Leader editing guide RNA, leader editing system and application of leader editing guide RNA and leader editing system

Abstract

The invention discloses a pilot editing guide RNA, a pilot editing system and application of the pilot editing guide RNA and the pilot editing system, and belongs to the technical field related to pilot editing. According to the pilot editing guide RNA provided by the invention, RTT-PBS is placed at a stem-loop2 position of an sgRNA skeleton on the basis of traditional pegRNA, so that the degradation of the RTT-PBS can be effectively avoided, and even if the pegRNA is degraded, the degraded pegRNA does not have a complete sgRNA structure and Cas9 binding ability, so that the efficiency of pilot editing is not influenced.

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