Traditional Chinese medicine antiviral anti-influenza spray and preparation method thereof

Abstract

The invention provides a traditional Chinese medicine antiviral anti-influenza spray. The traditional Chinese medicine antiviral anti-influenza spray is prepared from the following raw materials in parts by weight: 5-8 parts of amomum tsao-ko, 6-9 parts of clove, 7-10 parts of purple perilla, 8-11 parts of fructus amomi, 9-12 parts of radix angelicae, 10-13 parts of rhizoma atractylodis, 9-12 parts of elsholtzia, 8-11 parts of agastache rugosus, 7-10 parts of anise and 10-13 parts of fennel. According to the traditional Chinese medicines used in the invention, a traditional prescription is innovated through a monarch, minister, assistant and guide theory, the prescription is reasonable, the medicine flavor is refined, the pharmacodynamic material basis is clear, the treatment basis is sufficient, and the traditional Chinese medicine has good antiviral and anti-influenza effects.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine formulation technology, specifically relating to a traditional Chinese medicine antiviral and anti-influenza spray and its preparation method. Background Technology

[0002] The high infection and mortality rates associated with seasonal influenza outbreaks underscore the importance of influenza prevention. However, the market lacks a traditional Chinese medicine (TCM) daily chemical product specifically designed to protect against influenza viruses. With the rapid development of TCM, developing an antiviral anti-influenza spray and its preparation method is crucial to addressing this issue. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide an antiviral anti-influenza spray and its preparation method in view of the shortcomings of the prior art. The antiviral anti-influenza spray prepared by the method has good antiviral and anti-influenza effects, and is convenient to carry, has a wide range of uses, and has a simple preparation process.

[0004] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is: an antiviral and anti-influenza spray, made from the following raw materials in parts by weight: 5-8 parts of cardamom, 6-9 parts of clove, 7-10 parts of perilla, 8-11 parts of amomum villosum, 9-12 parts of angelica dahurica, 10-13 parts of atractylodes lancea, 9-12 parts of elsholtzia ciliata, 8-11 parts of patchouli, 7-10 parts of star anise, and 10-13 parts of fennel.

[0005] Preferably, it is made from the following raw materials in parts by weight: 5 parts cardamom, 6 parts cloves, 7 parts perilla, 8 parts amomum villosum, 9 parts angelica dahurica, 10 parts atractylodes lancea, 9 parts euphorbia humifusa, 8 parts patchouli, 7 parts star anise, and 10 parts fennel.

[0006] The above-mentioned antiviral anti-influenza spray is also provided, and the method includes the following steps:

[0007] S1. Mix and pulverize the following herbs: cardamom, clove, perilla, amomum, angelica, atractylodes, senna, patchouli, star anise, and fennel to obtain mixed medicinal materials. Extract volatile oil from the mixed medicinal materials to obtain mixed volatile oil.

[0008] S2. Add 70% ethanol by mass to the mixed volatile oil obtained in S1 and mix well to obtain a mixed liquid;

[0009] S3. The mixture obtained in S2 is put into a nasal aerosol bottle to make a traditional Chinese medicine antiviral and anti-influenza spray.

[0010] Preferably, the particle size of the pulverized material in S1 is 2-4 mm.

[0011] Preferably, the method for extracting volatile oil in S1 is as follows: after adding water to the mixed medicinal materials, soak for 6 hours, then use steam distillation combined with a volatile oil extractor to heat and reflux for 3-4 hours to obtain the first volatile oil and distilled water. Dissolve the distilled water in ether, then extract with 1% hydrochloric acid, and then redistill to obtain the second crude volatile oil. Mix the first volatile oil and the second crude volatile oil, then dehydrate and dry with anhydrous sodium sulfate, and filter to obtain the mixed volatile oil.

[0012] Preferably, the solid-liquid ratio of the mixed medicinal materials and water is 1:10.

[0013] Preferably, the volume ratio of the mixed volatile oil and ethanol with a mass fraction of 70% in S2 is 1:2.

[0014] The functions of each medicinal material in this invention are as follows:

[0015] Amomum tsao-ko: Amomum tsao-ko is a traditional bulk medicinal material derived from the Amomum tsao-ko plant of the Zingiberaceae family. The medicinal part is the fruit. It has the functions of drying dampness and warming the middle jiao, stopping malaria and eliminating phlegm. It is used for internal obstruction of cold and dampness, abdominal distension and pain, fullness and vomiting, malaria with chills and fever, and epidemic fever. It has the effects of drying dampness and warming the middle jiao, stopping phlegm. Its volatile oil, phenols, flavonoids, diphenylheptane and bicyclononanes have pharmacological effects such as regulating gastrointestinal function, antibacterial, anti-inflammatory, antioxidant, antitumor, hypoglycemic and lipid-regulating effects.

[0016] Clove: Clove is the dried flower bud of the clove tree, a plant in the Myrtaceae family. It is a traditional Chinese medicine that is both food and medicine. It is warm in nature and pungent in taste, and enters the spleen, stomach, lung, and kidney meridians. Clove contains a variety of chemical components, among which volatile oil is the main component. It has a variety of effects, including antibacterial and anti-inflammatory properties, antipyretic and analgesic effects, anticancer properties, antioxidant properties, antidepressant properties, hypoglycemic properties, anthelmintic properties, antiseptic properties, anti-caries properties, and aphrodisiac properties.

[0017] Angelica dahurica: Angelica dahurica is the dried root of Angelica dahurica or Angelica dahurica var. hainanensis, a plant of the Apiaceae family. Angelica dahurica has a wide range of pharmacological effects, including analgesia, antispasmodic, anti-inflammatory, antipyretic, and anticancer effects. Its main chemical components include volatile oils, coumarins, polysaccharides, amino acids, and trace elements. It is mainly used to treat headaches, colds due to wind and cold, leukorrhea, toothache, boils and carbuncles, rhinitis, and other symptoms.

[0018] Perilla: Also known as cinnamon perilla or red perilla, perilla is an annual herb of the Lamiaceae family. It is both a food and a medicine, with its roots, stems, and leaves all used medicinally. It is rich in various nutrients and active substances, possessing anti-inflammatory, antibacterial, antioxidant, anti-allergic, anti-tumor, and lipid synthesis-regulating effects.

[0019] Patchouli: Patchouli is the dried whole herb of the genus Patchouli in the Lamiaceae family, also known as wild patchouli. It contains a variety of chemical components such as volatile oils, flavonoids, and phenolic acids, and has pharmacological effects such as regulating the digestive system, resisting pathogenic microorganisms, anti-inflammatory and antioxidant properties.

[0020] Elsholtzia ciliata: Elsholtzia ciliata is the dried aerial part of the plant Elsholtzia ciliata or Elsholtzia jiangensis, belonging to the Lamiaceae family. It is pungent and slightly warm in nature, and enters the lung, spleen, and stomach meridians. Its main functions are to induce sweating and relieve exterior syndromes, harmonize the middle jiao and resolve dampness, and promote diuresis and reduce swelling. Elsholtzia ciliata is not only rich in volatile oils but also contains high levels of flavonoids, which have antimicrobial, sedative, immune-enhancing, and antioxidant effects.

[0021] Amomum villosum: A traditional Chinese medicine, Amomum villosum is the dried fruit of Amomum villosum, Amomum tsao-ko, or Amomum hainanense, belonging to the ginger family. It is warm in nature, bitter and pungent in taste, and primarily enters the spleen, stomach, and kidney meridians. It is used to treat abdominal pain due to cold, stop dysentery caused by overexertion, aid digestion, and warm the spleen and stomach. It mainly contains volatile oils, flavonoids, polysaccharides, and other bioactive components, possessing antioxidant, blood pressure-lowering, and antibacterial effects.

[0022] Atractylodes lancea: Atractylodes lancea is a perennial herb of the Asteraceae family. It has a pungent and bitter taste and enters the spleen, stomach, and liver meridians. Atractylodes lancea can strengthen the spleen, eliminate dampness, dispel wind, and reduce fever. It can be used to treat rheumatic diseases, digestive system disorders, night blindness, and influenza. Atractylodes lancea has many active medicinal components, the main ones currently identified are: atractylodesin, β-cineole, atractylodesyl alcohol, atractylodes lactone, as well as sugars and sterols, which have anti-gastric ulcer, immunomodulatory, antibacterial, anti-inflammatory, antitumor, antioxidant, and neuroprotective effects.

[0023] Star anise: Star anise is the dried whole plant of *Illicium verum*, a plant in the Magnoliaceae family. It has a pungent taste and warm properties, and enters the liver, kidney, spleen, and stomach meridians. It has the functions of warming yang and dispelling cold, regulating qi and relieving pain, and is used for cold hernia abdominal pain, stomach cold vomiting, and cold pain in the epigastrium and abdomen. It mainly contains volatile oils, flavonoids, trace elements, and other chemical components, and has antibacterial, analgesic, and antiviral properties.

[0024] Fennel: Fennel, a commonly used traditional Chinese medicine, is the dried, mature fruit of the fennel plant (Fennel arvense), a member of the Apiaceae family. It is an annual herb. It is warm in nature, pungent and sweet in taste, and has the functions of regulating qi and stomach, dispelling cold and relieving pain. Therefore, it is often used to treat conditions such as abdominal pain due to cold, dysmenorrhea, poor appetite and vomiting / diarrhea, lower abdominal pain due to cold, abdominal distension and pain, testicular swelling, and hydrocele. The medicinal material mainly contains sterols and glycosides, essential oils, alkaloids, amino acids, and fatty oils, as well as tannins, flavonoids, saponins, triterpenes, and other compounds. It has the effects of relieving pain, anti-inflammation, antibacterial properties, promoting gastrointestinal motility, protecting the liver, and anti-liver fibrosis.

[0025] Compared with the prior art, the present invention has the following advantages:

[0026] 1. In this invention, Atractylodes lancea and Elsholtzia ciliata are used as principal herbs, and Amomum villosum, Foeniculum vulgare, Angelica dahurica and Pogostemon cablin are used as assistant herbs. In accordance with the theory of principal, assistant, adjuvant and guide herbs, this invention innovates traditional prescriptions, with reasonable prescriptions, refined herbs, clear pharmacodynamic material basis, and sufficient therapeutic basis. It has good antiviral and anti-influenza effects.

[0027] 2. Compared with traditional dosage forms such as granules and capsules on the market, this invention innovates the method of administration, which is absorbed through the nasal mucosa to achieve local to systemic prevention and treatment effects; compared with Western medicine, this product is a pure Chinese medicine formula, with no toxic side effects and definite and stable efficacy.

[0028] The present invention will be further described in detail below with reference to the embodiments. Detailed Implementation

[0029] Example 1

[0030] The traditional Chinese medicine antiviral and anti-influenza spray of this embodiment is made from the following raw materials in parts by weight: 5 parts cardamom, 6 parts cloves, 7 parts perilla, 8 parts amomum villosum, 9 parts angelica dahurica, 10 parts atractylodes lancea, 9 parts elsholtzia ciliata, 8 parts patchouli, 7 parts star anise, and 10 parts fennel.

[0031] The preparation method of the above-mentioned traditional Chinese medicine antiviral and anti-influenza spray includes the following steps:

[0032] S1. A mixture of cardamom, cloves, perilla, amomum, angelica, atractylodes, elsholtzia, patchouli, star anise, and fennel is pulverized to a particle size of 2 mm to obtain a mixed medicinal material. Volatile oil is extracted from the mixed medicinal material to obtain a mixed volatile oil. The extraction method is as follows: water is added to the mixed medicinal material and soaked for 6 hours. Then, steam distillation is used in conjunction with a volatile oil extractor, followed by heating and reflux for 3 hours to obtain the first volatile oil and distilled water. The distilled water is dissolved in ether and extracted with 1% hydrochloric acid. Then, the mixture is redistilled using the steam distillation method to obtain the second crude volatile oil. The first and second crude volatile oils are mixed, dehydrated and dried with anhydrous sodium sulfate, and filtered to obtain the mixed volatile oil. The solid-liquid ratio of the mixed medicinal material to water is 1:10.

[0033] S2. Add 70% ethanol by mass to the mixed volatile oil obtained in S1 and mix evenly to obtain a mixed liquid; the volume ratio of the mixed volatile oil to 70% ethanol by mass is 1:2.

[0034] S3. The mixture obtained in S2 is put into a nasal aerosol bottle to make a traditional Chinese medicine antiviral and anti-influenza spray.

[0035] The effects of the traditional Chinese medicine antiviral and anti-influenza spray described in this embodiment on the lungs of mice were investigated.

[0036] Before the experiment: 60 healthy SPF-grade SD mice, 30 males and 30 females, aged 4-6 weeks and weighing (30-34) g, were divided into 6 treatment groups, with 10 mice in each group (5 females and 5 males). The 6 treatment groups included blank group, model group, positive drug group, low-dose group, medium-dose group and high-dose group.

[0037] Experiment began with the following administration: The control group and model group received equal volumes of physiological saline (0.1 mL / 30 g); the positive control group received ribavirin (2 mg / kg); the high-dose group (3.12 g / kg for mice), the medium-dose group (1.56 g / kg for mice), and the low-dose group (0.78 g / kg for mice); all groups (high-dose, medium-dose, and low-dose) received the traditional Chinese medicine antiviral and anti-influenza spray prepared in this embodiment. Administration was repeated for 7 days, once daily. Modeling was initiated 2 hours after the last administration (the mice were fasted for 12 hours prior to modeling, but water was permitted).

[0038] Post-experiment: Mice were sacrificed and samples were collected 24 hours after modeling. Lung tissue from mice was taken, homogenized, centrifuged, and the supernatant was used to detect viral infection and viral load. The experimental records are shown in the table below.

[0039] Table 16 shows the effect of treatments on viral infection in mouse lung tissue.

[0040]

[0041]

[0042] As shown in Table 1, the proportion of organs changes after infection. After the high-dose group was used, the lung infection index of mice was similar to that of the positive drug group, both close to the blank group and much lower than that of the model group. This indicates that the traditional Chinese medicine antiviral and anti-influenza spray prepared in this embodiment has improved the viral infection of mice and reduced the harm of the virus to lung tissue.

[0043] Table 26 shows the effects of treatment groups on viral load in mouse lung tissue.

[0044]

[0045] As shown in Table 2, the viral load in the lung tissue on the seventh day was generally higher than that on the third day. Comparing the model group and the positive drug group, the viral load in the lung tissue of mice after the high-dose group was similar to that of the positive drug group and much lower than that of the model group. This proves that the high-dose group helps to reduce the viral load in the lung tissue, indicating that the traditional Chinese medicine antiviral and anti-influenza spray prepared in this embodiment has a certain antiviral and anti-influenza ability.

[0046] Example 2

[0047] The traditional Chinese medicine antiviral and anti-influenza spray of this embodiment is made from the following raw materials in parts by weight: 8 parts of cardamom, 9 parts of clove, 10 parts of perilla, 11 parts of amomum villosum, 12 parts of angelica dahurica, 13 parts of atractylodes lancea, 12 parts of elsholtzia ciliata, 11 parts of patchouli, 10 parts of star anise, and 13 parts of fennel.

[0048] The preparation method of the above-mentioned traditional Chinese medicine antiviral and anti-influenza spray includes the following steps:

[0049] S1. A mixture of cardamom, cloves, perilla, amomum, angelica, atractylodes, elsholtzia, patchouli, star anise, and fennel is pulverized to a particle size of 4 mm to obtain a mixed medicinal material. Volatile oil is extracted from the mixed medicinal material to obtain a mixed volatile oil. The extraction method is as follows: water is added to the mixed medicinal material and soaked for 6 hours. Then, steam distillation is used in conjunction with a volatile oil extractor, followed by heating and reflux for 4 hours to obtain a first volatile oil and distilled water. The distilled water is dissolved in ether and extracted with 1% hydrochloric acid. Then, the mixture is re-distilled using the same steam distillation method in conjunction with the volatile oil extractor to obtain a second crude volatile oil. The first and second crude volatile oils are mixed, dehydrated and dried with anhydrous sodium sulfate, and filtered to obtain the mixed volatile oil. The solid-liquid ratio of the mixed medicinal material to water is 1:10.

[0050] S2. Add 70% ethanol by mass to the mixed volatile oil obtained in S1 and mix evenly to obtain a mixed liquid; the volume ratio of the mixed volatile oil to 70% ethanol by mass is 1:2.

[0051] S3. The mixture obtained in S2 is put into a nasal aerosol bottle to make a traditional Chinese medicine antiviral and anti-influenza spray.

[0052] In this embodiment, the particle size of the pulverized material in S1 can also be 3 mm; the heating, condensation, and reflux time can also be 3.5 h.

[0053] Example 3

[0054] The traditional Chinese medicine antiviral and anti-influenza spray of this embodiment is made from the following raw materials in parts by weight: 7 parts of cardamom, 8 parts of clove, 8 parts of perilla, 10 parts of amomum villosum, 10 parts of angelica dahurica, 12 parts of atractylodes lancea, 11 parts of elsholtzia ciliata, 10 parts of patchouli, 9 parts of star anise and 12 parts of fennel.

[0055] The preparation method of the traditional Chinese medicine antiviral and anti-influenza spray in this embodiment is the same as that in Example 2.

[0056] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention in any way. Any simple modifications, alterations, and equivalent changes made to the above embodiments based on the inventive essence shall still fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine anti-viral flu-preventing spray, characterized in that, It is made from the following ingredients in parts by weight: 5-8 parts cardamom, 6-9 parts cloves, 7-10 parts perilla, 8-11 parts amomum villosum, 9-12 parts angelica dahurica, 10-13 parts atractylodes lancea, 9-12 parts elsholtzia ciliata, 8-11 parts patchouli, 7-10 parts star anise, and 10-13 parts fennel.

2. The anti-viral flu-preventing Chinese medicine spray of claim 1, characterized in that, It is made from the following ingredients in parts by weight: 5 parts cardamom, 6 parts cloves, 7 parts perilla, 8 parts amomum villosum, 9 parts angelica dahurica, 10 parts atractylodes lancea, 9 parts euphorbia humifusa, 8 parts patchouli, 7 parts star anise, and 10 parts fennel.

3. A method for preparing a traditional Chinese medicine antiviral and anti-influenza spray as described in claim 1 or 2, characterized in that, The method includes the following steps: S1. Mix and pulverize the following herbs: cardamom, clove, perilla, amomum, angelica, atractylodes, senna, patchouli, star anise, and fennel to obtain mixed medicinal materials. Extract volatile oil from the mixed medicinal materials to obtain mixed volatile oil. S2. Add 70% ethanol by mass to the mixed volatile oil obtained in S1 and mix well to obtain a mixed liquid; S3. The mixture obtained in S2 is put into a nasal aerosol bottle to make a traditional Chinese medicine antiviral and anti-influenza spray.

4. The preparation method of a traditional Chinese medicine antiviral and anti-influenza spray according to claim 3, characterized in that, The particle size of the pulverized material described in S1 is 2-4 mm.

5. The preparation method of a traditional Chinese medicine antiviral and anti-influenza spray according to claim 3, characterized in that, The method for extracting volatile oil described in S1 is as follows: after adding water to the mixed medicinal materials and soaking for 6 hours, the mixture is heated and refluxed for 3-4 hours using a steam distillation method combined with a volatile oil extractor to obtain the first volatile oil and distilled water. The distilled water is dissolved in ether and then extracted with 1% hydrochloric acid, followed by redistillation to obtain the second crude volatile oil. The first volatile oil and the second crude volatile oil are mixed, then dehydrated and dried with anhydrous sodium sulfate, and filtered to obtain the mixed volatile oil.

6. The preparation method of a traditional Chinese medicine antiviral and anti-influenza spray according to claim 5, characterized in that, The solid-liquid ratio of the mixed medicinal materials and water is 1:

10.

7. The preparation method of a traditional Chinese medicine antiviral and anti-influenza spray according to claim 3, characterized in that, The volume ratio of the mixed volatile oil and ethanol with a mass fraction of 70% in S2 is 1:2.

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