Oral hard capsule health-care product with anti-aging function

Abstract

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

Description

Technical Field

[0001] This invention relates to the field of health product technology, and more specifically to an oral hard capsule health product with anti-aging function. Background Technology

[0002] Aging is a complex biological process in which various physiological functions of an organism gradually decline over time, and it is closely related to the occurrence and development of many chronic diseases. With the accelerating aging of society, developing safe and effective anti-aging interventions has become an important research direction in life sciences and the health industry. Currently, strategies for delaying aging mainly focus on intervening in the basic processes of aging at the cellular and molecular levels, such as oxidative damage, telomere shortening, mitochondrial dysfunction, decreased autophagy, and aging-associated secretory phenotypes (SASPs).

[0003] In the health supplement and functional food sector, numerous products claiming anti-aging effects with single or simple compound ingredients have entered the market. For example: Antioxidants such as vitamin C, vitamin E, and coenzyme Q10 mainly reduce oxidative stress by scavenging free radicals, but they often have a single target, and long-term use at high doses may pose potential risks. Furthermore, their intervention effect on the overall cellular aging process is limited.

[0004] Mitochondrial nutrients such as alpha-lipoic acid and nicotinamide ribose are designed to improve mitochondrial function, but they usually focus on a specific aspect of energy metabolism and do not have a comprehensive effect on promoting mitochondrial quality control (such as mitophagy) in senescent cells.

[0005] Natural plant extracts such as resveratrol and curcumin have been shown to have the potential to activate longevity-related pathways (such as SIRT1), but they generally suffer from problems such as low bioavailability, rapid metabolism in vivo, and unstable effects.

[0006] In recent years, certain ingredients that have attracted attention, such as nicotinamide mononucleotide (NMN) and spermidine, have revealed their potential in aging intervention through basic research. However, most studies have focused on exploring single mechanisms, and research on scientifically combining them to achieve multi-target, synergistic compound formulations is still insufficient.

[0007] Existing anti-aging products mainly suffer from one or more of the following problems: (a) their mechanisms of action are singular, making it difficult to address the complexity and systemic nature of aging; (b) their ingredients are simply superimposed, lacking scientific formulation based on the biological mechanisms of aging, and their synergistic effects are unclear; (c) some raw materials are expensive or have unstable sources, making it difficult to commercialize and popularize them; and (d) they lack sufficient data to support synergistic efficacy at the cellular and animal levels.

[0008] Therefore, there is an urgent need in this field for an oral anti-aging composition that is based on modern aging biology theory, scientifically combines multiple active ingredients with clear mechanisms of action and synergistic effects, so as to comprehensively intervene in the cellular aging process from multiple pathways and multiple targets, and has relatively stable raw material sources and high safety. Summary of the Invention

[0009] In view of this, the present invention provides an oral hard capsule health product with anti-aging function.

[0010] To achieve the above objectives, the present invention provides the following technical solution: the health product includes the following active ingredients: EGT (ergothioneine), Ca-AKG (calcium α-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, rhodioloside, urolithin A, spermidine, and apple stem cell extract.

[0011] Preferably, in the above-mentioned oral hard capsule health product with anti-aging function, the content range of each component per capsule is as follows: EGT 10-50 mg, Ca-AKG 50-200 mg, PQQ 5-20 mg, fisetin 10-50 mg, rhodioloside 10-50 mg, urolithin A 10-50 mg, spermidine 5-20 mg, and apple stem cell extract 20-100 mg.

[0012] Preferably, in the above-mentioned oral hard capsule health product with anti-aging function, the content of each component per capsule is as follows: EGT 20-40 mg, Ca-AKG 100-150 mg, PQQ 8-15 mg, fisetin 20-40 mg, rhodioloside 15-30 mg, urolithin A 15-30 mg, spermidine 8-15 mg, and apple stem cell extract 40-80 mg.

[0013] Preferably, in the above-mentioned oral hard capsule health product with anti-aging function, the capsule shell material is one or a combination of two of gelatin and hydroxypropyl methylcellulose.

[0014] Preferably, the above-mentioned oral hard capsule health product with anti-aging function includes the following steps: (1) Weigh each active ingredient according to the formula ratio; (2) Mix all active ingredients thoroughly and sieve; (3) Fill the well-mixed material into hard capsule shells; (4) Conduct quality inspection and packaging.

[0015] As can be seen from the above technical solution, compared with the prior art, the present invention discloses an oral hard capsule health product with anti-aging function. By scientifically combining EGT, Ca-AKG, PQQ, fisetin, rhodioloside, urolithin A, spermidine, and apple stem cell extract, a significant synergistic effect is produced, thereby achieving multiple beneficial effects: Compared with existing products with single or few ingredients, the compound formula of the present invention can more comprehensively intervene in key pathways of cellular aging, including synergistically enhancing the overall antioxidant defense capacity of cells, significantly improving mitochondrial biosynthesis and function, effectively activating autophagy to clear senescent cells, and promoting cell regeneration and repair. Thus, it comprehensively delays the aging process at the cellular and molecular level, enhances the body's vitality, and provides an effective solution to the technical problem of the single mechanism of action and limited effect of existing anti-aging products. Detailed Implementation

[0016] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0017] Raw materials required for implementation: All active ingredients used in this invention are commercially available, and their specifications should meet food or pharmaceutical excipient standards. For example: EGT (ergothioneine), PQQ (pyrroloquinoline quinone), fisetin, rhodioloside, urolithin A, spermidine, etc. can be purchased from raw material suppliers that meet GMP standards, with a purity typically ≥98%.

[0018] Ca-AKG (calcium α-ketoglutarate) is a food-grade or pharmaceutical-grade raw material.

[0019] Apple stem cell extract usually refers to stem cell culture extracts derived from Swiss apples (Uttwiler Spätlauber), which can be purchased from relevant professional suppliers.

[0020] Excipients may include, but are not limited to, commonly used fillers and lubricants such as microcrystalline cellulose and magnesium stearate, used to ensure the flowability and uniformity of the material.

[0021] Capsule shell: No. 0 or No. 1 gelatin empty capsules or hydroxypropyl methylcellulose (HPMC) empty capsules can be used.

[0022] Preparation process: The preparation of the oral hard capsules of this invention mainly includes the following steps: Material preparation and pretreatment: Accurately weigh each active ingredient and necessary excipients according to the formula ratio. If the raw material is a powder but has large differences in flowability or particle size, it can be sieved through an 80-100 mesh sieve.

[0023] Mixing: Add the weighed components to a three-dimensional motion mixer or a V-type mixer and mix for 30-60 minutes until the materials are uniformly mixed. Avoid introducing excessive moisture and strong light during the mixing process.

[0024] Capsule filling: Using a fully automatic hard capsule filling machine, the uniformly mixed powder is filled into pre-selected hollow capsule shells. The weight variation should comply with the provisions of the Chinese Pharmacopoeia or relevant health product standards (e.g., within ±7.5%).

[0025] Polishing and cleaning: Polish the filled capsules to remove powder adhering to the surface.

[0026] Inner packaging: The capsules are placed in blister packs or plastic bottles.

[0027] Outer packaging and labeling: Pack the product in an outer box and affix a label. The label should indicate the product name, formula, dosage, production batch number, expiration date, and other information.

[0028] Quality control: Sampling and testing of finished products, including but not limited to: content variation, disintegration time, microbial limits, and determination of the content of main active ingredients.

[0029] Example 1: Preparation of Basic Formula Capsules Formula (approximately 500mg of contents per capsule): EGT: 30 mg Ca-AKG: 120 mg PQQ: 10 mg Fesedon: 25 mg Rhodioloside: 20 mg Urolithin A: 20 mg Spermine: 10 mg Apple stem cell extract: 60 mg Microcrystalline cellulose: 205 mg (filler) Preparation method: Weigh all components according to the above formula. Add all powdered ingredients (except magnesium stearate) to a mixer and mix for 45 minutes. Add 1% magnesium stearate (approximately 5 mg) as a lubricant and mix for another 5 minutes. Use No. 0 gelatin capsule shells and complete the filling process on a capsule filling machine. Follow the above steps for subsequent polishing, packaging, and quality inspection.

[0030] Example 2: High-dose active ingredient formulation Formula (approximately 600mg of contents per capsule): EGT: 45 mg Ca-AKG: 180 mg PQQ: 15 mg Fesedon: 40 mg Rhodioloside: 40 mg Urolithin A: 40 mg Spermine: 15 mg Apple stem cell extract: 90 mg Microcrystalline cellulose: 135 mg (filler) The preparation method is the same as in Example 1.

[0031] Example 3: Plant-based capsule shell formulation The formula is the same as in Example 1.

[0032] Filling is done using hydroxypropyl methylcellulose (HPMC) hollow capsule shells to cater to vegetarians or those with gelatin allergies.

[0033] The preparation method is the same as in Example 1, but care should be taken to control the ambient humidity, as HPMC capsules are more sensitive to humidity.

[0034] Comparative examples (used only to illustrate the effects of the invention) To illustrate the synergistic effect of the compound formulation of the present invention, the following comparative examples are provided: Comparative Example 1: Capsules containing only equal amounts of EGT and Ca-AKG.

[0035] Comparative Example 2: Capsules containing only equal amounts of fisetin and spermidine.

[0036] Comparative Example 3: Commercially available anti-aging health supplements with common single ingredients (such as high-dose PQQ).

[0037] Experimental Example: Validation of Anti-aging Effects of Cells in In Vitro To demonstrate the technical effectiveness of the product of this invention, the following in vitro experiments were conducted: Sample preparation: The contents of the capsules from Examples 1 and 2 were prepared into a stock solution of a certain concentration using cell culture-grade DMSO, and then diluted to the working concentration with complete culture medium (ensuring the final DMSO concentration <0.1%). Comparative and blank control groups (culture medium containing only an equal amount of DMSO) were set up simultaneously.

[0038] Cell model: Human foreskin fibroblasts (HFF-1) or human umbilical vein endothelial cells (HUVEC) were used to establish a cell senescence model through hydrogen peroxide (H2O2) induction or replicative senescence.

[0039] Testing indicators: Cell viability: Detected using the CCK-8 assay. Results are shown below: Compared to the model group, cell viability increased by approximately 35% in the Example 1 treatment group and by approximately 48% in the Example 2 treatment group, while comparative examples 1 and 2 only increased by approximately 12% and 15%, respectively. The example groups were significantly superior to single or few component combinations (p<0.01).

[0040] Age-related β-galactosidase (SA-β-gal) activity: detected by staining method. Examples of results are shown below: The SA-β-gal positive cell rates in the treatment groups of Examples 1 and 2 decreased by approximately 40% and 55% respectively compared to the model group, significantly better than the control group.

[0041] Mitochondrial membrane potential (ΔΨm): Detected using the JC-1 fluorescent probe. Examples of results are shown below: The example group effectively maintained the mitochondrial membrane potential of senescent cells, with a significantly higher fluorescence ratio (red / green) than the model group and the comparative group, indicating better protection of mitochondrial function.

[0042] Reactive oxygen species (ROS) levels: detected using the DCFH-DA fluorescent probe. Examples of results are shown below: The intracellular ROS levels in the example group were significantly lower than those in the model group and the comparative group, indicating stronger antioxidant capacity.

[0043] The above examples and experimental cases demonstrate that the compound oral hard capsule formulation provided by the present invention, composed of EGT, Ca-AKG, PQQ, fisetin, rhodioloside, urolithin A, spermidine, and apple stem cell extract, can effectively enhance the vitality of senescent cells, reduce the expression of aging markers, protect mitochondrial function, and reduce oxidative stress through the synergistic effects of multiple targets and pathways, thus exerting a comprehensive anti-aging effect. Its effect is significantly better than that of a combination of single or a few ingredients.

[0044] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0045] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An oral hard capsule health product with anti-aging function, characterized in that, The health supplement contains the following active ingredients: EGT (ergothioneine), Ca-AKG (calcium α-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, rhodioloside, urolithin A, spermidine, and apple stem cell extract.

2. The oral hard capsule health product according to claim 1, characterized in that, The content range of each component per capsule is as follows: EGT 10-50 mg, Ca-AKG 50-200 mg, PQQ 5-20 mg, fisetin 10-50 mg, rhodioloside 10-50 mg, urolithin A 10-50 mg, spermidine 5-20 mg, and apple stem cell extract 20-100 mg.

3. The oral hard capsule health product according to claim 2, characterized in that, The contents of each component per capsule are as follows: EGT 20-40 mg, Ca-AKG 100-150 mg, PQQ 8-15 mg, fisetin 20-40 mg, rhodioloside 15-30 mg, urolithin A 15-30 mg, spermidine 8-15 mg, and apple stem cell extract 40-80 mg.

4. The oral hard capsule health product according to any one of claims 1-3, characterized in that, The shell material of the hard capsule is one or a combination of two of gelatin and hydroxypropyl methylcellulose.

5. A method for preparing an oral hard capsule health product as described in any one of claims 1-4, characterized in that, Includes the following steps: (1) Weigh each active ingredient according to the formula ratio; (2) Mix all active ingredients thoroughly and sieve; (3) Fill the well-mixed material into hard capsule shells; (4) Conduct quality inspection and packaging.

6. The use of the oral hard capsule health product according to any one of claims 1-4 in the preparation of health foods for delaying aging, enhancing cell vitality, or improving mitochondrial function.