Oil-control antibacterial composition containing ortho-cymene-5-alcohol as well as preparation method and application of oil-control antibacterial composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an o-cymene-5-alcohol-containing oil-control antibacterial composition as well as a preparation method and application of the o-cymene-5-alcohol-containing oil-control antibacterial composition. The oil-control antibacterial composition containing the ortho-cymene-5-ol is prepared from the following components in parts by mass: 5 to 15 parts of the ortho-cymene-5-ol, 1 to 10 parts of a black willow bark extract, 5 to 15 parts of a sake yeast extract, 1 to 10 parts of a camellia flower extract and 1 to 10 parts of an abelmoschus esculentus extract. According to the invention, a composite process of enzymolysis pretreatment and carbon dioxide supercritical extraction is adopted, so that active ingredients can be fully extracted on the premise of protecting the structures of the active ingredients from being damaged, and the antibacterial effect is remarkably enhanced; all the components are scientifically compounded, synergistic interaction of the oil control effect is achieved, through the synergistic effect of the components, the oil control rate is increased, the oil control effect is more durable, and the problems of greasy skin, floodlight and the like can be effectively solved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an oil-controlling and antibacterial composition containing o-cymene-5-ol, its preparation method, and its application. Background Technology

[0002] With the fast pace of life, changes in dietary structure, and environmental factors, oily skin problems are becoming increasingly common. This manifests not only as excessive sebum secretion and a shiny, greasy appearance, but also as a tendency to clogged pores, acne, folliculitis, and other skin issues. The core cause of this phenomenon lies in the overactive sebaceous glands leading to excessive sebum accumulation, coupled with an imbalance in the skin's microecology, resulting in the proliferation of pathogenic bacteria such as Propionibacterium acnes and Staphylococcus aureus. These two factors interact and exacerbate skin problems. Therefore, developing compositions that combine excellent oil-control effects with highly effective antibacterial properties has become a research hotspot in the cosmetics and skincare industry.

[0003] Currently, most skincare products for oily skin focus on a single function of "oil control" or "antibacterial action," or attempt to combine both. However, they generally suffer from the technical challenge of not being able to simultaneously achieve excellent oil control and antibacterial effects. The core ingredients in existing oil-control products are mostly adsorbents or oil-regulating agents. Adsorbents can only physically adsorb existing sebum on the skin's surface, failing to inhibit sebum secretion at its source. Their oil-control effect is short-lived, and they have no inhibitory effect on pathogenic bacteria on the skin's surface, failing to address the bacterial proliferation caused by sebum buildup. While oil-regulating agents can inhibit sebaceous gland activity to some extent, their antibacterial ability is weak. When the number of pathogenic bacteria on the skin's surface is high, it is still difficult to prevent inflammatory reactions such as acne. Existing antibacterial products mostly use chemical bactericides or single plant extracts. While chemical bactericides have significant antibacterial effects, they are also highly irritating. Long-term use can damage the skin barrier and even lead to further imbalance in the skin's microecology, exacerbating sebum secretion disorders. Single plant extracts have limited antibacterial activity and lack targeted oil-controlling ingredients, making it difficult to solve the core problem of excessive sebum secretion and achieve a synergistic improvement effect of "oil control + antibacterial".

[0004] To overcome the shortcomings of single efficacy, some existing technologies attempt to combine oil-controlling and antibacterial ingredients. However, due to improper ingredient selection, imbalanced ratios, or outdated preparation processes for key ingredients, the compound system cannot achieve synergistic effects and still cannot simultaneously achieve excellent oil-controlling and antibacterial effects. Specific defects include: (1) Poor ingredient compatibility: Some compound products blindly mix oil-controlling and antibacterial ingredients without considering the interaction between ingredients. Although some plant extracts have certain potential for both oil control and antibacterial effects, the extraction process has not been optimized in existing technologies, resulting in low content and poor purity of active ingredients in the extracts, which cannot fully exert their effects. (2) Imbalanced compound ratios: The ingredient ratios of existing compound products are mostly empirical ratios and have not been systematically optimized. Either the oil-controlling ingredients are excessive and the antibacterial ingredients are insufficient, resulting in temporary oil control but no inhibition of bacterial growth, which easily leads to repeated skin inflammation; or the antibacterial ingredients are excessive and the oil-controlling ingredients are insufficient, resulting in the inhibition of bacteria but no solution to the oil secretion problem, and the skin is still in an oily state.

[0005] Therefore, developing a composition with good ingredient compatibility, scientific ratio, and high activity of key components that can simultaneously achieve efficient oil control and strong antibacterial effect has significant practical significance and market value. Summary of the Invention

[0006] The purpose of this invention is to provide an oil-controlling and antibacterial composition containing o-cymene-5-ol, its preparation method, and its application.

[0007] To achieve the above objectives, the present invention provides the following technical solution: An oil-controlling and antibacterial composition containing o-cymene-5-ol comprises the following components in parts by weight: 5-15 parts o-cymene-5-ol, 1-10 parts black willow bark extract, 5-15 parts sake yeast extract, 1-10 parts camellia extract, and 1-10 parts okra extract.

[0008] The preparation method of the black willow bark extract includes the following steps: (1) The black willow bark was washed and dried in an oven at 50°C until constant weight. Then it was pulverized to less than 60 mesh using a high-speed pulverizer to obtain black willow bark powder. (2) Mix black willow bark powder with deionized water at a ratio of 1:20 (g / mL), stir evenly, adjust the pH of the system to 4.5-5.5, add 0.8%-1.2% of the mass of black willow bark powder of compound enzyme, which is composed of cellulase and hemicellulase mixed at a mass ratio of 2:1, and enzymatically hydrolyze in a constant temperature water bath at 45-50℃ for 2.5-3.5h. After the enzymatic hydrolysis is completed, raise the temperature to 90-95℃ and keep it warm for 15-20min to obtain the enzymatic hydrolysate. (3) Place the enzymatic hydrolysate in a rotary evaporator and concentrate it to 1 / 5-1 / 4 of the original volume under a vacuum of 0.08 MPa and a temperature of 50-60℃ to obtain a concentrated solution; (4) Place the concentrate in a supercritical extraction vessel, add 5%-8% ethanol by mass of the concentrate as an entrainer, set the extraction pressure to 30-35 MPa, the extraction temperature to 40-45℃, the CO2 flow rate to 20-25 L / h, and the extraction time to 2-3 h; after extraction, separate CO2 and extract through a vacuum separation vessel at a separation pressure of 5-8 MPa and a separation temperature of 30-35℃ to obtain crude extract; filter the crude extract through a 0.22 μm filter membrane; (5) Place the filtered extract in a freeze dryer and freeze dry it to constant weight under vacuum of 10 Pa and temperature of -40 °C. Then pulverize it to less than 100 mesh to obtain black willow bark extract.

[0009] This invention overcomes the limitations of traditional single-process extraction of black willow bark extract by employing a combined process of enzymatic pretreatment and supercritical carbon dioxide extraction. This process fully extracts the active ingredients while protecting their structure. Compared to traditional extraction methods, the black willow bark extract prepared by this method has a higher content of antibacterial active ingredients, a larger inhibition zone diameter against common skin pathogens such as Propionibacterium acnes and Staphylococcus aureus, and a significantly enhanced antibacterial effect.

[0010] An oil-controlling and antibacterial composition containing o-cymene-5-ol comprises the following components in parts by weight: 8-12 parts o-cymene-5-ol, 5-8 parts black willow bark extract, 6-10 parts sake yeast extract, 4-7 parts camellia extract, and 3-6 parts okra extract.

[0011] The components of this invention are scientifically compounded to achieve a synergistic effect in oil control. o-Cymenocyanate-5-ol not only has a strong antibacterial effect but also inhibits excessive secretion from sebaceous gland cells; black willow bark extract can regulate skin keratin metabolism, unclog pores, and reduce oil accumulation; sake yeast extract can regulate the skin's microecological balance, enhance the skin barrier function, and reduce sebum secretion disorders caused by microecological imbalance; the flavonoids and polysaccharides in camellia flower extract and okra extract have soothing and anti-inflammatory effects, relieving skin inflammation caused by excessive sebum secretion while further inhibiting sebaceous gland secretion. Through the synergistic effect of the above components, this composition can control oil from multiple dimensions, including inhibiting secretion, unclogging pores, regulating the microecology, and soothing and anti-inflammatory effects, improving the oil control rate and making the oil control effect more lasting, effectively improving problems such as oily skin and shine.

[0012] A method for preparing an oil-controlling and antibacterial composition containing o-cymene-5-ol includes the following steps: mixing o-cymene-5-ol, black willow bark extract, sake yeast extract, camellia extract, and coffee sage extract evenly to obtain an oil-controlling and antibacterial composition containing o-cymene-5-ol.

[0013] Application of an oil-controlling and antibacterial composition containing o-cymene-5-ol in the preparation of cosmetics.

[0014] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows: 1. The present invention uses a combined process of enzymatic pretreatment and supercritical carbon dioxide extraction, which can fully extract the active ingredients while protecting the structure of the active ingredients. This increases the diameter of the inhibition zone against common skin pathogens such as Propionibacterium acnes and Staphylococcus aureus, and significantly enhances the antibacterial effect.

[0015] 2. The components of this invention are scientifically compounded to achieve a synergistic effect in oil control. Through the synergistic effect of the components, this composition can control oil from multiple dimensions such as inhibiting secretion, unclogging pores, regulating microecology, and soothing and anti-inflammatory. The oil control rate is improved and the oil control effect is longer-lasting, which can effectively improve problems such as oily skin and shine. Detailed Implementation

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] Example 1 This embodiment provides an oil-controlling and antibacterial composition containing o-cymene-5-ol, comprising the following components in parts by weight: 10 parts o-cymene-5-ol, 7 parts black willow bark extract, 8 parts sake yeast extract, 5 parts camellia extract, and 4 parts okra extract.

[0018] The preparation method of the black willow bark extract includes the following steps: (1) The black willow bark was washed and dried in an oven at 50°C until constant weight. Then it was pulverized to less than 60 mesh using a high-speed pulverizer to obtain black willow bark powder. (2) Mix black willow bark powder with deionized water at a ratio of 1:20 (g / mL), stir evenly, adjust the pH of the system to 5.0, add 1.0% of the mass of black willow bark powder of compound enzyme, which is composed of cellulase and hemicellulase mixed at a mass ratio of 2:1, and hydrolyze in a constant temperature water bath at 50℃ for 3 hours. After the hydrolysis is completed, raise the temperature to 95℃ and keep it warm for 20 minutes to obtain the hydrolysate. (3) The enzymatic hydrolysate was placed in a rotary evaporator and concentrated to 1 / 4 of its original volume under a vacuum of 0.08 MPa and a temperature of 60 °C to obtain a concentrated solution; (4) Place the concentrate in a supercritical extraction vessel, add 6% ethanol by mass of the concentrate as an entrainer, set the extraction pressure to 35 MPa, the extraction temperature to 40 °C, the CO2 flow rate to 25 L / h, and the extraction time to 3 h; after extraction, separate CO2 and extract through a vacuum separation vessel at a separation pressure of 8 MPa and a separation temperature of 30 °C to obtain crude extract; filter the crude extract through a 0.22 μm filter membrane; (5) Place the filtered extract in a freeze dryer and freeze dry it to constant weight under vacuum of 10 Pa and temperature of -40 °C. Then pulverize it to less than 100 mesh to obtain black willow bark extract.

[0019] A method for preparing an oil-controlling and antibacterial composition containing o-cymene-5-ol includes the following steps: mixing o-cymene-5-ol, black willow bark extract, sake yeast extract, camellia extract, and coffee sage extract evenly to obtain an oil-controlling and antibacterial composition containing o-cymene-5-ol.

[0020] Example 2 This embodiment provides an oil-controlling and antibacterial composition containing o-cymene-5-ol, comprising the following components in parts by weight: 8 parts o-cymene-5-ol, 8 parts black willow bark extract, 6 parts sake yeast extract, 7 parts camellia extract, and 3 parts coffee okra extract.

[0021] The preparation method of the black willow bark extract includes the following steps: (1) The black willow bark was washed and dried in an oven at 50°C until constant weight. Then it was pulverized to less than 60 mesh using a high-speed pulverizer to obtain black willow bark powder. (2) Mix black willow bark powder with deionized water at a ratio of 1:20 (g / mL), stir evenly, adjust the pH of the system to 5.0, add 1.0% of the mass of black willow bark powder of compound enzyme, which is composed of cellulase and hemicellulase mixed at a mass ratio of 2:1, and hydrolyze in a constant temperature water bath at 50℃ for 3 hours. After the hydrolysis is completed, raise the temperature to 95℃ and keep it warm for 20 minutes to obtain the hydrolysate. (3) The enzymatic hydrolysate was placed in a rotary evaporator and concentrated to 1 / 4 of its original volume under a vacuum of 0.08 MPa and a temperature of 60 °C to obtain a concentrated solution; (4) Place the concentrate in a supercritical extraction vessel, add 6% ethanol by mass of the concentrate as an entrainer, set the extraction pressure to 35 MPa, the extraction temperature to 40 °C, the CO2 flow rate to 25 L / h, and the extraction time to 3 h; after extraction, separate CO2 and extract through a vacuum separation vessel at a separation pressure of 8 MPa and a separation temperature of 30 °C to obtain crude extract; filter the crude extract through a 0.22 μm filter membrane; (5) Place the filtered extract in a freeze dryer and freeze dry it to constant weight under vacuum of 10 Pa and temperature of -40 °C. Then pulverize it to less than 100 mesh to obtain black willow bark extract.

[0022] A method for preparing an oil-controlling and antibacterial composition containing o-cymene-5-ol includes the following steps: mixing o-cymene-5-ol, black willow bark extract, sake yeast extract, camellia extract, and coffee sage extract evenly to obtain an oil-controlling and antibacterial composition containing o-cymene-5-ol.

[0023] Comparative Example 1 The difference between this comparative example and Example 1 is that the black willow bark extract is a commercially available product from Baoji Fang Sheng Biotechnology Development Co., Ltd.

[0024] Comparative Example 2 The difference between this comparative example and Example 1 is that the complex enzyme is composed of cellulase and hemicellulase mixed in a mass ratio of 1:2.

[0025] Comparative Example 3 The difference between this comparative example and Example 1 is that the black willow bark extract was prepared using a traditional solvent extraction method.

[0026] The steps for preparing black willow bark extract using the traditional ethanol reflux extraction method are as follows: (1) After washing the black willow bark, dry it in an oven at 50°C until constant weight, then pulverize it to less than 60 mesh to obtain black willow bark powder; (2) Mix black willow bark powder with 75% ethanol at a material-to-liquid ratio of 1:20 (g / mL), place in a reflux reflux device, and extract at 80℃ for 3 hours. Repeat the extraction twice. (3) Combine the two extracts, filter them and place them in a rotary evaporator. Concentrate them under reduced pressure at 60°C until there is no ethanol odor. Then freeze-dry and pulverize them to less than 100 mesh to obtain black willow bark extract.

[0027] Comparative Example 4 The difference between this comparative example and Example 1 is that the preparation method of the black willow bark extract includes the following steps: (1) The black willow bark was washed and dried in an oven at 50°C until constant weight. Then it was pulverized to less than 60 mesh using a high-speed pulverizer to obtain black willow bark powder. (2) Mix black willow bark powder with deionized water at a ratio of 1:5 (g / mL) to obtain a mixture. Place the mixture in a supercritical extraction vessel and add 6% ethanol by mass of the mixture as an entrainer. Set the extraction pressure to 35 MPa, the extraction temperature to 40 °C, the CO2 flow rate to 25 L / h, and the extraction time to 3 h. After extraction, separate CO2 and extract through a vacuum separation vessel at a pressure of 8 MPa and a separation temperature of 30 °C to obtain a crude extract. Filter the crude extract through a 0.22 μm filter membrane. (3) The filtered extract was placed in a freeze dryer and freeze-dried to constant weight under vacuum of 10 Pa and temperature of -40 °C. The extract was then pulverized to less than 100 mesh to obtain black willow bark extract.

[0028] Comparative Example 5 The difference between this comparative example and Example 1 is that the camellia extract was replaced with astragalus extract.

[0029] Comparative Example 6 The difference between this comparative example and Example 1 is that: an oil-controlling and antibacterial composition containing o-cymene-5-ol comprises the following components in parts by weight: 14 parts o-cymene-5-ol, 3 parts black willow bark extract, 12 parts sake yeast extract, 2 parts camellia extract, and 8 parts coffee okra extract.

[0030] Performance testing The oil-controlling and antibacterial compositions prepared in Examples 1-2 and Comparative Examples 1-6 were subjected to antibacterial and oil-controlling tests.

[0031] 1. Antibacterial test: Test strains: Propionibacterium acnes (ATCC6919) and Staphylococcus aureus (ATCC25923), both purchased from the China General Microbiological Culture Collection Center.

[0032] Test method: The agar diffusion method (GB / T21510-2008) was used. The diameter of the inhibition zone (mm) was measured with vernier calipers. Three parallel experiments were set up for each sample, and the average value was taken. The larger the diameter of the inhibition zone, the stronger the antibacterial effect.

[0033] 2. Oil control test: Test model: The test model was a testosterone-induced mouse sebaceous gland hyperplasia model (simulating the state of excessive sebum secretion in oily skin). The test subjects were SPF-grade Kunming mice, half male and half female, weighing 20-22g. The housing environment was 25℃, relative humidity 55%, circadian rhythm 12h / 12h, and free access to food and water.

[0034] Test Method: The skin oil gravimetric method (ether extraction method) was used. The specific steps are as follows: After acclimatization for 3 days, all mice except the blank control group were intraperitoneally injected with testosterone solution (concentration 5mg / mL, solvent is physiological saline + 5% ethanol), at a dose of 0.1mL / 10g body weight, once a day for 7 consecutive days to establish a sebaceous gland hyperplasia model; the blank control group was intraperitoneally injected with an equal volume of physiological saline + 5% ethanol solution; the successfully modeled mice were randomly divided into 8 groups of 6 mice each. The compositions of the examples and comparative examples were prepared into a 10% (w / w) sample solution with deionized water. The sample solution was evenly applied to the hair removal area on the back of the mice (hair removal area 2cm×2cm, treated with 8% sodium sulfide solution before hair removal, and cleaned with physiological saline after hair removal) twice a day, morning and evening, at a rate of 0.1mL / mouse, for 7 consecutive days. Twenty-four hours after the last application, mice were euthanized by cervical dislocation, and the skin tissue from the hairless area on the back was immediately cut off and accurately weighed (recorded as W1). The skin tissue was placed in a stoppered centrifuge tube, 5 mL of anhydrous ether was added, and the tube was sealed and placed in a shaker. Extraction was carried out at 25°C and 150 r / min for 1 hour, followed by centrifugation at 3000 r / min for 5 minutes. The supernatant was transferred to a pre-weighed evaporating dish (recorded as W0). 3 mL of anhydrous ether was added to the centrifuge tube for a second extraction, and the supernatants from both extractions were combined. The evaporating dish containing the supernatant was placed in a fume hood and dried in a 60°C oven for 30 minutes after the ether had completely evaporated. After drying, it was placed in a desiccator to cool to room temperature and accurately weighed (recorded as W2). The skin oil content and oil control rate were calculated using the following formula: Skin oil content (mg / g) = (W2 - W0) / W1 × 1000.

[0035] The test results are shown in Table 1.

[0036] Table 1 Performance Test Results

[0037] The results show that the oil-controlling and antibacterial compositions of Examples 1-2 have excellent oil-controlling and antibacterial effects.

[0038] Comparative Example 1 used commercially available black willow bark extract. Due to the insufficient content of antibacterial and oil-controlling active ingredients in the commercially available extract, it could not form an effective synergy with components such as o-cymene-5-ol and sake yeast extract, resulting in a significant decrease in the overall oil-controlling and antibacterial effect.

[0039] In Comparative Example 2, the cellulase content was insufficient, which could not effectively break down the cellulose skeleton, resulting in a significant decrease in the effect of enzymatic hydrolysis pretreatment and making it difficult to fully release the active ingredients in the black willow bark.

[0040] Comparative Example 3 used the traditional ethanol reflux extraction method to prepare black willow bark extract, which led to the degradation of the heat-sensitive antibacterial active ingredients in the black willow bark, reducing its biological activity; the extraction process lacked enzymatic pretreatment, the cell wall structure remained intact, and the active ingredients were difficult to fully dissolve. Comparative Example 4 lacked the enzymatic hydrolysis step, making it difficult for the active ingredients to be released from the dense cell wall even when using supercritical extraction, resulting in a significant reduction in extraction efficiency.

[0041] Comparative Example 5 replaced camellia extract with an equal mass of astragalus extract, which disrupted the scientific compounding system of this invention. Camellia extract contains active ingredients such as flavonoids and polysaccharides, which can soothe skin inflammation and regulate the secretion rhythm of sebaceous glands. It forms a highly efficient synergy with the antibacterial effects of o-cymene-5-ol, the synergistic antibacterial effects of black willow bark extract, and the microecological regulation effects of sake yeast extract, thus jointly enhancing the oil control and antibacterial effects. On the other hand, the core components of astragalus extract are astragalus polysaccharides and astragalus saponins, whose main function is to enhance immunity. They lack the effects of soothing and anti-inflammatory and assisting in oil control, and cannot replace camellia extract to form a synergistic effect with other components.

[0042] The change in the component mass fraction of Comparative Example 6 led to an imbalance in the synergistic effect of the components. The excessive amount of certain components caused an imbalance in the skin microecology, which weakened the overall oil control and antibacterial effect.

[0043] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An oil-controlling and antibacterial composition containing o-cymene-5-ol, characterized in that, The product contains the following components in parts by weight: 5-15 parts of o-cymene-5-ol, 1-10 parts of black willow bark extract, 5-15 parts of sake yeast extract, 1-10 parts of camellia flower extract, and 1-10 parts of okra extract.

2. The oil-controlling and antibacterial composition containing o-cymene-5-ol according to claim 1, characterized in that, The product contains the following components in parts by weight: 8-12 parts of o-cymene-5-ol, 5-8 parts of black willow bark extract, 6-10 parts of sake yeast extract, 4-7 parts of camellia flower extract, and 3-6 parts of okra extract.

3. The oil-controlling and antibacterial composition containing o-cymene-5-ol according to claim 2, characterized in that, The preparation method of the black willow bark extract includes the following steps: (1) Wash the black willow bark, dry it to constant weight, and then crush it to obtain black willow bark powder; (2) Mix black willow bark powder with deionized water, stir evenly, adjust the pH of the system to 4.5-5.5, add compound enzyme, and hydrolyze at a constant temperature of 45-50℃ for 2.5-3.5h. After the hydrolysis is completed, inactivate the enzyme to obtain the hydrolysate. (3) Place the enzymatic hydrolysate in a rotary evaporator and concentrate it to 1 / 5-1 / 4 of its original volume to obtain a concentrated solution; (4) Place the concentrate in a supercritical extraction vessel, add ethanol as an entrainer, and extract; after extraction, separate CO2 and extract through a vacuum separation vessel to obtain crude extract; filter the crude extract to obtain the filtered extract. (5) Freeze-dry the filtered extract to constant weight, pulverize, and obtain black willow bark extract.

4. The oil-controlling and antibacterial composition containing o-cymene-5-ol according to claim 3, characterized in that, The complex enzyme is a mixture of cellulase and hemicellulase.

5. The oil-controlling and antibacterial composition containing o-cymene-5-ol according to claim 4, characterized in that, The complex enzyme is composed of cellulase and hemicellulase mixed in a mass ratio of (1.8-2.2):

1.

6. The oil-controlling and antibacterial composition containing o-cymene-5-ol according to claim 3, characterized in that, The supercritical extraction vessel is set with an extraction pressure of 30-35 MPa, an extraction temperature of 40-45℃, a CO2 flow rate of 20-25 L / h, and an extraction time of 2-3 h.

7. The oil-controlling and antibacterial composition containing o-cymene-5-ol according to claim 3, characterized in that, Separation pressure 5-8MPa, separation temperature 30-35℃.

8. The oil-controlling and antibacterial composition containing o-cymene-5-ol according to claim 3, characterized in that, Add 0.8%-1.2% (by weight) of a compound enzyme to black willow bark powder.

9. A method for preparing an oil-controlling and antibacterial composition containing o-cymene-5-ol according to any one of claims 1-8, characterized in that, The process includes the following steps: mixing o-cymene-5-ol, black willow bark extract, sake yeast extract, camellia extract, and coffee sage extract evenly to obtain an oil-controlling and antibacterial composition containing o-cymene-5-ol.

10. The use of the oil-controlling and antibacterial composition containing o-cymene-5-ol according to any one of claims 1-8 in the preparation of cosmetics.

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