Anti-aging skin care product composition containing plant extracts and application of anti-aging skin care product composition

Abstract

The invention belongs to the field of skin care products, and discloses an anti-aging skin care product composition containing plant extracts and application of the anti-aging skin care product composition. The anti-aging composition containing the plant extract comprises a tricholoma matsutake extract, a silybum marianum seed extract, ergothioneine and a flaxseed extract, wherein the mass ratio of the tricholoma matsutake extract to the silybum marianum seed extract to the ergothioneine to the flaxseed extract is (4-8): (3-6): (0.02-0.05): (2-4). According to the anti-aging skin care product composition, various substances have a synergistic effect, non-enzymatic glycosylation reaction is inhibited to the maximum extent, the raw materials are wide in source and relatively low in price, and the anti-aging skin care product composition has an extremely good application prospect in the field of anti-aging skin care products.

Description

Technical Field

[0001] This invention relates to the field of skincare products, specifically to an anti-aging skincare composition containing plant extracts and its application. Background Technology

[0002] As the body's first line of defense, the skin plays a vital role in resisting various external aggressors, maintaining internal homeostasis, and ensuring the body's physiological functions. Skin aging is divided into two types: endogenous aging, which is the natural aging of the skin due to the decline in cell metabolism as we age; and exogenous aging, which is the aging of the skin caused by external environmental factors.

[0003] There are currently three main theories about aging: the free radical theory, the photoaging theory, and the glycation theory. The free radical theory posits that aging is the result of the accumulation of free radicals constantly produced by cells, and can be addressed through anti-oxidation and free radical scavenging. Currently, numerous antioxidants are used in beauty and skincare products and food. The photoaging theory argues that photoaging is unnatural aging caused by prolonged sun exposure, leading to melanin formation and damage to elastin structure, resulting in skin damage such as pigmentation, roughness, dryness, sagging, and wrinkles. Premature skin aging can be addressed through sun protection. The glycation theory suggests that proteins in our bodies aggregate with excess sugars. Initially, glycated proteins can revert to their original state, but prolonged contact with excess sugars leads to continuous deterioration, eventually forming advanced glycation end products (AGEs). With age, AGEs accumulate, causing skin to age, sag, and gradually become yellow and dull.

[0004] Compared to other theories, glycochemistry is a relatively recent development, only recently becoming a research hotspot. The pathways and influencing factors of glycation are still being explored. Currently, several non-enzymatic glycation inhibitors with anti-aging effects have been proposed. For example, Chinese patent CN116421482B discloses an anti-aging composition comprising matsutake mushroom extract, ergothioneine, and psoralen. This patent shows that the combination of matsutake mushroom extract, psoralen, and ergothioneine is significantly more effective than using any single component or simply combining two components, exhibiting a significant synergistic effect and significantly inhibiting non-enzymatic glycation, thus demonstrating excellent anti-aging properties. However, psoralen is expensive; adding it to skincare products would obviously increase their price, making them less economical and limiting their market reach. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the aforementioned background technology and provide an anti-aging skincare composition containing plant extracts and its application. The anti-aging skincare composition of this invention contains plant extracts such as matsutake mushroom extract and flaxseed extract. The synergistic effect of these multiple substances maximizes the inhibition of non-enzymatic glycosylation reactions. Furthermore, the raw materials are widely available and relatively inexpensive, making it a promising candidate for use in anti-aging skincare products.

[0006] To achieve the objectives of this invention, the anti-aging composition containing plant extracts of this invention includes matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract.

[0007] Matsutake mushrooms, scientifically known as *Tricholoma matsutake*, are also called pine mushrooms or matsutake fungi and are widely distributed in Northeast and Southwest my country. Matsutake mushrooms have a rich and diverse composition, mainly including polysaccharides, steroids, saponins, oils, triterpenes, and other active ingredients, as well as various minerals, vitamins, and amino acids. In recent years, numerous studies have used matsutake mushrooms or their extracts in cosmetic development, demonstrating that the active ingredients in matsutake extracts have significant effects on skin anti-aging, anti-oxidation, whitening, and anti-wrinkle effects. For example, Xu Lin et al. conducted scavenging tests on hydroxyl radicals and DPPH radicals, as well as non-enzymatic glycosylation reaction inhibition tests on matsutake extracts, showing that matsutake extracts have antioxidant effects, and finding that the scavenging or inhibition effects on hydroxyl radicals, DPPH radicals, and non-enzymatic glycosylation reactions are positively correlated with their content. Applying matsutake extracts to cosmetics aligns with the modern trend towards safe, natural, and green cosmetics.

[0008] Matsutake mushroom extract is a relatively mature commercial product and is relatively easy to purchase. The preparation method of matsutake mushroom extract in this invention refers to Chinese patent CN116421482B, namely: dried matsutake mushrooms are used, and 75-85% ethanol solution is added at a material-to-liquid ratio of 1:7-15. The mixture is then extracted in a water bath at 75-85℃ for 1-3 hours, and the extraction is repeated 2-3 times. The extract is then obtained by filtration, concentration, and dilution.

[0009] Preferably, in some embodiments of the present invention, the preparation method of the matsutake extract is as follows: dried matsutake mushrooms are used, and 75-80% ethanol solution is added at a material-to-liquid ratio of 1:8-12. The mixture is then extracted in a water bath at 80-85°C for 2-3 hours, and the extraction is repeated 2-3 times. The extract is then obtained by filtration, concentration, and dilution.

[0010] Ergothioneine (EGT), primarily derived from mushrooms, broccoli, and Brussels sprouts, is a compound discovered in 1909. It was initially found in the fungus *Claviceps purpurea*. The pure form is a white, water-soluble crystal. It is a natural antioxidant that protects cells in the human body and is an important active substance. As a natural antioxidant, ergothioneine is safe and non-toxic, possessing various physiological functions such as free radical scavenging, detoxification, maintaining DNA biosynthesis, normal cell growth, and cellular immunity. The ergothioneine used in this invention can be prepared using conventional methods in the art, or it can be purchased directly as a finished product with a purity of not less than 98%.

[0011] Milk thistle (Silybum marianum (L.) Gaertn.), also known as milk thistle, is an annual or biennial herb. It is bitter, cool in nature, and has heat-clearing, dampness-removing, liver-soothing, and gallbladder-benefiting properties. Milk thistle has been used medicinally since the fourth century BC. The 17th-century English herbalist Nicholas Culpepper described it as beneficial for treating jaundice and clearing liver and spleen obstruction. During the eclectic medicine movement (19th-20th centuries), milk thistle was used to treat varicose veins, dysmenorrhea, and congestion of the liver, spleen, and kidneys. In homeopathic medicine, tinctures of the seeds have been used to treat liver diseases, jaundice, gallstones, peritonitis, bleeding, bronchitis, and varicose veins. The main active ingredient in milk thistle is silymarin, which exerts its antioxidant effect by inducing superoxide dismutase, increasing cellular glutathione levels, and inhibiting lipid peroxidation; it is a direct free radical scavenger.

[0012] In some embodiments of the present invention, the method for preparing the milk thistle seed extract is as follows: crush dried milk thistle seeds, extract them by heating and refluxing with 65-80% ethanol solution at 100-110°C for 1-3 hours, extract 1-3 times, combine the obtained extracts, and then distill and concentrate under reduced pressure to obtain the extract.

[0013] Preferably, in some embodiments of the present invention, the method for preparing the milk thistle seed extract is as follows: crush dried milk thistle seeds, extract them by reflux with 65-75% ethanol solution at 100-105℃ for 1-2 hours, extract 1-3 times, combine the extracts, and then distill under reduced pressure and concentrate to obtain the extract.

[0014] Flaxseed can be processed into flaxseed oil, which we use daily. The benefits of flaxseed extract are often overlooked, but it actually plays a significant role and has considerable value. For example, flaxseed extract can improve intestinal function, increase absorption, enhance intestinal motility, and reduce constipation; it can also boost immunity, alleviate allergic reactions, and improve skin's lipid content, making the skin smoother, more moisturized, soft, and elastic, while normalizing skin respiration and perspiration, thus alleviating various skin problems…

[0015] In some embodiments of the present invention, the flaxseed extract is prepared by taking pulverized and dried flaxseeds and extracting them by supercritical fluid extraction.

[0016] Preferably, in some embodiments of the present invention, the conditions for supercritical fluid extraction are: pressure 30-35 MPa, separation temperature 50-60°C, extraction temperature 40-50°C, and extraction time 2-5 h.

[0017] Furthermore, in some embodiments of the present invention, the mass ratio of the matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract is 4-8:3-6:0.02-0.05:2-4. When the mass of the matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract is within the above-mentioned ratio range, the non-enzymatic glycosylation reaction can be inhibited to the maximum extent.

[0018] Furthermore, the present invention also provides the application of the aforementioned anti-aging composition containing plant extracts in the preparation of non-enzymatic glycosylation inhibitors.

[0019] In another aspect, the present invention also provides the application of the aforementioned anti-aging composition containing plant extracts in the preparation of anti-aging skin care products.

[0020] Preferably, the skincare product is a toner, lotion, cream, mask, or serum. When used in skincare products, in principle, the more of the composition used, the better the effect. However, the use of other excipients and the balance between effect and cost need to be considered. It is foreseeable that, without affecting the anti-aging effect of the plant extract-containing composition of this invention, the excipients that can be added to the skincare product include, but are not limited to, at least one of moisturizers, emollients, and preservatives.

[0021] Compared with the prior art, the advantages of the present invention are as follows:

[0022] (1) The anti-aging composition containing plant extracts of the present invention has low raw material cost, relatively simple preparation process, low procurement or preparation cost, and the raw materials can all be obtained from natural products. They are natural, green and safe raw materials with low toxicity and side effects, and are suitable for use in skin care products.

[0023] (2) The present invention combines four components, namely matsutake extract, milk thistle seed extract, ergothioneine and flaxseed extract, in a certain ratio. The effect is significantly better than the combination of individual components or two or three components, and the synergistic effect is significant. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. It should be understood that the following description is merely illustrative and not intended to limit the invention.

[0025] When a quantity, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pair of any upper or preferred value with any lower or preferred value, regardless of whether the range is disclosed individually. For example, when the range “1 to 5” is disclosed, the described range should be interpreted as including the ranges “1 to 4”, “1 to 3”, “1 to 2”, “1 to 2 and 4 to 5”, “1 to 3 and 5”, etc. When numerical ranges are described herein, unless otherwise stated, the range is intended to include its endpoints and all integers and fractions within that range.

[0026] The terms "one embodiment," "some implementations," "example," etc., used in this invention refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the technical features involved in the various embodiments of the invention can be combined with each other as long as they do not conflict with each other.

[0027] Example 1

[0028] An anti-aging composition containing plant extracts, comprising matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract, wherein the mass ratio of matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract is 6:5:0.03:3.

[0029] The preparation method of matsutake mushroom extract is as follows: dried matsutake mushrooms are used, and 80% ethanol solution is added at a material-to-liquid ratio of 1:10. The mixture is then heated in a water bath at 85°C for 3 hours. The extraction is repeated 3 times, and then filtered, concentrated, and diluted to obtain the extract.

[0030] The method for preparing the milk thistle seed extract is as follows: crush dried milk thistle seeds, extract them by reflux with 70% ethanol solution at 100℃ for 2 hours, extract three times, combine the extracts, and then distill and concentrate under reduced pressure to obtain the extract.

[0031] The flaxseed extract is prepared by taking pulverized and dried flaxseeds and extracting them by supercritical fluid extraction (pressure 33 MPa, separation temperature 55℃, extraction temperature 45℃) for 4 hours.

[0032] Example 2

[0033] An anti-aging composition containing plant extracts, comprising matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract, except that the mass ratio of matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract is 4:3:0.02:2, otherwise the same as in Example 1.

[0034] Example 3

[0035] An anti-aging composition containing plant extracts, comprising matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract, except that the mass ratio of matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract is 8:6:0.05:4, otherwise the same as in Example 1.

[0036] Comparative Example 1

[0037] An anti-aging composition containing plant extracts, comprising matsutake mushroom extract and milk thistle seed extract, is identical to Example 1 except that the mass ratio of matsutake mushroom extract to milk thistle seed extract is 6:5.

[0038] Comparative Examples 2-10

[0039] Comparative Examples 2-10 are anti-aging compositions containing plant extracts, except that the components and their proportions are as shown in Table 1, and are otherwise identical to those in Example 1.

[0040] Table 1. Components and proportions of each embodiment and comparative example.

[0041] Example Components Proportion Example 1 Matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract 6：5：0.03：3 Example 2 Matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract 4：3：0.02：2 Example 3 Matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract 8：6：0.05：4 Comparative Example 1 Matsutake mushroom extract, milk thistle seed extract 6：5 Comparative Example 2 Matsutake mushroom extract, flaxseed extract 6：3 Comparative Example 3 Milk thistle seed extract, ergothioneine 5：0.03 Comparative Example 4 Milk thistle seed extract, flaxseed extract 5：3 Comparative Example 5 Matsutake mushroom extract, milk thistle seed extract, ergothioneine 6：5：0.03 Comparative Example 6 Matsutake mushroom extract, milk thistle seed extract, flaxseed extract 6：5：3 Comparative Example 7 Matsutake mushroom extract, ergothioneine, flaxseed extract 6：0.03：3 Comparative Example 8 Milk thistle seed extract, ergothioneine, flaxseed extract 5：0.03：3 Comparative Example 9 Matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract 10：2：0.03：3 Comparative Example 10 Matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract 3：8：0.03：3

[0042] Example of effect

[0043] The inhibitory effect of the compositions of each embodiment and comparative example on non-enzymatic glycosylation reaction was tested with reference to Chinese Patent CN116421482B.

[0044] Add 19.82g glucose, 0.4024g bovine serum albumin and 0.39g sodium azide to 100mL of 0.2mol / L phosphate buffer (pH=7.4), mix well, and the reaction solution is formed.

[0045] The compositions of each example and comparative example were prepared into test solutions of different concentrations using distilled water, with concentrations of 50 μg / mL, 200 μg / mL, and 800 μg / mL.

[0046] Eleven test tubes were taken, and 4 mL of reaction solution and 1 mL of test solution of different concentrations were added to each tube sequentially, so that the total volume of solution in all test tubes was 5 mL. The initial fluorescence value of all samples was measured using a fluorescence spectrophotometer. Then, all test tubes were placed in a 37℃ water bath in the dark for 7 days, and the fluorescence value of the samples in each test tube was measured again. This process was repeated for another 7 days, after which the fluorescence value was measured again. The test conditions were: excitation wavelength 370 nm, slit width 5 nm; emission wavelength range 380–500 nm, slit width 5 nm; light source voltage 700 V.

[0047] The inhibition results of the non-enzymatic glycosylation reaction of the compositions of each example and comparative example are shown in Table 2 below.

[0048] Table 2. Inhibition rate of non-enzymatic glycosylation reaction by the compositions of each example and comparative example.

[0049]

[0050] As shown in Table 2, the compositions of all examples and comparative examples exhibited activity in inhibiting non-enzymatic glycosylation reactions, but the inhibitory effects on non-enzymatic glycosylation reactions differed significantly. Among them, the compositions of Examples 1-3, containing matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract, with a mass ratio of 4-8:3-6:0.02-0.05:2-4, showed the best inhibition rate of non-enzymatic glycosylation reactions, reaching up to 83%. Although the compositions of Comparative Examples 9-10 also contained matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract, the mass ratio of these ingredients was not optimal, resulting in a poorer inhibition rate of non-enzymatic glycosylation reactions. The compositions of Comparative Examples 1-8 contained only two or three of the following: matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract. Their inhibitory effect on non-enzymatic glycosylation reactions was significantly less than that of Examples 1-3 and Comparative Examples 9-10.

[0051] The above results indicate that the present invention, by combining the four components of matsutake mushroom extract, milk thistle seed extract, ergothioneine and flaxseed extract in a ratio of 4-8:3-6:0.02-0.05:2-4, has a significantly better effect than using two or three of the components, and exhibits a significant synergistic effect.

[0052] Those skilled in the art will readily understand that the above description is merely an embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. An anti-aging composition containing plant extracts, characterized in that, The anti-aging composition containing plant extracts includes matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract.

2. The anti-aging composition containing plant extracts according to claim 1, characterized in that, The method for preparing the matsutake mushroom extract is as follows: dried matsutake mushrooms are used, and 75-85% ethanol solution is added at a material-to-liquid ratio of 1:7-15. The mixture is then extracted in a water bath at 75-85℃ for 1-3 hours, and the extraction is repeated 2-3 times. The extract is then obtained by filtration, concentration, and dilution.

3. The anti-aging composition containing plant extracts according to claim 1, characterized in that, The method for preparing the matsutake mushroom extract is as follows: dried matsutake mushrooms are used, and 75-80% ethanol solution is added at a material-to-liquid ratio of 1:8-12. The mixture is then extracted in a water bath at 80-85℃ for 2-3 hours, and the extraction is repeated 2-3 times. The extract is then obtained by filtration, concentration, and dilution.

4. The anti-aging composition containing plant extracts according to claim 1, characterized in that, The method for preparing the milk thistle seed extract is as follows: crush dried milk thistle seeds, heat and reflux with 65-80% ethanol solution at 100-110℃ for 1-3 hours, extract 1-3 times, combine the obtained extracts and then distill under reduced pressure and concentrate to obtain the extract.

5. The anti-aging composition containing plant extracts according to claim 1, characterized in that, The method for preparing the milk thistle seed extract is as follows: crush dried milk thistle seeds, reflux extract with 65-75% ethanol solution at 100-105℃ for 1-2 hours, extract 1-3 times, combine the extracts, and then distill under reduced pressure and concentrate to obtain the extract.

6. The anti-aging composition containing plant extracts according to claim 1, characterized in that, The flaxseed extract is prepared by supercritical fluid extraction of pulverized and dried flaxseed.

7. The anti-aging composition containing plant extracts according to claim 6, characterized in that, The conditions for supercritical fluid extraction are: pressure 30-35 MPa, separation temperature 50-60℃, extraction temperature 40-50℃, and extraction time 2-5 h.

8. The anti-aging composition containing plant extracts according to any one of claims 1-7, characterized in that, The mass ratio of the matsutake mushroom extract, milk thistle seed extract, ergothioneine, and flaxseed extract is 4-8:3-6:0.02-0.05:2-4.

9. The use of the anti-aging skincare composition containing plant extracts according to any one of claims 1-8 in the preparation of a non-enzymatic glycosylation inhibitor.

10. The use of the anti-aging skincare composition containing plant extracts according to any one of claims 1-8 in the preparation of anti-aging skincare products; preferably, the skincare product is a skincare water, lotion, cream, mask or serum.

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