Method and system for behavior domestication of mandarin fish fry in a seven-day cycle factory circulating water

Abstract

The application provides a seven-day period factory cyclic Megalobrama amblycephala fry behavior domestication method and system, relates to the Megalobrama amblycephala breeding technical field, and includes: domesticating the fry into a domestication pond on the first day; starting vertical water flow impact before feeding fresh bait fish according to a preset first feeding frequency on the second day; feeding electric shock disabled live bait according to a preset second feeding frequency on the third day; feeding mixed live bait according to a preset third feeding frequency on the fourth day; feeding mosaic bait according to a preset fourth feeding frequency on the fifth day; feeding pure artificial compound feed using a composite feeding mode and starting water flow impact on the sixth day; feeding pure artificial compound feed on the seventh day, and screening domestication successful individuals based on behavior indexes after the period ends, so that the Megalobrama amblycephala is rapidly domesticated from feeding live bait to feeding artificial compound feed.

Description

Technical Field

[0001] This invention relates to the field of mandarin fish farming technology, and in particular to a seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry. Background Technology

[0002] The mandarin fish farming industry has long been hampered by the core bottleneck of seedling domestication. Although various technical solutions have been attempted to solve the problem of transitioning mandarin fish to formulated feed, existing solutions generally have significant shortcomings: they either extend the domestication period to more than 10 to 15 days, or rely excessively on exogenous additives, or fail to meet the stringent requirements of modern recirculating aquaculture plants in terms of domestication efficiency and success rate.

[0003] For example, the invention patent with publication number CN117530210A discloses a method for domesticating mandarin fish fry, which is a gradual transition method. The mandarin fish fry are put into the breeding equipment and fed live bait for 24 days. After the mandarin fish fry in the breeding equipment are stable, dead bait fish are introduced into the breeding equipment. On the second day, the powdered feed and dead bait fish are mixed and the mixed material is put into the breeding equipment. This process is continued for 25 days. After the powdered feed and dead bait fish are finished, the pelleted feed and dead bait fish are mixed and the mixed material is put into the breeding equipment. This process is continued for 23 days.

[0004] For example, the invention patent with publication number CN120036450A discloses a domestication feed and domestication method for mandarin fish fry, which is an attractant-assisted method. The domestication feed for mandarin fish fry includes live crucian carp fry and silver carp fry, half-dead crucian carp fry and half-dead silver carp fry, mandarin fish powder, dead crucian carp fry and dead silver carp fry, and mandarin fish feed No. 0; an attractant is added to the powder, which is one of squid paste, betaine and black soldier fly larvae slurry.

[0005] For example, the invention patent with publication number CN118947606A discloses a mandarin fish acclimatization pond designed as a special acclimatization pond, which includes a pond body, an oxygenation device, an inlet, and an outlet. Below the inlet, there is a bait fish breeding frame, and a feeding device is set on the side of the bait fish breeding frame. The feeding device includes a feeding pipe, a bait storage tank set at the upper end of the feeding pipe, a feed hopper set at the upper end of the bait storage tank, and a cathode ring and an anode ring set inside the feeding pipe. The cathode ring and the anode ring are connected by wires and equipped with a pulse generator and a pulse power amplifier.

[0006] The core advantages of factory-style recirculating aquaculture lie in its controllable environment and efficient turnover. A acclimatization period of up to 15 days directly leads to decreased utilization of aquaculture facilities, slower capital turnover, and a significant increase in production costs. Therefore, the industry urgently needs an ultra-short-cycle acclimatization technology that can compress the acclimatization time to less than a week while maintaining high success and survival rates.

[0007] To achieve acclimatization within 7 days, the following major technical challenges must be overcome:

[0008] 1. The time window for behavior solidification is extremely narrow: the establishment of fish behavior habits depends on repeated reinforcement. The 7-day cycle means that clear behavioral progress must be completed every day, and the margin for error is extremely low.

[0009] 2. Significantly increased stress risk: High-intensity, fast-paced artificial intervention can easily cause fish fry to suffer excessive physiological and psychological stress, leading to decreased immunity, increased cannibalistic behavior, and increased mortality.

[0010] 3. The "steep slope effect" of feed transition is prominent: from live bait to feed, the differences in its movement characteristics, shape, texture and smell are huge. Crossing this gap in a very short time can easily trigger a group's refusal to eat.

[0011] 4. Individual differences are significantly amplified: In the short term, differences in individual learning abilities will further exacerbate the differentiation of feeding behavior in the group, affecting the overall domestication success rate.

[0012] Existing technologies have failed to provide a systematic solution to the aforementioned challenges. For example, the gradual transition method is slow and difficult to shorten the cycle; attractant methods can only mask the problem to a certain extent and do not strengthen the change in the fish's own feeding behavior; existing facility improvements mainly focus on environmental control and fail to organically combine with high-intensity behavioral training. In summary, there is an urgent need to develop a completely new methodology and technical system. This system not only needs to redesign the feed transition sequence to make it operable even with steep progress; it also needs to introduce more intensive behavioral regulation methods to accelerate the formation of conditioned reflexes; and it needs to build an intelligent control system that can monitor in real time, provide immediate feedback, and dynamically adjust to ensure that this "high-speed acclimatization" process always proceeds efficiently along the predetermined track. This is precisely the core technical problem that this invention aims to solve. Summary of the Invention

[0013] In view of this, embodiments of the present invention provide a seven-day cycle factory-scale recirculating aquaculture system for the behavioral domestication of mandarin fish fry, which can realize the rapid behavioral domestication of mandarin fish fry from feeding on live bait to feeding on artificial compound feed.

[0014] The technical solution of this invention is implemented as follows: On the first day of domestication, fish fry are introduced into the domestication pond; on the second day of domestication, live bait fish are fed in combination with vertical water flow and specific sound wave stimulation to induce the establishment of conditioned reflexes; on the third day of domestication, live bait that has been treated with low-voltage pulse electric shock is fed; on the fourth day of domestication, a mixture of mechanically damaged live bait and dead bait is fed; on the fifth day of domestication, artificial feed with dead bait embedded in it is fed; on the sixth day of domestication, pure artificial feed is fed under stimulation; on the seventh day of domestication, pure artificial feed is continued to be fed and individuals that have been successfully domesticated are selected based on behavioral indicators.

[0015] This invention provides a seven-day cycle method for the behavioral domestication of mandarin fish fry in a recirculating aquaculture system. The method includes: S1: On the first day of domestication, mandarin fish fry are placed into a domestication pond with preset environmental conditions in a recirculating aquaculture workshop; S2: On the second day of domestication, live bait fish are fed to the mandarin fish fry at a preset first feeding frequency, and a vertical water flow impact mechanism is activated before each feeding to induce the mandarin fish fry to establish a stimulus aggregation conditioned reflex; S3: On the third day of domestication, live bait fish that have been electrocuted are fed to the mandarin fish fry at a preset second feeding frequency to maintain high intensity S4: On the fourth day of domestication, the mandarin fish fry are fed mixed live bait at the preset third feeding frequency; S5: On the fifth day of domestication, the mandarin fish fry are fed embedded bait at the preset fourth feeding frequency; S6: On the sixth day of domestication, a compound feeding mode is adopted, and the mandarin fish fry are fed pure artificial compound feed. Before each feeding, a vertical water flow impact mechanism is activated; S7: On the seventh day of domestication, the mandarin fish fry are fed pure artificial compound feed at the preset fifth feeding frequency. After the cycle ends, individuals that have been successfully domesticated are dynamically selected based on their individual behavioral indicators.

[0016] This application also provides a seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry. This system is applied to a seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry. The system includes: an environmental control module, a feeding stimulation module, and an enhanced intelligent monitoring and real-time decision-making module. The environmental control module controls the environment of the recirculating tank at preset environmental conditions and is equipped with a local rapid heating device. The feeding stimulation module includes a precise feed state control unit and a multimodal stimulation delivery unit. The precise feed state control unit integrates a low-voltage pulse electric shock device, a mechanical damage device, and a feed embedding device, enabling batch preparation of feed in the required state according to a daily plan. The multimodal stimulation delivery unit provides focused light spot stimulation at the beginning of feeding. The enhanced intelligent monitoring and real-time decision-making module includes a high-frequency data acquisition system, an edge computing server, and real-time control software. It calculates the feeding attack speed index, the estimated daily feeding rate, and the conditioned reflex intensity index in real time, and generates an adjustment plan when deviations occur from the daily target.

[0017] The beneficial effects of the technical solutions provided in the embodiments of the present invention include at least the following:

[0018] 1. By implementing a seven-day phased precision domestication strategy, combined with multimodal stimuli such as vertical water flow impact, gradient transition of feed state, dynamic adjustment mechanism of release density and feeding parameters, and a supporting system of high-precision environmental control and intelligent monitoring and decision-making, the acceptance process of mandarin fish fry to artificial compound feed is accelerated, water quality fluctuations and fish conflicts are reduced, and the controllability, stability and efficiency of the domestication process are improved. This enables efficient and large-scale behavioral domestication of mandarin fish fry under factory recirculating water conditions, ensuring the success rate of domestication and the uniformity of fry size, and reducing the feed cost and management difficulty of factory farming.

[0019] 2. By implementing a seven-day phased precision domestication strategy, employing high-density stocking, small-body unitized sewage discharge design, and fry starvation treatment, combined with a composite stimulation mechanism consisting of vertical water flow and specific spectrum sound waves, and dynamically adjusting the stimulation intensity based on the fish's conditioned reflex intensity, further adjusting the stimulation duration when the stimulation intensity reaches a threshold but still does not meet the standard, the fish's conditioned reflex to the feeding signal is quickly established. This breaks the mandarin fish's inherent dependence on normally swimming prey, reduces the impact of water quality fluctuations on domestication, and improves the adaptability and effectiveness of the stimulation mechanism. As a result, the behavior domestication of mandarin fish fry under factory-style recirculating water conditions is efficiently promoted, the domestication cycle is shortened, and the controllability of the domestication process is enhanced, providing strong support for the subsequent adaptation of mandarin fish fry to artificial compound feed and improving the domestication success rate.

[0020] 3. By employing a dynamic adjustment mechanism for the electrode spacing based on the body shape and field strength distribution of the bait fish, and a dynamic adjustment mechanism for the initial stimulation intensity based on the fish species and their physiological activity state after energization, the low-voltage pulse electric shock device can accurately match the electric shock parameters to the body shape and species of different bait fish, ensuring the uniformity of the electric field distribution, effectively controlling the incapacitated state of the bait fish and ensuring their survival rate, avoiding excessive or insufficient electric shock, and thus achieving precise, stable and controllable bait state during the domestication of mandarin fish fry, providing reliable support for the smooth transition of bait morphology, and improving the stability and success rate of the domestication process.

[0021] 4. By using the aggressive behavior of mandarin fish fry towards pure artificial feed, their concentrated response to vertical water flow impact feeding signals, and their feed intake as core behavioral indicators, a three-step progressive dynamic screening method is adopted to accurately and objectively determine the individual domestication effect, distinguish between successfully domesticated and unsuccessfully domesticated fry, ensure uniform and quantifiable screening standards, and thus achieve scientific evaluation of the domestication effect of mandarin fish fry and efficient screening of high-quality domesticated fry, providing fry with uniform size and stable feeding for subsequent large-scale aquaculture.

[0022] 5. By establishing a dynamic adjustment mechanism for seedling stocking density based on domestication success rate and fish conflict frequency as core judgment criteria, and combining a density adjustment mapping table with a maximum safe density threshold, the seedling stocking density can be optimized reasonably. This avoids fish conflicts caused by excessive density or water utilization rate reduction due to excessive density, ensuring a stable and controllable domestication process. As a result, the efficiency of mandarin fish seedling domestication, unit water utilization rate and domestication success rate are improved simultaneously, while ensuring the safety of the aquaculture process.

[0023] 6. By constructing feeding assessment indicators quantified by the feeding attack speed index and the estimated daily feeding rate, and combining them with a dynamic adjustment mechanism for feeding control parameters using a feed adjustment mapping table and a feeding time adjustment mapping table, the feeding type ratio and feeding time interval are dynamically adjusted in real time based on the deviation of the feeding assessment indicators. This allows for precise adaptation to the feeding status of mandarin fish fry at each domestication stage, timely correction of domestication deviations, and avoidance of feeding abnormalities caused by excessively rapid feed transitions or unreasonable feeding rhythms. Consequently, refined and intelligent control of feeding strategies is achieved, improving the stability of the domestication process and the feeding efficiency of fry, and ensuring the domestication effect and success rate of mandarin fish fry. Attached Figure Description

[0024] Figure 1 This is a flowchart of a seven-day cycle factory-scale recirculating aquaculture system for the behavioral domestication of mandarin fish fry, provided by an embodiment of the present invention.

[0025] Figure 2 This is a schematic diagram of the structure of the seven-day cycle factory-scale recirculating aquatic mandarin fish fry behavior domestication system provided in an embodiment of the present invention. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application will be described in further detail below with reference to the accompanying drawings.

[0027] In this application, the terms "first," "second," "third," etc., are used to distinguish identical or similar items with substantially the same function and purpose. It should be understood that there is no logical or temporal dependency between "first," "second," and "nth," nor does it limit the quantity or execution order. It should also be understood that although the following description uses the terms "first," "second," etc., to describe various elements, these elements should not be limited by the terms. These terms are merely used to distinguish one element from another. For example, "first device," "second device," "third device," etc., are only used to distinguish devices. Similarly, "first sample data," "second sample data," and "third sample data," etc., are only used to distinguish sample data. Without departing from the scope of the various examples, a first device can be referred to as a second device, and similarly, a second device can be referred to as a first device. Both the first device and the second device are devices, and in some cases, they can be separate and distinct devices.

[0028] It should be understood that the terminology used in the description of the various examples herein is for the purpose of describing the particular examples only and is not intended to be limiting. As used in the description of the various examples and in the appended claims, the singular forms “a” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise.

[0029] It should also be understood that the term "and / or" as used herein refers to and covers any and all possible combinations of one or more of the associated listed items. The term "and / or" describes an association between related objects, indicating that three relationships can exist; for example, A and / or B can represent: A alone, A and B simultaneously, and B alone. Additionally, the character " / " in this application generally indicates that the preceding and following related objects are in an "or" relationship.

[0030] It should also be understood that, in the various embodiments of this application, the sequence number of each process does not imply the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0031] It should also be understood that determining B based on A does not mean determining B solely based on A; it is also possible to determine B based on A and / or other information.

[0032] It should also be understood that the term “comprising” (also referred to as “includes”, “including”, “comprises” and / or “comprising”) as used in this specification specifies the presence of the stated features, integers, steps, operations, elements, and / or components, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof.

[0033] This invention provides a seven-day cycle method for the behavioral domestication of mandarin fish fry in a recirculating aquaculture system. For example... Figure 1The flowchart shown is a seven-day cycle of factory-scale recirculating aquaculture system for the behavioral domestication of mandarin fish fry. The process of this method may include the following steps: S1: On the first day of domestication, mandarin fish fry are placed in a domestication pond with preset environmental conditions in the factory-scale recirculating aquaculture workshop. The environmental conditions are water temperature 26±0.5℃, dissolved oxygen ≥7 mg / L, and pH 7.5-8.5. The mandarin fish fry are healthy, uniformly sized mandarin fish fry with a total length of 3-4 cm. After disinfection, they are released into the domestication pond at a high density of 100 fish / m³. The competitive pressure and social learning effect generated by the dense group are used to accelerate the spread of behavior. In addition, the mandarin fish fry are starved before entering the pond to enhance their initial appetite. The acclimatization ponds are designed as small, modular water units, with each pond having an effective water volume of 510 m³ and a bottom slope of 812°. A central drainage outlet is equipped with an automatic siphon drainage system to ensure that uneaten feed and feces are quickly discharged within 10 minutes after feeding, minimizing the impact of water quality fluctuations on high-frequency feeding. During the pre-release rearing stage, the fry undergo 24-hour starvation. Before release, artificial shelters or slow-flowing water devices are placed below the feeding points in the acclimatization ponds to induce the fry to gather and inhabit near the feeding points. This fully utilizes the competitive pressure and social learning effects of dense groups to enhance the fry's initial appetite, guiding them to stably gather and inhabit the feeding points, thus establishing a solid foundation for acclimatization behavior.

[0034] S2: The goal of the second day of training was to establish a strong conditioned reflex in the fish to feeding signals within a single day. A strategy of "high frequency, strong stimulation, and high feeding rate" was employed. Live bait fish were fed to the mandarin fish fry according to the preset first feeding frequency. Before each feeding, a vertical water flow impact mechanism was activated to induce the fry to establish a stimulus-aggregation conditioned reflex. This mechanism consisted of a high-intensity vertical water flow (creating violent surface disturbance) and specific frequency sound waves (simulating crisis and food signals) released simultaneously in the training pond, forming a complex signal that was difficult to ignore. Subsequently, a large amount of live bait was introduced. Through this "saturation bombardment" training, the fish were forced to closely associate "specific strong stimulus" with "food appearance" within a single day. The first feeding frequency was 4-5 times per day, with feeding times evenly distributed. The daily feeding rate was 3% of the estimated total weight of the mandarin fish fry, aiming to establish positive reinforcement while meeting their high metabolic needs. High-intensity vertical water flow impact is achieved by a high-flow submersible pump or high-pressure air stone installed directly above the feeding point. The diameter of the water splash generated at the impact point must be ≥30 cm. The specific spectrum sound wave includes infrasound components (<100Hz) that simulate the startled escape of the bait fish and the high-frequency impact sound of pellet feed falling on the water surface. Through saturation bombardment training, the fish can establish a strong conditioned reflex in a single day that the composite signal equals the appearance of food, thus activating the fish's high feeding enthusiasm.

[0035] S3: On the third day of acclimatization, stop feeding live bait fish and do not use bait with reduced activity. Instead, use bait that is completely disabled but still alive. Feed the mandarin fish fry with electrocution-disabled live bait according to the preset second feeding frequency to maintain high-intensity conditioned stimulation. The second feeding frequency is 4 times a day, and the feeding rate is reduced to 2.5% of the estimated total weight of the mandarin fish fry. The electrocution-disabled live bait is obtained by processing with a low-voltage pulse electroshock device. The low-voltage pulse electroshock device is set to cause the bait fish to instantly lose 100% of their regular swimming ability, manifested as continuous spasms or rolling over, but with vital signs maintained. The specific parameters are: pulse waveform is a bipolar square wave, field strength 0.8-1.2 V / cm, frequency 8-15. At a frequency of Hz and a duty cycle of 40%, the bait fish passes through the electric field for 0.3-0.6 seconds. While maintaining a strong conditioned stimulus, this disrupts the mandarin fish's inherent feeding perception of smoothly swimming prey, forcing the fry to adapt to atypical live bait and taking a decisive step towards accepting stationary bait. This state of motion is entirely new and unusual for the mandarin fish, but it is highly stimulating. It completely shatters the mandarin fish's inherent expectation of "smoothly swimming" prey, forcing it to adapt to an "atypical live" target, which is a decisive step towards accepting stationary bait.

[0036] S4: On the fourth day of acclimatization, the mandarin fish fry are fed mixed live bait at the preset third feeding frequency. The third feeding frequency is four times a day, with a total feeding rate of 3% of the estimated total weight of the mandarin fish fry. The mixed live bait is a mixture of mechanically damaged live bait (such as fin clipping) and fresh dead bait, with the mixing weight ratio gradually transitioning from 7:3 to 3:7. Mechanically damaged live bait is obtained by causing non-lethal physical damage to the live bait fish, which will produce directionless, struggling movements, further deconstructing the concept of "normal movement". In this step, mixing with dead bait allows the fish to come into contact with both "abnormal movement" and "static" bait in the same feeding. The ratio rapidly transitions from mainly damaged live bait to mainly dead bait within a day, further deconstructing the fry's cognitive dependence on normally moving prey, and using the fry's remaining aggressive impulses to achieve a smooth transition from abnormally moving bait to static bait. The core of this step is to quickly introduce static targets before the fish have formed a fixed pattern of electric shock incapacitating bait, using their remaining aggressive impulses to complete the transition.

[0037] S5: The fifth day of acclimatization is the core step in the transition from "fish-shaped bait" to "non-fish-shaped feed." Begin by scattering pure feed pellets and observing the response. Following the preset fourth feeding frequency (4 times a day, 3.5% feeding rate), feed the mandarin fish fry with embedded bait. The embedded bait is a mixture of artificial feed pellets embedded within the main muscle groups of fresh dead bait fish. The artificial feed pellets have a diameter of 1.0-1.5 mm, and the weight ratio of artificial feed pellets to fresh dead bait fish is 3:7, gradually adjusted to 5:5. The embedding operation is completed using a specially designed multi-needle injection embedding machine. This machine includes a vibrating feeding tray, a multi-needle injection mechanism, and a conveyor belt. After the dead bait fish is positioned, the machine injects a preset number of feed pellets into the dorsal and ventral muscles of the fish at a fixed depth and distance without severely damaging its body shape. When the mandarin fish attacks and eats the dead bait, it inevitably ingests, chews, and swallows the feed pellets along with it. This is a strategy of "forced contact" and "passive acceptance," which allows fish fry to simultaneously ingest formulated feed while swallowing dead bait, thus successfully completing the key transition from fish-shaped bait to non-fish-shaped artificial formulated feed.

[0038] S6: On the sixth day of domestication, a compound feeding mode was adopted, feeding the mandarin fish fry with pure artificial compound feed. Before each feeding, the vertical water flow impact mechanism was activated. The compound feeding mode involved adding a small amount of embedded bait as an inducer, with the proportion of artificial compound feed in the embedded bait being ≥50% to stimulate the fish's aggressive desire. Then, pure artificial compound feed was immediately added. The fish were fed 4 times a day, with a daily feeding rate of 3% of the estimated total weight of the mandarin fish fry. The feeding desire was stimulated again by the water flow stimulation signal.

[0039] S7: On the seventh day of domestication, the mandarin fish fry are fed pure artificial compound feed according to the preset fifth feeding frequency. The fifth feeding frequency is 4 times a day, with a daily feeding rate of 3%. After the cycle ends, individuals that have been successfully domesticated are dynamically selected based on the behavioral indicators of the individual mandarin fish fry. Finally, the standardized behavioral domestication of mandarin fish fry is completed within a short seven-day cycle.

[0040] This invention achieves rapid, efficient, and stable behavioral domestication of mandarin fish fry from their natural feeding on live prey fish to their active feeding on formulated feed through a precisely constructed seven-day tiered domestication process, a customized factory-style recirculating aquaculture environment, multimodal strong-stimulation feeding signals, and a gradient transition system for feed morphology. It is also highly adaptable to the needs of intensive and clean factory-style recirculating aquaculture. This invention completes standardized behavioral domestication of mandarin fish fry within a short seven-day period, possessing significant advantages such as a short domestication cycle, high success rate, strong water quality control, and adaptability to intensive factory-style recirculating aquaculture. It significantly reduces the dependence of mandarin fish farming on live prey fish, effectively improves fry uniformity and farming economics, and provides core technical support for large-scale artificial formulated feed farming of mandarin fish.

[0041] Preferably, the stimulation intensity of the vertical water flow impact mechanism is dynamically adjusted based on the conditioned reflex intensity of the mandarin fish fry population. Specifically, this includes: recording the proportion of mandarin fish fry that gather in the corresponding stimulation area within a preset time period after the vertical water flow impact mechanism is activated as the conditioned reflex intensity index; determining whether the conditioned reflex intensity index of the current stimulation reaches a preset conditioned reflex intensity index threshold; if not, recording the deviation between the threshold and the index as the conditioned reflex intensity index deviation; dynamically matching a preset stimulation intensity adjustment mapping table based on the conditioned reflex intensity index deviation to obtain a stimulation intensity adjustment ratio. The stimulation intensity adjustment mapping table is a pre-stored data table that stores the corresponding association between the conditioned reflex intensity index deviation and the stimulation intensity adjustment ratio; dynamically multiplying the stimulation intensity adjustment ratio by preset water surface velocity adjustment units and sound wave intensity adjustment units to obtain the water surface velocity adjustment ratio and the sound wave intensity adjustment ratio, respectively, based on the water surface velocity adjustment ratio... For example, by dynamically adjusting the surface velocity and sound intensity of the next vertical water flow impact mechanism in conjunction with the sound intensity adjustment ratio, the acclimatization stimulus intensity and the progress of conditioned reflex establishment and group response status of mandarin fish fry are matched and precisely adapted in real time. This avoids the problems of excessively strong stimulus causing stress damage to fry and insufficient stimulus failing to trigger aggregation response, which can be caused by a fixed stimulus intensity. Furthermore, the stimulus parameters can be adaptively optimized according to the response differences of different fry groups and the dynamic changes in the acclimatization process, continuously enhancing the sensitivity and response consistency of the fish group to compound feeding signals, significantly improving the efficiency, stability and accuracy of conditioned reflex establishment, while reducing the negative impact of ineffective and excessive stimuli on fry. This ensures that the acclimatization process is safe, controllable and more standardized, further adapting to the intelligent, refined and efficient acclimatization needs of mandarin fish fry under the factory-style recirculating aquaculture system. It provides core signal regulation support for the stable progress of the entire seven-day acclimatization cycle and the steady improvement of acclimatization effect.

[0042] If the water surface velocity reaches a preset water surface velocity threshold, but the conditioned reflex intensity index still does not reach the conditioned reflex intensity index threshold, then a preset stimulus duration adjustment mapping table is dynamically matched based on the conditioned reflex intensity index deviation to obtain a stimulus duration adjustment ratio. This stimulus duration adjustment ratio is then dynamically multiplied by a preset water flow duration adjustment unit to obtain a water flow duration adjustment ratio. Based on this water flow duration adjustment ratio, the water flow impact duration of the next vertical water flow impact mechanism is dynamically adjusted. The stimulus duration adjustment mapping table is a pre-stored data table that stores the corresponding association between the conditioned reflex intensity index deviation and the stimulus duration adjustment ratio. Similarly, if the sound wave intensity reaches a preset sound wave intensity threshold, but the conditioned reflex intensity index still does not reach the conditioned reflex intensity index threshold, then a preset stimulus duration adjustment mapping table is dynamically matched based on the conditioned reflex intensity index deviation to obtain a stimulus duration adjustment ratio. This stimulus duration adjustment ratio is then dynamically multiplied by a sound wave duration adjustment unit to obtain a sound wave duration adjustment ratio. The ratio, based on the dynamic adjustment of the sound wave duration, regulates the duration of the next vertical water flow impact mechanism. This avoids exceeding safety thresholds by simply increasing flow velocity and sound wave intensity, which could cause stress, panic, or even physical damage to mandarin fish fry. Furthermore, after the stimulation intensity reaches its upper limit, the fine-tuning of the duration dimension continuously strengthens the inducing and stimulating effect of the composite feeding signal on the fish population. This ensures the stable establishment and gradual strengthening of the conditioned reflex of the fish to the feeding signal, effectively solving the limitations of single stimulation intensity adjustment methods, which can easily lead to the failure of domestication signals or inconsistent group responses. It significantly improves the flexibility, safety, and adaptability of domestication stimulation regulation, enabling the entire stimulation regulation system to accurately match the response differences of different fry populations and different domestication stages. This further enhances the intelligence and standardization of the seven-day cycle mandarin fish fry behavior domestication process, providing a more comprehensive and reliable regulatory guarantee for the efficient advancement of the overall domestication process and the stable improvement of the domestication success rate.

[0043] Preferably, the low-voltage pulsed electric shock device includes a dynamic adjustment mechanism for the electrode plate spacing and a dynamic adjustment mechanism for the initial stimulation intensity. The dynamic adjustment mechanism for the electrode plate spacing is achieved by dynamically adjusting the size of the bait fish and the field strength distribution of the device. Specifically, it includes: matching the average body length of the bait fish to a preset initial electrode plate spacing mapping table to obtain the initial electrode plate spacing; the initial electrode plate spacing mapping table is a pre-stored data table that stores the correspondence between the average body length of the bait fish and the recommended electrode plate spacing; comprehensively evaluating the field strength measurement values ​​at each sampling point between the electrode plates to obtain the coefficient of variation of the field strength distribution uniformity; and determining the initial electrode plate spacing, the low-voltage pulsed electric shock device... If the coefficient of variation of the field strength distribution uniformity of the low-voltage pulse electric shock device exceeds a preset threshold for field strength distribution uniformity variation, then the deviation between the threshold and the coefficient of variation is recorded as the uniformity over-deviation. This over-deviation is matched against a preset spacing adjustment mapping table to obtain the electrode plate spacing adjustment ratio. Based on this ratio, the initial electrode plate spacing is dynamically adjusted to obtain the adjusted electrode plate spacing. This adjusted spacing is then used as the electrode plate spacing of the low-voltage pulse electric shock device. The spacing adjustment mapping table stores the corresponding relationship between the uniformity over-deviation and the electrode plate spacing adjustment ratio. Pre-stored data table; otherwise, the initial electrode spacing is used as the electrode spacing of the low-voltage pulse electric shock device. This allows for dual dynamic adaptation of the electrode spacing to the size of the bait fish and the distribution of the electric field strength. It can precisely match the electric field spatial scale for bait fish of different sizes, avoiding the problem of insufficient electric shock for large bait fish and excessive electric shock for small bait fish leading to death or injury due to a fixed electrode spacing. Furthermore, through quantitative evaluation and real-time adjustment of the field strength uniformity, it ensures a stable and uniform electric field strength across the entire area between the electrode plates, eliminating the inconsistent bait treatment effect caused by local field strength anomalies, and strictly ensuring... This method ensures that all bait fish can instantly lose their ability to swim regularly while maintaining vital signs, providing mandarin fish fry with a highly uniform, atypical live bait. This provides stable support for the smooth implementation of the third-day acclimatization step from the source, avoiding interference with the mandarin fish's feeding behavior transformation progress due to fluctuations in bait condition. At the same time, it significantly improves the controllability, consistency, and scalability of electric shock bait treatment, perfectly meeting the needs of batch and standardized preparation of acclimatization bait under the factory-style recirculating aquaculture system. This provides key bait treatment guarantees for the smooth progress of the entire seven-day mandarin fish fry behavior acclimatization process and the overall acclimatization success rate.

[0044] The dynamic adjustment mechanism of initial stimulus intensity is based on the dynamic adjustment of the physiological activity state of the feed fish population after the initial electric shock. Specifically, it includes: dynamically matching the feed fish species with a preset initial electric shock parameter mapping table to obtain initial electric shock intensity parameters, including field strength, electric shock frequency, and duty cycle. The initial electric shock parameter mapping table is a pre-stored data table storing the correlation between different feed fish species and corresponding recommended electric shock intensity parameters; performing the initial electric shock on the feed fish population based on the initial electric shock intensity parameters; if the survival rate of the feed fish is lower than a preset survival rate threshold after the initial electric shock, the deviation between the survival rate threshold and the survival rate is recorded as the survival rate deviation. This survival rate deviation is matched with a preset electric shock intensity adjustment mapping table to obtain the electric shock intensity adjustment ratio. This electric shock intensity adjustment mapping table is a pre-stored data table storing the corresponding correlation between the survival rate deviation and the electric shock intensity adjustment ratio; and dynamically multiplying the electric shock intensity adjustment ratio with preset electric shock intensity adjustment parameters to obtain the adjusted initial electric shock intensity parameters for the next acclimatization cycle. The parameters include the field intensity adjustment unit, the electric shock frequency adjustment unit, and the duty cycle adjustment unit. If the fish population does not fall below the second proportional threshold after the initial electric shock, no additional treatment is required. This achieves precise matching and closed-loop adaptive control between the electric shock stimulation intensity and the characteristics and physiological tolerance of the fish species. This avoids problems such as excessive damage or death of fish due to improper electric shock parameter settings, which would fail to meet the feeding transition needs of mandarin fish fry, or insufficient stimulation that would fail to cause instantaneous disabling of the fish and thus affect the domestication process. It also ensures that the fish processed in batches are in a stable state of disabling but survival, greatly improving the consistency, reliability, and safety of electric shock feed preparation. This ensures that the feed state is always adapted to the key domestication needs of mandarin fish fry transitioning from live feed to static feed, effectively eliminating domestication fluctuations caused by differences in fish species and individual tolerance. This further enhances the standardization and intelligence of the behavior domestication method for mandarin fish fry throughout the seven-day cycle, providing solid and reliable feed processing technology support for the efficient and stable feeding transition of mandarin fish fry under factory recirculating aquaculture systems.

[0045] Preferably, this invention constructs an objective and quantitative screening system that comprehensively covers behavioral response, signal recognition, and feeding efficiency by sequentially setting three progressive judgment criteria: whether the seedlings actively attack the pure artificial feed within a preset first time period (e.g., within 1 minute after the feed enters the water), whether they gather in the corresponding stimulation area within a preset second time period after the vertical water flow impact mechanism is activated (e.g., within 30 seconds after the vertical water flow impact mechanism is activated), and whether the feed intake exceeds a preset feed intake threshold within a preset third time period (e.g., within 30 minutes after the feeding begins). In the preset first time period, it is observed whether the seedlings actively attack the pure artificial feed to directly determine whether they have broken through their nature and completed the transformation of core feeding behavior. In the preset second time period, it is observed whether the seedlings quickly gather in the stimulation area to verify whether they have stably established a conditioned reflex to the feeding signal. In the preset third time period, it is statistically analyzed whether the feed intake of individual seedlings exceeds a preset threshold (e.g., a certain percentage of body weight). Individuals with qualified feeding ability are screened from the perspective of feeding intensity and physiological adaptation. The steps for dynamically screening successfully domesticated individuals based on behavioral indicators of mandarin fish fry include: Step 1: Determine whether the mandarin fish fry attack pure artificial feed within a preset first time period. This directly determines whether the fry have truly broken through their natural instincts and actively consumed non-fish-shaped artificial feed, fundamentally distinguishing whether the core feeding behavior transformation has been completed. If so, proceed to Step 2; otherwise, mark as a domestication failure. Step 2: After activating the vertical water flow impact mechanism, determine whether the mandarin fish fry gather in the impact area corresponding to the vertical water flow impact mechanism within a preset second time period. This verifies whether the fry have stably established a response to the compound feeding signal. The system reflects the behavior of the fish, ensuring efficient and centralized feeding through standardized signals in subsequent factory farming. If the behavior is positive, proceed to step three; otherwise, mark the individual as a failed domestication individual. Step three: Determine whether the feed intake of the individual mandarin fish fry exceeds the preset feed intake threshold within the preset third time period. From the perspective of feeding intensity and physiological adaptation, further screen out weak individuals that only attempt to attack but cannot feed stably or have insufficient feed intake. Strictly ensure that the fry finally marked as successfully domesticated simultaneously possess comprehensive excellent traits such as actively consuming artificial feed, stably responding to feeding signals, and meeting feeding standards. If so, mark the individual as a successfully domesticated individual; otherwise, mark the individual as a failed domestication individual. This screening method completely eliminates the subjectivity and ambiguity of traditional manual observation, enabling quantitative, standardized, and individualized precise judgment of the domestication effect of mandarin fish fry. It can significantly improve the accuracy and consistency of screening successfully domesticated individuals, and quickly separate and eliminate unsuccessful domestication individuals, facilitating refined management by pond division. It effectively ensures the uniformity, feeding stability, and survival rate of the fry population after domestication, providing a reliable fry foundation for the subsequent large-scale and standardized cultivation of mandarin fish fry. It further enhances the practicality, controllability, and industrial aquaculture adaptability of the entire seven-day cycle recirculating aquaculture system for mandarin fish fry behavior domestication.

[0046] Preferably, the present invention further includes a dynamic adjustment mechanism for seedling stocking density, specifically including: counting the proportion of individuals marked as successfully domesticated after the domestication cycle ends, recorded as the domestication success rate; determining whether the domestication success rate is lower than a preset domestication success rate threshold, otherwise no additional processing is performed; if so, determining whether the fish conflict frequency has reached a preset maximum conflict frequency threshold, if so, recording the deviation between the maximum conflict frequency threshold and the fish conflict frequency as the conflict over-bias, matching a preset density adjustment mapping table based on the conflict over-bias to obtain the stocking density adjustment ratio, dynamically adjusting the stocking density adjustment ratio for the next domestication cycle based on the stocking density adjustment ratio to obtain the adjusted stocking density, the density adjustment mapping table being a pre-stored data table storing the conflict over-bias and the corresponding relationship between the stocking density adjustment ratio; if not, gradually increasing the current stocking density according to a preset density adjustment step size to obtain the adjusted stocking density; using the adjusted stocking density as the stocking density for the next domestication cycle, and the adjusted stocking density not exceeding the maximum safe density. By establishing a threshold, a closed-loop dynamic adjustment model is formed, encompassing feedback on domestication effects, monitoring of conflict behaviors, and density-based tiered control. This model effectively preserves the competitive pressure and social learning effects of high-density stocking, accelerating the rapid spread and establishment of domestication behavior in mandarin fish fry. It also effectively avoids problems such as fish aggression, increased stress, and feeding disorders caused by excessively high stocking densities, leading to a decrease in domestication success rates. Simultaneously, it avoids the drawbacks of insufficient group effects, slow behavioral domestication, and low facility utilization efficiency caused by excessively low densities. This achieves dynamic adaptation and optimal balance between stocking density, fish behavior, actual domestication effects, and farm safety. It significantly improves the versatility and stability of the entire domestication method for mandarin fish fry of different sizes and physical conditions, continuously optimizes the controllability and success rate of the seven-day domestication cycle, and further enhances the large-scale, standardized, and intelligent operation of mandarin fish fry domestication under the factory-style recirculating aquaculture system. This provides crucial density control guarantees for the efficient implementation and continuous iteration of the overall domestication program.

[0047] Preferably, the present invention further includes a dynamic adjustment mechanism for feeding control parameters, specifically including: feeding assessment indicators including a feeding attack speed index and an estimated daily feeding rate; feeding control parameters including the proportion of feed types and feeding frequency; obtaining the target feeding assessment index corresponding to each acclimatization day; calculating the deviation feeding assessment index for each acclimatization day based on the deviation between the feeding assessment index for each acclimatization day and the target feeding assessment index for each acclimatization day, the deviation feeding assessment index including the deviation of the feeding attack speed index and the deviation of the estimated daily feeding rate; if the deviation of the feeding attack speed index exceeds a preset attack speed deviation threshold, then the feeding attack speed index is adjusted accordingly. The deviation between the attack speed deviation and the attack speed deviation threshold is recorded as the attack speed over-deviation. This over-deviation is matched against a preset feed adjustment mapping table to obtain the feed adjustment ratio. Based on this ratio, the feed type ratio for the next meal is dynamically adjusted to revert to a feed type more closely resembling the successful feed type from the previous acclimatization stage. This effectively avoids problems such as decreased attack intention and interrupted feeding behavior in fry due to overly rapid feed transition or insufficient feeding stimulation. The feed adjustment mapping table is a pre-stored data table that associates the attack speed over-deviation with the feed adjustment ratio. If the daily feeding rate estimate deviates beyond a preset feeding rate deviation threshold, the feeding rate deviation threshold is then compared with the daily... The deviation of the estimated feeding rate is recorded as the feeding rate over-deviation. This over-deviation is matched against a pre-defined feeding time adjustment mapping table to obtain the feeding time adjustment ratio. Based on this ratio, the feeding interval for the next training day is dynamically adjusted. The feeding time adjustment mapping table is a pre-stored data table that associates the feeding rate over-deviation with the feeding time adjustment ratio, ensuring a high degree of adaptation between the feeding rhythm and the fry's feeding ability and metabolic level. This mechanism achieves a fully intelligent closed-loop process of real-time monitoring of feeding status, quantitative calculation of deviation, and precise control of feeding parameters. It can flexibly adjust the feeding time adjustment based on the actual feeding performance of the mandarin fish fry during the daily training process. Precise adjustment of feed transition progress and feeding strategy can prevent the acclimatization pace from being too fast, which may cause feeding stress and transition failure in fry, while avoiding feed waste, water quality fluctuations and reduced acclimatization efficiency due to delayed regulation. It can significantly improve the stability, adaptability and scientific nature of the feed gradient transition and high-frequency feeding mode within a seven-day cycle, and further enhance the adaptability of the entire acclimatization method to different fry populations and different aquaculture conditions. It can provide a continuous and reliable feeding regulation guarantee for the smooth and efficient transition of mandarin fish fry from live feed to artificial compound feed, and is highly compatible with the standardized, intelligent and efficient mandarin fish fry acclimatization needs under the factory recirculating aquaculture system.

[0048] This invention defines the feeding attack speed index as the average time from when the bait enters the water until the mandarin fish fry launch their first attack. By replacing traditional subjective observation with an objective time value, it completely eliminates errors and ambiguities caused by human assessment, providing reliable core feedback data for the dynamic adjustment of bait type ratios. Simultaneously, it standardizes the estimated daily feeding rate as the ratio of net daily feed intake (the deviation between the daily feed amount and the amount of uneaten feed) to the total weight of the fish population (the product of the average weight of the fish population and the number of surviving fish in the training pond). By integrating multiple key parameters such as feed amount, uneaten feed amount, fish population weight, and number of surviving fish, it can accurately reflect the actual daily feeding intensity, physiological feeding needs, and feed utilization efficiency of the mandarin fish fry, avoiding the bias and distortion caused by estimations based on a single indicator. This provides a reliable core feedback data for the dynamic adjustment of feed type ratios. The adaptive adjustment of parameters such as interval and feeding frequency provides a rigorous data foundation. The clear definition and standardized calculation of the two indicators together construct a quantitative, accurate, reliable, and logically rigorous feeding status assessment system. This allows feeding and density regulation to no longer rely on experience-based judgment, but to implement closed-loop regulation based on measurable, calculable, and comparable objective data. This significantly improves the accuracy, stability, and operability of the dynamic regulation mechanism, effectively avoiding problems such as improper feed transition, disordered feeding rhythm, and unreasonable density configuration caused by ambiguous regulation basis. It continuously ensures a smooth transition of mandarin fish fry feeding behavior within a seven-day cycle, further enhancing the standardization, intelligence, and scientific level of the entire set of factory-scale recirculating aquaculture system mandarin fish fry behavior domestication methods, and providing key indicator support and data guarantee for the stable improvement of domestication efficiency and success rate.

[0049] like Figure 2The diagram shown is a structural schematic of a seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry provided in an embodiment of the present invention. The present invention also provides a seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry, used to implement a seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry. Specifically, it includes an environmental control module, a feeding stimulation module, and an enhanced intelligent monitoring and real-time decision-making module. The environmental control module is used to control the environment of the recirculating tank at preset environmental conditions and is equipped with a local rapid heating device, which can briefly raise the local water temperature at the feeding point by 12°C before feeding to stimulate metabolism and appetite. The feeding stimulation module includes a precise control unit for the feed status and a multimodal stimulation delivery unit. The precise control unit for the feed status is used to integrate low-voltage pulse electricity... The system includes an electric shock device, a mechanical damage device, and a feed embedding device, which can prepare the required feed in batches according to the daily plan. The multimodal stimulus delivery unit provides focused light spot stimulation at the beginning of feeding, including a device that generates high-intensity vertical water flow, a wide-spectrum underwater sonar array, and an underwater focused LED light with adjustable color temperature and flash frequency. The enhanced intelligent monitoring and real-time decision-making module includes a high-frequency data acquisition system (water quality sensor, high-speed camera), an edge computing server, and real-time control software, which is used to calculate the feeding attack speed index, the estimated daily feeding rate, and the conditioned reflex intensity index in real time. When there is a deviation from the daily target, it immediately generates an adjustment plan, such as "increase the electric shock intensity of the next meal by 5%" or "change the third feeding to pure dead bait".

[0050] The above description is only an optional embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A seven-day cycle factory-scale recirculating aquaculture system for the behavioral domestication of mandarin fish fry, characterized in that, The method includes: S1: On the first day of domestication, mandarin fish fry are introduced into domestication ponds with preset environmental conditions in the factory-style recirculating water domestication workshop. S2: On the second day of domestication, live bait fish are fed to the mandarin fish fry according to the preset first feeding frequency. Before each feeding, the vertical water flow impact mechanism is activated to induce the mandarin fish fry to establish a stimulus aggregation conditioned reflex. The vertical water flow impact mechanism is a high-intensity vertical water flow and a specific spectrum sound wave released synchronously in the domestication pool. The stimulation intensity of the vertical water flow impact mechanism is dynamically adjusted based on the conditioned reflex intensity of the mandarin fish fry population, specifically including: After the vertical water flow impact mechanism is activated, the proportion of mandarin fish fry that gather in the corresponding stimulation area within a preset time period is recorded as the conditioned reflex intensity index. Determine whether the conditioned reflex intensity index of the current stimulus reaches the preset conditioned reflex intensity index threshold. If it does not reach the threshold, the deviation between the conditioned reflex intensity index threshold and the conditioned reflex intensity index is recorded as the conditioned reflex intensity index deviation. Based on the conditioned reflex intensity index deviation, dynamically match the preset stimulus intensity adjustment mapping table to obtain the stimulus intensity adjustment ratio. The stimulus intensity adjustment mapping table is a pre-stored data table that stores the corresponding association between the conditioned reflex intensity index deviation and the stimulus intensity adjustment ratio. The stimulation intensity adjustment ratio is dynamically processed for the preset water surface velocity adjustment unit and sound wave intensity adjustment unit to obtain the water surface velocity adjustment ratio and sound wave intensity adjustment ratio. The water surface velocity and sound wave intensity of the next vertical water flow impact mechanism are dynamically adjusted based on the water surface velocity adjustment ratio and sound wave intensity adjustment ratio. If the water surface velocity reaches the preset water surface velocity threshold, but the conditioned reflex intensity index still does not reach the conditioned reflex intensity index threshold, then the preset stimulus duration adjustment mapping table is dynamically matched based on the conditioned reflex intensity index deviation to obtain the stimulus duration adjustment ratio. The stimulus duration adjustment ratio is then dynamically processed against the preset water flow duration adjustment unit to obtain the water flow duration adjustment ratio. Based on the water flow duration adjustment ratio, the water flow impact duration of the next vertical water flow impact mechanism is dynamically adjusted. The stimulus duration adjustment mapping table is a pre-stored data table that stores the conditioned reflex intensity index deviation and the corresponding correlation of the stimulus duration adjustment ratio. If the sound wave intensity reaches the preset sound wave intensity threshold, but the conditioned reflex intensity index does not reach the conditioned reflex intensity index threshold, then the preset stimulus duration adjustment mapping table is dynamically matched based on the conditioned reflex intensity index deviation to obtain the stimulus duration adjustment ratio. The stimulus duration adjustment ratio is then dynamically processed to obtain the sound wave duration adjustment unit to obtain the sound wave duration adjustment ratio. Based on the sound wave duration adjustment ratio, the sound wave transmission duration of the next vertical water flow impact mechanism is dynamically adjusted. S3: On the third day of domestication, the mandarin fish fry were fed with live bait that had been electrocuted according to the preset second feeding frequency in order to maintain high intensity conditioned stimulation. The live bait that had been electrocuted was obtained by processing with a low-voltage pulse electroshock device. S4: On the fourth day of domestication, the mandarin fish fry are fed mixed live bait at the preset third feeding frequency. The mixed live bait is a mixture of mechanically damaged live bait and fresh dead bait. The mechanically damaged live bait is obtained by causing non-lethal physical damage to the live bait fish. S5: On the fifth day of domestication, the mandarin fish fry are fed embedded bait at the preset fourth feeding frequency. The embedded bait is a mixture of artificial compound feed pellets embedded in the main muscle groups of fresh dead bait fish. S6: On the sixth day of domestication, a compound feeding mode is adopted, and pure artificial compound feed is fed to the mandarin fish fry. The vertical water flow impact mechanism is activated before each feeding. S7: On the seventh day of domestication, the mandarin fish fry were fed pure artificial compound feed at the preset fifth feeding frequency, and successfully domesticated individuals were dynamically screened based on the individual behavioral indicators of the mandarin fish fry after the cycle ended. The steps for dynamically screening successfully domesticated individuals based on behavioral indicators of individual mandarin fish fry include: Step 1: Determine whether the individual mandarin fish fry attacks the pure artificial feed within the preset first time. If yes, proceed to Step 2; otherwise, mark it as an individual that has failed to be domesticated. Step 2: After activating the vertical water flow impact mechanism, determine whether the individual mandarin fish fry gather in the impact area corresponding to the vertical water flow impact mechanism within the preset second time. If so, proceed to Step 3; otherwise, mark them as individuals that have failed to be domesticated. Step 3: Determine whether the feeding amount of individual mandarin fish fry exceeds the preset feeding threshold within the preset third time period. If so, mark them as successfully domesticated individuals; otherwise, mark them as unsuccessfully domesticated individuals.

2. The seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry as described in claim 1, characterized in that, The low-voltage pulse electric shock device includes a dynamic adjustment mechanism for the electrode plate spacing and a dynamic adjustment mechanism for the initial stimulation intensity. The dynamic adjustment mechanism for the electrode plate spacing is based on the body size of the bait fish and the field strength distribution of the device, specifically including: The average body length of the bait fish is matched with a preset initial electrode plate spacing mapping table to obtain the initial electrode plate spacing. The initial electrode plate spacing mapping table is a pre-stored data table that stores the correspondence between the average body length of the bait fish and the recommended electrode plate spacing. The coefficient of variation of the uniformity of the field strength distribution is obtained by comprehensively evaluating the field strength measurements at each sampling point between the electrode plates. Determine whether the coefficient of variation of the uniformity of the electric field distribution of the low-voltage pulse electric shock device exceeds the preset threshold for uniformity of the electric field distribution under the initial electrode plate spacing. If so, the deviation between the threshold for uniformity of the electric field distribution and the coefficient of variation of the uniformity of the electric field distribution is recorded as the uniformity over-bias. The uniformity over-bias is matched with a preset spacing adjustment mapping table to obtain the electrode plate spacing adjustment ratio. The initial electrode plate spacing is dynamically adjusted based on the electrode plate spacing adjustment ratio to obtain the adjusted electrode plate spacing. The adjusted electrode plate spacing is used as the electrode plate spacing of the low-voltage pulse electric shock device. The spacing adjustment mapping table is a pre-stored data table that stores the corresponding association between the uniformity over-bias and the electrode plate spacing adjustment ratio. Otherwise, the initial electrode plate spacing will be used as the electrode plate spacing of the low-voltage pulse electric shock device.

3. The seven-day cycle factory-scale recirculating aquaculture system for domesticating mandarin fish fry behavior as described in claim 2, characterized in that, The initial stimulus intensity dynamic adjustment mechanism is based on the dynamic adjustment of the physiological activity state of the feed fish population after the initial power-on, specifically including: Based on the dynamic matching of bait fish species and the preset initial electric shock parameter mapping table, the initial electric shock intensity parameters are obtained. The electric shock intensity parameters include field strength, electric shock frequency and duty cycle. The initial electric shock parameter mapping table is a pre-stored data table that stores the correlation between different bait fish species and the corresponding recommended electric shock intensity parameters. The feed fish are initially energized based on the initial electric shock intensity parameters. If the survival rate of the feed fish is lower than the preset survival rate threshold after the initial energization, the deviation between the survival rate threshold and the survival rate of the feed fish is recorded as the survival rate deviation. The survival rate deviation is matched with a preset electric shock intensity adjustment mapping table to obtain the electric shock intensity adjustment ratio. The electric shock intensity adjustment mapping table is a pre-stored data table that stores the corresponding relationship between the survival rate deviation and the electric shock intensity adjustment ratio. The electric shock intensity adjustment ratio is dynamically processed according to the preset electric shock intensity adjustment parameters to obtain the initial electric shock intensity parameters after adjustment. The electric shock intensity adjustment parameters include field intensity adjustment units, electric shock frequency adjustment units, and duty cycle adjustment units. If the number of bait fish does not fall below the second threshold after the first power-on, no additional treatment is required.

4. The seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry as described in claim 1, characterized in that, It also includes a dynamic adjustment mechanism for seedling stocking density, specifically including: After the domestication cycle ends, the proportion of individuals marked as successfully domesticated is recorded as the domestication success rate; it is then determined whether the domestication success rate is lower than the preset domestication success rate threshold, otherwise no additional processing is performed; If so, determine whether the fish conflict frequency has reached the preset maximum conflict frequency threshold. If it has, record the deviation between the maximum conflict frequency threshold and the fish conflict frequency as the conflict over-bias. Based on the conflict over-bias, match the preset density adjustment mapping table to obtain the release density adjustment ratio. Based on the release density adjustment ratio, dynamically adjust the release density adjustment ratio for the next domestication cycle to obtain the adjusted release density. The density adjustment mapping table is a pre-stored data table that stores the conflict over-bias and the release density adjustment ratio. If the target is not reached, the current density will be gradually increased according to the preset density adjustment step size to obtain the adjusted density. The adjusted stocking density will be used as the stocking density for the next acclimatization cycle, and the adjusted stocking density will not exceed the maximum safe density threshold.

5. The seven-day cycle recirculating aquaculture system for the behavioral domestication of mandarin fish fry as described in claim 1, characterized in that, It also includes a dynamic adjustment mechanism for feeding control parameters, specifically including: Feeding assessment indicators include the feeding attack speed index and the estimated daily feeding rate; the feeding control parameters include the proportion of feed types and the feeding frequency. Obtain the target feeding assessment indicators for each domestication day; Based on the deviation between the feeding assessment index of each domestication day and the target feeding assessment index of each domestication day, the deviation feeding assessment index of each domestication day is calculated. The deviation feeding assessment index includes the deviation of the feeding attack speed index and the deviation of the daily feeding rate estimate. If the feeding attack speed index deviation exceeds the preset attack speed deviation threshold, the deviation between the feeding attack speed index deviation and the attack speed deviation threshold is recorded as the attack speed over-deviation. The attack speed over-deviation is matched with a preset bait adjustment mapping table to obtain the bait adjustment ratio. Based on the bait adjustment ratio, the bait type ratio of the next meal is dynamically adjusted. The bait adjustment mapping table is a pre-stored data table that stores the corresponding association between the attack speed over-deviation and the bait adjustment ratio. If the deviation of the estimated daily feed intake rate exceeds the preset feed intake rate deviation threshold, the deviation between the feed intake rate deviation threshold and the estimated daily feed intake rate is recorded as the feed intake rate over-deviation. The feed intake rate over-deviation is matched with a preset feeding time adjustment mapping table to obtain the feeding time adjustment ratio. Based on the feeding time adjustment ratio, the feeding time interval of the next acclimatization day is dynamically adjusted. The feeding time adjustment mapping table is a pre-stored data table that stores the corresponding association between the feed intake rate over-deviation and the feeding time adjustment ratio.

6. The seven-day cycle recirculating aquaculture system for training mandarin fish fry behavior as described in claim 5, characterized in that, The feeding attack speed index is the average time from when the bait enters the water until it is first attacked by each mandarin fish fry. The estimated daily feeding rate is a comprehensive assessment of the daily feed intake, the daily uneaten feed amount, and the average weight of the fish population. The estimated daily feeding rate is obtained as follows: The difference between the amount of feed given on the day and the amount of uneaten feed on the day is recorded as the net feed intake. The total weight of the group is the product of the average weight of the fish and the number of surviving mandarin fish in the acclimatization pond. The ratio of net food intake to total population weight is the estimated daily food intake rate.

7. A seven-day cycle factory-scale recirculating aquaculture system for the behavioral domestication of mandarin fish fry, used to implement the seven-day cycle factory-scale recirculating aquaculture system for the behavioral domestication of mandarin fish fry as described in any one of claims 1-6, characterized in that, The system includes an environmental control module, a feeding and stimulation module, and an enhanced intelligent monitoring and real-time decision-making module. The environmental control module is used to control the environment of the circulating pool under preset environmental conditions and is equipped with a local rapid heating device. The feeding stimulation module includes a feed state precision control unit and a multimodal stimulation delivery unit. The feed state precision control unit is used to integrate a low-voltage pulse electric shock device, a mechanical damage device, and a feed embedding device, and can prepare the required feed state in batches according to the daily plan. The multimodal stimulation delivery unit is used to provide focused light spot stimulation in the early stage of feeding. The enhanced intelligent monitoring and real-time decision-making module includes a high-frequency data acquisition system, an edge computing server, and real-time control software. It is used to calculate the feeding attack speed index, the estimated daily feeding rate, and the conditioned reflex intensity index in real time, and to generate an adjustment plan when there is a deviation from the daily target.

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