Tetramethylpyrazine strain high-throughput breeding device

Abstract

The invention relates to the technical field of tetramethylpyrazine strain breeding, in particular to a tetramethylpyrazine strain high-throughput breeding device. Comprising a breeding box; a door plate is rotatably mounted on the breeding box, a handle is fixedly connected to the side wall of the door plate, a magnetic strip is embedded in the upper portion of the breeding box, a control panel is mounted on the side wall of the breeding box, ultraviolet lamps which are symmetrically distributed are mounted on the side wall in the breeding box, and a mounting frame is mounted at the bottom in the breeding box. And a mounting groove is formed in the mounting frame. The adding amount of nitrosoguanidine is accurately controlled through the quantitative assembly, the concentration and dosage of a mutagenic agent fed into each adding tank are consistent, the problem that the adding amount of nitrosoguanidine in traditional chemical mutagenesis is difficult to control in a standardized mode is solved, the difference of mutagenesis effects of strains in different batches is effectively avoided, the stability of the positive mutation rate of the strains is guaranteed, and the yield of the strains is increased. And the reliability of chemical mutagenesis is improved.

Description

Technical Field

[0001] This invention relates to the field of tetramethylpyrazine strain selection technology, and in particular to a high-throughput selection device for tetramethylpyrazine strains. Background Technology

[0002] Tetramethylpyrazine is a nitrogen-containing heterocyclic compound with important pharmacological activities and wide application value. It has good chemical stability and biological activity and is widely used in many fields such as cardiovascular and cerebrovascular drug intermediates, food flavorings, tobacco additives, feed additives and new materials.

[0003] Currently, high-yielding tetramethylpyrazine strains are mostly obtained through strain mutagenesis and selection. However, the existing methods for mutagenesis often involve placing the corresponding strain in a miniaturized culture plate and then using ultraviolet irradiation or the addition of chemical mutagens to induce the mutation of tetramethylpyrazine. In this process, it is difficult to standardize and control the amount of nitrosoguanidine added during chemical mutagenesis, which can easily lead to differences in the mutagenesis effect between different batches of strains, thus affecting the stability of the positive mutation rate. Furthermore, the miniaturized culture plate is prone to displacement when placed, resulting in uneven addition of chemical mutagens, which affects the mutagenesis effect and the accuracy of selection. Summary of the Invention

[0004] In order to overcome the shortcomings mentioned in the background art, the purpose of this invention is to provide a high-throughput breeding device for tetramethylpyrazine strains.

[0005] The technical solution is as follows: A high-throughput breeding device for tetramethylpyrazine strains includes a breeding box, a door panel rotatably mounted on the breeding box, a handle fixedly connected to the side wall of the door panel, a magnetic strip embedded in the upper part of the breeding box, a control panel mounted on the side wall of the breeding box, symmetrically distributed ultraviolet lamps mounted on the inner side wall of the breeding box, a mounting frame mounted at the bottom of the breeding box, a mounting groove formed on the mounting frame, a lead screw mounted in the mounting groove, a servo motor embedded in the mounting frame and connected to the lead screw, and a threaded connection to the lead screw. The nut is slidably connected to the mounting slot, and a mounting plate is fixedly attached to the bottom of the nut. A metering component for material addition is provided on the mounting plate. The inner wall of the selection box is provided with a trigger component that connects and cooperates with the metering component. The metering component includes adding tanks that are evenly distributed. A piston plate is slidably connected to the adding tank. A moving rod that penetrates the upper end of the adding tank is fixedly attached to the top of the piston plate. A pressure plate is fixedly attached between the upper ends of the evenly distributed moving rods. The ultraviolet lamp, the servo motor, and the metering component are all electrically connected to the control panel.

[0006] As an improvement to the above solution, the upper part of the side wall of the door panel near the magnetic strip is provided with a magnetic suction surface, which facilitates the use of the magnetic strip to seal the breeding box.

[0007] As an improvement to the above solution, the bottom of the addition tank is provided with a discharge port, and a membrane flap is provided inside the discharge port.

[0008] As an improvement to the above solution, the metering component further includes an electric telescopic rod installed at the bottom of the mounting plate. A fixed plate is installed at the telescopic end of the electric telescopic rod. Addition tanks distributed at equal intervals are embedded in the fixed plate. A symmetrically distributed spring telescopic rod is embedded in the fixed plate. The upper end of the spring telescopic rod is fixedly connected to the lower pressure plate. A storage tank is detachably connected to one side of the mounting plate. A pump body is detachably connected to the bottom of the storage tank. The inlet pipe of the pump body is detachably connected to the outlet of the storage tank. The outlet end of the pump body is connected to a conveying pipe. The conveying pipe is connected to a connecting pipe. The bottom of the connecting pipe is connected to equidistantly distributed conveying branch pipes. The conveying branch pipes are connected to adjacent addition tanks.

[0009] As an improvement to the above solution, the triggering component includes a fixed frame that is fixed to the rear wall of the breeding box and is equidistantly distributed. A transmission rack is installed on the side wall of the fixed plate. A sliding groove and a holding groove are sequentially opened on the fixed frame from top to bottom. A wedge plate is slidably connected in the sliding groove. A return spring is fixedly connected between the wedge plate and the breeding box. A rectangular groove that runs vertically through the wedge plate is provided inside the wedge plate. An oblique groove is opened on both sides of the rectangular groove. A sliding rod is slidably connected between the symmetrically distributed oblique grooves. A transmission component that is fixedly connected to the sliding rod is installed on the side wall of the holding groove.

[0010] As an improvement to the above solution, the transmission component includes a shaft rotatably connected to the inner side wall of the holding tank, a transmission gear that meshes with a transmission rack mounted on the shaft, a disc fixed to the end of the shaft, a pin fixed to an eccentric position on the side wall of the disc, a hollow frame sleeved on the outer side of the pin, a connecting rod fixed to the top of the hollow frame, the connecting rod being slidably connected to the fixing frame, and the upper end of the connecting rod being fixed to the sliding rod.

[0011] As an improvement to the above scheme, the bottom of the breeding box is provided with symmetrically distributed grooves. A swing plate is connected to the grooves through a rotating rod. A torsion spring is wound on the rotating rods on both sides of the swing plate. One end of the torsion spring is fixed to the swing plate, and the other end of the torsion spring is fixed to the groove. A locking groove is provided on the swing plate.

[0012] As an improvement to the above solution, a slot is provided at the bottom of the breeding box, and a support plate is placed in the slot. A sliding rod that penetrates the breeding box is fixed to the bottom of the support plate, and a connecting plate is fixed to the lower end of the sliding rod. A support spring is fixed between the connecting plate and the bottom of the breeding box. A straight rack is fixed to the connecting plate through a support rod. A spur gear that meshes with the spur gear is installed at the bottom of the breeding box through a bracket. An arc-shaped rack that meshes with the spur gear is fixed to the side wall of the door panel.

[0013] As an improvement to the above solution, the arc-shaped rack and the door panel are rotatably connected to the breeding box on the same axis.

[0014] As an improvement to the above solution, the arc-shaped rack and the straight rack are axially offset and both mesh with spur gears.

[0015] The present invention has the following advantages:

[0016] 1. This invention precisely controls the amount of nitrosoguanidine added through a quantitative component. The concentration of the mutagen fed into each addition tank is uniformly 20–100 μg / mL and the amount is consistent. This solves the problem of the difficulty in standardizing and controlling the amount of nitrosoguanidine added in traditional chemical mutagenesis, effectively avoids the difference in mutagenesis effect between different batches of strains, ensures the stability of the positive mutation rate of the strain, and improves the reliability of chemical mutagenesis.

[0017] 2. By using both ultraviolet physical mutagenesis and nitrosoguanidine chemical mutagenesis, the strain was first subjected to standardized ultraviolet irradiation for 10-60 seconds using an ultraviolet lamp, followed by precise addition of a chemical mutagen. The synergistic effect of the two mutagenesis methods significantly improved the positive mutation rate of the tetramethylpyrazine strain and increased the success rate of breeding high-yield strains.

[0018] 3. By setting up a swing plate with a locking slot inside the breeding box, the miniaturized culture plate can be automatically locked and fixed by its own weight pressing the swing plate during placement. This completely solves the problem of easy positional displacement of traditional culture plates, ensuring that the chemical mutagen is precisely aligned with the culture well when added, avoiding uneven addition of the mutagen, improving the mutagenesis effect and breeding accuracy. At the same time, in conjunction with the servo motor driving the precise lateral movement of the quantitative component, the precise alignment and addition of the mutagen is further realized.

[0019] 4. Through the arc-shaped rack and pinion, spur gear and spur rack transmission structure of the door panel linkage, the linkage control of the door panel opening and closing and the support plate lifting and lowering is realized. When the door panel is opened, the support plate rises automatically to facilitate the placement of the culture plate; when the door panel is closed, the support plate falls automatically to automatically lock the culture plate and the swing plate; after the selection is completed, the door panel is opened again and the support plate rises again to facilitate the removal of the culture plate. This design eliminates the step of manually adjusting the position of the culture plate and simplifies the operation process. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the present invention; Figure 2 This is a schematic diagram of the three-dimensional structure of the breeding box of the present invention; Figure 3 This is a cross-sectional three-dimensional structural diagram of the breeding box of the present invention; Figure 4 This is a three-dimensional structural diagram of the mounting bracket of the present invention; Figure 5 This is a three-dimensional structural diagram of the quantitative component of the present invention; Figure 6 This is a cross-sectional perspective view of the three-dimensional structure of the added tank of the present invention; Figure 7 for Figure 6 Enlarged 3D structural diagram at point A; Figure 8 This is a three-dimensional structural diagram of the fixing frame of the present invention; Figure 9 This is a cross-sectional perspective view of the fixing frame of the present invention. Figure 10 This is a schematic diagram of the three-dimensional structure of the magnetic strip of the present invention; Figure 11 This is a schematic diagram of the three-dimensional structure of the swing plate of the present invention; Figure 12 This is a schematic diagram of the three-dimensional structure of the door panel of the present invention.

[0021] Labels in the diagram: 1-Selection box, 2-Door panel, 3-Handle, 4-Magnetic strip, 5-Control panel, 6-UV lamp, 7-Mounting frame, 8-Mounting slot, 9-Lead screw, 10-Servo motor, 11-Nut, 12-Mounting plate, 13-Electric telescopic rod, 14-Fixing plate, 15-Adding tank, 151-Discharge port, 152-Diaphragm flap, 16-Piston plate, 17-Moving rod, 18-Lower pressure plate, 19-Spring telescopic rod, 20-Storage tank, 21-Pump body, 22-Conveying pipe, 23-Connecting pipe, 24- 25-Transmission rack, 26-Fixed frame, 27-Container trough, 28-Sliding groove, 29-Wedge plate, 30-Return spring, 31-Inclined groove, 32-Sliding rod, 33-Shaft, 34-Transmission gear, 35-Disc, 36-Pin, 37-Hollow frame, 38-Connecting rod, 39-Groove, 40-Swing plate, 41-Positioning groove, 42-Slotted, 43-Support plate, 44-Sliding rod, 45-Connecting plate, 46-Support spring, 47-Straight rack, 48-Arc rack, 49-Straight gear. Detailed Implementation

[0022] The technical solution will be further described below with reference to specific embodiments. It should be noted that the terms "up," "down," "left," and "right" used herein refer only to the position of the illustrated structure in the corresponding drawings. The component numbers used herein, such as "first," "second," etc., are merely for distinguishing the described objects and have no sequential or technical meaning. Furthermore, terms such as "connection" and "linkage," unless otherwise specified, include both direct and indirect connections.

[0023] Example 1

[0024] A high-throughput selection device for tetramethylpyrazine strains, such as Figures 1-9 As shown, the device includes a breeding box 1. A door panel 2 is rotatably mounted on the lower part of the front side of the breeding box 1. A handle 3 is bolted to the upper part of the front side wall of the door panel 2. A magnetic strip 4 is embedded in the upper part of the front side of the breeding box 1. The upper part of the rear side of the door panel 2 is set as a magnetic surface that magnetically engages with the magnetic strip 4. A control panel 5 is installed on the left side wall of the breeding box 1. Two ultraviolet lamps 6 are installed on the rear side wall inside the breeding box 1, arranged symmetrically. A mounting frame 7 is installed at the bottom inside the breeding box 1. The mounting frame 7 has a mounting groove 8. A lead screw 9 is installed in the mounting groove 8. A servo motor 10 connected to the lead screw 9 is embedded in the left part of the mounting frame 7. A nut 11 is threaded onto the lead screw 9 and slidably connected to the mounting groove 8. A mounting plate 12 is fixedly attached to the bottom of the selection box 1. A quantitative component for adding materials is provided on the mounting plate 12. A trigger component is provided on the inner wall of the selection box 1 to connect and cooperate with the quantitative component. The trigger component is used to control the delivery of quantitative materials in the quantitative component. The quantitative component includes adding tanks 15 that are evenly distributed. The bottom of the adding tank 15 has a discharge port 151. The discharge port 151 has four membrane flaps 152. A piston plate 16 is slidably connected inside the adding tank 15. A moving rod 17 that penetrates the upper end of the adding tank 15 is fixedly attached to the top of the piston plate 16. A lower pressure plate 18 is fixedly connected between the upper ends of the moving rods 17 that are evenly distributed. The ultraviolet lamp 6, the servo motor 10 and the quantitative component are all electrically connected to the control panel 5.

[0025] The metering component also includes an electric telescopic rod 13 installed at the bottom of the mounting plate 12. The telescopic end of the electric telescopic rod 13 is equipped with a fixed plate 14. The addition tanks 15, which are evenly distributed, are all embedded in the fixed plate 14. Two spring telescopic rods 19, which are symmetrically distributed front and back, are embedded in the fixed plate 14. The upper end of the spring telescopic rods 19 is fixedly connected to the lower pressure plate 18. The rear side of the mounting plate 12 is detachably connected to a storage tank 20. The bottom of the storage tank 20 is detachably connected to a pump body 21. The feed pipe of the pump body 21 is detachably connected to the outlet of the storage tank 20. The discharge end of the pump body 21 is connected to a conveying pipe 22. The conveying pipe 22 is connected to a connecting pipe 23. The bottom of the connecting pipe 23 is connected to an evenly distributed conveying branch pipe 24. The conveying branch pipe 24 is connected to the adjacent addition tank 15. When the pump body 21 is working, it can extract nitrosoguanidine from the storage tank 20 and send it into the addition tank 15 through the conveying pipe 22, the connecting pipe 23 and the conveying branch pipe 24.

[0026] The triggering component includes a fixed frame 26 fixed to the rear wall of the breeding box 1 and evenly distributed. A transmission rack 25 is installed on the rear side wall of the fixed plate 14. A sliding groove 28 and a holding groove 27 are sequentially opened from top to bottom on the fixed frame 26. A wedge plate 29 is slidably connected in the sliding groove 28. A return spring 30 is fixed between the rear side of the wedge plate 29 and the rear wall of the breeding box 1. A rectangular groove that runs vertically through the inside of the wedge plate 29 is provided. An inclined groove 31 is opened on the wedge plate 29 on both the left and right sides of the rectangular groove. A sliding rod 32 is slidably connected between the two inclined grooves 31. A transmission component fixed to the sliding rod 32 is installed on the right side wall of the holding groove 27.

[0027] The transmission component includes a shaft 33 rotatably connected to the right side wall of the holding tank 27. A transmission gear 34, which meshes with a transmission rack 25, is mounted on the shaft 33. A disc 35 is fixed to the left end of the shaft 33. A pin 36 is fixed to an eccentric position on the left side wall of the disc 35. A hollow frame 37 is sleeved on the outside of the pin 36. A connecting rod 38 is fixed to the top of the hollow frame 37. The connecting rod 38 is slidably connected to the fixed frame 26, and its upper end is fixed to the sliding rod 32. When the fixed plate 14 moves downward, it causes the transmission rack 25 to move downward. The downward movement of the transmission rack 25 will mesh with the transmission gear 34, causing the transmission gear 34 to rotate. The rotation of the transmission gear 34... The shaft 33 causes the disk 35 to rotate. The rotation of the disk 35 moves circumferentially through the pin 36 on it, pushing the hollow frame 37 downward. The downward movement of the hollow frame 37 causes the sliding rod 32 to move downward through the connecting rod 38, squeezing the inclined groove 31. This causes the wedge plate 29 to gradually move outward. The outward movement of the wedge plate 29 will squeeze the lower pressure plate 18 to move downward. The downward movement of the lower pressure plate 18 will push the piston plate 16 downward through the moving rod 17, increasing the pressure in the addition tank 15. This will cause the four membrane flaps 152 to open, discharging the nitrosoguanidine in the addition tank 15 through the discharge port 151 into the holes of the miniaturized culture plate. The nitrosoguanidine will then chemically mutate the bacterial strain placed in the miniaturized culture plate.

[0028] When performing high-throughput selection of tetramethylpyrazine strains, the user first selects appropriate amounts of Bacillus subtilis, Bacillus amyloliquefaciens, or Bacillus glutamate and places them into the wells of a miniaturized culture plate. Then, the user opens the selection box 1 by swinging the door panel 2 forward using handle 3. Next, the user places the miniaturized culture plate at the bottom of the selection box 1 and adjusts its position. After placement, the user resets the door panel 2 using handle 3. The magnetic attraction between the door panel 2 and the magnetic strip 4 seals the selection box 1. The user then activates the ultraviolet lamp 6 via control panel 5. The ultraviolet lamp 6 irradiates the strains placed in the miniaturized culture plate for 10-60 seconds, allowing the strains to undergo [the process / activation]. After physical mutagenesis and ultraviolet irradiation, the user operates the control panel 5 to turn off the ultraviolet lamp 6 and start the servo motor 10 and pump 21. The pump 21 extracts nitrosoguanidine from the storage tank 20 and delivers it to the addition tank 15 via the delivery pipe 22, connecting pipe 23, and delivery branch pipe 24 (the amount of nitrosoguanidine extracted can be controlled by the pump 21). The amount of nitrosoguanidine delivered to each addition tank 15 is the same (concentration 20–100 μg / mL). The servo motor 10 drives the lead screw 9 to rotate, causing the transmission nut 11 to move laterally (the servo motor starts intermittently, with each interval being 20 seconds, and each lateral movement is the same distance). The lateral movement of the nut 11 is controlled by the mounting plate 1. 2. The quantitative component moves. When the addition tank 15 on the quantitative component aligns with the arrangement holes of the miniaturized culture plate directly below, the servo motor 10 stops working, and the electric telescopic rod 13 works accordingly. The electric telescopic rod 13 drives the fixed plate 14 to reciprocate. The fixed plate 14 moves downward first, causing the addition tank 15 and the transmission rack 25 on it to move downward synchronously. During this process, the downward movement of the transmission rack 25 will mesh with the transmission gear 34, causing the transmission gear 34 to rotate. The rotation of the transmission gear 34 causes the disc 35 to rotate through the shaft 33. The rotation of the disc 35, through the circumferential movement of the pin 36 on it, can push the hollow frame 37 downward. The downward movement of the hollow frame 37, through the connecting rod 38, can... As the sliding rod 32 moves downward, it presses against the inclined groove 31, causing the wedge plate 29 to gradually move outward. The return spring 30 is then stretched. During the downward movement of the addition tank 15, as its lower end enters the holes of the miniaturized culture plate, the outward movement of the wedge plate 29 presses against the lower pressure plate 18, causing it to move downward. The spring in the spring extension rod 19 is compressed, and the downward movement of the lower pressure plate 18 pushes the piston plate 16 downward via the moving rod 17. This downward movement of the piston plate 16 increases the pressure inside the addition tank 15, causing the four membrane flaps 152 to open and discharge the nitrosoguanidine from the addition tank 15 through the outlet 151 into the holes of the miniaturized culture plate. The nitrosoguanidine then chemically induced mutagenesis in the bacterial strains placed in the miniaturized culture plate.As the fixed plate 14 moves upward, the addition tank 15 gradually moves away from the miniaturized culture plate. During this process, the downward pressure plate 18 moves upward, pushing the wedge plate 29 to gradually reset. The transmission rack 25 and transmission gear 34 cooperate to provide power for the hollow frame 37 to move and reset. At the same time, the spring force of the reset spring 30 facilitates the reset of the wedge plate 29. After the downward pressure plate 18 moves upward away from the wedge plate 29, the spring force on the spring extension rod 19 causes the downward pressure plate 18 to move upward and reset. For this purpose, the piston plate can be driven by the moving rod 17. After resetting, the electric telescopic rod 13 drives the fixed plate 14 to complete one reciprocating movement. At this time, the electric telescopic rod 13 closes while the servo motor 10 starts. This is to increase the positive mutation rate through the above two mutagenesis methods, making the mutagenized strain a tetramethylpyrazine strain. After all the strains in the miniaturized culture plate have completed mutagenesis, the user repeats the above operation to remove the miniaturized culture plate. Subsequently, the yield of the tetramethylpyrazine strain in the miniaturized culture plate is measured using an instrument. This method achieves high-throughput selection of tetramethylpyrazine strains.

[0029] Example 2

[0030] Based on Example 1, such as Figure 10 and Figure 11 As shown, two grooves 39 are symmetrically distributed at the bottom of the breeding box 1. A swing plate 40 is connected to the groove 39 by a rotating rod. Torsion springs are wound on the rotating rods on both sides of the swing plate 40. One end of the torsion spring is fixed to the swing plate 40, and the other end of the torsion spring is fixed to the groove 39. A locking groove 41 is provided on the swing plate 40.

[0031] When placing the miniaturized culture plate, it is placed between the inner sides of the two swing plates 40, and the miniaturized culture plate is pressed against the inner side plates of the two swing plates 40. Under the weight of the miniaturized culture plate itself, it squeezes the swing plates 40 to swing downward. The torsion spring on the swing plate 40 is tightened and stores force. By moving the miniaturized culture plate downward and cooperating with the locking groove 41 on the swing plate 40, the miniaturized culture plate can be fixed in place, avoiding the miniaturized culture plate from deviating and thus affecting the addition of nitrosoguanidine during chemical mutagenesis.

[0032] Example 3

[0033] Based on Example 2, such as Figure 12As shown, a slot 42 is provided at the bottom of the breeding box 1, and a support plate 43 is placed in the slot 42. A sliding rod 44 that passes through the breeding box 1 is fixed to the bottom of the support plate 43. A connecting plate 45 is fixed to the lower end of the sliding rod 44. A support spring 46 is fixed between the connecting plate 45 and the bottom of the breeding box 1. A rack 47 is fixed to the connecting plate 45 through a support rod. A spur gear 49 that meshes with the rack 47 is installed at the bottom of the breeding box 1 through a bracket. An arc-shaped rack 48 that meshes with the spur gear 49 is fixed to the side wall of the door panel 2. The arc-shaped rack 48 and the rack 47 are axially offset and both mesh with the spur gear 49. The arc-shaped rack 48 and the door panel 2 are rotatably connected to the breeding box 1 on the same axis.

[0034] During the downward swing of door panel 2 via handle 3, the swing of door panel 2 drives spur gear 49 to rotate via arc rack 48, which in turn drives spur rack 47 to move upward. The upward movement of spur rack 47 drives support plate 43 and slide rod 44 to move upward via support rod 45. The upward movement of slide rod 44 drives miniaturized culture plate to move upward via support plate 43, and the support spring 46 is compressed accordingly. The support plate 43 facilitates the placement of miniaturized culture plate. During the upward swing and reset process of door panel 2, arc rack 48 drives spur gear 49 to move spur rack 47 downward. The downward movement of spur rack 47 drives support plate 45, slide rod 44 and support plate 43 to move downward. This allows the miniaturized culture plate to move downward and automatically lock into place with swing plate 40. After the tetramethylpyrazine strain culture is completed, the downward swing of door panel 2 can automatically move the miniaturized culture plate upward, making it easy to pick up the miniaturized culture plate.

[0035] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A high-throughput selection device for tetramethylpyrazine strains, characterized in that, The system includes a selection box (1), on which a door panel (2) is rotatably mounted. A handle (3) is fixed to the side wall of the door panel (2). A magnetic strip (4) is embedded in the upper part of the selection box (1). A control panel (5) is installed on the side wall of the selection box (1). Ultraviolet lamps (6) are symmetrically distributed on the side wall inside the selection box (1). A mounting frame (7) is installed at the bottom inside the selection box (1). A mounting groove (8) is opened on the mounting frame (7). A lead screw (9) is installed in the mounting groove (8). A servo motor (10) connected to the lead screw (9) is embedded on the mounting frame (7). A threaded connection is made to the lead screw (9) that is slidably connected to the mounting groove (8). Nut (11), the bottom of the nut (11) is fixedly connected to the mounting plate (12), the mounting plate (12) is provided with a quantitative component for material addition, the inner wall of the selection box (1) is provided with a trigger component that is connected and cooperates with the quantitative component, the quantitative component includes an addition tank (15) distributed at equal intervals, a piston plate (16) is sealed and slidably connected inside the addition tank (15), a moving rod (17) that penetrates the upper end of the addition tank (15) is fixedly connected to the top of the piston plate (16), and a lower pressure plate (18) is fixedly connected between the upper ends of the moving rods (17) that are distributed at equal intervals, the ultraviolet lamp (6), the servo motor (10) and the quantitative component are all electrically connected to the control panel (5).

2. The high-throughput selection device for tetramethylpyrazine strains as described in claim 1, characterized in that, The upper part of the side wall of the door panel (2) near the magnetic strip (4) is provided with a magnetic suction surface, which facilitates the use of the magnetic strip (4) to close the breeding box (1).

3. The high-throughput selection device for tetramethylpyrazine strains as described in claim 2, characterized in that, The bottom of the addition tank (15) is provided with a discharge port (151), and a membrane flap (152) is provided inside the discharge port (151).

4. The high-throughput selection device for tetramethylpyrazine strains as described in claim 3, characterized in that, The quantitative assembly also includes an electric telescopic rod (13) installed at the bottom of the mounting plate (12). A fixed plate (14) is installed at the telescopic end of the electric telescopic rod (13). Addition tanks (15) distributed at equal intervals are embedded in the fixed plate (14). Spring telescopic rods (19) distributed symmetrically are embedded in the fixed plate (14). The upper end of the spring telescopic rod (19) is fixedly connected to the lower pressure plate (18). A storage tank is detachably connected to one side of the mounting plate (12). The storage tank (20) is detachably connected to a pump body (21) at its bottom. The feed pipe of the pump body (21) is detachably connected to the outlet of the storage tank (20). The discharge end of the pump body (21) is connected to a conveying pipe (22). The conveying pipe (22) is connected to a connecting pipe (23). The bottom of the connecting pipe (23) is connected to conveying branch pipes (24) that are evenly distributed. The conveying branch pipes (24) are connected to the adjacent addition tank (15).

5. The high-throughput selection device for tetramethylpyrazine strains as described in claim 4, characterized in that, The triggering component includes a fixed frame (26) fixed to the inner rear wall of the breeding box (1) and distributed at equal intervals. A transmission rack (25) is installed on the side wall of the fixed plate (14). A sliding groove (28) and a holding groove (27) are sequentially opened from top to bottom on the fixed frame (26). A wedge plate (29) is slidably connected in the sliding groove (28). A return spring (30) is fixed between the wedge plate (29) and the breeding box (1). A rectangular groove that runs vertically through the wedge plate (29) is provided inside the wedge plate (29). An inclined groove (31) is opened on both sides of the wedge plate (29). A sliding rod (32) is slidably connected between the symmetrically distributed inclined grooves (31). A transmission component fixed to the sliding rod (32) is installed on the side wall of the holding groove (27).

6. The high-throughput selection device for tetramethylpyrazine strains as described in claim 5, characterized in that, The transmission component includes a shaft (33) rotatably connected to the inner wall of the holding trough (27). A transmission gear (34) that meshes with a transmission rack (25) is mounted on the shaft (33). A disc (35) is fixedly connected to the end of the shaft (33). A pin (36) is fixedly connected to the eccentric position of the side wall of the disc (35). A hollow frame (37) is sleeved on the outside of the pin (36). A connecting rod (38) is fixedly connected to the top of the hollow frame (37). The connecting rod (38) is slidably connected to the fixed frame (26), and the upper end of the connecting rod (38) is fixedly connected to the sliding rod (32).

7. The high-throughput selection device for tetramethylpyrazine strains as described in claim 6, characterized in that, The bottom of the breeding box (1) is provided with symmetrically distributed grooves (39). A swing plate (40) is connected to the groove (39) by a rotating rod. A torsion spring is wound on the rotating rod on both sides of the swing plate (40). One end of the torsion spring is fixed to the swing plate (40), and the other end of the torsion spring is fixed to the groove (39). A locking groove (41) is provided on the swing plate (40).

8. The high-throughput selection device for tetramethylpyrazine strains as described in claim 7, characterized in that, The bottom of the breeding box (1) is provided with a slot (42), and a support plate (43) is placed in the slot (42). A sliding rod (44) that passes through the breeding box (1) is fixed to the bottom of the support plate (43). A connecting plate (45) is fixed to the lower end of the sliding rod (44). A support spring (46) is fixed between the connecting plate (45) and the bottom of the breeding box (1). A straight rack (47) is fixed to the connecting plate (45) through a support rod. A spur gear (49) that cooperates with the spur gear (47) is installed on the bottom of the breeding box (1) through a bracket. An arc-shaped rack (48) that cooperates with the spur gear (49) is fixed to the side wall of the door panel (2).

9. The high-throughput selection device for tetramethylpyrazine strains as described in claim 8, characterized in that, The arc-shaped rack (48) and the door panel (2) are rotatably connected to the breeding box (1) on the same axis.

10. The high-throughput selection device for tetramethylpyrazine strains as described in claim 9, characterized in that, The arc-shaped rack (48) and the straight rack (47) are axially offset and both mesh with the spur gear (49).

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