Whitening and freckle-removing skin care product composition and preparation method thereof

Abstract

The invention relates to a whitening and freckle-removing skin care product composition and a preparation method thereof, and belongs to the field of cosmetics. The composition is prepared from the active ingredients such as the bifid yeast fermentation product filtrate, the glycyrrhiza glabra extract and the curcuma zedoaria root extract, and the active ingredients are matched with one another, so that the composition is endowed with excellent effects of whitening, removing freckles, preserving moisture, resisting inflammation and the like.

Description

Technical Field

[0001] This invention relates to a whitening and freckle-removing skincare composition and its preparation method, belonging to the field of cosmetics. Background Technology

[0002] Whitening and spot-removing skincare product compositions typically work through multiple whitening pathways. They can inhibit tyrosinase activity, reducing melanin production at its source, and accelerate melanin metabolism, reducing existing melanin. Simultaneously, some ingredients can also suppress inflammatory responses and repair damaged skin barriers, achieving whitening and spot-removing effects from multiple dimensions and addressing pigmentation problems caused by inflammation and skin barrier issues.

[0003] Invention patent CN119732882B discloses a whitening and freckle-removing active composition, its preparation method and its application in cosmetics. The whitening and freckle-removing active composition includes Rhodiola crenulata root extract, Morus alba extract and hexapeptide-2.

[0004] Invention patent CN116440043B discloses a whitening and freckle-removing cream, which contains the following components by weight: water, glycerin, mineral oil, antioxidant microcapsules, polydimethylsiloxane, sodium ascorbate phosphate, niacinamide, plant compound extract, fragrance, and cetearyl alcohol.

[0005] Invention patent CN119097567B discloses a whitening and freckle-removing essence and its preparation method. The whitening and freckle-removing essence is formed by mixing additives such as niacinamide, EDTA, L-lactic acid, citric acid, and baicalin encapsulation.

[0006] Invention patent CN118924667B discloses a plant composition with whitening and freckle-removing effects and its application. The plant composition includes the following raw materials: sea fennel extract, osmanthus extract, barley extract, smooth elm bark extract, black sesame extract, thyme extract, spinach extract and solvent.

[0007] Researching a technology that utilizes multiple pathways to achieve excellent skin whitening and freckle removal effects remains one of the technical problems that need to be solved. Summary of the Invention

[0008] In response to the aforementioned technical problems, the inventors have provided relevant research content for this invention, specifically including a whitening and freckle-removing skincare composition and its preparation method, which belongs to the field of cosmetics.

[0009] The present invention provides a skin whitening and freckle-removing skin care product composition, wherein the raw materials are composed of: 0.05-0.5 parts of Bifida Ferment Lysate Ferment Filtrate, 0.1-1.5 parts of Glycyrrhiza Glabra Extract, and 0.1-1.5 parts of Spatholobus Suberectus Root Extract by Weight.

[0010] Furthermore, the whitening and freckle-removing skin care product composition, by weight, also contains: 2-4 parts diglyceride, 8-15 parts glycerin, 2-6 parts polydimethylsiloxane, 0.1-1.5 parts sodium hyaluronate, 0.05-0.5 parts ascorbate glucoside, 0.02-0.3 parts ceramide NP and 60-90 parts water.

[0011] Furthermore, the whitening and freckle-removing skin care composition, by weight, also contains: 0.1-1.5 parts of *Saussurea involucrata* extract, 0.1-1.5 parts of *Echinacea purpurea* extract, and 2-4.5 parts of emulsifier.

[0012] Furthermore, the weight ratio of licorice root extract to Saururus chinensis extract is 1:0.6-1.3.

[0013] Furthermore, the emulsifier includes at least one of Tween-60, Tween-80, Tween-20, cetearyl alcohol polyether-20, and cocamidopropyl betaine.

[0014] Furthermore, the preparation method of licorice root extract includes: taking licorice root, crushing it, then mixing it with an ethanol solution with a concentration of 20-35wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract.

[0015] Furthermore, the preparation method of the root extract of *Symplocos buergeriana* includes: crushing the root of *Symplocos buergeriana*, mixing it with an ethanol solution of 50-65 wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract.

[0016] Furthermore, the preparation method of the *Saururus chinensis* extract includes: crushing the *Saururus chinensis*, mixing it with an ethanol solution of 20-35 wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract.

[0017] Furthermore, the preparation method of purple coneflower extract includes: taking purple coneflower, crushing it, then mixing it with an ethanol solution with a concentration of 50-65wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract.

[0018] Furthermore, the weight ratio of the extracts of *Symplocos buergeriana* root and *Echinacea purpurea* was 0.2-1:1.

[0019] The present invention also provides a method for preparing the whitening and freckle-removing skin care product composition, wherein the preparation method includes stirring and mixing the raw materials and sterilizing them.

[0020] The present invention also provides the application of the whitening and freckle-removing skin care composition in the preparation of whitening or freckle-removing cosmetics.

[0021] The beneficial effects of this invention are:

[0022] This invention uses Bifida ferment filtrate, licorice root extract, and Sophora flavescens root extract to prepare a composition. The above active ingredients work together to give the composition excellent whitening, spot-removing, moisturizing and anti-inflammatory effects.

[0023] The extracts of *Polygonum aviculare* root and *Echinacea purpurea* prepared in this invention both exhibit excellent anti-inflammatory effects, effectively inhibiting the expression of inflammatory factors. The extracts of *Polygonum aviculare* root and *Echinacea purpurea* show a synergistic effect in a weight ratio ranging from 0.12:1 to 6:1, enhancing the anti-inflammatory effect. When the two are combined in a weight ratio ranging from 0.2:1 to 1:1, the anti-inflammatory effect is significantly enhanced, effectively inhibiting inflammation.

[0024] The use of licorice root extract and Saururus chinensis extract in the composition of this invention can effectively enhance the whitening and spot-removing effects of the composition. When licorice root extract and Saururus chinensis extract are used simultaneously in the composition of this invention, they can be combined to enhance the whitening and spot-removing effects, and synergistically improve the richness and variety of whitening active ingredients, giving the product excellent results.

[0025] The preparation process of this invention is simple and the product has excellent efficacy, which has good market prospects. Attached Figure Description

[0026] Figure 1 : Expression level of TN-α;

[0027] Figure 2 IL-6 expression level;

[0028] Figure 3 ζ: Efficiency of inhibiting melanin formation. Detailed Implementation

[0029] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification. Many specific details are set forth in the following description to provide a thorough understanding of the present invention; however, the present invention may also be implemented in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0030] Glycyrrhiza glabra extract: The whole plant of Glycyrrhiza glabra was dried to a water content of 1.64% by weight, pulverized, and then the pulverized material was mixed with a 30wt% ethanol solution at a weight ratio of 1:15. The mixture was heated to 55℃ and stirred at 100 rpm for 4.2 hours. The filtrate was then obtained by filtration, concentrated at 60℃ and 0.005 MPa to a filtrate weight of 20.3%, and freeze-dried at -60℃ to a water content of 1.33% by weight.

[0031] Cyanotis arachnoidea root extract: The roots of Cyanotis arachnoidea were dried to a water content of 1.53 wt%, pulverized, and then the pulverized material was mixed with a 60 wt% ethanol solution at a weight ratio of 1:20. The mixture was heated to 50°C and stirred at 100 rpm for 5.5 hours. The extract was then filtered to obtain the filtrate, which was then concentrated at 60°C and 0.006 MPa to 21.5% of the filtrate weight. Finally, it was freeze-dried at -60°C to a water content of 1.42% to obtain the final product.

[0032] Saururus Chinesesis Extract: The whole plant of Saururus Chinesesis was dried to a water content of 1.95 wt%, pulverized, and then the pulverized material was mixed with a 28 wt% ethanol solution at a weight ratio of 1:25. The mixture was heated to 60℃ and stirred at 100 rpm for 4 hours. The filtrate was then obtained by filtration, concentrated at 60℃ and 0.008 MPa to 21.2% of the filtrate weight, and freeze-dried at -60℃ to a water content of 1.25%.

[0033] Purple coneflower (Echinacea purpurea) extract: The whole plant of purple coneflower was dried to a water content of 2.16 wt%, pulverized, and then the pulverized material was mixed with a 62.5 wt% ethanol solution at a weight ratio of 1:28. The mixture was heated to 54℃ and stirred at 100 rpm for 5 hours. The filtrate was then obtained by filtration, concentrated at 60℃ and 0.006 MPa to 19.8% of the filtrate weight, and freeze-dried at -60℃ to a water content of 1.78%.

[0034] Bifida Ferment Lysate Ferment Filtrate: Ferment-DFF, Guangdong Dimei New Material Technology Co., Ltd. Sodium Hyaluronate: Product No. S31737, Shanghai Yuanye Biotechnology Co., Ltd.

[0035] Example 1

[0036] Prepare the samples for testing, measured by weight. Samples 1-8 are compound products composed of extracts of *Symplocos buergeriana* root and *Echinacea purpurea*, with corresponding weight ratios of 0.08:1, 0.12:1, 0.2:1, 0.5:1, 0.8:1, 1:1, 3:1, and 6:1, respectively. Samples 9-10 are single products of *Symplocos buergeriana* root and *Echinacea purpurea* extract, respectively. The anti-inflammatory effects of the samples will be tested.

[0037] RAW264.7 cells were added to 24-well plates until the cell density reached 1.2 × 10⁻⁶ cells / well. 5 DMEM medium was added to each well to a final volume of 0.5 mL, and the mixture was incubated for 24 hours. The old medium was then removed, and the samples were divided into 12 groups (blank group, model group, and test sample groups 1-10, with 4 replicates per group). Fresh DMEM medium was added to the blank group to a final volume of 0.5 mL, fresh DMEM medium (containing 1 μg / mL LPS) to the model group to a final volume of 0.5 mL, and fresh DMEM medium (containing 1 μg / mL LPS + 50 μg / mL test sample) to the test sample groups to a final volume of 0.5 mL. All groups were incubated for another 24 hours. The supernatant was collected, and the expression levels of IL-6 and TN-α were detected according to the ELISA kit instructions. The results are shown in Table 1 and [Table data would be inserted here]. Figure 1-2 .

[0038] Table 1: Anti-inflammatory test Serial Number TNF-α / pg / mL IL-6 / pg / mL Blank group 47.87 20.54 Model group 145.79 113.40 Sample 1 to be tested 74.60 52.69 Sample 2 to be tested 72.28 50.48 Sample 3 to be tested 64.51 43.11 Sample 4 to be tested 58.73 37.62 Sample 5 to be tested 56.42 35.53 Sample 6 to be tested 63.29 41.95 Sample 7 to be tested 70.87 49.18 Sample 8 to be tested 72.65 50.85 Sample 9 to be tested 72.74 51.97 10 samples to be tested 75.61 53.64

[0039] As shown in Table 1 above, the extracts of *Polygonum aviculare* root and *Echinacea purpurea* prepared in this invention both exhibit excellent anti-inflammatory effects and effectively inhibit the expression of inflammatory factors. The extracts of *Polygonum aviculare* root and *Echinacea purpurea* show a synergistic effect within a weight ratio range of 0.12:1 to 6:1, enhancing the anti-inflammatory effect. When the two are combined in a weight ratio range of 0.2:1 to 1:1, the anti-inflammatory effect is significantly enhanced, effectively inhibiting inflammation.

[0040] Example 2

[0041] The composition was prepared according to the raw materials and dosages in Table 2.

[0042] Table 2: Raw Material Composition Composition No. / Part 1 2 3 4 5 Bifida ferment filtrate 0.08 0.08 0.08 0.07 0.08 Licorice extract 0 2.4 0 0.9 1.2 Blue ear grass root extract 1.2 1.2 1.2 1 1.2 diglycerides 3 3 3 3.2 3 glycerin 10 10 10 10.5 10 polydimethylsiloxane 5 5 5 4.8 5 Sodium hyaluronate 0.2 0.2 0.2 0.15 0.2 Ascorbate glucoside 0.1 0.1 0.1 0.12 0.1 Ceramide NP 0.07 0.07 0.07 0.08 0.07 Three White Herbs Extract 0 0 2.4 1.5 1.2 Purple coneflower extract 1.2 1.2 1.2 1.4 1.2 Twain-60 2 2 2 0 2 Twain-20 2.2 2.2 2.2 4.3 2.2 water Up to 100 copies Up to 100 copies Up to 100 copies Up to 100 copies Up to 100 copies

[0043] The preparation method of the above composition is as follows:

[0044] (1) Weigh the raw materials according to the weight; add water to reactor 1, heat to 50°C, add emulsifier (Tween-20, Tween-60), and stir at 60 rpm for 10 minutes;

[0045] (2) Add glycerol and diglycerol to reaction vessel 2, heat to 60°C, add polydimethylsiloxane, and stir at 80 rpm for 20 minutes;

[0046] (3) Add the material from reactor 2 to reactor 1, keep it at 50°C and stir at 200 rpm for 30 minutes, cool to 40°C, add the remaining raw materials, continue stirring at the same speed for 20 minutes, cool to room temperature, sterilize, and package to obtain the composition.

[0047] Example 3

[0048] The above-prepared composition was tested.

[0049] Log-phase B16-F10 melanoma cells (derived from C57BL / 6J mice) were cultured and cultured at a density of 1.5 × 10⁻⁶ cells / year. 6 Cells were seeded at 1 / mL in 6-well plates, and 2 mL of DMEM medium was added. The plates were incubated at 37°C with 5% CO2 for 24 hours. The culture medium was aspirated, and the cells were washed with PBS and discarded. Cells were then divided into groups (blank group, and combinations 1-5, 4 replicates per group). 2 mL of fresh DMEM medium was added to the blank group, and 2 mL of fresh DMEM medium (containing 1 mg / mL of the composition) was added to the corresponding composition groups. Cells were incubated at 37°C with 5% CO2 for 48 hours. The supernatant was discarded, and cells were collected. 1 mL of 10% DMSO solution (composition: 10% DMSO, 90% 1M sodium hydroxide solution, v / v) was added, and the cells were incubated at 85°C for 1 hour. After cooling to room temperature, the absorbance (OD) at 405 nm was measured. The melanin inhibition efficiency (ζ) of each group was calculated (ζ = (OD blank group - OD composition) / OD blank group × 100%). The Δζ value of each group compared to composition 5 was also calculated. Results are shown in Table 2 and [Table data would be inserted here]. Figure 3 .

[0050] Table 2: Whitening and Spot Removal Test Serial Number ζ △ζ Composition 1 32.83% -36.79% Composition 2 56.74% -12.88% Composition 3 58.57% -11.05% Composition 4 67.13% -2.49% Composition 5 69.62% /

[0051] As shown in Table 2 above, the composition prepared by this invention can effectively whiten and remove blemishes, giving cosmetics an excellent user experience. A comparison of compositions 1 and 4-5 shows that the use of licorice root extract and Saururus chinensis extract in the composition of this invention can effectively enhance the whitening and blemish-removing effects of the composition. Furthermore, a comparison of compositions 2-3 and 5 shows that the simultaneous use of licorice root extract and Saururus chinensis extract can synergistically enhance the whitening and blemish-removing effects, synergistically increasing the richness and variety of whitening active ingredients, thus giving the product excellent results.

[0052] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the present invention.

Claims

1. A skin whitening and freckle-removing skincare product composition, characterized in that, The raw material composition by weight is as follows: 0.05-0.5 parts of Bifida ferment lysate filtrate, 0.1-1.5 parts of Glycyrrhiza glabra extract, and 0.1-1.5 parts of Scutellaria baicalensis root extract.

2. The whitening and freckle-removing skincare composition according to claim 1, characterized in that, By weight, the raw materials also contain: 2-4 parts diglycerides, 8-15 parts glycerides, 2-6 parts polydimethylsiloxane, 0.1-1.5 parts sodium hyaluronate, 0.05-0.5 parts ascorbate glucoside, 0.02-0.3 parts ceramide NP and 60-90 parts water.

3. The whitening and freckle-removing skincare composition according to claim 1, characterized in that, By weight, the raw materials also contain: 0.1-1.5 parts of Saururus chinensis extract, 0.1-1.5 parts of Echinacea purpurea extract and 2-4.5 parts of emulsifier.

4. The whitening and freckle-removing skincare composition according to claim 3, characterized in that, The weight ratio of licorice root extract to Saururus chinensis extract is 1:0.6-1.

3.

5. The whitening and freckle-removing skincare composition according to claim 3, characterized in that, The emulsifier includes at least one of Tween-60, Tween-80, Tween-20, cetearyl alcohol polyether-20, and cocamidopropyl betaine.

6. The whitening and freckle-removing skincare composition according to claim 1, characterized in that, The preparation method of licorice root extract includes: crushing licorice root, mixing it with an ethanol solution of 20-35 wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract.

7. The whitening and freckle-removing skincare composition according to claim 1, characterized in that, The preparation method of the root extract of *Symplocos buergeriana* includes: crushing the root of *Symplocos buergeriana*, mixing it with an ethanol solution of 50-65 wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract.

8. The whitening and freckle-removing skincare composition according to claim 3, characterized in that, The preparation method of the extract of Saururus chinensis includes: taking Saururus chinensis, crushing it, then mixing it with an ethanol solution with a concentration of 20-35wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract; Alternatively, the preparation method of purple coneflower extract includes: taking purple coneflower, crushing it, then mixing it with an ethanol solution with a concentration of 50-65wt% in a certain proportion, heating and stirring to extract, and then filtering, concentrating and drying to obtain the extract.

9. A method for preparing a whitening and freckle-removing skincare composition according to any one of claims 1-8, characterized in that, The preparation method includes stirring and mixing the raw materials and sterilizing them.

10. The use of a whitening and freckle-removing skincare composition according to any one of claims 1-8 in the preparation of whitening or freckle-removing cosmetics.

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