Effective composition for reducing total sugar content of tobacco leaves and increasing potassium content of tobacco leaves and application thereof

Abstract

The invention relates to a functional composition for reducing the total sugar content of tobacco leaves and increasing the potassium content of the tobacco leaves and application of the functional composition. The functional composition comprises a first component, a second component and a third component, the first component comprises potassium hydrogen persulfate; the second component comprises trisodium phosphate; and the third component comprises glucose oxidase. The raw materials including the potassium hydrogen persulfate, the trisodium phosphate and the glucose oxidase are combined for use and are matched with one another, the effects of reducing the total sugar content in the tobacco leaves and increasing the potassium content in the tobacco leaves can be achieved after the composition is applied in the tobacco growth period, then the sugar-alkali ratio and the sugar-nitrogen ratio of the tobacco leaves are balanced, the sweet sense and the aroma amount of the tobacco leaves are increased, and the tobacco quality is improved. The comfort of the sensory smoking quality of the tobacco leaves is improved.

Description

Technical Field

[0001] This invention belongs to the field of tobacco planting technology, and relates to an effective composition for reducing the total sugar content and increasing the potassium content of tobacco leaves, and its application. Background Technology

[0002] Tobacco primarily provides consumers with pleasure and satisfaction through combustion and inhalation. The sugar and potassium content in tobacco leaves is an important characteristic of the inherent aroma quality of tobacco leaves and is closely related to the sensory evaluation of tobacco quality.

[0003] Yunnan tobacco leaves are renowned for their high sugar content and prominent sweet aroma, which is a crucial material basis for the inherent sweet and pleasant aroma of local tobacco leaves and for enhancing their overall aroma intensity. However, a higher total sugar content is not always better; it is generally advisable to maintain a sugar content of 25-36%, and not exceeding 36%, to achieve a balance in the sugar-to-alkaloid and sugar-to-nitrogen ratios, thereby improving the sweetness and comfort of the tobacco. On the other hand, a potassium content of 1.3% or higher is generally considered ideal for tobacco leaves, as it can enhance their aroma intensity. However, in many tobacco-growing areas of Yunnan, the potassium content of tobacco leaves does not exceed 1.3%. Applying potassium sulfate not only increases costs but also has limited effect on increasing the potassium content, making it difficult to improve the aroma intensity. Furthermore, under the same ecological environment and the same variety conditions, the total nitrogen and nicotine content of tobacco leaves remain almost constant. Therefore, the lower the total sugar content and the higher the potassium content, the better the aroma intensity, sweetness, and comfort of the tobacco leaves.

[0004] The persistently high total sugar content and difficulty in increasing potassium ion content in Yunnan tobacco leaves have long constrained the further improvement and development of Yunnan tobacco quality, even leading to a decline in the sweet and aromatic characteristics of local tobacco, as well as a weakening of aroma quantity and sweetness. Currently, the total sugar content of Yunnan tobacco leaves typically exceeds 36%, and even exceeds 40%, with no effective measures to reduce sugar content. At the same time, the potassium content (potassium oxide) of tobacco leaves in most producing areas is usually between 1.7% and 2.0%, making further increases difficult.

[0005] Therefore, it would be very meaningful to develop a strategy that can reduce the total sugar content and increase the potassium content of tobacco leaves in order to enhance the sweet and fragrant style of tobacco leaves, increase the sweetness and aroma of tobacco leaves, and thus improve consumers' sensory evaluation of the quality of tobacco leaves. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the purpose of this invention is to provide an effective composition for reducing the total sugar content and increasing the potassium content of tobacco leaves, and its application.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides an effective composition for reducing the total sugar content and increasing the potassium content of tobacco leaves, the effective composition comprising a first component, a second component and a third component;

[0009] The first component includes potassium persulfate; the second component includes trisodium phosphate; and the third component includes glucose oxidase.

[0010] This invention creatively develops an efficacy composition that combines raw materials including potassium persulfate, trisodium phosphate, and glucose oxidase. The three work together to reduce the total sugar content and increase the potassium content in tobacco leaves when applied during the main growth period of tobacco. This balances the sugar-alkali ratio and sugar-nitrogen ratio of tobacco leaves, improves the sweetness and aroma of tobacco leaves, and enhances the sensory evaluation of the comfort of smoking tobacco leaves.

[0011] Preferably, the mass ratio of the first component, the second component, and the third component is (2-5):(1-3):(0.25-1).

[0012] The specific point values ​​in (2-5) can be 2, 2.5, 3, 3.5, 4, 4.5, 5, etc.; the specific point values ​​in (1-3) can be 1, 1.5, 2, 2.5, 3, etc.; the specific point values ​​in (0.25-1) can be 0.25, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, etc.; other specific point values ​​not listed in this range can be selected, and will not be elaborated here.

[0013] Based on the synergistic relationship between the first, second, and third components, when they meet the specific mass ratios mentioned above, they are more effective in reducing the total sugar content and increasing the potassium content in tobacco leaves.

[0014] Preferably, the first component further includes any one or a combination of at least two of potassium bisulfate, potassium sulfate, potassium peroxymonosulfate, or calcium peroxide.

[0015] In this invention, potassium peroxymonosulfate in the first component can be partially replaced by any one or a combination of at least two of potassium bisulfate, potassium sulfate, potassium peroxymonosulfate, or calcium peroxide. More preferably, potassium peroxymonosulfate and calcium peroxide are used simultaneously, which is more effective than potassium peroxymonosulfate alone. That is, calcium peroxide plays an important role in assisting potassium peroxymonosulfate in reducing the total sugar content and increasing the potassium content in tobacco leaves.

[0016] Preferably, the first component comprises a combination of potassium persulfate and calcium peroxide.

[0017] Preferably, the mass ratio of potassium persulfate to calcium peroxide is (1-5):1, such as 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, 4.5:1, 5:1, etc. Other specific values ​​not listed within this range can be selected, and will not be elaborated here.

[0018] Preferably, the second component further includes any one or a combination of at least two of disodium hydrogen phosphate, sodium hexametaphosphate, or sodium hypophosphite.

[0019] In this invention, the trisodium phosphate in the second component can be partially replaced by any one or a combination of at least two of disodium hydrogen phosphate, sodium hexametaphosphate, or sodium hypophosphite. More preferably, trisodium phosphate, sodium hexametaphosphate, and sodium hypophosphite are used simultaneously, which is more effective than trisodium phosphate alone. That is, sodium hexametaphosphate and sodium hypophosphite play an important role in assisting trisodium phosphate in reducing the total sugar content and increasing the potassium content in tobacco leaves.

[0020] Preferably, the second component comprises a combination of trisodium phosphate, sodium hexametaphosphate, and sodium hypophosphite.

[0021] Preferably, the mass ratio of trisodium phosphate, sodium hexametaphosphate and sodium hypophosphite is (3-5):(1-2):1.

[0022] The specific point values ​​in (3-5) can be 3, 3.5, 4, 4.5, 5, etc.; the specific point values ​​in (1-2) can be 1, 1.2, 1.5, 1.8, 2, etc.; other specific point values ​​not listed in this range can be selected, and will not be elaborated here.

[0023] In a second aspect, the present invention provides the use of the efficacy composition according to the first aspect in tobacco cultivation.

[0024] Thirdly, the present invention provides the use of the efficacy composition according to the first aspect in reducing the total sugar content of tobacco leaves and increasing the potassium content of tobacco leaves.

[0025] Fourthly, the present invention provides the use of the efficacy composition according to the first aspect in reducing the sugar-nitrogen ratio and the sugar-alkali ratio of tobacco leaves.

[0026] Fifthly, the present invention provides a cultivation method for reducing the total sugar content and increasing the potassium content of tobacco leaves, the cultivation method comprising: after transplanting tobacco seedlings, applying the effective composition described in the first aspect on the basis of normal fertilization.

[0027] Preferably, the application method of the efficacy composition includes: applying the second and third components of the efficacy composition at any time from the day of transplanting to 15 days after transplanting, wherein the total amount of the second component applied is 1-3 kg / mu (e.g., 1 kg / mu, 1.5 kg / mu, 2 kg / mu, 2.5 kg / mu, 3 kg / mu, etc.), and the total amount of the third component applied is 0.25-1 kg / mu (e.g., 0.25 kg / mu, 0.3 kg / mu, 0.4 kg / mu, 0.5 kg / mu, 0.6 kg / mu, 0.7 kg / mu, 0.8 kg / mu, 1 kg / mu, etc.); other specific values ​​not listed within this range can be selected, and will not be elaborated here.

[0028] Apply the first and third components of the efficacy composition at any time within 30 to 40 days after transplanting. The total application amount of the first component is 2-5 kg / mu (e.g., 2 kg / mu, 2.5 kg / mu, 3 kg / mu, 3.5 kg / mu, 4 kg / mu, 4.5 kg / mu, 5 kg / mu, etc.), and the total application amount of the third component is 0.25-1 kg / mu (e.g., 0.25 kg / mu, 0.3 kg / mu, 0.4 kg / mu, 0.5 kg / mu, 0.6 kg / mu, 0.7 kg / mu, 0.8 kg / mu, 1 kg / mu, etc.). Other specific values ​​not listed within this range can be selected, and will not be elaborated here.

[0029] When the efficacy composition involved in this invention is applied using the specific method described above, that is, by selecting a specific application time and a specific application amount, the effect on reducing the total sugar content of tobacco leaves and increasing the potassium content of tobacco leaves is better.

[0030] Preferably, the first, second, and third components of the efficacy composition are all diluted with water before application, and the dilution ratio is independently selected from any value in the range of 300-500 times, such as 300 times, 330 times, 350 times, 380 times, 400 times, 420 times, 450 times, 500 times, etc. Other specific values ​​within this range that are not listed can be selected, and will not be described in detail here.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] This invention creatively develops an efficacy composition that combines raw materials including potassium persulfate, trisodium phosphate, and glucose oxidase. The three work together to reduce the total sugar content and increase the potassium content in tobacco leaves when applied during the tobacco growing season. This balances the sugar-alkali ratio and sugar-nitrogen ratio in the tobacco leaves, improves the sweetness and aroma of the tobacco leaves, and enhances the sensory evaluation quality of the tobacco leaves. Detailed Implementation

[0033] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0034] The glucose oxidase mentioned below is a water-soluble glucose oxidase purchased from Baiyin Sainuo Animal Health Technology Co., Ltd., with an enzyme activity of ≥ 1000 U / g.

[0035] Example 1

[0036] This embodiment provides an efficacy composition comprising a first component, a second component, and a third component in a mass ratio of 3:1.5:0.5. The first component is potassium persulfate, the second component is trisodium phosphate, and the third component is glucose oxidase.

[0037] Example 2

[0038] This embodiment provides an efficacy composition comprising a first component, a second component, and a third component in a mass ratio of 5:1:0.8. The first component is a combination of potassium peroxymonosulfate and potassium peroxymonosulfate in a mass ratio of 3:1; the second component is a combination of trisodium phosphate and disodium hydrogen phosphate in a mass ratio of 2:1; and the third component is glucose oxidase.

[0039] Example 3

[0040] This embodiment provides an efficacy composition comprising a first component, a second component, and a third component in a mass ratio of 2:3:1.2. The first component is a combination of potassium peroxymonosulfate and potassium sulfate in a mass ratio of 4:1; the second component is a combination of trisodium phosphate and disodium hydrogen phosphate in a mass ratio of 1:1; and the third component is glucose oxidase.

[0041] Example 4

[0042] This embodiment provides an efficacy composition comprising a first component, a second component, and a third component in a mass ratio of 4:1:0.5. The first component is a combination of potassium peroxymonosulfate and potassium bisulfate in a mass ratio of 5:1; the second component is a combination of trisodium phosphate and disodium hydrogen phosphate in a mass ratio of 5:1; and the third component is glucose oxidase.

[0043] Example 5

[0044] This embodiment provides an efficacy composition whose components differ from those of Example 1 only in the first component, which consists of a first component, a second component, and a third component in a mass ratio of 3:1.5:0.5. The first component is a combination of potassium persulfate and calcium peroxide in a mass ratio of 2:1, the second component is trisodium phosphate, and the third component is glucose oxidase.

[0045] Example 6

[0046] This embodiment provides an efficacy composition whose components differ from those of Example 1 only in the second component, which consists of a first component, a second component, and a third component in a mass ratio of 3:1.5:0.5. The first component is potassium persulfate, the second component is a combination of trisodium phosphate, sodium hexametaphosphate, and sodium hypophosphite in a mass ratio of 4:1:1, and the third component is glucose oxidase.

[0047] Comparative Example 1

[0048] This comparative example provides an efficacy composition whose components differ from those of Example 1 only in that the first component is omitted, and it consists of a second component and a third component in a mass ratio of 1.5:0.5. The second component is trisodium phosphate, and the third component is glucose oxidase.

[0049] Comparative Example 2

[0050] This comparative example provides an efficacy composition whose components differ from those of Example 1 only in that the second component is omitted. It consists of a first component and a third component in a mass ratio of 3:0.5. The first component is potassium persulfate, and the third component is glucose oxidase.

[0051] Comparative Example 3

[0052] This comparative example provides an efficacy composition whose components differ from those of Example 1 only in the absence of a third component. It consists of a first component and a second component in a mass ratio of 3:1.5. The first component is potassium persulfate, and the second component is trisodium phosphate.

[0053] Application Example 1

[0054] Field trials were conducted using the efficacy compositions prepared in Examples 1, 5-6, and Comparative Examples 1-3. The specific methods were as follows:

[0055] An experimental plot was selected in the high-quality tobacco area of ​​Qixing Town, Xundian County (Location 1) for the experiment. The specific soil physicochemical properties of the experimental plot are mountain red soil, loam, medium fertility, pH 5.7, available nitrogen 109 mg / kg, available phosphorus 25 mg / kg, available potassium 180 mg / kg, and organic matter content 2.4%.

[0056] The tested variety was Yunyan 87, using floating seedling cultivation. Seedlings were transplanted after 40 days. This experiment employed a randomized block design, dividing the plot into 9 treatment groups. Each treatment group had 200 flue-cured tobacco plants, with a row spacing of 120 cm and a plant spacing of 60 cm, with protective rows. All treatment groups received normal fertilization (fertilizers included tobacco-specific compound fertilizer (12-10-24), 60 kg per mu, applied as 70% basal fertilizer and 30% top dressing; and 10 kg of agricultural potassium nitrate per mu, diluted with water and applied 25 days after transplanting). In addition, different treatment groups were either treated with the efficacy-enhancing compound or not (control group), as detailed below:

[0057] (S1) Apply the efficacy composition of Example 1. On the 8th day after transplanting, apply the second component and the third component, 1.5 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 500 times with water and applied by drip irrigation; then on the 30th day after transplanting, apply the first component and the third component, 3 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 400 times with water and applied by drip irrigation.

[0058] (S2) Apply the efficacy composition of Example 1. On the 15th day after transplanting, apply the second and third components, 1.5 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 500 times with water and applied by drip irrigation; then on the 40th day after transplanting, apply the first and third components, 3 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 400 times with water and applied by drip irrigation.

[0059] (S3) Apply the efficacy composition of Example 1. On the 20th day after transplanting, apply the second component and the third component, 1.5 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 500 times with water and applied by drip irrigation; then on the 45th day after transplanting, apply the first component and the third component, 3 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 400 times with water and applied by drip irrigation.

[0060] (S4) Apply the efficacy composition of Example 5. On the 8th day after transplanting, apply the second component and the third component, 1.5 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 500 times with water and applied by drip irrigation; then on the 30th day after transplanting, apply the first component and the third component, 3 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 400 times with water and applied by drip irrigation.

[0061] (S5) Apply the efficacy composition of Example 6. On the 8th day after transplanting, apply the second component and the third component, 1.5 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 500 times with water and applied by drip irrigation; then on the 30th day after transplanting, apply the first component and the third component, 3 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 400 times with water and applied by drip irrigation.

[0062] (S6) Apply the efficacy composition of Comparative Example 1. On the 8th day after transplanting, apply the second and third components, 1.5 kg / mu for the former and 0.25 kg / mu for the latter, both diluted 500 times with water and then applied by drip irrigation; then on the 30th day after transplanting, apply only the third component, 0.25 kg / mu, diluted 400 times with water and then applied by drip irrigation.

[0063] (S7) Apply the efficacy composition of Comparative Example 2. On the 8th day after transplanting, apply only the third component at 0.25 kg / mu, diluted 500 times with water and then drip-irrigated. Then on the 30th day after transplanting, apply the first and third components at 3 kg / mu and 0.25 kg / mu respectively, diluted 400 times with water and then drip-irrigated.

[0064] (S8) Apply the efficacy composition of Comparative Example 3. On the 8th day after transplanting, apply the second component at 1.5 kg / mu, diluted with water 500 times and then drip irrigated. Then on the 30th day after transplanting, apply the first component at 3 kg / mu, diluted with water 400 times and then drip irrigated.

[0065] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 100 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was cured using the same process. The cured, initially cured tobacco leaves (moisture content 13-17%) were then tested for their main chemical components (1.5 kg sample per group).

[0066] (1) Total sugar content: The detection method is continuous flow method (YC / T 159-2019).

[0067] (2) Reducing sugar content: The detection method is continuous flow method (YC / T 159-2019).

[0068] (3) Total nitrogen content: The detection method is continuous flow method (YC / T 161-2002).

[0069] (4) Nicotine content: The detection method is continuous flow method (potassium thiocyanate) (YC / T 468-2013);

[0070] (5) Potassium content: The detection method is flame photometry (YC / T 173-2003);

[0071] (6) Chlorine content: The detection method is continuous flow method (YC / T 162-2011).

[0072] The results are shown in Table 1.

[0073] Table 1

[0074]

[0075] As shown in Table 1, compared with the control group without the efficacy composition and S6, S7, and S8, the efficacy composition (S1-S5) involved in this invention can significantly reduce the total sugar content, sugar-nitrogen ratio, and sugar-alkali ratio in tobacco leaves, and increase the potassium content in tobacco leaves. The absence of any one of the raw materials will result in a worse effect.

[0076] Comparing the data results of S1 with those of S4-S5, it can be seen that calcium peroxide can help enhance the effect of potassium persulfate in reducing the total sugar content and increasing the potassium content in tobacco leaves, while sodium hexametaphosphate and sodium hypophosphite can help enhance the effect of trisodium phosphate in reducing the total sugar content and increasing the potassium content in tobacco leaves.

[0077] Comparing the data results of S1 and S3, it can be seen that the method of application of the functional composition involved in this invention also affects its effect on reducing the total sugar content and increasing the potassium content in tobacco leaves to a certain extent.

[0078] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 100 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was processed using the same technique. The cured tobacco leaves (moisture content 13-17%) were then shredded. Cigarettes were uniformly hand-rolled using Yunyan (Soft Zhen) empty tubes and stored in a sealed container at (22±1)℃ and 60%±2% relative humidity for at least 48 hours. A 7-person sensory evaluation team assessed the aroma, smoke, and taste characteristics of the sample cigarettes according to the standards in Table 2. The results are shown in Table 3.

[0079] Table 2

[0080]

[0081] Table 3

[0082]

[0083] As shown in Table 3, compared with the control group without the efficacy composition and S6, S7, and S8, the use of the efficacy composition (S1-S5) involved in this invention can significantly improve the sensory quality of tobacco leaves. The absence of any one of the raw materials will result in a worse effect.

[0084] Comparing the data results of S1 with those of S4-S5, it can be seen that calcium peroxide can help enhance the effect of potassium persulfate in improving the sensory quality of tobacco leaves, while sodium hexametaphosphate and sodium hypophosphite can help enhance the effect of trisodium phosphate in improving the sensory quality of tobacco leaves.

[0085] Comparing the data results of S1 and S3, it can be seen that the method of application of the functional composition involved in this invention also affects its effect on improving the sensory quality of tobacco leaves to a certain extent.

[0086] Application Example 2

[0087] Field trials were conducted using the efficacy composition prepared in Example 2, and the specific method was as follows:

[0088] An experimental plot was selected at the Tobacco Technology Extension Station of Rende Subdistrict Office, Xundian County (Location 2) for testing. The specific soil physicochemical properties of the experimental plot were paddy soil, loam, medium fertility, pH 7.7, available nitrogen 90 mg / kg, available phosphorus 40 mg / kg, available potassium 160 mg / kg, and organic matter content 2.6%.

[0089] The tested variety was HP01 (a derivative of Honghua Dajinyuan). Floating seedling cultivation was used, and seedlings were transplanted after 40 days. This experiment employed a randomized block design, dividing the plot into one treatment group and one control group. Each group was planted with 200 flue-cured tobacco plants, with a row spacing of 120 cm and a plant spacing of 55 cm, and protective rows were provided. All groups received normal fertilization (fertilizers included tobacco-specific compound fertilizer (12-10-24), at a rate of 55 kg per mu, applied as 50% as basal fertilizer and 50% as top dressing; and agricultural potassium nitrate at a rate of 10 kg per mu, diluted with water and applied 25 days after transplanting). The treatment group received an additional efficacy-enhancing compound fertilizer, as detailed below:

[0090] The efficacy composition of Example 2 was applied on the 10th day after transplanting. The second and third components were applied at a rate of 1.0 kg / mu for the second component and 0.4 kg / mu for the third component. Both components were diluted 500 times with water and then applied by drip irrigation. On the 35th day after transplanting, the first and third components were applied at a rate of 5 kg / mu for the first component and 0.4 kg / mu for the third component. Both components were diluted 400 times with water and then applied by drip irrigation.

[0091] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 100 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was cured using the same process. The cured, initially cured tobacco leaves (moisture content 13-17%) were then tested for major chemical components (1.5 kg sample per group). The testing method was the same as in Application Example 1.

[0092] The results are shown in Table 4.

[0093] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 100 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was processed using the same technique. The cured tobacco leaves (moisture content 13-17%) were then shredded and uniformly hand-rolled into cigarettes using Yunyan (Soft Zhen) empty tubes. These cigarettes were then sealed and stored for at least 48 hours at a temperature of (22±1)℃ and a relative humidity of 60%±2%. A 7-person sensory evaluation team assessed the aroma, smoke, and taste characteristics of the sample cigarettes according to the standards in Table 2. The results are shown in Table 5.

[0094] Application Example 3

[0095] Field trials were conducted using the efficacy composition prepared in Example 3, and the specific method was as follows:

[0096] An experimental plot was selected in the high-quality tobacco area (location 3) of Banqiao Subdistrict, Shilin County. The specific soil physicochemical properties of the experimental plot were paddy soil, loam, medium fertility, pH 7.2, available nitrogen 118.9 mg / kg, available phosphorus 45 mg / kg, available potassium 188 mg / kg, and organic matter content 2.9%.

[0097] The tested variety was Honghua Dajinyuan, using floating seedling cultivation. Seedlings were transplanted after 40 days of cultivation. This experiment employed a randomized block design, dividing the plot into one treatment group and one control group. Each group was planted with 200 flue-cured tobacco plants, with a row spacing of 120 cm and a plant spacing of 60 cm, including protective rows. All groups received normal fertilization (fertilizers included 50 kg / mu of tobacco-specific compound fertilizer (8-10-24) and 10 kg / mu of agricultural potassium nitrate, applied as a 50 / 50 mixture of compound fertilizer as base fertilizer and top dressing; and potassium nitrate applied once diluted with water 25 days after planting). The treatment group received an additional efficacy-enhancing compound fertilizer, as detailed below:

[0098] The efficacy composition of Example 3 was applied. On the 7th day after transplanting, the second and third components were applied at 3.0 kg / mu for the former and 0.6 kg / mu for the latter, both diluted 500 times with water and then applied by drip irrigation. Then, on the 35th day after transplanting, the first and third components were applied at 2 kg / mu for the former and 0.6 kg / mu for the latter, both diluted 400 times with water and then applied by drip irrigation.

[0099] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 95 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was cured using the same process. The cured, initially cured tobacco leaves (moisture content 13-17%) were then tested for major chemical components (1.5 kg sample per group). The testing method was the same as in Application Example 1.

[0100] The results are shown in Table 4.

[0101] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 95 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was processed using the same technique. The cured tobacco leaves (moisture content 13-17%) were then shredded and uniformly hand-rolled into cigarettes using Yunyan (Soft Zhen) empty tubes. These cigarettes were then sealed and stored for at least 48 hours at a temperature of (22±1)℃ and a relative humidity of 60%±2%. A 7-person sensory evaluation team assessed the aroma, smoke, and taste characteristics of the sample cigarettes according to the standards in Table 2. The results are shown in Table 5.

[0102] Application Example 4

[0103] The efficacy composition prepared in Example 4 was used in a field trial, and the specific method was as follows:

[0104] An experimental plot was selected in the high-quality tobacco area of ​​Guzhen Town, Yiliang County (Location 4) for the experiment. The specific soil physicochemical properties of the experimental plot are mountain purple soil, loam, medium fertility, pH 5.6, available nitrogen 98.5 mg / kg, available phosphorus 34.5 mg / kg, available potassium 256 mg / kg, and organic matter content 2.2%.

[0105] The tested variety was HP01 (a derivative of Honghua Dajinyuan). Floating seedling cultivation was used, and seedlings were transplanted after 40 days. This experiment employed a randomized block design, dividing the plot into one treatment group and one control group. Each group was planted with 200 flue-cured tobacco plants, with a row spacing of 120 cm and a plant spacing of 65 cm, and protective rows were provided. All groups received normal fertilization (fertilizers included tobacco-specific compound fertilizer (12-10-24), at a rate of 50 kg per mu, applied as 70% as basal fertilizer and 30% as topdressing; and agricultural potassium nitrate at a rate of 10 kg per mu, diluted with water and applied 25 days after planting). The treatment group received an additional efficacy-enhancing compound fertilizer, as detailed below:

[0106] The efficacy composition of Example 4 was applied. On the 13th day after transplanting, the second and third components were applied at a rate of 1.0 kg / mu for the second component and 0.25 kg / mu for the third component. Both components were diluted 500 times with water and then applied by drip irrigation. On the 40th day after transplanting, the first and third components were applied at a rate of 4 kg / mu for the first component and 0.25 kg / mu for the third component. Both components were diluted 400 times with water and then applied by drip irrigation.

[0107] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 95 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was cured using the same process. The cured, initially cured tobacco leaves (moisture content 13-17%) were then tested for major chemical components (1.5 kg sample per group). The testing method was the same as in Application Example 1.

[0108] The results are shown in Table 4.

[0109] Mature fresh tobacco leaves from the middle section (leaf positions 9-13 from bottom to top) of each group were harvested 95 days after transplanting. Following the local high-quality tobacco "three-stage" curing process, each group was processed using the same technique. The cured tobacco leaves (moisture content 13-17%) were then shredded and uniformly hand-rolled into cigarettes using Yunyan (Soft Zhen) empty tubes. These cigarettes were then sealed and stored for at least 48 hours at a temperature of (22±1)℃ and a relative humidity of 60%±2%. A 7-person sensory evaluation team assessed the aroma, smoke, and taste characteristics of the sample cigarettes according to the standards in Table 2. The results are shown in Table 5.

[0110] Table 4

[0111]

[0112] As shown in Table 4, compared with the control group without the efficacy composition, the use of the efficacy compositions of Examples 2-4 of this invention (treatment group) can significantly reduce the total sugar content, sugar-nitrogen ratio, sugar-alkali ratio, and increase the potassium content in tobacco leaves.

[0113] Table 5

[0114]

[0115] As shown in Table 5, compared with the control group without the efficacy composition, the use of the efficacy compositions of Examples 2-4 of this invention (treatment group) can significantly improve the sensory quality of tobacco leaves.

[0116] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0117] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0118] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A composition that reduces the total sugar content and increases the potassium content of tobacco leaves, characterized in that, The efficacy composition comprises a first component, a second component, and a third component; The first component includes potassium persulfate; the second component includes trisodium phosphate; and the third component includes glucose oxidase.

2. The efficacy composition according to claim 1, characterized in that, The mass ratio of the first component, the second component, and the third component is (2-5):(1-3):(0.25-1).

3. The efficacy composition according to claim 1 or 2, characterized in that, The first component also includes any one or a combination of at least two of potassium bisulfate, potassium sulfate, potassium peroxymonosulfate, or calcium peroxide; Preferably, the first component comprises a combination of potassium persulfate and calcium peroxide; Preferably, the mass ratio of potassium persulfate to calcium peroxide is (1-5):

1.

4. The efficacy composition according to claim 1 or 2, characterized in that, The second component also includes any one or a combination of at least two of disodium hydrogen phosphate, sodium hexametaphosphate, or sodium hypophosphite; Preferably, the second component comprises a combination of trisodium phosphate, sodium hexametaphosphate, and sodium hypophosphite; Preferably, the mass ratio of trisodium phosphate, sodium hexametaphosphate and sodium hypophosphite is (3-5):(1-2):

1.

5. The use of the efficacy composition according to any one of claims 1-4 in tobacco cultivation.

6. The use of the efficacy composition according to any one of claims 1-4 in reducing the total sugar content and increasing the potassium content of tobacco leaves.

7. The use of the efficacy composition according to any one of claims 1-4 in reducing the sugar-nitrogen ratio and the sugar-alkali ratio of tobacco leaves.

8. A cultivation method for reducing the total sugar content and increasing the potassium content of tobacco leaves, characterized in that, The cultivation method includes: after transplanting tobacco seedlings, applying the efficacy composition of any one of claims 1-4 on the basis of normal fertilization.

9. The cultivation method according to claim 8, characterized in that, The application method of the efficacy composition includes: applying the second and third components of the efficacy composition at any time from the day of transplanting to 15 days after transplanting, wherein the total amount of the second component is 1-3 kg / mu and the total amount of the third component is 0.25-1 kg / mu. Apply the first and third components of the efficacy composition at any time within 30 to 40 days after transplanting, wherein the total amount of the first component is 2-5 kg / mu and the total amount of the third component is 0.25-1 kg / mu.

10. The cultivation method according to claim 8 or 9, characterized in that, The first, second, and third components of the efficacy composition are all applied after being diluted with water, and the dilution ratio is independently selected from any value between 300 and 500 times.

Images