Organ-like construction method and application thereof
By adjusting the chain length of helical polyisocyanates and RGD modification, combined with click chemistry, the problem of difficulty in controlling the differentiation pathways of cartilage and bone organoids in existing technologies has been solved, achieving efficient construction and functional optimization of organoids, which is applicable to tissue engineering and regenerative medicine research.
Patent Information
- Application Number
- CN202411583606.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-07
- Publication Date
- 2026-05-08
AI Technical Summary
Existing technologies make it difficult to precisely control the differentiation pathways of cartilage and bone organoids in a three-dimensional culture system, resulting in limitations in the functionality and tissue maturity of these organoids, which restricts their performance in simulating real tissue functions and achieving regenerative capabilities.
Using helical polyisocyanates as the extracellular matrix, differentiation of chondrogenic or osteoblastic cell populations is achieved by adjusting chain length and RGD modification. The click chemistry of azide-modified polyisocyanates allows for rapid binding with specific molecules, optimizing cell-matrix interactions and enabling effective regulation of organoid differentiation pathways.
It enables precise control of the differentiation pathways of cartilage and bone organoids, improves the functionality and tissue maturity of organoids, adapts to the needs of different research and clinical applications, provides a more ideal biomimetic microenvironment, and is suitable for a wide range of tissue engineering applications.