Water quality environment improver

Abstract

The invention discloses a water quality environment improver which comprises the following raw materials in percentage by mass: 35%-55% of plant active matters, 12%-22% of various photosynthetic bacteria, 8%-18% of bacillus subtilis, 8%-18% of bacillus megatherium, 3%-8% of marine alginate oligosaccharide, 0.5%-2% of humic acid chelated selenium and 2%-6% of bacillus amyloliquefaciens fermentation metabolites. Through triple effects of a microbial quantum process, algae photosynthesis and oligosaccharide bacterium promotion, dissolved oxygen in a water body can be increased to 6-8 mg / L from initial 3-4 mg / L, the maintenance time is as long as 96 h or more and is prolonged by 40%-60% compared with that of a traditional modifier, through cooperation of bacillus and bacillus amyloliquefaciens metabolic enzyme, the degradation rate of protein and starch organic matters reaches 55%-70%, and the degradation rate of the bacillus amyloliquefaciens metabolic enzyme reaches 55%-70%. The COD removal rate is increased by 25%-35% compared with that of a single strain preparation.

Description

Technical Field

[0001] This invention relates to the field of water purification technology, specifically to a water quality environment improver. Background Technology

[0002] With the large-scale development of aquaculture and the discharge of industrial and domestic wastewater, problems such as eutrophication, insufficient dissolved oxygen, and microecological imbalance in water bodies are becoming increasingly prominent. Excessive accumulation of organic matter in water bodies leads to a surge in oxygen consumption due to microbial oxidation and decomposition, causing fish to surface for air and die; at the same time, the proliferation of harmful algae (such as cyanobacteria) inhibits the growth of beneficial organisms and disrupts the aquatic ecosystem.

[0003] Currently, water quality improvers on the market are mainly divided into chemical, single-microbial, and plant extract types, and have the following shortcomings: Chemical improvers, such as alum and polyaluminum chloride, can quickly precipitate organic matter, but they produce a large amount of sludge, leading to the deterioration of the water body bottom; chemical oxygenators have a short duration of action and are prone to causing secondary pollution; Single-microbial improvers, such as products containing only photosynthetic bacteria, can decompose some organic matter, but their effect on increasing dissolved oxygen is limited; products containing only Bacillus require a large amount of oxygen, which may exacerbate the problem of insufficient dissolved oxygen; and single-species bacteria have poor stress resistance and are difficult to colonize in complex aquatic environments; Plant extract improvers, such as traditional Chinese medicine extracts, have a certain antibacterial effect, but they are expensive and have a weak effect on the degradation of organic matter and the increase of dissolved oxygen; Shortcomings of compound improvers: Although some existing compound improvers combine microorganisms and plant extracts, they lack the design of special functional components and fail to achieve the synergistic effect of "oxygenation-degradation-balance-stress resistance"; and the preparation process is crude, the microbial activity is low, and the product stability is poor. Summary of the Invention

[0004] The purpose of this invention is to provide a water quality improver to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a water quality environment improver, the raw material composition by mass percentage including 35%-55% plant active substances, 12%-22% various photosynthetic bacteria, 8%-18% Bacillus subtilis, 8%-18% Bacillus megaterium, 3%-8% marine brown algae oligosaccharides, 0.5%-2% humic acid chelated selenium, and 2%-6% fermentation metabolites of Bacillus amyloliquefaciens.

[0006] Optionally, the plurality of photosynthetic bacteria includes at least strains from the Rhodospirilluceae and Chromophyceae families, and the viable count of each strain is not less than 1×10⁻⁶. 9 CFU / g; the viable count of the Bacillus subtilis and Bacillus megaterium is not less than 5 × 10⁻⁶. 9The CFU / g of the marine brown algae oligosaccharide has undergone salt tolerance acclimation treatment. The oligosaccharide is produced by enzymatic hydrolysis, decolorization, desalination, and spray drying of brown algae. The molecular weight is 1000-3000 Da, the purity is ≥90%, and the fucose content is ≥25% and the mannuronic acid content is ≥30%.

[0007] Optionally, the humic acid chelated selenium is prepared by chelating humic acid extracted from weathered coal with sodium selenite at a mass ratio of 10:1-15:1, with a chelation rate ≥80%, a selenium content of 0.1%-0.3%, and a pH value of 5.5-7.0; the Bacillus amyloliquefaciens fermentation metabolite is prepared by Bacillus amyloliquefaciens through liquid fermentation, centrifugation, membrane filtration, and vacuum concentration, wherein the amylase activity is ≥500U / g, the antimicrobial peptide content is ≥1.2mg / g, and the protease activity is ≥300U / g.

[0008] Optionally, the plant active ingredient is a compound of legume extract and green algae extract, wherein the legume extract accounts for 60%-80% and the green algae extract accounts for 20%-40%.

[0009] Optionally, the preparation process of the legume plant extract is as follows: Select fresh whole plant of milkvetch or alfalfa, wash and crush to a particle size ≤5mm, add deionized water at a solid-liquid ratio of 1:8-1:12, extract at a constant temperature of 80-90℃ for 2-3 hours, stirring 3-5 times for 10 minutes each time, filter the extract through plate and frame filter to remove residue, concentrate under reduced pressure to a solid content ≥30%, and finally spray dry to make powder with a total flavonoid content ≥5% and a polysaccharide content ≥15%.

[0010] Optionally, the preparation process of the green algae extract is as follows: Chlorella or Scenedesmus algae mud is selected, and the cell walls are broken 2-3 times using a high-pressure homogenizer at a pressure of 40-50 MPa and a temperature of 25-30℃. After cell wall breaking, deionized water is added at a solid-liquid ratio of 1:5, and a compound protease is added. The mixture is enzymatically hydrolyzed at 50-55℃ and pH 6.5-7.0 for 1-2 hours. The hydrolysate is centrifuged to remove the precipitate, and the supernatant is vacuum concentrated to a solid content ≥25%. The mixture is then spray-dried to produce a powder with a chlorophyll content ≥1.2% and a total amino acid content ≥20%.

[0011] Optionally, the preparation method of the water quality environmental improver includes the following steps: S1: Raw material pretreatment—Plant active substances are pulverized and passed through an 80-100 mesh sieve; marine brown algae oligosaccharides are dissolved in sterile water (mass ratio 1:5-1:8) to prepare an oligosaccharide solution; humic acid chelated selenium is passed through a 200 mesh sieve for later use; various photosynthetic bacteria, Bacillus subtilis, Bacillus megaterium, and Bacillus amyloliquefaciens are inoculated into their respective seed culture media and cultured with shaking (180-200 r / min) for 18-24 h at 30-35℃ and pH 7.0-7.5 to obtain seed solutions of each bacteria; the Bacillus amyloliquefaciens seed solution is centrifuged (5000-6000 r / min, 10-15 min) and the supernatant is collected, which is the fermentation metabolite of Bacillus amyloliquefaciens; S2: Mixed fermentation – Plant active ingredient powder and fermentation medium are mixed at a mass ratio of 1:5-1:8, sterilized at 121℃ for 25-30 min, cooled to 30-35℃, and then sequentially inoculated with various photosynthetic bacteria seed liquids (35%-45%), Bacillus subtilis seed liquid (20%-25%), and Bacillus megaterium seed liquid (20%-25%). Fermentation is carried out at 32-34℃ in an alternating anaerobic-microaerobic environment (8h anaerobic - 4h microaerobic as one cycle, 3-4 cycles in total) for 48-60 h to obtain the basic fermentation product; S3: Compound formulation - Slowly add oligosaccharide solution, humic acid chelated selenium powder and Bacillus amyloliquefaciens fermentation metabolites to the basic fermentation product, stir at 25-30℃ (100-120r / min) for 30-45min, then mix with sterile water at a mass ratio of 1:1-1:2, and grind with a colloid mill until the particle size is ≤5μm; S4: Finished product preparation - Add food-grade preservative (ε-polylysine, 0.01%-0.03%) to the ground mixture, stir well, and then dispense (1000ml / bottle) to obtain the finished product.

[0012] Optionally, the seed culture medium for the various photosynthetic bacteria, Bacillus subtilis, and Bacillus megaterium described in S1 is composed of: 10-15 g / L protein powder, 20-30 g / L brown sugar powder, 5-8 g / L amino acids (glutamic acid: aspartic acid = 1:1), 1-2 g / L KH2PO4, and MgSO4. The seed culture medium for Bacillus amyloliquefaciens consists of: 15-20 g / L soluble starch, 5-8 g / L yeast extract, 3-5 g / L peptone, 0.8-1.2 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4; the fermentation culture medium described in S2 consists of: 5-8 g / L protein powder, 10-15 g / L brown sugar powder, 3-5 g / L yeast extract, 0.5-1 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. This invention utilizes a triple effect of microbial quantum processes, algal photosynthesis, and oligosaccharide-promoting bacteria to increase dissolved oxygen in water from an initial 3-4 mg / L to 6-8 mg / L, maintaining this level for over 96 hours, which is 40%-60% longer than traditional amendments. Through the synergistic effect of Bacillus and Bacillus amyloliquefaciens metabolic enzymes, the degradation rate of proteins and starchy organic matter reaches 55%-70%, and the COD removal rate is 25%-35% higher than single-strain preparations. Marine brown algae oligosaccharides promote the growth of beneficial algae, while antimicrobial peptides inhibit harmful algae, resulting in a beneficial algae content of 70%-80%, effectively preventing algal blooms. Humic acid chelates selenium to supplement trace elements, and combined with the antimicrobial peptide's antibacterial effect, reducing stress-induced mortality in aquatic animals by 30%-40% and disease incidence by 25%-30%.

[0014] 2. By adjusting the amount of humic acid chelated selenium added, this invention can be adapted to seawater, freshwater, and aquatic species with different sensitivities, and its compatibility is superior to traditional products. Detailed Implementation

[0015] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0016] Example 1 A water quality improver, the raw material composition by mass percentage includes 35% plant active ingredients, 12% various photosynthetic bacteria, 8% Bacillus subtilis, 8% Bacillus megaterium, 3% marine brown algae oligosaccharides, 0.5% humic acid chelated selenium, and 2% fermentation metabolites of Bacillus amyloliquefaciens.

[0017] Specifically, the aforementioned multiple photosynthetic bacteria include strains from the Rhodospirilluceae and Chromophyceae families, and the viable count of each strain is not less than 1×10⁻⁶. 9 CFU / g; the viable count of the Bacillus subtilis and Bacillus megaterium is not less than 5 × 10⁻⁶. 9 The CFU / g of the marine brown algae oligosaccharide has undergone salt tolerance acclimation treatment. The oligosaccharide is produced by enzymatic hydrolysis, decolorization, desalination, and spray drying of brown algae. The molecular weight is 1000-3000 Da, the purity is ≥90%, and the fucose content is ≥25% and the mannuronic acid content is ≥30%.

[0018] Specifically, the humic acid chelated selenium is prepared by chelating humic acid extracted from weathered coal with sodium selenite at a mass ratio of 10:1-15:1, with a chelation rate ≥80%, a selenium content of 0.1%-0.3%, and a pH value of 5.5-7.0; the Bacillus amyloliquefaciens fermentation metabolite is prepared by Bacillus amyloliquefaciens through liquid fermentation, centrifugation, membrane filtration, and vacuum concentration, wherein the amylase activity is ≥500U / g, the antimicrobial peptide content is ≥1.2mg / g, and the protease activity is ≥300U / g.

[0019] Specifically, the plant active ingredient is a compound of legume extract and green algae extract, wherein the legume extract accounts for 60%-80% and the green algae extract accounts for 20%-40%.

[0020] Specifically, the preparation process of the legume plant extract is as follows: Select fresh whole plant of milkvetch or alfalfa, wash and crush to a particle size ≤5mm, add deionized water at a solid-liquid ratio of 1:8-1:12, extract at a constant temperature of 80-90℃ for 2-3 hours, stirring 3-5 times for 10 minutes each time, filter the extract through plate and frame filter to remove residue, concentrate under reduced pressure to a solid content ≥30%, and finally spray dry to make powder with a total flavonoid content ≥5% and a polysaccharide content ≥15%.

[0021] Specifically, the preparation process of the green algae extract is as follows: Chlorella or Scenedesmus algae mud is selected, and the cell walls are broken 2-3 times using a high-pressure homogenizer at a pressure of 40-50 MPa and a temperature of 25-30℃. After cell wall breaking, deionized water is added at a solid-liquid ratio of 1:5, and a compound protease is added. The mixture is enzymatically hydrolyzed for 1-2 hours at 50-55℃ and pH 6.5-7.0. The hydrolysate is centrifuged to remove the precipitate, and the supernatant is vacuum concentrated to a solid content ≥25%. The mixture is then spray-dried to produce a powder with a chlorophyll content ≥1.2% and a total amino acid content ≥20%.

[0022] Specifically, the preparation method of the water quality environmental improver includes the following steps: S1: Raw material pretreatment—Plant active substances are pulverized and passed through an 80-100 mesh sieve; marine brown algae oligosaccharides are dissolved in sterile water (mass ratio 1:5-1:8) to prepare an oligosaccharide solution; humic acid chelated selenium is passed through a 200 mesh sieve for later use; various photosynthetic bacteria, Bacillus subtilis, Bacillus megaterium, and Bacillus amyloliquefaciens are inoculated into their respective seed culture media and cultured with shaking (180-200 r / min) for 18-24 h at 30-35℃ and pH 7.0-7.5 to obtain seed solutions of each bacteria; the Bacillus amyloliquefaciens seed solution is centrifuged (5000-6000 r / min, 10-15 min) and the supernatant is collected, which is the fermentation metabolite of Bacillus amyloliquefaciens; S2: Mixed fermentation – Plant active ingredient powder and fermentation medium are mixed at a mass ratio of 1:5-1:8, sterilized at 121℃ for 25-30 min, cooled to 30-35℃, and then sequentially inoculated with various photosynthetic bacteria seed liquids (35%-45%), Bacillus subtilis seed liquid (20%-25%), and Bacillus megaterium seed liquid (20%-25%). Fermentation is carried out at 32-34℃ in an alternating anaerobic-microaerobic environment (8h anaerobic - 4h microaerobic as one cycle, 3-4 cycles in total) for 48-60 h to obtain the basic fermentation product; S3: Compound formulation - Slowly add oligosaccharide solution, humic acid chelated selenium powder and Bacillus amyloliquefaciens fermentation metabolites to the basic fermentation product, stir at 25-30℃ (100-120r / min) for 30-45min, then mix with sterile water at a mass ratio of 1:1-1:2, and grind with a colloid mill until the particle size is ≤5μm; S4: Finished product preparation - Add food-grade preservative (ε-polylysine, 0.01%-0.03%) to the ground mixture, stir well, and then dispense (1000ml / bottle) to obtain the finished product.

[0023] Specifically, the seed culture medium for the various photosynthetic bacteria, Bacillus subtilis, and Bacillus megaterium described in S1 consists of: 10-15 g / L protein powder, 20-30 g / L brown sugar powder, 5-8 g / L amino acids (glutamic acid: aspartic acid = 1:1), 1-2 g / L KH2PO4, and MgSO4. The seed culture medium for Bacillus amyloliquefaciens consists of: 15-20 g / L soluble starch, 5-8 g / L yeast extract, 3-5 g / L peptone, 0.8-1.2 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4; the fermentation culture medium described in S2 consists of: 5-8 g / L protein powder, 10-15 g / L brown sugar powder, 3-5 g / L yeast extract, 0.5-1 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4.

[0024] Finished product test results: Viable bacteria count: 6.2 × 10⁻⁶ 9 CFU / mL; pH: 7.0; Particle size: ≤5μm (pass rate 99.2%); Heavy metals: Pb 0.08mg / kg, Cd 0.03mg / kg, As 0.04mg / kg, meeting the standards.

[0025] Example 2 A water quality improver, the raw material composition by mass percentage includes 55% plant active ingredients, 22% various photosynthetic bacteria, 18% Bacillus subtilis, 18% Bacillus megaterium, 8% marine brown algae oligosaccharides, 2% humic acid chelated selenium, and 6% fermentation metabolites of Bacillus amyloliquefaciens.

[0026] Specifically, the aforementioned multiple photosynthetic bacteria include strains from the Rhodospirilluceae and Chromophyceae families, and the viable count of each strain is not less than 1×10⁻⁶. 9 CFU / g; the viable count of the Bacillus subtilis and Bacillus megaterium is not less than 5 × 10⁻⁶. 9 The CFU / g of the marine brown algae oligosaccharide has undergone salt tolerance acclimation treatment. The oligosaccharide is produced by enzymatic hydrolysis, decolorization, desalination, and spray drying of brown algae. The molecular weight is 1000-3000 Da, the purity is ≥90%, and the fucose content is ≥25% and the mannuronic acid content is ≥30%.

[0027] Specifically, the humic acid chelated selenium is prepared by chelating humic acid extracted from weathered coal with sodium selenite at a mass ratio of 10:1-15:1, with a chelation rate ≥80%, a selenium content of 0.1%-0.3%, and a pH value of 5.5-7.0; the Bacillus amyloliquefaciens fermentation metabolite is prepared by Bacillus amyloliquefaciens through liquid fermentation, centrifugation, membrane filtration, and vacuum concentration, wherein the amylase activity is ≥500U / g, the antimicrobial peptide content is ≥1.2mg / g, and the protease activity is ≥300U / g.

[0028] Specifically, the plant active ingredient is a compound of legume extract and green algae extract, wherein the legume extract accounts for 60%-80% and the green algae extract accounts for 20%-40%.

[0029] Specifically, the preparation process of the legume plant extract is as follows: Select fresh whole plant of milkvetch or alfalfa, wash and crush to a particle size ≤5mm, add deionized water at a solid-liquid ratio of 1:8-1:12, extract at a constant temperature of 80-90℃ for 2-3 hours, stirring 3-5 times for 10 minutes each time, filter the extract through plate and frame filter to remove residue, concentrate under reduced pressure to a solid content ≥30%, and finally spray dry to make powder with a total flavonoid content ≥5% and a polysaccharide content ≥15%.

[0030] Specifically, the preparation process of the green algae extract is as follows: Chlorella or Scenedesmus algae mud is selected, and the cell walls are broken 2-3 times using a high-pressure homogenizer at a pressure of 40-50 MPa and a temperature of 25-30℃. After cell wall breaking, deionized water is added at a solid-liquid ratio of 1:5, and a compound protease is added. The mixture is enzymatically hydrolyzed for 1-2 hours at 50-55℃ and pH 6.5-7.0. The hydrolysate is centrifuged to remove the precipitate, and the supernatant is vacuum concentrated to a solid content ≥25%. The mixture is then spray-dried to produce a powder with a chlorophyll content ≥1.2% and a total amino acid content ≥20%.

[0031] Specifically, the preparation method of the water quality environmental improver includes the following steps: S1: Raw material pretreatment—Plant active substances are pulverized and passed through an 80-100 mesh sieve; marine brown algae oligosaccharides are dissolved in sterile water (mass ratio 1:5-1:8) to prepare an oligosaccharide solution; humic acid chelated selenium is passed through a 200 mesh sieve for later use; various photosynthetic bacteria, Bacillus subtilis, Bacillus megaterium, and Bacillus amyloliquefaciens are inoculated into their respective seed culture media and cultured with shaking (180-200 r / min) for 18-24 h at 30-35℃ and pH 7.0-7.5 to obtain seed solutions of each bacteria; the Bacillus amyloliquefaciens seed solution is centrifuged (5000-6000 r / min, 10-15 min) and the supernatant is collected, which is the fermentation metabolite of Bacillus amyloliquefaciens; S2: Mixed fermentation – Plant active ingredient powder and fermentation medium are mixed at a mass ratio of 1:5-1:8, sterilized at 121℃ for 25-30 min, cooled to 30-35℃, and then sequentially inoculated with various photosynthetic bacteria seed liquids (35%-45%), Bacillus subtilis seed liquid (20%-25%), and Bacillus megaterium seed liquid (20%-25%). Fermentation is carried out at 32-34℃ in an alternating anaerobic-microaerobic environment (8h anaerobic - 4h microaerobic as one cycle, 3-4 cycles in total) for 48-60 h to obtain the basic fermentation product; S3: Compound formulation - Slowly add oligosaccharide solution, humic acid chelated selenium powder and Bacillus amyloliquefaciens fermentation metabolites to the basic fermentation product, stir at 25-30℃ (100-120r / min) for 30-45min, then mix with sterile water at a mass ratio of 1:1-1:2, and grind with a colloid mill until the particle size is ≤5μm; S4: Finished product preparation - Add food-grade preservative (ε-polylysine, 0.01%-0.03%) to the ground mixture, stir well, and then dispense (1000ml / bottle) to obtain the finished product.

[0032] Specifically, the seed culture medium for the various photosynthetic bacteria, Bacillus subtilis, and Bacillus megaterium described in S1 consists of: 10-15 g / L protein powder, 20-30 g / L brown sugar powder, 5-8 g / L amino acids (glutamic acid: aspartic acid = 1:1), 1-2 g / L KH2PO4, and MgSO4. The seed culture medium for Bacillus amyloliquefaciens consists of: 15-20 g / L soluble starch, 5-8 g / L yeast extract, 3-5 g / L peptone, 0.8-1.2 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4; the fermentation culture medium described in S2 consists of: 5-8 g / L protein powder, 10-15 g / L brown sugar powder, 3-5 g / L yeast extract, 0.5-1 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4.

[0033] Finished product test results: Viable bacteria count: 5×10 9 CFU / mL; pH: 6.2; Particle size: ≤7μm (pass rate 95.4%); Heavy metals: Pb 0.05mg / kg, Cd 0.02mg / kg, As 0.07mg / kg, in compliance with standards.

[0034] Example 3 A water quality improver, the raw material composition by mass percentage includes 45% plant active ingredients, 18% various photosynthetic bacteria, 13% Bacillus subtilis, 13% Bacillus megaterium, 5% marine brown algae oligosaccharides, 1% humic acid chelated selenium, and 4% fermentation metabolites of Bacillus amyloliquefaciens.

[0035] Specifically, the aforementioned multiple photosynthetic bacteria include strains from the Rhodospirilluceae and Chromophyceae families, and the viable count of each strain is not less than 1×10⁻⁶. 9 CFU / g; the viable count of the Bacillus subtilis and Bacillus megaterium is not less than 5 × 10⁻⁶. 9The CFU / g of the marine brown algae oligosaccharide has undergone salt tolerance acclimation treatment. The oligosaccharide is produced by enzymatic hydrolysis, decolorization, desalination, and spray drying of brown algae. The molecular weight is 1000-3000 Da, the purity is ≥90%, and the fucose content is ≥25% and the mannuronic acid content is ≥30%.

[0036] Specifically, the humic acid chelated selenium is prepared by chelating humic acid extracted from weathered coal with sodium selenite at a mass ratio of 10:1-15:1, with a chelation rate ≥80%, a selenium content of 0.1%-0.3%, and a pH value of 5.5-7.0; the Bacillus amyloliquefaciens fermentation metabolite is prepared by Bacillus amyloliquefaciens through liquid fermentation, centrifugation, membrane filtration, and vacuum concentration, wherein the amylase activity is ≥500U / g, the antimicrobial peptide content is ≥1.2mg / g, and the protease activity is ≥300U / g.

[0037] Specifically, the plant active ingredient is a compound of legume extract and green algae extract, wherein the legume extract accounts for 60%-80% and the green algae extract accounts for 20%-40%.

[0038] Specifically, the preparation process of the legume plant extract is as follows: Select fresh whole plant of milkvetch or alfalfa, wash and crush to a particle size ≤5mm, add deionized water at a solid-liquid ratio of 1:8-1:12, extract at a constant temperature of 80-90℃ for 2-3 hours, stirring 3-5 times for 10 minutes each time, filter the extract through plate and frame filter to remove residue, concentrate under reduced pressure to a solid content ≥30%, and finally spray dry to make powder with a total flavonoid content ≥5% and a polysaccharide content ≥15%.

[0039] Specifically, the preparation process of the green algae extract is as follows: Chlorella or Scenedesmus algae mud is selected, and the cell walls are broken 2-3 times using a high-pressure homogenizer at a pressure of 40-50 MPa and a temperature of 25-30℃. After cell wall breaking, deionized water is added at a solid-liquid ratio of 1:5, and a compound protease is added. The mixture is enzymatically hydrolyzed for 1-2 hours at 50-55℃ and pH 6.5-7.0. The hydrolysate is centrifuged to remove the precipitate, and the supernatant is vacuum concentrated to a solid content ≥25%. The mixture is then spray-dried to produce a powder with a chlorophyll content ≥1.2% and a total amino acid content ≥20%.

[0040] Specifically, the preparation method of the water quality environmental improver includes the following steps: S1: Raw material pretreatment—Plant active substances are pulverized and passed through an 80-100 mesh sieve; marine brown algae oligosaccharides are dissolved in sterile water (mass ratio 1:5-1:8) to prepare an oligosaccharide solution; humic acid chelated selenium is passed through a 200 mesh sieve for later use; various photosynthetic bacteria, Bacillus subtilis, Bacillus megaterium, and Bacillus amyloliquefaciens are inoculated into their respective seed culture media and cultured with shaking (180-200 r / min) for 18-24 h at 30-35℃ and pH 7.0-7.5 to obtain seed solutions of each bacteria; the Bacillus amyloliquefaciens seed solution is centrifuged (5000-6000 r / min, 10-15 min) and the supernatant is collected, which is the fermentation metabolite of Bacillus amyloliquefaciens; S2: Mixed fermentation – Plant active ingredient powder and fermentation medium are mixed at a mass ratio of 1:5-1:8, sterilized at 121℃ for 25-30 min, cooled to 30-35℃, and then sequentially inoculated with various photosynthetic bacteria seed liquids (35%-45%), Bacillus subtilis seed liquid (20%-25%), and Bacillus megaterium seed liquid (20%-25%). Fermentation is carried out at 32-34℃ in an alternating anaerobic-microaerobic environment (8h anaerobic - 4h microaerobic as one cycle, 3-4 cycles in total) for 48-60 h to obtain the basic fermentation product; S3: Compound formulation - Slowly add oligosaccharide solution, humic acid chelated selenium powder and Bacillus amyloliquefaciens fermentation metabolites to the basic fermentation product, stir at 25-30℃ (100-120r / min) for 30-45min, then mix with sterile water at a mass ratio of 1:1-1:2, and grind with a colloid mill until the particle size is ≤5μm; S4: Finished product preparation - Add food-grade preservative (ε-polylysine, 0.01%-0.03%) to the ground mixture, stir well, and then dispense (1000ml / bottle) to obtain the finished product.

[0041] Specifically, the seed culture medium for the various photosynthetic bacteria, Bacillus subtilis, and Bacillus megaterium described in S1 consists of: 10-15 g / L protein powder, 20-30 g / L brown sugar powder, 5-8 g / L amino acids (glutamic acid: aspartic acid = 1:1), 1-2 g / L KH2PO4, and MgSO4. The seed culture medium for Bacillus amyloliquefaciens consists of: 15-20 g / L soluble starch, 5-8 g / L yeast extract, 3-5 g / L peptone, 0.8-1.2 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4; the fermentation culture medium described in S2 consists of: 5-8 g / L protein powder, 10-15 g / L brown sugar powder, 3-5 g / L yeast extract, 0.5-1 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4.

[0042] Finished product test results: Viable bacteria count: 9×10 9 CFU / mL; pH: 6.4; Particle size: ≤4.8μm (pass rate 97.5%); Heavy metals: Pb 0.09mg / kg, Cd 0.05mg / kg, As 0.07mg / kg, in compliance with standards.

[0043] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A water quality conditioner, characterized in that, The raw material composition, by mass percentage, includes 35%-55% plant active ingredients, 12%-22% various photosynthetic bacteria, 8%-18% Bacillus subtilis, 8%-18% Bacillus megaterium, 3%-8% marine brown algae oligosaccharides, 0.5%-2% humic acid chelated selenium, and 2%-6% fermentation metabolites of Bacillus amyloliquefaciens.

2. The water quality improver according to claim 1, characterized in that: The aforementioned photosynthetic bacteria include strains from at least the Rhodospirilluceae and Chromophyceae families, and the viable count of each strain is not less than 1 × 10⁻⁶. 9 CFU / g; the viable count of the Bacillus subtilis and Bacillus megaterium is not less than 5 × 10⁻⁶. 9 The CFU / g of the marine brown algae oligosaccharide is prepared by enzymatic hydrolysis, decolorization, desalination, and spray drying of brown algae. The molecular weight is 1000-3000 Da, the purity is ≥90%, and the fucose content is ≥25% and the mannuronic acid content is ≥30%.

3. The water quality improver according to claim 2, characterized in that: The humic acid chelated selenium is prepared by chelating humic acid extracted from weathered coal with sodium selenite at a mass ratio of 10:1-15:1, with a chelation rate ≥80%, a selenium content of 0.1%-0.3%, and a pH value of 5.5-7.0; the Bacillus amyloliquefaciens fermentation metabolite is prepared by Bacillus amyloliquefaciens through liquid fermentation, centrifugation, membrane filtration, and vacuum concentration, wherein the amylase activity is ≥500U / g, the antimicrobial peptide content is ≥1.2mg / g, and the protease activity is ≥300U / g.

4. The water quality improver according to claim 3, characterized in that: The plant active ingredient is a compound of legume extract and green algae extract, wherein the legume extract accounts for 60%-80% and the green algae extract accounts for 20%-40%.

5. The water quality improver according to claim 4, characterized in that: The preparation process of the legume plant extract is as follows: Select fresh whole plant of milkvetch or alfalfa, wash and crush to a particle size ≤5mm, add deionized water at a solid-liquid ratio of 1:8-1:12, extract at a constant temperature of 80-90℃ for 2-3 hours, stirring 3-5 times for 10 minutes each time, filter the extract through plate and frame filter to remove residue, concentrate under reduced pressure to a solid content ≥30%, and finally spray dry to make powder with a total flavonoid content ≥5% and a polysaccharide content ≥15%.

6. The water quality improver according to claim 5, characterized in that: The preparation process of the green algae extract is as follows: Chlorella or Scenedesmus algae mud is selected, and the cell walls are broken 2-3 times using a high-pressure homogenizer at a pressure of 40-50 MPa and a temperature of 25-30℃. After cell wall breaking, deionized water is added at a solid-liquid ratio of 1:5, and a compound protease is added. The mixture is enzymatically hydrolyzed for 1-2 hours at 50-55℃ and pH 6.5-7.

0. The hydrolysate is centrifuged to remove the precipitate, and the supernatant is vacuum concentrated to a solid content of ≥25%. The mixture is then spray-dried to produce a powder with a chlorophyll content of ≥1.2% and a total amino acid content of ≥20%.

7. The water quality improver according to any one of claims 1-6, characterized in that: The preparation method of the water quality environmental improver includes the following steps: S1: Raw material pretreatment—Plant active substances are pulverized and passed through an 80-100 mesh sieve; marine brown algae oligosaccharides are dissolved in sterile water (mass ratio 1:5-1:8) to prepare an oligosaccharide solution; humic acid chelated selenium is passed through a 200 mesh sieve for later use; various photosynthetic bacteria, Bacillus subtilis, Bacillus megaterium, and Bacillus amyloliquefaciens are inoculated into their respective seed culture media and cultured with shaking (180-200 r / min) for 18-24 h at 30-35℃ and pH 7.0-7.5 to obtain seed solutions of each bacteria; the Bacillus amyloliquefaciens seed solution is centrifuged (5000-6000 r / min, 10-15 min) and the supernatant is collected, which is the fermentation metabolite of Bacillus amyloliquefaciens; S2: Mixed fermentation – Plant active ingredient powder and fermentation medium are mixed at a mass ratio of 1:5-1:8, sterilized at 121℃ for 25-30 min, cooled to 30-35℃, and then sequentially inoculated with various photosynthetic bacteria seed liquids (35%-45%), Bacillus subtilis seed liquid (20%-25%), and Bacillus megaterium seed liquid (20%-25%). Fermentation is carried out at 32-34℃ in an alternating anaerobic-microaerobic environment (8h anaerobic - 4h microaerobic as one cycle, 3-4 cycles in total) for 48-60 h to obtain the basic fermentation product; S3: Compound formulation - Slowly add oligosaccharide solution, humic acid chelated selenium powder and Bacillus amyloliquefaciens fermentation metabolites to the basic fermentation product, stir at 25-30℃ (100-120r / min) for 30-45min, then mix with sterile water at a mass ratio of 1:1-1:2, and grind with a colloid mill until the particle size is ≤5μm; S4: Finished product preparation - Add food-grade preservative (ε-polylysine, 0.01%-0.03%) to the ground mixture, stir well, and then dispense (1000ml / bottle) to obtain the finished product. The water quality improver according to claim 1 is characterized in that: The seed culture medium for the various photosynthetic bacteria, Bacillus subtilis, and Bacillus megaterium described in S1 consists of: protein powder 10-15 g / L, brown sugar powder 20-30 g / L, amino acids (glutamic acid: aspartic acid = 1:1) 5-8 g / L, KH2PO4 1-2 g / L, and MgSO4. The seed culture medium for Bacillus amyloliquefaciens consists of: 15-20 g / L soluble starch, 5-8 g / L yeast extract, 3-5 g / L peptone, 0.8-1.2 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4; the fermentation culture medium described in S2 consists of: 5-8 g / L protein powder, 10-15 g / L brown sugar powder, 3-5 g / L yeast extract, 0.5-1 g / L KH2PO4, 0.3-0.5 g / L CaCl2, and pH 7.2-7.4.