Wide-field fluorescence lifetime imaging system based on streak camera and imaging method thereof

By introducing a fluorescence lifetime imaging system that combines a reference camera and a streak camera working in parallel, the problems of high photon loss and low imaging efficiency in existing technologies have been solved, achieving high-precision fluorescence lifetime imaging and improving the system's stability and imaging efficiency.

CN122084592AActive Publication Date: 2026-05-26SHENZHEN UNIV
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Patent Information

Application Number
CN202610552294.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-24
Publication Date
2026-05-26
Estimated Expiration
2046-04-24

AI Technical Summary

Technical Problem

Existing fluorescence lifetime imaging systems based on streak cameras suffer from high photon loss and low imaging efficiency, especially when using DMD encoders, where photon efficiency is reduced and system stability and robustness are limited.

Method used

The reference camera and the streak camera work in parallel. The fluorescence signal is split into two paths by a beam splitter. The reference camera acquires a real-time two-dimensional spatial intensity image as the physical spatial reference of the system. Combined with the synchronization matching module, the excitation source and the streak camera are synchronized, avoiding DMD coding and complex reconstruction algorithms, and ensuring photon utilization and imaging accuracy.

Benefits of technology

It achieves high-precision fluorescence lifetime reconstruction, reduces hardware costs and data processing pressure, improves imaging real-time performance and stability, supports real-time monitoring at higher frame rates, and enhances the detection accuracy of weak fluorescence samples.

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Abstract

The invention relates to the field of fluorescence lifetime imaging, and discloses a wide-field fluorescence lifetime imaging system based on a streak camera and an imaging method thereof.The wide-field fluorescence lifetime imaging system comprises an excitation light source, a wide-field excitation module, a synchronous detection module and a synchronous matching module, and the wide-field excitation module is arranged on a transmission path of pulse laser; the synchronous detection module comprises a collection light splitting assembly, a reference camera and a streak camera assembly, the light inlet end of the collection light splitting assembly is used for receiving a fluorescent light beam emitted by a sample, the light outlet end of the collection light splitting assembly divides the fluorescent light beam into a first detection light path and a second detection light path, and the reference camera is arranged on the first detection light path; the streak camera assembly is arranged on the second detection light path; the synchronous matching module is electrically connected with the excitation light source, the reference camera and the streak camera assembly. According to the invention, the problem of fluorescent photon loss caused by a complex optical coding scheme of a traditional DMD is avoided, a complex reconstruction algorithm is not needed, and the imaging efficiency is improved.
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