ratio of dichloroisoxane to cyclophosphamide is 1:0.13~1.12.
[0009] Most preferably, the weight ratio of dichloroisoxane to cyclophosphamide is 1:0.27~1.12.
[0010] Preferably, the composition is dichloroisoxane and oxazolidinyl acetonide in a weight ratio of 1:0.27~4.45.
[0011] More preferably, the weight ratio of dichloroisoxane to oxazolidinium is 1:0.55 ~ 2.23.
[0012] The method for preparing the herbicidal composition of the present invention includes the following steps: mixing dichloroisoxane with any one of cyclophosphamide and oxazolidinyl to obtain the herbicidal composition.
[0013] The application of the herbicidal composition of the present invention in crop cultivation.
[0014] Preferably, the application is used for weed control in crop cultivation.
[0015] Preferably, the crop is rice or wheat.
[0016] Preferably, the weeds are grassy weeds and / or broadleaf weeds.
[0017] More preferably, the weeds include any one or more of the following: barnyard grass, large-spike barnyard grass, Japanese barnyard grass, multiflora ryegrass, barnyard grass, barnyard grass, crabgrass, chickweed, shepherd's purse, and shepherd's purse, as well as other common and difficult-to-control weeds in wheat fields and rice fields.
[0018] Preferably, when the herbicidal composition is dichloroisoxane and clodinafop-propargyl, the dosage range of dichloroisoxane is 13.5~54 g ai / hm. 2 The dosage range of clopyralid is 7.5~30 g ai / hm. 2 Further preferably, the dosage range of dichloroisoxane is 27~54 g ai / hm. 2 The dosage range of clopyralid is 7.5~30 g ai / hm. 2 Most preferably, the dosage range of dichloroisoxane is 27–54 g ai / hm. 2 The dosage range for clopyralid is 15-30 g a.i. / hm. 2 .
[0019] Preferably, when the herbicidal composition is dichloroisoxazone and oxazolidinone, the dosage range of dichloroisoxazone is 13.5~54 g ai / hm.2 The dosage range of oxazolidinone is 15~60 g ai / hm. 2 Further preferably, the dosage range of dichloroisoxane is 27~54 g ai / hm. 2 The dosage range of oxazolidinone is 30~60 ga.i. / hm. 2 .
[0020] Preferably, the application process involves spraying the herbicidal composition onto wheat and rice fields to control weeds.
[0021] Beneficial Effects: Compared with the prior art, the present invention has the following significant advantages: 1. The two herbicides in this herbicidal composition have complementary weed-killing spectra, which can effectively kill different groups of weeds and effectively cover common field weeds such as grasses and broadleaf weeds; 2. The two herbicides in this herbicidal composition have a significant synergistic effect when used in combination, which can greatly reduce the dosage of oxychlorpyrifos or oxadiazon and improve the safety for wheat and rice; 3. This herbicidal composition can be used to control noxious weeds that are currently difficult to control in wheat and rice fields, relieving the urgent need for pesticides to control difficult-to-control weeds, and has excellent potential for industrial application. Detailed Implementation
[0022] The technical solution of the present invention will be further described below.
[0023] Unless otherwise specified, all materials and reagents used in the following examples are commercially available. Experimental methods not specifically described in the examples are generally performed under standard conditions or as recommended by the manufacturer.
[0024] In this invention, the germplasm materials of *Hemiberlesia lataniae*, *Alopecurus aequalis*, *Lernaea rubra*, *Barnyardgrass*, *Echinochloa crus-galli*, *Digitaria sanguinalis*, wheat (Yangmai 23, Jimai 22), and rice (Nanjing 9108, Yongyou 2640) were all preserved and provided by the laboratory of the College of Agriculture of Yangzhou University.
[0025] Example 1: Formulation screening of dichloroisoxane·clopyralid compound herbicidal composition To screen the optimal formulation ratio of dichloroisoxane and clodinafop-propargyl, a series of whole-plant bioassays were conducted in an artificial climate chamber.
[0026] 1. Test weeds The representative noxious grass weeds that cause serious damage to wheat fields, namely, *Lysimachia christinae*, *Alopecurus aequalis*, and *Lolium perfringens*, were selected as the test weeds.
[0027] 2. Screening test of formulation ratio of dichloroisoxane·clopyralid compound herbicides and dosage setting of pesticides. (1) The dosage settings for each single dose are as follows: Dichloroisoxane (A): 0 (A0), 13.5 (A13.5), 27 (A27), 54 (A54) g ai / hm 2 ; Phosphatidylcholine (B): 0 (B0), 7.5 (B7.5), 15 (B15), 30 (B30) g ai / hm 2 .
[0028] (2) The formulation ratio screening dosage settings are shown in Table 1: Table 1. Screening and Dosage Setting Table for Herbicidal Composition Formulations
[0029] A0B0 is the control group.
[0030] 3. Test methods Fill square plastic plant cultivation pots (7×7×7 cm) with organic soil that has not been treated with herbicides (pH 6.5, organic matter content 2.4%), and add water until saturated. Select plump seeds of *Lysimachia christinae*, *Alopecurus aequalis*, and *Lolium multiflorum*, and sow 20 seeds in each pot. Cover the seeds with a thin layer of fine soil (about 3 mm thick). Before sowing, rub the *Lysimachia christinae* seeds in the palms of your hands to break the air sacs attached to the seeds and promote germination. Place the pots in a 44×33×10 cm thickened plastic transfer box (pre-filled with 2 cm of water) and place it in an artificial climate chamber for cultivation.
[0031] The cultivation conditions were set as follows: 12 h of light at 15℃ and 12 h of darkness at 10℃. During the experiment, a water layer of 0.5 cm to 1 cm was maintained in the transfer box to keep the soil in the small boxes moist through water absorption. Each treatment was replicated in 4 small pots. When the weed seedlings in the test pots reached the 3-leaf stage, they were thinned to 10 plants per small pot. When the weed seedlings reached the 4-leaf stage, herbicides were applied by spraying, while the control group was sprayed with an equal amount of water. Spraying was carried out using a mobile pressure-stabilized spray tower with a spray height of 20 cm, a spray width of 50 cm using a flat fan-shaped nozzle, and a pressure of 200 kPa. The spray solution volume corresponded to a field spray volume of 45 L water / hm². 2 Thirty days after application, the fresh weight of the upper part of the tested weeds in each small flowerpot was collected and weighed.
[0032] Calculate the measured fresh weight inhibition rate (E) and theoretical fresh weight inhibition rate (E0) of weeds among different treatments of single-agent and compound herbicides. The Gowing method is used to evaluate the synergistic effect type of binary compound herbicides by comparing E and E0 values; the calculation formula is as follows: E = (Fresh weight of control group – Fresh weight of treatment group) ÷ Fresh weight of control group × 100%; E0 = X + Y – X × Y × 100; Where X is the measured fresh weight inhibition rate of dichlorvos alone, and Y is the measured fresh weight inhibition rate of clodinafop-propargyl alone.
[0033] Using E-E0 as the combined effect value, when the combined effect value is >10%, it indicates that the two herbicides have a synergistic effect; when the combined effect value is <-10%, it indicates that the two herbicides have an antagonistic effect; when the combined effect value is between -10% and 10%, it is an additive effect.
[0034] 4. Test Results (1) The combined effect of dichloroisoxane·clopyralid compound herbicidal composition on purslane Table 2. Measured fresh weight inhibition rate E (%) of *Bletilla striata*
[0035] Table 3. Inhibition rate of theoretical fresh weight of *Bletilla striata* (E0) (%)
[0036] Table 4 Combined Action Values of Dichloroisoxane·Lysimachia-P-ethyl Herbicides against Blanchylis
[0037] Table 2 shows that the single-agent concentration of cyclophosphamide is 30 g ai / hm. 2 Thirty days after treatment with 4-leaf stage seedlings, the fresh weight inhibition rate of *Bretschneidera sinensis* was only 46.89%; dichlorvos alone showed low inhibitory effect on *Bretschneidera sinensis*, with a single agent dosage of 54 g ai / hm². 2 Thirty days after treatment of 4-leaf stage seedlings, the fresh weight inhibition rate of *Bretschneidera sinensis* was only 11.54%, while the inhibition rate of the two herbicides combined at the same dosage reached 84.02%, showing a very significant improvement. The theoretical inhibition rate of the combined use of the two herbicides is shown in Table 3. By comparing the measured and theoretical inhibition rates of different combinations of the two herbicides, the combined effect of the two herbicides on *Bretschneidera sinensis* was further investigated (Table 4). The results showed a significant combined effect between dichlorvos and clodinafop-propargyl, with dichlorvos at 27-54 g ai / hm². 2 With 7.5 ~ 30 g ai / hm of clopyralid 2 Different dosage combinations all showed that the measured inhibition rate exceeded the theoretical inhibition rate by more than 20%, demonstrating a very significant synergistic effect.
[0038] (2) The combined effect of dichloroisoxane·clopyralid compound herbicides on Alopecurus aequalis indica Table 5. Measured fresh weight inhibition rate E (%) of *Alopecurus aequalis*.
[0039] Table 6. Theoretical fresh weight inhibition rate E0 (%) of *Alopecurus aequalis* (large spikelet)
[0040] Table 7 Combined Effect Values of Dichloroisoxane·Lysimachia-Pyrazosulfuron-methyl Herbicides on Alopecurus aequalis indica
[0041] Table 5 shows that the single-agent concentration of cyclophosphamide is 30 g ai / hm. 2 Thirty days after treatment of 4-leaf stage seedlings, the fresh weight inhibition rate of *Alopecurus aequalis* was only 42.58%; dichlorvos alone showed poor inhibitory effect on *Alopecurus aequalis*, with a single application rate of 54 g a.i. / hm². 2 The fresh weight inhibition rate of the herbicide after treatment of 4-leaf stage seedlings for 30 days was only 9.45%, while the inhibition rate of the two herbicides combined at the same dosage reached 78.26%, showing a very significant improvement effect. The theoretical inhibition rate of the two herbicides combined is shown in Table 6. By comparing the measured and theoretical inhibition rates of the two herbicides under different combinations, the combined effect of the two herbicides on *Alopecurus aequalis* was further investigated (Table 7). The results showed a significant combined effect between dichlorvos and clodinafop-propargyl, with dichlorvos at 27-54 g ai / hm². 2 With 7.5~30 g ai / hm of clopyralid 2 Different dosage combinations all showed that the measured inhibition rate exceeded the theoretical inhibition rate by more than 20%, demonstrating a very significant synergistic effect.
[0042] (3) The combined effect of dichloroisoxane·clopyralid compound herbicidal composition on ryegrass Table 8. Measured fresh weight inhibition rate E (%) of ryegrass
[0043] Table 9. Theoretical fresh weight inhibition rate E0 (%) of ryegrass
[0044] Table 10 Combined Effect Values of Dichloroisoxane·Lysimachia-methyl Compound Herbicidal Combination on Lernica japonica
[0045] Table 8 shows that the single-agent concentration of dichloroisoxane is 54 g ai / hm. 2 Thirty days after treatment of its 4-leaf stage seedlings, the inhibition rate against Lernaea florida was only 26.28%; clodinafop-propargyl at a single application of 30 g ai / hm 2Thirty days after treatment of 4-leaf stage seedlings, the inhibition rate of fresh weight of *Lycium ruthenicum* was only 33.49%, while the inhibition rate of the two herbicides combined at the same dosage reached 83.65%, showing a significant improvement. The theoretical inhibition rate of the combined use of the two herbicides is shown in Table 9. By comparing the measured and theoretical inhibition rates of the two herbicides under different combinations, the combined effect of the two herbicides on *Lycium ruthenicum* was further investigated (Table 10). The results showed a significant combined effect between dichlorvos and clodinafop-propargyl, with dichlorvos at a concentration of 27–54 g.i. / hm². 2 With 7.5~30 g ai / hm of clopyralid 2 Different dosage combinations all showed that the measured inhibition rate exceeded the theoretical inhibition rate by more than 20%, demonstrating a very significant synergistic effect.
[0046] 5. Experimental Conclusions The above results indicate that the combined use of dichloroisoxane and clodinafop-propargyl has a significant inhibitory effect and synergistic effect on various weeds.
[0047] Further analysis of the ratio of dichlorvos to clodinafop-propargyl in the compound formulation revealed that dichlorvos ranged from 13.5 to 54 ga.i. / hm. 2 With 7.5~30 g ai / hm of clopyralid 2 Different dosage combinations showed synergistic effects. Therefore, the weight ratio of dichloroisoxane to clodinafop-propargyl is: dichloroisoxane: clodinafop-propargyl = 1: 0.13~2.23.
[0048] dichlorvos 27-54 g ai / hm 2 With 7.5~30 g ai / hm of clopyralid 2 The combined effect value (E-E0) of various combinations is above 20%. Therefore, the optimal ratio range of dichloroisoxane and clodinafop-propargyl is: dichloroisoxane: clodinafop-propargyl = 1: 0.13~1.12.
[0049] dichlorvos 27-54 g ai / hm 2 With 15-30 g ai / hm of clopyralid 2 The combined effect value (E-E0) of various combinations is significantly higher than that of other combinations. Therefore, the optimal weight ratio range of dichloroisoxane and clodinafop-propargyl is: dichloroisoxane: clodinafop-propargyl = 1: 0.27~1.12.
[0050] Example 2: Safety determination of dichloroisoxane·clopyralid compound herbicide composition on wheat To determine the safety of the dichloroisoxane·clopyralid compound herbicidal composition for wheat, a series of whole-plant bioassays were conducted in an artificial climate chamber.
[0051] 1. Test crop Yangmai 23 and Jimai 22 were used as the test wheat varieties.
[0052] 2. Dosage setting (1) Dichloroisoxane 0 g ai / hm 2 0 g ai / hm of clopyralid 2 (Control group) (2) Dichloroisoxane 108 g ai / hm 2 60 g ai / hm of clopyralid 2 (3) Dichloroisoxane 216 g ai / hm 2 120 g ai / hm of clopyralid 2 3. Test methods Organic soil (pH 6.5, organic matter content 2.4%) that had not been treated with herbicides was placed in plastic boxes measuring 22×15×10 cm. The soil layer in each box was approximately 8 cm high, and holes were punched in the bottom of the plastic boxes to facilitate water absorption. The plastic boxes were placed in a thickened plastic transfer box (44×33×10 cm, pre-filled with 2 cm of clean water) and placed in an artificial climate chamber for cultivation. During the experiment, a water layer of approximately 0.5 cm was maintained in the transfer box to keep the soil in the plastic boxes moist through water absorption. Each treatment was replicated with 4 plastic boxes. Wheat seedling cultivation conditions were set as follows: 15℃ light for 12 h, 10℃ darkness for 12 h. Spraying treatments were applied at the 4-leaf stage of wheat, while the control group was sprayed with an equal amount of clean water. Spraying was carried out using a row-mounted pressure-controlled spray tower with a spray height of 20 cm, a spray width of 50 cm using a flat fan-shaped nozzle, and a pressure of 200 kPa. The spray volume corresponded to a field spray volume of 45 L water / hm2 Thirty days after application, the fresh weight of the aboveground wheat parts in each plastic box was collected and weighed. The inhibition rate E of different treatments on the fresh weight of the aboveground wheat parts was calculated using the following formula: E = (Fresh weight of control group - Fresh weight of treatment group) ÷ Fresh weight of control group × 100%.
[0053] 4. Test Results Table 11 Inhibition rate (E) of the dichloroisoxane·clopyralid compound herbicide composition on the aboveground fresh weight of wheat (%)
[0054] 5. Experimental Conclusions As shown in Table 11, dichlorvos·clopyralid was compounded at a ratio of 1:0.56 at a concentration of 168~336 g ai / hm. 2 The treatment still provides good safety for wheat.
[0055] Example 3: Formulation screening of dichlorvos·oxazolidinone compound herbicidal composition To screen the optimal formulation ratio of dichloroisoxane and oxazolidinyl, a series of whole-plant bioassays were conducted in an artificial climate chamber.
[0056] 1. Test weeds The representative noxious grass weeds that cause serious damage to paddy fields, namely barnyard grass, Echinochloa crus-galli, and Crataegus pinnatifida, were selected as test weeds.
[0057] 2. Screening test of formulation ratio and dosage setting of dichloroisoxane·oxazolidinyl chlorpyrifos compound herbicide composition (1) The dosage settings for each single dose are as follows: Dichloroisoxane (A): 0 (A0), 13.5 (A13.5), 27 (A27), 54 (A54) g ai / hm 2 ; Oxazolidinone (B): 0 (B0), 15 (B15), 30 (B30), 60 (B60) g ai / hm 2 .
[0058] (2) The formulation ratio screening dosage settings are shown in Table 12: Table 12 Screening and Dosage Setting Table for Herbicidal Composition Formulation
[0059] A0B0 is the control group.
[0060] 3. Test methods Fill square plastic plant cultivation pots (7×7×7 cm) with organic soil that has not been treated with herbicides (pH 6.5, organic matter content 2.4%) and add water until saturated. Select plump barnyard grass, Echinochloa crus-galli, and Digitaria sanguinalis seeds, sow 20 seeds in each small pot, cover the seeds with a layer of fine soil (about 3 mm thick), place the small pots in a 44×33×10 cm thickened plastic transfer box (pre-filled with 2 cm of clean water), and place them in an artificial climate chamber for cultivation.
[0061] The cultivation conditions were set as follows: 12 h of light at 15℃ and 12 h of darkness at 10℃. During the experiment, a water layer of 0.5 cm to 1 cm was maintained in the transfer box to keep the soil in the small boxes moist through water absorption. Each treatment was replicated in 4 small pots. When the weed seedlings in the test pots reached the 3-leaf stage, they were thinned to 10 plants per small pot. When the weed seedlings reached the 4-leaf stage, herbicides were applied by spraying, while the control group was sprayed with an equal amount of water. Spraying was carried out using a mobile pressure-stabilized spray tower with a spray height of 20 cm, a spray width of 50 cm using a flat fan-shaped nozzle, and a pressure of 200 kPa. The spray solution volume corresponded to a field spray volume of 45 L water / hm². 2 Thirty days after application, the fresh weight of the upper part of the tested weeds in each small flowerpot was collected and weighed.
[0062] Calculate the measured fresh weight inhibition rate (E) and theoretical fresh weight inhibition rate (E0) of weeds among different treatments of single-agent and compound herbicides. The Gowing method is used to evaluate the synergistic effect type of binary compound herbicides by comparing E and E0 values; the calculation formula is as follows: E = (Fresh weight of control group – Fresh weight of treatment group) ÷ Fresh weight of control group × 100%; E0 = X + Z – X × Z × 100; Where X is the measured fresh weight inhibition rate of dichlorvos alone, and Z is the measured fresh weight inhibition rate of oxazolidinone alone.
[0063] Using E-E0 as the combined effect value, when the combined effect value is >10%, it indicates that the two herbicides have a synergistic effect; when the combined effect value is <-10%, it indicates that the two herbicides have an antagonistic effect; when the combined effect value is between -10% and 10%, it is an additive effect.
[0064] 4. Test Results (1) The combined effect of dichlorvos·oxazolidinone and oxazolidinone compound herbicides on barnyardgrass Table 13. Measured fresh weight inhibition rate of barnyard grass E (%)
[0065] Table 14. Inhibition rate of barnyardgrass theoretical fresh weight E0 (%)
[0066] Table 15 Combined Action Values of Dichloroisoxane·Oxazoline Compound Herbicidal Combination Against Barnyardgrass
[0067] Table 13 shows that dichlorvos alone has a low inhibitory effect on barnyardgrass; dichlorvos alone has a concentration of 54 g.i. / hm. 2Thirty days after treatment of 4-leaf stage seedlings, the fresh weight inhibition rate of barnyardgrass was only 4.71%, while that of oxazolidinone at 60g ai / hm was significantly higher. 2 Thirty days after treatment of 4-leaf stage seedlings, the fresh weight inhibition rate of the tested barnyardgrass was only 45.15%, while the inhibition rate of the two herbicides combined at the same dosage reached 83.80%, showing a very significant improvement. The theoretical inhibition rate of the combined use of the two herbicides is calculated and shown in Table 14. By comparing the measured and theoretical inhibition rates of the two herbicides under different combinations, the combined effect of the two herbicides on barnyardgrass was further investigated (Table 15). The results showed a significant combined effect between dichlorvos and oxadiazon, with dichlorvos at dosages ranging from 13.5 to 54 g ai / hm². 2 With oxazolidinone 15~60 g ai / hm 2 Different dosage combinations all showed that the measured inhibition rate exceeded the theoretical inhibition rate by more than 20%, indicating a synergistic effect.
[0068] (2) The combined effect of dichloroisoxane·oxazolidinyl acetamiprid compound herbicide composition on Echinochloa crus-galli. Table 16. Measured fresh weight inhibition rate E (%) of Echinopsis thunbergii
[0069] Table 17. Theoretical fresh weight inhibition rate E0 (%) of *Euphorbia lathyris*
[0070] Table 18 Combined Effect Values of Dichloroisoxane·Oxazoline Compound Herbicidal Combination on Echinochloa crus-galli
[0071] Table 16 shows that the single-agent concentration of dichlorvos is 54 g ai / hm. 2 Thirty days after treatment of its 4-leaf stage seedlings, the inhibition rate against *Echinochloa crus-galli* was only 14.10%; oxazolidinone at a concentration of 60 g ai / hm² was also effective. 2 Thirty days after treatment of 4-leaf stage seedlings, the fresh weight inhibition rate of *Echinochloa crus-galli* was only 30.74%, while the inhibition rate of the two herbicides combined at the same dosage reached 73.78%, showing a significant improvement. The theoretical inhibition rate of the combined use of the two herbicides is calculated and shown in Table 17. By comparing the measured and theoretical inhibition rates of different combinations of the two herbicides, the combined effect of the two herbicides on *Echinochloa crus-galli* was further investigated (Table 18). The results showed a significant combined effect between dichlorvos and oxadiazon, with dichlorvos at a dosage of 13.5–54 g.i. / hm². 2 With oxazolidinone 15~60 g ai / hm 2Different dosage combinations all showed that the measured inhibition rate exceeded the theoretical inhibition rate by more than 20%, indicating a synergistic effect.
[0072] (3) The combined effect of dichlorvos·oxazolidinone compound herbicide composition on barnyardgrass Table 19 Measured Fresh Weight Inhibition Rate E (%) of Crataegus pinnatifida
[0073] Table 20. Theoretical fresh weight inhibition rate of Digitaria sanguinalis, E0 (%)
[0074] Table 21 Combined Effect Values of Dichloroisoxane·Oxazoline Compound Herbicidal Combination on Digitaria sanguinalis
[0075] Table 19 shows that diclofenac monotherapy has poor inhibitory effect on barnyardgrass, with a concentration of 54 ga.i. / hm. 2 Thirty days after treatment of its 4-leaf stage seedlings, the inhibition rate against barnyardgrass was only 7.92%; oxazolidinone at a concentration of 60 g ai / hm 2 Thirty days after treatment of 4-leaf stage seedlings, the fresh weight inhibition rate of barnyardgrass was 41.29%, while the inhibition rate of the two herbicides combined at the same dosage reached 83.38%, showing a very significant improvement. The theoretical inhibition rate of the two herbicides combined is shown in Table 20. By comparing the measured and theoretical inhibition rates of the two herbicides under different combinations, the combined effect of the two herbicides on barnyardgrass was further investigated (Table 21). The results showed a significant combined effect between dichlorvos and oxadiazon, with dichlorvos at dosages of 13.5–54 g ai / hm². 2 With oxazolidinone 15~60 g ai / hm 2 Different dosage combinations all showed that the measured inhibition rate exceeded the theoretical inhibition rate by more than 20%, indicating a synergistic effect.
[0076] 5. Experimental Conclusions The above results indicate that the combined use of dichlorvos and oxazolidinone has a significant inhibitory effect and synergistic effect on various weeds.
[0077] Further analysis of the ratio of dichloroisoxane to oxazolidinyl in the compound formulation revealed that dichloroisoxane was 13.5–54 g a.i. / hm. 2 With oxazolidinone 15~60 g ai / hm 2Different combinations of dosages showed synergistic effects. Therefore, the ratio of dichloroisoxane to oxazolidinium is: dichloroisoxane: oxazolidinium = 1: 0.27~4.45.
[0078] dichlorvos 27-54 g ai / hm 2 With oxazolidinone 30~60 g ai / hm 2 The combined effect value (E-E0) of various combinations is above 30%. Therefore, the optimal ratio range of dichloroisoxane and oxazolidinone is: dichloroisoxane: oxazolidinone = 1: 0.55~2.23.
[0079] Example 4: Safety test of dichlorvos·oxazolidinone compound herbicide composition on rice To determine the safety of the dichlorvos-oxazolidinone-oxazolidinone combination herbicide composition for rice, a series of whole-plant bioassays were conducted in an artificial climate chamber.
[0080] 1. Test crop Nanjing 9108 was the tested japonica rice variety, and Yongyou 2640 was the tested indica rice variety.
[0081] 2. Dosage setting (1) Dichloroisoxane 0 g ai / hm 2 Oxazolidinone 0 g ai / hm 2 (Control group) (2) Dichloroisoxane 54 g ai / hm 2 Oxazolidinone 60 g ai / hm 2 (3) Dichloroisoxane 108 g ai / hm 2 Oxazolidinone 120 g ai / hm 2 (4) Dichloroisoxane 216 g ai / hm 2 Oxazolidinone 240 g ai / hm 2 3. Test methods In plastic boxes measuring 22×15×10 cm, untreated organic soil (pH 6.5, organic matter content 2.4%) was filled, with a soil layer height of approximately 8 cm in each box. The bottom of the plastic boxes was perforated for water absorption. These boxes were then placed in a 44×33×10 cm thickened plastic transfer box (pre-filled with 2 cm of water) and cultured in an artificial climate chamber. Rice seedling culture conditions were set as follows: 30℃ light for 12 h, 20℃ darkness for 12 h. Each treatment was replicated with four plastic boxes, and spraying was performed at the 4-leaf stage of rice. The control group was sprayed with an equal amount of water. Spraying was conducted using a row-mounted pressure-controlled spray tower with a spray height of 20 cm, a spray width of 50 cm using a flat fan-shaped nozzle, and a pressure of 200 kPa. The spray volume corresponded to a field spray volume of 45 L water / hm2 Thirty days after application, the fresh weight of the aboveground parts of the tested rice plants was collected and weighed from each plastic box. The inhibition rate E of different treatments on the fresh weight of the aboveground parts of rice was calculated using the following formula: E = (Fresh weight of control group - Fresh weight of treatment group) ÷ Fresh weight of control group × 100%.
[0082] 4. Test Results Table 22 Inhibition rate (E) of the dichloroisoxane·oxazolidinyl compound herbicide composition on the aboveground fresh weight of rice (%)
[0083] 5. Experimental Conclusions As shown in Table 22, dichlorvos·oxazolidinone was compounded at a ratio of 1:1.11 at a concentration of 114~456 g.i. / hm. 2 The treatment still provides good safety for rice.
Claims
1. A herbicidal composition, characterized by, The composition includes dichloroisoxane, and either cyclophosphamide or oxazolidinone.
2. The herbicidal composition according to claim 1, characterized by The composition is dichloroisoxane and cyclohexane, in a weight ratio of 1:0.13~2.
23.
3. The herbicidal composition according to claim 1, characterized in that, The composition is dichloroisoxane and oxazolidinone in a weight ratio of 1:0.27~4.
45.
4. A method for preparing the herbicidal composition according to claim 1, characterized in that, The process includes the following steps: mixing dichloroisoxane with either cyclophosphamide or oxazolidinyl to obtain a herbicidal composition.
5. The use of the herbicidal composition of claim 1 in crop cultivation.
6. The application according to claim 5, characterized in that, The application described is for weed control in crop cultivation.
7. The application according to claim 6, characterized in that, The crop in question is either rice or wheat.
8. The application according to claim 6, characterized in that, The weeds are grassy weeds and / or broadleaf weeds.
9. The application according to claim 8, characterized in that, The weeds include any one or more of the following: barnyard grass, large-spike barnyard grass, Japanese barnyard grass, multiflora ryegrass, barnyard grass, barnyard grass, crabgrass, chickweed, shepherd's purse, and shepherd's purse.
10. The application according to claim 5, characterized in that, when the herbicidal composition is dichloroisoniximic ketone and pinoxaden, the dosage range of dichloroisoniximic ketone is 13.5 ~ 54 g a.i. / hm 2 , the dosage range of pinoxaden is 7.5 ~ 30 g a.i. / hm 2 ; when the herbicidal composition is dichloroisoniximic ketone and metamifop, the dosage range of dichloroisoniximic ketone is 13.5 ~ 54 g a.i. / hm 2 , the dosage range of metamifop is 15 ~ 60 g a.i. / hm 2 .