Biological membrane simulation device and method based on multiple flow velocity calibration and temperature control optimization
The biofilm simulation device, which utilizes multiple flow rate calibrations and temperature control optimizations, addresses the shortcomings of traditional biofilm reactors in temperature and flow rate control. It enables precise simulation of the pipeline environment and multi-material experiments, thereby improving the accuracy and completeness of experimental data.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUANGDONG RES INST OF WATER RESOURCES & HYDROPOWER
- Filing Date
- 2026-04-10
- Publication Date
- 2026-05-12
AI Technical Summary
Traditional biofilm reactors are not precise enough in temperature and flow rate control, making it difficult to simulate the environment in actual water pipelines, and also difficult to simulate multiple pipeline materials at the same time, affecting the accuracy and completeness of experimental data.
The biofilm simulation device employs multiple flow rate calibrations and temperature control optimizations, including a double-layered culture tank and an independent temperature control system. Water circulation is achieved through a peristaltic pump, and different pipe materials are simulated by a drive motor and a sample rack. The flow rate is monitored in real time using image processing algorithms, and biofilm characteristic data is analyzed through knowledge graphs.
It achieves precise control of pipe temperature and flow rate, can simulate various pipe materials, improves the accuracy and completeness of experimental data, and supports dynamic control and multi-group experimental verification.
Smart Images
Figure CN122012220A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of biofilm simulation devices, and more particularly to biofilm simulation devices and methods based on multiple flow rate calibrations and temperature control optimization. Background Technology
[0002] A biofilm reactor (BAR) is a laboratory device used to simulate the effects of various water quality parameters and hydraulic conditions on the biofilm on the pipe wall in a pipe network. Biofilm reactors can be used to conduct various studies on biofilm development characteristics and their impact on water quality. The reactor design varies depending on the experimental purpose. Typically, the reactor body consists of two nested acrylic cylinders. The inner cylinder holds the experimental water, with water inlets and outlets at the top and bottom. A rotor is installed inside the inner cylinder, and its speed can be adjusted by a frequency converter. The outer cylinder holds temperature-controlled water to regulate the temperature of the experimental water in the inner cylinder. Traditional biofilm reactor designs can meet most experimental needs, but they still have the following drawbacks and limitations: (1) Temperature has a significant impact on water quality in pipelines. Temperature affects the activity of microorganisms, thereby affecting their absorption and release of elements such as carbon, nitrogen, and phosphorus in the water. Therefore, precise temperature control is very important. Traditional designs use external sleeves for temperature control to regulate the temperature of experimental water in the internal sleeve. However, in actual deep-sea water pipeline projects, the pipe wall temperature and the water flow temperature differ significantly. To accurately simulate the process, it is necessary to improve the device to control the pipe wall temperature and the water flow temperature separately. (2) When the water flow velocity changes in the pipeline, the shear force on the wall changes, thereby affecting the structure of the biofilm and altering its mass transfer efficiency. Therefore, the reactor rotation speed control is crucial. Currently, frequency converters are generally used to adjust the rotation speed. Users need to perform unit conversion based on the actual water flow velocity in the pipeline (rotation speed unit is r / s, flow velocity unit is m / s). This method cannot be used to observe and judge in real time whether the rotation speed is accurately controlled. Therefore, it is necessary to invent an intuitive and controllable multi-velocity calibration device. (3) In the traditional design of biofilm reactors, a plate is usually inserted into the drum to simulate the pipe wall and then cultivate the biofilm. In this way, it is necessary to scrape and take samples for microbial detection. During operation, the biofilm structure and morphology will inevitably be damaged, making it difficult to observe the whole picture. Moreover, this method can only simulate one pipe material at a time and cannot obtain experimental data of multiple pipe wall materials at the same time. Summary of the Invention
[0003] This invention overcomes the shortcomings of the prior art and provides a biofilm simulation device and method based on multiple flow rate calibration and temperature control optimization.
[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows: The first aspect of this invention provides a biofilm simulation device based on multiple flow rate calibrations and temperature control optimization, comprising: The culture tank is divided into at least two layers. The first layer is connected to a temperature controller, which controls the temperature within the first layer. The second layer is connected to a peristaltic pump, which circulates the biofilm culture water. A drive motor is also installed on the top of the incubator. The output end of the drive motor is connected to the sample holder. Several sample pieces are installed on the sample holder. The surface of the sample pieces is coated to simulate the effect of different pipe materials on the experiment.
[0005] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the biofilm simulation device simulates the unidirectional flow rate of the pipeline during simulation. The stable flow rate environment makes the formed sheet-like biofilms similar to the pipeline-like biofilms.
[0006] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the surface of the biofilm simulation device is marked with scales, and the stable vortex formed on the water surface during operation is a cone.
[0007] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the current flow rate and rotation speed are calculated by observing the water surface changes of the biofilm simulation device in real time and measuring the height of the liquid surface variation.
[0008] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the biofilm simulation device is equipped with several control buttons.
[0009] A second aspect of this invention provides an analytical method for a biofilm simulation device based on multiple flow rate calibration and temperature control optimization, comprising the following steps: The living environment of a biofilm is constructed using a biofilm simulation device, real-time environmental data of the living environment of the biofilm is collected, and dynamic regulation is performed based on the real-time environmental data of the living environment of the biofilm. The absorption and release characteristics of the biofilm are also simulated. Through environmental simulation and verification, we obtain data on the absorption and release characteristics of biofilms under different organisms and different living environments, and construct a knowledge graph based on the data on the absorption and release characteristics of biofilms under different living environments. The living environment in the target area is obtained, and based on the living environment in the target area and the knowledge graph, the absorption and release characteristic data of biofilms of different biological types in the living environment of the target area are obtained. The preferred collection area for the target organism is generated based on the absorption and release characteristics of biofilms of different biological types in the target area's living environment.
[0010] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the living environment of the biofilm is constructed by the biofilm simulation device, real-time environmental data of the living environment of the biofilm is collected, and dynamic regulation is performed according to the real-time environmental data of the living environment of the biofilm. The absorption and release characteristics of the biofilm are simulated, specifically as follows: A biofilm living environment is constructed using a biofilm simulation device. Real-time environmental data of the biofilm living environment is collected. A threshold for environmental data change characteristics is set. Based on the real-time environmental data of the biofilm living environment, the real-time environmental data change characteristics within a preset time period are calculated. Determine whether the real-time environmental data change characteristics within the preset time period are greater than the environmental data change characteristic threshold; When the real-time environmental data change characteristics within the preset time period are greater than the environmental data change characteristic threshold, the real-time environmental data of the living environment of the biofilm are dynamically adjusted until they are no greater than the environmental data change characteristic threshold. When the real-time environmental data change characteristics within the preset time period are not greater than the environmental data change characteristic threshold, the real-time environmental data of the living environment of the biofilm are continuously monitored, and the absorption and release characteristic data of the biofilm are simulated.
[0011] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the absorption and release characteristic data of biofilms under different organisms and different living environments are obtained, and a knowledge graph is constructed based on the absorption and release characteristic data of biofilms under different living environments, specifically including: The absorption and release characteristics of biofilms under different organisms and different living environments are obtained, and the absorption and release characteristics of biofilms under different living environments are input into a graph neural network for processing. Using the living environment as the first graph node, the absorption and release characteristic data of biofilm as the second graph node, and the biological type as the third graph node, the first graph node, the second graph node, and the third graph node are connected to construct a topological structure graph and a knowledge graph. The topology graph is input into the knowledge graph for node representation.
[0012] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the living environment in the target area is obtained, and based on the living environment in the target area and the knowledge graph, the absorption and release characteristic data of biofilms of different biological types under the living environment in the target area are obtained, specifically including: The living environment in the target area is obtained, and the living environment in the target area is input into the knowledge graph for data matching. By matching data, the absorption and release characteristics of biofilms of different biological types in the target area are obtained, and the absorption and release characteristics of biofilms of different biological types in the target area are output.
[0013] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the preferred collection area for the target organism is generated according to the absorption and release characteristic data of biofilms of different biological types under the living environment in the target area, specifically including: Based on the absorption and release characteristic data of biofilms of different biological types in the target area under the living environment, the absorption and release characteristic data of biofilms of different biological types in each sub-region of the target area are obtained; Acquire the range information of absorption and release characteristic data of the target organism during its survival, and randomly select the preferred collection area of the target organism based on the absorption and release characteristic data of biofilms of different biological types in each sub-region of the target area; Acquire the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism, and determine whether the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are within the range of absorption and release characteristic data when the target organism is alive. When the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are within the range of absorption and release characteristic data when the target organism is alive, the preferred collection area of the target organism is output. When the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are not within the range of absorption and release characteristic data when the target organism is alive, the preferred collection area of the target organism is reset.
[0014] This invention addresses the shortcomings of the prior art and has the following beneficial effects: (1) This invention proposes a biofilm reactor for simulating water quality in water transmission pipelines. This device can simulate the effects of different temperatures, shear forces, pipe materials, etc., on water quality and biofilm growth in pipelines, and can be used to study the factors affecting water quality changes in water transmission pipelines; (2) Through multiple sets of experiments, this invention has achieved the effects of dynamic temperature control, precise speed adjustment, and direct detection under a microscope with multiple types of material hanging slides; (3) The feature of this invention is two independent temperature control systems, which can realize independent temperature control of the heat preservation liquid and the experimental water; the controller forms a stable vortex on the water surface into a cone, and the user can observe the changes in the water surface in real time, calculate the water flow rate according to the drop height of the liquid surface, and adjust the rotation speed or verify whether the rotation speed setting is accurate; the plate hanging device is a double-layer multi-directional rotating plate rack, which can load 8 plates of any substrate with a size of 60mm*30mm*2mm at one time, which is convenient for direct observation under a microscope without scraping the sample. Attached Figure Description
[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other embodiments can be obtained from these drawings without creative effort.
[0016] Figure 1 A schematic diagram of the control knob is shown; Figure 2 A schematic diagram of a membrane reactor is shown. Figure 3 A partial structural diagram of a biofilm reactor device is shown. Detailed Implementation
[0017] To better understand the above-mentioned objectives, features, and advantages of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner. Therefore, they only show the components related to the present invention. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other.
[0018] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as limiting the scope of protection of this application. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.
[0019] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art will understand the specific meaning of the above terms in this application based on the specific circumstances.
[0020] To facilitate understanding of the present invention, a more complete description will be given below with reference to the accompanying drawings. Preferred embodiments of the invention are shown in the drawings. However, the invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the invention.
[0021] like Figure 1 as well as Figure 3 As shown, the first aspect of the present invention provides a biofilm simulation device based on multiple flow rate calibration and temperature control optimization, comprising: The culture tank 1 is divided into at least two layers. The first layer is connected to the temperature controller 2 (forming a circulation system through connecting pipes) and the temperature value in the first layer is controlled by the temperature controller 2. The second layer is connected to the peristaltic pump 4 (forming a circulation system through connecting pipes) and the peristaltic pump 4 realizes the circulation of biofilm culture water. It should be noted that this device is designed as a dual-system, meaning that both the inner and outer sleeves (the first and second layers) can be heated and insulated. Through two independent temperature control systems, the external insulation solution and the internal experimental water can be controlled independently, enabling both static and dynamic culture modes. This effectively ensures stable ambient temperature and achieves rapid temperature control. Using a semiconductor temperature sensor, a controllable temperature range of -60℃ to 120℃ is achieved, with an error of only 0.3 degrees Celsius.
[0022] It should be noted that this device alters the internal culture environment to obtain data on the absorption and release characteristics of different biofilm types under different environments (such as data on the absorption and release of elements such as carbon, nitrogen, and phosphorus in water by microorganisms). A drive motor 3 is also installed on the top of the culture tank 1. The output end of the drive motor 3 is connected to the test piece rack 5. Several test pieces are installed on the test piece rack 5. A coating is added to the surface of the test pieces to simulate the influence of different pipe materials on the experiment.
[0023] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the biofilm simulation device simulates the unidirectional flow rate of the pipeline during simulation. The stable flow rate environment makes the formed sheet-like biofilms similar to the pipeline-like biofilms.
[0024] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the surface of the biofilm simulation device is marked with scales, and the stable vortex formed on the water surface during operation is a cone.
[0025] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the current flow rate and rotation speed are calculated by observing the water surface changes of the biofilm simulation device in real time and measuring the height of the liquid surface variation.
[0026] It should be noted that this device has an independent, high-precision, continuously variable speed control power system. The stable vortex generated during its operation can completely simulate the unidirectional flow velocity of a pipe. This stable flow environment makes the resulting membrane organisms highly similar to those in the pipe. The device is designed with graduated markings on its surface. During operation, the stable vortex formed on the water surface is conical, allowing users to observe water level changes in real time. By observing the height of the liquid level fluctuations, the current flow rate and rotation speed can be directly calculated, further verifying the accuracy of the rotation speed setting. Alternatively, the knob can be adjusted according to water level changes until the ideal rotation speed is achieved.
[0027] By setting multiple sets of parameters, the forward or reverse rotation of water flow, time control, signal control, etc., can be realized, achieving stepless speed regulation within the 0-N range, and further realizing dynamic simulation of pipeline water flow and even a wider range.
[0028] Simultaneously, a high-speed camera above the instrument captures natural textures on the water surface (such as ripples, bubbles, or floating objects). Image processing algorithms analyze the displacement of feature points in consecutive frames to calculate the flow velocity. The accuracy of the results is further verified by comparing non-contact image flow measurement with this method.
[0029] The device innovatively features a double-layer, multi-directional rotating sample holder in its internal sleeve, which can hold up to eight sample pieces of any substrate with a size of 60mm*30mm*2mm at a time. This size allows for direct observation under a microscope without the need to scrape the sample, thus avoiding damage to biofilms and other tissues.
[0030] The sample holder makes full use of the experimental space, and can obtain 8 sets of samples in one experiment, acquiring sample data from all aspects inside the simulated pipeline; and the surface of the sample can be coated as needed, so that the influence of different pipeline materials can be simulated in one experiment.
[0031] Furthermore, in the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the biofilm simulation device is equipped with several control buttons.
[0032] In summary, this invention proposes a biofilm reactor for simulating water quality in water pipelines. This device can simulate the effects of different temperatures, shear forces (adjusting the operating parameters of the drive motor, such as power and speed), and pipe materials on water quality and biofilm growth, enabling research on factors influencing water quality changes in water pipelines. Through multiple experimental verifications, this invention achieves dynamic temperature control, precise speed adjustment, and direct microscopic detection of various material samples. The invention is characterized by two independent temperature control systems, enabling independent temperature control of the insulation liquid and experimental water. The controller forms a stable vortex cone on the water surface, allowing users to observe water level changes in real time and calculate the water flow velocity based on the liquid level drop height to adjust the speed or verify the accuracy of the speed setting. The sample holder is a double-layered, multi-directional rotating sample rack that can hold eight sample pieces of any substrate with a size of 60mm*30mm*2mm at a time, facilitating direct microscopic observation without the need for sample scraping.
[0033] like Figure 1 As shown, it should be noted that the device has four knobs: F1, F2, F3, and F4. F1 represents "stop temperature," where the internal program automatically determines whether to heat or cool. F2 represents "hysteresis value," where the heating and cooling relays will not conduct within the range of the stop temperature ± temperature difference. F3 represents cooling delay, and the Cool indicator light will flash when the delay is activated. F4 represents "temperature calibration," where the displayed temperature equals the temperature before calibration plus the calibration temperature.
[0034] A second aspect of this invention provides an analytical method for a biofilm simulation device based on multiple flow rate calibration and temperature control optimization, comprising the following steps: The living environment of a biofilm is constructed using a biofilm simulation device, real-time environmental data of the living environment of the biofilm is collected, and dynamic regulation is performed based on the real-time environmental data of the living environment of the biofilm. The absorption and release characteristics of the biofilm are also simulated. Through environmental simulation and verification, we obtain data on the absorption and release characteristics of biofilms under different organisms and different living environments, and construct a knowledge graph based on the data on the absorption and release characteristics of biofilms under different living environments. The living environment in the target area is obtained, and based on the living environment in the target area and the knowledge graph, the absorption and release characteristic data of biofilms of different biological types in the living environment of the target area are obtained. The preferred collection area for the target organism is generated based on the absorption and release characteristics of biofilms of different biological types in the target area's living environment.
[0035] It should be noted that this method can generate the optimal collection area for target organisms based on the absorption and release characteristics of biofilms of different biological types in the target area's living environment, thereby optimizing the collection area for target microorganisms and improving the collection efficiency of target microorganisms.
[0036] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the living environment of the biofilm is constructed by the biofilm simulation device, real-time environmental data of the living environment of the biofilm is collected, and dynamic regulation is performed according to the real-time environmental data of the living environment of the biofilm. The absorption and release characteristics of the biofilm are simulated, specifically as follows: A biofilm living environment is constructed using a biofilm simulation device. Real-time environmental data of the biofilm living environment is collected. A threshold for environmental data change characteristics is set. Based on the real-time environmental data of the biofilm living environment, the real-time environmental data change characteristics within a preset time period are calculated. Determine whether the real-time environmental data change characteristics within the preset time period are greater than the environmental data change characteristic threshold; When the real-time environmental data change characteristics within the preset time period are greater than the environmental data change characteristic threshold, the real-time environmental data of the living environment of the biofilm are dynamically adjusted until they are no greater than the environmental data change characteristic threshold (e.g., if the temperature change is too large, the threshold is set to exceed 3°C). When the real-time environmental data change characteristics within the preset time period are not greater than the environmental data change characteristic threshold, the real-time environmental data of the living environment of the biofilm are continuously monitored, and the absorption and release characteristic data of the biofilm are simulated.
[0037] It should be noted that this method can generate a preferred collection area for the target organism based on the absorption and release characteristics of biofilms of different biological types in the target area's living environment.
[0038] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the absorption and release characteristic data of biofilms under different organisms and different living environments are obtained, and a knowledge graph is constructed based on the absorption and release characteristic data of biofilms under different living environments, specifically including: The absorption and release characteristic data of biofilms under different organisms and different living environments (descriptions such as temperature, humidity, salinity, etc.) are obtained, and the absorption and release characteristic data of biofilms under different living environments are input into a graph neural network for processing. Using the living environment as the first graph node, the absorption and release characteristic data of biofilm as the second graph node, and the biological type as the third graph node, the first graph node, the second graph node, and the third graph node are connected to construct a topological structure graph and a knowledge graph. The topology graph is input into the knowledge graph for node representation.
[0039] It should be noted that this method can generate living environment data, biofilm absorption and release characteristic data, and topological structure diagrams of biological types, thereby forming a knowledge graph to represent the relationships between living environment data, biofilm absorption and release characteristic data, and topological structure diagrams of biological types.
[0040] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the living environment in the target area is obtained, and based on the living environment in the target area and the knowledge graph, the absorption and release characteristic data of biofilms of different biological types under the living environment in the target area are obtained, specifically including: The living environment in the target area is obtained, and the living environment in the target area is input into the knowledge graph for data matching. By matching data, the absorption and release characteristics of biofilms of different biological types in the target area are obtained, and the absorption and release characteristics of biofilms of different biological types in the target area are output.
[0041] Furthermore, in the analysis method of the biofilm simulation device based on multiple flow rate calibration and temperature control optimization, the preferred collection area for the target organism is generated according to the absorption and release characteristic data of biofilms of different biological types under the living environment in the target area, specifically including: Based on the absorption and release characteristic data of biofilms of different biological types in the target area under the living environment, the absorption and release characteristic data of biofilms of different biological types in each sub-region of the target area are obtained; Acquire the range information of absorption and release characteristic data of the target organism during its survival, and randomly select the preferred collection area of the target organism based on the absorption and release characteristic data of biofilms of different biological types in each sub-region of the target area; Acquire the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism, and determine whether the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are within the range of absorption and release characteristic data when the target organism is alive. When the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are within the range of absorption and release characteristic data when the target organism is alive, the preferred collection area of the target organism is output. When the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are not within the range of absorption and release characteristic data when the target organism is alive, the preferred collection area of the target organism is reset.
[0042] It should be noted that this method can generate the optimal collection area for target organisms based on the absorption and release characteristics of biofilms of different biological types in the target area's living environment, thereby optimizing the collection area for target microorganisms and improving the collection efficiency of target microorganisms.
[0043] In addition, this method also includes: A meteorological feature prediction model is constructed based on a deep neural network. Historical meteorological feature data information of the target area is obtained, and the historical meteorological feature data information of the target area is input into the meteorological feature prediction model for training. Through training, a trained meteorological feature prediction model is obtained, meteorological feature data information of the target area within a preset time is obtained, and the meteorological feature data information of the target area within the preset time is input into the trained meteorological feature prediction model for prediction. By prediction, meteorological characteristic data information of the target area within the next preset time is obtained, and the meteorological characteristic data information of the target area within the next preset time is input into the knowledge graph for data matching; By matching data, the absorption and release characteristics of biofilms in the target area within the next preset time period are obtained. The time node in which the biofilm absorption and release characteristic data is located within the range of absorption and release characteristic data during the survival of the target organism is obtained from the biofilm absorption and release characteristic data corresponding to the meteorological characteristic data information in the target area within the next preset time, and the time node is used as the collection node of the target organism.
[0044] It should be noted that this method fully considers environmental changes to obtain the time point where the biofilm absorption and release characteristic data information falls within the range of absorption and release characteristic data information during the survival of the target organism. The time point is used as the collection point of the target organism, thereby improving the rationality of the collection of target microorganisms.
[0045] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.
[0046] The embodiments described above are merely specific implementations of this application, used to illustrate the technical solutions of this application, and not to limit it. The protection scope of this application is not limited thereto. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments, or make equivalent substitutions for some of the technical features, within the scope of the technology disclosed in this application; and these modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application. All should be covered within the protection scope of this application. Therefore, the protection scope of this application should be determined by the protection scope of the claims.
Claims
1. A biofilm simulation device based on multiple flow rate calibrations and temperature control optimization, characterized in that, include: The culture tank is divided into at least two layers. The first layer is connected to a temperature controller, which controls the temperature within the first layer. The second layer is connected to a peristaltic pump, which circulates the biofilm culture water. A drive motor is also installed on the top of the incubator. The output end of the drive motor is connected to the sample holder. Several sample pieces are installed on the sample holder. The surface of the sample pieces is coated to simulate the effect of different pipe materials on the experiment.
2. The biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 1, characterized in that, During simulation, the biofilm simulation device simulates the unidirectional flow velocity of the pipe, and the stable flow velocity environment ensures that the formed sheet-like organisms are similar to the pipe-like organisms.
3. The biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 1, characterized in that, The surface of the biofilm simulation device is marked with graduations, and the stable vortex formed on the water surface during operation is in the shape of a cone.
4. The biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 1, characterized in that, By observing the water surface changes in the biofilm simulation device in real time, the current flow rate and rotation speed can be calculated based on the height of the liquid surface fluctuation.
5. The biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 1, characterized in that, The biofilm simulation device is equipped with several control buttons in Shanghai.
6. An analytical method for a biofilm simulation device based on multiple flow rate calibration and temperature control optimization, characterized in that, The biofilm simulation device based on multiple flow rate calibration and temperature control optimization as described in any one of claims 1-5 includes the following steps: The living environment of a biofilm is constructed using a biofilm simulation device, real-time environmental data of the living environment of the biofilm is collected, and dynamic regulation is performed based on the real-time environmental data of the living environment of the biofilm. The absorption and release characteristics of the biofilm are also simulated. Through environmental simulation and verification, we obtain data on the absorption and release characteristics of biofilms under different organisms and different living environments, and construct a knowledge graph based on the data on the absorption and release characteristics of biofilms under different living environments. The living environment in the target area is obtained, and based on the living environment in the target area and the knowledge graph, the absorption and release characteristic data of biofilms of different biological types in the living environment of the target area are obtained. The preferred collection area for the target organism is generated based on the absorption and release characteristics of biofilms of different biological types in the target area's living environment.
7. The analytical method for the biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 6, characterized in that, A biofilm simulation device is used to construct the living environment of a biofilm, collect real-time environmental data of the biofilm's living environment, and dynamically adjust the environment based on this data. The absorption and release characteristics of the biofilm are also simulated. Specifically: A biofilm living environment is constructed using a biofilm simulation device. Real-time environmental data of the biofilm living environment is collected. A threshold for environmental data change characteristics is set. Based on the real-time environmental data of the biofilm living environment, the real-time environmental data change characteristics within a preset time period are calculated. Determine whether the real-time environmental data change characteristics within the preset time period are greater than the environmental data change characteristic threshold; When the real-time environmental data change characteristics within the preset time period are greater than the environmental data change characteristic threshold, the real-time environmental data of the living environment of the biofilm are dynamically adjusted until they are no greater than the environmental data change characteristic threshold. When the real-time environmental data change characteristics within the preset time period are not greater than the environmental data change characteristic threshold, the real-time environmental data of the living environment of the biofilm are continuously monitored, and the absorption and release characteristic data of the biofilm are simulated.
8. The analytical method for the biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 6, characterized in that, Acquire data on the absorption and release characteristics of biofilms under different organisms and different living environments, and construct a knowledge graph based on the data on the absorption and release characteristics of biofilms under different living environments, specifically including: The absorption and release characteristics of biofilms under different organisms and different living environments are obtained, and the absorption and release characteristics of biofilms under different living environments are input into a graph neural network for processing. Using the living environment as the first graph node, the absorption and release characteristic data of biofilm as the second graph node, and the biological type as the third graph node, the first graph node, the second graph node, and the third graph node are connected to construct a topological structure graph and a knowledge graph. The topology graph is input into the knowledge graph for node representation.
9. The analytical method for the biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 6, characterized in that, The system acquires the living environment of the target area and, based on the living environment and knowledge graph, obtains data on the absorption and release characteristics of biofilms of different biological types within the living environment of the target area. Specifically, this includes: The living environment in the target area is obtained, and the living environment in the target area is input into the knowledge graph for data matching. By matching data, the absorption and release characteristics of biofilms of different biological types in the target area are obtained, and the absorption and release characteristics of biofilms of different biological types in the target area are output.
10. The analytical method for the biofilm simulation device based on multiple flow rate calibration and temperature control optimization according to claim 6, characterized in that, Based on the absorption and release characteristics of biofilms of different biological types in the target area, a preferred collection area for the target organism is generated, specifically including: Based on the absorption and release characteristic data of biofilms of different biological types in the target area under the living environment, the absorption and release characteristic data of biofilms of different biological types in each sub-region of the target area are obtained; Acquire the range information of absorption and release characteristic data of the target organism during its survival, and randomly select the preferred collection area of the target organism based on the absorption and release characteristic data of biofilms of different biological types in each sub-region of the target area; Acquire the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism, and determine whether the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are within the range of absorption and release characteristic data when the target organism is alive. When the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are within the range of absorption and release characteristic data when the target organism is alive, the preferred collection area of the target organism is output. When the absorption and release characteristic data of biofilms of different biological types in the preferred collection area of each target organism are not within the range of absorption and release characteristic data when the target organism is alive, the preferred collection area of the target organism is reset.