Pipeline type visible component detection device with micro-flow metering pump and method
By introducing a microfluidic metering pump and buffer system into the formed element detection device, the problem of difficult quantitative analysis in the prior art is solved, and accurate measurement and efficient detection of microscopic samples are achieved, while reducing system complexity and cost.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHENZHEN ANLV MEDICAL TECH CO LTD
- Filing Date
- 2024-10-22
- Publication Date
- 2026-04-24
AI Technical Summary
Existing formed element detection devices lack pipelines and microfluidic metering pumps, making it difficult to perform quantitative automated analysis of large numbers of microscopic samples, and the systems are complex and costly.
The device employs a pipeline-type formed element detection system with a microfluidic metering pump, comprising a detection module, a drain pipe, an inlet pipe, a sample inlet head, a camera assembly, and a microfluidic metering pump. The microfluidic metering pump measures the microscopic sample, and AI is used to identify the microscopic images for quantitative analysis. The sample flow is driven by a peristaltic pump and a piezoelectric pump, and a buffer tank and pressure balancing system are provided to ensure precise control.
It enables quantitative analysis of microscopic samples, reduces system complexity and cost, improves detection efficiency and result accuracy, and simplifies the detection process.
Smart Images
Figure CN121917544A_ABST
Abstract
Description
Technical Field
[0001] This application belongs to the field of medical device technology, and in particular relates to a pipeline-type formed element detection device. Background Technology
[0002] Medical testing devices for formed elements are high-precision, highly complex medical instruments that include sophisticated microscopic imaging systems and image analysis and processing systems. They are a comprehensive integration of disciplines such as biotechnology, medical technology, high-precision instruments, computer science, and artificial intelligence graphics analysis technology. The system is complex and requires a large number of sensors, control and detection devices. Determining the functions of each component of the system and how to reduce the complexity of research and development, equipment costs, and maintenance costs are huge technical challenges.
[0003] Existing formed element detection devices typically do not include piping or microfluidic pumps; they simply magnify a small amount of microscopic sample within the chip cavity to obtain magnified images for formed element analysis. How to perform automated quantitative formed element analysis on larger volumes of microscopic samples remains a technical problem to be solved. Summary of the Invention
[0004] This application proposes that the components performing different functions within a complex formed element detection device be rationally modularized and standardized, which can form a standardized industrial chain. Different manufacturers can research different modules, which can significantly reduce the difficulty of research and development and reduce equipment costs.
[0005] The solution to the above-mentioned technical problems is a pipeline-type formed element detection device with a microfluidic metering pump, including a detection module, a drain pipe, an inlet pipe, a sample inlet, a camera assembly, and a microfluidic metering pump. The detection module includes an inlet, a detection chamber, and a drain outlet. The detection chamber is connected to the inlet and the drain outlet. The inlet pipe is connected to the inlet. The drain pipe is connected to the drain outlet. The sample inlet is connected to the inlet pipe. The camera assembly is used to capture images of the microscopic sample inside the detection chamber. The microfluidic metering pump is connected in series in the inlet or the drain pipe. The microfluidic metering pump is used to measure the volume VA of the microscopic sample flowing through the detection chamber. The camera assembly is used to capture a microscopic image of the microscopic sample with a volume equal to VA.
[0006] The aforementioned inline formed element detection device with microfluidic metering pump also includes an inlet pump and a buffer tank; the sample inlet head, inlet pump, and buffer tank are connected in series in the inlet pipeline, and the inlet pump is used to drive external microscopic samples, air, or cleaning liquid into the buffer tank.
[0007] The upper part of the aforementioned buffer box includes a pressure balancing opening, which is connected to a pressure balancing valve.
[0008] The above-mentioned pipeline-type formed element detection device with microfluidic metering pump includes any one of the following technical features: TA1: the above-mentioned inlet pump is a peristaltic pump; TA2: the above-mentioned microfluidic metering pump is a piezoelectric pump.
[0009] The aforementioned pipeline-type formed element detection device with microfluidic metering pump also includes a drain hose and a waste liquid collection device. One end of the drain hose is connected to the drain pipeline, and the other end is connected to the waste liquid collection device.
[0010] The aforementioned pipeline-type formed element detection device with microfluidic metering pump also includes an illumination component, which is located above or below the detection module, respectively.
[0011] The solution to the above-mentioned technical problem in this application can also be a pipeline-type formed element detection method, including the following steps: A30: a microfluidic metering pump drives a microscopic sample with a volume equal to VA to flow through the detection chamber; A40: a microscopic sample with a volume equal to VA is photographed to obtain a microscopic image; A50: AI identifies the formed element categories in the microscopic image and obtains the quantity of the selected formed elements; A60: the content of formed elements per unit volume is obtained.
[0012] The above-mentioned pipeline-type formed element detection method further includes, A10: pipeline cleaning step; step A10 further includes any one of the following technical features: TB1: further includes step A11: pumping cleaning fluid into the detection chamber; TB2: further includes step A12: pumping air into the detection chamber.
[0013] The above-mentioned pipeline-type formed element detection method also includes A20: the sample injection step.
[0014] In step A20 above, the microscopic sample is pumped into the buffer tank using a peristaltic pump; or in step A30, the microscopic sample is pumped from the buffer tank into the detection chamber using a microfluidic metering pump.
[0015] The technical effect of the above solution is that the microfluidic metering pump can measure the microscopic sample flowing through the detection chamber and obtain the volume VA of the microscopic sample flowing through the detection chamber. Therefore, the volume of the microscopic sample corresponding to the magnified microscopic image can be obtained, and thus accurate quantitative analysis of formed elements can be performed based on the microscopic image of the microscopic sample.
[0016] The technical advantages of the above solution are as follows: the sample inlet head, the liquid inlet pump, and the buffer tank are connected in series in the liquid inlet pipeline. The liquid inlet pump drives the external microscopic sample, air, or cleaning liquid into the buffer tank, which provides buffer space for the microscopic sample, allowing for more accurate microfluidic measurement of the sample entering the detection module. For some samples, partial precipitation and stratification measurement of formed elements can also be performed.
[0017] The technical effect of the above solution is that the microscopic sample is pumped into the buffer box by a peristaltic pump. The upper part of the buffer box includes a pressure balance opening, which is connected to the air pressure balance valve, so that the buffering can be carried out smoothly.
[0018] The technical effect of the above solution is that the microfluidic metering pump is a piezoelectric pump, which is suitable for driving micro-sized microscopic samples and can accurately control the amount of microscopic samples entering the detection cavity.
[0019] The technical effect of the above solution is that the lighting component and the camera component are located above or below the detection module, respectively, with flexible relative positions, making it easier to obtain clear photos.
[0020] The technical advantages of the above solution are as follows: a microfluidic pump drives a microscopic sample with a volume equal to VA to flow through the detection chamber; a microscopic image of the sample equal to VA is obtained; AI identifies the formed elements in the microscopic image and obtains the quantity of the selected formed elements; the content of formed elements per unit volume is obtained. The entire process is simple, efficient, and quantitatively accurate.
[0021] The technical effect of the above solution is that the microscopic sample is pumped from the buffer tank into the detection chamber by a microfluidic metering pump, which can accurately control the flow rate.
[0022] The technical effects of the above solution are: the cleaning pipeline facilitates the reuse of the testing chamber, improves testing efficiency, and reduces testing costs.
[0023] The technical effect of the above solution is that the cleaning liquid and gas can be used for cleaning, either individually or sequentially, to ensure the cleaning effect, the accuracy of each analysis result, and prevent contamination. Attached Figure Description
[0024] Figures 1 to 4 This is a schematic diagram of a pipeline-type formed element detection device. Figures 1 to 4 ;
[0025] Figure 5 This is a schematic diagram of the detection module in a pipeline-type formed element detection device;
[0026] Figures 6 to 10 This is a schematic diagram of a pipeline-type formed element detection method. Figures 1 to 5 . Detailed Implementation
[0027] The contents of this application will be further described in detail below with reference to the accompanying drawings.
[0028] It should be noted that the following description of preferred embodiments of this application does not constitute any limitation on this application. The description of preferred embodiments is merely an illustration of the general principles of this application. The embodiments described in this application are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0029] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the equipment or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this application. Furthermore, the terms "first," "second," and technical features numbered with Arabic numerals 1, 2, 3, etc., and designations such as "A" and "B," are used for descriptive purposes only, for ease of explanation, and do not represent a temporal or spatial order; they should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first," "second," and numbered with Arabic numerals 1, 2, 3, etc., may explicitly or implicitly include one or more of that feature. In the description of this application, "several" means two or more, unless otherwise expressly and specifically defined.
[0030] like Figure 1 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, the device includes a detection module, a drain pipe, an inlet pipe, a sample inlet head, a camera assembly, and a microfluidic metering pump. Figure 5 The detection module includes an inlet, a detection chamber, and an outlet; the detection chamber is connected to the inlet and outlet. An inlet pipe is connected to the inlet; an outlet pipe is connected to the outlet; a sample inlet head is connected to the inlet pipe; a camera assembly is used to capture images of the microscopic sample inside the detection chamber; a microfluidic metering pump is connected in series in the inlet or outlet pipe; the microfluidic metering pump is used to measure the volume VA of the microscopic sample flowing through the detection chamber; the camera assembly captures a microscopic image of the microscopic sample with a volume equal to VA.
[0031] like Figure 2 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, an inlet pump and a buffer tank are also included; the sample inlet head, the inlet pump, and the buffer tank are connected in series in the inlet pipeline, and the inlet pump is used to drive external microscopic samples, air, or cleaning liquid into the buffer tank.
[0032] like Figure 3 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, the upper part of the buffer tank includes a pressure balancing opening, which is connected to a pressure balancing valve.
[0033] like Figure 3 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, the inlet pump is a peristaltic pump.
[0034] like Figures 1 to 3 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, the microfluidic metering pump is a piezoelectric pump. A piezoelectric pump is a novel type of fluid actuator. It does not require an additional drive motor; instead, it utilizes the inverse piezoelectric effect of piezoelectric ceramics to deform a piezoelectric oscillator, which then generates a change in the volume of the pump chamber to achieve fluid output. Alternatively, it uses the piezoelectric oscillator to generate ripples to transport liquid. Piezoelectric pumps possess characteristics not found in traditional pumps.
[0035] like Figures 2 to 4 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, a drain hose and a waste liquid collection device are also included. One end of the drain hose is connected to the drain pipeline, and the other end is connected to the waste liquid collection device.
[0036] like Figures 2 to 4 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, a Z-axis slide assembly is further included. The Z-axis slide assembly is connected to a camera assembly. The Z-axis slide assembly drives the camera assembly to move in the Z-axis direction to acquire magnified microscopic images at different positions of the detection module. The Z-axis slide assembly drives the camera assembly to find the optimal imaging position to acquire clear magnified microscopic images.
[0037] like Figure 3 In one embodiment of a pipeline-type formed element detection device with a microfluidic metering pump, an illumination component is further included, wherein the illumination component and the camera component are respectively located above or below the detection module.
[0038] like Figure 6 In one embodiment of a pipeline-type formed element detection method, the following steps are included: A30: a microfluidic metering pump drives a microscopic sample with a volume equal to VA to flow through a detection chamber; A40: a microscopic image of a sample equal to VA is captured; A50: AI identifies the formed element categories in the microscopic image and obtains the quantity of selected formed elements; A60: the content of formed elements per unit volume is obtained.
[0039] like Figure 8 In one embodiment of a pipeline-based formed element detection method, the method further includes step A10: cleaning the pipeline. For example... Figure 9Step A10 may further include step A11: pumping cleaning fluid into the detection chamber; or step A10 may further include step A12: pumping air into the detection chamber.
[0040] like Figure 7 In one embodiment of a pipeline-type formed element detection method, A20 is further included: a sample injection step; as shown... Figure 10 Step A20 above includes step A21, in which the microscopic sample is pumped into the buffer tank by a peristaltic pump, and in step A30, the microscopic sample is pumped from the buffer tank into the detection chamber by a microfluidic metering pump.
[0041] While this application has been described and illustrated with reference to preferred embodiments and several alternatives, it is not intended to be limited to the specific descriptions herein. Other alternatives or equivalent components may also be used to practice this application.
Claims
1. A pipeline-type formed element detection device with a microfluidic metering pump, characterized in that, Includes a detection module, drainage pipe, inlet pipe, sample inlet, camera assembly, and microfluidic metering pump; The detection module includes an inlet, a detection chamber, and an outlet; the detection chamber is connected to the inlet and outlet. The inlet pipe is connected to the inlet port; the outlet pipe is connected to the outlet port; the sample inlet head is connected to the inlet pipe; The camera assembly is used to capture images of microscopically examined samples inside the inspection chamber; A microfluid metering pump is connected in series in the inlet or outlet pipe; The microfluidic metering pump is used to measure the volume (VA) of the microscopic sample flowing through the detection chamber. The camera component is used to capture microscopic images of the microscopically examined sample with a volume equal to VA.
2. The pipeline-type formed element detection device with microfluidic metering pump according to claim 1, characterized in that, It also includes an inlet pump and a buffer tank; the sample inlet head, inlet pump, and buffer tank are connected in series in the inlet pipeline. The inlet pump is used to drive external microscopic samples, air, or cleaning liquid into the buffer tank.
3. The pipeline-type formed element detection device with microfluidic metering pump according to claim 2, characterized in that, The upper part of the buffer box includes a pressure balancing opening, which is connected to a pressure balancing valve.
4. The pipeline-type formed element detection device with microfluidic metering pump according to any one of claims 2, characterized in that, Includes any one of the following technical features: TA1: The inlet pump is a peristaltic pump; TA2: The microfluidic metering pump is a piezoelectric pump.
5. The pipeline-type formed element detection device with microfluidic metering pump according to claim 3, characterized in that, It also includes a drain hose and a waste liquid collection device, wherein one end of the drain hose is connected to the drain pipe and the other end is connected to the waste liquid collection device.
6. The pipeline-type formed element detection device with microfluidic metering pump according to claim 1, characterized in that, It also includes an illumination component, which is located above or below the detection module, respectively, as is the camera component.
7. A pipeline-type formed element detection method, characterized in that, Including steps A30: A microfluidic metering pump drives a microscopic sample with a volume equal to VA to flow through the detection chamber; A40: Take microscopic images of samples equal to VA; A50: AI identifies the categories of formed elements in the microscopic image and obtains the quantity of selected formed elements; A60: Obtain the content of formed elements per unit volume.
8. The pipeline-type formed element detection method according to claim 7, characterized in that, It also includes, A10: Pipeline cleaning steps; Step A10 also includes any one of the following technical features: TB1: also includes step A11: pumping cleaning fluid into the detection chamber; TB2: also includes step A12: pumping air into the detection chamber.
9. The pipeline-type formed element detection method according to claim 7, characterized in that, It also includes A20: the injection step; In step A20, the microscopic sample is pumped into the buffer box using a peristaltic pump.
10. The pipeline-type formed element detection method according to claim 7, characterized in that, In step A30, the microscopic sample is pumped from the buffer tank into the detection chamber using a microfluidic metering pump.