A method and system for identifying and removing redundant otus
By constructing alignment signatures for OTUs and merging redundant OTUs, the problem of OTU redundancy in amplicon sequencing data of the same species was solved, improving the accuracy and efficiency of the analysis.
CN121963897BActive Publication Date: 2026-06-09NANJING NORMAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NANJING NORMAL UNIVERSITY
- Filing Date
- 2026-04-01
- Publication Date
- 2026-06-09
Smart Images
Figure CN121963897B_ABST
Abstract
The application discloses a method and system for identifying and removing redundant OTUs, and relates to biological detection: each sequence OTU_i in a first OTU set is aligned with a species database by using blast to obtain a first matching record set; matching records with a coverage lower than a preset coverage threshold are deleted from the first matching record set to obtain a second matching record set; the matching records in the second matching record set are sorted according to a reference sequence unique identifier to obtain a third matching record set; the matching records in the third matching record set are concatenated in a preset format to obtain an alignment signature of the OTU_i; and the alignment signature of the OTU_i is de-duplicated and merged to obtain a second OTU set; the first OTU set is an OTU set before de-duplication, and the second OTU set is an OTU set after de-duplication, so that the annotation level redundancy problem that cannot be eliminated by a traditional sequence similarity clustering method is effectively solved.
Need to check novelty before this filing date? Find Prior Art