Ganoderma lucidum strain culturing and screening device and method thereof

Abstract

The invention relates to the technical field of ganoderma lucidum culture, in particular to a ganoderma lucidum strain culture screening device and a method thereof.The ganoderma lucidum strain culture screening device comprises a culture box, a cabinet door hinged to an opening of the culture box and a base installed at the bottom end of the culture box, a frame is installed at the inner end of the culture box, and three transverse plates are installed at the inner end of the frame; an environment regulation and control assembly is arranged at the inner end of the culture box, inlet and outlet holes are formed in the two sides of the three transverse plates and the upper ends of the vertical plates correspondingly, a plurality of sealing curtains are arranged at the inner ends of the inlet and outlet holes, a circulating spiral pipe is embedded in the inner bottom end of the culture box, and an elastic sleeve is connected to the circulating spiral pipe in a rolling and sleeving mode; the inner ends of the elastic sleeves are provided with a plurality of groups of magnetic blocks, and the outer wall of one side of each elastic sleeve is provided with a culture vessel, it is ensured that strains fully respond to differential environment regulation and control, the whole process from germination, growth and differentiation to domestication, rejuvenation and screening is continuous and controllable, and the problems that traditional static culture is low in efficiency and tedious in operation are solved.

Description

Technical Field

[0001] This invention relates to the field of Ganoderma lucidum cultivation technology, specifically to a Ganoderma lucidum strain cultivation and screening device and method. Background Technology

[0002] Ganoderma lucidum strains refer to pure cultures of Ganoderma lucidum mycelium that can be used for artificial cultivation and have stable genetic traits. They serve as the "seed source" for the growth of Ganoderma lucidum mycelium and the differentiation of fruiting bodies. They need to be obtained through processes such as isolation, purification, and rejuvenation. Their core characteristics are high purity, strong activity, and genetic stability. They have a wide range of applications, including being used as inoculation material for large-scale cultivation of Ganoderma lucidum, and the fruiting bodies produced can be used in medicine or processed into health products. They can also be used directly for mycelial fermentation to produce active ingredients such as polysaccharides and triterpenes, which are applied in the fields of medicine, food, and cosmetics. Traditional cultivation methods have significant drawbacks: they mostly employ static stratified cultivation, requiring frequent manual transfer of culture vessels to adapt to different growth stages. This is not only cumbersome and inefficient, but also prone to contamination by other microorganisms and environmental fluctuations due to opening the container, causing mechanical damage to the mycelium. Furthermore, the uniform cultivation environment makes it impossible to achieve differentiated control at different stages, making it difficult to meet the growth requirements of the strain at each stage. This results in low standardization of cultivation and poor screening results. Therefore, it is necessary to propose a Ganoderma lucidum strain cultivation and screening device and method. Summary of the Invention

[0003] To address the problems in the prior art, this invention provides a Ganoderma lucidum strain culture and screening device and method.

[0004] The technical solution adopted by the present invention to solve its technical problem is: a Ganoderma lucidum strain culture and screening device, including a culture box, a cabinet door hinged to the opening of the culture box, and a base installed at the bottom of the culture box. A frame is installed inside the culture box, three horizontal plates are installed inside the frame, a vertical plate is installed in the middle of the three horizontal plates, and an environmental control component is provided inside the culture box. The three horizontal plates are provided with inlet and outlet holes on both sides and the upper part of the vertical plates respectively. Several sealing curtains are provided inside the inlet and outlet holes. A circulating spiral tube is embedded in the bottom of the incubator. An elastic sleeve is rolled on the circulating spiral tube. Multiple sets of magnetic blocks are provided inside the elastic sleeve. A culture vessel is installed on one side of the outer wall of the elastic sleeve. The base has a drive groove in the middle. A reduction motor is installed on one side of the inner end of the drive groove. Both ends of the drive groove are rotatably connected to a shaft. One of the shafts is connected to the output end of the reduction motor. Each shaft wall is fixedly connected to a pulley. A transmission belt is connected between the two pulleys. Each shaft wall is fixedly connected to a spool. A transmission line is connected between the spools. Each transmission line is provided with multiple sets of magnetic collars, and the diameter of the transmission line matches the inner diameter of the circulating spiral tube.

[0005] Specifically, a transparent observation window is embedded in the middle of the cabinet door, and a handle is installed on the outer edge of the cabinet door.

[0006] Specifically, several of the aforementioned sealing curtains are made of silicone material.

[0007] Specifically, the culture vessel has a circular groove at its open end.

[0008] Specifically, the outer wall of the circulating spiral tube is provided with multiple rolling grooves, and the inner end of the elastic sleeve is provided with several rolling balls, which are rolled and stuck in the rolling grooves.

[0009] Specifically, the two sides of the frame are respectively set as a downward reinforcement zone and an upward cultivation zone. The upward cultivation zone includes a germination zone, a growth zone and a differentiation zone from bottom to top. The downward reinforcement zone includes a low temperature acclimatization zone, a nutrient supply zone and an activity screening zone from top to bottom. The shaded parts at the top and bottom of both sides are screening constant temperature zones.

[0010] Specifically, a tensioning assembly is provided at the inner end of the drive groove near the transmission line.

[0011] A method for culturing and screening Ganoderma lucidum strains includes the following steps: S1 Inoculum Loading and Pre-inspection: The Ganoderma lucidum inoculum to be screened is inoculated into the culture vessel. A hydrophobic PTFE breathable membrane is laid at the circular groove of the vessel and sealed. Check whether the ball bearings in the elastic sleeve are completely inserted into the rolling groove of the circulating spiral tube to ensure smooth rolling without jamming. At the same time, check the opposite polarity adsorption state of the magnetic block in the elastic sleeve and the magnetic ring on the transmission line to ensure that the adsorption is accurate and without misalignment.

[0012] S2 Zoned Environmental Parameter Setting and Driving: Start and close the cabinet door of incubator 1, start the environmental control component, and set the temperature, humidity, and gas concentration parameters for the upward culture zone (germination zone, growth zone, differentiation zone) and the downward reinforcement zone (low temperature acclimatization zone, nutrient supply zone, and activity screening zone), respectively; start the geared motor, which drives the pulley to rotate through the drive shaft, causing the thread wheel and transmission line to move slowly along the inner hole of the circulating spiral tube, and using magnetic adsorption to pull the elastic sleeve and culture vessel to slide along the outer wall of the spiral tube.

[0013] S3 Spiral Circulation Culture and Enhancement Treatment: The culture vessel, along with the elastic sleeve, passes through each functional segment of the upward culture zone in sequence along the circulating spiral tube, completing the germination of the strain, mycelial growth, and differentiation of fruiting body primordia; then it enters the downward enhancement zone, where it successively completes low-temperature stress resistance acclimatization and nutrient replenishment rejuvenation. Relying on the structural design of the spiral tube, the residence time of the culture vessel in each functional zone is extended, ensuring that the strain fully responds to environmental regulation at each stage.

[0014] S4 Constant Temperature Screening and Finished Product Harvesting: The culture vessels are moved to the constant temperature screening zones at both ends of the circulating spiral tube. Based on the colony morphology, mycelial vigor, and stress resistance of the Ganoderma lucidum strains, high-quality target strains are screened out. The culture vessels that pass the screening are harvested, and the vessels that do not meet the standards are cleaned up. The pre-tension of the transmission line is adjusted by the tensioning component, which improves the stability of the transmission line during transport.

[0015] One of the beneficial effects of this invention is that it enables fully automated, cyclical cultivation of Ganoderma lucidum strains, significantly improving cultivation efficiency and standardization. Through the combination of a circulating spiral tube and a magnetic drive system, the culture vessel can automatically flow along a preset trajectory through the functional sections of the upward cultivation zone and the downward enhancement zone, eliminating the need for manual transfer. This avoids contamination and environmental fluctuations caused by frequent opening of the container, and also reduces the risk of mechanical damage to the mycelium.

[0016] The second beneficial effect of this invention is that the spiral tube structure prolongs the residence time of the strains in each region. Combined with the partitioned isolation design of the silicone sealing curtain, it ensures that the strains fully respond to the differentiated environmental regulation. The entire process from germination, growth, differentiation to domestication, rejuvenation, and screening is continuously controllable, solving the pain points of low efficiency and cumbersome operation of traditional static culture. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0018] Figure 1 This is a schematic diagram of the structure of a Ganoderma lucidum strain culture and screening device provided by the present invention; Figure 2 This is a schematic cross-sectional view of the overall structure of a Ganoderma lucidum strain cultivation and screening device provided by the present invention; Figure 3 This invention provides a Ganoderma lucidum strain culture and screening device. Figure 2 Enlarged structural diagram at point A in the middle; Figure 4 A schematic diagram of the circulating spiral tube structure of a Ganoderma lucidum strain culture and screening device provided by the present invention; Figure 5 This is a schematic diagram of the frame planar structure of a Ganoderma lucidum strain cultivation and screening device provided by the present invention; Figure 6 This is a schematic diagram of the transmission line structure of a Ganoderma lucidum strain cultivation and screening device provided by the present invention; Figure 7 This invention provides a Ganoderma lucidum strain culture and screening device. Figure 6 Enlarged structural diagram at point B.

[0019] In the diagram: 1. Incubator; 2. Cabinet door; 3. Base; 10. Frame; 11. Horizontal plate; 12. Vertical plate; 13. Sealing curtain; 14. Environmental control component; 15. Circulating spiral tube; 16. Elastic sleeve; 160. Ball bearing; 161. Magnetic block; 17. Culture vessel; 21. Transparent observation window; 30. Tensioning component; 301. Drive groove; 31. Rotating shaft; 32. Pulley; 33. Transmission belt; 34. Thread pulley; 35. Transmission line; 105. Rolling groove; 110. Downward reinforcement zone; 111. Upward culture zone; 310. Gear motor; 350. Magnetic collar. Detailed Implementation

[0020] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0021] like Figures 1-7 As shown, the present invention provides the following technical solution: Example 1: A Ganoderma lucidum strain cultivation and screening device includes an incubator 1, a cabinet door 2 hinged to the opening of the incubator 1, and a base 3 installed at the bottom of the incubator 1. A frame 10 is installed inside the incubator 1, three horizontal plates 11 are installed inside the frame 10, and a vertical plate 12 is installed in the middle of the three horizontal plates 11. An environmental control component 14 is provided inside the incubator 1. A transparent observation window 21 is embedded in the middle of the cabinet door 2, and a handle is installed on the outer edge of the cabinet door 2.

[0022] The environmental control component 14 includes zoned gas nozzles, ultrasonic atomizers, condensation dehumidification modules, O2 / CO2 dual-parameter sensors, zoned temperature control heating elements, cooling fans, and sterile circulating fans and filter membrane components.

[0023] When in use, open cabinet door 2, place the culture dish 17 inoculated with Ganoderma lucidum spores in layers on the horizontal plate 11 of frame 10, close cabinet door 2 and lock it; start the environmental control component 14 in incubator 1, set the appropriate temperature, humidity and gas concentration parameters, and monitor the growth status of the spores in real time through the transparent observation window 21 on cabinet door 2; after the spores have been cultured to the target stage, open cabinet door 2 and take out culture dish 17 to complete the static culture operation of Ganoderma lucidum spores.

[0024] Example 2: The technical solution of this example that differs from that of Example 1 includes: inlet and outlet holes are respectively opened on both sides of the three horizontal plates 11 and the upper end of the vertical plate 12. Several sealing curtains 13 are provided inside the inlet and outlet holes. A circulating spiral tube 15 is embedded in the bottom of the incubator 1. An elastic sleeve 16 is rolled on the circulating spiral tube 15. Multiple sets of magnetic blocks 161 are provided inside the elastic sleeve 16. A culture vessel 17 is installed on one side of the outer wall of the elastic sleeve 16. A drive groove 301 is provided in the center of the base 3. A geared motor 310 is installed on one side of the inner end of the drive groove 301. A rotating shaft 31 is rotatably connected to both ends of the drive groove 301. One of the rotating shafts 31 is connected to the output end of the geared motor 310. Pulleys 32 are fixedly connected to the shaft walls of the rotating shafts 31. A drive belt 33 is connected between the two pulleys 32. A spool 34 is fixedly connected to one end of the shaft wall of the rotating shafts 31. A drive line 35 is connected between the spools 34. Each drive line 35 is equipped with a drive belt. There are multiple sets of magnetic collars 350, and the diameter of the transmission line 35 is matched with the inner diameter of the circulating spiral tube 15. Several sealing curtains 13 are made of silicone material. The opening end of the culture vessel 17 is provided with a circular groove. Multiple rolling grooves 105 are opened on the outer wall of the circulating spiral tube 15. Several balls 160 are rolled and embedded in the inner end of the elastic sleeve 16, and the balls 160 are rolled and stuck in the rolling grooves 105. A tensioning component 30 is provided between the inner end of the drive groove 301 and the transmission line 35.

[0025] Among them, the geared motor 310 is connected to an external power supply via a PLC controller; The magnetic collars 350 are all magnetically connected to several magnetic blocks 161; In use, the inoculated Ganoderma lucidum spawn is placed into the culture vessel 17. A hydrophobic PTFE breathable membrane is laid at the circular groove of the vessel and sealed. Then, the culture vessel 17 is fixedly connected to the elastic sleeve 16. Check whether the ball bearing 160 inside the elastic sleeve 16 is stuck in the rolling groove 105 of the circulating spiral tube 15, and ensure that the magnetic block 161 inside the elastic sleeve 16 and the magnetic collar 350 on the transmission line 35 are precisely attracted by opposite poles. Close the cabinet door 2 and start the reduction motor 310. The reduction motor 310 drives the pulley 32 to rotate through the drive shaft 31, which drives the thread wheel 34 and the transmission line 35 to move along the inner hole of the circulating spiral tube 15. The elastic sleeve 16 and the culture vessel 17 are tractioned by magnetic adsorption to slide along the outer wall of the spiral tube. At the same time, the environmental control component 14 is started, and the local environment inside the incubator 1 is kept stable by the silicone sealing curtain 13. During this period, the pretension of the transmission line 35 is adjusted by the tensioning component 30 to prevent the transmission line 35 from slipping.

[0026] Example 3: The technical solution that differs from Example 1 in this example includes: the two sides of the frame 10 are respectively set as a downward strengthening zone 110 and an upward cultivation zone 111. The upward cultivation zone 111 includes a germination zone, a growth zone and a differentiation zone from bottom to top. The downward strengthening zone 110 includes a low temperature acclimatization zone, a nutrient supply zone and an activity screening zone from top to bottom. The shaded parts at the top and bottom of both sides are screening constant temperature zones.

[0027] in, Germination zone: This zone activates the dormant state of Ganoderma lucidum spores and promotes spore germination to form mycelium. It requires a high-humidity, suitable-temperature, and sufficient-oxygen environment. Growth zone: To promote rapid mycelial extension and full coverage of the culture medium, it is necessary to maintain an environment with suitable temperature, medium to high humidity, and slight CO2 stress. Differentiation zone: Induces mycelium to shift from vegetative growth to reproductive growth, forming fruiting body primordia, requiring a fluctuating temperature, moderate humidity, low oxygen and high CO2 stress environment; Low-temperature acclimatization zone: This zone aims to induce strains to tolerate low-temperature environments, enhance their stress resistance, and eliminate weak strains. It requires maintaining a stress environment of low temperature, low humidity, and normal air pressure.

[0028] Nutritional replenishment area: To repair mycelial damage caused by low temperature stress and rejuvenate the strain, it is necessary to maintain a nutrient supply environment with suitable temperature, medium to high humidity, and a small amount of CO2 and sufficient O2.

[0029] Screening area (including constant temperature screening area): High-quality strains are screened using a standardized, stable environment, requiring the maintenance of a uniform environment with constant temperature, moderate humidity, and normal air pressure. In use, the environmental control component 14 is activated to set the differentiated environmental parameters for the germination zone, growth zone, and differentiation zone of the upward culture zone 111, and the low-temperature acclimatization zone, nutrient supply zone, and activity screening zone of the downward enhancement zone 110. The drive system is activated to pull the culture vessel 17 along the circulating spiral tube 15 into the upward culture zone 111, where the germination of the strain, mycelial growth, and differentiation of the fruiting body primordia are completed in sequence. Subsequently, the culture vessel 17 enters the downward enhancement zone 110, where the strain's resistance to stress is improved through low-temperature acclimatization and the strain is rejuvenated through nutrient supply. Finally, it moves to the screening constant temperature zone at the upper and lower ends on both sides, where high-quality Ganoderma lucidum strains are selected based on colony morphology and mycelial robustness, thus completing the full-cycle culture and screening process.

[0030] A method for culturing and screening Ganoderma lucidum strains includes the following steps: S1. Inoculation and Pre-inspection: The Ganoderma lucidum strain to be screened is inoculated into the culture vessel 17. A hydrophobic PTFE breathable membrane is laid at the circular groove of the vessel and sealed. Check whether the ball bearing 160 in the elastic sleeve 16 is completely inserted into the rolling groove 105 of the circulating spiral tube 15 to ensure smooth rolling without jamming. At the same time, check the opposite polarity adsorption state of the magnetic block 161 in the elastic sleeve 16 and the magnetic collar 350 on the transmission line 35 to ensure that the adsorption is accurate and without deviation or misalignment.

[0031] S2 Zoned Environmental Parameter Setting and Driving: Start and close cabinet door 2 of incubator 1, start environmental control component 14, and set temperature, humidity, and gas concentration parameters for the upward culture zone 111 germination zone, growth zone, differentiation zone and the downward strengthening zone 110 low temperature acclimatization zone, nutrient supply zone and activity screening zone respectively; start reduction motor 310, drive pulley 32 to rotate through drive shaft 31, drive thread pulley 34 and transmission line 35 to move slowly along the inner hole of circulating spiral tube 15, and use magnetic adsorption to pull elastic sleeve 16 and culture vessel 17 to slide along the outer wall of spiral tube.

[0032] S3 Spiral Circulation Culture and Enhancement Treatment: The culture vessel 17, along the elastic sleeve 16, passes through each functional segment of the upward culture zone 111 in sequence along the circulating spiral tube 15, completing the germination of the strain, mycelial growth, and differentiation of fruiting body primordia; then it enters the downward enhancement zone 110, where it successively completes low-temperature stress resistance acclimatization and nutrient replenishment rejuvenation. Relying on the structural design of the spiral tube, the residence time of the culture vessel 17 in each functional zone is extended to ensure that the strain fully responds to environmental regulation at each stage.

[0033] S4 Constant Temperature Screening and Finished Product Harvesting: The culture vessel 17 is moved to the constant temperature screening zone at both ends of the circulating spiral tube 15. Based on the colony morphology, mycelial vigor, and stress resistance of the Ganoderma lucidum strains, high-quality target strains are screened out. The culture vessels 17 that have passed the screening are harvested, and the vessels that have not met the standards are cleaned up. The pre-tension of the transmission line 35 is adjusted by the tensioning component 30, which improves the stability of the transmission line 35 during transportation.

[0034] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of protection claimed by the present invention. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Ganoderma lucidum strain culture and screening device, comprising an incubator (1) and a cabinet door (2) hinged to the opening of the incubator (1) and a base (3) installed at the bottom of the incubator (1), wherein a frame (10) is installed at the inner end of the incubator (1), three horizontal plates (11) are installed at the inner end of the frame (10), a vertical plate (12) is installed in the middle of the three horizontal plates (11), and an environmental control component (14) is provided at the inner end of the incubator (1); Its features are, The three horizontal plates (11) are provided with inlet and outlet holes on both sides and the upper end of the vertical plate (12). Several sealing curtains (13) are provided inside the inlet and outlet holes. A circulating spiral tube (15) is embedded in the bottom of the incubator (1). An elastic sleeve (16) is rolled on the circulating spiral tube (15). Multiple sets of magnetic blocks (161) are provided inside the elastic sleeve (16). A culture vessel (17) is installed on the outer wall of one side of the elastic sleeve (16). The base (3) has a drive groove (301) in the middle. A geared motor (310) is installed on one side of the inner end of the drive groove (301). Both ends of the drive groove (301) are rotatably connected to a rotating shaft (31). One of the rotating shafts (31) is connected to the output end of the geared motor (310). The shaft wall of the rotating shaft (31) is fixedly connected to a pulley (32). A transmission belt (33) is connected between the two pulleys (32). One end of the shaft wall of the rotating shaft (31) is fixedly connected to a spool (34). A transmission line (35) is connected between the spools (34). Multiple sets of magnetic collars (350) are provided on the transmission line (35). The diameter of the transmission line (35) matches the inner diameter of the circulating spiral tube (15).

2. The Ganoderma lucidum strain cultivation and screening device according to claim 1, characterized in that: A transparent observation window (21) is embedded in the middle of the cabinet door (2), and a handle is installed on the outer edge of the cabinet door (2).

3. The Ganoderma lucidum strain cultivation and screening device according to claim 1, characterized in that: Several of the aforementioned sealing curtains (13) are made of silicone material.

4. The Ganoderma lucidum strain cultivation and screening device according to claim 1, characterized in that: The culture vessel (17) has a circular groove at its open end.

5. The Ganoderma lucidum strain cultivation and screening device according to claim 1, characterized in that: The outer wall of the circulating spiral tube (15) is provided with multiple rolling grooves (105), and the inner end of the elastic sleeve (16) is provided with several rolling balls (160), and the rolling balls (160) are all rolled and stuck in the rolling grooves (105).

6. The Ganoderma lucidum strain cultivation and screening device according to claim 1, characterized in that: The frame (10) is configured with a downward strengthening zone (110) and an upward cultivation zone (111) on both sides respectively. The upward cultivation zone (111) includes a germination zone, a growth zone and a differentiation zone from bottom to top. The downward strengthening zone (110) includes a low temperature acclimatization zone, a nutrient supply zone and an activity screening zone from top to bottom. The shaded parts at the top and bottom of both sides are screening constant temperature zones.

7. The Ganoderma lucidum strain cultivation and screening device according to claim 1, characterized in that: A tensioning assembly (30) is provided between the inner end of the drive groove (301) and the transmission line (35).

8. A method for culturing and screening Ganoderma lucidum strains, wherein the method is implemented using the Ganoderma lucidum strain culturing and screening device according to any one of claims 1-7, characterized in that, Includes the following steps: S1. Inoculation and Pre-inspection: The Ganoderma lucidum strain to be screened is inoculated into the culture vessel (17). A hydrophobic PTFE breathable membrane is laid at the circular groove of the vessel and sealed. Check whether the ball (160) in the elastic sleeve (16) is completely inserted into the rolling groove (105) of the circulating spiral tube (15) to ensure smooth rolling without jamming. At the same time, check the opposite polarity adsorption state of the magnetic block (161) in the elastic sleeve (16) and the magnetic collar (350) on the transmission line (35) to ensure that the adsorption is accurate and without deviation or misalignment. S2 partition environment parameter setting and driving: start and close the cabinet door (2) of incubator 1, start the environment control component (14), and set the temperature, humidity and gas concentration parameters of the upward culture zone (111) germination zone, growth zone, differentiation zone and the downward strengthening zone (110) low temperature acclimatization zone, nutrient supply zone and activity screening zone respectively; start the geared motor (310), drive the pulley (32) to rotate through the drive shaft (31), drive the thread wheel (34) and transmission line (35) to move slowly along the inner hole of the circulating spiral tube (15), and use magnetic adsorption to pull the elastic sleeve (16) and culture vessel (17) to slide along the outer wall of the spiral tube; S3 Spiral Circulation Culture and Enhancement Treatment: The culture vessel (17) passes through each functional segment of the upward culture zone (111) along the circulating spiral tube (15) with the elastic sleeve (16), completing the germination of the strain, mycelial growth and differentiation of the fruiting body primordia; then it enters the downward enhancement zone (110), completing the low temperature stress resistance acclimatization and nutrient replenishment rejuvenation in sequence. The structure design of the spiral tube extends the residence time of the culture vessel (17) in each functional zone, ensuring that the strain fully responds to the environmental regulation at each stage; S4 Constant Temperature Screening and Finished Product Harvesting: The culture vessel (17) is moved to the constant temperature screening zone at both ends of the circulating spiral tube (15). Based on the colony morphology, mycelial robustness and stress resistance of the Ganoderma lucidum strain, high-quality target strains are screened out. The culture vessels (17) that have passed the screening are harvested. The vessels that have not met the standards are cleaned up. The pre-tightening force of the transmission line (35) is adjusted by the tensioning component (30), which improves the stability of the transmission line (35) during transportation.

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