Preparation method of antibacterial skin-moistening mutton fat soap containing Artemisia stelleriana and Xanthoceras sorbifolia Bunge essential oil

Abstract

The invention belongs to the technical field of special perfumed soap, and discloses a preparation method of antibacterial skin-moistening mutton fat soap containing Artemisia stelleriana and Xanthoceras sorbifolia Bunge essential oil, and the preparation method comprises the following steps: S1, cutting mutton fat soap base into small blocks, and heating until the mutton fat soap base is completely melted; s2, adding the Artemisia stelleriana bacteriostatic microcapsules into deionized water, and stirring to form a uniform suspension; s3, after the temperature of the soap liquid is reduced to 45 DEG C, sequentially adding a composite humectant, hydrolyzed oat protein, a honeysuckle flower extract, a natural pigment, xanthoceras sorbifolia bunge essential oil liposome and an artemisia stelleriana antibacterial microcapsule suspension, and uniformly stirring; s4, pouring into a silica gel mold, demolding after cooling and curing, wiping the surface, standing for 2-3 weeks, and packaging; slow-release bacteriostasis of the Artemisia stelleriana bacteriostatic microcapsules and long-acting moisturizing of the Xanthoceras sorbifolia Bunge essential oil liposome form synergy, so that the product has the comprehensive advantages of strong bacteriostasis, high moisturizing and long-term stability, and the problems of insufficient bacteriostasis and poor moisturizing property of traditional soap-based soap are solved.

Description

Technical Field

[0001] This invention belongs to the field of specialty soap technology, specifically relating to a method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oils. Background Technology

[0002] The development trend of personal care products is evolving towards natural, gentle, and multifunctional products. Essential oil soaps, with their combined cleansing, fragrance, and skincare benefits, have become one of the mainstream products in the market. Essential oil soaps are essentially made by saponifying oils (animal, vegetable, or synthetic oils) with a strong alkali (such as sodium hydroxide) to convert them into soap (fatty acid salts) and glycerin. Glycerin is a natural moisturizer that helps retain skin moisture, which is a key reason why essential oil soaps are gentler than ordinary soaps. The soap base melting method for preparing essential oil soaps is widely used in home DIY and small-batch production due to its advantages of not requiring the treatment of a strong alkali, simple operation, and short processing time (1-2 hours). Its core principle is to heat and melt the existing saponification product (sodium fatty acid soap base), then add essential oils, natural extracts, and other functional ingredients to quickly form the finished soap. However, because the soap base loses some glycerin, the finished product's moisturizing effect is slightly inferior to that of the cold process method.

[0003] To enhance the antibacterial and moisturizing properties of essential oil soaps, existing technologies often incorporate natural plant extracts and essential oils. Artemisia annua, a traditional medicinal plant, contains flavonoids and terpenoids with significant antibacterial and anti-inflammatory activities, making it an ideal natural antibacterial ingredient. Xanthoceras sorbifolium essential oil is rich in unsaturated fatty acids (linoleic acid, oleic acid) and vitamin E, offering excellent moisturizing and nourishing effects, and can improve skin dryness after soap-based cleansing. However, Artemisia annua extract has low antibacterial activity, poor stability, and limited functionality, while Xanthoceras sorbifolium essential oil has poor compatibility, low moisturizing efficiency, and insufficient stability. Therefore, there is an urgent need to develop an antibacterial and moisturizing lanolin soap that combines natural plant extracts and essential oils to meet the pressing needs of people with dry and sensitive skin for gentle antibacterial and long-lasting moisturizing products. Summary of the Invention

[0004] To address the shortcomings mentioned in the background art, the present invention aims to provide a method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil. By combining the slow-release antibacterial properties of Artemisia annua microcapsules with the long-lasting moisturizing effect of Xanthoceras sorbifolium essential oil liposomes, the product simultaneously possesses the comprehensive advantages of strong antibacterial properties, high moisturizing properties, and long-lasting stability, thus solving the problems of insufficient antibacterial properties and poor moisturizing properties of traditional soap base soaps.

[0005] The objective of this invention can be achieved through the following technical solutions: A method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oils includes the following steps: S1. Cut the lanolin-based soap base into small pieces, heat it in a water bath over low heat until completely melted, stir for 20-40 seconds to remove air bubbles, and keep the temperature at 55℃ for later use. S2. Add the Artemisia annua antibacterial microcapsules to deionized water at 45℃ and stir for 40-60 seconds to form a uniform suspension; S3. Once the soap solution temperature drops to 45℃, add the compound moisturizer, hydrolyzed oat protein, honeysuckle extract, natural pigment, and liposomes of *Vernicia fordii* essential oil and suspension of *Artemisia argyi* antibacterial microcapsule suspension in sequence, and stir until completely homogeneous. S4. Pour into the silicone mold and gently vibrate to release air. Cool at room temperature for 1-2 hours. Once the soap has completely solidified, unmold it. After unmolding, wipe the surface with a dry cloth and place it in a cool, ventilated place for 2-3 weeks. Pack it in a sealed bag and moisture-proof paper. Artemisia annua antibacterial microcapsules are formed by encapsulating Artemisia annua enzymatic hydrolysate with β-cyclodextrin and gum arabic, and simultaneously loading zinc gluconate; Xanthoceras sorbifolium essential oil liposomes are prepared by low-temperature catalytic hydrogenation and then using soybean lecithin and cholesterol through thin-film dispersion method, while simultaneously loading ceramide.

[0006] Preferably, the antibacterial and moisturizing lanolin soap contains the following raw materials in parts by weight: 85-88 parts lanolin-based soap base, 1.5-2.5 parts Artemisia annua antibacterial microcapsules, 1.0-1.8 parts Xanthoceras sorbifolium essential oil liposomes, 4-6 parts compound moisturizer, 0.3-0.7 parts honeysuckle extract, 1.0-1.5 parts hydrolyzed oat protein, and 0.05-0.1 parts natural pigment.

[0007] Preferably, the preparation method of Artemisia annua antibacterial microcapsules includes the following steps: A. Weigh β-cyclodextrin and gum arabic, add them to deionized water, and stir in a 60°C water bath until completely dissolved to obtain a wall material solution; B. Add zinc gluconate to the concentrated enzyme hydrolysis solution of Artemisia annua and stir until completely dissolved to obtain the core material solution; C. Slowly pour the core material solution into the wall material solution, stir at 40℃ and 200r / min for 40-60min to form a stable mixture, and dry the mixture by spray dryer to obtain microcapsule powder; D. The dried microcapsule powder was frozen at -18℃ for 1.5 to 2.5 hours, then left at room temperature for 10 to 14 hours, and passed through a 100-mesh sieve to obtain Artemisia annua antibacterial microcapsules.

[0008] Preferably, the solid content of the Artemisia annua enzymatic hydrolysis concentrate is ≥30%, and the preparation method of the Artemisia annua enzymatic hydrolysis concentrate includes the following steps: a. Crush the dried whole plant of Artemisia annua through an 80-mesh sieve using a high-speed grinder. Take the fine powder of Artemisia annua and add it to deionized water. Stir well to form a suspension. Then adjust the pH to 5.0 with hydrochloric acid and preheat in a 45℃ constant temperature water bath for 20-40 minutes. b. Weigh out 0.25% of cellulase and pectinase from Artemisia annua powder and add them to deionized water. Stir to dissolve and slowly add the enzyme solution to the preheated Artemisia annua suspension. In a constant temperature water bath at 45°C, stir and react for 1-2 hours. c. Heat the suspension to 90℃ and keep it at that temperature for 15-25 minutes. Filter the solution while it is hot and collect the filtrate. Place the filtrate in a rotary evaporator and concentrate it at 60℃ and -0.08MPa until the solid content is ≥30% to obtain the concentrated enzymatic hydrolysate of Artemisia annua.

[0009] Preferably, the mass ratio of β-cyclodextrin, gum arabic, concentrated enzymatic hydrolysate of Artemisia annua, and zinc gluconate is 3:1:6:0.01.

[0010] Preferably, the spray dryer has an inlet air temperature of 160°C, an outlet air temperature of 80°C, a feed rate of 5 mL / min, and an atomization pressure of 0.3 MPa.

[0011] Preferably, the method for preparing *Sapindus mukorossi* essential oil liposomes includes the following steps: (1) Add refined Sapindus mukorossi essential oil to a high-pressure reactor, then add 0.1% palladium on carbon catalyst by weight of essential oil, introduce hydrogen into the reactor, stir and react at 0.3 MPa and 50°C for 1-3 hours, cool to room temperature after reaction, filter to remove palladium on carbon catalyst, collect hydrogenated essential oil, purify by silica gel column chromatography, collect target fraction, and remove eluent by rotary evaporation; (2) Add soybean lecithin and cholesterol to anhydrous ethanol, stir in a 60°C water bath until completely dissolved to obtain a membrane material solution. Take hydrogenated Xanthoceras sorbifolium essential oil, add ceramide, stir for 20 minutes until completely dissolved to obtain a core material solution. (3) Mix the membrane material solution and the core material solution, remove ethanol by rotary evaporation at 50℃ and -0.08MPa, add deionized water, and continue rotary evaporation for 10-20 minutes to fully hydrate the lipid membrane and form a crude liposome suspension. (4) Pour the crude liposome suspension into an ultrasonic cell disruptor and sonicate at 300W for 5-15 minutes to refine the particle size. Then centrifuge the sonicated suspension for 10-20 minutes and collect the supernatant to obtain the liposomes of Sapindus mukorossi essential oil.

[0012] Preferably, the mass ratio of hydrogenated *Sapindus mukorossi* essential oil, soybean lecithin, cholesterol, and ceramide is 10:7:3:0.03.

[0013] Preferably, the compound moisturizer is a mixture of glycerin, lanolin and sodium hyaluronate in a mass ratio of 5:4:1.

[0014] Preferably, the natural pigment is one or a mixture of several of safflower, indigo and turmeric.

[0015] The beneficial effects of this invention are as follows: This invention prepares antibacterial and moisturizing lanolin soap using a soap base melting method. Ready-made lanolin soap base is heated and melted, and then ingredients such as Artemisia annua antibacterial microcapsules, Xanthoceras sorbifolium essential oil liposomes, a complex moisturizer, honeysuckle extract, and hydrolyzed oat protein are added. This method is simple and quick. Lanolin in the soap base is rich in unsaturated fatty acids, enhancing the soap's moisturizing properties and reducing irritation; sodium fatty acids provide basic cleansing power and shape retention. The Artemisia annua antibacterial microcapsules contain natural antibacterial active ingredients (including flavonoids and terpenoids) that inhibit Staphylococcus aureus and Escherichia coli, while also soothing inflammation and repairing the skin barrier. Xanthoceras sorbifolium essential oil liposomes are rich in unsaturated fatty acids (linoleic acid and oleic acid) and vitamin E, deeply moisturizing and locking in moisture, improving dryness and tightness after cleansing, and leaving a delicate and firm finish. Fruity aroma; the compound moisturizer contains glycerin for rapid hydration, lanolin and sheep fat base to work synergistically to form a breathable moisturizing film, enhancing the lasting moisturizing effect, sodium hyaluronate to penetrate the stratum corneum and enhance water-locking ability, forming a closed loop of hydration, water-locking and skin-nourishing with the essential oil of Xanthoceras sorbifolium; honeysuckle extract and artemisia annua extract work synergistically to enhance broad-spectrum antibacterial properties and extend the shelf life of the soap; oat extract (hydrolyzed oat protein) soothes and calms, reduces the irritation of the soap base to sensitive skin, improves skin compatibility, and reduces dryness and flaking.

[0016] This invention relates to *Artemisia annua* antibacterial microcapsules, which encapsulate enzymatically hydrolyzed *Artemisia annua* extract with β-cyclodextrin and gum arabic, and simultaneously load zinc gluconate. The combined enzymatic hydrolysis of cellulase and pectinase disrupts the cell wall structure of *Artemisia annua*, increasing the dissolution rate of antibacterial active ingredients (flavonoids, terpenes) and forming smaller molecular structures that are more easily encapsulated, thus solving the problem of insufficient release of active ingredients from traditional extracts. The wall material formed by β-cyclodextrin and gum arabic isolates the antibacterial ingredients from the soap solution, preventing damage to the active ingredients; during use, skin friction pressure triggers the wall material to rupture, allowing for the slow release of antibacterial ingredients and prolonging the antibacterial effect. The zinc ions loaded during microcapsule preparation form a synergistic system of plant active ingredients and metal ions with the *Artemisia annua* extract. Zinc ions disrupt bacterial cell membrane permeability, promoting the entry of flavonoids into the bacteria to inhibit metabolism, thereby adding a targeted antibacterial function against *Propionibacterium acnes*.

[0017] This invention relates to *Xanthoceras sorbifolium* essential oil liposomes prepared via low-temperature catalytic hydrogenation using soybean lecithin and cholesterol through a thin-film dispersion method, while simultaneously loading ceramides. Low-temperature catalytic hydrogenation converts some unsaturated fatty acids in the *Xanthoceras sorbifolium* essential oil into more stable monounsaturated fatty acids, lowers the iodine value, prevents oxidative rancidity, improves the high-temperature stability of the soap, and retains moisturizing activity. The liposomes formed from soybean lecithin and cholesterol have a structure similar to the phospholipid bilayer of the stratum corneum of the skin. Through fusion and penetration, they deliver unsaturated fatty acids, vitamin E, and other nutrients from the essential oil into the stratum corneum of the skin, rather than remaining on the surface, thereby improving the moisturizing rate. The liposome membrane encapsulates the essential oil to form a closed system, preventing evaporation, while the liposomes slowly release essential oil molecules, resulting in a more lasting and gentle fragrance.

[0018] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0020] Example 1

[0021] An antibacterial microcapsule for Artemisia annua, comprising microcapsules formed by encapsulating an enzymatically hydrolyzed extract of Artemisia annua with β-cyclodextrin and gum arabic, and simultaneously loading zinc gluconate, is prepared by the following steps: A. Weigh 15g of β-cyclodextrin and 5g of gum arabic, add 100mL of deionized water, and stir in a 60℃ water bath until completely dissolved to obtain a wall material solution; B. Take 0.06g of zinc gluconate and add it to 30g of Artemisia annua enzymatic hydrolysis concentrate. Stir until completely dissolved to obtain the core material solution. C. Slowly pour the core material solution into the wall material solution, stir at 40℃ and 200r / min for 50min to form a stable mixture, add the mixture to a spray dryer with an inlet air temperature of 160℃, an outlet air temperature of 80℃, a feed rate of 5mL / min, and an atomization pressure of 0.3MPa, and dry the mixture in the spray dryer to obtain microcapsule powder; D. The dried microcapsule powder was frozen at -18℃ for 2 hours, then left at room temperature for 12 hours, and passed through a 100-mesh sieve to obtain the Artemisia annua antibacterial microcapsules.

[0022] The solid content of the above-mentioned Artemisia annua enzymatic hydrolysis concentrate is ≥30%. The preparation method of the Artemisia annua enzymatic hydrolysis concentrate includes the following steps: a. Crush the dried whole plant of Artemisia annua using a high-speed grinder and pass it through an 80-mesh sieve. Take 100g of Artemisia annua powder and add it to 500mL of deionized water. Stir well to form a suspension. Then adjust the pH to 5.0 with 1mol / L hydrochloric acid and preheat in a 45℃ constant temperature water bath for 30min. b. Weigh 0.25g of cellulase and 0.25g of pectinase respectively, add them to 50mL of deionized water and stir to dissolve. Slowly add the enzyme solution to the preheated Artemisia annua suspension, and stir in a 45℃ water bath for 1.5h. c. Heat the suspension to 90℃ and keep it at that temperature for 20 minutes. Filter the solution while it is still hot and collect the filtrate. Place the filtrate in a rotary evaporator and concentrate it at 60℃ and -0.08MPa until the solid content is ≥30% to obtain the concentrated enzymatic hydrolysate of Artemisia annua.

[0023] Example 2

[0024] A type of *Xanthoceras sorbifolium* essential oil liposome, prepared by low-temperature catalytic hydrogenation using soybean lecithin and cholesterol via thin-film dispersion, and simultaneously loaded with ceramide, comprises the following steps: (1) Add 100g of refined Sapindus mukorossi essential oil to a high-pressure reactor, then add 0.1g of palladium on carbon catalyst, introduce hydrogen into the reactor, stir and react for 2h at 0.3MPa and 50℃, cool to room temperature after the reaction, filter to remove palladium on carbon catalyst, collect the hydrogenated essential oil, purify by silica gel column chromatography (silica gel particle size 100-200 mesh, eluent is n-hexane: ethanol = 9:1), collect the target fraction, and remove the eluent by rotary evaporation; (2) Add 7g of soybean lecithin and 3g of cholesterol to 50mL of anhydrous ethanol, stir in a 60℃ water bath until completely dissolved to obtain a membrane material solution. Take 10g of hydrogenated Xanthoceras sorbifolium essential oil, add 0.03g of ceramide, stir for 20min until completely dissolved to obtain a core material solution. (3) Mix the membrane material solution and the core material solution, remove the ethanol by rotary evaporation at 50℃ and -0.08MPa, add 100mL of deionized water, and continue rotary evaporation for 15min to fully hydrate the lipid membrane and form a crude liposome suspension. (4) Pour the crude liposome suspension into an ultrasonic cell disruptor and sonicate at 300W for 10 min to refine the particle size. Then centrifuge the sonicated suspension for 15 min and collect the supernatant to obtain the liposomes of Sapindus mukorossi essential oil.

[0025] Example 3

[0026] A method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oils includes the following steps: S1. Cut the lanolin-based soap base into small pieces, heat it in a water bath over low heat to 60°C until completely melted, stir for 20 seconds to remove air bubbles, and keep the temperature at 55°C for later use. S2. Add the Artemisia annua antibacterial microcapsules to deionized water at 45℃ and stir for 60 seconds to form a uniform suspension; S3. Once the soap solution temperature drops to 45℃, add the compound moisturizer, hydrolyzed oat protein, honeysuckle extract, natural pigment, and liposomes of *Vernicia fordii* essential oil and suspension of *Artemisia argyi* antibacterial microcapsule suspension in sequence, and stir until completely homogeneous. S4. Pour into the silicone mold and gently vibrate to release air. Cool at room temperature for 1 hour. Once the soap has completely solidified, unmold it. After unmolding, wipe the surface with a dry cloth and place it in a cool, ventilated place for 2-3 weeks. Pack it in a sealed bag and moisture-proof paper.

[0027] The above-mentioned antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil comprises the following raw materials in parts by weight: 88 parts lanolin-based soap base, 1.5 parts Artemisia annua antibacterial microcapsules, 1.8 parts Xanthoceras sorbifolium essential oil liposomes, 2 parts glycerin, 1.6 parts lanolin, 0.4 parts sodium hyaluronate, 0.7 parts honeysuckle extract, 1.0 part hydrolyzed oat protein, and 0.1 parts natural pigment safflower; the Artemisia annua antibacterial microcapsules were prepared in Example 1; and the Xanthoceras sorbifolium essential oil liposomes were prepared in Example 2.

[0028] Example 4

[0029] A method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oils includes the following steps: S1. Cut the lanolin-based soap base into small pieces, heat it in a water bath over low heat to 60°C until completely melted, stir for 40 seconds to remove air bubbles, and keep the temperature at 55°C for later use. S2. Add the Artemisia annua antibacterial microcapsules to deionized water at 45℃ and stir for 40 seconds to form a uniform suspension; S3. Once the soap solution temperature drops to 45℃, add the compound moisturizer, hydrolyzed oat protein, honeysuckle extract, natural pigment, and liposomes of *Vernicia fordii* essential oil and suspension of *Artemisia argyi* antibacterial microcapsule suspension in sequence, and stir until completely homogeneous. S4. Pour into the silicone mold and gently vibrate to release air. Cool at room temperature for 2 hours. Once the soap has completely solidified, unmold it. After unmolding, wipe the surface with a dry cloth and place it in a cool, ventilated place for 2-3 weeks. Pack it in a sealed bag and moisture-proof paper.

[0030] The above-mentioned antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil comprises the following raw materials in parts by weight: 85 parts lanolin-based soap base, 2.5 parts Artemisia annua antibacterial microcapsules, 1.0 part Xanthoceras sorbifolium essential oil liposomes, 3 parts glycerin, 2.4 parts lanolin, 0.6 parts sodium hyaluronate, 0.3 parts honeysuckle extract, 1.5 parts hydrolyzed oat protein, and 0.05 parts natural pigment Polygonum indicum; the Artemisia annua antibacterial microcapsules were prepared in Example 1; and the Xanthoceras sorbifolium essential oil liposomes were prepared in Example 2.

[0031] Example 5

[0032] A method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oils includes the following steps: S1. Cut the lanolin-based soap base into small pieces, heat it in a water bath over low heat to 60°C until completely melted, stir for 30 seconds to remove air bubbles, and keep the temperature at 55°C for later use. S2. Add the Artemisia annua antibacterial microcapsules to deionized water at 45℃ and stir for 50 seconds to form a uniform suspension; S3. Once the soap solution temperature drops to 45℃, add the compound moisturizer, hydrolyzed oat protein, honeysuckle extract, natural pigment, and liposomes of *Vernicia fordii* essential oil and suspension of *Artemisia argyi* antibacterial microcapsule suspension in sequence, and stir until completely homogeneous. S4. Pour into the silicone mold and gently vibrate to release air. Cool at room temperature for 1.5 hours. Once the soap has completely solidified, unmold it. After unmolding, wipe the surface with a dry cloth and place it in a cool, ventilated place for 2-3 weeks. Pack it in a sealed bag and moisture-proof paper.

[0033] The above-mentioned antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil comprises the following raw materials in parts by weight: 86.5 parts lanolin-based soap base, 2 parts Artemisia annua antibacterial microcapsules, 1.4 parts Xanthoceras sorbifolium essential oil liposomes, 2.5 parts glycerin, 2 parts lanolin, 0.5 parts sodium hyaluronate, 0.5 parts honeysuckle extract, 1.2 parts hydrolyzed oat protein, and 0.08 parts natural pigment turmeric; the Artemisia annua antibacterial microcapsules were prepared in Example 1; and the Xanthoceras sorbifolium essential oil liposomes were prepared in Example 2.

[0034] Comparative Example 1 A method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oils includes the following steps: S1. Cut the lanolin-based soap base into small pieces, heat it in a water bath over low heat to 60°C until completely melted, stir for 30 seconds to remove air bubbles, and keep the temperature at 55°C for later use. S2. Once the soap solution temperature drops to 45℃, add the compound moisturizer, hydrolyzed oat protein, honeysuckle extract, natural pigment, and liposomes of *Sapindus mukorossi* essential oil and *Artemisia argyi* extract in sequence, and stir until completely homogeneous. S3. Pour into the silicone mold and gently vibrate to release air. Cool at room temperature for 1.5 hours. Once the soap has completely solidified, unmold it. After unmolding, wipe the surface with a dry cloth and place it in a cool, ventilated place for 2-3 weeks. Pack it in a sealed bag and moisture-proof paper.

[0035] The above-mentioned antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil comprises the following raw materials in parts by weight: 86.5 parts lanolin-based soap base, 2 parts Artemisia annua extract, 1.4 parts Xanthoceras sorbifolium essential oil liposomes, 2.5 parts glycerin, 2 parts lanolin, 0.5 parts sodium hyaluronate, 0.5 parts honeysuckle extract, 1.2 parts hydrolyzed oat protein, and 0.08 parts natural pigment turmeric; the Xanthoceras sorbifolium essential oil liposomes were prepared in Example 2.

[0036] Comparative Example 2 A method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oils includes the following steps: S1. Cut the lanolin-based soap base into small pieces, heat it in a water bath over low heat to 60°C until completely melted, stir for 30 seconds to remove air bubbles, and keep the temperature at 55°C for later use. S2. Add the antibacterial microcapsules of Artemisia annua to deionized water at 45℃ and stir for 40-60 seconds to form a uniform suspension. Mix the refined Sapindus mukorossi essential oil with Tween 80 and stir until a uniform emulsion is formed. S3. Once the soap solution temperature drops to 45℃, add glycerin, lanolin, sodium hyaluronate, hydrolyzed oat protein, honeysuckle extract, natural pigment turmeric, and the pre-emulsified *Vernicia fordii* essential oil and *Artemisia argyi* antibacterial microcapsule mixture in sequence, and stir until completely homogeneous. S4. Pour into the silicone mold and gently vibrate to release air. Cool at room temperature for 1.5 hours. Once the soap has completely solidified, unmold it. After unmolding, wipe the surface with a dry cloth and place it in a cool, ventilated place for 2-3 weeks. Pack it in a sealed bag and moisture-proof paper.

[0037] The above-mentioned antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil comprises the following raw materials in parts by weight: 86.5 parts lanolin-based soap base, 2 parts Artemisia annua antibacterial microcapsules, 1.4 parts Xanthoceras sorbifolium essential oil, 2.5 parts glycerin, 2 parts lanolin, 0.5 parts sodium hyaluronate, 0.5 parts honeysuckle extract, 1.2 parts hydrolyzed oat protein, and 0.08 parts natural pigment turmeric; the Artemisia annua antibacterial microcapsules were prepared in Example 1.

[0038] Performance testing The antibacterial and moisturizing lanolin soaps containing Artemisia annua and Xanthoceras sorbifolium essential oils prepared in Example 5, Comparative Example 1, and Comparative Example 2 were subjected to the following performance tests: (1) Antibacterial performance test: referring to the agar diffusion method in GB15979-2002 "Hygienic Standard for Disposable Sanitary Products", Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC25922), and Propionibacterium acnes (ATCC6919) were selected as test strains. 5.0g of sample was weighed, 45mL of sterile physiological saline was added, and the mixture was shaken for 30min to prepare a 1:10 sample dilution. After sterilization of LB agar medium, the solution was cooled to 45℃ and poured into petri dishes (90mm in diameter), 20mL per dish. After solidification, 0.1mL of bacterial solution (concentration 10) was taken for later use. 6 The sample dilution (CFU / mL) was evenly spread on the surface of the culture medium, and a sterile Oxford cup (6 mm inner diameter) was placed in it. 0.2 mL of sample dilution was injected and the sample was incubated at 37°C for 24 h. The diameter of the inhibition zone (mm) was measured with calipers. Each sample was tested in parallel 3 times and the average value was taken. The inhibition rate = (inhibition zone diameter - Oxford cup inner diameter) / Oxford cup inner diameter × 100%. The samples from Example 5, Comparative Example 1 and Comparative Example 2 were sealed and placed in an environment of 25°C and 60% humidity for 6 months. During this period, samples were taken once a month and the inhibition rate was tested according to the above steps. (2) Moisturizing performance test: referring to QB / T4256-2011 "Guidelines for Evaluation of Moisturizing Efficacy of Cosmetics", 10 healthy subjects (20-35 years old, 5 males and 5 females, skin types covering dry and normal) were selected. The test environment temperature was 22±1℃ and the humidity was 50±5%. The subjects adapted to the environment for 30 minutes. Three 2cm×2cm areas were marked on the inner side of the forearm (corresponding to 3 groups of samples respectively). The blank area was used as a control. The test area was cleaned with deionized water and dried. The initial skin moisture content (Corneometer CM825) was measured. 0.1g of sample was evenly applied to the test area and massaged until completely dissolved. The skin moisture content was measured at 10min, 30min and 60min after use. Moisturizing rate = (moisture content after use - initial moisture content) / initial moisture content × 100%. The average value of 10 subjects was taken. (3) Aroma retention rate detection: Gas chromatography-mass spectrometry (GC-MS) combined with sensory evaluation method was used. For GC-MS, after the sample was sealed and placed for 60 days, 5.0 g of sample was weighed, 10 mL of anhydrous ethanol was added, and ultrasonic extraction was performed for 30 min. After filtration, the supernatant was injected. Chromatographic column: HP-5MS (30 m × 0.25 mm × 0.25 μm), column temperature 40 ℃ (hold for 2 min) → 10 ℃ / min to 250 ℃ (hold for 5 min), carrier gas He, flow rate 1 mL / min; Sensory evaluation was performed by 5 professional evaluators who scored the aroma intensity (1-5 points) of the samples placed for 0 days, 30 days and 60 days. Aroma retention rate = (60-day score / 0-day score) × 100%; (4) Skin irritation test: According to the skin patch test in the 2015 edition of the Cosmetic Safety Technical Specifications, the sample was crushed and mixed with deionized water to make a 50% (w / w) suspension. 20 μL of the suspension was dropped onto the patch applicator (diameter 8 mm) and applied to the inner side of the subject's forearm. After 24 h, the patch applicator was removed and the skin reaction was observed at 24 h and 48 h. The positive rate was calculated according to the standard (0~4 points) and the positive rate was calculated as (number of people with irritation reaction / total number of people) × 100%. The results are shown in Table 1 below.

[0039] Table 1. Performance Test Results of Antibacterial and Moisturizing Lanolin Soap

[0040] As shown in Table 1, Example 5 exhibits a significantly higher antibacterial rate against Staphylococcus aureus and Escherichia coli compared to Comparative Example 1, and also demonstrates an antibacterial effect against Propionibacterium acnes, which Comparative Example 1 lacks. Furthermore, the antibacterial effect of Example 5 remained above 95% after a 6-month long-term storage period, while that of Comparative Example 1 decreased to below 55%. This indicates that the antibacterial components (flavonoids and terpenes) of the unmodified Artemisia annua extract, when directly exposed to soap solution, are easily destroyed by the weakly alkaline environment of the soap base and are rapidly lost during use, resulting in a low antibacterial rate and lack of long-lasting effect. In contrast, the modified Artemisia annua antibacterial microcapsules achieve a dual synergistic effect through enzymatic hydrolysis and microcapsule encapsulation, significantly improving antibacterial performance and stability.

[0041] Example 5 showed a 30-minute moisturizing rate that was more than 50% higher than that of Comparative Example 2, and the 60-minute moisturizing rate remained at 39.8%, indicating that the moisturizing durability of the modified *Xanthoceras sorbifolium* essential oil was significantly improved. Simultaneously, the aroma retention rate of Example 5 was more than double that of Comparative Example 2. It is evident that the unmodified *Xanthoceras sorbifolium* essential oil is fat-soluble and has poor compatibility with aqueous soap solutions. During application, it tends to float on the skin surface, making it difficult to penetrate and absorb. Furthermore, its unsaturated fatty acids are prone to oxidation and rancidity, leading to aroma loss and soap softening. In contrast, the modified *Xanthoceras sorbifolium* essential oil liposomes achieve triple optimization through double bond hydrogenation and liposome loading, enhancing moisturizing performance, aroma, and oxidative stability.

[0042] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0043] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A method for preparing an antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil, characterized in that, Includes the following steps: S1. Cut the lanolin-based soap base into small pieces, heat it in a water bath over low heat until completely melted, stir for 20-40 seconds to remove air bubbles, and keep the temperature at 55℃ for later use. S2. Add the Artemisia annua antibacterial microcapsules to deionized water at 45℃ and stir for 40-60 seconds to form a uniform suspension; S3. Once the soap solution temperature drops to 45℃, add the compound moisturizer, hydrolyzed oat protein, honeysuckle extract, natural pigment, and liposomes of *Vernicia fordii* essential oil and suspension of *Artemisia argyi* antibacterial microcapsule suspension in sequence, and stir until completely homogeneous. S4. Pour into the silicone mold and gently vibrate to release air. Cool at room temperature for 1-2 hours. Once the soap has completely solidified, unmold it. After unmolding, wipe the surface with a dry cloth and place it in a cool, ventilated place for 2-3 weeks. Pack it in a sealed bag and moisture-proof paper. The antibacterial microcapsules of Artemisia annua are formed by encapsulating the enzymatic hydrolysate of Artemisia annua with β-cyclodextrin and gum arabic, and are simultaneously loaded with zinc gluconate; the liposomes of Xanthoceras sorbifolium essential oil are prepared by thin-film dispersion of soybean lecithin and cholesterol after low-temperature catalytic hydrogenation, and are simultaneously loaded with ceramide.

2. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 1, characterized in that, The antibacterial and moisturizing lanolin soap contains the following ingredients in parts by weight: 85-88 parts lanolin-based soap base, 1.5-2.5 parts Artemisia annua antibacterial microcapsules, 1.0-1.8 parts Xanthoceras sorbifolium essential oil liposomes, 4-6 parts compound moisturizer, 0.3-0.7 parts honeysuckle extract, 1.0-1.5 parts hydrolyzed oat protein, and 0.05-0.1 parts natural pigment.

3. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 1, characterized in that, The preparation method of the Artemisia annua antibacterial microcapsules includes the following steps: A. Weigh β-cyclodextrin and gum arabic, add them to deionized water, and stir in a 60°C water bath until completely dissolved to obtain a wall material solution; B. Add zinc gluconate to the concentrated enzyme hydrolysis solution of Artemisia annua and stir until completely dissolved to obtain the core material solution; C. Slowly pour the core material solution into the wall material solution, stir at 40℃ and 200r / min for 40-60min to form a stable mixture, and dry the mixture by spray dryer to obtain microcapsule powder; D. The dried microcapsule powder was frozen at -18℃ for 1.5 to 2.5 hours, then left at room temperature for 10 to 14 hours, and passed through a 100-mesh sieve to obtain the Artemisia annua antibacterial microcapsules.

4. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 3, characterized in that, The solid content of the Artemisia annua enzymatic hydrolysis concentrate is ≥30%, and the preparation method of the Artemisia annua enzymatic hydrolysis concentrate includes the following steps: a. Crush the dried whole plant of Artemisia annua through an 80-mesh sieve using a high-speed grinder. Take the fine powder of Artemisia annua and add it to deionized water. Stir well to form a suspension. Then adjust the pH to 5.0 with hydrochloric acid and preheat in a 45℃ constant temperature water bath for 20-40 minutes. b. Weigh out 0.25% of cellulase and pectinase from Artemisia annua powder and add them to deionized water. Stir to dissolve and slowly add the enzyme solution to the preheated Artemisia annua suspension. In a constant temperature water bath at 45°C, stir and react for 1-2 hours. c. Heat the suspension to 90℃ and keep it at that temperature for 15-25 minutes. Filter the solution while it is hot and collect the filtrate. Place the filtrate in a rotary evaporator and concentrate it at 60℃ and -0.08MPa until the solid content is ≥30% to obtain the concentrated enzymatic hydrolysate of Artemisia annua.

5. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 3, characterized in that, The mass ratio of β-cyclodextrin, gum arabic, Artemisia annua enzymatic hydrolysis concentrate, and zinc gluconate is 3:1:6:0.

01.

6. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 3, characterized in that, The spray dryer has an inlet air temperature of 160℃, an outlet air temperature of 80℃, a feed rate of 5mL / min, and an atomization pressure of 0.3MPa.

7. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 1, characterized in that, The method for preparing the *Sapindus mukorossi* essential oil liposomes includes the following steps: (1) Add refined Sapindus mukorossi essential oil to a high-pressure reactor, then add 0.1% palladium on carbon catalyst by weight of essential oil, introduce hydrogen into the reactor, stir and react at 0.3 MPa and 50°C for 1-3 hours, cool to room temperature after reaction, filter to remove palladium on carbon catalyst, collect hydrogenated essential oil, purify by silica gel column chromatography, collect target fraction, and remove eluent by rotary evaporation; (2) Add soybean lecithin and cholesterol to anhydrous ethanol, stir in a 60°C water bath until completely dissolved to obtain a membrane material solution. Take hydrogenated Xanthoceras sorbifolium essential oil, add ceramide, stir for 20 minutes until completely dissolved to obtain a core material solution. (3) Mix the membrane material solution and the core material solution, remove ethanol by rotary evaporation at 50℃ and -0.08MPa, add deionized water, and continue rotary evaporation for 10-20 minutes to fully hydrate the lipid membrane and form a crude liposome suspension. (4) Pour the crude liposome suspension into an ultrasonic cell disruptor and sonicate at 300W for 5-15 minutes to refine the particle size. Then centrifuge the sonicated suspension for 10-20 minutes and collect the supernatant to obtain the liposomes of Sapindus mukorossi essential oil.

8. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 5, characterized in that, The mass ratio of the hydrogenated *Sapindus mukorossi* essential oil, soybean lecithin, cholesterol, and ceramide is 10:7:3:0.

03.

9. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 1, characterized in that, The compound moisturizer is a mixture of glycerin, lanolin, and sodium hyaluronate in a mass ratio of 5:4:

1.

10. The method for preparing the antibacterial and moisturizing lanolin soap containing Artemisia annua and Xanthoceras sorbifolium essential oil according to claim 1, characterized in that, The natural pigment is one or a mixture of several of safflower, indigo and turmeric.

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