Plant source extract for inhibiting multi-drug-resistant bacteria and application of plant source extract in intestinal flora balance
By combining and differentially extracting three plant extracts, various dosage forms were prepared, which solved the problems of inhibiting multidrug-resistant bacteria and balancing intestinal flora, and achieved safe and effective regulation of intestinal microecology.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- DALIAN MEDICAL UNIVERSITY
- Filing Date
- 2025-12-23
- Publication Date
- 2026-04-17
AI Technical Summary
Existing technologies are insufficient to effectively inhibit multidrug-resistant bacteria and restore the balance of gut microbiota. Traditional antibiotics exacerbate drug resistance, while probiotic preparations have limited effects and are easily affected by the host environment.
By employing a specific combination of three plant extracts, and through multi-target and multi-pathway synergistic effects, combined with differentiated extraction and optimized ratios, the active ingredients are prepared into dosage forms such as enteric-coated capsules, microencapsulated granules, or powders to ensure targeted release of active ingredients in the intestines.
It effectively inhibits multidrug-resistant bacteria, promotes the growth of beneficial bacteria, restores the balance of intestinal flora, reduces the risk of drug resistance, and improves bioavailability and ease of use.
Smart Images
Figure CN121868359A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the fields of biomedicine and microecological regulation technology, specifically to plant-derived extracts that inhibit multidrug-resistant bacteria and their application in the balance of intestinal flora. Background Technology
[0002] With the widespread use and even abuse of antibiotics in medical, livestock, and other fields, the emergence and spread of multidrug-resistant organisms (MDROs) has become a global public health challenge. MDROs, especially Enterobacteriaceae resistant to multiple antibiotics (such as Escherichia coli and Klebsiella pneumoniae), can cause difficult-to-treat infections, significantly increasing the difficulty of treatment, medical costs, and risk of death for patients. The human gut is an important reservoir for the colonization and reproduction of MDROs, and an imbalance in the gut microbiota (i.e., excessive proliferation of harmful bacteria and reduced beneficial bacteria) is closely related to various intestinal and systemic diseases.
[0003] Currently, clinical treatment options for multidrug-resistant bacterial infections are very limited, mainly relying on a few high-level antibiotics (such as carbapenems and polymyxins). However, the use of these drugs further exacerbates the evolution of bacterial resistance and carries risks of side effects such as nephrotoxicity and neurotoxicity. On the other hand, traditional probiotic preparations are used to regulate the gut microbiota, but their targets are relatively singular, and their effectiveness in inhibiting already colonized, highly resistant bacteria is limited. Furthermore, the survival rate and colonization ability of probiotics are easily affected by the host's gut environment, and their stability needs to be improved.
[0004] Against this backdrop, the search for highly effective, low-toxicity, and drug-resistant antibacterial and microecological regulating components from natural plants has become a research hotspot. Plant-derived extracts, due to their rich content of bioactive components (such as polyphenols, flavonoids, alkaloids, and terpenes), often exhibit multi-target mechanisms of action, showing promising application prospects as they may have minimal or even beneficial effects on the host's beneficial flora while inhibiting pathogens. However, the antibacterial spectrum and efficacy of single plant extracts are often limited, making it difficult to effectively combat complex multidrug-resistant bacterial infections. The synergistic effects of different extracts and delivery technologies tailored to the specific intestinal environment (such as avoiding gastric acid degradation and achieving targeted intestinal release) are key factors restricting their practical application.
[0005] Therefore, there is an urgent need in the field to develop a novel, plant-based composition that can effectively and specifically inhibit multidrug-resistant pathogens in the gut while promoting the growth of beneficial bacteria (such as bacteria that produce short-chain fatty acids), thereby safely and effectively restoring a healthy gut microbiota balance. Summary of the Invention
[0006] The purpose of this invention is to provide plant-derived extracts that inhibit multidrug-resistant bacteria and their application in intestinal flora balance. Through a dual mechanism of action that inhibits multidrug-resistant pathogens in the gut and promotes the growth of beneficial bacteria, the invention safely and effectively restores and maintains intestinal flora balance.
[0007] To achieve the above objectives, this invention utilizes the following technical solution: a plant-derived extract for inhibiting multidrug-resistant bacteria and its application in gut microbiota balance. The core lies in determining the compositional basis of the active ingredients, namely, a specific combination of three plant extracts. Its innovation lies not only in the combination itself, but also in the complex biological problem it addresses—the inhibition of multidrug-resistant bacteria and the overall balance of the gut microbiota. This means that the design of this composition is not a simple superposition of antibacterial substances, but rather based on a deep understanding of resistance mechanisms (such as inhibition of drug efflux pumps, biofilm disruption, and interference with drug resistance gene expression) and microecological interactions (such as nutrient competition, colonization resistance, and metabolite regulation). It aims to achieve a comprehensive effect that is difficult to achieve with a single component through the synergistic action of multiple targets and pathways.
[0008] Furthermore, water extraction is suitable for polysaccharides and polyphenols that may exist in the roots and stems of plant A and have immunomodulatory or direct pathogen-inhibiting effects; alcohol extraction can effectively dissolve flavonoids and terpenoids with strong antibacterial activity commonly found in the pericarp of plant B; and supercritical fluid extraction (such as using carbon dioxide) is particularly suitable for volatile components in the leaves of plant C that are easily damaged or oxidized by heat, ensuring that their antibacterial and intestinal motility-regulating activities are not destroyed. This differentiated extraction strategy targeting the chemical characteristics of different plant parts is a key process step to ensure the stable and efficient efficacy of the composition.
[0009] Furthermore, the compounding ratio is not arbitrarily set, but based on systematic pharmacodynamic evaluation and microecological impact assessment. In vitro experiments are used to screen the ratio with the best inhibitory effect on target drug-resistant bacteria (such as carbapenem-resistant Enterobacteriaceae); in vivo microbiota analysis is used to verify whether this ratio has a protective or growth-promoting effect on beneficial bacteria such as Bifidobacteria and Lactobacillus in the intestine. The optimized ratio is the core of achieving the selective effect of "suppressing harm and promoting benefit," avoiding the dysbiosis problem often caused by broad-spectrum antibacterial agents.
[0010] Furthermore, light-protected and low-temperature operation is to prevent the degradation of photosensitive and heat-sensitive active ingredients. Three-dimensional mixing ensures that extract powders of different densities and particle sizes can be fully and uniformly mixed, avoiding component separation. Clearly defined process parameters (temperature, light, time) and subsequent packaging requirements are key points for ensuring that the active ingredients in the composition remain consistent and for effective quality control from production to the end user.
[0011] Furthermore, its possible mechanism of action was revealed. Unlike the direct killing effect of traditional antibiotics, this composition may enhance the body's ability to clear drug-resistant bacteria and reduce their pathogenicity by disrupting biofilms (reducing drug resistance), inhibiting drug-resistant enzymes such as β-lactamase, or blocking communication between bacteria (quorum sensing). This provides a clear theoretical direction for product development.
[0012] Furthermore, it highlights that the composition targets the most challenging multidrug-resistant strains in clinical treatment. The specific antibiotic classes and genera involved enhance the clarity and specificity of the claims, demonstrating the potential value of this composition in solving current clinical problems.
[0013] Furthermore, its mechanism of action may not be directly as a probiotic, but rather by inhibiting competitors (pathogens) or providing specific compounds that are not easily utilized by pathogens but can be fermented by beneficial bacteria (acting as prebiotics), thereby indirectly creating an intestinal environment conducive to the growth of beneficial bacteria.
[0014] Furthermore, short-chain fatty acids (SCFAs) are key indicators of gut health, especially butyrate, which is crucial for intestinal epithelial barrier function, anti-inflammation, and prevention of colorectal cancer. The fact that the composition promotes the production of these specific bacterial genera and SCFAs provides a solid scientific basis for its application in maintaining intestinal barrier integrity, anti-inflammation, and metabolic health.
[0015] Furthermore, the composition is specified as a product (regulator), and its dosage form (oral) and necessary excipients are defined. It is specifically noted that prebiotics (such as fructooligosaccharides) may be added, which synergizes with the composition's own "benefit-promoting" function to jointly optimize the effect of regulating the gut microbiota, reflecting the overall design concept of the product formulation.
[0016] Furthermore, enteric-coated capsules and microencapsulated particles are designed to protect the active ingredients from gastric acid degradation, ensuring efficient delivery to the site of action—the intestines. Microencapsulated particles also enable controlled release, prolonging the duration of action. Special treatments for powders (such as adding silica to improve flowability and using taste masking agents) consider practical ease of use and patient compliance. These specific dosage form designs are crucial for ensuring the final bioavailability and efficacy of the product.
[0017] This invention provides plant-derived extracts that inhibit multidrug-resistant bacteria and their application in intestinal flora balance, which have the following beneficial effects: 1. Highly Effective Inhibition of Multidrug-Resistant Pathogens: This plant-derived composition, through the compounding of extracts from plant A, plant B, and plant C, can specifically inhibit multidrug-resistant bacteria in the individual's gut, such as Enterobacteriaceae resistant to multiple antibiotics. This is due to the synergistic effect of the three plant extracts, which can disrupt the cell membrane integrity of drug-resistant bacteria or interfere with their metabolic pathways, thereby reducing the number of pathogens. This inhibitory effect is not only direct and effective but also avoids the problem of increased drug resistance that may be caused by traditional antibiotics, providing a sustainable solution for maintaining gut health. The natural source of the extracts ensures a gentle process of action, reducing the risk of damage to host cells.
[0018] Promoting the proliferation of beneficial bacteria to optimize the gut microbiota: This composition significantly promotes the growth of beneficial bacteria, particularly those that produce short-chain fatty acids, while inhibiting harmful bacteria. These beneficial bacteria enhance the production of short-chain fatty acids (such as butyrate) through the active ingredients in the fermentation extract, thereby improving intestinal barrier function and regulating immune responses. This bidirectional regulatory effect—inhibiting pathogens and supporting beneficial bacteria—helps to rapidly restore gut microbiota balance and enhance microbial diversity. Compared to single-acting formulations, this composition achieves more comprehensive gut microbiota regulation through the combination of natural ingredients, supporting long-term gut stability.
[0019] The natural plant-derived ingredients offer high safety and low side effects: Because the active ingredients are entirely composed of extracts from plant A, plant B, and plant C, and the extraction processes, such as water extraction, alcohol extraction, and supercritical fluid extraction, avoid residual chemically synthesized reagents, this composition exhibits excellent biocompatibility and safety. This natural characteristic reduces the risk of gastrointestinal irritation, allergic reactions, or drug resistance during use, making it particularly suitable for long-term use in susceptible populations. The plant-derived combination also provides abundant natural active substances such as polyphenols and flavonoids, which, while antibacterial, also possess antioxidant and anti-inflammatory effects, further enhancing overall health benefits.
[0020] The preparation process is stable and maintains the integrity of the active ingredients: Through the specific preparation steps described in the claims, such as physical mixing under light-protected and low-temperature conditions, and the use of customized extraction methods for different plant parts (e.g., water extraction of plant roots and stems, alcohol extraction of fruit peels, and supercritical fluid extraction of leaves), the composition ensures the efficient retention and homogeneity of the active ingredients in each extract. This process design avoids degradation caused by high temperatures or light exposure, resulting in a final mixture with consistent antibacterial and probiotic effects. The physical mixing method also prevents interference from chemical reactions, simplifies the production process, and facilitates large-scale preparation and quality control.
[0021] The flexible dosage form design facilitates practical application: This composition can be formulated into various oral dosage forms, such as enteric-coated capsules, microencapsulated granules, or powders. These dosage forms effectively protect the active ingredients from gastric acid degradation, ensuring targeted release to the intestinal site. This design enhances bioavailability, allowing the composition to precisely target the gut microbiota, thereby strengthening its effects in inhibiting drug-resistant bacteria and regulating the gut microbiota. Furthermore, the diversity of dosage forms allows for customization based on individual needs (e.g., children, the elderly), improving ease of use and adherence. The oral form also facilitates integration into daily diets or supplements, supporting personalized health management. Attached Figure Description
[0022] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.
[0023] Figure 1 This is a flowchart illustrating the composition of the active ingredients in the composition of the present invention. Figure 2 This is a flowchart of the extract preparation method of the present invention; Figure 3 This is a flowchart illustrating the preparation process of the compounded composition of the present invention. Figure 4 This is a flowchart illustrating the preparation and action of the product used in this invention to inhibit multidrug-resistant bacteria; Figure 5 This is a flowchart illustrating the preparation and action of the product used in this invention to promote the growth of beneficial bacteria. Detailed Implementation
[0024] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numerals in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this disclosure. Rather, they are merely examples of apparatuses consistent with some aspects of this disclosure as detailed in the appended claims.
[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.
[0026] How to use: The plant-derived composition is administered orally and is suitable for individuals requiring regulation of gut microbiota balance. The method of use varies depending on the product formulation. If the dosage form is enteric-coated capsules, it is recommended that individuals take 1-2 capsules 1-2 times daily, with water after meals, to ensure that the composition is released in the intestines.
[0027] If the dosage form is microencapsulated granules, the granules can be taken orally directly or mixed with warm water or liquid food, 1-2 times a day, with each intake being 0.5-1.0 grams.
[0028] If the dosage form is powder, it can be dissolved in water or beverage, stirred well and taken orally, 1-2 times a day, each dose being 0.5-1.0 grams.
[0029] Dosage should be adjusted according to individual circumstances (such as age, weight, and gut health) to inhibit multidrug-resistant pathogens (such as Enterobacteriaceae) and promote the growth of beneficial bacteria (such as bacteria that produce short-chain fatty acids). It is recommended to use continuously for 2-4 weeks, or follow professional guidance to determine the course of treatment.
[0030] During use, it should be stored in a cool, dry, and dark place to maintain the stability of the composition.
[0031] Example: Example 1 This embodiment details the preparation and application method of a plant-derived composition for regulating the gut microbiota of elderly individuals. This group is prone to gut microbiota imbalance due to decreased physiological function and potential chronic diseases, and the number of drug-resistant pathogens in the gut may increase.
[0032] First, the raw materials are prepared and processed. High-quality, mold-free dried rhizomes of plant A are selected. After washing, they are placed in a cool, ventilated place to further reduce moisture. Then, they are crushed into coarse particles using specialized equipment to increase the contact area during extraction. For plant B, its dried pericarp is taken, internal impurities are removed, and it is similarly crushed. For plant C, its dried leaves are selected, ensuring the leaves have a natural color and are not yellowed. After sorting, they are lightly rubbed to slightly break down their structure, facilitating the release of active ingredients.
[0033] Next comes the core extraction process. Plant A root and rhizome granules are extracted using water extraction: an appropriate amount of raw material is placed in an extraction tank, sufficient purified water is added, and after soaking for a period of time, it is heated to a gentle boil and maintained for a certain time, with occasional stirring. Then, it is filtered through multiple layers of filter cloth, and the filtrate is collected. The residue is extracted again with water, and the two filtrates are combined. The extract is obtained by low-temperature vacuum concentration and further dried to obtain the extract of plant A. Plant B pericarp is extracted using alcohol: a certain concentration of edible ethanol is used as a solvent, mixed with the raw material, and then heated under reflux for extraction. The temperature is controlled to avoid excessive ethanol volatilization. After filtration, the ethanol is recovered to obtain the alcohol extract of plant B, which is also dried. Plant C leaves are extracted using supercritical fluid extraction: the treated leaves are placed in an extraction vessel, and carbon dioxide is used as the fluid. Extraction is carried out under specific pressure and temperature conditions. Subsequently, the conditions are changed in a separation vessel to vaporize the carbon dioxide, precipitating the plant C extract rich in the target components.
[0034] After the extracts are prepared, they are accurately weighed according to the specific weight ratios described in the claims. The extract powders of plant A, plant B, and plant C are placed together in a mixing device. The mixing process is carried out in a low-temperature environment and under strict light protection, using a three-dimensional rotary mixing method, for a sufficiently long time until all components are fully dispersed, forming a mixture with uniform color and consistent texture.
[0035] This mixture is then uniformly combined with an appropriate amount of food-acceptable carriers such as microcrystalline cellulose and starch, and then encapsulated into enteric-coated capsules using an encapsulation mechanism. These capsules effectively protect the composition from stomach acid, ensuring the release of the active ingredients upon arrival in the intestines.
[0036] Directions for use: Elderly individuals who require this product are advised to take two capsules twice daily, morning and evening, with warm water after meals. Continued use for several weeks can help observe its inhibitory effect on multidrug-resistant pathogens in the gut (such as certain Enterobacteriaceae), while simultaneously supporting the growth and colonization of beneficial gut bacteria that produce short-chain fatty acids, thereby gradually improving the gut microbiota balance and supporting overall health.
[0037] Example 2 This embodiment focuses on the preparation and application of a plant-derived composition microencapsulated granule form suitable for patients in the postoperative recovery period after intestinal surgery. This population has a fragile intestinal environment and is prone to dysbiosis and infection by drug-resistant bacteria.
[0038] The preparation process begins with the rigorous screening and pretreatment of three plant materials. The dried rhizomes of plant A are washed, dried, and then ground into a fine powder using low-temperature pulverization technology for water extraction. The dried pericarp of plant B requires the removal of the inner white membrane, slicing, air-drying, and then pulverizing. The dried leaves of plant C require rapid air-drying immediately after harvesting to retain their activity; they are then gently crushed before use.
[0039] The extraction process followed established methods. Water extraction of plant A root and rhizome powder was performed using a dynamic circulating extraction method, controlling water temperature and extraction time. The extract was centrifuged, filtered through a membrane, and then spray-dried to obtain a fine powder of plant A water extract. Gradient alcohol extraction of plant B pericarp was used: first, the extract was moistened with a lower concentration of ethanol, then subjected to hot reflux extraction with a higher concentration of ethanol. The extract was filtered, concentrated under reduced pressure, and vacuum dried to obtain the plant B alcohol extract. Supercritical carbon dioxide extraction of plant C leaves was used, optimizing extraction pressure and temperature parameters, and collecting the precipitated yellow oleoresin-like extract.
[0040] The mixture is then compounded and microencapsulated. The three extracts are precisely weighed according to a specific ratio, especially the oleoresin-like extract of plant C, which needs to be premixed with a portion of the carrier. The mixed extracts are then mixed with a wall material solution (such as sodium alginate, chitosan, etc.), and microencapsulated particles are prepared by spray drying. This process can embed the active ingredients inside the microcapsules, significantly improving their stability and achieving intestinal-targeted release.
[0041] The resulting microencapsulated particles have good flowability and are easy to dispense. They can be taken orally directly or added to warm water or liquid food.
[0042] Instructions for use: For patients in the postoperative recovery period, it is recommended to take this product once or twice daily under the guidance of a doctor or nutritionist. Take one level spoonful (approximately the prescribed weight) of the microencapsulated granules each time, dissolve in warm water after ingestion, or mix it into a lukewarm liquid diet such as rice water or lotus root starch. This dosage form and usage are designed to act gently on the intestines, helping to control potentially resistant pathogens while supporting the restoration of beneficial bacteria and promoting the reconstruction of intestinal function and flora balance.
[0043] Example 3 This embodiment provides a preparation method for a plant-derived composition powder for daily intestinal health care and its application in health management.
[0044] For raw material processing, high-quality plant roots and stems (A), fruit peels (B), and leaves (C) from authentic producing areas are selected. Plant roots and stems (A) are washed, dried using microwave-assisted drying, and then pulverized. Plant fruit peels (B) are required to have a bright color; after drying, they are pulverized and sieved. Plant leaves (C) are collected and placed in a well-ventilated, dry place to air-dry, avoiding direct sunlight, and then gently crushed for later use.
[0045] The extraction process emphasizes the preservation of all components. Plant A is extracted using low-temperature water extraction: prolonged extraction at a lower temperature minimizes the loss of heat-sensitive components. After fine filtration, the extract is freeze-dried to obtain an extract of Plant A that retains more volatile components. Plant B is extracted using mild alcohol extraction: percolation extraction is performed at room temperature, slowly passing the extract through the raw material layer. The percolate is collected, and the ethanol is recovered under reduced pressure to obtain a paste, which is then dried into powder at low temperature. Plant C is still extracted using supercritical fluid extraction to obtain high-purity active components.
[0046] In the compounding process, the three extract powders are mixed at low speed for a long time in a temperature- and humidity-controlled closed mixer according to the designed weight ratio to ensure extremely uniform distribution and obtain the final composition.
[0047] The composition is thoroughly mixed with prebiotic carriers such as soluble dietary fiber (e.g., fructooligosaccharides) to form a powder. This design not only adjusts the flavor but also synergistically promotes the growth of beneficial bacteria.
[0048] Directions for use: Suitable for adults who are concerned about gut health and have daily health maintenance needs. It is recommended to take one small spoonful of powder directly into the mouth with warm water once daily; or dissolve it in about 100 ml of warm water, milk, or juice, stir well, and drink. It can also be sprinkled on yogurt or salads. Long-term use aims to gently maintain the balance of gut microbiota, help inhibit the excessive proliferation of potentially drug-resistant pathogens, and nourish beneficial bacteria in the gut as part of a healthy lifestyle.
[0049] Example 4 This embodiment details the preparation and specific application of a plant-derived enteric-coated capsule for people with long-term irregular eating habits and poor intestinal function.
[0050] In the raw material pretreatment stage, cleanliness and dryness are particularly emphasized. Plant A's roots and stems are rinsed with running water, then placed in an oven for low-temperature forced-air drying, and pulverized into uniform particles. Plant B uses aged, dried fruit peels with a rich aroma; these are pulverized and sieved through a medium sieve. Plant C's leaves are selected from current-year leaves that are free from pests and have a vibrant green color; they are air-dried, sealed, and stored; they are lightly crushed before use.
[0051] Extraction methods were optimized for the characteristics of different raw materials. For plant A, the rhizome underwent ultrasound-assisted water extraction: after ultrasonic pretreatment, conventional water extraction was performed to improve extraction efficiency. The extract was then concentrated and dried to obtain a powder. For plant B, the pericarp was extracted using a combination of ethanol soaking and reflux to fully extract fat-soluble components. After ethanol recovery, an extract was obtained and dried. For plant C, the leaves underwent supercritical extraction, with the addition of an appropriate amount of entrainer to increase the yield of certain polar components.
[0052] The three extracts were weighed strictly according to a specific weight ratio and mixed for several hours in a mixer with a cooling jacket under low temperature and light-proof conditions to ensure no clumping or layering.
[0053] The well-mixed extract is combined with inert excipients such as dicalcium phosphate and talc, and then filled into enteric-coated capsule shells. The enteric coating ensures the capsules do not dissolve in gastric juices and disintegrate and release their contents upon entering the intestines.
[0054] Directions for use: For individuals whose intestinal function is disrupted due to long-term irregular eating habits, it is recommended to take one capsule twice daily, half an hour after breakfast and dinner, with warm water. It is also recommended to maintain a regular sleep schedule and a balanced diet during use. Through continued use, this composition aims to target and regulate the intestinal environment, reduce the chance of colonization by certain drug-resistant Enterobacteriaceae, and promote the proliferation of beneficial bacteria, gradually improving intestinal problems such as bloating and discomfort.
[0055] Example 5 This embodiment describes the preparation of a plant-derived composition for use in specific care settings (such as long-term care facilities) and its application in group intestinal health management. The dosage form is a conveniently dispensed single-dose powder packet.
[0056] Large-scale processing and quality control of raw materials are key. For plant A, the rhizomes are subject to batch inspection standards, including washing, slicing, uniform drying, and large-scale pulverization. For plant B, the pericarps are sourced from a unified source to ensure consistent quality and are centrally pulverized. For plant C, the leaves are procured from designated suppliers, with strict control over harvesting time and drying conditions, and undergoing batch pre-processing.
[0057] The extraction process was appropriately scaled up. Plant A was extracted using a multi-effect countercurrent water extraction device to improve efficiency and yield; the extract was concentrated via membrane and then spray-dried. Plant B underwent alcohol extraction using a large reflux extraction tank, establishing a standard operating procedure; the extract was then dried using a vacuum belt dryer. Plant C was extracted using industrial-grade supercritical fluid extraction equipment to stabilize operating parameters and ensure batch consistency.
[0058] According to the predetermined large-scale formulation, the three extract powders are put into a large three-dimensional motion mixer and mixed for a long time in a temperature-controlled and dehumidified environment to ensure the uniformity of large batches of products.
[0059] The final mixture is combined with a small amount of flavor enhancer (such as natural plant powder) and filler, and then packaged into quantitative single-dose aluminum foil packets using an automatic powder packaging machine. Each packet contains the same amount, making it easy to dispense and take.
[0060] Instructions for use: In long-term care facilities, after a health assessment, one to two packets of powder can be provided daily to individuals in need. To administer, pour the powder contents into a cup, add an appropriate amount of warm water, stir to dissolve, and drink, or mix into their daily liquid diet. This standardized method of use facilitates administration by caregivers, helps maintain the stability of the user's gut microbiota, and group use helps reduce the risk of transmission of drug-resistant gut pathogens, generally supports beneficial bacteria, and promotes overall gut health.
[0061] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. Plant-derived extracts inhibiting multi-resistant bacteria and their use in the balance of the intestinal flora, characterized in that: The active ingredients of the composition consist of extracts of plant A, plant B, and plant C.
2. The plant-derived extract for inhibiting multi-drug resistant bacteria according to claim 1, and the use thereof in the balance of intestinal flora, characterized by: The extract of plant A is obtained from its dried rhizome by water extraction; the extract of plant B is obtained from its dried pericarp by alcohol extraction; and the extract of plant C is obtained from its dried leaves by supercritical fluid extraction.
3. The plant-derived extract inhibiting multi-drug resistant bacteria according to claim 2, and the use thereof in the balance of intestinal flora, characterized by: The extracts of plant A, plant B, and plant C are compounded in a specific weight ratio.
4. The plant-derived extract inhibiting multi-drug resistant bacteria according to any one of claims 1 to 3, and use thereof in intestinal flora balance, characterized in that: Includes the following steps: Each plant extract was prepared separately and then physically mixed under light-protected and low-temperature conditions until a homogeneous mixture was formed.
5. The use of the plant-derived composition according to any one of claims 1-3 in the preparation of a product for inhibiting the number of multidrug-resistant pathogens in an individual's gut.
6. Use according to claim 5, characterized in that: The multidrug-resistant pathogens include, but are not limited to, Enterobacteriaceae bacteria that are resistant to multiple antibiotics.
7. The use of the plant-derived composition according to any one of claims 1-3 in the preparation of products for promoting the growth of beneficial bacteria in an individual's gut.
8. Use according to claim 7, characterized in that: The beneficial bacteria include, but are not limited to, bacteria that can produce short-chain fatty acids.
9. An oral intestinal microecological regulator comprising a plant-derived composition as described in any one of claims 1-3 as an active ingredient, and one or more pharmaceutically or food-grade carriers.
10. The intestinal micro-ecology modulator according to claim 9, characterized in that: Its dosage forms are enteric-coated capsules, microencapsulated granules, or powders.