Preparation method and application of car-t cells loaded with biocalcified nanomaterials
By preparing BCLT, a biocalcified nanomaterial with mitochondrial targeting on its surface, and linking it to CAR-T cells, the problems of poor infiltration effect and immunosuppressive microenvironment of CAR-T cells in solid tumor treatment were solved, achieving highly efficient tumor killing and infiltration effects, and improving treatment efficacy and mouse survival rate.
CN122097569APending Publication Date: 2026-05-29XUZHOU MEDICAL UNIVERSITY
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- XUZHOU MEDICAL UNIVERSITY
- Filing Date
- 2026-03-04
- Publication Date
- 2026-05-29
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Figure CN122097569A_ABST
Abstract
The application is suitable for the field of biological medicine technology, and provides a preparation method and application of CAR-T cells loaded with biological calcified nanomaterials, which comprises the following steps: step one, mixing black phosphorus quantum dots, calcium peroxide and lipid components, and purifying by ultrasonic emulsification and ultrafiltration centrifugation to obtain BCLT with mitochondria-targeting molecule triphenylphosphine modified on the surface; step two, co-incubating CAR-T cells with membrane dye after staining, and adding a disulfide bond-containing reduction-responsive linker arm DSPE-S-S-NHS; and step three, co-incubating BCLT with CAR-T cells, so that the BCLT is connected to the surface of the CAR-T cells through a chemical bond to obtain CAR-T cells loaded with biological calcified nanomaterials. The application significantly improves the infiltration capacity and killing effect of the CAR-T cells on solid tumors, can relieve the problem of tumor heterogeneity, and has excellent biological safety and synergistic anti-tumor effect.
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