Application and method for preventing and treating lysimachia christinae by using isotropic agent
By using trans-9-tetradecene-2,2-difluoroacetate to prepare mating silk, the mating of the longhorn beetle *Eriocheir sinensis* was interfered with, thus solving the environmental threats and pest resistance problems associated with chemical pesticide control and achieving environmentally friendly and highly efficient control.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HEBEI YIHANG NEW ENERGY TECH
- Filing Date
- 2026-01-16
- Publication Date
- 2026-04-24
AI Technical Summary
Existing technologies lack effective means to control the longhorn beetle, the use of chemical pesticides leads to pesticide resistance and environmental threats, and there is a lack of environmentally friendly control methods.
Trans-9-tetradecene-2,2-difluoroacetate was used as a mating agent to prepare mating silk, which was then suspended in the field to interfere with the mating of male and female longhorn beetles. Polyvinyl chloride capillary slow-release carriers were used to avoid harm to the environment and natural enemies.
It significantly reduces the mating rate of the longhorn beetle, has a long-lasting effect, is easy to operate, is safe for the environment and natural enemies, and is suitable for large-scale control to reduce insect population density.
Smart Images
Figure CN121909986A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of agricultural technology, specifically to an application and method for controlling the longhorn beetle (Gnaphalium affineum) using a maze agent. Background Technology
[0002] The coffee longhorn beetle (Xylotrechus quadrips), also known as the coffee longhorn beetle, belongs to the order Coleoptera and the family Cermbycidae. It is a major stem-boring pest that damages the economic crop coffee. Coffee is a perennial evergreen shrub or small tree belonging to the family Rubiaceae, with a global cultivation area of approximately 11 million hectares. As the world's second-largest traded commodity and one of the most valuable exports from developing countries, coffee production involves more than 25 million people and has extremely high economic value. The coffee longhorn beetle's larvae build tunnels in the trunk or thicker branches of coffee trees, obstructing nutrient transport to the plant. This can lead to poor plant growth or, in severe cases, breakage or death of the trunk or branches at the site of damage, resulting in significant economic losses. Statistics show that over 9 million coffee plants in India have been damaged by the longhorn beetle, resulting in economic losses of approximately US$400 million. The damage continues to cause economic losses of US$175 million to US$260 million annually. This insect first infested Arabica coffee in India and is now widely distributed in coffee-growing countries and regions such as Sri Lanka, Myanmar, Vietnam, Laos, Thailand, and China. Yunnan Province is my country's main Arabica coffee (Coffea arabica) producing area, ranking first in the country in both planting area and yield, greatly promoting local rural economic development and increasing farmers' income. The longhorn beetle has occurred in Baoshan, Dehong, Dali, Wenshan, and Pu'er prefectures and cities in Yunnan Province, with a damage rate as high as 86.23%. To control the damage caused by the longhorn beetle to the coffee industry, many researchers have conducted studies on its morphological characteristics, biological features, and control methods, but to date, effective means to curb its damage are still lacking. Traditionally, chemical pesticides are the primary control method. However, the extensive use of chemical pesticides not only leads to pesticide resistance in pests, but also poses a threat to human and environmental safety.
[0003] Using sex pheromones to control the longhorn beetle (Gnatifida spp.) is one of the effective measures. Insect sex pheromones come directly from the insect body and do not harm humans or the environment. They help slow down the development of insect resistance to chemical pesticides, protect natural enemy species and populations, ensure environmental and agricultural product safety, and promote sustainable agricultural development. Insect pheromones, as a "third-generation pesticide," have been successfully used in pest control through methods such as mating interference and mass trapping. Currently, using the mating interference method to disrupt the mating between male and female moths has become a hot topic in pest control. The principle of mating interference is to use mating silk to slowly release large amounts of pheromones or similar components to mask the location of female adults, misleading male adults into finding them and delaying or preventing mating. The significant reduction in effective eggs directly leads to a decrease in insect population density, thereby achieving the purpose of control. Summary of the Invention
[0004] To address the aforementioned problems, the present invention aims to provide an application and method for controlling the longhorn beetle using a mating agent. Using trans-9-tetradecene-2,2-difluoroacetate as a mating agent can significantly interfere with mating between male and female longhorn beetles, and is safe for the environment and natural enemies. It can be used for large-scale control of this pest and is of great significance for the integrated management of the longhorn beetle.
[0005] First, the present invention provides an application of using a decoy agent to control the longhorn beetle, wherein the decoy agent is trans-9-tetradecene-2,2-difluoroacetate.
[0006] Secondly, this invention provides a method for controlling the longhorn beetle *Ceratophyllum demersum* using a maze agent. The maze agent, trans-9-tetradecene-2,2-difluoroacetate, is processed into maze filaments and placed in farmland infested by *Ceratophyllum demersum*. The specific preparation steps of the maze filaments are as follows: the maze agent, trans-9-tetradecene-2,2-difluoroacetate, is dissolved in chlorinated paraffin and then added into a polyvinyl chloride capillary tube. Both ends are sealed to obtain a maze agent release carrier, which is the maze filament.
[0007] Optionally, the dosage of trans-9-tetradecene-2,2-difluoroacetate is 100-200 mg / root, with 30-60 mazes per acre.
[0008] Preferably, the dosage of trans-9-tetradecene-2,2-difluoroacetate is 100-150 mg / wire, and 40-60 mazes are set per acre.
[0009] Optimally, the dosage of trans-9-tetradecene-2,2-difluoroacetate is 150 mg / wire, with 40 mazes per acre.
[0010] Compared with the prior art, the beneficial effects of the present invention are as follows: 1) Trans-9-tetradecene-2,2-difluoroacetate is slowly released into the field through a polyvinyl chloride capillary. When the amount of trans-9-tetradecene-2,2-difluoroacetate dispersed in the field air reaches a certain level, it can interfere with and block the intraspecific chemical communication of the longhorn beetle, thereby reducing its mating rate.
[0011] 2) Field tests show that the mating agent of this invention has a significant slow-release effect, with a field residual effect of more than 3 months. Furthermore, the effective component of the mating agent is greater than 100 mg, and with more than 30 mating threads per acre, the mating rate is higher than 80%.
[0012] 3) The maze agent of the present invention can significantly interfere with the mating between male and female longhorn beetles, has a long-lasting effect, is safe for the environment and natural enemies, is non-toxic and harmless to humans, is simple to operate, and can be used for large-scale control of this pest, which is of great significance for the integrated management of longhorn beetles. Attached Figure Description
[0013] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.
[0014] Figure 1 Structural diagram of trans-9-tetradecene-2,2-difluoroacetate. Detailed Implementation
[0015] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0016] Example 1: Preparation of Misalignment Wires Polyvinyl chloride capillary tubes (20 cm in length, 1.5 mm in inner diameter, and 0.5 mm in wall thickness) were selected as the sustained-release carrier, and chlorinated paraffin (CP52) was used as the solvent. 100 mg, 150 mg, and 200 mg of trans-9-tetradecene-2,2-difluoroacetate were dissolved in chlorinated paraffin, mixed thoroughly to prepare a mother liquor of a certain concentration, and a certain volume of the mother liquor was dispensed into the capillary tube using a pipette. The two ends of the capillary tube were then heat-sealed to prepare a sustained-release device for the maze agent of the longhorn beetle, which is the maze wire.
[0017] Example 2 Field test of apheresis agent on *Eupolyphaga sinensis* and *Eupolyphaga chinensis* The experimental field, covering approximately 200 mu (about 13.3 hectares), was selected from areas severely affected by the Chinese longhorn beetle. Divided into five plots, each containing a control and a mating zone, with a minimum distance of 1200 m between plots. The effective components of the mating agent in the mating silk were 100 mg, 150 mg, and 200 mg, and the silk was suspended at a height of approximately 150 cm. To further illustrate the mating effect, the attractant component of the Chinese longhorn beetle, (S)-2-hydroxy-3-decanoate (J Chem Ecol 32: 195-219. 2006), was used as a control, with dosages of 100 mg, 150 mg, and 200 mg. See the table below for details.
[0018] The inhibition rate of mating of the longhorn beetle by different doses of mating agent and the number of mating threads placed.
[0019]
[0020] The results in the table show that, compared with the sex pheromone control, trans-9-tetradecene-2,2-difluoroacetate has a significant disorientation effect on the longhorn beetle *Eriocaulon buergerianum*, and its disorientation effect is superior to that of the sex pheromone control at the same dose.
[0021] At the same dosage, the mating effect becomes more pronounced with increasing planting density. When the dosage is greater than 150 mg and more than 30 plants are planted per acre, the mating rate is greater than 80%.
[0022] In summary, this invention provides a mating agent for interfering with the mating of the longhorn beetle *Echinochloa crus-galli*. Through examples, it was found that this mating agent can interfere with the mating communication between male and female *Echinochloa crus-galli*. When the dosage of trans-9-tetradecene-2,2-difluoroacetate is 150 mg and 40 mating threads are placed per acre, the mating rate is greater than 90%, and the mating rate is superior to that of mating threads with the same dosage of attractant components. The mating agent for *Echinochloa crus-galli* of this invention is environmentally and pest-safe, simple to operate, and can be used for large-scale control of this pest, which is of great significance for the integrated management of *Echinochloa crus-galli*.
[0023] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.
Claims
1. An application of a decoy agent for controlling the longhorn beetle, characterized in that, The demagnetizing agent is trans-9-tetradecene-2,2-difluoroacetate.
2. A method for controlling the longhorn beetle *Eriocheir sinensis* using a decoy agent, characterized in that, The maze agent trans-9-tetradecene-2,2-difluoroacetate was processed into maze filaments and placed in farmland infested by the Chinese longhorn beetle. The specific preparation steps of the maze filaments are as follows: the maze agent trans-9-tetradecene-2,2-difluoroacetate was dissolved in chlorinated paraffin and then added into a polyvinyl chloride capillary tube. Both ends were sealed to obtain the maze agent release carrier, which is the maze filament.
3. The method according to claim 2, characterized in that, The dosage of trans-9-tetradecene-2,2-difluoroacetate is 100-200 mg / root, and 30-60 mazes are set per acre.
4. The method according to claim 2, characterized in that, The dosage of trans-9-tetradecene-2,2-difluoroacetate is 100-150 mg / root, with 40-60 mazes per acre.
5. The method according to claim 2, characterized in that, The dosage of trans-9-tetradecene-2,2-difluoroacetate is 150 mg / wire, with 40 mazes per acre.