Multi-connected environment DNA (deoxyribonucleic acid) adsorption and preservation bag and use method thereof

Abstract

The invention provides a multi-connected environment DNA adsorption and preservation bag and a use method thereof. The multi-connected environment DNA adsorption and preservation bag comprises a weight falling block, a DNA adsorption layer and a DNA adsorption layer, the thread rope is provided with a long thread head end and a short thread head end, and the weight falling block is arranged on the thread rope and is close to the short thread head end; one end of the height adjusting assembly is movably connected with the thread rope and is located between the weight falling block and the short thread head end; the multi-union filter paper assembly comprises a plurality of rigid macroporous mesh bags and a plurality of pieces of adsorption filter paper with the same number as the rigid macroporous mesh bags, the opening ends of the rigid macroporous mesh bags are all connected with the other end of the height adjusting assembly, and one piece of adsorption filter paper is placed in the corresponding rigid macroporous mesh bag; the weight falling block, the rope, the height adjusting assembly and the multi-union filter paper combination body are all detachably placed in the sealing drying assembly. The multi-connected environment DNA adsorption and preservation bag can be used after being opened and stored after being sealed, can collect multi-connected samples, and is efficient and convenient.

Description

Technical Field

[0001] This invention belongs to the field of sampling device technology, and specifically relates to a multi-linked environmental DNA adsorption and preservation package and its usage method. Background Technology

[0002] Environmental DNA and other particulate matter are widely present in water and air. Sampling and detecting environmental DNA enables non-destructive monitoring of biodiversity, and in recent years it has gradually become a widely used survey and monitoring technology in environmental surveys, ecological protection, and biological research. With the large-scale application of this technology, the use of sampling enrichment devices and consumables has increased significantly.

[0003] Currently, the method for environmental DNA sampling usually involves placing filter media into porous bags to form individual tea bag-like adsorption packs, which are then tied to a weight or shelf and placed in the water for several hours (often requiring a rope to be tied to the shore for easy retrieval). The filter media gradually adsorbs environmental DNA or cell debris from the water. After a period of time, the filter media is removed from the water, taken out of the porous bag, transferred to a preservation tube or bottle, and then preserved with a preservation solution.

[0004] The current technology has several problems: the cumbersome step-by-step operations in the field, such as binding heavy objects, connecting ropes, removing filter media, placing them in preservation tubes, and adding preservation solution, rely on manual on-site operations and lack a unified tool. Furthermore, it cannot be used immediately after opening and sealing, making it very inconvenient to carry and use in the field. Large-scale surveys typically require a large number of samples, but the current individual collection method is generally inefficient and cannot meet the needs of large-scale use. The current process of adding preservation solution generally relies on syringes or pipettes, which is not only inconvenient but also requires carrying a large amount of disposable consumables, creating a burden. Moreover, DNA degradation can easily occur during preservation due to layering and adhesion or failure to dry in time.

[0005] In other words, how to provide a multi-linked environmental DNA adsorption and preservation kit that can be used immediately upon opening and sealing, and can collect multiple samples in a highly efficient and convenient manner is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] The technical problem to be solved by this invention is that current environmental DNA sampling methods are not ready to use immediately, are cumbersome to operate, inefficient, and cannot meet the needs of large-scale use.

[0007] To address the aforementioned problems, a first aspect of the present invention provides a multi-unit environmental DNA adsorption and preservation package, comprising: a weight; a string having a long end and a short end, the weight being disposed on the string and close to the short end; a height adjustment assembly, one end of which is movably connected to the string and located between the weight and the short end; a multi-unit filter paper assembly comprising a plurality of rigid macroporous mesh bags and a plurality of adsorption filter papers, each of the rigid macroporous mesh bags having an open end, the open ends of the plurality of rigid macroporous mesh bags being connected to the other end of the height adjustment assembly, the number of adsorption filter papers being the same as the number of rigid macroporous mesh bags, and one adsorption filter paper being placed inside a corresponding rigid macroporous mesh bag; and a sealing and drying assembly, wherein the weight, the string, the height adjustment assembly, and the multi-unit filter paper assembly are all detachably placed in the sealing and drying assembly.

[0008] In the first aspect, the height adjustment assembly includes: an adjustment sleeve made of an elastic material, the adjustment sleeve being movably sleeved around the periphery of the rope and located between the weight block and the short end of the line, the inner diameter of the adjustment sleeve in its natural state being smaller than the outer diameter of the rope; and a connector, one end of which is provided with a barbed tip made of a rigid material, the barbed tip being detachably inserted into the adjustment sleeve, and the other end of the connector being connected to the opening ends of a plurality of rigid large-hole mesh bags.

[0009] In the first aspect, the connector includes: a plurality of drawstrings, one of the drawstrings being disposed at the opening end of a corresponding rigid large-hole mesh bag; and a thread needle having barbed tips, the thread needle being detachably connected to the plurality of drawstrings.

[0010] In the first aspect, the connector includes: an opening buckle, the opening buckle being made of a material with a density less than water, the opening buckle being detachably connected to the opening ends of a plurality of rigid large-hole mesh bags, the top of the opening buckle being a first annular structure; and a thread-bonded needle, the thread-bonded needle having the barbed tip, the thread-bonded needle being detachably connected to the first annular structure.

[0011] In the first aspect, the connector includes: an opening buckle, the opening buckle being made of a material with a density less than water, the opening buckle being detachably connected to the opening ends of a plurality of rigid large-hole mesh bags; a connecting rope, one end of which is provided with the barbed tip, the other end of which is connected to the opening buckle; and a universal swivel ring, the universal swivel ring having a double bearing structure, the universal swivel ring being disposed on the connecting rope.

[0012] In the first aspect, the opening and closing buckle includes: a buckle body, the top of which is fixedly connected to the other end of the connecting rope, and the bottom of which is provided with an inner engagement groove; a buckle handle, one side of which is rotatably connected to one side of the bottom of the buckle body via an opening and closing buckle shaft, the top of which is provided with engagement teeth, which engage with the inner engagement groove, and the opening ends of several rigid large-hole mesh bags are detachably placed between the buckle body and the buckle handle via the engagement teeth and the inner engagement groove; an inner crank, which is made of elastic material and is located on the other side of the buckle handle; a return hook handle, which is fixed to the top of the inner crank handle; and a trip tooth, which is fixed to the other side of the buckle body and engages with the return hook handle.

[0013] In the first aspect, the sealed drying assembly includes: a self-sealing bag with a sealing strip at its opening, wherein the weight, the rope, the height adjustment assembly, and the multi-sheet filter paper assembly are all placed inside the self-sealing bag; and a desiccant pack placed at the bottom of the self-sealing bag.

[0014] In the first aspect, the sealed drying assembly further includes: a grid frame disposed inside the self-sealing bag, the grid frame having several layers of grid plates, and a corresponding rigid large-pore mesh bag placed between two adjacent layers of grid plates.

[0015] In the first aspect, the weight is made of stainless steel, and the top of the weight is a second ring structure connected to the rope; the rigid large-pore mesh bag has a mesh size greater than 1 mm; the adsorption filter paper is a glass fiber filter membrane with a thickness greater than 400 micrometers and a pore size of 0.7 micrometers.

[0016] The second aspect of this application provides a method for using a multi-unit environmental DNA adsorption and preservation kit. The method includes: opening the sealing strip of a self-sealing bag, removing the multi-unit filter paper assembly, height adjustment component, weight, and string, and then closing the sealing strip; inserting the barbed tip of the height adjustment component into the adjustment sleeve on the string to assemble the multi-unit kit, and adjusting the adjustment sleeve to a preset position; after adjustment, holding the long end of the string, throwing the multi-unit kit into the water; the multi-unit filter paper assembly falling into the water along with the weight; stopping after the weight reaches the bottom; and the multi-unit filter paper... The assembly floats in the water under its own buoyancy, allowing for fixed-point sampling or towing sampling. After sampling, the entire assembly is retrieved along the end of the long line. The barbed tip on the height adjustment component is removed from the adjustment sleeve to remove the multi-sheet filter paper assembly. Residual water droplets are shaken off the assembly, which is then placed into the grid plate on the grid rack in a self-sealing bag. The sealing strip is pinched to seal the bag, and the desiccant packet inside automatically dries and dehydrates the sample on the filter paper. After sealing, the self-sealing bag is sent to the laboratory for subsequent experimental analysis.

[0017] Beneficial Effects: This invention provides a multi-unit environmental DNA adsorption and preservation kit, comprising a weight, a rope, a height adjustment component, a multi-unit filter paper assembly, and a sealing and drying component. The rope has a long end and a short end; the long end can be used to secure it to the shore or a boat, while the short end can be used to connect the height adjustment component and the multi-unit filter paper assembly. The weight is positioned on the rope and near the short end. When the weight is thrown into the water, its gravity causes the height adjustment component, located between the weight and the short end, to sink to the bottom. The other end of the height adjustment component is connected to the open ends of several rigid macroporous mesh bags in the multi-unit filter paper assembly, allowing these bags to sink to the bottom along with the height adjustment component. Environmental DNA is adsorbed in the water by the adsorption filter paper placed inside each rigid macroporous mesh bag. Multiple samples can be collected simultaneously using several adsorption filter papers, the same number as the number of rigid macroporous mesh bags. Highly efficient and convenient, the rigid, large-pore mesh bag material possesses a certain degree of rigidity. When used in triplicate or multi-unit configurations, the slightly rigid mesh bag provides sufficient space for the internal adsorption filter paper, preventing cross-contamination between samples. One end of the height adjustment component is movably connected to the string, allowing adjustment of the distance between the multi-unit filter paper assembly and the bottom of the water body. This flexibly addresses the sampling needs of different water layers. The weight, string, height adjustment component, and multi-unit filter paper assembly are all detachably placed in the sealed drying assembly. When used in the field, these components can be directly removed from the sealed drying assembly and placed into the water body for environmental DNA sampling. After sampling, the multi-unit filter paper assembly can be directly returned to the sealed drying assembly for sealing and drying preservation. This allows for immediate use and storage, eliminating the tedious step-by-step operations in the field, making it highly efficient and convenient. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this specification or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of the multi-linked environmental DNA adsorption and preservation package provided in the embodiments of the present invention;

[0020] Figure 2 This is a schematic diagram of the structure of the multi-linked environmental DNA adsorption and preservation package provided in the embodiments of the present invention during its use. Figure 3 This is a schematic diagram of a height adjustment component provided in an embodiment of the present invention; Figure 4 This is a side view of the grid frame provided in an embodiment of the present invention.

[0021] Explanation of reference numerals in the attached figures: 1. Weighted block; 11. Second ring structure; 2. Thread; 21. Long thread end; 22. Short thread end; 3. Height adjustment assembly; 31. Adjustment sleeve; 32. Connector; 321. Opening and closing buckle; 3211. Buckle body; 3212. Inner engagement groove; 3213. Buckle handle; 3214. Opening and closing buckle shaft; 3215. Engaging teeth; 3216. Inner crank; 3217. Return hook handle; 3218. Trip teeth; 3219. First ring structure; 322. Connecting rope; 323. Universal swivel; 33. Barbed tip; 4. Multi-unit filter paper assembly; 41. Rigid large-pore mesh bag; 42. Adsorption filter paper; 5. Sealed drying assembly; 51. Self-sealing bag; 52. Sealing strip; 53. Desiccant pack; 54. Grille rack. Detailed Implementation

[0022] The technical solutions of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention are within the scope of protection of the present invention.

[0023] Furthermore, throughout this specification, unless otherwise specified, the terminology used herein should be understood as having the meaning commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the event of any conflict, this specification shall prevail.

[0024] Example 1: like Figure 1-4As shown, this embodiment provides a multi-link environmental DNA adsorption and preservation kit, including a weight block 1, a string 2, a height adjustment component 3, a multi-link filter paper assembly 4, and a sealing and drying component 5, wherein: the string 2 has a long end 21 and a short end 22, the weight block 1 is disposed on the string 2 and close to the short end 22; one end of the height adjustment component 3 is movably connected to the string 2 and is located between the weight block 1 and the short end 22; the multi-link filter paper assembly 4 includes a plurality of rigid large pores. The system comprises a mesh bag 41 and several adsorption filter papers 42. Each rigid, large-pore mesh bag 41 has an open end, and the open ends of all the rigid, large-pore mesh bags 41 are connected to the other end of the height adjustment component 3. The number of adsorption filter papers 42 is the same as the number of rigid, large-pore mesh bags 41, with one adsorption filter paper 42 placed inside a corresponding rigid, large-pore mesh bag 41. The weight block 1, the rope 2, the height adjustment component 3, and the multi-link filter paper assembly 4 are all detachably placed in the sealing and drying component 5. The rope 2 is a fishing line.

[0025] Specifically, the present invention provides a multi-link environmental DNA adsorption and preservation kit, comprising a weight 1, a rope 2, a height adjustment component 3, a multi-link filter paper assembly 4, and a sealing and drying component 5. The rope 2 has a long end 21 and a short end 22. The long end 21 can be used to be moored on the shore or on a boat, and the short end 22 can be used to connect the height adjustment component 3 and the multi-link filter paper assembly 4. The weight 1 is positioned on the rope 2 and close to the short end of the rope 2. When the weight 1 is thrown into the water, DNA can be adsorbed and preserved through the water. Gravity causes the height adjustment component 3, located between the weight block 1 and the short line end 22, to sink to the bottom of the water. The other end of the height adjustment component 3 is connected to the open ends of several rigid macroporous mesh bags 41 of the multi-link filter paper assembly 4, causing these bags to sink to the bottom along with the height adjustment component 3. Environmental DNA is adsorbed in the water through the adsorption filter paper placed inside each rigid macroporous mesh bag 41. The adsorption filter paper 42, in a number equal to the number of rigid macroporous mesh bags 41, allows for the adsorption of environmental DNA in the water. Simultaneous collection of multiple samples is efficient and convenient. The rigid large-pore mesh bag 41 has a certain degree of rigidity. When using three or more samples, the slightly rigid mesh bag can provide some space for the adsorption filter paper 42 inside, preventing cross-contamination between multiple samples. One end of the height adjustment component 3 is movably connected to the rope 2. By adjusting the position of one end of the height adjustment component 3 on the rope, the distance between the multiple filter paper assembly 4 and the bottom of the water body can be adjusted, which can flexibly meet the needs of sampling different water layers. The weight block 1, rope 2, height adjustment component 3, and multiple filter paper assembly 4 can all be detachably placed in the sealed drying component 5. When used in the field, the weight block 1, rope 2, height adjustment component 3, and multiple filter paper assembly 4 can be directly removed from the sealed drying component 5 and placed into the water body for environmental DNA sampling. After sampling, the multiple filter paper assembly 4 can be directly placed back into the sealed drying component 5 for sealing and drying preservation. It can be used immediately after opening and sealed immediately after storage, saving the tedious step-by-step operation in the field, which is efficient and convenient.

[0026] In some possible implementations, the height adjustment assembly 3 includes: an adjustment sleeve 31 made of an elastic material, specifically, the adjustment sleeve 31 may be made of rubber material, the adjustment sleeve 31 is movably sleeved around the rope 2 and located between the weight block 1 and the short line end 22, the inner diameter of the adjustment sleeve 31 in its natural state is smaller than the outer diameter of the rope 2; a connector 32, one end of the connector 32 is provided with a barbed tip 33 made of a rigid material, the barbed tip 33 is detachably inserted into the adjustment sleeve 31, and the other end of the connector 32 is connected to the opening ends of a plurality of rigid large-hole mesh bags 41.

[0027] This is because the adjusting sleeve 31, made of elastic material, is movably fitted around the outer periphery of the rope 2 and located between the weight block 1 and the short end 22 of the rope. This allows the position of the adjusting sleeve 31 on the short end 22 of the rope 2 to be adjustable, thereby adjusting the distance between the multi-link filter paper assembly 4 and the weight block 1. This, in turn, adjusts the distance between the multi-link adsorption filter paper 42 and the rigid large-pore mesh bag 41 and the bottom of the water when deployed. The distance can be adjusted to the optimal level according to different water depths, facilitating better sampling and flexibly meeting the needs of sampling at different water layers. Furthermore, the inner diameter of the adjusting sleeve 31 in its natural state is smaller than the outer diameter of the rope 2, allowing the adjusting sleeve 31 to be positioned between the rope 2 and the rope 2 with a more flexible and controlled distance. The friction generated by the interference fit is relatively large. The elastic compression force of the adjusting sleeve 31 can be used to fix the adjusting sleeve 31 to the rope 2. The position can be adjusted by simply moving the adjusting sleeve 31 along the rope 2. Once adjusted, it is not easy to move, making the operation convenient. One end of the connector 32 is provided with a barbed tip 33 that can be detachably inserted into the adjusting sleeve 31. The other end of the connector 32 is connected to the opening end of several rigid large-hole mesh bags 41. The rigidity and unique barbed structure of the barbed tip 33 make it easy to insert into the elastic adjusting sleeve 31 for fixation. The barbed tip 33 and the connector 32 connect several rigid large-hole mesh bags 41 and the adsorption filter paper 42 inside the rigid large-hole mesh bags 41 to the adjusting sleeve 31.

[0028] In some possible implementations, the connector 32 includes: a plurality of drawstrings, one of which is disposed at the opening end of a corresponding rigid large-hole mesh bag 41; and a thread-bonded needle having barbed tips 33, wherein the thread-bonded needle is detachably connected to the plurality of drawstrings. Specifically, one end of the thread-bonded needle is a barbed tip, and the other end is a horizontal hole end, the barbed tip end and the horizontal hole end being adapted to each other, and the thread-bonded needle can self-close to form a shrink ring.

[0029] Those skilled in the art will understand that a drawstring is provided at the opening end of a corresponding rigid large-pore mesh bag 41. By pulling the drawstring towards the distal end, the opening end of the rigid large-pore mesh bag 41 can be tightened to prevent the adsorption filter paper 42 inside the rigid large-pore mesh bag 41 from falling out. The adhesive needle has a barbed tip 33. The adhesive needle is detachably connected to several drawstrings. The adhesive needle can pass through several drawstrings and then self-enclose to form a shrink ring, or the adhesive needle can first self-enclose to form a shrink ring and then tie several drawstrings to the shrink ring to connect several drawstrings to the adhesive needle. The barbed tip 33 that protrudes after the adhesive needle is enclosed can then be further inserted into the adjusting sleeve 31 to fix several drawstrings to the adjusting sleeve 31 through the adhesive needle.

[0030] In some possible implementations, the connector 32 includes: an opening buckle 321, the opening buckle 321 being made of a material with a density less than water, the opening buckle 321 being detachably connected to the opening ends of a plurality of rigid large-hole mesh bags 41, the top of the opening buckle 321 being a first annular structure 3219; and a thread needle having the barbed tip 33, the thread needle being detachably connected to the first annular structure 3219.

[0031] Those skilled in the art will understand that the detachable connection between the opening buckle 321 and the opening ends of several rigid large-pore mesh bags 41 allows the opening ends of several rigid large-pore mesh bags 41 to be placed inside by opening the opening buckle 321, and then the opening buckle 321 to close the opening ends of the rigid large-pore mesh bags 41, preventing the adsorbent filter paper 42 from falling out, and also allowing the several rigid large-pore mesh bags 41 to be connected and fixed; the adhesive needle has a barbed tip 33, and the adhesive needle is detachably connected to the first annular structure 3219 at the top of the opening buckle 321, allowing the adhesive needle to pass through the first annular structure 3219 and then self-enclose to form a shrinking ring, and the barbed tip 33 that has passed through after enclosing further passes into the adjusting sleeve 31, so as to connect the opening buckle 321 and the multi-link filter paper assembly 3 on the opening buckle 321 to the adjusting sleeve 31.

[0032] In some possible embodiments, the connector 32 includes: an opening and closing buckle 321, the opening buckle 321 being made of a material with a density less than water, specifically, the opening and closing buckle 321 being made of polypropylene, and the opening buckle 321 being detachably connected to the opening ends of a plurality of rigid large-pore mesh bags 41; a connecting rope 322, one end of which is provided with the barbed tip 33, and the other end of which is connected to the opening buckle 321; and a universal swivel ring 323, the universal swivel ring 323 being a double-bearing structure, specifically, the universal swivel ring being a 360-degree double-bearing universal swivel ring, and the universal swivel ring 323 being disposed on the connecting rope 322. The density of both the rigid large-pore mesh bag 41 and the density of the adsorption filter paper 42 are less than that of water.

[0033] This is because the detachable connection between the opening buckle 321 and the opening ends of several rigid large-pore mesh bags 41 allows for quick and convenient locking of the opening ends of one or more rigid large-pore mesh bags 41 via the "guillotine" design of the opening buckle 321, facilitating the subsequent removal of the absorbent filter paper 42 containing the sample. One end of the connecting rope 322 is equipped with a barbed tip 33, and the other end is connected to the opening buckle 321, so that the opening buckle 321 and the multi-link filter paper assembly 3 on the opening buckle 321 are connected to the adjusting sleeve 31 via the connecting rope 322. The universal swivel ring 323 set on the connecting rope 322 has a double bearing structure. The friction of the universal swivel ring 323 is extremely small. Under the weak external force of water flow impact or tugboat towing, it can achieve 360-degree self-rotation, which greatly increases the contact frequency and contact area between the absorbent filter paper 42 and the water, thereby increasing D. The adsorption and enrichment of particles such as Na increases the DNA capture concentration per unit time. The rotating shaft is designed as a dual-shaft structure, with one shaft locked while the other can still rotate flexibly. The opening and closing buckle 321 is made of a material with a density slightly less than water. The rigid macroporous mesh bag 41 and the adsorption filter paper 42 also have a relatively low density, allowing the three components to float and unfold in the water. This avoids sinking to the bottom and contacting the sediment, which would affect the effectiveness of collecting DNA from the water. It also allows for a larger contact area with the water, which is beneficial for adsorbing the DNA sample. Furthermore, the overall floating (suspended) design combined with the spin design of the universal rotating ring 323 allows it to rotate freely in the water, greatly increasing the contact between the adsorption filter paper 42 and the water. This results in a higher concentration of DNA being captured within a certain time, achieving higher efficiency in DNA enrichment. At the same time, it also prevents self-entanglement or sinking into the sediment, thereby reducing experimental errors. In addition, through the overall floating (suspension) and the spin design of the universal rotating ring 323, when moving and dragging for sampling, the multi-link filter paper assembly 4 will rotate at high speed due to the continuous relative force generated by the water flow on the rigid large-pore mesh bag 41. The eddy current effect generated by this rotation significantly increases the contact flux between the adsorption filter paper 42 and the water body, and can enrich higher concentrations of DNA in a shorter time.

[0034] In some possible implementations, the opening and closing buckle 321 includes: a buckle body 3211, the top of which is fixedly connected to the other end of the connecting rope 322, and the bottom of the buckle body 3211 is provided with an inner engagement groove 3212; a buckle handle 3213, one side of which is rotatably connected to one side of the bottom of the buckle body 3211 via an opening and closing buckle shaft 3214, the top of which is provided with engagement teeth 3215, and the engagement teeth 3215 are engaged with the inner engagement groove 3212; and a plurality of rigid large-hole mesh bags 41. The open end is detachably placed between the buckle body 3211 and the buckle handle 3213 via the engagement tooth 3215 and the inner engagement groove 3212; the inner crank 3216 is made of elastic material and is located on the other side of the buckle handle 3213; the return hook handle 3217 is fixed to the top of the inner crank 3216; the trip tooth 3218 is fixed to the other side of the buckle body 3211 and is fastened to the return hook handle 3217.

[0035] This is because the top of the buckle body 3211 is fixedly connected to the other end of the connecting rope 322, and one side of the buckle handle 3213 is rotatably connected to one side of the bottom of the buckle body 3211 through the opening and closing buckle shaft 3214, thus forming a "guillotine" design between the buckle body 3211 and the buckle handle 3213; the inner engagement groove 3212 set at the bottom of the buckle body 3211 is engaged with the engagement teeth 3215 set at the top of the buckle handle 3213, and the opening ends of several rigid large-hole mesh bags 41 are detachably placed between the buckle body 3211 and the buckle handle 3213 through the engagement teeth 3215 and the inner engagement groove 3212; the inner crank 3216 made of elastic material is set on the other side of the buckle handle 3213, the hook handle 3217 is fixed to the top of the inner crank 3216, and the trip tooth 3218 fixed on the other side of the buckle body 3211 is engaged with the hook handle 3217. When the "guillotine" design of the buckle 3213 is closed, the interlocking teeth 3215 on its inner side engage with the inner interlocking groove 3212 of the buckle body 3211, pressing and closing the opening end of the rigid large-pore mesh bag 41. At the same time, under the elastic action of the inner crank 3216 on the buckle 3213, the hook handle 3217 engages with the trip teeth 3218, locking the entire bag in a secure and stable manner, preventing the opening end of the rigid large-pore mesh bag 41 from slipping off. Through the design of the inner crank 3216 and the hook handle 3217, a self-locking mechanism is formed when closed, ensuring a very secure fit. When opening, simply press the end of the inner crank 3216 to open it directly without damaging the mesh bag. This allows users to easily and quickly open and close the buckle 321 by simply pressing the hook handle 3217, facilitating the removal of the filter membrane for subsequent experiments.

[0036] In some possible implementations, the sealed drying assembly 5 includes: a self-sealing bag 51 with a sealing strip 52 at its opening, the weight block 1, the rope 2, the height adjustment assembly 3, and the multi-sheet filter paper assembly 4 all placed inside the self-sealing bag 51; and a desiccant pack 53 placed at the bottom of the self-sealing bag 51. The desiccant pack 53 comprises a breathable non-woven fabric bag and a desiccant. The desiccant fills the interior of the non-woven fabric bag. The desiccant is preferably silica gel granules, which can be used on a large scale and is relatively safe to store. The desiccant weighs 40 grams; the use of an excess of high-quality desiccant allows the DNA sample to be dehydrated in a short time.

[0037] This is because the opening of the self-sealing bag 51 is equipped with a sealing strip 52. By opening the sealing strip 52, the weight block 1, the string 2, the height adjustment component 3, and the multi-sheet filter paper assembly 4 can be taken out or put into the self-sealing bag 51. Closing the sealing strip 52 achieves the sealing of the self-sealing bag 51, enabling immediate use. The bottom of the self-sealing bag 51 is equipped with a desiccant pack 53, allowing the self-sealing bag 51 to be used for sample preservation. After collecting the DNA sample, the multi-sheet filter paper assembly can be removed, the water droplets shaken off, and the sample placed directly into the self-sealing bag 51. Then, the sealing strip is tightened, achieving immediate sealing and storage. At the same time, the desiccant pack 53 allows the DNA sample to be dehydrated in a short time, inhibiting microbial activity and thus maintaining the stability of DNA and other biomolecules for a long time, greatly reducing degradation. DNA can be stored at room temperature for a long time without freezing, thereby reducing experimental errors and facilitating carrying in field operations.

[0038] In some possible implementations, the sealed drying assembly 5 further includes a grid frame 54 disposed inside the self-sealing bag 51. The grid frame 54 has several layers of grid plates, and a corresponding rigid large-pore mesh bag 41 is placed between two adjacent layers of grid plates. The grid frame is located in the upper middle part of the self-sealing bag 51.

[0039] This is because the grid frame 54 inside the self-sealing bag 51 has several layers of grid plates. A corresponding rigid large-pore mesh bag 41 and the adsorption filter paper 42 inside can be placed in the gap between two adjacent grid plates, thereby isolating each adsorption filter paper 42 and protecting it, avoiding cross-contamination between multiple samples. At the same time, the space provided by the grid frame 54 also facilitates the dehydration of the desiccant, allowing it to dry faster and thus extending the DNA preservation time. In addition, during transportation and storage, shaking will cause the weight block 1 to move inside the self-sealing bag 51. The grid frame 54 can also prevent the weight block 1 from pressing on the adsorption filter paper 42 and breaking it.

[0040] In some possible embodiments, the weight block 1 is made of stainless steel, and the top of the weight block 1 is a second ring structure 11, which is connected to the rope 2; the rigid macroporous mesh bag 41 has a mesh size greater than 1 mm, and the rigid macroporous mesh bag 41 is made of a rigid material with a density less than water. Specifically, the rigid macroporous mesh bag 41 can be made of high-density polyethylene; the adsorption filter paper 42 is a glass fiber filter membrane, and the thickness of the adsorption filter paper 42 is greater than 400 micrometers and the pore size is 0.7 micrometers.

[0041] Those skilled in the art will understand that the top of the weight block 1, made of stainless steel, is a second ring structure 11. The weight block 1 can be tied to one end of the rope 2 through the second ring structure 11, thus forming a short line end 22 and a long line end 21, which facilitates connection. The mesh diameter of the rigid macroporous mesh bag 41 is greater than 1 mm to reduce the influence of surface tension, allowing the water sample to exchange more freely inside and outside the mesh, which is conducive to the adsorption filter paper fully contacting the water sample and adsorbing the DNA in the water sample. The rigid macroporous mesh bag 41 is made of a rigid material with a density less than water, which can provide a certain space for the adsorption filter paper 42, so that when it is stuffed into the gap of the grid frame 54 of the self-sealing bag 51, the adsorption filter paper 42 will not contact the grid frame 54, avoiding contamination. Moreover, during storage, the rigid macroporous mesh bag 41 and the grid frame 54 can separate the adsorption filter paper 42 to prevent adhesion and cross-contamination.

[0042] In the initial state, the multi-link environmental DNA adsorption and preservation package of the present invention has the weight block 1, the string 2, the height adjustment component 3, and the multi-link filter paper assembly 4 all located inside the self-sealing bag 51, and the weight block 1 and the string 2 are in an assembled state. The adjustment sleeve 31 of the height adjustment component 3 is located on the string 2, and the connector 32, the barbed tip 33, and the multi-link filter paper assembly 4 of the height adjustment component 3 are in an assembled state. When used in the field, simply open the sealing strip 52 of the self-sealing bag 51, remove the weight block 1, rope 2, height adjustment component 3, and multi-filter paper assembly 4 from the grid frame 54, insert the barbed tip 33 into the adjustment sleeve 31 to form the complete multi-filter paper assembly, hold the long end 21, and throw it into the target water area. After the multi-filter paper 42 and rigid large-pore mesh bag 41 sink to the bottom with the weight block 1, tie the long end 21 to the shore to perform static sampling. After waiting for a certain period of time (usually several hours), the multi-filter paper 42 will have absorbed water. With sufficient environmental DNA attached, the multi-linked adsorption filter paper 42 and the rigid macroporous mesh bag 41, along with their connected ropes 2 and weights 1, are retrieved from the long line 21 moored on the shore. After removing the rigid macroporous mesh bag 41 and shaking off the water droplets, each bag is placed directly into the gaps of the grid frame 54 in the original self-sealing bag 51. The sealing strip is then pinched tight, allowing the desiccant packet in the self-sealing bag 51 to gradually dry and dehydrate the sample, thus achieving long-term stable preservation of environmental DNA. In this way, the field environmental DNA adsorption sampling tool can be used immediately and sealed for immediate storage.

[0043] In addition to static sampling, the multi-link environmental DNA adsorption and preservation kit of this application is particularly suitable for mobile sampling. The long cable end 21 is fixed to the bottom of an unmanned or ordinary boat, and sampling is carried out while the boat is sailing. During sampling, the water flow generates a continuous relative force on the rigid macroporous mesh bag 41. With the help of the 360-degree double-bearing universal swivel ring on the connecting rope, the multi-link filter paper assembly 4 will rotate at high speed. The eddy current effect generated by this rotation significantly increases the contact flux between the adsorption filter paper 42 and the water body. Compared with static sampling, it can enrich higher concentrations of DNA in a shorter time.

[0044] Example 2: Embodiment 2 of this application provides a method for using a multi-unit environmental DNA adsorption and preservation kit. The method includes: opening the sealing strip 52 of the self-sealing bag 51, taking out the multi-unit filter paper assembly 4, the height adjustment component 3, the weight block 1, and the string 2, and then closing the sealing strip 52; inserting the barbed tip 33 on the height adjustment component 3 into the adjusting sleeve 31 on the string 2 to form a complete multi-unit kit, and adjusting the adjusting sleeve 31 to a preset position; after adjustment, holding the long end 21 of the string 2, throwing the complete multi-unit kit into the water, the multi-unit filter paper assembly 4 falls into the water along with the weight block 1, and stops after the weight block 1 reaches the bottom of the water, and the multi-unit filter paper assembly... The assembly 4 floats in the water under its own buoyancy, allowing for fixed-point sampling or towing sampling. After sampling, the entire assembly is retrieved along the end 21 of the long line. The barbed tip 33 on the height adjustment component 3 is removed from the adjustment sleeve 31 to remove the multi-link filter paper assembly 4. Residual water droplets on the multi-link filter paper assembly 4 are shaken off, and it is placed into the grid plate on the grid frame 54 in the self-sealing bag 41. The sealing strip 52 is pinched to seal the bag, and the desiccant packet 53 inside the self-sealing bag 51 automatically dries and dehydrates the sample on the adsorption filter paper 42. After sealing, the self-sealing bag 51 is sent to the laboratory for subsequent experimental analysis. Specifically, during experimental analysis, the multi-link filter paper assembly 4 can be removed from the self-sealing bag 51, and the adsorption filter paper 42 can be removed from the rigid large-pore mesh bag 41 for analysis.

[0045] In some possible implementations, the fixed-point sampling includes tying the long line end 21 to the shore for sampling; the mobile towing sampling includes fixing the long line end 21 to the bottom of an unmanned boat or a regular boat and sampling during the boat's voyage.

[0046] It should be noted that the method of using the multi-linked environmental DNA adsorption and preservation package provided in this embodiment 2 uses the same multi-linked environmental DNA adsorption and preservation package as described in this embodiment 1. Its implementation principle and technical concept are exactly the same as those in embodiment 1. Therefore, for the parts not described in detail in this embodiment 2, please refer to embodiment 1. They will not be repeated here.

[0047] Finally, it should be noted that the above embodiments are merely specific implementations of the present invention, used to illustrate the technical solutions of the present invention, and not to limit it. The scope of protection of the present invention is not limited thereto. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments within the scope of the technology disclosed in the present invention, or make equivalent substitutions for some of the technical features; and these modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention. All should be covered within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

[0048] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and illustrations shown and described herein.

Claims

1. A multi-linked environmental DNA adsorption and preservation package, characterized in that, include: Heavy object falling; A rope having a long end and a short end, wherein the weight is disposed on the rope and near the short end; A height adjustment component, one end of which is movably connected to the rope and located between the weight block and the short line end; A multi-link filter paper assembly includes several rigid macroporous mesh bags and several adsorption filter papers. Each rigid macroporous mesh bag has an open end, and the open ends of several rigid macroporous mesh bags are connected to the other end of the height adjustment component. The number of adsorption filter papers is the same as the number of rigid macroporous mesh bags, and one adsorption filter paper is placed inside a corresponding rigid macroporous mesh bag. The sealed drying assembly includes a weight, a rope, a height adjustment assembly, and a multi-filter paper assembly, all of which can be detachably placed within the sealed drying assembly.

2. The multi-connected environmental DNA adsorption and preservation package according to claim 1, characterized in that, The height adjustment component includes: An adjusting sleeve, which is made of elastic material, is movably sleeved around the rope and located between the weight block and the short end of the rope. The inner diameter of the adjusting sleeve in its natural state is smaller than the outer diameter of the rope. A connector, one end of which is provided with a barbed tip made of rigid material, the barbed tip being detachably inserted into the adjusting sleeve, and the other end of which is connected to the opening ends of several rigid large-hole mesh bags.

3. The multi-linked environmental DNA adsorption and preservation package according to claim 2, characterized in that, The connector includes: Several drawstrings are provided, one of which is provided at the opening end of a corresponding rigid large-hole mesh bag. The thread-bonded needle has the barbed tip and is detachably connected to a plurality of the drawstring cords.

4. The multi-connected environmental DNA adsorption and preservation package according to claim 2, characterized in that, The connector includes: The opening buckle is made of a material with a density less than water. The opening buckle is detachably connected to the opening ends of several rigid large-hole mesh bags. The top of the opening buckle is a first ring structure. The thread-bonded needle has the barbed tip and is detachably connected to the first annular structure.

5. The multi-linked environmental DNA adsorption and preservation package according to claim 2, characterized in that, The connector includes: The opening buckle is made of a material with a density less than water, and the opening buckle is detachably connected to the opening ends of several rigid large-hole mesh bags. A connecting rope, one end of which is provided with the barbed tip, and the other end of which is connected to the open buckle; The universal swivel has a double bearing structure and is mounted on the connecting rope.

6. The multi-connected environmental DNA adsorption and preservation package according to claim 5, characterized in that, The opening and closing latch includes: The buckle body has its top fixedly connected to the other end of the connecting rope, and its bottom is provided with an inner engagement groove. The buckle handle is rotatably connected to one side of the bottom of the buckle body via an opening and closing buckle shaft. The top of the buckle handle is provided with a biting tooth, which is engaged with the inner biting groove. The opening ends of several rigid large-hole mesh bags are detachably placed between the buckle body and the buckle handle through the biting tooth and the inner biting groove. An inner crank, which is made of an elastic material, is located on the other side of the buckle handle; A return hook lever, which is fixed to the top of the inner crank; The trip tooth is fixed to the other side of the buckle body and is fastened to the return hook handle.

7. The multi-connected environmental DNA adsorption and preservation package according to claim 1, characterized in that, The sealed drying assembly includes: The self-sealing bag has a sealing strip at its opening, and the weight, the rope, the height adjustment component, and the multi-sheet filter paper assembly are all placed inside the self-sealing bag. A desiccant packet is placed at the bottom of the self-sealing bag.

8. The multi-linked environmental DNA adsorption and preservation package according to claim 7, characterized in that, The sealed drying assembly also includes: A grid frame is disposed inside the self-sealing bag. The grid frame has several layers of grid plates, and a corresponding rigid large-hole mesh bag is placed between two adjacent layers of grid plates.

9. The multi-linked environmental DNA adsorption and preservation package according to claim 8, characterized in that: The weight block is made of stainless steel, and the top of the weight block has a second ring structure, which is connected to the rope. The rigid large-pore mesh bag has a mesh diameter greater than 1 mm and is made of a rigid material with a density less than that of water. The adsorption filter paper is a glass fiber filter membrane with a thickness greater than 400 micrometers and a pore size of 0.7 micrometers.

10. A method of using the multi-linked environmental DNA adsorption and preservation package as described in any one of claims 1-9, characterized in that, The method of use includes: Open the sealing strip of the self-sealing bag, take out the multi-filter paper assembly, height adjustment component, weight block and rope, and then close the sealing strip. Insert the barbed tip on the height adjustment component into the adjustment sleeve on the rope to form the complete multi-filter paper assembly, and adjust the adjustment sleeve to the preset position. After adjustment, hold the long end of the string and throw the entire multi-unit filter paper assembly into the water. The multi-unit filter paper assembly falls into the water along with the weight block. After the weight block reaches the bottom of the water and stops, the multi-unit filter paper assembly floats in the water under its own buoyancy, allowing for fixed-point placement sampling or moving and dragging sampling. After sampling, retrieve the complete multi-piece filter paper assembly by following the end of the long line. Remove the barbed tip on the height adjustment component from the adjustment sleeve to remove the multi-piece filter paper assembly. Shake off any residual water droplets on the multi-piece filter paper assembly and place it into the grid plate on the grid rack in the self-sealing bag. Pinch the sealing strip to seal the bag. The desiccant pack inside the self-sealing bag will automatically dry and dehydrate the sample on the filter paper. After sealing, the resealable bag will be sent to the laboratory for further experimental analysis.

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