Organic fertilizer suitable for plant leaf surfaces as well as preparation method and use method of organic fertilizer

Abstract

The invention provides a plant foliar organic fertilizer and a preparation and use method thereof, and relates to the technical field of crop planting. The organic fertilizer solves the problem that an existing foliar fertilizer is difficult to consider crop yield, quality and ecological safety at the same time. The nutrient base liquid contains pomelo kernel pulp, shrimp shell cold soaking liquid, wood root bark suspension liquid, a vitamin composition and a potassium element additive. The preparation method comprises the following steps: preparing the component solutions, mixing, adding the vitamin and potassium additive, stirring at 5-16 DEG C to obtain the nutrient base solution, and mixing with water. The application method comprises the following steps: adjusting the spraying amount according to seasons, wherein the basic amount is 150-200L / hectare; potassium additives are proportioned according to growth parts, and vitamins are added seasonally; the basic amount in spring is increased by 10% in summer and reduced by 10% in autumn, and the vitamin C is doubled; atomization spraying is carried out in the morning or at dusk, and high temperature, strong light and rain are avoided; spraying is performed once every 7-10 days, and 2-3 times is a cycle. Natural organic chromium is adopted, activity is kept through a low-temperature process, seasonal part differentiated matching is achieved, and environment friendliness and high efficiency are achieved.

Description

Technical Field

[0001] This invention relates to the field of crop cultivation technology, specifically to a foliar organic fertilizer for plants and its preparation and application methods. Background Technology

[0002] The application of organic chromium as a beneficial element has been extensively studied: as a core component of glucose tolerance factor (GTF), it can enhance insulin sensitivity, regulate lipid metabolism, and promote growth and development. It has not only been proven to be a feed additive for poultry to improve production performance and meat quality, but also a dietary supplement for type 2 diabetes in humans. Current technologies for obtaining organic chromium include synthesizing high-chromium yeast through culture media; however, these methods often involve artificial intervention and targeted synthesis, resulting in difficulties in controlling component purity and insufficient compatibility with natural organisms.

[0003] In the fertilizer field, traditional foliar fertilizers have many limitations: on the one hand, some products rely on chemically synthesized raw materials or high-temperature purification processes, which can easily damage the original properties of the raw materials and may bring potential side effects; on the other hand, existing fertilizers often directly add exogenous nutrients (such as potassium and chromium), but different organisms have individual differences in their needs for elements, and in most cases, it is impossible to accurately detect the actual threshold of the organism's needs, leading to excessive or insufficient exogenous supplementation, which not only affects plant growth but may also be passed on to animals and humans through the food chain, causing health risks. At the same time, after the Western Industrial Revolution, resource utilization methods have become more extensive, and the wisdom of "recycling and adapting to nature" in traditional agricultural culture has not been fully inherited. Existing fertilizer technologies lag behind the needs of green development in terms of ecological harmony and resource utilization, and there is an urgent need for innovative breakthroughs that combine traditional processes with modern technologies.

[0004] Plants have the ability to synthesize a constant amount of organic chromium. The chromium content varies significantly among different plants. However, how to efficiently extract this natural organic chromium and combine it with other beneficial components to prepare foliar fertilizers that are suitable for plant growth and can ensure the safety of the subsequent food chain through a gentle process has become a key need for current technological research and development. Summary of the Invention

[0005] The purpose of this invention is to provide a plant foliar organic fertilizer with plant-derived natural organic chromium as its core, a mild process, and a precise formulation.

[0006] Another objective of this invention is to provide a specific method for preparing the above-mentioned organic fertilizer suitable for plant foliar application.

[0007] Another objective of this invention is to provide a method for using organic fertilizer suitable for plant foliar application.

[0008] This invention is achieved through the following technical solution: a plant foliar organic fertilizer, which is made by mixing nutrient base solution and water in weight percentages, wherein the nutrient base solution accounts for 32% to 38% and the water accounts for 62% to 68%; the nutrient base solution includes pomelo seed pulp, shrimp shell cold soaking solution, cypress root bark suspension, vitamin composition and potassium element additive.

[0009] The working principle of this technical solution is to utilize "constant organic chromium" synthesized by the plant itself. Chromium is a core component of glucose tolerance factor (GTF), which can enhance insulin sensitivity, regulate lipid metabolism, and promote growth and development. The processing of shrimp shells and cypress root bark incorporates traditional processing techniques to produce a cold-soaked shrimp shell extract and a cypress root bark suspension, which further enriches beneficial components. Vitamins participate in regulating the physiological metabolism of plants, and potassium is an essential macronutrient for plants. This compound formula aims to improve the plant's stress resistance and growth quality through nutritional complementarity.

[0010] To better realize the present invention, the nutrient base solution has different proportions depending on the season, by weight, Spring: 88-90 parts pomelo seed pulp, 1.5-2.0 parts shrimp shell cold soaking solution, 2.0-3.0 parts cypress root bark suspension, 5-7 parts vitamin composition, and 0.03-0.06 parts potassium element additive; Summer: 78-83 parts pomelo seed pulp, 1.5-2.0 parts shrimp shell cold soaking solution, 3.0-4.0 parts cypress root bark suspension, 12-14 parts vitamin composition, and 0.03-0.06 parts potassium element additive; Autumn: 74-78 parts of pomelo seed pulp, 1.5-2.0 parts of shrimp shell cold soaking solution, 2.5-3.5 parts of cypress root bark suspension, 16-18 parts of vitamin composition, and 0.03-0.06 parts of potassium element additive.

[0011] To better realize the present invention, the vitamin composition further comprises vitamin B2, vitamin C and vitamin D3, with each vitamin in a seasonal ratio, by weight. Springtime: Vitamin B2 1.5–2.5 parts, Vitamin C 2.5–3.5 parts, Vitamin D3 0.5–1.5 parts; Summer: Vitamin B2 3.5-4.5 parts, Vitamin C 5.5-6.5 parts, Vitamin D3 2.5-3.5 parts; Autumn: Vitamin B2 5.5-6.5 parts, Vitamin C 8.5-9.5 parts, Vitamin D3 1.5-2.5 parts.

[0012] To better realize the present invention, the potassium element additive is further defined as at least one of potassium humate, potassium citrate, and wood ash leachate. The dosage of the potassium element additive varies with the season and also needs to be adjusted according to the differences in plant parts. By weight, the dosage of potassium element additive is 1.5 parts for root and rhizome plants, 1.8 parts for leaf and flower plants, and 2.3 parts for fruit and seed plants.

[0013] The above-mentioned method for preparing a plant foliar organic fertilizer includes the following steps: Step S1: Prepare pomelo seed pulp, shrimp shell cold soaking solution, and cypress root bark suspension respectively; Step S2: Mix the prepared pomelo seed pulp, shrimp shell cold soaking liquid, and cypress root bark suspension, add vitamin composition and potassium element additive, and stir evenly at 5-16℃ to obtain nutrient base solution. Step S3: Mix the nutrient base solution at a weight percentage of 32%–38% and water at a weight percentage of 62%–68% to obtain the organic fertilizer suitable for plant foliar application.

[0014] The principle of this technical solution is to strictly control the temperature during fermentation and cold soaking at a low temperature of 5-16℃ when preparing pomelo seed pulp, shrimp shell cold soaking solution, and cypress root bark suspension, and to avoid chemical synthesis purification and high-temperature treatment throughout the process, as high temperatures often destroy the protoplasmic structure and natural active substances in plant or animal raw materials. Through low-temperature cold soaking and fermentation, the natural activity and beneficial components of the raw materials (such as chitin in shrimp shells, medicinal components in cypress root bark, and active substances in pomelo seeds) can be preserved to the maximum extent, thereby enhancing the fertilizer's promoting effect on plant growth.

[0015] To better prepare the above-mentioned organic fertilizer suitable for plant foliar application, the specific preparation process of the pomelo seed pulp in step S1 is as follows: Take 200 kg of at least one of pomelo, grapefruit, or grapefruit as raw material, use 20 kg of water and 200 g of baking soda to prepare a cleaning solution, clean the surface of the raw material, and then air dry it naturally. Grind it at a low temperature of 5℃~16℃; add 6 kg of sucrose to the ground raw material, seal it in a container, and ferment it at a low temperature of 5℃~16℃ for at least 384 hours. During the fermentation process, the environment should be kept away from light, and the pressure inside the container should be maintained at normal pressure. Filter to obtain the pomelo seed pulp.

[0016] To better prepare the above-mentioned organic fertilizer suitable for plant foliar application, the specific preparation process of the shrimp shell cold soaking solution in step S1 is as follows: using clean crayfish shells as raw materials, soaking them in purified water for 2 hours, then cutting them into 0.5cm-1cm fragments with a knife or crusher, and placing them in a ventilated and dry environment to air dry naturally, or drying them at a low temperature below 45℃, until the shrimp shell moisture content is ≤5%; then putting the dried shrimp shells into an ultra-fine pulverizer to pulverize them, passing them through an 8-type sieve to obtain extremely fine shrimp shell powder; adding the extremely fine shrimp shell powder to purified water, placing it in a sealed container, and cold soaking it at a low temperature of 5℃~16℃ for at least 384 hours; after cold soaking, allowing it to stand and separate into layers, removing the light impurities floating on the surface and the coarse particles precipitated at the bottom, and only taking the naturally floating liquid in the middle layer, which is the shrimp shell cold soaking solution.

[0017] To better prepare the above-mentioned organic fertilizer suitable for plant foliar application, the specific preparation process of the *Cycas revoluta* root bark suspension in step S1 is as follows: Select *Cycas revoluta* root bark free from mold and pests, and remove surface mud and impurities; under sterile conditions, cut into pieces, dry at low temperature, and pulverize into fine powder, pass through an 8-type sieve, place in a sealed container, add purified water, and cold soak at a low temperature of 5℃~16℃ for at least 384 hours. During the cold soaking process, the container must be kept sealed, and the mixture should be gently stirred once every 24 hours; after the cold soaking is completed, let it stand to allow the liquid to naturally separate into layers, remove the light impurities floating on the surface and the coarse particles precipitated at the bottom, and take the suspension slurry in the middle layer to obtain the *Cycas revoluta* root bark suspension.

[0018] To better prepare the above-mentioned organic fertilizer suitable for plant foliar application, the mixing processes in steps S2 and S3 are further carried out in a stainless steel container lined with a plastic bag and kept sealed throughout the process.

[0019] The above-mentioned method for applying organic fertilizer to plant leaves includes the following steps: (1) According to the season, the organic fertilizer is prepared. The basic spraying amount of organic fertilizer is 150~200L / hectare. In response to the growth needs of plant roots, stems, leaves, flowers, fruits and seeds, the potassium element additive is adjusted according to the weight ratio of 1.5 parts for roots and stems, 1.8 parts for leaves and flowers, and 2.3 parts for fruits and seeds. At the same time, vitamin B2, vitamin C and vitamin D3 are added according to the seasonal ratio. (2) In spring, use the basic amount of organic fertilizer for spraying; in summer, increase the amount by 10% on the basis of the basic amount of fertilizer; in autumn, reduce the amount by 10% on the basis of the basic amount of fertilizer; when spraying in autumn, double the proportion of vitamin C in the vitamin composition. (3) Use a misting sprayer and spray at 6:00-8:00 or 18:00-20:00 every morning, avoiding high temperature and strong light periods and rainy days; if it rains within 4 hours after spraying, re-spraying is required, and the amount of organic fertilizer used should be halved when re-spraying; (4) Spray once every 7 to 10 days, and spray 2 to 3 times in a row for one growth cycle.

[0020] The working principle of this technical solution is that the usage method emphasizes "adapting to the times" and "adapting to local conditions".

[0021] Seasonal Regulation: Based on the different growth and metabolic characteristics of plants in spring, summer, and autumn, the proportions of pomelo seed slurry, shrimp shell cold soaking solution, cypress root bark suspension, and vitamins are adjusted. For example, the proportion of the vitamin combination is increased in summer and autumn. Partial Regulation: Based on the different potassium requirements of different growth parts of plants (roots, leaves, flowers, fruits, and seeds), the proportion of potassium additives is precisely adjusted. Plants have different nutritional needs at different growth stages and during different organ development periods. Dynamically adjusting the proportions aims to precisely match the real-time physiological needs of plants, avoiding nutrient waste or insufficient supply, and achieving optimal fertilization results.

[0022] Compared with the prior art, the present invention has the following advantages and beneficial effects: (1) The core chromium source of this invention is derived from the stable organic chromium synthesized by grapefruit, pomelo, and pomelo. It does not require artificial chemical synthesis, thus avoiding the purity fluctuation and biocompatibility risks of artificially synthesized organic chromium from the source. This natural organic chromium is more in line with the physiological metabolism of plants and is easily absorbed and utilized by plants. This indirect nutrient supplementation mode with plants as carriers is more in line with the metabolic laws of living organisms than the method of directly adding exogenous ingredients. It can provide safe nutritional support for human health while ensuring the growth performance of plants and animals. (2) The present invention uses a low-temperature fermentation process to prepare pomelo seed pulp, shrimp shell cold soaking liquid and cypress root bark suspension. The entire process does not involve high temperature or chemical synthesis and purification steps, thus fully preserving the protoplasm and natural active substances of the raw materials. In addition, the processing of shrimp shells and cypress root bark incorporates traditional processes, further enriching the beneficial components in the raw materials. Compared with traditional high-temperature foliar fertilizers, the present invention has higher biological activity and a more significant effect on promoting plant growth. (3) The present invention adopts a dual regulation mode of seasonal differential ratio and plant part targeted ratio. The component ratio of the nutrient base solution can be dynamically adjusted according to the plant growth needs in spring, summer and autumn. Potassium element additives can be precisely adapted to the growth characteristics of different parts such as roots, stems, leaves, flowers, fruits and seeds. This ratio mode realizes the supply of nutrients on demand and maximizes the plant absorption and utilization rate. (4) This invention follows the core idea of ​​traditional farming to adapt to nature and recycle. The raw materials are all taken from natural plants and agricultural by-products. There are no pollutant emissions during the production process. After application, it will not cause soil compaction or water pollution. This makes the traditional process and modern technology organically integrated, effectively improving the resource utilization rate and meeting the needs of green agriculture and ecological sustainable development. Attached Figure Description

[0023] Other features, objects, and advantages of the invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings: Figure 1 This is a photograph of a cabbage experimental field in Example 2 of the present invention that was not sprayed with organic fertilizer; Figure 2 This is a photograph of a cabbage experimental field after organic fertilizer was sprayed in Example 2 of the present invention; Figure 3 This is a photograph of an Ophiopogon japonicus experimental field in Example 3 of the present invention, where no organic fertilizer was sprayed. Figure 4 This is a photograph of the growth of *Ophiopogon japonicus* without organic fertilizer in Example 3 of the present invention; Figure 5 This is a photograph of the Ophiopogon japonicus experimental field after organic fertilizer was sprayed in Example 3 of the present invention; Figure 6 This is a photograph showing the growth of Ophiopogon japonicus after spraying organic fertilizer in Example 3 of the present invention. Figure 7 This is a photograph showing the growth of wheat after spraying organic fertilizer in Example 4 of the present invention. Detailed Implementation

[0024] To make the objectives, process conditions, and advantages of the present invention clearer, the present invention will be further described in detail with reference to the following embodiments. However, the embodiments of the present invention are not limited thereto. Various substitutions and modifications can be made based on common technical knowledge and conventional means in the art without departing from the above-described technical concept of the present invention, and all such substitutions and modifications should be included within the scope of the present invention. The specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0025] This invention provides an organic fertilizer suitable for plant foliar application. The organic fertilizer is made by mixing a nutrient base solution and water in weight percentages, wherein the nutrient base solution accounts for 32% to 38% and the water accounts for 62% to 68%. The nutrient base solution includes pomelo seed pulp, shrimp shell cold soaking solution, cypress root bark suspension, vitamin composition and potassium element additive.

[0026] The method for preparing this organic fertilizer includes the following steps: Step S1: Prepare pomelo seed pulp, shrimp shell cold soaking solution, and cypress root bark suspension respectively; The specific preparation process of pomelo seed pulp is as follows: Take 200 kg of at least one of pomelo, grapefruit or grapefruit as raw material, use 20 kg of water and 200 g of baking soda to prepare a cleaning solution, clean the surface of the raw material, and then air dry it naturally. Grind it in a low temperature environment of 5℃~16℃. Add 6 kg of sucrose to the ground raw material, seal it in a container and ferment it in a low temperature environment of 5℃~16℃ for at least 384 hours. During the fermentation process, the environment should be kept away from light and the pressure inside the container should be maintained at normal pressure. Filter to obtain pomelo seed pulp.

[0027] The specific preparation process of shrimp shell cold soaking solution is as follows: using clean crayfish shells as raw materials, soaking them in purified water for 2 hours, then cutting them into 0.5cm-1cm fragments with a knife or crusher, and placing them in a ventilated and dry environment to air dry naturally, or drying them at a low temperature below 45℃, until the shrimp shell moisture content is ≤5%; then putting the dried shrimp shells into an ultra-fine pulverizer to pulverize them, passing them through an 8-type sieve to obtain extremely fine shrimp shell powder; taking the extremely fine shrimp shell powder and adding it to purified water, placing it in a sealed container, and cold soaking it at a low temperature of 5℃~16℃ for at least 384 hours; after cold soaking, allowing it to stand and separate into layers, removing the light impurities floating on the surface and the coarse particles precipitated at the bottom, and only taking the liquid of the naturally floating and settling substances in the middle layer, which is the shrimp shell cold soaking solution.

[0028] The specific preparation process of the *Cypressus chinensis* root bark suspension is as follows: Select *Cypressus chinensis* root bark free from mold and pests, and remove surface mud and impurities; under sterile conditions, cut into pieces, dry at low temperature, and pulverize into refined powder, pass through an 8-type sieve, place in a sealed container, add purified water, and cold soak at a low constant temperature of 5℃~16℃ for at least 384 hours. During the cold soaking process, the container must be kept sealed, and the mixture should be gently stirred once every 24 hours; after the cold soaking is completed, allow the liquid to stand to allow it to naturally separate into layers, remove the light impurities floating on the surface and the coarse particles precipitated at the bottom, and take the suspension slurry in the middle layer to obtain the *Cypressus chinensis* root bark suspension.

[0029] Step S2: Mix the prepared pomelo seed pulp, shrimp shell cold soaking liquid, and cypress root bark suspension in a stainless steel container lined with a plastic bag and kept sealed throughout the process. Add vitamin composition and potassium additive, and stir evenly at 5-16°C to obtain nutrient base solution. Step S3: Mix the nutrient base solution at a weight percentage of 32%–38% and water at 62%–68% in a stainless steel container lined with a plastic bag and kept sealed throughout the process to obtain the organic fertilizer suitable for plant foliar application.

[0030] This organic fertilizer can be used on a variety of crops, including Chinese cabbage, lettuce, red rapeseed, cabbage, pea shoots, wheat, green rapeseed, and lilyturf.

[0031] Example 1: This embodiment aims to verify the individual necessity and synergistic effects of the five core components of the organic fertilizer suitable for plant foliar application: pomelo seed pulp, shrimp shell cold soaking solution, cypress root bark suspension, vitamin composition, and potassium additive. By comparing crop growth indicators, yield, and quality (focusing on organic chromium content) of the "full component treatment" and the "single component deficiency treatment," the contribution of each component to fertilizer efficiency is clarified, providing experimental support for the rationality of the formulation.

[0032] Core validation indicators: crop yield, organic chromium content, stress resistance (leaf disease rate, lodging rate), and nutritional quality (chlorophyll content, fiber content / thousand-grain weight), ensuring that the experimental results correspond to the effects of existing technologies (10% increase in yield, 0.01~0.05 mg / kg of organic chromium).

[0033] The specific experimental procedure is as follows: (a) Experimental materials 1. Test crops: Three representative crops were selected (leaf vegetables: Chinese cabbage; grains: wheat; medicinal root and stem crops: Ophiopogon japonicus), all of which were local main varieties, and the seedlings grew in a uniform manner (Chinese cabbage with 4 leaves and 1 heart, wheat with 3 leaves and 1 heart, and Ophiopogon japonicus seedlings with a height of 8-10cm).

[0034] 2. Fertilizer composition: Prepare individual components and complete organic fertilizers according to existing technical solutions, ensuring that, except for the target missing component, the other raw materials and process parameters (low-temperature preparation, seasonal ratio) are completely consistent; the dilution water complies with GB 5749-2022 standard, and potassium humate (general type) is selected as the potassium element additive.

[0035] 3. Experimental equipment: atomizing sprayer (atomization effect 80%), electronic balance (accuracy 0.01g), chlorophyll analyzer, high performance liquid chromatograph (for detecting organic chromium), drying oven, etc.

[0036] 4. Experimental site: Select open field plots with uniform soil fertility and convenient irrigation and drainage, divide them into plots, and ensure that the previous crop is the same to avoid interference from residual soil nutrients; wheat and Chinese cabbage are replicated 3 times, and lilyturf is replicated 5 times (medicinal crops have higher stability requirements).

[0037] (II) Experimental Design Principles 1. Control Design: The nutrient deficiency control method was adopted. There were 6 treatment groups for each crop (1 full-component control group + 5 single-component deficiency groups). The area of ​​each plot was 15㎡ (5m long × 3m wide), the plot spacing was 1m, and isolation rows were set to prevent fertilizer drift.

[0038] 2. Seasonal adaptation: The experiment was conducted in spring (adapted to the growth period of most crops, corresponding to the spring formula). Chinese cabbage and wheat were formulated according to the spring formula, and Ophiopogon japonicus was formulated according to the spring nutrient base solution formula (1.5 parts potassium).

[0039] 3. Single variable: Only the addition of the target component is changed, while all other conditions (spraying time, frequency, dosage, environmental management) are completely uniform, strictly following existing technical usage specifications.

[0040] (III) Experimental treatment group setup Based on a spring-grown organic fertilizer (nutrient base solution 35% + water 65%, nutrient base solution ratio per 100 parts by weight: pomelo seed slurry 89 parts, shrimp shell cold soaking solution 1.8 parts, cypress root bark suspension 2.5 parts, vitamin composition 6 parts, potassium element additive 0.045 parts), the treatment groups were set up as follows:

[0041] Note: The principle for replenishing each missing group is "equal weight replacement", that is, using purified water to replenish the missing component by weight, ensuring that the total weight and dilution concentration of the nutrient base solution are consistent with the control group, and eliminating interference from concentration differences.

[0042] (iv) Experimental Implementation Steps 1. Sowing and transplanting Sowing and seedling raising were carried out according to conventional planting standards. After the seedlings reached the set growth level, they were transplanted into the experimental plots at the following density: 30 plants / m² for Chinese cabbage, 200 plants / m² for wheat, and 40 plants / m² for Ophiopogon japonicus. After transplanting, the seedlings were allowed to recover for 7 days (during which time they were watered with clean water and no fertilizer was applied).

[0043] 2. Foliar spraying operation (1) Spraying parameters: Follow the spring plan. The spraying amount for Chinese cabbage is 280L / hectare, wheat is 210L / hectare, and lilyturf is 234L / hectare. Spray from 7:00 to 8:00 every morning, once every 7 days, for a total of 2 sprays. Stop spraying 10 days before harvest.

[0044] (2) Operating procedures: Use a dedicated sprayer for each group. When spraying, cover both sides of the leaves evenly, and make sure the leaves are moist but not dripping. If it rains within 4 hours after spraying, spray the corresponding group of fertilizer solution (half the amount).

[0045] (3) Field management: During the experiment, weeding and irrigation were carried out uniformly (keeping the soil moisture at 60% to 70%). No other pesticides or fertilizers were applied. The occurrence of pests and diseases was recorded (only observed, without intervention, as an indicator of stress resistance).

[0046] 3. Sample collection and index testing (1) Sampling time: Chinese cabbage: 10 days after the last spraying (harvest period), 10 plants were randomly collected from each plot, and samples were taken from the middle and upper functional leaves and the whole plant.

[0047] Wheat: After the grain-filling period (7 days before harvest), 20 plants were randomly collected from each plot, and leaf and ear samples were taken; whole plants were collected at harvest time for yield measurement.

[0048] Ophiopogon japonicus: 20 days after the last spraying, 15 plants were randomly collected from each plot, and leaf and rhizome samples were taken.

[0049] (2) Detection indicators and methods: Yield indicators: For Chinese cabbage, the fresh weight of a single plant and the total yield of the plot are measured; for wheat, the thousand-grain weight and the yield per mu of the plot are measured; for Ophiopogon japonicus, the fresh weight of a single root and the total yield of the plot are measured.

[0050] Organic chromium content: The chromium content in the edible parts of crops (Chinese cabbage leaves, wheat grains, and Ophiopogon japonicus rhizomes) was determined by high performance liquid chromatography in accordance with GB 2762-2022 standard.

[0051] Nutritional and stress resistance indicators: chlorophyll content was measured using a chlorophyll meter; fiber content of Chinese cabbage was measured using the acid washing fiber method; lodging rate of wheat was calculated as the percentage of lodged plants in each plot; oleanolic acid content of Ophiopogon japonicus was measured using high performance liquid chromatography; leaf disease rate (number of diseased leaves / total number of leaves × 100%) was recorded for all crops.

[0052] (4) Data recording: Each indicator was tested three times, and the average value was taken. The difference rate between each treatment group and the control group (CK) was calculated.

[0053] 4. Experimental period Chinese cabbage: 45 days (from transplanting to harvest) Wheat: 60 days (from transplanting to the end of the grain-filling period) Ophiopogon japonicus: 90 days (from transplanting to the late stage of rhizome enlargement). (v) Data statistics and result judgment criteria 1. Data Statistics SPSS 26.0 software was used for data analysis. Difference tests were performed on the indicators between each treatment group and the control group (P<0.05 was considered significant, and P<0.01 was considered highly significant) to clarify the degree of influence of the missing components on the indicators.

[0054] 2. Criteria for Determining Necessity (1) Core essential components: After the absence of these components, the crop yield decreased by ≥5% compared to the control (corresponding to the existing 10% increase), and the organic chromium content decreased significantly (≤0.005mg / kg), or the difference in stress resistance / quality indicators was extremely significant (P<0.01), indicating that this component is the core of fertilizer effectiveness and cannot be replaced.

[0055] (2) Important and essential components: After the absence of these components, the crop yield decreased by 3% to 5% compared with the control, or the quality / stress resistance index showed significant differences (P<0.05), and the organic chromium content decreased slightly (0.005~0.01mg / kg), indicating that this component plays an important role in improving fertilizer efficiency.

[0056] (3) Synergistic essential components: After the absence of a single indicator, there is no significant difference, but multiple indicators decrease in combination, or the synergistic effect disappears (such as the synergistic enrichment effect of organic chromium and vitamin C), indicating that this component is the key to synergistic enhancement.

[0057] (vi) Expected experimental results and analysis of the necessity of components 1. CK group (all components): It meets the effects of existing technology, increases crop yield by 10% compared with conventional fertilization, has an organic chromium content of 0.02~0.03mg / kg, and has the best chlorophyll content and stress resistance.

[0058] 2. Group T1 (lacking pomelo seed pulp): The content of organic chromium decreased significantly (≤0.005mg / kg), and the yield decreased by ≥6%. It is a core essential component (the only source of plant-derived organic chromium).

[0059] 3. Group T2 (lacking shrimp shell cold soaking solution): Crop stress resistance is significantly reduced (disease rate increases by ≥8%), and wheat thousand-grain weight decreases by 3% to 4%. It is an important and necessary component (providing natural active substances and enhancing stress resistance).

[0060] 4. Group T3 (lacking *Symplocos lumbricoides* root bark suspension): The thickness of *Ophiopogon japonicus* rhizomes decreased, the content of oleanolic acid decreased by 4%–5%, and the fiber content of leafy vegetables increased, which is a necessary component for synergistic effects (improving medicinal quality and the taste of leafy vegetables).

[0061] 5. Group T4 (vitamin-deficient combination): Chlorophyll content decreased significantly (≥10%), the winter reserve effect of doubled vitamin C in autumn disappeared (the cold resistance of Ophiopogon japonicus decreased), and it is an important and necessary component (promoting nutrient absorption and stress resistance).

[0062] 6. T5 group (potassium-deficient additive): increased wheat lodging rate by ≥12%, insufficient root and stem enlargement of Ophiopogon japonicus, and decreased yield by 5% to 6%. It is a core essential component (regulates crop growth morphology and increases yield).

[0063] (vii) Precautions 1. The preparation of components must strictly follow the existing low-temperature process to avoid high temperature damage to active ingredients and ensure that the characteristics of each treatment group are consistent except for the target component.

[0064] 2. Strictly distinguish between different groups of sprayers during spraying to avoid cross-contamination; record meteorological data (temperature, precipitation) to eliminate the interference of environmental factors on the experimental results.

[0065] 3. When detecting organic chromium, a blank control (purified water) should be set up to ensure the accuracy of the test results and avoid interference from reagent and equipment residues.

[0066] 4. After the experiment, the data should be comprehensively analyzed, not only focusing on a single indicator, but also taking into account the synergistic effect, to fully determine the necessity of each component.

[0067] Example 2: This embodiment verifies the specific effects of using organic fertilizer on leafy vegetables, using cabbage as the experimental subject. The experimental field is as follows: Figure 1 As shown, the cabbage did not grow well and its leaves were yellowish-green when the organic fertilizer was not used.

[0068] The control group received conventional fertilization.

[0069] The nutrient base solution is prepared with a weight percentage of 35% nutrient solution and 65% water. The cabbage is planted in autumn. Therefore, the nutrient base solution components, by weight, are 75 parts pomelo seed slurry, 1.8 parts shrimp shell cold soaking solution, 3 parts cypress root bark suspension, 15 parts vitamin composition, and 0.04 parts potassium humate. The vitamin composition consists of 6 parts vitamin B2, 9 parts vitamin C, and 2 parts vitamin D3. An additional 9 parts of vitamin C will be added in autumn.

[0070] Choose a high-pressure sprayer with an atomization effect of ≥80%, and rinse it with clean water 3 times before use to avoid component conflicts caused by residual pesticides and fertilizers.

[0071] The specific process for using foliar fertilizer is as follows: 1. Spraying should be done between 6:00-8:00 or 18:00-20:00 every morning, avoiding the high temperature and strong sunlight period between 10:00-16:00; spraying is prohibited on cloudy or rainy days. If it rains within 4 hours after spraying, re-spraying is required, and the amount used for re-spraying is 50% of the amount used in the corresponding season.

[0072] 2. Operating standards: Spray evenly on both sides of the crop leaves, until the leaves are moist but not dripping (the amount of fertilizer solution adhering to a single leaf is about 0.5~1mL). For leafy vegetables, focus on spraying the middle and upper functional leaves.

[0073] 3. Frequency and cycle: Spray once every 7 to 10 days, and 2 to 3 times in a row constitute a complete application cycle. Stop spraying 10 days before harvest.

[0074] After one complete application cycle of this foliar fertilizer, the plant's growth is as follows: Figure 2 As shown, the cabbages are growing well, free from pests, and turning green.

[0075] Crop harvest: Compared with conventional fertilization, the yield is increased by 10%, the fresh weight of leaves increases by 8% to 12%, and the loss rate is reduced by 5%; the chlorophyll content is increased by 15%, and the fiber content is reduced by 7%; the organic chromium content is 0.02~0.03 mg / kg, which meets the safety standards.

[0076] Therefore, it can be seen that applying the foliar fertilizer provided by this invention to leafy vegetables can promote thick and tender leaves, enhance stress resistance, reduce losses during harvest, and adapt to the rapid growth characteristics of leafy vegetables.

[0077] Example 3: This embodiment verifies the specific effects of using organic fertilizer on medicinal root and rhizome crops, using *Ophiopogon japonicus* as the experimental subject. The condition of the *Ophiopogon japonicus* experimental field before using this organic fertilizer is as follows: Figure 3 As shown, the Ophiopogon japonicus seedlings are wilted and yellow, and their growth is poor. The roots, stems, and fruits of Ophiopogon japonicus are like... Figure 4 As shown, it produced almost no fruit, and the rhizomes were small.

[0078] The nutrient base solution is prepared with a weight percentage of 32% nutrient base solution and 68% water. The planting time for this Ophiopogon japonicus is autumn. Therefore, the nutrient base solution components, by weight, are: 78 parts pomelo seed pulp, 2.0 parts shrimp shell cold soaking solution, 3.5 parts cypress root bark suspension, 18 parts vitamin composition, and 0.06 parts potassium humate. The vitamin composition consists of 6.5 parts vitamin B2, 9.5 parts vitamin C, and 2.5 parts vitamin D3. An additional 9.5 parts vitamin C will be added in autumn.

[0079] Choose a high-pressure sprayer with an atomization effect of ≥80%, and rinse it with clean water 3 times before use to avoid component conflicts caused by residual pesticides and fertilizers.

[0080] The specific process for using foliar fertilizer is as follows: 1. Spray three times consecutively, with a 10-day interval between each application. Stop spraying 20 days before harvest.

[0081] 2. When spraying, lightly spray the soil surface around the roots, ensuring the soil is moistened to a depth of ≤2cm to promote root and stem absorption; during periods of low temperature, spray after 9:00 AM to avoid frost damage.

[0082] After one complete application cycle of this foliar fertilizer, the condition of the experimental field was as follows: Figure 5 As shown, the Ophiopogon japonicus seedlings have turned green and are upright, showing a significant improvement in seedling growth. The specific growth of Ophiopogon japonicus is as follows: Figure 6 As shown, the Ophiopogon japonicus begins to produce fruit, which is white in color.

[0083] Crop harvest: The thickness of the rhizome increased by 12%, the yield per mu increased by 10%, and the content of oleanolic acid (the main active ingredient of Ophiopogon japonicus) increased by 8%; the organic chromium content of the rhizome was 0.015 mg / kg, which met the safety standards for medicinal crops and had no heavy metal residues.

[0084] Therefore, it can be seen that using this organic fertilizer can promote root and stem enlargement, increase the content of effective ingredients (oleanolic acid), and enhance the ability to withstand winter cold.

[0085] Example 4: This embodiment verifies the specific effects of using organic fertilizer on grain crops, with wheat as the experimental subject.

[0086] The nutrient base solution was prepared with a weight percentage of 38% nutrient solution and 62% water. The wheat was planted in autumn. Therefore, the nutrient base solution components, by weight, were 74 parts pomelo seed slurry, 1.5 parts shrimp shell cold soaking solution, 2.5 parts cypress root bark suspension, 16 parts vitamin composition, and 0.03 parts potassium humate. The vitamin composition consisted of 5.5 parts vitamin B2, 8.5 parts vitamin C, and 1.5 parts vitamin D3. An additional 8.5 parts vitamin C were added in autumn.

[0087] Choose a high-pressure sprayer with an atomization effect of ≥80%, and rinse it with clean water 3 times before use to avoid component conflicts caused by residual pesticides and fertilizers.

[0088] The specific process for using foliar fertilizer is as follows: 1. Spray three times consecutively, with a 10-day interval between each application. Stop spraying 20 days before harvest.

[0089] 2. When spraying, aim at the upper and middle leaves and ears of wheat, avoiding midday during the flowering period to prevent pollen loss; suspend spraying in windy weather to avoid fertilizer drift.

[0090] After one complete application cycle of this foliar fertilizer, the condition of the experimental field was as follows: Figure 7 As shown, the seedling growth effect is significant, with an average increase in plant height of about 5cm.

[0091] Crop harvest: 1,000-grain weight increased by 9%, yield per mu increased by 10%, and lodging resistance was significantly enhanced; the organic chromium content in the grains was 0.012 mg / kg, far below the safety threshold, meeting food safety standards.

[0092] It is evident that using this organic fertilizer promotes stem and leaf growth, enhances grain plumpness during the grain-filling period, and improves lodging resistance and thousand-grain weight.

[0093] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A plant foliar suitable organic fertilizer, characterized in that, It is prepared by mixing nutrient base solution and water in weight percentage, wherein the nutrient base solution accounts for 32% to 38% and the water accounts for 62% to 68%; the nutrient base solution includes pomelo seed pulp, shrimp shell cold soaking solution, cypress root bark suspension, vitamin composition and potassium element additive.

2. The organic fertilizer suitable for plant foliar application according to claim 1, characterized in that, The nutrient base solution has different proportions depending on the season, by weight. Spring: 88-90 parts pomelo seed pulp, 1.5-2.0 parts shrimp shell cold soaking solution, 2.0-3.0 parts cypress root bark suspension, 5-7 parts vitamin composition, and 0.03-0.06 parts potassium element additive; Summer: 78-83 parts pomelo seed pulp, 1.5-2.0 parts shrimp shell cold soaking solution, 3.0-4.0 parts cypress root bark suspension, 12-14 parts vitamin composition, and 0.03-0.06 parts potassium element additive; Autumn: 74-78 parts of pomelo seed pulp, 1.5-2.0 parts of shrimp shell cold soaking solution, 2.5-3.5 parts of cypress root bark suspension, 16-18 parts of vitamin composition, and 0.03-0.06 parts of potassium element additive.

3. The organic fertilizer suitable for plant foliar application according to claim 2, characterized in that, The vitamin composition consists of vitamin B2, vitamin C, and vitamin D3, with each vitamin in a seasonal ratio, by weight. Springtime: Vitamin B2 1.5–2.5 parts, Vitamin C 2.5–3.5 parts, Vitamin D3 0.5–1.5 parts; Summer: Vitamin B2 3.5-4.5 parts, Vitamin C 5.5-6.5 parts, Vitamin D3 2.5-3.5 parts; Autumn: Vitamin B2 5.5-6.5 parts, Vitamin C 8.5-9.5 parts, Vitamin D3 1.5-2.5 parts.

4. A foliar organic fertilizer for plants according to claim 2 or 3, characterized in that, The potassium element additive is at least one of potassium humate, potassium citrate, and wood ash leachate. The dosage of the potassium element additive varies with the season and also needs to be adjusted according to the differences in plant parts. By weight, the dosage of potassium element additive is 1.5 parts for root and rhizome plants, 1.8 parts for leaf and flower plants, and 2.3 parts for fruit and seed plants.

5. A method for preparing a foliar organic fertilizer for plants according to any one of claims 1 to 4, characterized in that, Includes the following steps: Step S1: Prepare pomelo seed pulp, shrimp shell cold soaking solution, and cypress root bark suspension respectively; Step S2: Mix the prepared pomelo seed pulp, shrimp shell cold soaking liquid, and cypress root bark suspension, add vitamin composition and potassium element additive, and stir evenly at 5-16℃ to obtain nutrient base solution. Step S3: Mix the nutrient base solution at a weight percentage of 32%–38% and water at a weight percentage of 62%–68% to obtain the organic fertilizer suitable for plant foliar application.

6. The method for preparing a plant foliar organic fertilizer according to claim 5, characterized in that, The specific preparation process of the pomelo seed pulp in step S1 is as follows: Take 200 kg of at least one of pomelo, grapefruit or grapefruit as raw material, use 20 kg of water and 200 g of baking soda to prepare a cleaning solution, clean the surface of the raw material, and then air dry it naturally. Grind it in a low temperature environment of 5℃~16℃; add 6 kg of sucrose to the ground raw material, seal it in a container and ferment it in a low temperature environment of 5℃~16℃ for at least 384 hours. During the fermentation process, the environment should be kept away from light and the pressure inside the container should be maintained at normal pressure. Filter to obtain pomelo seed pulp.

7. The method for preparing a foliar organic fertilizer according to claim 5, characterized in that, The specific preparation process of the shrimp shell cold soaking solution in step S1 is as follows: using clean crayfish shells as raw materials, soaking them in purified water for 2 hours, then cutting them into 0.5cm-1cm fragments with a knife or crusher, and air-drying them in a ventilated and dry environment, or drying them at a low temperature below 45℃, until the shrimp shell moisture content is ≤5%; then putting the dried shrimp shells into an ultra-fine pulverizer to pulverize them, passing them through an 8-type sieve to obtain extremely fine shrimp shell powder; adding the extremely fine shrimp shell powder to purified water, placing it in a sealed container, and cold soaking it at a low temperature of 5℃~16℃ for at least 384 hours; after cold soaking, allowing it to stand and separate into layers, removing the light impurities floating on the surface and the coarse particles precipitated at the bottom, and only taking the liquid of the naturally floating and settling substances in the middle layer, thus obtaining the shrimp shell cold soaking solution.

8. The method for preparing a foliar organic fertilizer for plants according to claim 5, characterized in that, The specific preparation process of the *Cypressus chinensis* root bark suspension in step S1 is as follows: Select *Cypressus chinensis* root bark free from mold and pests, and remove surface mud and impurities; under sterile conditions, cut into pieces, dry at low temperature, and pulverize into refined powder, pass through an 8-type sieve, place in a sealed container, add purified water, and cold soak at a low temperature of 5℃~16℃ for at least 384 hours. During the cold soaking process, the container must be kept sealed, and the mixture should be gently stirred once every 24 hours; after the cold soaking is completed, let it stand to allow the liquid to naturally separate into layers, remove the light impurities floating on the surface and the coarse particles precipitated at the bottom, and take the suspension slurry in the middle layer to obtain the *Cypressus chinensis* root bark suspension.

9. A method for preparing a plant foliar organic fertilizer according to any one of claims 6 to 8, characterized in that, The mixing process in steps S2 and S3 is carried out in a stainless steel container lined with a plastic bag and kept sealed throughout the process.

10. A method for applying a foliar organic fertilizer to plants according to any one of claims 1 to 4, characterized in that, Includes the following steps: (1) According to the season, the organic fertilizer is prepared. The basic spraying amount of organic fertilizer is 150~200L / hectare. In response to the growth needs of plant roots, stems, leaves, flowers, fruits and seeds, the potassium element additive is adjusted according to the weight ratio of 1.5 parts for roots and stems, 1.8 parts for leaves and flowers, and 2.3 parts for fruits and seeds. At the same time, vitamin B2, vitamin C and vitamin D3 are added according to the seasonal ratio. (2) In spring, use the basic amount of organic fertilizer for spraying; in summer, increase the amount by 10% on the basis of the basic amount of fertilizer; in autumn, reduce the amount by 10% on the basis of the basic amount of fertilizer; when spraying in autumn, double the proportion of vitamin C in the vitamin composition. (3) Use a misting sprayer and spray at 6:00-8:00 or 18:00-20:00 every morning, avoiding high temperature and strong light periods and rainy days; if it rains within 4 hours after spraying, re-spraying is required, and the amount of organic fertilizer used should be halved when re-spraying; (4) Spray once every 7 to 10 days, and spray 2 to 3 times in a row for one growth cycle.

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