Organ-like chip platform for simulating multi-organ interaction

Abstract

The organ-like chip platform for simulating multi-organ interaction comprises a culture dish, the outer side of the culture dish is covered with a supporting frame, the upper end of the supporting frame is fixedly connected with a guide frame, a first movable guide strip and a second movable guide strip are installed on the guide frame in a sliding mode, and the first movable guide strip and the second movable guide strip are in movable contact with each other. A movable supporting seat is movably arranged at the intersection of the first movable guide strip and the second movable guide strip in a sleeved mode, and an ear washing bulb is inserted into the movable supporting seat. The moving position of the movable supporting seat is manually operated, and the dripping position of the ear washing bulb is adjusted; after the ear washing bulb is taken down to suck a target culture solution, the ear washing bulb is inserted back into the movable supporting seat, a palm is placed on the movable supporting seat, the ear washing bulb is pressed by fingers, so that the ear washing bulb drips the target culture solution into a target position of a culture dish, and the ear washing bulb is positioned by the movable supporting seat, so that hand shaking and inclined dripping are avoided, the operation of a green hand is facilitated, and the accuracy is improved.

Description

Technical Field

[0001] This invention belongs to the field of medical device technology, and in particular relates to an organ-on-a-chip platform that simulates multi-organ interactions. Background Technology

[0002] Organ-on-a-chip (also known as "human chip" or "multi-organ chip") that simulates multi-organ interactions is an integrated microfluidic biomimetic system developed on the basis of single-organ organ-on-a-chip. Its core is to precisely connect two or more functional organ-on-a-chip units (such as liver, kidney, heart, intestine, brain, etc.) through a microchannel network, simulating the material exchange process of blood / body fluid circulation in the human body. This allows different organ units to achieve cross-organ "crosstalk" by transmitting signals such as metabolic products, cytokines, and drug molecules, thereby recreating the physiological scenario of multi-organ collaborative work in the human body.

[0003] When adding culture medium to organoid microarrays, skilled senior physicians are needed to ensure their hands do not tremble. Novices who only have theoretical knowledge are prone to misplacing the culture medium, resulting in low accuracy when adding culture medium to organoid microarrays.

[0004] To address these issues, we propose an organ-on-a-chip platform that simulates multi-organ interactions. Summary of the Invention

[0005] The purpose of this invention is to solve the problem of low accuracy in dropping culture medium into organoid microarrays by beginners, and to propose an organoid microarray platform that simulates multi-organ interactions.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: An organoid chip platform simulating multi-organ interactions includes a culture dish. A support frame covers the outside of the culture dish, and a guide frame is fixedly connected to the upper end of the support frame. A first movable guide strip and a second movable guide strip are slidably mounted on the guide frame, and the first and second movable guide strips are in contact with each other. A movable support seat is movably fitted at the intersection of the first and second movable guide strips, and a syringe bulb is inserted into the movable support seat. The support frame is placed over the culture dish, and the movable support seat is manually moved to adjust the position of the syringe bulb. After removing the syringe bulb and drawing the target culture medium, the syringe bulb is inserted back into the movable support seat. The palm is placed on the movable support seat, and the syringe bulb is pressed with the fingers to drip the target culture medium into the target position in the culture dish. The movable support seat positions the syringe bulb, preventing shaky drips and improving accuracy for novice operators.

[0007] Preferably, a suction cup is fixedly connected to the lower end of the support frame. The suction cup, in conjunction with the smooth surface of the target table, increases the stability of the support frame when placed on the smooth surface.

[0008] Preferably, the support frame includes a frame body, with connecting posts fixedly connected to the corners of the frame body. A fixing hole is provided at the lower end of each connecting post, and the upper end of the suction cup is fixedly installed within the fixing hole. The connecting posts increase the stability of the frame body's corners.

[0009] Preferably, the guide frame includes a first guide rod and a second guide rod, each in pair. The side ends of the first and second guide rods are fixedly connected, and the lower ends of the first and second guide rods are fixedly connected to the upper ends of the frame and the connecting column. The first guide rod guides the first movable guide bar to slide, and the second guide rod guides the second movable guide bar to slide.

[0010] Preferably, the first movable guide strip includes a first strip body, and a first sliding sleeve is fixedly connected to the side end of the first strip body. The first sliding sleeve is slidably fitted onto the first guide rod. The first guide rod guides the sliding sleeve to slide.

[0011] Preferably, the second movable guide strip includes a second strip body, and a second sliding sleeve is fixedly connected to the side end of the second strip body. The second sliding sleeve is slidably mounted on the second guide rod. The second guide rod guides the second sliding sleeve to slide.

[0012] Preferably, the movable support includes a base body, which is slidably mounted on the first and second strips. A collar is fixedly connected to the side end of the base body, and a connecting piece is fixedly connected to the side end of the base body. A pad is fixedly connected to the connecting piece. The collar restrains the syringe bulb, and the hand is placed on the pad to prevent hand tremors and improve the stability of operating the syringe bulb.

[0013] Preferably, the bulb syringe includes a glass tube inserted into a collar, a retaining ring fixedly connected to the middle of the glass tube, the retaining ring blocking the collar, and an elastic airbag fixedly connected to the upper end of the glass tube. The glass tube is inserted into the collar, and the retaining ring maintains the height of the bulb syringe.

[0014] In summary, the technical effects and advantages of this invention are as follows: 1. Place the support frame over the culture dish, manually move the movable support base to adjust the position of the syringe bulb; remove the syringe bulb to absorb the target culture medium, then insert the syringe bulb back into the movable support base. Place your palm on the movable support base and press the syringe bulb with your fingers to allow the syringe bulb to drip the target culture medium into the target position in the culture dish. Positioning the syringe bulb using the movable support base avoids hand tremors and ensures accuracy for beginners.

[0015] 2. Use the collar to restrain the syringe bulb, and place your hand on the pad block to prevent hand tremors and improve the stability of operating the syringe bulb. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the support frame structure of the present invention; Figure 3 This is a schematic diagram of the guide frame structure of the present invention; Figure 4 This is a schematic diagram of the first movable guide strip structure of the present invention; Figure 5 This is a schematic diagram of the second active guide strip structure of the present invention; Figure 6 This is a schematic diagram of the movable support structure of the present invention; Figure 7 This is a schematic diagram of the ear syringe structure of the present invention.

[0017] In the diagram: 1. Petri dish; 2. Support frame; 3. Guide frame; 4. First movable guide bar; 5. Second movable guide bar; 6. Movable support base; 7. Bulb; 8. Suction cup; 21. Frame; 22. Connecting column; 23. Fixing hole; 31. First guide rod; 32. Second guide rod; 41. First strip; 42. First sliding sleeve; 51. Second strip; 52. Second sliding sleeve; 61. Seat; 62. Connecting piece; 63. Pad block; 64. Collar; 71. Glass tube; 72. Retaining ring; 73. Elastic airbag. Detailed Implementation

[0018] The technical solutions in the embodiments of the invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the invention, and not all embodiments.

[0019] like Figure 1 As shown, an organoid chip platform simulating multi-organ interactions includes a culture dish 1. A support frame 2 covers the outside of the culture dish 1. A guide frame 3 is fixedly connected to the upper end of the support frame 2. A first movable guide strip 4 and a second movable guide strip 5 are slidably mounted on the guide frame 3. The first and second movable guide strips 4 and 5 are in contact with each other. A movable support seat 6 is movably fitted at the intersection of the first and second movable guide strips 4 and 5. A syringe bulb 7 is inserted into the movable support seat 6. The support frame 2 is placed over the culture dish 1, and the movable support seat 6 is manually moved to adjust the position of the syringe bulb 7. After removing the syringe bulb 7 to absorb the target culture medium, the syringe bulb 7 is inserted back into the movable support seat 6. The palm is placed on the movable support seat 6, and the syringe bulb 7 is pressed with the fingers to drip the target culture medium into the target position of the culture dish 1.

[0020] like Figure 1As shown, a suction cup 8 is fixedly connected to the lower end of the support frame 2. The suction cup 8 is used to increase the stability of the support frame 2 on the smooth table surface.

[0021] like Figure 1 and 2 As shown, the support frame 2 includes a frame body 21, with connecting posts 22 fixedly connected to the corners of the frame body 21. A fixing hole 23 is provided at the lower end of the connecting post 22, and the upper end of the suction cup 8 is fixedly installed in the fixing hole 23. The connecting posts 22 are used to increase the stability of the corners of the frame body 21.

[0022] like Figure 1 , 2 As shown in Figure 3, the guide frame 3 includes a first guide rod 31 and a second guide rod 32. Each guide rod 31 and the second guide rod 32 is provided in pairs. The side ends of the first guide rod 31 and the second guide rod 32 are fixedly connected. The lower ends of the first guide rod 31 and the second guide rod 32 are fixedly connected to the upper ends of the frame 21 and the connecting column 22. The first guide rod 31 guides the first movable guide bar 4 to slide, and the second guide rod 32 guides the second movable guide bar 5 to slide.

[0023] like Figure 1 , 3 As shown in Figure 4, the first movable guide bar 4 includes a first bar body 41, and a first sliding sleeve 42 is fixedly connected to the side end of the first bar body 41. The first sliding sleeve 42 is slidably sleeved on the first guide rod 31. The first guide rod 31 guides the first sliding sleeve 42 to slide.

[0024] like Figure 1 , 3 As shown in Figure 5, the second movable guide bar 5 includes a second bar body 51, and a second sliding sleeve 52 is fixedly connected to the side end of the second bar body 51. The second sliding sleeve 52 is slidably mounted on the second guide rod 32. The second sliding sleeve 52 is guided to slide by the second guide rod 32.

[0025] like Figure 1 , 4 As shown in Figures 5 and 6, the movable support 6 includes a seat body 61, which is slidably mounted outside the first body 41 and the second body 51. A collar 64 is fixedly connected to the side end of the seat body 61, and a connecting piece 62 is fixedly connected to the side end of the seat body 61. A pad block 63 is fixedly connected to the connecting piece 62. The collar 64 is used to restrain the ear syringe bulb 7, and the hand is placed on the pad block 63 to prevent hand tremors.

[0026] like Figure 1 , 6As shown in Figure 7, the ear syringe 7 includes a glass tube 71, which is inserted into a collar 64. A retaining ring 72 is fixedly connected to the middle of the glass tube 71, and the retaining ring 72 is blocked on the collar 64. An elastic airbag 73 is fixedly connected to the upper end of the glass tube 71. The glass tube 71 is inserted into the collar 64, and the retaining ring 72 is used to maintain the height of the ear syringe 7.

[0027] Working principle: Cover the culture dish 1 with the support frame 2, manually move the movable support 6 to adjust the position of the syringe bulb 7; remove the syringe bulb 7 to absorb the target culture medium, insert the syringe bulb 7 back into the movable support 6, place your palm on the movable support 6, and press the syringe bulb 7 with your fingers to make the syringe bulb 7 drip the target culture medium into the target position of the culture dish 1.

[0028] The above description is merely a preferred embodiment of the invention, but the scope of protection of the invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the invention, based on the technical solution and inventive concept of the invention, should be included within the scope of protection of the invention.

[0029] The description briefly mentions the application directions of the invention to existing technologies known to those skilled in the art without modification, which are combined with the invention to form a complete technology; it avoids excessive popularization of technologies familiar to those skilled in the art, in order to help those skilled in the art quickly understand the main content of the invention.

Claims

1. An organ-on-a-chip platform for simulating multi-organ interactions, comprising a culture dish (1), characterized in that: The outer side of the petri dish (1) is covered by a support frame (2), and the upper end of the support frame (2) is fixedly connected to a guide frame (3). A first movable guide strip (4) and a second movable guide strip (5) are slidably installed on the guide frame (3). The first movable guide strip (4) and the second movable guide strip (5) are in contact with each other. A movable support seat (6) is movably fitted at the intersection of the first movable guide strip (4) and the second movable guide strip (5). A bulb syringe (7) is inserted into the movable support seat (6).

2. The organ-on-a-chip platform for simulating multi-organ interactions according to claim 5, characterized in that: A suction cup (8) is fixedly connected to the lower end of the support frame (2).

3. The organ-on-a-chip platform for simulating multi-organ interactions according to claim 2, characterized in that: The support frame (2) includes a frame (21), and a connecting column (22) is fixedly connected to the corner of the frame (21). A fixing hole (23) is opened at the lower end of the connecting column (22), and the upper end of the suction cup (8) is fixedly installed in the fixing hole (23).

4. The organ-on-a-chip platform for simulating multi-organ interactions according to claim 3, characterized in that: The guide frame (3) includes a first guide rod (31) and a second guide rod (32). The first guide rod (31) and the second guide rod (32) are each provided in pairs. The side end of the first guide rod (31) is fixedly connected to the side end of the second guide rod (32). The lower ends of the first guide rod (31) and the second guide rod (32) are fixedly connected to the upper ends of the frame (21) and the connecting column (22).

5. The organ-on-a-chip platform for simulating multi-organ interactions according to claim 4, characterized in that: The first movable guide bar (4) includes a first bar body (41), and a first sliding sleeve (42) is fixedly connected to the side end of the first bar body (41). The first sliding sleeve (42) is slidably sleeved on the first guide rod (31).

6. The organ-on-a-chip platform for simulating multi-organ interactions according to claim 4, characterized in that: The second movable guide bar (5) includes a second bar body (51), and a second sliding sleeve (52) is fixedly connected to the side end of the second bar body (51). The second sliding sleeve (52) is slidably mounted on the second guide rod (32).

7. The organ-on-a-chip platform for simulating multi-organ interactions according to claim 6, characterized in that: The movable support base (6) includes a base body (61), which is slidably installed outside the first body (41) and the second body (51). A collar (64) is fixedly connected to the side end of the base body (61), and a connecting piece (62) is fixedly connected to the side end of the base body (61). A pad block (63) is fixedly connected to the connecting piece (62).

8. The organ-on-a-chip platform for simulating multi-organ interactions according to claim 7, characterized in that: The bulb syringe (7) includes a glass tube (71) inserted into a collar (64), a retaining ring (72) fixedly connected to the middle of the glass tube (71), the retaining ring (72) blocking the collar (64), and an elastic airbag (73) fixedly connected to the upper end of the glass tube (71).

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