Plant-source high-palatability bait matched with trapping and killing box or monitoring device for use and used for luring termites to gather

Abstract

The invention discloses plant-source high-palatability bait matched with a trapping and killing box or a monitoring device for use and used for luring termites to gather, and belongs to the technical field of termite prevention and control. According to the invention, through scientific compounding of the plant source food calling pheromone (tea polyphenol) and the synthetic aggregation pheromone (cis-3, cis-6, trans-8-dodecatriene-1-alcohol), the whole process guidance of the foraging behavior of the termites is realized; plant extracts (tea polyphenol, pine wood extract and fungus extract) are adopted as core attractants, so that the dependence on complete synthesis of pheromones is reduced; according to the bait provided by the invention, the termites in a region are successfully, rapidly and continuously attracted and gathered to a controllable point location, and the termite groups are efficiently cleared in cooperation with low-dose precise pesticide application subsequently, and no diffusion pollution is caused to the environment.

Description

Technical Field

[0001] This invention belongs to the field of termite control technology, and in particular relates to a plant-based, highly palatable bait for attracting termites to gather, used in conjunction with a bait box or monitoring device. Background Technology

[0002] Termites are a serious social pest that can cause enormous economic losses by damaging buildings, water conservancy dams, agricultural and forestry crops, and ancient trees in gardens. Traditional chemical barrier control methods suffer from problems such as environmental pollution, pesticide residues, and the development of pesticide resistance. Therefore, integrated monitoring and control technology with bait systems at its core has become an important direction for modern integrated termite management (IPM) due to its high efficiency, environmental friendliness, and strong targeting.

[0003] The core of this technology lies in utilizing the foraging behavior of termites to lure them into a specific device using bait, thereby enabling concentrated extermination or monitoring of the population dynamics. However, existing baits still have significant shortcomings in terms of effectiveness and application. First, many baits rely solely on natural materials that termites prefer (such as sugarcane bagasse, pine sawdust, and sugars) as basic attractants. In environments where there are many similar competing foods in nature, the attraction efficiency is low and the speed is slow, failing to achieve rapid and precise targeted attraction. Second, while some technologies attempt to introduce pheromones, they are either limited to single-component feeding pheromones, failing to effectively induce termites to gather in the device for extended periods and summon companions, resulting in termites coming but not staying, leading to low control efficiency; or they use pheromones extracted from live termites, whose activity is difficult to sustain. Furthermore, while some advanced biological control solutions are highly effective, they typically rely on complex specialized production equipment and intelligent release devices, resulting in high system costs and difficulty in directly adapting to widely used standardized and simple monitoring / trapping boxes (piles), limiting their flexibility for large-scale promotion and application.

[0004] Therefore, developing a highly efficient bait that is effective in attracting and gathering termites, is environmentally friendly, and can be used in conjunction with standard bait boxes or monitoring devices is of great significance for improving the practicality and economic benefits of existing termite control technologies. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a highly palatable plant-based bait for attracting termites to their nests, used in conjunction with bait boxes or monitoring devices. This highly efficient bait, with plant-derived extracts as its core attractant ingredient and compatible with standard bait boxes or monitoring devices, achieves rapid attraction and nest aggregation of termites through a synergistic effect of both attracting food and promoting aggregation.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a plant-based, highly palatable bait for attracting termites to gather, used in conjunction with bait boxes or monitoring devices, comprising the following raw materials in parts by weight: 1-50 parts of cis-3, cis-6, trans-8-dodecathorien-1-ol, 1-50 parts of tea polyphenols, 10-100 parts of microcrystalline cellulose, 1-50 parts of maltose, 1-100 parts of glucose, 1-50 parts of sucrose, 10-1000 parts of fermented sugarcane bagasse powder, 50-1000 parts of lignocellulose powder, 0-50 parts of pine wood extract, 1-50 parts of potassium sorbate, 1-100 parts of fungal extract, 50-1000 parts of acetone, 1-50 parts of termite nest fungal powder, and 50-1000 parts of binder.

[0007] The technical principles of this invention and the functions of each component: 1. Pheromone system: (1) Choosing cis-3, cis-6, trans-8-dodecanetrien-1-ol as the aggregation pheromone, which is the main component of the tracer pheromone released by termites (especially the genera *Dendrocalamus* and *Reticulitermes*) and worker termites after discovering a high-quality food source, this invention can strongly simulate the above signal by directly adding cis-3, cis-6, trans-8-dodecanetrien-1-ol, guiding subsequent termites to gather along the path to the bait device, and inhibiting them from leaving the site too early, thus creating the illusion of a food source outside the nest; (2) Tea polyphenols are selected as plant-derived feeding pheromones. Tea polyphenols and other plant polyphenols have been proven to have significant attraction and feeding-promoting effects on termites. Their odor spectrum is similar to the signaling substances produced by the decomposition of plant tissues that termites naturally feed on. This invention uses them as the main feeding ingredient, providing initial attraction over long distances. They are also natural, cost-controllable, and environmentally friendly. The combination of tea polyphenols and aggregation pheromones forms a complete behavioral guidance chain from long-distance attraction to close-range aggregation and positioning.

[0008] 2. Nutritional and substrate basis: (1) Sugar combination (maltose, glucose, sucrose): provides a rapid energy source, meets the termites' demand for carbohydrates, and significantly improves the initial feeding rate (palmistry) of the bait. (2) Cellulose combination (microcrystalline cellulose, lignocellulose powder, fermented bagasse powder): constitutes the main skeleton and main carbon source of bait. Microcrystalline cellulose provides uniform texture, lignocellulose powder simulates wood structure, and fermented bagasse powder not only provides the fiber that termites prefer, but the organic acids and alcohols produced during its fermentation process also have an additional attraction effect. (3) Termite nest fungal powder: contains the metabolic products of symbiotic microorganisms of termites and the unique odor substances in the nest, which can greatly reduce the vigilance of termites, create a safe and nest-like feeding environment, and effectively promote their long-term and large-scale gathering for feeding.

[0009] 3. Efficiency Enhancement and Stability Module: (1) Pine wood extract: Pine wood extract is mainly composed of terpenoids, which are the main components of pine resin. It has a strong attraction to Formosan subterranean termites and Reticulitermes, and can produce odor synergy with aggregation pheromones to enhance the overall attraction effect. (2) Fungal extract: Termites have the habit of cultivating fungi as food. By adding fungal extract to simulate the smell of their fungal garden, the termites can be further attracted and soothed. (3) Potassium sorbate: As a safe food preservative, it can prevent bait from becoming moldy in a humid environment and maintain the freshness and effectiveness of bait in the wild for several weeks; (4) Acetone: As an excellent organic solvent, it is used to uniformly dissolve and disperse trace amounts of highly active aggregated pheromone components into the entire bait matrix, ensuring its stability and slow release; (5) Adhesive: Provides the adhesive force required for molding, so that the bait can be made into a fixed shape, which is convenient for installation in the device and can maintain the structure under certain humidity, which is conducive to termite feeding and does not collapse too quickly.

[0010] Furthermore, the lignocellulose powder is selected from at least one of Masson pine powder, eucalyptus powder, and sweetgum powder.

[0011] Furthermore, the pine wood extract is selected from at least one of longifolene, cedrol, and myrtol.

[0012] Furthermore, the fungal extract is selected from at least one of Ganoderma lucidum extract, Ganoderma sinense extract, and Poria cocos extract.

[0013] Furthermore, the termite nest fungal powder is a powder obtained by drying, sterilizing and pulverizing termite nests.

[0014] Furthermore, the preparation method of the fermented sugarcane bagasse powder includes: crushing the sugarcane bagasse to 100 mesh, adjusting the moisture content to 60-70%, then naturally fermenting it at 26-30℃ for 5-12 days, drying it to a moisture content ≤10%, crushing it again and passing it through an 80 mesh sieve to obtain the fermented sugarcane bagasse powder.

[0015] This invention provides a method for preparing a highly palatable plant-based bait for attracting termites, as described above, in conjunction with a bait box or monitoring device, comprising the following steps: (1) Mix microcrystalline cellulose, maltose, glucose, sucrose, fermented sugarcane bagasse powder, lignocellulose powder, pine wood extract, potassium sorbate, fungal extract and termite nest fungal nursery powder to obtain dry mixed base material; (2) Dissolve cis-3, cis-6, trans-8-dodecathien-1-ol and tea polyphenols in a portion of acetone to obtain a pheromone solution; add the pheromone solution to the dry-mixed base material to obtain a mixture. (3) Mix the adhesive with the remaining acetone and water to obtain an adhesive solution; mix the adhesive solution with the mixture obtained in step (2), and after molding and drying, obtain the plant-based highly palatable bait for attracting termites to gather, which is used in conjunction with the bait box or monitoring device.

[0016] Furthermore, in step (2), the pheromone solution is added by spraying.

[0017] Further, in step (3), the drying process involves drying the bait at 40-50°C until the moisture content is 5-10%.

[0018] The present invention also provides a termite control method, which involves placing the highly palatable plant-based bait used in conjunction with a bait box or monitoring device to attract and gather termites in the termite monitoring device or bait box.

[0019] Compared with the prior art, the present invention has the following advantages and technical effects: 1. Significantly enhanced attraction and aggregation efficiency: Through the scientific combination of plant-derived feeding pheromones (tea polyphenols) and synthetic aggregation pheromones (cis-3, cis-6, trans-8-dodecanetrien-1-ol), the entire process of termite foraging behavior was guided. The results showed that the bait of this invention reduced the time to first attract termites in the field monitoring device by more than 50% compared with traditional sugarcane bagasse sugar bait. During the observation period of 7-15 days, the biomass (weight) of termites in the device could reach 3-8 times that of the control group, truly achieving rapid and large-scale aggregation.

[0020] 2. High device adaptability and ease of operation: The bait of this invention is designed in a uniform solid form (block, strip, flake) with moderate physical strength. It can be directly placed into any standardized bait box or monitoring device without modification, which greatly facilitates the deployment work of front-line prevention and control personnel and improves the popularization and applicability of the technology.

[0021] 3. Green, environmentally friendly and highly safe: The core attractant uses a large amount of plant extracts (tea polyphenols, pine wood extracts, fungal extracts), reducing the dependence on fully synthetic pheromones. The bait does not contain any acute killing ingredients; its function is only to gather the insects. The final killing can be achieved by releasing low doses of insect growth regulators or pathogenic microorganisms after the gathering is successful. This minimizes the total amount of chemical pesticides that directly enter the environment, making it environmentally friendly and safe for humans and animals.

[0022] 4. Significantly reduced control costs and increased efficiency: Due to its extremely high aggregation efficiency, insecticides can be applied to highly concentrated groups within a very small device space, increasing insecticide utilization by more than 70% and avoiding large-scale waste from traditional spraying or dusting. At the same time, rapid group control shortens the control cycle, reduces the number of manual checks and re-applications, and significantly reduces overall costs.

[0023] 5. Delaying the development of drug resistance: This invention divides the attraction and gathering into two relatively independent stages. No pesticides are used during the gathering stage, which avoids the behavior avoidance or physiological resistance of the colony to pesticides during the foraging process. After the termites have fully adapted and gathered in large numbers on the bait, pesticides (such as chitin synthesis inhibitors) are used to achieve precise strikes and greatly reduce the selective pressure of resistance.

[0024] 6. Long shelf life and good stability: Through scientific formulation (such as using potassium sorbate for mold prevention) and preparation process (slow release of pheromone solution by spraying and embedding), the activity of key pheromone components is maintained in the bait during storage and the initial stage of field use. The shelf life can reach 12 months under sealed conditions at room temperature. Experimental results show that the bait can remain effective in field devices for 4-8 weeks. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to specific embodiments.

[0027] This invention provides a highly palatable plant-based bait for attracting termites, used in conjunction with a bait box or monitoring device. The bait comprises the following ingredients in parts by weight: 1-50 parts cis-3,cis-6,trans-8-dodecathorien-1-ol, 1-50 parts tea polyphenols, 10-100 parts microcrystalline cellulose, 1-50 parts maltose, 1-100 parts glucose, 1-50 parts sucrose, 10-1000 parts fermented sugarcane bagasse powder, 50-1000 parts lignocellulose powder, 0-50 parts pine wood extract, 1-50 parts potassium sorbate, 1-100 parts fungal extract, 50-1000 parts acetone, and white... The mixture comprises 1-50 parts of termite nest fungal powder and 50-1000 parts of binder; more preferably: 1-3 parts of cis-3, cis-6, trans-8-dodecathorien-1-ol, 5-30 parts of tea polyphenols, 30-80 parts of microcrystalline cellulose, 5-30 parts of maltose, 10-60 parts of glucose, 5-30 parts of sucrose, 300-800 parts of fermented sugarcane bagasse powder, 150-740 parts of lignocellulose powder, 0-30 parts of pine wood extract, 5-20 parts of potassium sorbate, 10-60 parts of fungal extract, 200-600 parts of acetone, 10-25 parts of termite nest fungal powder and 300-500 parts of binder.

[0028] In a preferred embodiment, the microcrystalline cellulose is in the form of powder, granules, or ultrafine powder, and is more preferably in the form of powder microcrystalline cellulose.

[0029] In a preferred embodiment, the lignocellulose powder is selected from at least one of Masson pine powder, eucalyptus powder and sweetgum powder, and is more preferably eucalyptus powder.

[0030] In a preferred embodiment, the pine wood extract is selected from at least one of longifene, cedrol and myrtol, and more preferably longifene.

[0031] In a preferred embodiment, the fungal extract is selected from at least one of Ganoderma lucidum extract, Ganoderma sinense extract and Poria cocos extract, and is more preferably Poria cocos extract.

[0032] In a preferred embodiment, the termite nest fungal powder is a powder obtained by drying, sterilizing and pulverizing termite nests; the sterilization temperature is 60-80℃ and the sterilization time is 3 hours; the pulverization process also includes a sieving step; the sieving is through an 80-mesh sieve.

[0033] In a preferred embodiment, the preparation method of the fermented sugarcane bagasse powder includes: crushing sugarcane bagasse to 100 mesh, adjusting the moisture content to 60-70%, and naturally fermenting it at 26-30℃ for 5-12 days, thereby degrading some fibers through microbial metabolism and producing odorous substances such as organic acids and alcohols that termites prefer; then drying it to a moisture content ≤10%, crushing it again, and passing it through an 80-mesh sieve to obtain the fermented sugarcane bagasse powder.

[0034] In a preferred embodiment, the binder is selected from agar and / or carrageenan.

[0035] This invention provides a method for preparing a highly palatable plant-based bait for attracting termites, as described above, in conjunction with a bait box or monitoring device, comprising the following steps: (1) Mix microcrystalline cellulose, maltose, glucose, sucrose, fermented sugarcane bagasse powder, lignocellulose powder, pine wood extract, potassium sorbate, fungal extract and termite nest fungal nursery powder to obtain dry mixed base material; (2) Dissolve cis-3, cis-6, trans-8-dodecathien-1-ol and tea polyphenols in a portion of acetone to obtain a pheromone solution; add the pheromone solution to the dry-mixed base material to obtain a mixture. (3) Mix the adhesive with the remaining acetone and water to obtain an adhesive solution; mix the adhesive solution with the mixture obtained in step (2), and after molding and drying, obtain the plant-based highly palatable bait for attracting termites to gather, which is used in conjunction with the bait box or monitoring device.

[0036] In a preferred embodiment, in step (2), the portion of acetone is 50-60% of the total amount of acetone used.

[0037] In a preferred embodiment, in step (2), the pheromone solution is added by spraying.

[0038] In a preferred embodiment, in step (3), the temperature of the adhesive solution is 55°C.

[0039] In a preferred embodiment, in step (3), the molding process is as follows: the mixed wet material is filled into a mold and pressed into a square sheet of 20g / piece, or extruded into a cylindrical strip with a diameter of 8mm, or extruded into a sheet with a length of 5cm, a width of 5cm, and a thickness of 1cm.

[0040] In a preferred embodiment, in step (3), the drying process involves drying the bait at 40-50°C until the moisture content is 5-10%.

[0041] The present invention also provides a termite control method, which involves placing the highly palatable plant-based bait used in conjunction with a bait box or monitoring device to attract and gather termites in the termite monitoring device or bait box.

[0042] Unless otherwise specified, "parts" in the embodiments of this invention represent "number of parts by weight".

[0043] Unless otherwise specified, all raw materials used in the embodiments of this invention were purchased through commercial channels.

[0044] In the following examples, the Ganoderma lucidum extract powder, Ganoderma lucidum extract powder, and Poria cocos extract powder were all purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., and the content of each extract was ≥95%. Both longleaf ethoxylate and cedrol were purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., and the content of each extract was ≥65%. The tea polyphenols were purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., and are green tea extracts with a tea polyphenol content of ≥98%.

[0045] Example 1 A plant-based, highly palatable bait (with moderate attractant efficacy) for use with bait boxes or monitoring devices to attract termites, is composed of the following ingredients: 1 part cis-3,cis-6,trans-8-dodecathorien-1-ol, 15 parts tea polyphenols, 50 parts powdered microcrystalline cellulose, 15 parts maltose, 30 parts glucose, 15 parts sucrose, 300 parts fermented sugarcane bagasse powder, 300 parts Masson pine wood powder, 15 parts longifolene, 10 parts potassium sorbate, 30 parts Poria cocos extract powder, 400 parts acetone, 10 parts termite nest fungal powder, and 300 parts a mixture of agar and carrageenan (1:1). The preparation method of fermented sugarcane bagasse powder is as follows: sugarcane bagasse is crushed to 100 mesh, the moisture content is adjusted to 60%, and it is naturally fermented at 26℃ for 10 days. After that, it is dried to a moisture content of ≤10%, crushed again and passed through an 80 mesh sieve to obtain fermented sugarcane bagasse powder. The preparation method of termite nest fungal powder is as follows: after the dried termite waste nest is sterilized at 80℃ for 3 hours, it is crushed and passed through an 80-mesh sieve to obtain termite nest fungal powder. The preparation method of the above-mentioned plant-based highly palatable bait for attracting termites to gather, used in conjunction with bait boxes or monitoring devices, includes the following specific steps: (1) Powdered microcrystalline cellulose, maltose, glucose, sucrose, fermented sugarcane bagasse powder, Masson pine wood powder, longleaf cellulose, potassium sorbate, Poria cocos extract powder and termite nest fungal powder are put into a mixing mixer and mixed for 45 minutes to obtain dry mixed base material. (2) Dissolve cis-3, cis-6, trans-8-dodecanetrien-1-ol and tea polyphenols in 200 parts of acetone to obtain a pheromone solution; under stirring, add the obtained pheromone solution evenly to the dry mixed base material obtained in step (1) by spraying to obtain a mixed material. (3) Dissolve agar and carrageenan in the remaining 200 parts acetone and 600 parts hot water, heat and stir until completely dissolved to obtain an adhesive solution at a temperature of about 55°C; mix the obtained adhesive solution and the mixture obtained in step (2) thoroughly in a kneader to form a wet material with good plasticity; extrude the obtained wet material into a sheet with a length of 5cm, a width of 5cm and a thickness of 1cm, and then dry it at 45°C until the moisture content is ≤10%, and seal it in a package.

[0046] Field Implementation Results: The flake bait (8g / piece) prepared in Example 1 was placed into standard bait boxes, with 2 pieces of flake bait in each box. Ten bait boxes were placed in the same area in five different locations (urban park (Area 1), hospital green space (Area 2), reservoir dam (Area 3), green belt (Area 4), and arboretum (Area 5)). Ten control group bait boxes (containing equal amounts of common commercially available termite bait, mainly composed of sugarcane bagasse, pine sawdust, sugar, and 0.25% flufenoxuron) were also placed in each area. The distance between bait boxes of the same treatment in the same area was greater than 50 meters, while the distance between bait boxes in the control and treatment groups was 5-8 meters. The results were checked on day 7 and day 14, and are shown in Tables 1-3. Meanwhile, observations on day 7 revealed that worker Formosan subterranean termites had been attracted to feed. Within 15 days, the average number of termites gathered in the treatment group's bait box was 15.08 grams (biomass), while the average number of termites gathered in the control group's bait box was 8.08 grams (biomass).

[0047] Table 1. Average attraction rates on days 7 and 14. Table 2. Analysis of differences in average attraction rates Note: Numerical values ​​are expressed as mean ± standard deviation (n=5). Paired-samples t-tests were used for comparisons between groups, with a significance level set at p<0.05. Different lowercase letters (a and b, c and d) in the same column indicate significant differences between the treatment group and the control group at the same time point.

[0048] As shown in Table 2, the termite attraction rate using the bait (treatment group) of Example 1 of the present invention was significantly higher than that of the control group in both the 7-day and 14-day test periods (p<0.05).

[0049] Table 3 Average feed consumption on day 14 Table 4. Analysis of the differences in average feed consumption Note: Data are expressed as mean ± standard deviation (n=5). Paired-samples t-tests were used for comparisons between groups, with a significance level set at p<0.05. Different lowercase letters (a and b) in the same column indicate significant differences in average feed consumption between the treatment group and the control group.

[0050] During a 14-day field experiment, the results of the bait consumption measurement showed that the average consumption of the bait in Example 1 of this invention was as high as 11.28±1.55g, significantly higher than the average consumption of 3.55±1.35g of the control group bait. Specifically, the consumption of the bait in Example 1 of this invention was 3.18 times that of the control group, with an average consumption of 7.73 grams more. The data from Example 1 directly and powerfully confirm, from the final result of feeding behavior, that the bait of this invention has an extremely significant effect on improving termite palatability, promoting termite aggregation, and prolonging their feeding time. The significant difference in the consumption of the bait of this invention, together with the aforementioned attraction rate data, illustrates that this invention achieves comprehensive and efficient guidance of termite foraging behavior through a pheromone synergy strategy.

[0051] Example 2 A highly palatable plant-based bait (with extremely strong attractant effect) for use with bait boxes or monitoring devices to attract termites, suitable for areas in the early stages of termite activity or with low population density, is composed of the following ingredients: 3 parts cis-3, cis-6, trans-8-dodecanetrien-1-ol, 30 parts tea polyphenols, 30 parts ultra-fine powdered microcrystalline cellulose, 30 parts maltose, 60 parts glucose, 30 parts sucrose, 600 parts fermented sugarcane bagasse powder, 150 parts Liquidambar formosana powder, 30 parts cedrol, 5 parts potassium sorbate, 60 parts Ganoderma lucidum extract powder, 600 parts acetone, 25 parts termite nest fungal garden powder, and 500 parts carrageenan. The preparation methods for fermented sugarcane bagasse powder and termite nest fungal nursery powder are the same as in Example 1; The method for preparing plant-derived highly palatable bait for attracting termites to gather, used in conjunction with bait boxes or monitoring devices, differs from Example 1 only in that, in step (3), the obtained wet material is filled into a mold and pressed into a block of 20g / block, then dried at 45°C until the moisture content is ≤10%, and then sealed and packaged.

[0052] Field Implementation Results: The block bait from Example 2 was placed inside the monitoring devices. Ten monitoring devices in three different outdoor locations (urban park (Area 1), hospital green space (Area 2), and reservoir dam (Area 3)) with ant infestations were selected as the treatment group. One block of bait was placed in each monitoring device in the treatment group. Ten monitoring devices were also selected in each area for the control group without any treatment. The distance between the monitoring devices in the control and treatment groups was 5-10 meters. Observations were conducted on days 4, 7, and 10, and the results are shown in Tables 5-6.

[0053] Table 5. Average attraction rates on days 4, 7, and 10. Table 6. Analysis of differences in average attraction rates Note: Data are expressed as mean ± standard deviation (n=3). Paired-samples t-tests were used for comparisons between groups. The significance level was set at p<0.05. Different lowercase letters in the same column (a and b, c and d, e and f) indicate the same time point. Significant differences were found between the treatment group and the control group.

[0054] The results showed that, due to the high concentration of pheromones and sugars in the bait of Example 2, the attraction speed was fast. The bait was discovered by termites and established a feeding path within 2-4 days outdoors. It also had a significant effect on the summoning of soldier ants and could quickly attract the feeding group.

[0055] Example 3 A highly palatable plant-based bait for attracting termites, used in conjunction with bait boxes or monitoring devices, suitable for large-scale deployment and long-term monitoring, is composed of the following ingredients: 1 part cis-3,cis-6,trans-8-dodecathorien-1-ol, 5 parts tea polyphenols, 80 parts granular microcrystalline cellulose, 5 parts maltose, 10 parts glucose, 5 parts sucrose, 800 parts fermented sugarcane bagasse powder, 700 parts lignocellulose powder, 40 parts eucalyptus powder, 20 parts potassium sorbate, 10 parts Ganoderma lucidum extract powder, 200 parts acetone, 10 parts termite nest fungal garden powder, and 400 parts agar; wherein, eucalyptus powder is used to provide an attraction effect similar to that of pine wood extract. The preparation methods for fermented sugarcane bagasse powder, termite nest fungal garden powder, and plant-derived highly palatable bait used in conjunction with bait boxes or monitoring devices to attract termites are the same as in Example 1.

[0056] Field application results: Due to the high content of microcrystalline cellulose and the large amount of potassium sorbate, the cost of bait in Example 3 was significantly reduced. At the same time, it had strong resistance to mold growth in moist soil in the field, and the effective duration could be extended to 8-10 weeks. The initial attraction speed was slower than that in Example 1, but it still showed a stable aggregation effect.

[0057] Example 4 (Comparative experiment to verify the synergistic effect of pheromones) Experimental group: Feed from Example 1; Control group A: The only difference from the bait in Example 1 is that it does not contain cis-3, cis-6, trans-8-dodecanetrien-1-ol (i.e., no aggregation pheromone). Control group B: The only difference from the bait in Example 1 is that no tea polyphenols were added (i.e., no plant-derived feeding pheromones). Control group C: Commercially available termite bait (main ingredients are sugarcane bagasse, pine sawdust, sugar and 0.25% flufenoxuron).

[0058] Experimental Methods: Underground monitoring devices were evenly deployed in the same green area affected by Formosan subterranean termites. Ten replicates were set up for each of the treatment group, control group A, control group B, and control group C within the area. Data were recorded on days 7, 14, and 21 after placement. The results are shown in Table 7. ① The proportion of the device used for termite feeding on days 7, 14, and 21; ② Estimated order of magnitude of termites in each device (Level 1: 1-50 termites; Level 2: 51-200 termites; Level 3: >200 termites); ③ Weight of feed consumed on day 21 (g).

[0059] Table 7 Comparative Analysis of Synergistic Effects Table 7 shows that the experimental group was significantly better than all control groups in terms of feeding rate, aggregation degree, and consumption. Compared with control group A, the lack of cis-3,cis-6,trans-8-dodecanetrien-1-ol (aggregation pheromone) indicates that termites only eat and do not aggregate; compared with control group B, the lack of tea polyphenols (feeding pheromone) significantly reduces the initial discovery rate. Analysis of the results from the experimental and control groups demonstrates that cis-3,cis-6,trans-8-dodecanetrien-1-ol and tea polyphenols have a significant synergistic effect.

[0060] Example 5 Comprehensive application of bait in actual pest control scenarios: (1) Monitoring and gathering: In a city park where Formosan subterranean termites were suspected to be active, 20 standard bait boxes were randomly buried with a distance of more than 50 meters between adjacent bait boxes. Two pieces of bait prepared in Example 1 (about 20g / piece) were placed in each bait box. The boxes were checked every 7 days. The check found that on the 7th day, 12 boxes (60%) had termites feeding on the bait. On the 14th day, 18 boxes (90%) were invaded. Among them, 10 boxes (50%) had a large number of termites (≥ level 2) gathered. On average, the number of worker termites gathered in each box was more than 100, and soldier termites were also visible. (2) Centralized extermination: On the 14th day, use a powder sprayer to apply low-dose, slow-acting termite extermination powder (main component 0.5% fipronil powder) at a dosage of 6-10g / bucket to the bait blocks in all the bait boxes where termites have gathered. Then restore the boxes to their original state. Check again on the 7th, 14th and 30th days after the application. It was found that termite activity gradually decreased within 7 days after the application. After 14 days, there were no live termites in the boxes. After 30 days, the original infestation sites in the building were checked again and no new signs of live termite activity were found.

[0061] The results of Example 5 show that the bait provided by the present invention successfully and continuously attracts and gathers termites in the area to a controllable location. With the subsequent precise application of low-dose pesticides, the termite colony is effectively eliminated. The amount of pesticide used is reduced by more than 70% compared with the traditional general spraying method (the amount of pesticide used in the traditional general spraying method is about 20-30g), and it does not cause diffusion pollution to the environment. This fully demonstrates the advantages of the present invention in efficient aggregation and precise control.

[0062] In summary, this invention provides a safe, effective, and environmentally friendly termite control solution through ingenious component design. By deeply understanding the biological behavior of termites, it designs highly attractive and palatable bait, greatly enhancing existing, simple physical devices and achieving efficient, economical, and environmentally friendly termite monitoring and control. It has good industrialization prospects and socio-economic benefits.

[0063] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A plant-based, highly palatable bait for attracting termites, used in conjunction with a bait box or monitoring device, characterized in that, The ingredients include the following parts by weight: 1-50 parts of cis-3, cis-6, trans-8-dodecathorien-1-ol, 1-50 parts of tea polyphenols, 10-100 parts of microcrystalline cellulose, 1-50 parts of maltose, 1-100 parts of glucose, 1-50 parts of sucrose, 10-1000 parts of fermented sugarcane bagasse powder, 50-1000 parts of lignocellulose powder, 0-50 parts of pine wood extract, 1-50 parts of potassium sorbate, 1-100 parts of fungal extract, 50-1000 parts of acetone, 1-50 parts of termite nest fungal powder, and 50-1000 parts of binder.

2. The highly palatable plant-based bait for attracting termites to gather, used in conjunction with a bait box or monitoring device according to claim 1, is characterized in that... The lignocellulose powder is selected from at least one of Masson pine powder, eucalyptus powder, and sweetgum powder.

3. The highly palatable plant-based bait for attracting termites to gather, used in conjunction with a bait box or monitoring device according to claim 1, is characterized in that... The pine wood extract is selected from at least one of longifene, cedrol, and myrtol.

4. The highly palatable plant-based bait for attracting termites to gather, used in conjunction with a bait box or monitoring device according to claim 1, is characterized in that... The fungal extract is selected from at least one of Ganoderma lucidum extract, Ganoderma sinense extract, and Poria cocos extract.

5. The highly palatable plant-based bait for attracting termites to gather, used in conjunction with a bait box or monitoring device according to claim 1, is characterized in that... The termite nest fungal powder is a powder obtained by drying, sterilizing and pulverizing termite nests.

6. The highly palatable plant-based bait for attracting termites to gather, used in conjunction with a bait box or monitoring device according to claim 1, is characterized in that... The preparation method of the fermented sugarcane bagasse powder includes: crushing sugarcane bagasse to 100 mesh, adjusting the moisture content to 60-70%, then naturally fermenting it at 26-30℃ for 5-12 days, drying it to a moisture content ≤10%, crushing it again and passing it through an 80 mesh sieve to obtain the fermented sugarcane bagasse powder.

7. A method for preparing a highly palatable plant-based bait for attracting termites, as described in any one of claims 1 to 6, in conjunction with a bait box or monitoring device, characterized in that... Includes the following steps: (1) Mix microcrystalline cellulose, maltose, glucose, sucrose, fermented sugarcane bagasse powder, lignocellulose powder, pine wood extract, potassium sorbate, fungal extract and termite nest fungal nursery powder to obtain dry mixed base material; (2) Dissolve cis-3, cis-6, trans-8-dodecathien-1-ol and tea polyphenols in a portion of acetone to obtain a pheromone solution; add the pheromone solution to the dry-mixed base material to obtain a mixture. (3) Mix the adhesive with the remaining acetone and water to obtain an adhesive solution; mix the adhesive solution with the mixture obtained in step (2), and after molding and drying, obtain the plant-based highly palatable bait for attracting termites to gather, which is used in conjunction with the bait box or monitoring device.

8. The preparation method according to claim 7, characterized in that, In step (2), the pheromone solution is added by spraying.

9. The preparation method according to claim 7, characterized in that, In step (3), the drying process involves drying the bait at 40-50°C until the moisture content is 5-10%.

10. A method for termite control, characterized in that: The highly palatable plant-based bait for attracting termites, as described in any one of claims 1 to 6, is placed in the termite monitoring device or bait box to attract and gather termites.

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