Biological micropump gel for transdermal absorption and preparation method thereof

Abstract

According to the biological micropump gel for transdermal absorption and the preparation method of the biological micropump gel, based on the photosynthesis principle of algae, flowing of a micro-fluidic system is driven through the activity of algae cells, and the biological micropump gel is good in beautifying effect, easy and convenient to operate, high in safety, low in cost and suitable for large-scale popularization and application. And a better, more convenient and more practical medical beauty and skin care treatment method can be provided for the beauty and skin care field.

Description

Technical Field

[0001] This invention belongs to the field of beauty and skin care products, specifically a bio-micropump gel for transdermal absorption and its preparation method. Background Technology

[0002] In modern society, with environmental degradation, air pollution, and climate and geographical factors, coupled with a faster pace of life, increasing stress, irregular eating habits, and lack of attention to skincare, many people experience problems such as dry, dull skin, fine lines, and acne. Whether in the realm of oral beauty treatments, medical aesthetics, or cosmetics, anti-aging is undoubtedly a hot market.

[0003] Transdermal absorption technology and products are key to skin anti-aging. Currently, medical aesthetic clinics, hospital dermatology departments, and beauty salons generally use injections or phototherapy devices to "break the skin" for anti-aging treatments. There are currently few transdermal absorption methods and products besides injections or phototherapy devices that "break the skin." Injections or phototherapy devices can easily cause damage and side effects to the skin, require highly qualified and experienced operators, and are also relatively expensive. Therefore, transdermal absorption has always been a challenge in medical aesthetic dermatology. Summary of the Invention

[0004] To address the aforementioned problems in the prior art, this invention provides a bio-micropump gel for transdermal absorption and its preparation method. Based on the principle of photosynthesis in algae, it utilizes the activity of algal cells to drive the flow of a microfluidic system, thus solving the problem of transdermal absorption in medical aesthetics.

[0005] To achieve the above-mentioned objectives of the present invention, the technical solution of the present invention is as follows:

[0006] This invention discloses a bio-micropump gel for transdermal absorption. First, silica-containing microcrystals (which appear as glassy microneedles with a diameter of 10-100 nanometers under a microscope, hence the name "algal silica needles") are extracted from seaweed. These microcrystals can disrupt the protein structure of microorganisms or viruses and also have a relatively good impurity adsorption capacity. The gel is then prepared into a gel product suitable for application to the human body. The seaweed silica needles possess the unique cellular activity of algae, forming a seaweed silica needle-driven "microfluidic system" under the influence of photosynthesis or temperature, which we call a "bio-micropump" (also known as a "nanoscale bio-motor").

[0007] Specifically, a bio-micropump gel for transdermal absorption is made from the following raw materials in parts by weight:

[0008]

[0009] Preferably, the bio-micropump gel for transdermal absorption is made from the following raw materials in parts by weight:

[0010]

[0011] The aforementioned bio-micropump gel for transdermal absorption, wherein the seaweed silica needles are prepared from green algae, cyanobacteria, or red algae.

[0012] The preparation method of the seaweed silica needle in the aforementioned bio-micropump gel for transdermal absorption includes the following steps:

[0013] 1) Harvesting algae: Select algae strains suitable for silicon needle extraction, harvest the algae together with the culture medium, and ensure that a sufficient number of algal cells are collected;

[0014] 2) Removal of inorganic and organic matter: The harvested algae are processed by acid washing, alkali washing or high-temperature boiling to remove inorganic and organic matter attached to the cell surface;

[0015] 3) Cell disruption: The treated algal cells are disrupted by ultrasonic or high-pressure disruption to release the silica needles inside the cells.

[0016] 4) Silica needle separation: The broken algal cells are separated from the silica needles by centrifugation, followed by sieve separation or filter paper filtration.

[0017] 5) Cleaning and purification: The separated silicon needles are dissolved and precipitated by acetic acid or alcohol to clean and purify them, in order to remove impurities and improve purity;

[0018] 6) Drying and preparation: The cleaned and purified silicon needles are dried to obtain dry and pure silicon needles. Then, according to specific needs, the silicon needles are prepared into the required shape and particle size by grinding, dispersion and other methods.

[0019] The preparation method of the aforementioned bio-micropump gel for transdermal absorption includes the following steps:

[0020] 1) Prepare the necessary materials, including seaweed silica needles, hydrolyzed sodium hyaluronate, glycerin, conotoxin, 3-o-ethyl ascorbic acid, acetyl tetrapeptide-5, 1,2-pentanediol, p-hydroxyacetophenone, xanthan gum, allantoin, and soluble collagen.

[0021] 2) Weigh and add the required amount of material according to the desired gel concentration;

[0022] 3) Stir well, heat, and dissolve completely;

[0023] 4) Purify with alcohol and pour into a petri dish;

[0024] 5) Add BDDE crosslinking agent for crosslinking;

[0025] 6) Pour the cross-linked solvent into a filter for filtration;

[0026] 7) Remove cross-linking agents using a dialysis process;

[0027] 8) Particle classification is achieved through centrifugation;

[0028] 9) Gel filling;

[0029] 10) After UV light inspection, perform moist heat sterilization;

[0030] 11) After final inspection, package the finished biological micropump gel.

[0031] The aforementioned bio-micropump gel for transdermal absorption, wherein the preparation method of the bio-micropump includes the following steps:

[0032] 1) Preparation of seaweed extract for making bio-microneedles: Spirulina powder was mixed with sodium hydroxide solution and extracted at room temperature. The concentration of sodium hydroxide solution was 1.6%. The extraction time was 4 hours at room temperature. The mass ratio of spirulina powder to sodium hydroxide solution was 1:20. Spirulina residue was removed by high-speed centrifugation. The filtrate was collected and concentrated to a smaller volume by ultrafiltration to obtain a concentrated solution. The concentrated solution was subjected to alcohol precipitation at a volume ratio of 1:6 of anhydrous ethanol. After standing for 30 minutes, the precipitate was collected and decolorized by soaking in 95% ethanol. After decolorization, the precipitate was collected by filtration and then freeze-dried under vacuum.

[0033] 2) Preparation of crude bio-microneedles: The seaweed extract from step 1) was mixed with the culture medium and cultured at 25℃ for 20 days. After 20 days of culture, the cells of *Porphyra yezoensis* were removed by centrifugation at 5000 rpm for 10 minutes. The supernatant was collected and concentrated to a smaller volume to obtain a concentrate. Four times the volume of ethanol was added to the concentrate to obtain flocculent material. The flocculent material was collected and reconstituted with deionized water. It was then desalted using a dialysis bag and precipitated with four times the volume of ethanol. The flocculent material was collected and freeze-dried under vacuum to obtain crude bio-microneedles.

[0034] 3) Preparation of biological microneedle carrier: The crude biological microneedles obtained in step 2) were added to the culture medium and cultured at 30-35℃, while the bacterial culture temperature was 28℃, under light (4,000 Lx) for 5-7 days. The biological microneedle carrier was obtained by centrifugation at 5000g.

[0035] 4) Assembly of the biological micropump: Add 15 ml of liposome membrane buffer to the biological microneedle carrier obtained in step 3), suspend it, add 1 mg / ml of lysozyme, incubate on ice for 30 minutes, sonicate for 10 minutes, centrifuge at 25000g for 30 minutes, retain the supernatant, and then centrifuge the supernatant at 180000g at 4℃ for 90 minutes to precipitate a powdery complex, which is the biological micropump.

[0036] 5) Crystal extraction of the bio-micropump: The bio-micropump obtained in step 4) was mixed with sodium chloride solution and heated for extraction. The concentration of the sodium chloride solution was 1%, and the extraction conditions were 95°C for 4 hours. The mass ratio of bio-micropump to sodium chloride solution was 1:20. Impurities were removed by filtration through a silk sieve, and the filtrate was collected. The filtrate was concentrated to a smaller volume by ultrafiltration to obtain a concentrated solution. The solution was then subjected to alcohol precipitation at a volume ratio of 1:5 of the concentrated solution to 70% ethanol. After standing for 30 minutes, the precipitate was collected and washed three times with 75% ethanol. The bio-micropump crystal powder was obtained by vacuum freeze-drying.

[0037] The preparation of the liposome membrane in step 4) includes the following steps: using soybean lecithin, 100 mg of purified soybean lecithin is dissolved in chloroform, dried with nitrogen, and then an appropriate amount of ether is added and shaken, dried with nitrogen, sealed and stored at -20℃. Take 20 mg of the prepared lecithin in a small bottle, add 1 ml of Tris buffer, and sonicate in an ice bath for 3-5 minutes with a Cole Parmer CPX600 sonicator probe #13, with an amplitude between 5% and 10%, until the suspension is transparent, thus preparing the liposome membrane.

[0038] The preparation method of the bio-micropump gel for transdermal absorption includes the following steps:

[0039] 1) Prepare the necessary materials, including biological micropump crystals, sodium hyaluronate, glycerin, conopod peptides, oligopeptides, sodium chloride, and purified water;

[0040] 2) Weigh and add the required amount of material according to the desired gel concentration;

[0041] 3) Mix the biological micropump crystals, sodium chloride, and purified water evenly, heat until completely dissolved, purify with alcohol, and pour into a petri dish;

[0042] 4) Add sodium hyaluronate, glycerin, conopod peptides, oligopeptides and other ingredients to mix and achieve the required concentration and viscosity;

[0043] 5) Stir the mixed solution with a stirrer until the solution becomes a homogeneous gel, in order to form a stable gel structure;

[0044] 6) The prepared sodium hyaluronate gel needs to undergo quality testing to ensure it meets the expected standards. Further adjustments can be made if necessary.

[0045] When using this invention, first apply the bio-micropump gel of this invention to the face. After the algae are absorbed through the skin and the skin channels are opened, various facial masks, skin care products and other beauty products can then be used.

[0046] The beneficial effects of this invention are as follows:

[0047] The innovation of this invention lies in the use of a double-layer liposome process to encapsulate microcrystalline silicon needles into a "bio-micropump," and then preparing the "bio-micropump" into a gel suitable for application to the human body. This bio-micropump gel, used in the field of cosmetic skincare, exhibits a "transdermal absorption" effect, solving the problem of transdermal absorption in medical aesthetics. Based on the principle of algal photosynthesis, it utilizes the activity of algal cells to drive the flow of a microfluidic system, effectively addressing the challenge of transdermal absorption in medical aesthetics. It offers excellent transdermal absorption, is non-toxic and has no side effects on the skin, is easy to operate, and significantly reduces costs compared to injection or photoelectric devices. Attached Figure Description

[0048] Figure 1 This is a schematic diagram of the overall process flow of the present invention;

[0049] Figure 2 This is a flow chart of the seaweed silica needle preparation process of the present invention;

[0050] Figure 3 This is a schematic diagram of the Franz Cell diffusion cell in Embodiment 7 of the present invention;

[0051] Figure 4 This is a comparison diagram of nicotinamide penetration in Example 7 of the present invention;

[0052] Figure 5 This is a comparison chart of the statistical results of fluorescence intensity in the skin cell layer in Example 7 of the present invention;

[0053] Figure 6 This is a comparison chart of the diffusion percentage of nicotinamide in Example 7 of the present invention. Detailed Implementation

[0054] The technical solutions in the embodiments of this application are described clearly and completely below. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0055] Example 1

[0056] This embodiment provides a method for preparing seaweed extract for making bio-microcrystals (microneedles), including the following steps: mixing spirulina powder with sodium hydroxide solution and extracting at room temperature. The concentration of sodium hydroxide solution is 1.6%. The extraction is carried out at room temperature for 4 hours. The mass ratio of spirulina powder to sodium hydroxide solution is 1:20. Spirulina residue is removed by high-speed centrifugation. The filtrate is collected and concentrated to a smaller volume by ultrafiltration to obtain a concentrated solution. Alcohol precipitation is performed at a volume ratio of concentrated solution to anhydrous ethanol of 1:6. After standing for 30 minutes, the precipitate is collected and decolorized by soaking in 95% ethanol. After decolorization, the precipitate is collected by filtration and then freeze-dried under vacuum.

[0057] Example 2

[0058] This embodiment provides a method for preparing crude biocrystals (microneedles), including the following steps: seaweed extract is mixed with a culture medium and cultured at a temperature of 25°C for 20 days under a light intensity of 250 μmol photons / (m²s). After 20 days of culture, the cells of *Porphyra yezoensis* are removed by centrifugation at 5000 rpm for 10 minutes. The supernatant is collected and concentrated to a smaller volume to obtain a concentrate. Four times the volume of ethanol is added to the concentrate to obtain flocculent material. The flocculent material is collected, reconstituted with deionized water, desalted using a dialysis bag, and then precipitated with four times the volume of ethanol. The flocculent material is collected and freeze-dried under vacuum to obtain crude biocrystals (microneedles).

[0059] The culture medium was formulated as follows: natural seawater, NaNO3 (1.5 g / L), K2HPO4·3H2O (40 mg / L), NaHCO3 (2.0 g / L), CaCl2·2H2O (36 mg / L), FeCl3·6H2O (3.15 mg / L), citric acid (6.0 mg / L), EDTANa2·2H2O (4.36 mg / L), H3BO3 (2.86 mg / L), MnCl2·4H2O (1.18 mg / L), ZnSO4·7H2O (0.22 mg / L), Na2MoO4·2H2O (0.39 mg / L), Co(NO3)2·6H2O (0.05 mg / L) and CuSO4·5H2O (0.08 mg / L).

[0060] Example 3

[0061] This embodiment provides a method for preparing bio-microcrystals (microneedles), including the following steps:

[0062] Preparation of biocrystals (microneedles): Biocrystals (microneedles) obtained in Examples 1 and 2 were added to the culture medium. The bacteria were cultured at 28°C under light (4,000 Lx) for 5-7 days at a temperature of 30-35°C, and then centrifuged at 5000g to obtain the biocrystal (microneedle) carrier.

[0063] The culture medium formula is as follows: KH2PO4 1.0g, MgCl2 0.5g, CaCl2 0.1g, NaCl 1.0g, sodium acetate 1.0g, sodium succinate 1g, yeast extract 1.0g, NaHCO3 0.5g, peptone 0.5g, trace elements 1.0ml, vitamin solution 1.0ml, distilled water 1.0L, pH 6.8, 8g, autoclaved for 30 minutes. Vitamin solution ingredients: Biotin 0.1g, Niacin 0.35g, Thiamine dichloride 0.3g, Calcium pantothenate 0.1g, Vitamin B12 0.05g, Pyridoxolium hydrochloride (Fluka) 0.1g, Distilled water 1.0L; Trace element ingredients: FeC124H2O 1.8g, CoC126H2O 0.25g, NiC126H2O 0.01g, CuC1222H2O 0.01g, MnC124H2O 0.07g; ZnC12 0.1g, B2PO4 0.5g, Na2SeO25H2 0.01g, NaMoO4H2O 0.03g.

[0064] Example 4

[0065] This embodiment provides a method for assembling a biological micropump, including the following steps:

[0066] Bio-micropump assembly: 15 ml of bio-microcrystal (microneedle) carrier was added to liposome membrane buffer and suspended. 1 mg / ml of lysozyme was added, and the mixture was incubated on ice for 30 minutes. The mixture was then sonicated (20% amplitude, Cole Parmer CPX 600 sonicator, probe #13, Kunshan Ultrasonic Instrument Co., Ltd., KQ218 sonicator) for 10 minutes, centrifuged at 25,000g for 30 minutes, and the supernatant was retained. The supernatant was then centrifuged at 180,000g at 4℃ for 90 minutes, precipitating a powdery complex. This complex contains "active bio-microcrystals (microneedles)," and tiny bone needles of approximately 25–50 μm are visible under a microscope. This complex is figuratively called a "bio-micropump."

[0067] Preparation of liposome membranes: 100 mg of purified soybean phospholipids were dissolved in chloroform, dried with nitrogen, and then an appropriate amount of ether was added and shaken. The mixture was dried with nitrogen, sealed, and stored at -20°C. A small vial (20 mg) of the prepared phospholipids was taken, 1 ml of Tris buffer was added, and the mixture was sonicated in an ice bath for 3-5 minutes (amplitude between 5% and 10%) using a Cole Parmer CPX600 sonicator with probe #13 until the suspension became clear. This prepared liposome membrane.

[0068] Examples 1 / 2 / 3 / 4 complete the assembly of the seaweed-based bio-micropump of the present invention.

[0069] Example 5

[0070] This embodiment provides a microcrystal extraction method for preparing a bio-micropump for gel preparation, including the following steps:

[0071] The seaweed-derived bio-micropumps obtained in Examples 1 / 2 / 3 / 4 were mixed with a sodium chloride solution and heated for extraction. The concentration of the sodium chloride solution was 1%, and the extraction conditions were 95°C for 4 hours. The mass ratio of the pretreated bio-micropumps to the sodium chloride solution was 1:20. Impurities were removed by filtration through a silk sieve, and the filtrate was collected and concentrated to a smaller volume by ultrafiltration to obtain a concentrated solution. The concentrated solution was then subjected to alcohol precipitation at a volume ratio of 1:5 of 70% ethanol. After standing for 30 minutes, the precipitate was collected and washed three times with 75% ethanol. The precipitate was then freeze-dried under vacuum to obtain a crystalline powder containing bio-micropumps.

[0072] Example 6

[0073] This embodiment provides a method for preparing seaweed-based bio-micropump gels, including the following steps:

[0074] 1. Prepare the necessary materials: biological micropump crystals, sodium hyaluronate, glycerin, conopodeptide, oligopeptide, sodium chloride, and purified water.

[0075] 2. Weigh and add the required amount of material according to the desired gel concentration.

[0076] 3. Mix the biological micropump crystals, sodium chloride, and purified water thoroughly, then heat until completely dissolved. Purify with alcohol and pour into a petri dish.

[0077] 4. Add sodium hyaluronate, glycerin, conotoxin, oligopeptides and other ingredients to achieve the required concentration and viscosity.

[0078] 5. Stir the mixed solution with a stirrer until the solution becomes a homogeneous gel, in order to form a stable gel structure.

[0079] 6. The prepared sodium hyaluronate gel needs to undergo quality testing to ensure it meets the expected standards. Further adjustments can be made if necessary.

[0080] Example 7

[0081] This embodiment provides the preparation of seaweed-based bio-micropump gel for use in the field of beauty and skincare, and conducts qualitative and quantitative experiments on its transdermal absorption efficacy. The specific steps are as follows:

[0082] 1. Referring to OECD 428 and GB / T27818-2011, the corresponding operating procedures are described, and the Franz Cell diffusion pool is used for permeation.

[0083] Liquid is collected from the receiving pool at specific time points to detect the content of active ingredients and their metabolites. The permeation efficacy of the active ingredients is quantitatively assessed and reflected by the values ​​of cumulative permeation volume, permeability, and transdermal coefficient.

[0084] 2. Two pieces of piglet back skin, each 200±50 μm thick. Nicotinamide was applied directly to one piece, and after applying nicotinamide to the other piece, seaweed-based bio-micropump gel was evenly applied. The mixture was stirred using a magnetic stir bar, with physiological saline as the receiving solution, and the room temperature was maintained at 32℃±1℃ (close to human body surface temperature).

[0085] 3. Qualitative and quantitative indicators were used to detect the transdermal absorption of analytes containing fluorescein using the EpiKutis 3D epidermal model. The penetration of nicotinamide was observed at three time points: 2 hours, 6 hours, and 24 hours. Figure 3 As can be seen, niacinamide enters the skin cell layer and gradually penetrates into the basal layer over time, reaching the entire epidermis by 24 hours.

[0086] Figure 4 It is evident that the fluorescence signal of the skin cell layer is significantly enhanced after being encapsulated with the bio-micropump gel.

[0087] Nicotinamide itself has relatively good permeability, such as Figure 5 As shown, the penetration rate reached approximately 17% after 24 hours, and increased to 45% after application of the seaweed-based bio-micropump gel. This indicates that the bio-micropump gel has a significant and strong transdermal absorption effect.

[0088] Example 8

[0089] This embodiment provides a clinical treatment plan for immediate wrinkle removal utilizing the transdermal absorption effect of seaweed-based bio-micropump gel, and compares its advantages and disadvantages with traditional phototherapy, chemical fruit acid peels, and injectable mesotherapy. The specific steps are as follows:

[0090] A. Wrinkle-reducing effect after a single application

[0091] 1. Requirements for inclusion of participants: aged 25-50, male or female; 5-10 participants, with fine wrinkles on one of the following areas: nasolabial folds, corners of the eyes, and forehead (forehead wrinkles).

[0092] 2. Materials needed: Bio-micropump anti-wrinkle gel; conopod peptides, face mask, cream (purchased externally).

[0093] 3. Operating steps: Cleanse your face with saline solution → Apply "micro-pump gel + conotoxin" evenly to the face (focusing on the forehead, nasolabial folds, and crow's feet), massage for 3-5 minutes → apply a face mask for 10-15 minutes → apply essence cream.

[0094] 4. Effect evaluation: The transdermal absorption efficacy of the bio-micropump gel was qualitatively evaluated by photo comparison. The results showed that after using the bio-micropump anti-wrinkle gel of this invention, forehead wrinkles, nasolabial folds and crow's feet were significantly reduced.

[0095] B. Effects of three applications of water-light treatment

[0096] 1. Requirements for inclusion of participants: aged 25-50, male or female; 5-10 participants with dull, dry, and inelastic skin.

[0097] 2. Materials needed: Bio-micropump anti-wrinkle gel; hyaluronic acid, face mask, and cream (purchased externally).

[0098] 3. Operating steps: Cleanse your face with saline solution → Apply "micro-pump gel + hyaluronic acid" evenly to the face (focus on the T-zone), massage for 3-5 minutes → apply a face mask for 10-15 minutes → apply essence cream.

[0099] 4. After the first use, it is recommended to use it a second time after an interval of 3 to 5 days, and then use it a third time after an interval of 8 to 10 days; three times constitute a water light skin care treatment.

[0100] 5. Effect evaluation: The efficacy of "applying water light" was qualitatively evaluated by taking photos each time. The results showed that after using the bio-micro pump anti-wrinkle gel of this invention, pores can be refined, skin can be tightened, acne-prone skin can be repaired, and skin tone and wrinkles can be evened out.

[0101] Table 1 Comparison of Bio-micropump Therapy with Phototherapy and Chemical Acid Peeling Treatments

[0102]

[0103] Table 2 Comparison of Bio-micropump Aqua-Light and Medical Aesthetic Aqua-Light Treatments

[0104]

[0105]

[0106] The above examples illustrate that the bio-micropump gel of the present invention has good cosmetic effects, is simple and convenient to operate, has high safety, and low cost, and can provide better, more convenient and more practical medical aesthetic skin care treatment methods for the field of beauty and skin care.

[0107] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention are included within the protection scope of the present invention.

Claims

1. A bio-micropump gel for transdermal absorption, characterized in that... Made from the following parts by weight of raw materials:

2. The bio-micropump gel for transdermal absorption as described in claim 1, characterized in that... Made from the following parts by weight of raw materials:

3. The bio-micropump gel for transdermal absorption as described in claim 1, characterized in that... The preparation method of the seaweed silica needle includes the following steps: 1) Harvesting algae: Select algae strains suitable for silicon needle extraction, harvest the algae together with the culture medium, and ensure that a sufficient number of algal cells are collected; 2) Removal of inorganic and organic matter: The harvested algae are processed by acid washing, alkali washing or high-temperature boiling to remove inorganic and organic matter attached to the cell surface; 3) Cell disruption: The treated algal cells are disrupted by ultrasonic or high-pressure disruption to release the silica needles inside the cells. 4) Silica needle separation: The broken algal cells are separated from the silica needles by centrifugation, followed by sieve separation or filter paper filtration. 5) Cleaning and purification: The separated silicon needles are dissolved and precipitated by acetic acid or alcohol to clean and purify them, in order to remove impurities and improve purity; 6) Drying and preparation: The cleaned and purified silicon needles are dried to obtain dry and pure silicon needles. Then, according to specific needs, the silicon needles are prepared into the required shape and particle size by grinding, dispersion and other methods.

4. A bio-micropump gel for transdermal absorption as described in claim 1, characterized in that... Its preparation method includes the following steps: 1) Prepare the necessary materials, including seaweed silica needles, hydrolyzed sodium hyaluronate, glycerin, conotoxin, 3-o-ethyl ascorbic acid, acetyl tetrapeptide-5, 1,2-pentanediol, p-hydroxyacetophenone, xanthan gum, allantoin, and soluble collagen. 2) Weigh and add the required amount of material according to the desired gel concentration; 3) Stir well, heat, and dissolve completely; 4) Purify with alcohol and pour into a petri dish; 5) Add BDDE crosslinking agent for crosslinking; 6) Pour the cross-linked solvent into a filter for filtration; 7) Remove cross-linking agents using a dialysis process; 8) Particle classification is achieved through centrifugation; 9) Gel filling; 10) After UV light inspection, perform moist heat sterilization; 11) After final inspection, package the finished biological micropump gel.

5. A bio-micropump gel for transdermal absorption as described in claim 1, characterized in that... The preparation method of the biological micropump includes the following steps: 1) Preparation of seaweed extract for making bio-microneedles: Spirulina powder was mixed with sodium hydroxide solution and extracted at room temperature. The concentration of sodium hydroxide solution was 1.6%. The extraction time was 4 hours at room temperature. The mass ratio of spirulina powder to sodium hydroxide solution was 1:

20. Spirulina residue was removed by high-speed centrifugation. The filtrate was collected and concentrated to a smaller volume by ultrafiltration to obtain a concentrated solution. The concentrated solution was subjected to alcohol precipitation at a volume ratio of 1:6 of anhydrous ethanol. After standing for 30 minutes, the precipitate was collected and decolorized by soaking in 95% ethanol. After decolorization, the precipitate was collected by filtration and then freeze-dried under vacuum. 2) Preparation of crude bio-microneedles: The seaweed extract from step 1) was mixed with the culture medium and cultured at 25℃ for 20 days. After 20 days of culture, the cells of *Porphyra yezoensis* were removed by centrifugation at 5000 rpm for 10 minutes. The supernatant was collected and concentrated to a smaller volume to obtain a concentrate. Four times the volume of ethanol was added to the concentrate to obtain flocculent material. The flocculent material was collected and reconstituted with deionized water. It was then desalted using a dialysis bag and precipitated with four times the volume of ethanol. The flocculent material was collected and freeze-dried under vacuum to obtain crude bio-microneedles. 3) Preparation of biological microneedle carrier: The crude biological microneedles obtained in step 2) were added to the culture medium and cultured at 30-35℃, while the bacterial culture temperature was 28℃, under light (4,000 Lx) for 5-7 days. The biological microneedle carrier was obtained by centrifugation at 5000g. 4) Assembly of the biological micropump: Add 15 ml of liposome membrane buffer to the biological microneedle carrier obtained in step 3), suspend it, add 1 mg / ml of lysozyme, incubate on ice for 30 minutes, sonicate for 10 minutes, centrifuge at 25000g for 30 minutes, retain the supernatant, and then centrifuge the supernatant at 180000g at 4℃ for 90 minutes to precipitate a powdery complex, which is the biological micropump. 5) Crystal extraction of the bio-micropump: The bio-micropump obtained in step 4) was mixed with sodium chloride solution and heated for extraction. The concentration of the sodium chloride solution was 1%, and the extraction conditions were 95°C for 4 hours. The mass ratio of bio-micropump to sodium chloride solution was 1:

20. Impurities were removed by filtration through a silk sieve, and the filtrate was collected. The filtrate was concentrated to a smaller volume by ultrafiltration to obtain a concentrated solution. The solution was then subjected to alcohol precipitation at a volume ratio of 1:5 of the concentrated solution to 70% ethanol. After standing for 30 minutes, the precipitate was collected and washed three times with 75% ethanol. The bio-micropump crystal powder was obtained by vacuum freeze-drying.

6. A bio-micropump gel for transdermal absorption as described in claim 5, characterized in that... Step 4) involves the following steps in the preparation of the liposome membrane: 100 mg of purified soybean lecithin was dissolved in chloroform, dried with nitrogen, and then an appropriate amount of ether was added and shaken. The mixture was dried with nitrogen, sealed, and stored at -20°C. 20 mg of the prepared lecithin was taken from a small vial, 1 ml of Tris buffer was added, and the mixture was sonicated in an ice bath for 3-5 minutes with an amplitude between 5% and 10% using a Cole Parmer CPX600 sonicator with probe #13, until the suspension became transparent. This process was used to prepare the liposome membrane.

7. A bio-micropump gel for transdermal absorption as described in claim 5, characterized in that... The preparation method of the bio-micropump gel includes the following steps: 1) Prepare the necessary materials, including biological micropump crystals, sodium hyaluronate, glycerin, conopod peptides, oligopeptides, sodium chloride, and purified water; 2) Weigh and add the required amount of material according to the desired gel concentration; 3) Mix the biological micropump crystals, sodium chloride, and purified water evenly, heat until completely dissolved, purify with alcohol, and pour into a petri dish; 4) Add sodium hyaluronate, glycerin, conopod peptides, oligopeptides and other ingredients to mix and achieve the required concentration and viscosity; 5) Stir the mixed solution with a stirrer until the solution becomes a homogeneous gel, in order to form a stable gel structure; 6) The prepared sodium hyaluronate gel needs to undergo quality testing to ensure it meets the expected standards. Further adjustments can be made if necessary.

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