Method for efficiently degrading kitchen garbage by using microbial nano manganese oxide composite material and application of microbial nano manganese oxide composite material

Abstract

The invention relates to a method for efficiently degrading kitchen garbage by using a microbial nano manganese oxide composite material and application of the microbial nano manganese oxide composite material. According to the method, firstly, the kitchen garbage is used as an inoculation source, indigenous microorganisms with high degradation capacity in the natural degradation process of the kitchen garbage are successfully enriched, and the enriched microbial flora takes leuconostoc and saccharomyces cerevisiae as dominant bacteria; secondly, nano manganese oxide is used as a catalyst, microorganisms and nano particles have a synergistic effect, and a microorganism nano manganese oxide composite material is obtained; the composite material can be applied to kitchen garbage treatment, 85%-99% of the weight of kitchen garbage can be reduced within 2-4 h under the aerobic condition at the temperature of 15-30 DEG C, and metabolic liquid products with high biodegradability are generated. According to the invention, the nano material and the microorganisms are creatively combined, and the resourceful treatment of the kitchen garbage is accelerated by utilizing the interaction between the nano material and the microorganisms.

Description

Technical Field

[0001] This invention relates to the field of kitchen waste resource utilization technology, specifically to a method and application of a microbial nano-manganese oxide composite material for the efficient degradation of kitchen waste. Background Technology

[0002] Food waste mainly consists of food scraps, grease, paper towels, fruit peels, vegetable leaves, and other organic matter. It has a high water content and is rich in organic matter such as protein, carbohydrates, and fats. With the acceleration of urbanization, the amount of food waste generated is increasing year by year, becoming one of the largest components of urban waste. However, traditional food waste treatment methods have many problems, such as landfilling occupying land resources, and incineration causing air pollution and failing to fully recover the organic components. Traditional food waste treatment methods generally rely on physical, chemical, or high-temperature treatment technologies, but these methods usually cannot completely degrade organic matter and are costly and energy-intensive. Therefore, finding an environmentally friendly and efficient food waste treatment method has become an urgent challenge.

[0003] Microbial degradation is a widespread organic matter degradation process in nature. Specific microorganisms (such as bacteria, fungi, and actinomycetes) can decompose organic matter through their enzymatic systems, converting it into harmless simple substances such as carbon dioxide, water, and mineral salts. The advantages of microbial degradation of organic waste are: minimal environmental impact, low energy consumption, and low resource consumption during the degradation process. However, despite the great potential of microbial degradation methods, some problems exist in practical applications: on the one hand, the degradation rate of some organic pollutants is slow, making it difficult to effectively treat high concentrations of organic waste; on the other hand, under complex environmental conditions, the activity of microorganisms may be inhibited, leading to unstable degradation results. Therefore, the introduction of a microbial nano-manganese oxide composite material is particularly important—it can not only participate in the degradation of organic pollutants as a catalyst but also activate the oxidation reaction of certain organic molecules, and it has already been widely used in environmental protection and wastewater treatment. The nano-manganese oxide in the composite material decomposes complex organic matter through catalytic oxidation reactions, generating simpler compounds, thereby reducing the difficulty of microbial degradation. Microorganisms then further degrade these products into harmless substances (such as carbon dioxide and water) through metabolic activities. This synergistic effect can significantly improve the efficiency of waste degradation, especially for complex organic matter such as oils, starches, and proteins. Summary of the Invention

[0004] To address the aforementioned problems in the pretreatment of kitchen waste, this invention provides a method for the efficient degradation of kitchen waste using a microbial nano-manganese oxide composite material. By combining the organic degradation capabilities of microorganisms with the catalytic properties of nano-manganese oxide, the organic matter in kitchen waste can be efficiently degraded, maximizing the degradation efficiency of the microorganisms and providing an innovative solution for kitchen waste treatment.

[0005] The technical solution adopted by the present invention to achieve the above objectives is as follows: This invention provides a method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: Step (1) Enrichment and cultivation of microbial communities for degrading kitchen waste: Using kitchen waste / food waste as the inoculum source, continuous subculture and domestication are carried out using culture medium, followed by shaking and centrifugation to collect microbial cells; wherein, Leuconostoc mesenteroides and Saccharomyces cerevisiae are the dominant microbial genera in the microbial cells. Step (2) Preparation of microbial nano-manganese oxide composite material: Using manganese chloride as the manganese source, the manganese salt is dissolved in sterile water to obtain a manganese salt mixture solution; the manganese salt mixture solution is added to sodium hydroxide solution, magnetically stirred, and tannic acid is added as a complexing agent, and the reaction is carried out for 1~3 h; then centrifugation, rinsing and drying are performed to obtain nano-manganese oxide with catalytic properties; the nano-manganese oxide particles are dissolved in MOPs buffer solution to obtain nano-manganese oxide solution; the microbial cells are mixed with the nano-manganese oxide solution, the concentration of the microbial cells is controlled at 0.1 g / L~6 g / L, and incubated at 20℃ and 100 rpm for 2 h, and centrifuged to obtain microbial nano-manganese oxide composite material.

[0006] Preferably, in step (1), the inoculation source is kitchen waste / food waste placed for 1 to 2 days, the inoculation amount is 30 g / L to 300 g / L, the culture conditions are 20℃ to 40℃, and the shaking parameters are 120 rpm to 200 rpm.

[0007] Preferably, in the microbial cells, Leuconostoc mesenteroides has a relative content of 35% to 45% at the bacterial level, and Saccharomyces cerevisiae has a relative content of 85% to 95% at the fungal level.

[0008] Preferably, in step (2), the concentration of manganese chloride is 2 g / L to 5 g / L; the mass fraction of the sodium hydroxide solution is 5% to 10%; the volume ratio of the manganese salt mixture solution to the sodium hydroxide solution is 1:3; the mass fraction of the tannic acid is 0.1% to 1%; and the nano manganese oxide solution is prepared by dissolving 1 g to 10 g of the nano manganese oxide in 2 LMOPs buffer solution.

[0009] Preferably, in step (2), the ratio of nano-manganese oxide to microbial cells is 0.6 to 3.5.

[0010] Preferably, in step (2), the incubation parameters are: the concentration of the microbial cells is 0.1 g / L to 6 g / L, and the cells are incubated at 20°C and 100 rpm for 2 h.

[0011] The present invention also provides a microbial nano-manganese oxide composite material prepared by the above-mentioned method for preparing microbial nano-manganese oxide composite material.

[0012] This invention also provides the application of the microbial nano-manganese oxide composite material described above in the field of degrading kitchen waste / food waste.

[0013] Preferably, the degradation method of the kitchen waste / food waste includes: thoroughly mixing the microbial nano-manganese oxide composite material with the kitchen waste / food waste, stirring at 30 r / min to 50 r / min, controlling the reaction temperature at 15℃ to 30℃, mechanically stirring under aerobic conditions, and completing the degradation after 2 h to 4 h of reaction.

[0014] Furthermore, the specific addition amounts are as follows: the microbial nano-manganese oxide composite material is 1 g / L~12 g / L, and the kitchen waste / food waste is 300 g / L~1200 g / L; the ratio of the microbial nano-manganese oxide composite material to kitchen waste / food waste is 1:(100~900); after the degradation is completed, the weight reduction rate of kitchen waste / food waste is 85%~99%, and the metabolic liquid products BOD5 / COD Cr The value ranges from 0.55 to 0.88.

[0015] This invention provides a method for the efficient degradation of kitchen waste using microbial nano-manganese oxide composite materials, which has the following significant advantages compared to existing technologies: 1. Nano-manganese oxide possesses oxidizing and catalytic properties. It can release free electrons, increasing the number of active groups in the reaction system, and subsequently forming humic substances through the condensation of phenols, reducing sugars, and amino acids. Furthermore, the presence of nano-manganese oxide accelerates the degradation of organic components and increases humic substance concentration by driving changes in functional group concentration, thus accelerating the transformation of kitchen waste into high-quality products. On one hand, nano-manganese oxide can act as an enzyme promoter to stimulate enzyme activity, stimulating microorganisms to decompose and utilize organic matter; on the other hand, introducing nano-manganese oxide into the system can alter microbial diversity, reconstruct the microbial community degradation system, and significantly accelerate the microbial metabolic rate, thereby effectively solving the problems of volume reduction, harmlessness, and resource recovery in the kitchen waste treatment industry.

[0016] 2. The microbial flora used is screened from kitchen waste, making it highly targeted. The dominant bacteria, *Leuconostoc mesenteroides* and *Saccharomyces cerevisiae*, can efficiently degrade the main components of kitchen waste, such as starch, protein, and cellulose, rapidly converting complex insoluble organic matter into simple, easily degradable organic matter. Especially under the influence of nano-manganese oxide, it possesses nanoenzyme activity, further enabling the decomposition of hydrolysis products, forming high BOD5 / COD ratios. Cr (0.55~0.88) of liquid metabolites, thereby improving the resource utilization of hydrolysis products.

[0017] 3. The preparation method of microbial nanomaterial composites is simple, efficient, and low-cost. The microbial community can be cultured using classic LB medium, which has simple culture conditions and is easy to scale up. Tannic acid is added as a complexing agent in the preparation of nano-manganese oxide. The unique phenolic hydroxyl structure and electron-donating properties of tannic acid can induce the formation of sub-8 nm manganese oxide particles, which significantly improves the activity compared with conventional nano-metal oxides.

[0018] 4. During the degradation of kitchen waste, the nanocomposite material does not require continuous addition and can degrade efficiently at room temperature without the need for additional heat. The reaction cycle is short; under anaerobic or aerobic conditions, 85%–99% of organic kitchen waste can be converted into small-molecule organic acid liquid metabolites within 2–4 hours, achieving a waste reduction rate of up to 99%. Furthermore, the annual loss rate of the nanocomposite material is less than 5%, demonstrating advantages such as low energy consumption, high efficiency, and no secondary pollution. Detailed Implementation

[0019] The present invention will now be described in detail with reference to specific embodiments.

[0020] Example 1: A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: Weigh 30 g of kitchen waste that had been stored for 1 day and add it to 1 L LB medium. Shake overnight at 20°C and 120 rpm. The obtained microbial community was dominated by Leuconostoc mesenteroides and Saccharomyces cerevisiae. After enrichment culture, the relative content of Leuconostoc mesenteroides at the bacterial level was 35%, and the relative content of Saccharomyces cerevisiae at the fungal level was 85%. The microbial cells were collected by centrifugation.

[0021] 2.205 g of manganese chloride was dissolved in 1000 mL of sterile water, denoted as solution A. Then, 200 mL of solution A was taken and 600 mL of 5% sodium hydroxide solution (solution B) was added, maintaining a volume ratio of B:A = 3. The mixture was stirred thoroughly with a magnetic stirrer, with 0.08 g of tannic acid added as a complexing agent, and allowed to react for 1 hour. After centrifugation, rinsing, and drying, nano-manganese oxide with catalytic properties was obtained. 1.0 g of nano-manganese oxide was dissolved in 2 LMOPs buffer solution, and then 0.2 g of microbial cells were added and mixed with the nano-manganese oxide solution, maintaining a microbial cell concentration of 0.1–6 g / L. The mixture was incubated at 20℃ and 100 rpm for 1 hour. The nano-manganese oxide adhered to the bacterial surface or bacterial appendages, spontaneously forming a microbial nano-manganese oxide composite ecological community. Centrifugation was then used to obtain the microbial nano-manganese oxide composite material.

[0022] In a 1 L container, 1.0 g of microbial nano-manganese oxide composite material and 300 g of kitchen waste were added and thoroughly mixed. No acclimatization time was required; the mixture was stirred directly at 30 r / min, with the reaction temperature controlled at 15℃. After 2 hours of reaction, the kitchen waste weight reduction rate was 85%, and solid organic matter was converted into liquid organic acid metabolites. The metabolic liquid products BOD5 / COD ratio was [not specified]. Cr It is 0.55.

[0023] Example 2: A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: 300g of kitchen waste that had been stored for 2 days was weighed and added to 1 L LB medium, and incubated overnight at 40℃ and 200 rpm with shaking. The obtained microbial community was dominated by Leuconostoc mesenteroides and Saccharomyces cerevisiae. After enrichment culture, the relative content of Leuconostoc mesenteroides at the bacterial level was 45%, and the relative content of Saccharomyces cerevisiae at the fungal level was 95%. The microbial cells were collected by centrifugation.

[0024] 4.995 g of manganese chloride was dissolved in 1000 mL of sterile water, denoted as solution A. Then, 100 mL of solution A was taken and 300 mL of 10% sodium hydroxide solution (solution B) was added, maintaining a volume ratio of B:A = 3. The mixture was stirred thoroughly with a magnetic stirrer, with 0.8 g of tannic acid added as a complexing agent, and allowed to react for 1 hour. After centrifugation, rinsing, and drying, nano-manganese oxide with catalytic properties was obtained. 10.0 g of nano-manganese oxide was dissolved in 2 LMOPs buffer solution, and then 12.0 g of microbial cells were added and mixed with the nano-manganese oxide solution, maintaining a microbial cell concentration of 0.1–6 g / L. The mixture was incubated at 20℃ and 100 rpm for 1 hour. The nano-manganese oxide adhered to the bacterial surface or bacterial appendages, spontaneously forming a microbial nano-manganese oxide composite ecological community. Centrifugation was then used to obtain the microbial nano-manganese oxide composite material.

[0025] In a 1 L container, 12.0 g of microbial nano-manganese oxide composite material and 1200 g of kitchen waste were added and thoroughly mixed. No acclimatization time was required; the mixture was stirred directly at 50 r / min, with the reaction temperature controlled at 15℃. After 4 hours of reaction, the kitchen waste weight reduction rate was 99%, and solid organic matter was converted into liquid organic acid metabolites. The metabolic liquid products BOD5 / COD ratio was [not specified]. Cr It is 0.88.

[0026] Example 3: A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: 200g of kitchen waste that had been stored for 2 days was weighed and added to 1 L LB medium, and incubated overnight at 30°C and 180 rpm with shaking. The obtained microbial community was dominated by Leuconostoc mesenteroides and Saccharomyces cerevisiae. After enrichment culture, the relative content of Leuconostoc mesenteroides at the bacterial level was 40%, and the relative content of Saccharomyces cerevisiae at the fungal level was 90%. The microbial cells were collected by centrifugation.

[0027] 2.995 g of manganese chloride was dissolved in 1000 mL of sterile water, denoted as solution A. Then, 100 mL of solution A was taken and 300 mL of 10% sodium hydroxide solution (solution B) was added, maintaining a volume ratio of B:A = 3. The mixture was stirred thoroughly with a magnetic stirrer, with 0.3 g of tannic acid added as a complexing agent, and allowed to react for 1 hour. After centrifugation, rinsing, and drying, nano-manganese oxide with catalytic properties was obtained. 3.0 g of nano-manganese oxide was dissolved in 2 LMOPs buffer solution, and then 0.4 g of microbial cells were added and mixed with the nano-manganese oxide solution, maintaining a microbial cell concentration of 0.1–6 g / L. The mixture was incubated at 20℃ and 100 rpm for 1 hour. The nano-manganese oxide adhered to the bacterial surface or bacterial appendages, spontaneously forming a microbial nano-manganese oxide composite material ecological community. Centrifugation was then used to obtain the microbial nano-manganese oxide composite material.

[0028] In a 1 L container, 10.0 g of microbial nano-manganese oxide composite material and 1200 g of kitchen waste were added and thoroughly mixed. No acclimatization time was required; the mixture was stirred directly at 45 r / min, with the reaction temperature controlled at 15℃. After 3 hours of reaction, the kitchen waste weight reduction rate was 90%, and solid organic matter was converted into liquid organic acid metabolites. The metabolic liquid products BOD5 / COD ratio was [not specified]. Cr It is 0.78.

[0029] Example 4: A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: 150g of kitchen waste that had been stored for 2 days was weighed and added to 1 L LB medium. The mixture was shaken overnight at 30°C and 150 rpm. The obtained microbial community was dominated by Leuconostoc mesenteroides and Saccharomyces cerevisiae. After enrichment culture, the relative content of Leuconostoc mesenteroides at the bacterial level was 42%, and the relative content of Saccharomyces cerevisiae at the fungal level was 92%. The microbial cells were collected by centrifugation.

[0030] 2.395 g of manganese chloride was dissolved in 1000 mL of sterile water, denoted as solution A. Then, 100 mL of solution A was taken and 300 mL of 8% sodium hydroxide solution (solution B) was added, maintaining a volume ratio of B:A = 3. The mixture was stirred thoroughly with a magnetic stirrer, with 0.5 g of tannic acid added as a complexing agent, and allowed to react for 1 hour. After centrifugation, rinsing, and drying, nano-manganese oxide with catalytic properties was obtained. 3.0 g of nano-manganese oxide was dissolved in 2 LMOPs buffer solution, and then 0.6 g of microbial cells were added and mixed with the nano-manganese oxide solution, maintaining a microbial cell concentration of 0.1–6 g / L. The mixture was incubated at 20℃ and 100 rpm for 1 hour. The nano-manganese oxide adhered to the bacterial surface or bacterial appendages, spontaneously forming a microbial nano-manganese oxide composite material ecological community. Centrifugation was then used to obtain the microbial nano-manganese oxide composite material.

[0031] In a 1 L container, 10.0 g of microbial nano-manganese oxide composite material and 1200 g of kitchen waste were added and thoroughly mixed. No acclimatization time was required; the mixture was stirred directly at 50 r / min, with the reaction temperature controlled at 15℃. After 4 hours of reaction, the kitchen waste weight reduction rate was 90%, and solid organic matter was converted into liquid organic acid metabolites. The metabolic liquid products BOD5 / COD ratio was [not specified]. Cr It is 0.80.

[0032] Example 5: A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: 120g of kitchen waste that had been stored for 2 days was weighed and added to 1 L LB medium, and incubated overnight at 30℃ and 150 rpm with shaking. The obtained microbial community was dominated by Leuconostoc mesenteroides and Saccharomyces cerevisiae. After enrichment culture, the relative content of Leuconostoc mesenteroides at the bacterial level was 40%, and the relative content of Saccharomyces cerevisiae at the fungal level was 90%. The microbial cells were collected by centrifugation.

[0033] 2.455 g of manganese chloride was dissolved in 1000 mL of sterile water, denoted as solution A. Then, 100 mL of solution A was taken and 300 mL of 8% sodium hydroxide solution (solution B) was added, maintaining a volume ratio of B:A = 3. The mixture was stirred thoroughly with a magnetic stirrer, with 0.7 g of tannic acid added as a complexing agent, and allowed to react for 1 hour. After centrifugation, rinsing, and drying, nano-manganese oxide with catalytic properties was obtained. 8.0 g of nano-manganese oxide was dissolved in 2 LMOPs buffer solution, and then 0.6 g of microbial cells were added and mixed with the nano-manganese oxide solution, maintaining a microbial cell concentration of 0.1–6 g / L. The mixture was incubated at 20℃ and 100 rpm for 1 hour. The nano-manganese oxide adhered to the bacterial surface or bacterial appendages, spontaneously forming a microbial nano-manganese oxide composite ecological community. Centrifugation was then used to obtain the microbial nano-manganese oxide composite material.

[0034] In a 1 L container, 10.0 g of microbial nano-manganese oxide composite material and 1200 g of kitchen waste were added and thoroughly mixed. No acclimatization time was required; the mixture was stirred directly at 50 r / min, with the reaction temperature controlled at 15℃. After 4 hours of reaction, the kitchen waste weight reduction rate was 90%, and solid organic matter was converted into liquid organic acid metabolites. The metabolic liquid products BOD5 / COD ratio was [not specified]. Cr It is 0.75.

[0035] Example 6: A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: Weigh 250g of kitchen waste that has been stored for 2 days and add it to 1 L LB medium. Shake overnight at 30℃ and 150 rpm. The obtained microbial community is dominated by Leuconostoc mesenteroides and Saccharomyces cerevisiae. After enrichment culture, the relative content of Leuconostoc mesenteroides at the bacterial level is 43%, and the relative content of Saccharomyces cerevisiae at the fungal level is 93%. Collect the microbial cells by centrifugation.

[0036] 3.455 g of manganese chloride was dissolved in 1000 mL of sterile water, denoted as solution A. Then, 100 mL of solution A was taken and 300 mL of 8% sodium hydroxide solution (solution B) was added, maintaining a volume ratio of B:A = 3. The mixture was stirred thoroughly with a magnetic stirrer, with 0.5 g of tannic acid added as a complexing agent, and allowed to react for 1 hour. After centrifugation, rinsing, and drying, nano-manganese oxide with catalytic properties was obtained. 8.0 g of nano-manganese oxide was dissolved in 2 LMOPs buffer solution, and then 0.6 g of microbial cells were added and mixed with the nano-manganese oxide solution, maintaining a microbial cell concentration of 0.1–6 g / L. The mixture was incubated at 20℃ and 100 rpm for 1 hour. The nano-manganese oxide adhered to the bacterial surface or bacterial appendages, spontaneously forming a microbial nano-manganese oxide composite ecological community. Centrifugation was then used to obtain the microbial nano-manganese oxide composite material.

[0037] In a 1 L container, 10.0 g of microbial nano-manganese oxide composite material and 1200 g of kitchen waste were added and thoroughly mixed. No acclimatization time was required; the mixture was stirred directly at 35 r / min, with the reaction temperature controlled at 15℃. After 2 hours of reaction, the kitchen waste weight reduction rate was 90%, and solid organic matter was converted into liquid organic acid metabolites. The metabolic liquid products BOD5 / COD ratio was [not specified]. Cr It is 0.60.

[0038] Example 7: A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, comprising the following steps: 300g of kitchen waste that had been stored for 1 day was weighed and added to 1 L LB medium. The mixture was shaken overnight at 30°C and 150 rpm. The obtained microbial community was dominated by Leuconostoc mesenteroides and Saccharomyces cerevisiae. After enrichment culture, the relative content of Leuconostoc mesenteroides at the bacterial level was 38%, and the relative content of Saccharomyces cerevisiae at the fungal level was 88%. The microbial cells were collected by centrifugation.

[0039] 3.155 g of manganese chloride was dissolved in 1000 mL of sterile water, denoted as solution A. Then, 100 mL of solution A was taken and 300 mL of 10% sodium hydroxide solution (solution B) was added, maintaining a volume ratio of B:A = 3. The mixture was stirred thoroughly with a magnetic stirrer, with 0.8 g of tannic acid added as a complexing agent, and allowed to react for 1 hour. After centrifugation, rinsing, and drying, nano-manganese oxide with catalytic properties was obtained. 8.0 g of nano-manganese oxide was dissolved in 2 LMOPs buffer solution, and then 0.6 g of microbial cells were added and mixed with the nano-manganese oxide solution, maintaining a microbial cell concentration of 0.1–6 g / L. The mixture was incubated at 20℃ and 100 rpm for 1 hour. The nano-manganese oxide adhered to the bacterial surface or bacterial appendages, spontaneously forming a microbial nano-manganese oxide composite material ecological community. Centrifugation was then used to obtain the microbial nano-manganese oxide composite material.

[0040] In a 1 L container, 10.0 g of microbial nano-manganese oxide composite material and 1200 g of kitchen waste were added and thoroughly mixed. No acclimatization time was required; the mixture was stirred directly at 50 r / min, with the reaction temperature controlled at 20℃. After 2 hours of reaction, the kitchen waste weight reduction rate was 90%, and solid organic matter was converted into liquid organic acid metabolites. The metabolic liquid products BOD5 / COD ratio was [not specified]. Cr It is 0.70.

[0041] Those skilled in the art will understand that the above-described embodiments are specific examples of implementing this application, and in practical applications, various changes in form and detail may be made without departing from the spirit and scope of this application. Any person skilled in the art can make their own modifications and alterations without departing from the spirit and scope of this application; therefore, the scope of protection of this application should be determined by the scope defined in the claims.

Claims

1. A method for efficiently degrading kitchen waste using microbial nano-manganese oxide composite materials, characterized in that, Includes the following steps: Step (1) Enrichment and cultivation of microbial communities for degrading kitchen waste: Using kitchen waste / food waste as the inoculum source, continuous subculture and domestication are carried out using culture medium, followed by shaking and centrifugation to collect microbial cells; wherein, Leuconostoc mesenteroides and Saccharomyces cerevisiae are the dominant microbial genera in the microbial cells. Step (2) Preparation of microbial nano-manganese oxide composite material: Using manganese chloride as the manganese source, the manganese salt is dissolved in sterile water to obtain a manganese salt mixture solution; the manganese salt mixture solution is added to sodium hydroxide solution, magnetically stirred, and tannic acid is added as a complexing agent, and the reaction is carried out for 1~3 h; then centrifugation, rinsing and drying are performed to obtain nano-manganese oxide with catalytic properties; the nano-manganese oxide particles are dissolved in MOPs buffer solution to obtain nano-manganese oxide solution; the microbial cells are mixed with the nano-manganese oxide solution, the concentration of the microbial cells is controlled at 0.1 g / L~6 g / L, and incubated at 20℃ and 100 rpm for 2 h, and centrifuged to obtain microbial nano-manganese oxide composite material.

2. The method as described in claim 1, characterized in that, The inoculation source is specifically kitchen waste / food waste that has been stored for 1 to 2 days, with an inoculation amount of 30 g / L to 300 g / L, and the culture conditions are 20℃ to 40℃, with shaking parameters of 120 rpm to 200 rpm.

3. The method as described in claim 1, characterized in that, Among the microbial cells, Leuconostoc mesenteroides has a relative content of 35% to 45% at the bacterial level, and Saccharomyces cerevisiae has a relative content of 85% to 95% at the fungal level.

4. The method as described in claim 1, characterized in that, In step (2), the concentration of manganese chloride is 2 g / L to 5 g / L; the mass fraction of the sodium hydroxide solution is 5% to 10%; the volume ratio of the manganese salt mixture solution to the sodium hydroxide solution is 1:3; the mass fraction of tannic acid is 0.1% to 1%; and the nano-manganese oxide solution is prepared by dissolving 1 g to 10 g of the nano-manganese oxide in 2 LMOPs buffer solution.

5. The method as described in claim 1, characterized in that, In step (2), the ratio of nano-manganese oxide to microbial cells is 0.6 to 3.

5.

6. The method as described in claim 1, characterized in that, In step (2), the incubation parameters are: the concentration of the microbial cells is 0.1 g / L to 6 g / L, and the cells are incubated at 20℃ and 100 rpm for 2 h.

7. The microbial nano-manganese oxide composite material prepared by the method described in any one of claims 1 to 6.

8. The application of the microbial nano-manganese oxide composite material as described in claim 7 in the field of degrading kitchen waste / food waste.

9. The application according to claim 8, characterized in that, The degradation method for kitchen waste / food waste includes: The microbial nano-manganese oxide composite material is thoroughly mixed with kitchen waste / food waste, stirred at 30 r / min to 50 r / min, and the reaction temperature is controlled at 15℃ to 30℃. Under aerobic conditions, mechanical stirring is carried out, and the degradation is completed after 2 h to 4 h of reaction.

10. The application according to claim 8, characterized in that, The specific addition amounts are: 1 g / L to 12 g / L for the microbial nano-manganese oxide composite material, and 300 g / L to 1200 g / L for kitchen waste / food waste. The ratio of the microbial nano-manganese oxide composite material to kitchen waste / food waste is 1:(100~900). After the degradation process is complete, the weight reduction rate of kitchen waste / food waste is 85%~99%, and the metabolic liquid products BOD5 / COD ratio is [not specified]. Cr The value ranges from 0.55 to 0.88.