Flow type sheath fluid clean circulation and bubble suppression device

Abstract

The invention relates to the technical field of flow cytometry sheath fluid circulation, in particular to a flow sheath fluid clean circulation and bubble suppression device, which comprises a control outer box body, a laser detection receiving unit, a sheath fluid wrapping cylinder, a cell sample impact unit, a sheath fluid multi-stage filtering assembly and a sheath fluid vacuum treatment unit, one end of the sheath fluid multi-stage filtering assembly is installed on the lower side of the sheath fluid wrapping barrel, the sheath fluid multi-stage filtering assembly can extract and filter sheath fluid on the inner side of the sheath fluid wrapping barrel, the sheath fluid vacuum treatment unit is connected with the end, away from the sheath fluid wrapping barrel, of the sheath fluid multi-stage filtering assembly, and the sheath fluid vacuum treatment unit can conduct vacuumizing treatment on the sheath fluid on the inner side. The sheath fluid multi-stage filtering assembly can suck detected sheath fluid into the sheath fluid and filter the sheath fluid, then the sheath fluid vacuum treatment unit vacuumizes the filtered sheath fluid and eliminates bubbles generated in the sheath fluid, then the sheath fluid wrapping cylinder is refilled with the sheath fluid after filtering and bubble removal, and circulation treatment of the sheath fluid is completed.

Description

Technical Field

[0001] This invention relates to the technical field of sheath fluid circulation in flow cytometry, and in particular to a flow cytometry sheath fluid clean circulation and bubble suppression device. Background Technology

[0002] Flow cytometry is a technique used for cell counting, cell classification, biomarker detection, and protein engineering. It involves ejecting a suspension of fluorescently labeled or intrinsically fluorescent cells through a nozzle, creating a single-cell flow. A wavelength-tunable laser is used to irradiate and detect, record, and analyze the fluorescence of each cell individually. It can perform multi-parameter analysis of the physical or chemical characteristics of these cells in real time at a rate of thousands of cells per second. Its core lies in the encapsulation effect of the sheath fluid on the sample flow—the sheath fluid propels the sample flow through the detection zone in a laminar flow manner, ensuring that each cell passes through the laser focus, thereby obtaining high-resolution optical signals, which are then converted into quantum images or quantum graphics.

[0003] In flow cytometry, the internal sheath fluid is generally recycled together.

[0004] Traditional flow-through sheath fluid systems have the following drawbacks: Contamination by impurities: During the circulation process, the sheath fluid is prone to the introduction of particulate impurities (such as dust and cell debris) due to poor container sealing, air contact, or sample residue. These impurities can clog microfluidic channels or scatter the laser. Bubble interference: During sheath fluid circulation, pump operation, pipeline bends, or temperature changes can easily generate bubbles. If bubbles enter the detection area, they will block the laser path, causing false positive / false negative results, or even damaging optical components. Summary of the Invention

[0005] To reduce the impact of impurities and bubbles in the sheath fluid circulation on detection, this invention provides a flow-type sheath fluid clean circulation and bubble suppression device.

[0006] The present invention provides a flow-type sheath fluid cleaning and circulation and bubble suppression device, which adopts the following technical solution: including: Control the outer casing.

[0007] A laser detection receiving unit is installed inside the control enclosure. The laser detection receiving unit is capable of emitting laser light and detecting optical signals.

[0008] A sheath fluid encapsulation tube is installed inside the control outer casing and between the laser detection and receiving units.

[0009] A cell sample impact unit is installed inside the sheath fluid encapsulation tube. The laser from the laser detection receiving unit can penetrate the sheath fluid encapsulation tube to detect cells in the cell sample impact unit. The cell sample impact unit penetrates the inner wall of the control outer casing.

[0010] A multi-stage sheath fluid filtration assembly is provided, with one end of the assembly installed on the lower side of the sheath fluid encapsulation cylinder. The assembly is capable of extracting and filtering the sheath fluid inside the encapsulation cylinder and is installed inside the control enclosure.

[0011] It also includes a sheath fluid vacuum treatment unit, which is connected to the end of the sheath fluid multi-stage filtration assembly away from the sheath fluid encapsulation tube. The sheath fluid in the sheath fluid multi-stage filtration assembly is filled into the sheath fluid vacuum treatment unit, which can vacuum the sheath fluid on the inside. The sheath fluid vacuum treatment unit then fills the vacuum-treated sheath fluid back into the sheath fluid encapsulation tube.

[0012] Optionally, the sheath fluid multi-stage filtration assembly includes: A collection bottom tube is installed at the lower end of the sheath fluid encapsulation tube.

[0013] A primary power pumping pipe, wherein the pumping end of the primary power pumping pipe is installed at the bottom of the collection bottom cylinder.

[0014] The filter tube is connected at its upper end to the outlet end of the primary power pumping pipe. The primary power pumping pipe draws the sheath fluid from the bottom collection cylinder and then fills the filter tube. Multiple filter units are installed at equal intervals on the inner side of the filter tube.

[0015] It also includes an impurity collection component, which is installed on the outside of the filter processing cylinder and is capable of collecting impurities filtered by the filter unit.

[0016] Optionally, the sheath fluid vacuum treatment unit includes: A secondary power-driven liquid extraction tube, wherein the extraction end of the secondary power-driven liquid extraction tube is installed at the lower end of the collection bottom cylinder.

[0017] A vacuum processing cylinder is installed at the outlet end of a secondary power pumping pipe.

[0018] An overflow ring is coaxially mounted on the upper end of the vacuum treatment cylinder, and an end cap is provided on the upper side of the overflow ring.

[0019] A reflux storage ring is coaxially and fixedly sleeved on the outside of an overflow ring. The lower end of the end cap is fixed to the upper end of the reflux storage ring. A three-stage power suction pipe is installed on the bottom surface of the reflux storage ring. The other end of the three-stage power suction pipe is connected to the outer surface of the sheath fluid wrapping cylinder.

[0020] It also includes a vacuum tube, which is installed through the upper surface of the end cover and the upper end of the vacuum tube penetrates the top wall of the control outer casing.

[0021] Optionally, the filter unit is configured as a cone with a pointed top, and the lower end of the filter unit is connected to the filter processing cylinder. The outer ring surface of the filter processing cylinder is provided with an impurity discharge groove at the lower end of each filter unit. Impurities blocked by the filter unit enter the inner side of the impurity collection assembly through the impurity discharge groove.

[0022] Optionally, the upper surface of the filter unit is equipped with multiple spiral guide plates arranged in a spiral pattern, and the multiple spiral guide plates are evenly distributed in a circumferential array around the axis of the filter unit.

[0023] Optionally, the impurity collection component includes: An impurity storage cylinder is fixedly sleeved on the outside of the filter treatment cylinder. A discharge pipe is installed at the lower end of the impurity storage cylinder, and the other end of the discharge pipe penetrates the inner wall of the control outer box.

[0024] A drive frame is provided, which is located between the outside of the filter tube and the inside of the impurity storage tube.

[0025] It also includes an impurity annular cavity disk, the number of which is equal to the number of impurity discharge slots. The impurity annular cavity disk is fixedly sleeved on the outer surface of the impurity storage cylinder, the impurity discharge slots are located inside the impurity annular cavity disk, the outer ring surface of the impurity annular cavity disk is open, and an outer sealing cylinder is slidably sleeved on the outer ring surface of the impurity annular cavity disk. The outer sealing cylinder is fixed to the drive vertical frame, and the drive vertical frame drives the outer sealing cylinder to move up and down.

[0026] Optionally, an inner sealing cylinder is slidably inserted into the inner side of the upper end of the impurity ring cavity disk. The inner sealing cylinder is slidably sleeved on the outer surface of the filter treatment cylinder. A linkage ring disk is provided on the upper side of the inner sealing cylinder. The linkage ring disk is fixed to the drive vertical frame. The linkage ring disk is slidably sleeved on the outer surface of the filter treatment cylinder. An elastic telescopic rod is fixed on the bottom surface of the linkage ring disk. The lower end of the elastic telescopic rod is fixed to the upper surface of the inner sealing cylinder.

[0027] Optionally, as the inner and outer sealing cylinders move downwards with the drive vertical frame, the inner sealing cylinder first seals the impurity discharge trough, and as the drive vertical frame continues to move downwards, the outer sealing cylinder and the opening of the impurity annular cavity are misaligned.

[0028] Optionally, the inner ring surface of the bottom wall of the impurity ring cavity is located above the outer ring surface of its inner bottom wall, the inner bottom wall of the impurity ring cavity is located below the conical surface of the filter unit, the lower end of the impurity storage cylinder is an eccentric execution groove, and the eccentric opening at the lower end of the impurity storage cylinder is coaxial with the upper end of the impurity discharge pipe.

[0029] Optionally, an integral liquid replacement pipe is installed at the lower end of the collection bottom cylinder, and the other end of the integral liquid replacement pipe penetrates the inner wall of the control outer box.

[0030] In summary, the present invention has the following beneficial technical effects: This invention utilizes a multi-stage sheath fluid filtration assembly and a sheath fluid vacuum treatment unit. The multi-stage sheath fluid filtration assembly draws the detected sheath fluid into its interior for filtration, separating impurities from the sheath fluid. Then, the sheath fluid vacuum treatment unit vacuums the filtered sheath fluid to eliminate air bubbles generated within the sheath fluid. Finally, the filtered and bubble-removed sheath fluid is refilled into the sheath fluid encapsulation cylinder, completing the sheath fluid circulation process.

[0031] This invention utilizes the combined use of an impurity annular chamber, an outer sealing cylinder, an inner sealing cylinder, and an impurity storage cylinder. During the circulation process, the outer sealing cylinder blocks the outside of the impurity annular chamber, allowing impurities generated during the circulation filtration to enter the impurity annular chamber. The impurity annular chamber collects the filtered impurities. When it is necessary to clean the impurities in the impurity annular chamber, the drive frame first controls the inner sealing cylinder to block the impurity discharge channel, and then the drive frame continues to move downwards. The outer sealing cylinder is misaligned with the impurity annular chamber, allowing the impurities in the impurity annular chamber to be discharged through the impurity storage cylinder and the discharge pipe, ensuring that the filtration unit does not reduce its filtration efficiency due to increased filtration time.

[0032] In this invention, the sheath fluid is filled into the vacuum treatment cylinder by using the overflow ring, vacuum treatment cylinder and reflux storage ring in combination. The vacuum tube evacuates the sheath fluid in the vacuum treatment cylinder. After the liquid level of the sheath fluid inside the vacuum treatment cylinder is higher than the overflow ring, the liquid flows into the reflux storage ring in a thinner state, which fully eliminates the internal air bubbles and prevents the air bubbles from remaining when the liquid level is thick.

[0033] This invention, through the setting of a conical filter unit and a spiral guide plate, forms a spiral water flow under the guidance of the spiral guide plate after the sheath liquid comes into contact with the upper surface of the filter unit. The spiral water flow increases the impact on the surface of the filter unit and reduces the residue of impurities on the surface of the filter unit. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the overall structure in an embodiment of the present invention; Figure 2 This is a schematic diagram of the distribution of the laser detection receiving unit and the sheath fluid wrapping tube in an embodiment of the present invention; Figure 3 This is a schematic diagram of the distribution of the sheath fluid encapsulation tube and the sheath fluid multi-stage filtration assembly in an embodiment of the present invention; Figure 4 This is a schematic diagram of the sheath fluid vacuum treatment unit in an embodiment of the present invention; Figure 5 This is a schematic diagram of the distribution of the vacuum processing cylinder and the overflow ring disk in an embodiment of the present invention; Figure 6 This is a schematic diagram of the structure of the sheath fluid multi-stage filtration assembly in an embodiment of the present invention; Figure 7 This is a schematic diagram of the distribution of the filter unit and the spiral guide plate in an embodiment of the present invention; Figure 8 This is a schematic diagram of the distribution of the impurity ring cavity disk and the outer closed cylinder in an embodiment of the present invention.

[0035] Reference numerals: 1. Control outer casing; 2. Laser detection receiving unit; 3. Sheath fluid encapsulation cylinder; 4. Cell sample impact unit; 5. Sheath fluid multi-stage filtration assembly; 51. Collection bottom cylinder; 52. Primary power suction tube; 53. Filtration processing cylinder; 54. Filtration unit; 55. Impurity collection assembly; 551. Impurity storage cylinder; 552. Impurity discharge pipe; 553. Drive vertical frame; 554. Impurity annular cavity disc; 555. Outer sealing cylinder; 556. Inner sealing cylinder; 557. Linkage annular disc; 558. Elastic telescopic rod; 56. Impurity discharge groove; 57. Spiral guide plate; 58. Overall fluid replacement pipe; 6. Sheath fluid vacuum processing unit; 61. Secondary power suction tube; 62. Vacuum processing cylinder; 63. Overflow annular disc; 64. End cap; 65. Return storage annular disc; 66. Tertiary power suction tube; 67. Vacuum tube. Detailed Implementation

[0036] The following is in conjunction with the appendix Figures 1-8 The present invention will be described in further detail below.

[0037] This invention discloses a flow-type sheath fluid cleaning and circulation and bubble suppression device. For example... Figures 1-3 As shown, it includes a control outer casing 1, a laser detection and receiving unit 2, a sheath fluid encapsulation tube 3, a cell sample impact unit 4, a sheath fluid multi-stage filtration assembly 5, and a sheath fluid vacuum processing unit 6.

[0038] The laser detection receiving unit 2 is installed inside the control outer casing 1. The laser detection receiving unit 2 can emit laser and detect light signals. The sheath fluid wrapping tube 3 is installed inside the control outer casing 1 and between the laser detection receiving units 2. The cell sample impact unit 4 is installed through the inner side of the sheath fluid wrapping tube 3. The laser detection receiving unit 2 can emit laser and detect light signals. The laser of the laser detection receiving unit 2 can pass through the sheath fluid wrapping tube 3 to detect cells in the cell sample impact unit 4. The cell sample impact unit 4 penetrates the inner wall of the control outer casing 1.

[0039] In this embodiment, the cell sample impact unit 4 can impact a single sample into the sheath fluid inside the sheath fluid encapsulation tube 3. The cell sample impact unit 4 is located at one end outside the control outer casing 1 and is connected to the cell sample providing device.

[0040] In use, the laser detection receiving unit 2 emits a laser, which can pass through the sheath fluid encapsulation tube 3 to detect cell samples. The detection light signal part of the laser detection receiving unit 2 receives and transmits the reflected light signal, and records and displays the detection data.

[0041] One end of the sheath fluid multi-stage filtration assembly 5 is installed on the lower side of the sheath fluid encapsulation cylinder 3. The sheath fluid multi-stage filtration assembly 5 can extract and filter the sheath fluid inside the sheath fluid encapsulation cylinder 3. The sheath fluid multi-stage filtration assembly 5 is installed inside the control outer casing 1.

[0042] The sheath fluid vacuum treatment unit 6 is connected to the end of the sheath fluid multi-stage filtration assembly 5 away from the sheath fluid encapsulation cylinder 3. The sheath fluid in the sheath fluid multi-stage filtration assembly 5 is filled into the sheath fluid vacuum treatment unit 6. The sheath fluid vacuum treatment unit 6 can perform vacuum treatment on the inner sheath fluid. The sheath fluid vacuum treatment unit 6 then fills the sheath fluid encapsulation cylinder 3 with the vacuum-treated sheath fluid again.

[0043] like Figures 4-8 As shown, the sheath fluid multi-stage filtration assembly 5 includes a collection bottom cylinder 51, a primary power pumping pipe 52, a filtration treatment cylinder 53, and an impurity collection assembly 55.

[0044] The collecting bottom cylinder 51 is installed at the lower end of the sheath fluid encapsulation cylinder 3. The suction end of the primary power suction pipe 52 is installed at the bottom of the collecting bottom cylinder 51. The upper end of the filtration treatment cylinder 53 is connected to the discharge end of the primary power suction pipe 52. The primary power suction pipe 52 draws the sheath fluid from the collecting bottom cylinder 51 and fills it into the filtration treatment cylinder 53. Multiple filtration units 54 are installed at equal intervals on the inner side of the filtration treatment cylinder 53. The filtration units 54 filter the sheath fluid flowing above and block impurities on the upper side of the filtration units 54. The filtration precision of the multiple filtration units 54 gradually increases from top to bottom, and the impurities of different sizes are filtered in layers, so that there are no impurities in the sheath fluid discharged from the lower end of the filtration treatment cylinder 53.

[0045] The bottom of the collecting cylinder 51 is equipped with an integral fluid replacement pipe 58. The other end of the integral fluid replacement pipe 58 penetrates the inner wall of the control outer casing 1. An electromagnetic valve is installed inside the integral fluid replacement pipe 58. When closed, the sheath fluid cannot pass through the integral fluid replacement pipe 58. When the electromagnetic valve is opened, the used sheath fluid in the collecting cylinder 51 can be discharged, and then new sheath fluid can be filled in to complete the replacement of the sheath fluid.

[0046] The filter unit 54 is configured as a cone with a pointed top. The lower end of the filter unit 54 is connected to the filter processing cylinder 53. The outer ring surface of the filter processing cylinder 53 is provided with an impurity discharge groove 56 at the lower end of each filter unit 54. The impurities blocked by the filter unit 54 enter the inner side of the impurity collection assembly 55 through the impurity discharge groove 56.

[0047] During use, when the sheath fluid passes through the conical filter unit 54, impurities remain on the upper side of the filter unit 54. Due to the conical shape of the filter unit 54, the blocked impurities tend to move towards the outer ring side, reducing the blocking effect of impurities on the filter unit 54 and ensuring that the filter unit 54 can filter the sheath fluid at a high efficiency.

[0048] Multiple spiral guide plates 57 are installed on the upper surface of the filter unit 54 in a spiral arrangement, and the multiple spiral guide plates 57 are evenly distributed in a circumferential array around the axis of the filter unit 54.

[0049] During use, when the sheath fluid passes through the spiral guide plate 57 on the upper side of the filter unit 54, the sheath fluid forms a spiral water flow under the guidance of the spiral guide plate 57. The spiral water flow increases the impact on the surface of the filter unit 54, further reducing the residue of impurities on the surface of the filter unit 54.

[0050] The impurity collection component 55 is installed on the outside of the filter processing cylinder 53. It can collect impurities filtered by the filter unit 54 and prevent impurities from accumulating on the upper side of the filter unit 54, thus affecting the continuous filtration effect of the filter unit 54.

[0051] The impurity collection assembly 55 includes an impurity storage cylinder 551, a drive frame 553, and an impurity annular disk 554.

[0052] Impurity storage cylinder 551 is fixedly sleeved on the outside of filter processing cylinder 53. Impurity storage cylinder 551 is equipped with impurity discharge pipe 552 at the lower end. The other end of impurity discharge pipe 552 penetrates the inner wall of control outer box 1. Drive vertical frame 553 is set between the outside of filter processing cylinder 53 and the inside of impurity storage cylinder 551.

[0053] The number of impurity annular disks 554 and impurity discharge grooves 56 are equal. The impurity annular disks 554 are fixedly sleeved on the outer surface of the impurity storage cylinder 551. The impurity discharge grooves 56 are located inside the impurity annular disks 554. The outer annular surface of the impurity annular disks 554 is open. The inner annular surface of the inner bottom wall of the impurity annular disks 554 is located above the outer annular surface of its inner bottom wall. The inner bottom wall of the impurity annular disks 554 is located below the conical surface of the filter unit 54. The lower end of the impurity storage cylinder 551 is an eccentric conical groove. The eccentric opening at the lower end of the impurity storage cylinder 551 is coaxial with the upper end of the impurity discharge pipe 552.

[0054] In the sheath fluid filtration process, the impurities filtered by the filtration unit 54 are temporarily collected in the impurity annular disk 554 through the impurity discharge tank 56.

[0055] The drive frame 553 is driven by a drive telescopic cylinder, which is connected to the control outer housing 1. The drive telescopic cylinder drives the drive frame 553 to move up and down.

[0056] An outer sealing cylinder 555 is slidably sleeved on the outer ring surface of the impurity annular disk 554. The outer sealing cylinder 555 is fixed to the drive vertical frame 553. The drive vertical frame 553 drives the outer sealing cylinder 555 to move up and down. An inner sealing cylinder 556 is slidably inserted into the inner side of the upper end of the impurity annular disk 554. The inner sealing cylinder 556 is slidably sleeved on the outer surface of the filter treatment cylinder 53. A linkage annular disk 557 is provided on the upper side of the inner sealing cylinder 556. The linkage annular disk 557 is fixed to the drive vertical frame 553. The linkage annular disk 557 is slidably sleeved on the outer surface of the filter treatment cylinder 53. An elastic telescopic rod 558 is fixed on the bottom surface of the linkage annular disk 557. The lower end of the elastic telescopic rod 558 is fixed to the upper surface of the inner sealing cylinder 556.

[0057] The inner sealing cylinder 556 and the outer sealing cylinder 555 move downwards following the drive vertical frame 553. The inner sealing cylinder 556 first seals the impurity discharge groove 56. The drive vertical frame 553 continues to move downwards, and the outer sealing cylinder 555 is misaligned with the opening of the impurity ring cavity disk 554.

[0058] After a certain amount of impurities accumulate in the impurity annular cavity disk 554, the drive frame 553 controls the inner sealing cylinder 556 and the outer sealing cylinder 555 to move downwards. The inner sealing cylinder 556 first blocks the impurity discharge groove 56, and then the drive frame 553 continues to move downwards. The drive frame 553 pushes the elastic telescopic rod 558 to compress through the linkage annular disk 557. The outer sealing cylinder 555 is misaligned with the impurity annular cavity disk 554, so that the impurities in the impurity annular cavity disk 554 are discharged through the impurity storage cylinder 551 and the impurity discharge pipe 552, so that the filtration unit 54 will not reduce the filtration effect due to the increase of filtration time.

[0059] The sheath fluid vacuum treatment unit 6 includes a two-stage power pumping pipe 61, a vacuum treatment cylinder 62, an overflow ring disk 63, a reflux storage ring disk 65, and a vacuum pumping pipe 67.

[0060] The liquid extraction end of the secondary power extraction pipe 61 is installed at the lower end of the collection bottom cylinder 51, the vacuum treatment cylinder 62 is installed at the liquid outlet end of the secondary power extraction pipe 61, the overflow ring disk 63 is coaxially installed at the upper end of the vacuum treatment cylinder 62, the overflow ring disk 63 is provided with an end cap 64 on the upper side, the return storage ring disk 65 is coaxially fixedly sleeved on the outside of the overflow ring disk 63, the lower end of the end cap 64 is fixed to the upper end of the return storage ring disk 65, the inner bottom wall of the return storage ring disk 65 is located below the upper surface of the overflow ring disk 63, and when the liquid level is higher than the overflow ring disk 63, the liquid can flow into the return storage ring disk 65.

[0061] A three-stage power extraction pipe 66 is installed on the bottom surface of the reflux storage ring plate 65, and the other end of the three-stage power extraction pipe 66 is connected to the outer surface of the sheath fluid wrapping cylinder 3.

[0062] During use, as the secondary power pumping pipe 61 fills the sheath fluid into the vacuum treatment cylinder 62, the liquid level in the vacuum treatment cylinder 62 gradually increases. After the liquid level is higher than the upper surface of the overflow ring disk 63, the liquid flows through the overflow ring disk 63 into the return storage ring disk 65. Then, the tertiary power pumping pipe 66 fills the sheath fluid encapsulation cylinder 3 with the vacuumed sheath fluid in the return storage ring disk 65. At the same time, by controlling the liquid level in the vacuum treatment cylinder 62, the portion of the sheath fluid above the overflow ring disk 63 is kept in a thinner state. The air bubbles inside the thinner sheath fluid can be fully processed under vacuum, reducing the residual air bubbles in the sheath fluid and preventing the air bubbles inside from not being fully absorbed when the sheath fluid is in a thicker state under vacuum.

[0063] The vacuum tube 67 is installed through the upper surface of the end cover 64. The upper end of the vacuum tube 67 passes through the inner top wall of the control outer box 1. A vacuum pump is installed on the outside of the control outer box 1. The outer end of the vacuum tube 67 is connected to the vacuum pump. The vacuum pump evacuates the vacuum treatment cylinder 62 through the vacuum tube 67.

[0064] In this embodiment, variable frequency water pumps and one-way valves are installed in the primary power pumping pipe 52, the secondary power pumping pipe 61, and the tertiary power pumping pipe 66. The primary power pumping pipe 52, the secondary power pumping pipe 61, and the tertiary power pumping pipe 66 control the flow of sheath fluid. A liquid level detection controller is installed through the upper end of the end cap 64. The liquid level detection controller controls the pumping speed of the primary power pumping pipe 52, the secondary power pumping pipe 61, and the tertiary power pumping pipe 66 by detecting the liquid level height in the vacuum processing cylinder 62, ensuring that the liquid level height in the vacuum processing cylinder 62 does not exceed the overflow ring plate 63, and ensuring that the thickness of the liquid on the overflow ring plate 63 is not too large.

[0065] The working principle is as follows: the cell sample impact unit 4 impacts the sample to be detected individually into the sheath fluid inside the sheath fluid encapsulation tube 3. The laser detection receiving unit 2 emits a laser, which can pass through the sheath fluid encapsulation tube 3 to detect the cell sample. The detection light signal part of the laser detection receiving unit 2 receives and transmits the reflected light signal. The sheath fluid multi-stage filtration assembly 5 draws the detected sheath fluid into the interior and filters it to separate impurities in the sheath fluid. The sheath fluid vacuum treatment unit 6 evacuates the filtered sheath fluid to eliminate the air bubbles generated in the sheath fluid. Then, the filtered and bubble-removed sheath fluid is refilled into the sheath fluid encapsulation tube 3 to complete the circulation of the sheath fluid.

[0066] The above are all preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made in accordance with the structure, shape and principle of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A flow-type sheath fluid cleaning and circulation and bubble suppression device, characterized in that, include: Control the outer casing (1); Laser detection receiving unit (2), which is installed inside the control outer casing (1), is capable of emitting laser and detecting light signals; Sheath fluid encapsulation tube (3), the sheath fluid encapsulation tube (3) is installed inside the control outer box (1), and the sheath fluid encapsulation tube (3) is installed between the laser detection receiving units (2); Cell sample impact unit (4), the cell sample impact unit (4) is installed inside the sheath fluid wrapping tube (3), the laser of the laser detection receiving unit (2) can pass through the sheath fluid wrapping tube (3) to detect cells in the cell sample impact unit (4), and the cell sample impact unit (4) penetrates the inner wall of the control outer box (1); The sheath fluid multi-stage filtration assembly (5) is installed at one end on the lower side of the sheath fluid wrapping cylinder (3). The sheath fluid multi-stage filtration assembly (5) can extract and filter the sheath fluid inside the sheath fluid wrapping cylinder (3). The sheath fluid multi-stage filtration assembly (5) is installed inside the control outer box (1). It also includes a sheath fluid vacuum treatment unit (6), which is connected to the end of the sheath fluid multi-stage filtration assembly (5) away from the sheath fluid wrapping cylinder (3). The sheath fluid in the sheath fluid multi-stage filtration assembly (5) is filled into the sheath fluid vacuum treatment unit (6). The sheath fluid vacuum treatment unit (6) can vacuum the sheath fluid on the inside. The sheath fluid vacuum treatment unit (6) fills the vacuum-treated sheath fluid back into the sheath fluid wrapping cylinder (3).

2. The flow-type sheath fluid cleaning circulation and bubble suppression device according to claim 1, characterized in that: The sheath fluid multi-stage filtration assembly (5) includes: A collection bottom tube (51) is installed at the lower end of the sheath fluid encapsulation tube (3); A primary power pumping pipe (52) is provided, with its pumping end installed at the bottom of the collecting bottom cylinder (51). The upper end of the filter processing cylinder (53) is connected to the liquid outlet end of the primary power pumping pipe (52). The primary power pumping pipe (52) draws the sheath liquid in the bottom collection cylinder (51) and then fills it into the filter processing cylinder (53). Multiple filter units (54) are installed at equal intervals on the inner side of the filter processing cylinder (53). It also includes an impurity collection component (55), which is installed outside the filter processing cylinder (53) and is capable of collecting impurities filtered by the filter unit (54).

3. The flow-type sheath fluid cleaning circulation and bubble suppression device according to claim 2, characterized in that: The sheath fluid vacuum processing unit (6) includes: A secondary power pumping pipe (61) is provided, with its pumping end installed at the lower end of the collecting bottom cylinder (51). Vacuum processing cylinder (62), the vacuum processing cylinder (62) is installed at the liquid outlet end of the secondary power pumping pipe (61); An overflow ring disk (63) is coaxially mounted on the upper end of the vacuum treatment cylinder (62), and an end cap (64) is provided on the upper side of the overflow ring disk (63). A reflux storage ring (65) is coaxially fixedly sleeved on the outside of the overflow ring (63). The lower end of the end cap (64) is fixed to the upper end of the reflux storage ring (65). A three-stage power pumping pipe (66) is installed on the bottom surface of the reflux storage ring (65). The other end of the three-stage power pumping pipe (66) is connected to the outer surface of the sheath fluid wrapping cylinder (3). It also includes a vacuum tube (67), which is installed through the upper surface of the end cap (64), and the upper end of the vacuum tube (67) penetrates the inner top wall of the control outer casing (1).

4. The flow-type sheath fluid cleaning circulation and bubble suppression device according to claim 2, characterized in that: The filter unit (54) is configured as a cone with a pointed top. The lower end of the filter unit (54) is connected to the filter processing cylinder (53). The outer ring surface of the filter processing cylinder (53) is provided with an impurity discharge groove (56) at the lower end of each filter unit (54). The impurities blocked by the filter unit (54) enter the inner side of the impurity collection assembly (55) through the impurity discharge groove (56).

5. The flow-type sheath fluid cleaning circulation and bubble suppression device according to claim 4, characterized in that: The upper surface of the filter unit (54) is equipped with a plurality of spiral guide plates (57) arranged in a spiral pattern, and the plurality of spiral guide plates (57) are evenly distributed in a circumferential array around the axis of the filter unit (54).

6. The flow-type sheath fluid cleaning circulation and bubble suppression device according to claim 5, characterized in that: The impurity collection component (55) includes: Impurity storage cylinder (551) is fixedly sleeved on the outside of the filter treatment cylinder (53). A discharge pipe (552) is installed at the lower end of the impurity storage cylinder (551), and the other end of the discharge pipe (552) penetrates the inner wall of the control outer box (1). A drive frame (553) is provided between the outside of the filter treatment cylinder (53) and the inside of the impurity storage cylinder (551); It also includes an impurity annular cavity disk (554), the number of which is equal to the number of impurity discharge grooves (56). The impurity annular cavity disk (554) is fixedly sleeved on the outer surface of the impurity storage cylinder (551), the impurity discharge grooves (56) are located inside the impurity annular cavity disk (554), the outer ring surface of the impurity annular cavity disk (554) is open, and an outer sealing cylinder (555) is slidably sleeved on the outer ring surface of the impurity annular cavity disk (554). The outer sealing cylinder (555) is fixed to the drive vertical frame (553), and the drive vertical frame (553) drives the outer sealing cylinder (555) to move up and down.

7. The flow-type sheath fluid cleaning and circulation and bubble suppression device according to claim 6, characterized in that: An inner sealing cylinder (556) is slidably inserted into the inner side of the upper end of the impurity annular cavity disk (554). The inner sealing cylinder (556) is slidably sleeved on the outer surface of the filter treatment cylinder (53). A linkage ring disk (557) is provided on the upper side of the inner sealing cylinder (556). The linkage ring disk (557) is fixed to the drive vertical frame (553). The linkage ring disk (557) is slidably sleeved on the outer surface of the filter treatment cylinder (53). An elastic telescopic rod (558) is fixed on the bottom surface of the linkage ring disk (557). The lower end of the elastic telescopic rod (558) is fixed to the upper surface of the inner sealing cylinder (556).

8. The flow-type sheath fluid cleaning circulation and bubble suppression device according to claim 7, characterized in that: As the inner sealing cylinder (556) and the outer sealing cylinder (555) move downwards following the drive vertical frame (553), the inner sealing cylinder (556) first seals the impurity discharge groove (56), and as the drive vertical frame (553) continues to move downwards, the outer sealing cylinder (555) and the opening of the impurity ring cavity disk (554) are misaligned.

9. The flow-type sheath fluid cleaning and circulation and bubble suppression device according to claim 6, characterized in that: The inner ring surface of the inner bottom wall of the impurity ring cavity disk (554) is located above the outer ring surface of its inner bottom wall. The inner bottom wall of the impurity ring cavity disk (554) is located below the conical surface of the filter unit (54). The lower end of the impurity storage cylinder (551) is an eccentric execution groove. The eccentric opening at the lower end of the impurity storage cylinder (551) is coaxial with the upper end of the impurity discharge pipe (552).

10. The flow-type sheath fluid cleaning circulation and bubble suppression device according to claim 2, characterized in that: The bottom of the collection tube (51) is equipped with an integral liquid replacement pipe (58), and the other end of the integral liquid replacement pipe (58) penetrates the inner wall of the control outer box (1).

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