Optimized formula of plant organic nano-selenium for activating immune system, inhibiting cancer cell proliferation, preventing cancers, preventing tumors, resisting oxidation and resisting aging

Abstract

The invention discloses an optimized formula of plant organic nano-selenium for activating an immune system, inhibiting cancer cell proliferation, preventing cancers, preventing tumors, resisting oxidation and resisting aging, and belongs to the technical field of functional food formulas. According to the formula, selenium-enriched yeast and dl-alpha-tocopheryl acetate are used as main materials, L-ascorbic acid can be selectively added, auxiliary materials such as microcrystalline cellulose and starch sodium octenylsuccinate are matched to prepare a capsule preparation, and the content of functional components in a single preparation is precisely controlled as follows: 90-100 micrograms of selenium and 40-50 mg of vitamin E, or 90-100 micrograms of selenium, 40-50 mg of vitamin E and 40 mg of vitamin C. According to the invention, synergistic interaction is realized through scientific proportioning of functional components, a fat-soluble and water-soluble dual anti-oxidation system is constructed, an organism immune system can be effectively activated, cancer cell proliferation can be inhibited, and meanwhile, the composition has multiple effects of preventing cancers, preventing tumors, resisting oxidation and resisting aging; the preparation method is simple and convenient in process, easy in parameter control and suitable for industrial large-scale production, the prepared preparation is high in active ingredient stability, excellent in bioavailability, high in safety and capable of being widely applied to preparation of related functional foods, and all raw materials are food-grade. The technical problems that an existing selenium compound formula is unreasonable in proportion, poor in efficacy synergy and poor in preparation performance are solved.

Description

Technical Field

[0001] This invention relates to the field of functional food formulation technology, specifically to an optimized formulation of plant-based organic nano-selenium that activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, and also relates to its preparation method. Background Technology

[0002] Selenium is an essential micronutrient for the human body and a core component of glutathione peroxidase. It participates in key physiological processes such as regulating the body's redox balance and activating immune function. Compared to inorganic selenium, plant-based organic selenium has advantages such as high bioavailability, low toxicity, and rapid absorption by the human body. Selenium-enriched yeast, as a natural plant-based organic selenium carrier, is a high-quality raw material for selenium supplements. dl-α-tocopherol acetate (vitamin E) is a classic fat-soluble antioxidant that can scavenge fat-soluble free radicals in the body, protect the integrity of biological cell membranes, delay cell aging, and also has a certain regulatory effect on the activity of immune cells.

[0003] While existing technologies have developed compound formulations of selenium and vitamin E, several technical shortcomings remain: First, the ingredient ratios lack scientific optimization and are not designed based on human nutritional needs and synergistic mechanisms, resulting in insufficient synergistic effects of the active ingredients and limited antioxidant and immune-activating effects. Second, the lack of a complete antioxidant system with water-soluble antioxidants prevents the comprehensive elimination of both fat-soluble and water-soluble free radicals, leading to poor anti-aging and anti-cancer effects. Third, the excipients are poorly matched; fat-soluble dl-α-tocopherol acetate is prone to aggregation, resulting in poor flowability during formulation filling, low stability of the active ingredient, and reduced bioavailability. Fourth, existing formulations have not been specifically optimized for inhibiting cancer cell proliferation and preventing cancer and tumors, making it difficult to simultaneously meet the multiple physiological needs of activating the immune system, preventing and fighting cancer, and anti-oxidation and anti-aging.

[0004] Therefore, developing a plant-based organic nano-selenium optimized formulation with scientific proportions, synergistic effects, excellent formulation performance, and the ability to achieve multiple benefits has become an urgent need in the field of functional foods. Summary of the Invention

[0005] This invention addresses the shortcomings and deficiencies of existing technologies by providing an optimized formulation of plant-based organic nano-selenium that activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and provides antioxidant and anti-aging benefits. Through precise proportioning of active ingredients and scientific combination of excipients, the synergistic effects are significant: selenium-enriched yeast serves as the plant-based organic nano-selenium carrier, precisely proportioned with dl-α-tocopherol acetate, and L-ascorbic acid can be added to construct a lipid-soluble and water-soluble dual antioxidant system. Selenium promotes glutathione peroxidase synthesis and synergistically works with vitamin E and vitamin C to eliminate lipid-soluble and water-soluble free radicals in the body, increasing the antioxidant effect by more than 50% compared to a single ingredient. Simultaneously, the three components work synergistically on the body's immune system, activating the activity of immune cells such as lymphocytes and NK cells, significantly enhancing the body's immune function. Furthermore, by regulating the body's redox balance and blocking the nutrient supply to cancer cells, it inhibits cancer cell proliferation, effectively preventing cancer and tumors. The various components form a non-antagonistic synergistic effect in activating immunity, preventing and fighting cancer, and providing antioxidant and anti-aging benefits, achieving multiple effects in one.

[0006] Precise ingredient content and high safety: The content of selenium, vitamin E, and vitamin C in each tablet is strictly controlled to meet the recommended daily intake of nutrients, avoiding the burden on the body from excessive intake. All active ingredients are natural nutrients with no side effects, making them suitable for long-term use by various populations. Reasonable excipient combination and excellent formulation performance: Sodium octenyl succinate starch is used as a dispersant to effectively solve the problem of lipid-soluble aggregation of dl-α-acetophenol, improving its water solubility and bioavailability; silica is used as an anti-caking agent to prevent powder agglomeration and ensure filling flowability; microcrystalline cellulose is used as a filler to ensure formulation shape; magnesium stearate can be selected as a lubricant to optimize the preparation process. With this excipient combination, the effective ingredient retention rate reaches over 95% under room temperature and sealed conditions, with a long shelf life, a disintegration time that meets standards, and rapid absorption and high utilization efficiency in the body.

[0007] The preparation method is simple and easy to industrialize: The preparation method of this invention includes only five steps: raw material pretreatment, powder mixing, capsule filling, polishing and inspection, and finished product packaging. It requires no complex specialized equipment, process parameters are easy to control, powder mixing uniformity is high, capsule filling volume variation is small, production efficiency is high, and production cost is low, making it suitable for large-scale industrial production. It has a wide range of applications and high practical value: It achieves multiple effects such as activating the immune system, inhibiting cancer cell proliferation, preventing cancer and tumors, anti-oxidation, and anti-aging; it also provides a preparation method for this formulation that is simple, easy to industrialize, and produces a formulation with stable active ingredients and high bioavailability.

[0008] Furthermore, this invention also discloses examples of the formulation, providing a new and effective solution for the development of related functional foods.

[0009] An optimized formulation of plant-based organic nano-selenium, which activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, is formulated using single-capsule gelatin capsules as the dosage unit. It consists of main ingredients, optional ingredients, and excipients. The functions and proportions of each ingredient are as follows: 1. Main ingredients: Selenium-enriched yeast and dl-α-tocopherol acetate are the core active ingredients of the formula. Selenium-enriched yeast provides plant organic nano-selenium, which participates in immune activation and antioxidant processes. dl-α-tocopherol acetate, as a fat-soluble antioxidant, scavenge fat-soluble free radicals. The two work together to achieve basic immune regulation and antioxidant effects. 2. Optional raw material: L-ascorbic acid (vitamin C), as a water-soluble antioxidant, synergistically with dl-α-tocopherol acetate to construct a lipid-soluble and water-soluble dual antioxidant system, comprehensively scavenging different types of free radicals in the body, while protecting dl-α-tocopherol acetate from oxidation, prolonging its duration of action in the body, and further enhancing immune activation and anti-cancer effects; 3. Excipients: Microcrystalline cellulose is used as a filler to ensure formulation shape and fill volume stability; sodium octenyl succinate starch is used as a dispersant to effectively improve the lipid-soluble aggregation problem of dl-α-tocopherol acetate and enhance its water solubility and in vivo dispersibility; silica is used as an anti-caking agent to prevent powder agglomeration and ensure filling flowability; magnesium stearate is an optional lubricant to further optimize powder filling performance and improve production efficiency.

[0010] The content of the active ingredients in each capsule is strictly controlled to be: 90-100μg selenium and 40-50mg vitamin E, or 90-100μg selenium, 40-50mg vitamin E and 40mg vitamin C. This content meets the recommended daily intake of nutrients for the human body, ensuring efficacy while avoiding the burden on the body caused by excessive intake.

[0011] The raw material ratio for a single-capsule formulation is as follows, by weight: 12-14 parts selenium-enriched yeast, 40-50 parts dl-α-tocopherol acetate, 0-40 parts L-ascorbic acid, 5-8 parts microcrystalline cellulose, 2-4 parts sodium starch octenyl succinate, 1-2 parts silicon dioxide, and 0-0.5 parts magnesium stearate.

[0012] Preferably, the active ingredient content of a single-capsule formulation is: 95 μg selenium and 45 mg vitamin E, or 95 μg selenium, 45 mg vitamin E, and 40 mg vitamin C; the corresponding optimal ratio of raw materials is: 13 parts selenium-enriched yeast, 45 parts dl-α-tocopherol acetate, 40 parts L-ascorbic acid, 6.5 parts microcrystalline cellulose, 3 parts sodium octenyl succinate starch, 1.5 parts silicon dioxide, and 0.3 parts magnesium stearate. Under this ratio, the synergistic effect of each component is the strongest, and the formulation performance and efficacy are both optimal.

[0013] Example 1; An optimized formula of plant-based organic nano-selenium without L-ascorbic acid; Raw material ratio: per single gelatin capsule, 13 parts selenium-enriched yeast, 45 parts dl-α-tocopherol acetate, 6.5 parts microcrystalline cellulose, 3 parts sodium octenyl succinate starch, and 1.5 parts silicon dioxide; Active ingredient content: selenium 95μg, vitamin E 45mg.

[0014] Example 2; The optimized formulation of plant-based organic nano-selenium containing L-ascorbic acid (optimal ratio) contains the following raw material ratios: per single gelatin capsule, 13 parts selenium-enriched yeast, 45 parts dl-α-tocopherol acetate, 40 parts L-ascorbic acid, 6.5 parts microcrystalline cellulose, 3 parts sodium octenyl succinate starch, 1.5 parts silicon dioxide, and 0.3 parts magnesium stearate; the active ingredient content is: selenium 95μg, vitamin E 45mg, and vitamin C 40mg.

[0015] Example 3; A plant-based organic nano-selenium formulation with high content of active ingredients; Raw material ratio: per single gelatin capsule, 14 parts selenium-enriched yeast, 50 parts dl-α-tocopherol acetate, 40 parts L-ascorbic acid, 8 parts microcrystalline cellulose, 4 parts sodium octenyl succinate starch, 2 parts silicon dioxide, and 0.5 parts magnesium stearate; Active ingredient content: 100 μg selenium, 50 mg vitamin E, and 40 mg vitamin C.

[0016] Example 4; A plant-based organic nano-selenium formulation with low content of active ingredients; Raw material ratio: based on a single gelatin empty capsule, 12 parts selenium-enriched yeast, 40 parts dl-α-tocopherol acetate, 5 parts microcrystalline cellulose, 2 parts sodium octenyl succinate starch, and 1 part silicon dioxide; Active ingredient content: selenium 90μg, vitamin E 40mg.

[0017] Based on the above optimized formulation, the present invention also provides a preparation method thereof, comprising the following steps: S1. Raw material pretreatment: Selenium-enriched yeast, dl-α-tocopherol acetate, L-ascorbic acid (if added), microcrystalline cellulose, sodium octenyl succinate starch, silicon dioxide, and magnesium stearate (if added) are passed through a 40-mesh sieve to remove impurities and large particles from the raw materials, ensuring the uniformity of subsequent powder mixing. S2. Powder mixing: Weigh all the raw materials after S1 pretreatment according to the ratio, place them in a three-dimensional mixer, and mix them at a speed of 15~20r / min for 20~30min. Utilize the multi-directional motion characteristics of the three-dimensional mixer to achieve thorough and uniform mixing of the powder and avoid uneven concentration of local components. S3, Capsule Filling: The mixed powder obtained in S2 is added to a fully automatic capsule filling machine and filled using gelatin empty capsules as carriers. The filling volume difference is strictly controlled within ±7.5% to ensure that the content of the active ingredients in each capsule is accurate and consistent. S4. Polishing and Inspection: After filling, the capsules are polished in a capsule polishing machine to remove the floating powder on the surface of the capsules and improve the appearance quality of the preparation. Then, the appearance, fill volume and disintegration time are tested in sequence. The appearance requirements are that the capsules are free from cracks, powder leakage and deformation, the fill volume meets the ratio requirements, and the disintegration time meets the national standard for food-grade capsule preparations. All unqualified products are rejected. S5. Finished Product Packaging: The capsules that have passed the S4 inspection are packaged according to the preset specifications. Food-grade silica gel desiccant is added to each batch of packaging. After sealing the packaging, the finished product is obtained. The desiccant can effectively prevent the preparation from absorbing moisture and clumping, and improve the storage stability of the active ingredients.

[0018] To verify the actual efficacy of the optimized formulation of this invention, the formulations of Examples 1 and 2 were selected and compared with single selenium-enriched yeast and single dl-α-tocopherol acetate in a comparative experiment. The results are as follows: Antioxidant experiment (DPPH free radical scavenging experiment): The DPPH free radical scavenging rate of the formulation of Example 2 reached 92.5%, and that of Example 1 reached 85.3%, which is much higher than that of single selenium-enriched yeast (42.1%) and single dl-α-tocopherol acetate (58.7%), proving that the formulation of this invention has excellent antioxidant effect, and the addition of L-ascorbic acid can further enhance the antioxidant performance; Immunoactivation experiment: The formulation of Example 2 was administered to experimental mice by gavage for 30 consecutive days, and the proliferation capacity of splenic lymphocytes and NK cell activity of mice were detected. The results showed that the proliferation capacity of splenic lymphocytes and NK cell activity of mice were significantly enhanced. Cell proliferation capacity was increased by more than 60%, and NK cell activity was increased by more than 55%, proving that the formulation of this invention can effectively activate the body's immune system; Anti-cancer experiment (in vitro cell culture method): The formulation of Example 2 was applied to HepG2 liver cancer cells, and the results showed that the formulation inhibited the proliferation of HepG2 liver cancer cells by 78.9%, and had no obvious toxicity to normal liver cells, proving that the formulation of this invention has a significant effect on inhibiting the proliferation of cancer cells; Formulation stability experiment: The formulations of Examples 1 and 2 were placed under sealed conditions at room temperature for 12 months, and the retention rate of effective components was tested. The results showed that the retention rate of selenium, vitamin E, and vitamin C (Example 2) was all above 95%, and the capsules showed no moisture absorption, clumping, or breakage, proving that the formulation of this invention has excellent storage stability.

[0019] The above experimental results fully demonstrate that the optimized formulation of plant organic nano-selenium, which activates the immune system, inhibits cancer cell proliferation, prevents cancer, prevents tumors, and has antioxidant and anti-aging effects, has excellent effects in activating the immune system, inhibiting cancer cell proliferation, antioxidation, and anti-aging. The formulation has high stability and excellent bioavailability, and fully achieves the expected effects of the invention.

[0020] The optimized formulation of plant-based organic nano-selenium described in this invention, which activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, can be directly prepared into capsule-type functional foods to meet the needs of different consumer groups. It is suitable for people with low immunity, middle-aged and elderly people seeking anti-aging, people under long-term oxidative stress, and people with cancer and tumor prevention needs. It fills the gap in existing technologies for multi-functional synergistic selenium compound formulations and has extremely high practical value and broad market prospects.

Claims

1. An optimized formulation of plant-based organic nano-selenium that activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, characterized in that... The formulation is based on a single gelatin capsule and consists of a main ingredient, optional ingredients, and excipients. The main ingredient is selenium-enriched yeast and dl-α-tocopherol acetate. The optional ingredient is L-ascorbic acid. The excipients are microcrystalline cellulose, sodium octenyl succinate starch, and silicon dioxide, or microcrystalline cellulose, sodium octenyl succinate starch, silicon dioxide, and magnesium stearate. The content of the active ingredients in a single capsule is: 90-100 μg selenium and 40-50 mg vitamin E, or 90-100 μg selenium, 40-50 mg vitamin E, and 40 mg vitamin C.

2. The optimized formulation of plant-based organic nano-selenium as described in claim 1, which activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, is characterized in that... The raw material ratio for a single-capsule formulation is as follows, by weight: 12-14 parts selenium-enriched yeast, 40-50 parts dl-α-tocopherol acetate, 0-40 parts L-ascorbic acid, 5-8 parts microcrystalline cellulose, 2-4 parts sodium starch octenyl succinate, 1-2 parts silicon dioxide, and 0-0.5 parts magnesium stearate.

3. The optimized formulation of plant-based organic nano-selenium as described in claim 2, which activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, is characterized in that... The preferred active ingredients in a single capsule are: 95 μg selenium and 45 mg vitamin E, or 95 μg selenium, 45 mg vitamin E, and 40 mg vitamin C.

4. The optimized formulation of plant-based organic nano-selenium as described in claim 3, which activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, is characterized in that... The optimal ratio of raw materials for a single-capsule formulation, by weight, is as follows: 13 parts selenium-enriched yeast, 45 parts dl-α-tocopherol acetate, 40 parts L-ascorbic acid, 6.5 parts microcrystalline cellulose, 3 parts sodium octenyl succinate starch, 1.5 parts silicon dioxide, and 0.3 parts magnesium stearate.

5. An optimized formulation of plant-based organic nano-selenium as described in any one of claims 1 to 4, characterized by its preparation method comprising the following steps: S1. Raw material pretreatment: Selenium-enriched yeast, dl-α-tocopherol acetate, L-ascorbic acid (if added), microcrystalline cellulose, sodium octenyl succinate starch, silicon dioxide, and magnesium stearate (if added) are passed through a 40-mesh sieve to remove impurities and are then ready for use. S2. Powder mixing: Weigh all the raw materials after S1 pretreatment according to the ratio, place them in a three-dimensional mixer, and mix at a speed of 15~20r / min for 20~30min to obtain a uniform mixed powder. S3, Capsule Filling: Add the mixed powder obtained in S2 into a fully automatic capsule filling machine, and fill it with gelatin empty capsules as carriers, controlling the filling volume difference within ±7.5%; S4. Polishing and Inspection: Place the filled capsules in a capsule polishing machine to polish them and remove surface powder. Then, conduct appearance, fill volume and disintegration time tests in sequence and reject unqualified products. S5. Finished Product Packaging: The capsules that have passed the S4 inspection are repackaged, food-grade desiccant is added, and then they are sealed and packaged to obtain the finished product.

6. The application of an optimized formulation of plant-based organic nano-selenium as described in any one of claims 1 to 4, which activates the immune system, inhibits cancer cell proliferation, prevents cancer and tumors, and has antioxidant and anti-aging properties, is characterized in that... The formulation is used in the preparation of functional foods that have the effects of activating the immune system, inhibiting cancer cell proliferation, preventing cancer, preventing tumors, anti-oxidation, and anti-aging.