Aged muscle repairing composition and application

Abstract

The invention provides a senescent muscle repairing composition and application, and belongs to the technical field of cosmetics. Comprising polyunsaturated fatty acid, carotenoid, peptides, polydeoxyribonucleotide PDRN and at least one algae extract selected from chlorella, spirulina, brown algae and red algae. According to the senescent muscle repairing composition prepared by the invention, through a carefully selected natural active component system, the composition has a synergistic effect from two major directions of promoting synthesis of ECM key components and inhibiting excessive degradation of ECM, and improves the structural quality of ECM, so that the steady state of skin ECM is effectively recovered, and the purposes of improving wrinkles and improving compactness and elasticity are achieved.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to an aging skin repair composition and its application. Background Technology

[0002] Skin aging is a complex biological process involving multiple levels, among which changes in the extracellular matrix are the direct structural basis for the appearance of aging skin (wrinkles, sagging, loss of elasticity). The skin's extracellular matrix (ECM) is mainly composed of macromolecules such as collagen (primarily type I and type III), elastin, hyaluronic acid, and proteoglycans, forming a three-dimensional network structure that provides the skin with physical support, elasticity, resilience, and moisturizing functions. The maintenance of its homeostasis depends on a delicate balance between synthesis by fibroblasts (biosynthesis) and degradation by enzyme systems such as matrix metalloproteinases (biodegradation).

[0003] This balance is disrupted as people age and environmental factors take effect: Reduced ECM synthesis: Fibroblast activity decreases, and the biosynthesis of collagen, elastin, and hyaluronic acid is significantly reduced.

[0004] ECM degradation is exacerbated: the activity of matrix metalloproteinase family members is increased, especially collagenase, gelatinase and elastase, leading to excessive degradation of functional ECM components. Decreased ECM structural quality: Abnormal cross-linking of collagen fibers (glycosylation), breakage and denaturation of elastic fibers, and a decrease in hyaluronic acid content and molecular weight lead to a loose ECM structure, weakened support, and reduced moisture retention capacity.

[0005] Therefore, effective anti-aging strategies must simultaneously focus on the two core directions of "promoting synthesis" and "inhibiting degradation" to restore the dynamic balance of the ECM. Current technologies often rely on single ingredients that focus only on one aspect (such as vitamin C alone promoting collagen synthesis, or a single MMP inhibitor), or use highly irritating or limited-source ingredients. Therefore, there is an urgent need to develop a natural-source solution that can systematically, gently, and synergistically regulate ECM homeostasis through multiple pathways. Summary of the Invention

[0006] The purpose of this invention is to propose an aging skin repair composition and its application. Through a carefully selected system of natural active ingredients, it works synergistically from two directions: "promoting the synthesis of key ECM components" and "inhibiting excessive ECM degradation", thereby improving the structural quality of ECM and effectively restoring the homeostasis of the skin's ECM, achieving the purpose of improving wrinkles, firmness and elasticity.

[0007] The technical solution of this invention is implemented as follows: This invention provides an aging skin repair composition comprising polyunsaturated fatty acids, carotenoids, peptides, polydeoxyribonucleic acid (PDRN), and at least one algal extract selected from Chlorella, Spirulina, brown algae, and red algae.

[0008] This combination of active ingredients can simultaneously: (a) Promotes the synthesis of key ECM components by skin fibroblasts; (b) Inhibit excessive degradation of ECM by matrix metalloproteinases; (c) Improve the structure and function of existing ECMs.

[0009] The following are the specific mechanisms by which each component plays a role in regulating ECM steady state: I. Promoting the synthesis of key ECM components Key active ingredients: peptides, PDRN, algal growth factor.

[0010] Mechanism of action: Signal transduction and activation: Specific signal peptides (such as palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7) can mimic or promote signal transduction pathways that promote collagen synthesis in vivo, activate fibroblasts, and upregulate the gene expression of type I and type III collagen and hyaluronic acid synthase. Polydeoxyribonucleotides promote cell proliferation and collagen production by providing cells with repair materials and signals.

[0011] Providing raw materials and energy for synthesis: Spirulina and Chlorella are rich in amino acids, trace elements, and vitamins (such as vitamin C precursors), which are essential substrates and cofactors for the synthesis of proteins such as collagen. Their polysaccharides and other components can also provide energy to cells and improve the metabolic environment of fibroblasts.

[0012] Activation of synthetic pathways: Active ingredients in certain red algae extracts can activate growth factor signaling pathways such as TGF-β, which are closely related to ECM synthesis.

[0013] II. Inhibiting excessive ECM degradation Key active ingredients: carotenoids, polyunsaturated fatty acids, and algal antioxidants.

[0014] Mechanism of action: Inhibition of MMP activity: Astaxanthin and fucoxanthin are potent natural MMP inhibitors. They can downregulate the induction of MMP gene expression by inflammatory factors (such as TNF-α and IL-1β) through anti-inflammatory and antioxidant pathways, and can directly or indirectly inhibit the activity of enzymes such as MMP-1, MMP-3, and MMP-9, thereby reducing the breakdown of collagen and elastin.

[0015] Reducing degradation triggers: Polyunsaturated fatty acids (such as Omega-3) have significant anti-inflammatory effects and can alleviate chronic low-grade inflammation in the skin, which is one of the main factors driving high MMP expression. Phycocyanin in Spirulina and fucoxanthin in brown algae also have strong anti-inflammatory capabilities.

[0016] Combating oxidative stress: Astaxanthin, fucoxanthin, and superoxide dismutase and flavonoids found in algae can effectively scavenge reactive oxygen species induced by ultraviolet radiation. ROS not only directly damage ECM components but are also key signaling molecules that activate MMPs and inflammatory pathways. Scavenging ROS inhibits degradation pathways from upstream.

[0017] III. Improve the structure and function of the ECM Key active ingredients: polyunsaturated fatty acids, hyaluronic acid (or components that promote its synthesis), and algal polysaccharides.

[0018] Mechanism of action: Optimize the lipid barrier and indirectly moisturize: Polyunsaturated fatty acids (such as those from flaxseed oil and fish oil) can supplement and optimize the lipid composition of the epidermis and sebum film, strengthen the skin barrier function, reduce transepidermal water loss, and provide a more stable hydration environment for the dermis, which is beneficial to the health of the ECM.

[0019] Direct and indirect moisturizing effects: Brown and red algae are rich in polysaccharides (such as fucoidan and carrageenan), which have excellent water-retention capabilities and can form a moisturizing film on the skin surface. At the same time, peptides and PDRN can promote the synthesis of endogenous hyaluronic acid, comprehensively improving the skin's water-holding capacity from the dermis to the epidermis, making the ECM function better in a well-hydrated environment and appear more plump.

[0020] Anti-glycation (potential): Some algae extracts (such as Chlorella) may have anti-glycation properties, which can help prevent abnormal cross-linking that causes collagen to harden and become brittle.

[0021] The synergistic effect of this invention: The aforementioned pathways do not operate independently, but rather form a positive synergistic cycle. For example, inhibiting degradation (pathway 2) reduces ECM loss, providing a more durable matrix basis for the synthesis of new components; while promoting synthesis (pathway 1) directly replenishes ECM capacity. A good skin barrier and moisturizing (pathway 3) provide an ideal microenvironment for fibroblast activity and ECM health. The selected natural ingredients generally have pleiotropic effects; for example, astaxanthin can both strongly inhibit MMPs and act as an antioxidant, while potentially indirectly promoting synthesis through anti-inflammatory effects, thereby achieving the most comprehensive homeostasis regulation with the simplest formulation.

[0022] As a further improvement of the present invention, the polyunsaturated fatty acid is selected from at least one of Omega-3 and Omega-6, wherein the Omega-3 is DHA or EPA; and the Omega-6 is linoleic acid or γ-linolenic acid.

[0023] As a further improvement of the present invention, the carotenoid is astaxanthin and / or fucoxanthin.

[0024] As a further improvement of the present invention, the peptide is selected from at least one of palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7.

[0025] As a further improvement of the present invention, the preparation method of the algae extract is as follows: algae powder is added to water, repeatedly frozen and thawed, a compound enzyme is added, ultrasonic enzymatic hydrolysis is performed, the enzyme is inactivated, filtered, and the filtrate is freeze-dried to obtain the algae extract.

[0026] As a further improvement of the present invention, the repeated freeze-thaw method involves freezing the mixture at -20 to -30°C, thawing it at room temperature, and repeating the operation 2-3 times.

[0027] As a further improvement of the present invention, the composite enzyme is cellulase and pectinase in a mass ratio of 5-10:2-3.

[0028] As a further improvement of the present invention, the mass ratio of algae powder to complex enzyme is 100:4-7.

[0029] As a further improvement of the present invention, the ultrasonic enzymatic hydrolysis power is 100-150W, the temperature is 30-40℃, and the time is 1-2h.

[0030] This invention further protects the use of the above-mentioned aging skin repair composition in cosmetics for anti-wrinkle and anti-aging skin.

[0031] The present invention has the following beneficial effects: The core mechanism targeting ECM homeostasis: This invention is not limited to a single aging phenomenon, but directly targets the structural root cause of skin aging—ECM imbalance. Through a scientific strategy of "promoting synthesis" and "inhibiting degradation" at the same time, it achieves root-cause and systemic anti-aging.

[0032] Multi-pathway natural synergy: Carefully selected polyunsaturated fatty acids, carotenoids, peptides, PDRN and specific algae, these natural ingredients work synergistically through different but complementary molecular mechanisms to maintain ECM health, with effects superior to the simple sum of single ingredients or chemically synthesized ingredients.

[0033] Balancing immediate and long-term effects: The moisturizing and antioxidant ingredients in the formula can quickly improve the appearance of the skin (such as brightening and hydration), while mechanisms such as promoting collagen synthesis bring long-term firming and wrinkle-reducing effects, meeting the multi-level needs of anti-aging skincare.

[0034] High safety and good compatibility: All core active ingredients are derived from natural or biotechnology, with high biocompatibility and low risk of irritation. They are suitable for long-term use on all skin types, including sensitive skin, and are compatible with other anti-aging ingredients.

[0035] Sustainability and environmental protection: Algae, as one of the core ingredient sources, has the advantages of sustainable cultivation and abundant resources, which is in line with the development trend of green cosmetics. Detailed Implementation

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] Preparation example: Preparation of algal extracts The method is as follows: Add 10g of algae powder to 200mL of water, freeze the mixture at -25℃, thaw at room temperature, repeat the operation 3 times, add 0.5g of compound enzyme, the compound enzyme being cellulase and pectinase in a mass ratio of 7:2, sonicate at 125W, heat to 35℃, enzymatically hydrolyze for 2h, inactivate the enzyme, filter, freeze-dry the filtrate to obtain algae extract.

[0038] Example 1: An ECM Steady-State Repair and Anti-Aging Essence Formulation composition (weight percentage): Palmitoyl tripeptide-5 (signal peptide) 0.01%; PDRN (polydeoxyribonucleic acid) 0.5% Astaxanthin (derived from red algae extract) 0.1%; Fucoxanthin (derived from brown algae extract) 0.5%; Chlorella extract (rich in amino acids) 2.0%; Spirulina extract (rich in phycocyanin and GLA) 1.0%; Flaxseed oil (rich in Omega-3) 3.0%; Glycerin (humectant) 5.0%; 1,2-Pentanediol (moisturizing and preservative) 1.5%; Carbomer (thickener) 0.15%; Triethanolamine (pH adjuster), appropriate amount; Add deionized water to a concentration of 100%.

[0039] Preparation method: (1) Disperse carbomer in a portion of deionized water and stir until fully swollen.

[0040] (2) Dissolve glycerol, 1,2-pentanediol, PDRN, peptides, and algae extracts in the remaining water and mix well.

[0041] (3) Dissolve astaxanthin and fucoxanthin in flaxseed oil.

[0042] (4) Add the aqueous phase (step 2) to the colloid (step 1) while stirring, and slowly add the oil phase (step 3) to homogenize and emulsify.

[0043] (5) Adjust the pH to 5.5-6.0 with triethanolamine.

[0044] Comparative Example 1: The difference compared to Example 1 is that no algae extract was added.

[0045] Formulation composition (weight percentage): Palmitoyl tripeptide-5 (signal peptide) 0.01%; PDRN (polydeoxyribonucleic acid) 0.5% Astaxanthin (derived from red algae extract) 0.1%; Fucoxanthin (derived from brown algae extract) 0.5%; Flaxseed oil (rich in Omega-3) 3.0%; Glycerin (humectant) 5.0%; 1,2-Pentanediol (moisturizing and preservative) 1.5%; Carbomer (thickener) 0.15%; Triethanolamine (pH adjuster), appropriate amount; Add deionized water to a concentration of 100%.

[0046] Preparation method: (1) Disperse carbomer in a portion of deionized water and stir until fully swollen.

[0047] (2) Dissolve glycerol, 1,2-pentanediol, PDRN and peptides in the remaining water and mix well.

[0048] (3) Dissolve astaxanthin and fucoxanthin in flaxseed oil.

[0049] (4) Add the aqueous phase (step 2) to the colloid (step 1) while stirring, and slowly add the oil phase (step 3) to homogenize and emulsify.

[0050] (5) Adjust the pH to 5.5-6.0 with triethanolamine.

[0051] Example 2: An ECM Steady-State Repairing Anti-Aging Face Cream Formulation composition (weight percentage): Acetyl hexapeptide-8 (neurotransmitter inhibitory peptide) 0.05%; Hydrolyzed red algae extract (provides moisturizing and nourishing properties) 3.0%; Brown algin (moisturizing film-forming agent) 0.5%; Ceramide NP (barrier strengthening) 0.5%; Squalane (similar to sebum) 4.0%; Shea butter (rich in fatty acids) 2.0%; Cetearyl alcohol olive oil ester (emulsifier) ​​3.0%; Glycerin 4.0%; Add deionized water to a concentration of 100%.

[0052] Preparation method: Prepared using conventional emulsification process.

[0053] Comparative Example 2: Unlike Example 2, this invention does not contain algae extract.

[0054] Formulation composition (weight percentage): Acetyl hexapeptide-8 (neurotransmitter inhibitory peptide) 0.05%; Brown algin (moisturizing film-forming agent) 0.5%; Ceramide NP (barrier strengthening) 0.5%; Squalane (similar to sebum) 4.0%; Shea butter (rich in fatty acids) 2.0%; Cetearyl alcohol olive oil ester (emulsifier) ​​3.0%; Glycerin 4.0%; Add deionized water to a concentration of 100%.

[0055] Preparation method: Prepared using conventional emulsification process.

[0056] Test Example 1: Moisturizing Effect Test One hundred individuals aged 20-52 (half male, half female), in good health, with regular lifestyles and no skin diseases, were randomly divided into five groups of 20 each. Each group used the products obtained in Examples 1-2 and Comparative Examples 1-2, respectively. The negative control group received an equal volume of deionized water. 2 mL of the product was applied to the entire face each time. The moisture content of the forehead was measured in an environment with a temperature of 25°C and a relative humidity of 60%, while the subjects remained calm. Two 1m × 1cm square experimental areas were marked on the forehead as test areas for the mask liquid. The skin moisture content was measured 2 hours after application using a skin moisture meter, and the results were recorded. Specific results are shown in Table 1.

[0057] Table 1 Moisturizing Effect Table

[0058] As can be seen from the table above, the products obtained in Examples 1-2 of this invention can significantly improve skin moisture content.

[0059] Test Example 2: Type III Collagen Synthesis Promotion Rate: The content of procollagen type III-peptide (PⅢP) in the supernatant of secreted type III collagen was determined using the "RIA-gnost PⅢP (PⅢP) Assay Kit". The anti-wrinkle effect of these samples was then evaluated by the type III collagen synthesis promotion rate.

[0060] Human fibroblasts cultured in DMEM medium containing 10% FBS were seeded into 24-well plates. After cell attachment, the medium was exchanged with DMEM medium containing 0.3% FBS and 250 μmol ascorbic acid magnesium phosphate. 2 µg of the products prepared in Examples 1-2 and Comparative Examples 1-2 were added to each plate. After three days of culture, the plates were centrifuged, and the supernatant was collected. The content of type III collagen in the supernatant was measured, and the promotion rate was calculated. The calculation formula is as follows: Promotion rate = (W1 - W0) / W0 * 100% W1: Type III collagen production after adding the test sample; W0: Type III collagen production without adding the test sample. The results are shown in Table 2.

[0061] Table 2

[0062] As shown in the table above, the products prepared in Examples 1-2 of this invention can promote the secretion of type III collagen by cells, significantly increase the production of type III collagen, thicken the extracellular matrix, and thus achieve an anti-wrinkle effect.

[0063] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A skin-repairing composition for aging, characterized in that, It includes polyunsaturated fatty acids, carotenoids, peptides, polydeoxyribonucleic acid (PDRN), and at least one algal extract selected from Chlorella, Spirulina, brown algae, and red algae.

2. The aging skin repair composition according to claim 1, characterized in that, The polyunsaturated fatty acid is selected from at least one of Omega-3 and Omega-6, wherein the Omega-3 is DHA or EPA; and the Omega-6 is linoleic acid or γ-linolenic acid.

3. The aging skin repair composition according to claim 1, characterized in that, The carotenoids are astaxanthin and / or fucoxanthin.

4. The aging skin repair composition according to claim 1, characterized in that, The peptides are selected from at least one of palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7.

5. The aging skin repair composition according to claim 1, characterized in that, The algae extract is prepared as follows: algae powder is added to water, repeatedly frozen and thawed, a compound enzyme is added, ultrasonic enzymatic hydrolysis is performed, the enzyme is inactivated, filtered, and the filtrate is freeze-dried to obtain the algae extract.

6. The aging skin repair composition according to claim 5, characterized in that, The method of repeated freeze-thaw cycles involves freezing the mixture at -20 to -30°C, thawing it at room temperature, and repeating the operation 2-3 times.

7. The aging skin repair composition according to claim 5, characterized in that, The compound enzyme is cellulase and pectinase in a mass ratio of 5-10:2-3.

8. The aging skin repair composition according to claim 5, characterized in that, The mass ratio of algae powder to compound enzyme is 100:4-7.

9. The aging skin repair composition according to claim 5, characterized in that, The ultrasonic enzymatic hydrolysis is performed at a power of 100-150W, a temperature of 30-40℃, and a time of 1-2 hours.

10. The use of an aging skin repair composition as described in any one of claims 1-9 in a cosmetic for anti-wrinkle and anti-aging skin.

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