Organic fertilizer, method for preparing the same and use thereof

Abstract

The application relates to the field of fertilizers, and discloses an organic fertilizer and a preparation method and application thereof. The content of Ca in the organic fertilizer is 0.9-2.5 wt%, the content of Cl is 3.1-6.9 wt%, the content of Mg is 0.05-0.15 wt%, and the content of small-molecule animal protein is 0.25-0.75 wt% on a dry basis. The preparation method comprises the following steps: concentrating bone gelatin production waste liquid to obtain a concentrated solution, and mixing the concentrated solution and organic fertilizer preparation raw materials; the organic fertilizer preparation raw materials are livestock manure and / or animal and plant residues. The organic fertilizer has good fertilizer efficiency, can improve crop yield and improve the quality of agricultural products in agricultural production, and can also improve the soil. The preparation method can improve the specific gravity of the organic fertilizer powder and granules, especially can improve the balling rate, glossiness of the granules, shorten the disintegration time, and further shorten the production cycle of the organic fertilizer, improve the fertilizer efficiency of the organic fertilizer, and improve the sales radius.

Description

Technical Field

[0001] This invention relates to the field of fertilizers, specifically to an organic fertilizer, its preparation method, and its application. Background Technology

[0002] The production of gelatin from animal bones generates a large amount of wastewater, known as bone gelatin production waste liquid. If not properly utilized, this wastewater not only leads to a significant waste of social resources but also causes serious pollution to the ecological environment. While classified as "high-chlorine, high-salt, and high-organic-content wastewater" for the gelatin industry, it is rich in various beneficial elements (calcium, magnesium, chlorine, small-molecule active animal proteins, etc.) that improve soil quality and crop growth. Utilizing this wastewater in organic fertilizer production enhances fertilizer efficiency and soil-improving effects. For the gelatin industry, this provides a new product development pathway; for the organic fertilizer industry, it further improves the functionality and effectiveness of organic fertilizer products.

[0003] Currently, there is no existing technology for treating bone gelatin production waste liquid and using it in organic fertilizer production. Therefore, there is a huge market demand and broad application prospects for treating bone gelatin production waste liquid and using it in organic fertilizer production. Summary of the Invention

[0004] The purpose of this invention is to overcome the problems existing in the prior art and provide an organic fertilizer, its preparation method and application. This organic fertilizer has good fertilizer effect and can improve crop yield and agricultural product quality in agricultural production, and has significant market prospects.

[0005] To achieve the above objectives, the first aspect of the present invention provides an organic fertilizer, wherein, on a dry basis, the organic fertilizer contains 0.9-2.5 wt% Ca, 3.1-6.9 wt% Cl, 0.05-0.15 wt% Mg, and 0.25-0.75 wt% small molecule animal protein.

[0006] The second aspect of the present invention provides a method for preparing organic fertilizer, comprising the following steps: concentrating bone gelatin production waste liquid to obtain a concentrated liquid, and mixing the concentrated liquid with raw materials for preparing organic fertilizer; wherein the raw materials for preparing organic fertilizer are livestock manure and / or animal and plant residues.

[0007] The third aspect of this invention provides the application of the organic fertilizer described in the first aspect and / or the organic fertilizer obtained by the preparation method described in the second aspect in agricultural production.

[0008] Through the above technical solution, the organic fertilizer provided by this invention contains 0.9-2.5 wt% Ca, 3.1-6.9 wt% Cl, 0.05-0.15 wt% Mg, and 0.25-0.75 wt% small molecule animal protein. By controlling the Ca, Cl, Mg, and small molecule animal protein contents within specific ranges, and combining the synergistic effects of Ca, Cl, Mg, and small molecule animal protein, the fertilizer efficiency of the organic fertilizer can be effectively improved. Furthermore, it can increase the pelleting rate and gloss of the organic fertilizer, and shorten the disintegration time. When this organic fertilizer is applied to crop growth, it can improve crop yield and quality; moreover, it can improve the soil, reduce soil bulk density, and enhance soil aeration and water and fertilizer retention capacity. Detailed Implementation

[0009] The endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.

[0010] In a first aspect, the present invention provides an organic fertilizer, wherein, on a dry basis, the organic fertilizer contains 0.9-2.5 wt% Ca, specifically 0.9 wt%, 1.5 wt%, 2.0 wt%, 2.5 wt%, or any value between the two aforementioned values; 3.1-6.9 wt% Cl, specifically 3.1 wt%, 4.0 wt%, 4.5 wt%, 5.0 wt%, 5.5 wt%, 6.0 wt%, 6.9 wt%, or any value between the two aforementioned values; 0.05-0.15 wt% Mg, specifically 0.05 wt%, 0.08 wt%, 0.10 wt%, 0.12 wt%, 0.15 wt%, or any value between the two aforementioned values; and 0.25-0.75 wt% small molecule animal protein, specifically 0.25 wt%, 0.5 wt%, 0.75 wt%, or any value between the two aforementioned values.

[0011] During their research, the inventors of this invention unexpectedly discovered that by controlling the Ca, Cl, Mg, and small-molecule animal protein content in organic fertilizers within specific ranges, the synergistic effect of these specific amounts of Ca, Cl, Mg, and small-molecule animal protein can effectively improve the fertilizer efficiency of organic fertilizers. Furthermore, it can increase the pelleting rate and gloss of the organic fertilizers, and shorten their disintegration time. When this organic fertilizer is applied to crop growth, it can increase crop yield and quality; moreover, it can improve soil, reduce soil bulk density, and enhance soil aeration and water and fertilizer retention capacity.

[0012] In this invention, the dry basis of the organic fertilizer refers to the organic fertilizer being stored at a temperature of 50.0 (±2.0)℃ and a vacuum degree of 6.4 × 10⁻⁶. 4 Pa -7.1×10 4 Pa is the amount of water dried in a vacuum oven (the vacuum drying oven was purchased from Shanghai-Heng Scientific Instruments Co., Ltd., DZF series) for 120 (±10) min without any change in mass.

[0013] According to the present invention, the Ca content in organic fertilizer is tested according to the method in GB / T 6436-2018 Determination of Calcium in Feed, the Cl content is tested according to the method in GB 11896-1989 Determination of Chloride in Water by Silver Nitrate Titration, the Mg content is tested according to the method in NY / T 1117-2010 Determination of Calcium, Magnesium, Sulfur and Chlorine Content in Water-Soluble Fertilizers, and the small molecule animal protein content is tested according to the method in GB / T 6432-2018 Determination of Crude Protein in Feed by Kjeldahl Method.

[0014] According to the present invention, preferably, the molecular weight of the small molecule animal protein is ≤10000 Da, more preferably 180-1000 Da, specifically 180 Da, 500 Da, 1000 Da, or any value between the two aforementioned values. The inventors have found that, under this preferred embodiment, when the organic fertilizer contains small molecule animal protein with the above-mentioned molecular weight, it can effectively promote plant root growth, significantly increasing the length of the taproot and the number of lateral roots.

[0015] According to the present invention, preferably, the weight ratio of Ca to small molecule animal protein in the organic fertilizer is 3.5-3.7:1, specifically 3.5:1, 3.6:1, 3.7:1, or any value between the two aforementioned values. The inventors have found that, under this preferred embodiment, by controlling the weight ratio of Ca to small molecule animal protein within the above range, plant root growth can be effectively promoted, with a significant increase in the length of the taproot and the number of lateral roots. This further synergistically promotes the absorption of mineral nutrients such as calcium and magnesium by crops, thereby enhancing the yield-increasing effect of small molecule animal protein on plants.

[0016] According to the present invention, preferably, the weight ratio of Ca, Cl, Mg and small molecule animal protein in the organic fertilizer is 3.5-3.7:9-12:0.16-0.2:1. The inventors have found that, under this preferred embodiment, controlling the weight ratio of Ca, Cl, Mg and small molecule animal protein in the organic fertilizer within the above range, the synergistic effect between Ca, Cl, Mg and small molecule animal protein at specific dosage ratios can further improve the fertilizer efficiency of the organic fertilizer and promote plant growth.

[0017] In this invention, the formulation of the organic fertilizer can be a conventional choice in the art, such as granules, powder, or flakes.

[0018] Secondly, the present invention provides a method for preparing organic fertilizer, comprising the following steps: concentrating bone gelatin production waste liquid to obtain a concentrated liquid, and mixing the concentrated liquid with raw materials for preparing organic fertilizer; wherein the raw materials for preparing organic fertilizer are livestock manure and / or animal and plant residues.

[0019] The preparation method provided by this invention is simple and easy to produce. It can realize the comprehensive utilization of waste liquid from bone gelatin production, utilize the potential economic value of the waste liquid, turn waste into treasure, reduce environmental pollution, and has significant economic benefits.

[0020] In this invention, bone gelatin production waste liquid refers to the final liquid waste obtained after insoluble matter removal, protein degradation, and membrane separation during bone gelatin production. The substances and their contents in different batches of bone gelatin production waste liquid will vary depending on the bone gelatin production process.

[0021] According to the present invention, the solids in the bone gelatin production waste liquid refer to the solid substances obtained after drying the bone gelatin production waste liquid. Preferably, the solids content in the bone gelatin production waste liquid is 5-7 wt%, specifically 5 wt%, 6 wt%, 7 wt%, or any value between the two aforementioned values.

[0022] In this invention, the process of testing the solid content includes: drying the bone gelatin production waste liquid using a drying method to obtain solid substances, and calculating the solid content in the bone gelatin production waste liquid.

[0023] According to the present invention, preferably, the pH of the bone gelatin production waste liquid is 6.5-7, specifically 6.5, 6.6, 6.7, 6.8, 6.9, 7, or any value between the two aforementioned values. The inventors have found that, under this preferred embodiment, the corrosion of the bone gelatin production waste liquid on production equipment can be reduced, extending the service life of the production equipment.

[0024] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the Ca content in the bone gelatin production waste liquid is 1.4-2.1 wt%, specifically 1.4 wt%, 1.6 wt%, 1.8 wt%, 2.1 wt%, or any value between the two aforementioned values.

[0025] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the Cl content in the bone gelatin production waste liquid is 2.9-4.1 wt%, specifically 2.9 wt%, 3.2 wt%, 3.4 wt%, 3.6 wt%, 3.8 wt%, 4.1 wt%, or any value between the two aforementioned values.

[0026] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the Mg content in the bone gelatin production waste liquid is 0.04-0.07wt%, specifically 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, or any value between the two aforementioned values.

[0027] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the content of small molecule animal protein in the bone gelatin production waste liquid is 0.4-0.6 wt%, specifically 0.4 wt%, 0.5 wt%, 0.6 wt%, or any value between the two aforementioned values.

[0028] According to the present invention, preferably, the molecular weight of the small molecule animal protein in the bone gelatin production waste liquid is ≤10000 Da, more preferably 180-1000 Da, specifically 180 Da, 500 Da, 1000 Da, or any value between the aforementioned two values. The inventors have found that, under this preferred embodiment, when the organic fertilizer contains small molecule animal protein with the above-mentioned molecular weight, it can effectively promote plant root growth, significantly increasing the length of the taproot and the number of lateral roots.

[0029] In this invention, the waste liquid from bone gelatin production is concentrated to obtain a concentrated liquid. There are no particular limitations on the concentration method; any conventional method within the art can be used, such as vacuum evaporation, thermal evaporation, or air-drying evaporation. Preferably, the concentration method is vacuum evaporation.

[0030] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the solid content in the concentrate is 30-42 wt%, specifically 30 wt%, 32 wt%, 34 wt%, 36 wt%, 38 wt%, 40 wt%, 42 wt%, or any value between the two aforementioned values.

[0031] According to the present invention, in order to further reduce corrosion of production equipment and extend its service life, preferably, the pH of the concentrate is 6.5-7, specifically 6.5, 6.6, 6.7, 6.8, 6.9, 7, or any value between the two aforementioned values.

[0032] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the Ca content in the concentrate is 8-13 wt%, specifically 8 wt%, 9 wt%, 10 wt%, 11 wt%, 12 wt%, 13 wt%, or any value between the two aforementioned values.

[0033] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the Cl content in the concentrate is 17-25 wt%, specifically 17 wt%, 20 wt%, 25 wt%, or any value between the two aforementioned values.

[0034] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the Mg content in the concentrate is 0.27-0.38 wt%, specifically 0.27 wt%, 0.3 wt%, 0.32 wt%, 0.34 wt%, 0.36 wt%, 0.38 wt%, or any value between the two aforementioned values.

[0035] According to the present invention, in order to further improve the fertilizer efficiency of organic fertilizer, preferably, the content of small molecule animal protein in the concentrate is 2.5-3.6 wt%, specifically 2.5 wt%, 2.8 wt%, 3 wt%, 3.2 wt%, 3.4 wt%, 3.6 wt%, or any value between the two aforementioned values.

[0036] In this invention, the Ca content in the waste liquid and concentrate of bone gelatin production is tested according to the method in GB / T 6436-2018 Determination of Calcium in Feed, the Cl content is tested according to the method in GB 11896-1989 Determination of Chloride in Water by Silver Nitrate Titration, the Mg content is tested according to the method in NY / T 1117-2010 Determination of Calcium, Magnesium, Sulfur and Chlorine Content in Water-Soluble Fertilizers, and the small molecule animal protein content is tested according to the method in GB / T 6432-2018 Determination of Crude Protein in Feed by Kjeldahl Method.

[0037] In this invention, livestock manure can be a conventional choice in organic fertilizer production, such as sheep manure, cow manure, and pig manure. To further improve the fertilizer efficiency of the organic fertilizer, preferably, the livestock manure is fermented and decomposed livestock manure material (hereinafter referred to as decomposed material), and more preferably, fermented and decomposed sheep manure material. Fermented and decomposed sheep manure material is a decomposed raw material obtained using conventional high-temperature fermentation methods during the organic fertilizer production process.

[0038] In this invention, the animal and plant residues can be a conventional choice in organic fertilizer production, such as plant straw. To further improve the fertilizer efficiency of the organic fertilizer, preferably, the animal and plant residues are fermented and decomposed materials.

[0039] According to the present invention, the raw materials for preparing organic fertilizer can be livestock manure, animal and plant residues, or a mixture of livestock manure and animal and plant residues. When the raw materials for preparing organic fertilizer are livestock manure and animal and plant residues, the weight ratio of livestock manure to animal and plant residues is preferably 1:0.5-5.

[0040] According to the present invention, preferably, the weight ratio of the concentrate to the organic fertilizer preparation raw materials is 1:3-5, specifically 1:3, 1:4, 1:5, or any value between the two aforementioned values. The inventors have found that, under this preferred embodiment, the synergistic effect of the concentrate and the organic fertilizer preparation raw materials within the above weight range improves the fertilizer efficiency of the organic fertilizer and promotes plant growth.

[0041] In this invention, organic fertilizer can be obtained by mixing concentrated liquid with raw materials for organic fertilizer preparation. There are no particular limitations on the formulation of organic fertilizer, which can be powder or granules.

[0042] In this invention, organic fertilizer can be made into granules. Preferably, granulation additives are added to the concentrate before the concentrate is mixed with the raw materials for preparing organic fertilizer.

[0043] According to the present invention, in order to further improve the granulation effect of organic fertilizer, preferably, the granulation auxiliary material is selected from at least one of water, amino acid aqueous solution, humic acid aqueous solution and microbial functional bacteria solution.

[0044] In this invention, when the granulation auxiliary material is water, the water quality meets the "GB 5084-2021 Standard for Irrigation Water Quality"; when the granulation auxiliary material is an amino acid aqueous solution, the amino acid content in the granulation auxiliary material is preferably 20-70 wt%; when the granulation auxiliary material is other raw materials, the producer can adjust the proportion and content according to its needs.

[0045] According to the present invention, in order to further improve the granulation effect of organic fertilizer, preferably, the weight ratio of the granulation auxiliary material to the concentrate is 1:3-5, specifically 1:3, 1:4, 1:5, or any value between the two aforementioned values.

[0046] In this invention, the organic fertilizer granulation can be carried out using conventional granulation methods selected in organic fertilizer production, such as drum granulation, disc granulation, or spray granulation.

[0047] According to the present invention, preferably, the method further includes: drying the mixed material to obtain the organic fertilizer.

[0048] According to the present invention, drying can be carried out using conventional drying methods selected in the art, such as heat drying, forced-air drying, etc. Exemplarily, the drying process includes: using a rotary drum dryer, by introducing heat energy into the dryer drum to raise the temperature inside the drum to 150±10℃. The temperature can be adjusted according to the characteristics of different materials, vaporizing the moisture in the material to remove the moisture, which is then carried out of the dryer with the air. To further improve the fertilizer efficiency of the organic fertilizer, preferably, the drying conditions include at least: the moisture content of the organic fertilizer is 20-30 wt%, specifically 20 wt%, 25 wt%, 30 wt%, or any value between the aforementioned two values.

[0049] According to a particularly preferred embodiment of the present invention, a method for preparing organic fertilizer is provided, the method comprising the following steps:

[0050] The waste liquid from bone gelatin production is concentrated to obtain a concentrated liquid. The concentrated liquid and the raw materials for organic fertilizer preparation are mixed at a weight ratio of 1:3-5. The mixed material is dried to obtain an organic fertilizer with a moisture content of 20-30 wt%.

[0051] The waste liquid from bone gelatin production contains 5-7 wt% solids, has a pH of 6.5-7, 1.4-2.1 wt% Ca, 2.9-4.1 wt% Cl, 0.04-0.07 wt% Mg, and 0.4-0.6 wt% small molecule animal protein; the molecular weight of the small molecule animal protein is 180-1000 Da. The concentrated liquid contains 30-42 wt% solids, has a pH of 6.5-7, 8-13 wt% Ca, 17-25 wt% Cl, 0.27-0.38 wt% Mg, and 2.5-3.6 wt% small molecule animal protein. The raw materials for organic fertilizer preparation are livestock manure and / or animal and plant residues, where the livestock manure is fermented and decomposed sheep manure, and the animal and plant residues are fermented and decomposed materials.

[0052] In the preferred embodiments described above, the method for preparing organic fertilizer can increase the specific gravity, pelleting rate, and gloss of organic fertilizer powder and granules, and shorten the disintegration time, thereby shortening the production cycle of organic fertilizer and improving its fertilizer efficiency and sales radius.

[0053] Thirdly, the present invention provides the application of the organic fertilizer described in the first aspect and / or the organic fertilizer obtained by the preparation method described in the second aspect in agricultural production.

[0054] In this invention, when the aforementioned organic fertilizer is applied to agricultural production, it can improve the soil. Regarding soil improvement, this organic fertilizer can promote the formation of stable soil aggregates, reduce soil bulk density, and improve soil aeration and water and fertilizer retention capacity, thereby improving the soil.

[0055] In this invention, when the aforementioned organic fertilizer is applied to agricultural production, it can improve the yield and quality of crops. The crops can be conventional crops in the art, such as melons, fruits, vegetables, and grains. Preferably, the crops are selected from at least one of corn, sugar beets, sunflowers, tomatoes, cucumbers, lettuce, leeks, and chili peppers; more preferably, at least one of corn, sugar beets, and sunflowers.

[0056] The present invention will be described in detail below with reference to embodiments, but this does not limit the scope of the invention.

[0057] In the following examples, the fermented and decomposed sheep manure was purchased from Wuyuan County Wofeng Biotechnology Co., Ltd., with technical specifications of organic matter ≥30% and total nutrients ≥4%; the amino acid aqueous solution was purchased from Xuzhou Weitian Technology Co., Ltd., with technical specifications of free amino acids ≥350g / L, and the product type was amino acid stock solution (acid hydrolysis of animal source), 350g / L low chlorine type. Unless otherwise specified, all other raw materials or reagents were conventional commercially available products.

[0058] Example 1

[0059] This embodiment illustrates the preparation of organic fertilizer powder F1 according to the method of the present invention.

[0060] (1) The gelatin production waste liquid (solid concentration 5wt%, pH 6.9, Ca content 1.47wt%, Cl content 2.90wt%, Mg content 0.05wt%, small molecule animal protein content 0.43wt%) was concentrated to obtain calcium peptide concentrate, at which time the solid concentration in the calcium peptide concentrate was 30wt%.

[0061] (2) The calcium peptide concentrate obtained in step (1) is mixed with sheep manure fermented compost to obtain a mixture; wherein, the calcium peptide concentrate is by weight and the sheep manure fermented compost is by dry basis, and the weight percentage of calcium peptide concentrate to sheep manure fermented compost is 20:80.

[0062] (3) The mixture obtained in step (2) is dried to obtain organic fertilizer powder F1 with a moisture content of 30 wt%.

[0063] Example 2

[0064] This embodiment illustrates the preparation of organic fertilizer powder F2 according to the method of the present invention.

[0065] (1) The gelatin production waste liquid (solid concentration 6wt%, pH 6.9, Ca content 1.77wt%, Cl content 3.48wt%, Mg content 0.05wt%, small molecule animal protein content 0.52wt%) was concentrated to obtain calcium peptide concentrate, at which time the solid concentration in the calcium peptide concentrate was 35wt%.

[0066] (2) The calcium peptide concentrate obtained in step (1) is mixed with sheep manure fermented compost to obtain a mixture; wherein, the calcium peptide concentrate is by weight and the sheep manure fermented compost is by dry basis, and the weight percentage of calcium peptide concentrate to sheep manure fermented compost is 20:80.

[0067] (3) The mixture obtained in step (2) is dried to obtain organic fertilizer powder F2 with a moisture content of 30 wt%.

[0068] Example 3

[0069] This embodiment illustrates the preparation of organic fertilizer powder F3 according to the method of the present invention.

[0070] (1) The gelatin production waste liquid (solid concentration 7wt%, pH 6.9, Ca content 2.06wt%, Cl content 4.07wt%, Mg content 0.06wt%, small molecule animal protein content 0.6wt%) was concentrated to obtain calcium peptide concentrate, at which time the solid concentration in the calcium peptide concentrate was 40wt%.

[0071] (2) The calcium peptide concentrate obtained in step (1) is mixed with sheep manure fermented compost to obtain a mixture; wherein, the calcium peptide concentrate is by weight and the sheep manure fermented compost is by dry basis, and the weight percentage of calcium peptide concentrate to sheep manure fermented compost is 20:80.

[0072] (3) The mixture obtained in step (2) is dried to obtain organic fertilizer powder F3 with a moisture content of 30 wt%.

[0073] Example 4

[0074] This embodiment illustrates the preparation of organic fertilizer granules K1 according to the method of the present invention.

[0075] (1) The gelatin production waste liquid (solid concentration 5wt%, pH 6.9, Ca content 1.47wt%, Cl content 2.9wt%, Mg content 0.05wt%, small molecule animal protein content 0.43wt%) was concentrated to obtain calcium peptide concentrate, at which time the solid concentration in the calcium peptide concentrate was 30wt%.

[0076] (2) Mix the calcium peptide concentrate obtained in step (1) with water to obtain fertilizer granulation solution; by weight, the weight percentage of calcium peptide concentrate to water is 80:20.

[0077] (3) Granulate the fermented and decomposed sheep manure with the fertilizer granulation liquid obtained in step (2) to obtain organic fertilizer granules K1 with a water content of 20wt%; wherein, the granulation liquid is by weight and the fermented and decomposed sheep manure is by dry basis, and the weight percentage of the granulation liquid to the fermented and decomposed sheep manure is 25:80.

[0078] Example 5

[0079] This embodiment illustrates the preparation of organic fertilizer granules K2 according to the method of the present invention.

[0080] (1) The gelatin production waste liquid (solid concentration 6wt%, pH 6.9, Ca content 1.77wt%, Cl content 3.48wt%, Mg content 0.05wt%, small molecule animal protein content 0.52wt%) was concentrated to obtain calcium peptide concentrate, at which time the solid concentration in the calcium peptide concentrate was 35wt%.

[0081] (2) The calcium peptide concentrate obtained in step (1) is mixed with an amino acid aqueous solution (pH 4±0.2) to obtain fertilizer granulation solution; by weight, the weight percentage of calcium peptide concentrate to amino acid aqueous solution is 80:20.

[0082] (3) Granulate the fermented and decomposed sheep manure with the fertilizer granulation liquid obtained in step (2) to obtain organic fertilizer granules K2 with a water content of 20wt%; wherein, the granulation liquid is by weight and the fermented and decomposed sheep manure is by dry basis, and the weight percentage of the granulation liquid to the fermented and decomposed sheep manure is 25:80.

[0083] Example 6

[0084] This embodiment illustrates the preparation of organic fertilizer granules K3 according to the method of the present invention.

[0085] (1) The gelatin production waste liquid (solid concentration 6wt%, pH 6.9, Ca content 1.77wt%, Cl content 3.48wt%, Mg content 0.05wt%, small molecule animal protein content 0.52wt%) was concentrated to obtain calcium peptide concentrate, at which time the solid concentration in the calcium peptide concentrate was 40wt%.

[0086] (2) Mix the calcium peptide concentrate obtained in step (1) with water to obtain fertilizer granulation solution; by weight, the weight percentage of calcium peptide concentrate to water is 80:20.

[0087] (3) Granulate the fermented and decomposed sheep manure with the fertilizer granulation liquid obtained in step (2) to obtain organic fertilizer granules K3 with a water content of 20wt%; wherein, the granulation liquid is by weight and the fermented and decomposed sheep manure is by dry basis, and the weight percentage of the granulation liquid to the fermented and decomposed sheep manure is 25:80.

[0088] Example 7

[0089] This embodiment illustrates the preparation of organic fertilizer powder F4 according to the method of the present invention.

[0090] Organic fertilizer powder product F4 was prepared according to the method of Example 1, except that in step (2), the weight percentage of calcium peptide concentrate and sheep manure fermentation compost was replaced with 10:90.

[0091] Example 8

[0092] This embodiment illustrates the preparation of organic fertilizer granules K4 according to the method of the present invention.

[0093] Organic fertilizer powder product K4 was prepared according to the method of Example 4, except that in step (2), the weight percentage of calcium peptide concentrate to water was replaced with 90:10.

[0094] Example 9

[0095] This embodiment illustrates the preparation of organic fertilizer powder F5 according to the method of the present invention.

[0096] (1) The gelatin production waste liquid (solid concentration 5wt%, pH 6.9, Ca content 1.47wt%, Cl content 2.9wt%, Mg content 0.05wt%, small molecule animal protein content 0.43wt%) was concentrated to obtain calcium peptide concentrate, at which time the solid concentration in the calcium peptide concentrate was 30wt%.

[0097] (2) The calcium peptide concentrate obtained in step (1) is mixed with sheep manure fermentation and composting material to obtain organic fertilizer powder F5 with a water content of 40 wt%; wherein, the calcium peptide concentrate is by weight and the sheep manure fermentation and composting material is by dry basis, and the weight percentage of calcium peptide concentrate to sheep manure fermentation and composting material is 20:80.

[0098] Comparative Example 1

[0099] Organic fertilizer powder DF1 with a moisture content of 30wt% is obtained by directly drying fermented and decomposed sheep manure.

[0100] Comparative Example 2

[0101] Organic fertilizer granules DK1 with a moisture content of 20wt% are obtained by granulating fermented and decomposed sheep manure with clean water and then drying it.

[0102] Comparative Example 3

[0103] Organic fertilizer powder DF2 was prepared according to the method of Example 1, except that in step (1), the gelatin production waste liquid was replaced with gelatin production waste liquid with added sodium carbonate to remove calcium ions (solid concentration 5wt%, pH 6.9, Ca content 0wt%, Cl content 2.9wt%, Mg content 0.05wt%, small molecule animal protein content 0.52wt%).

[0104] Comparative Example 4

[0105] Organic fertilizer granules DK2 were prepared according to the method of Example 4, except that in step (1), calcium peptide concentrate was obtained by concentration, and the solids concentration in the calcium peptide concentrate was replaced with 20 wt%.

[0106] Test Example 1

[0107] The pH of the calcium peptide concentrates obtained in Examples 1-9 and Comparative Examples 1-4 were tested, and the contents of solids (wt%), Ca (wt%), Cl (wt%), Mg (wt%) and small molecule animal protein (wt%) in the calcium peptide concentrates and organic fertilizers were tested. The results are shown in Table 1.

[0108] Table 1

[0109]

[0110] Test Example 2

[0111] This test case is used to detect the physical and technical properties of organic fertilizers (powder).

[0112] The physical and technical properties (specific gravity) of the organic fertilizers F1, F2, F3, F4, F5, DF1, and DF2 prepared in Examples 1-3, Example 7, Example 9, Comparative Example 1, and Comparative Example 3 were tested, and the results are shown in Table 2.

[0113] Specific gravity (ω) is the ratio of the weight of fertilizer to its volume, that is, the weight per unit volume of fertilizer. The method for determining specific gravity includes: taking a certain weight of fertilizer M and placing it in a container, pouring in a known volume of water V1 (higher than the fertilizer), and reading its volume V2. ω = M / (V2-V1).

[0114] Table 2

[0115] Product Number F1 F2 F3 F4 F5 DF1 DF2 proportion 0.87 0.87 0.88 0.86 0.88 0.85 0.85 Increase / decrease (%) 2.35 2.35 3.53 1.17 3.53 - 0

[0116] As can be seen from the results in Table 2, compared with Comparative Example 1 (DF1) and Comparative Example 3 (DF2), the organic fertilizer powders (F1-F5) prepared by the method provided by the present invention in Examples 1-3, 7 and 9 can effectively increase the specific gravity and reduce the volume of organic fertilizer, thereby increasing the sales radius of organic fertilizer.

[0117] Test Example 3

[0118] This test case is used to detect the physical and technical indicators of organic fertilizer (granules).

[0119] The physical and technical properties (specific gravity, pelleting rate, gloss, and disintegration time) of the organic fertilizer granules K1, K2, K3, K4, DK1, and DK2 prepared in Examples 4-6, Example 8, Comparative Example 2, and Comparative Example 4 were tested, and the results are shown in Table 3.

[0120] Pelletization rate refers to the ratio of the yield of commercial granular organic fertilizer to the amount of raw material input per unit time. The higher the pelletization rate, the greater the production volume per unit time and the lower the production cost. The calculation formula is: Pelletization rate (%) = (W1 / W) × 100; where W1 is the weight of the organic fertilizer granules produced per unit time, and W is the amount of raw material input per unit time.

[0121] Gloss refers to a physical quantity that evaluates the ability of a material surface to reflect light under a set of geometrically defined conditions. Higher gloss indicates better marketability of granular fertilizer. Gloss data for organic fertilizer granules was obtained using a gloss measuring instrument, specifically the YG60 gloss measuring instrument manufactured by Guangdong Sanenshi Technology Co., Ltd.

[0122] Disintegration time: Disintegration time refers to the time it takes for granular fertilizer to break down, disintegrate, or decompose in the soil under the influence of changes in soil moisture and other conditions. The shorter the disintegration time, the better the fertilizer effect. Disintegration time is determined using an indoor hydrolysis observation method: a certain amount of granular organic fertilizer is placed in a container, and the same volume of water is added. The time required for all the granules to disintegrate and dissolve is measured at room temperature (25℃).

[0123] Table 3

[0124] Product Number K1 K2 K3 K4 DK1 DK2 proportion 0.93 0.94 0.95 0.94 0.92 0.92 Ball formation rate (%) 91.51 91.62 92.67 90.15 87.61 88.52 gloss 0.2 0.2 0.2 0.2 0.0 0.1 Disintegration time / h 4.5 4.45 4.45 5 10 6.25

[0125] As can be seen from the results in Table 3, compared with Comparative Example 2 (DK1) and Comparative Example 4 (DK2), the organic fertilizer granules (K1-K4) prepared by the method provided by the present invention in Examples 4-6 and Example 8 can not only increase the specific gravity of organic fertilizer, reduce the volume of organic fertilizer, and increase the sales radius of the product; but also significantly increase the pelleting rate of organic fertilizer, shorten the production cycle, and increase the production output; improve the gloss of organic fertilizer and improve the marketability of the product; and reduce the disintegration time of organic fertilizer and increase the fertilizer efficiency of the product.

[0126] Test Example 4

[0127] This test case illustrates the effectiveness of organic fertilizers.

[0128] To verify the fertilizer efficacy of the organic fertilizer produced by the technical method of this invention, field fertilizer efficacy comparison tests were conducted in Wayao Village and Taipingzhuang Village of Tumote Right Banner, Baotou City, Inner Mongolia; Wulanjijiu Village of Jiuyuan District, Baotou City, Inner Mongolia; Zhongtan Sugar Beet Base of Wuyuan County, Bayannur City, Inner Mongolia; Dongshizhou Village of Tumote Left Banner, Hohhot City, Inner Mongolia; and Tiemaogedan Village of Taohai Town, Wuyuan County, Inner Mongolia.

[0129] Experiment 1

[0130] 1.1 Basic Information of the Experimental Site

[0131] 1.1.1 Test location: Wayaocun Village, Tumote Right Banner, Baotou City, Inner Mongolia

[0132] 1.1.2 Landform type: Alluvial plain

[0133] 1.1.3 Soil type and texture: Chestnut soil; Medium loam

[0134] 1.1.4 Test conditions: Open air

[0135] 1.1.5 Implementing Unit: Inner Mongolia Dongbao Datian Biotechnology Co., Ltd.

[0136] 1.1.6 Test period: April 25, 2023 – October 15, 2023

[0137] 1.2 Test Materials

[0138] Test crop: Maize

[0139] 1.3 Experimental Design

[0140] 1.3.1 Experimental Treatments: Fertilization was carried out on April 25, 2023, with four treatments: Treatment 1 (organic fertilizer powder DF1), Treatment 2 (organic fertilizer powder F1), Treatment 3 (organic fertilizer powder F2), and Treatment 4 (organic fertilizer powder F3) (Treatment 1 served as a control). These treatments were applied to eight plots. The application rate was 300 kg / mu (approximately 150 kg / acre), and the application method was basal application. Except for the treatments described, the types and quantities of other basal fertilizers (chemical fertilizers) and topdressings were consistent.

[0141] 1.3.2 Field Design: The plot is 10.6m long and 63m wide, with a net area of ​​668m². 2 (equivalent to 1 mu).

[0142] Repeat twice, setting up protected rows. A total of 8 plots were set up, randomly arranged, and the fertilizer application and amount for each plot are shown in Table 4.

[0143] Table 4

[0144] project crop Fertilization treatment Fertilizer application rate Community 1 corn Process 1 300kg / mu Community 2 corn Process 2 300kg / mu Community 3 corn Process 1 300kg / mu Community 4 corn Process 2 300kg / mu Community 5 corn Process 3 300kg / mu Community 6 corn Process 4 300kg / mu Community 7 corn Process 3 300kg / mu Community 8 corn Process 4 300kg / mu

[0145] 1.4 Test Results

[0146] 1.4.1 Effects on soil physicochemical properties

[0147] Data collection time: April 25 and October 15, before fertilization.

[0148] Collection method: Before fertilization and before harvest, three sampling points were randomly selected in each plot to collect soil samples in the 0-20cm and 20-40cm ranges. Soil bulk density and soil nutrient content were tested. The test results are shown in Tables 5 and 6.

[0149] Soil bulk density, also known as dry bulk density or soil pseudo-specific gravity, is the ratio of the mass of a given volume of soil (including soil particles and interparticle pores) after drying to its volume before drying. It is determined using the ring sampler method one cycle after fertilization (before crop harvest). The calculation formula is: Soil bulk density (ρ... b = Mass of dried soil sample / Volume of ring cutter

[0150] Table 5

[0151]

[0152]

[0153] Table 6

[0154]

[0155]

[0156] The results in Tables 5 and 6 show that, in the same field soil improvement and fertilizer effect comparison test, organic fertilizer powders F1, F2, F3 and DF1 were tested. The results showed that, compared with organic fertilizer powder DF1, organic fertilizer powders F1, F2 and F3 could further improve soil structure, improve soil properties, reduce soil salinity in the topsoil, and reduce soil bulk density.

[0157] 1.4.2 Effects of different treatments on maize growth and yield

[0158] The differences in field growth among the treatments in the eight plots were observed, and the results are shown in Table 7.

[0159] Table 7

[0160] deal with Plant height (cm) Ear position (cm) Moisture content (wt%) Weight of 100 grains (g) Process 1 231.62 116.5 34.1 37.8 Process 2 228.6 115.2 34.6 38.28 Process 3 225.3 116.2 34.5 38.5 Process 4 223.5 116.5 34.5 38.9

[0161] After the corn matured, the yield was determined by the "harvesting method". The corn harvesting area was 6 square meters (10m*0.6m). Two representative sampling points were taken from each treatment in the sub-district. The corn was threshed and dried indoors (the moisture content of the corn was below 14wt%) and weighed. The yield per mu was calculated. The results are shown in Table 8.

[0162] Table 8

[0163]

[0164] As can be seen from Table 8, the treatments had little impact on maize growth, but the organic fertilizer powders F1, F2, and F3 showed a significant increase in 100-grain weight compared to organic fertilizer powder DF1, which could further improve maize yield.

[0165] Analysis of variance (also known as one-way ANOVA) was used to study the variability in yield, and the results are shown in Table 9.

[0166] Table 9

[0167]

[0168] As can be seen from the table above, all different treatment samples showed significant differences in yield (p<0.05), which means that different treatment samples had different yields.

[0169] Specific analysis shows that the treatments were significant at the 0.05 level for yield (F = 5.799, p = 0.011). The comparison of the differences shows that the mean scores of the groups with more obvious differences are "F1 > FK; F2 > DF1; F3 > DF1". All different treatment samples showed significant differences in yield.

[0170] The experimental results showed that when the same field fertilizer effect comparison test was conducted on organic fertilizer powders F1, F2, F3 and DF1, the results showed that organic fertilizers F1, F2 and F3 significantly increased the 100-grain weight and significantly increased the yield of corn compared with organic fertilizer powder DF1.

[0171] 2 Experiments 2

[0172] 2.1 Basic Information of the Experimental Site

[0173] 2.1.1 Test location: Taipingzhuang Village, Tumote Right Banner, Baotou City, Inner Mongolia

[0174] 2.1.2 Landform type: Alluvial plain

[0175] 2.1.3 Soil type and texture: saline-alkali soil, medium loam

[0176] 2.1.4 Test conditions: Open air

[0177] 2.1.5 Implementing Unit: Inner Mongolia Chulechuan Sugar Industry

[0178] 2.1.6 Test period: April 20, 2022 – October 8, 2023

[0179] 2.2 Test Materials

[0180] Test crop: sugar beet

[0181] 2.3 Experimental Design

[0182] 2.3.1 Experimental treatments: On April 25, 2023, a total of four treatments were set up. Treatment 1 was organic fertilizer granules DK1, treatment 2 was organic fertilizer granules DK2, treatment 3 was organic fertilizer granules K1, and treatment 4 was organic fertilizer granules K4. Except for the treatments, the varieties and quantities of other base fertilizers (chemical fertilizers) and top dressings were the same.

[0183] 2.3.2 Field Design: The field is 106m long and 63m wide, with a net area of ​​668m². 2 (equivalent to 1 mu)

[0184] Repeat three times, setting up a protection row. A total of 12 cells were set up: 3 cells for treatment 1, 3 cells for treatment 2, 3 cells for treatment 3, and the remaining 3 cells for treatment 4. The fertilizer application and amount for each cell are shown in Table 10.

[0185] Table 10

[0186] project crop Fertilization treatment Fertilizer application rate Community 1 beet Process 1 100kg / mu Community 2 beet Process 2 100kg / mu Community 3 beet Process 1 100kg / mu Community 4 beet Process 2 100kg / mu Community 5 beet Process 1 100kg / mu Community 6 beet Process 2 100kg / mu Community 7 beet Process 3 100kg / mu Community 8 beet Process 4 100kg / mu Community 9 beet Process 3 100kg / mu Community 10 beet Process 4 100kg / mu Community 11 beet Process 3 100kg / mu Community 12 beet Process 4 100kg / mu

[0187] 2.4 Test Results

[0188] 2.4.1 Impact on Soil

[0189] Data collection time: April 25 and October 8, before fertilization.

[0190] Collection method: Before fertilization and before harvest, three sampling points were randomly selected from each plot to collect soil samples in the 0-20cm and 20-40cm depth ranges, and the soil nutrients and bulk density were tested. The test results are shown in Tables 11 and 12.

[0191] Table 11

[0192]

[0193]

[0194] Table 12

[0195]

[0196] The experimental results showed that, comparing the nutrient content and soil bulk density before and after using organic fertilizer granules K1 and K4 and organic fertilizer granules DK1 and DK2, the overall soil nutrient content did not change significantly, the soil pH increased slightly, the soil salinity decreased, and the soil bulk density decreased significantly. Moreover, the soil bulk density was lower when using organic fertilizer granules K1 and K4 compared to when using organic fertilizer granules DK1 and DK2.

[0197] 2.4.2 Effects of different treatments on sugar beet yield and quality

[0198] Before the sugar beets matured and were harvested, yield was determined. The initial harvest area was 6 square meters (10m * 0.6m). Three representative sampling points were taken for each treatment. According to the cutting standards for raw sugar beets, stems, leaves, lateral roots, hairy roots, and tail roots with a diameter of less than 1 cm were removed. The soil was scraped off, and the weight of the tubers was weighed to calculate the average weight per plant. The yield per acre was calculated, and the sugar content was measured using a saccharimeter. The results are shown in Table 13.

[0199] Table 13

[0200]

[0201]

[0202] The experimental results showed that, when the same field fertilizer effect comparison test was conducted on organic fertilizer granules K1, K4, DK1, and DK2, it was found that, compared with organic fertilizer granule DK1, the use of organic fertilizer granules K1 and K4 increased the yield of sugar beets by 8.79% and 6.90%, respectively, and the sugar content increased by 0.94% and 0.58%, respectively.

[0203] A field experiment comparing the fertilizer effects of organic fertilizer granules K1, K4, DK1, and DK2 was conducted. The results showed that organic fertilizer granules K1 and K4, compared with organic fertilizer granules DK1 and DK2, could improve the soil and further increase the yield and quality of sugar beets.

[0204] 3 Experiments 3

[0205] 3.1 Basic Information of the Experimental Site

[0206] 3.1.1 Test location: Zhongtan sugar beet base, Wuyuan County, Bayannur City, Inner Mongolia

[0207] 3.1.2 Landform type: Alluvial plain

[0208] 3.1.3 Soil type and texture: Chestnut soil; Medium loam

[0209] 3.1.4 Test conditions: Open air

[0210] 3.1.5 Implementing Unit: Inner Mongolia Zhongtan Sugar Industry Co., Ltd.

[0211] 3.1.6 Test period: April 28, 2023 – October 10, 2023

[0212] 3.2 Test Materials

[0213] Test crop: sugar beet

[0214] 3.3 Experimental Design

[0215] 3.3.1 Experimental treatments: Two treatments were set up on April 28, 2023. Treatment 1 was organic fertilizer granules DK1; treatment 2 was organic fertilizer granules K2. Except for the contents of this treatment, the varieties and quantities of other base fertilizers (chemical fertilizers) and top dressings were the same.

[0216] 3.3.2 Field Design: The field is 106m long and 63m wide, with a net area of ​​668m². 2 (equivalent to 1 mu)

[0217] Repeat three times, setting up a protected row. A total of 6 cells were set up, with 3 cells undergoing treatment 1 and the other 3 cells undergoing treatment 2. The fertilizer applied and the amount of fertilizer applied to each cell are shown in Table 14.

[0218] Table 14

[0219] project crop Fertilization treatment Fertilizer application rate Community 1 beet Process 1 100kg / mu Community 2 beet Process 2 100kg / mu Community 3 beet Process 1 100kg / mu Community 4 beet Process 2 100kg / mu Community 5 beet Process 1 100kg / mu Community 6 beet Process 2 100kg / mu

[0220] 3.4 Test Results

[0221] 3.4.1 Impact on Soil

[0222] Data collection time: April 20 and October 8, before fertilization.

[0223] Collection method: Before fertilization and before harvest, three sampling points were randomly selected from each plot to collect soil samples in the 0-20cm and 20-40cm depth ranges, and the soil nutrients and bulk density were tested. The test results are shown in Tables 15 and 16.

[0224] Table 15

[0225]

[0226] Table 16

[0227]

[0228] The experimental results showed that, compared with organic fertilizer granules K2 and DK1, the overall soil nutrient changes were not significant, the soil pH increased slightly, the total salt level decreased significantly, and the soil bulk density decreased.

[0229] 3.4.2 Effects of different treatments on sugar beet yield and quality

[0230] Before the sugar beets matured and were harvested, yield was measured. The harvesting area was 16 square meters (26m*0.6m). According to the cutting standard of raw sugar beets, the stems, leaves, lateral roots, hairy roots and tail roots with a diameter of less than 1 cm were removed. The soil was scraped off, the weight of the tubers was weighed, the average weight of a single plant was calculated, and the yield per mu of sugar beets was calculated. The sugar content was tested using a saccharimeter. The results are shown in Table 17.

[0231] Table 17

[0232]

[0233] The experimental results showed that, in the same field fertilizer effect comparison test between organic fertilizer granules K2 and DK1, organic fertilizer granules K2 increased sugar beet yield by 21.25% and sugar content by 1.59% compared with organic fertilizer granules DK1.

[0234] A field experiment comparing the fertilizer effects of organic fertilizer granules K2 and DK1 showed that organic fertilizer granules K2 could further improve the yield and quality of sugar beets compared to organic fertilizer DK1.

[0235] Experiment 4

[0236] 4. Basic Information of the Experimental Site

[0237] 4.1.1 Test Location: Wulanjijiu Village, Jiuyuan District, Baotou City, Inner Mongolia

[0238] 4.1.2 Landform type: Alluvial plain

[0239] 4.1.3 Soil type and texture: Chestnut soil; Light loam

[0240] 4.1.4 Test conditions: Open air

[0241] 4.1.5 Implementing Unit: Inner Mongolia Chulechuan Sugar Industry

[0242] 4.1.6 Test period: April 25, 2022 – October 5, 2022

[0243] 4.2 Test Materials

[0244] Test crop: sugar beet

[0245] 4.3 Experimental Design

[0246] 4.3.1 Experimental treatments: Two treatments were set up on April 25, 2023. Treatment 1 was organic fertilizer granules DK1; treatment 2 was organic fertilizer granules K3. Except for the contents of this treatment, the varieties and quantities of other base fertilizers (chemical fertilizers) and top dressings were the same.

[0247] 4.3.2 Field Design: The field is 106m long and 63m wide, with a net area of ​​668m². 2 (equivalent to 1 mu)

[0248] Repeat three times, setting up a protected row. A total of 6 cells were set up, with 3 cells undergoing treatment 1 and the other 3 cells undergoing treatment 2. The fertilizer applied and the amount of fertilizer applied to each cell are shown in Table 18.

[0249] Table 18

[0250] project crop Fertilization treatment Fertilizer application rate Community 1 beet Process 1 100kg / mu Community 2 beet Process 2 100kg / mu Community 3 beet Process 1 100kg / mu Community 4 beet Process 2 100kg / mu Community 5 beet Process 1 100kg / mu Community 6 beet Process 2 100kg / mu

[0251] 4.4 Test Results

[0252] 4.4.1 Impact on Soil

[0253] Data collection time: April 25 and October 8, before fertilization.

[0254] Collection method: Before fertilization and before harvest, three sampling points were randomly selected from each plot to collect soil samples in the 0-20cm and 20-40cm depth ranges, and the soil nutrients and bulk density were tested. The test results are shown in Tables 19 and 20.

[0255] Table 19

[0256]

[0257] Table 20

[0258]

[0259]

[0260] The experimental results showed that, compared with organic fertilizer granules K2 and DK1, the overall soil nutrient changes were not significant, the soil pH increased slightly, the total salt level decreased significantly, and the soil bulk density decreased.

[0261] 4.4.2 Effects of different treatments on sugar beet yield and quality

[0262] Before the sugar beets matured and were harvested, yield was measured. The harvesting area was 6 square meters (10m*0.6m). According to the cutting standard of raw sugar beets, the stems, leaves, lateral roots, hairy roots and tail roots with a diameter of less than 1 cm were removed. The soil was scraped off, the weight of the tubers was weighed, the average weight of a single plant was calculated, and the yield per mu of sugar beets was calculated. The sugar content was tested using a saccharimeter. The results are shown in Table 21.

[0263] Table 21

[0264]

[0265] The experimental results showed that, in the same field fertilizer effect comparison test between organic fertilizer granules K3 and DK1, organic fertilizer granules K3 increased sugar beet yield by 7.35% and sugar content by 1.12% compared with organic fertilizer granules DK1.

[0266] A field experiment comparing the fertilizer effects of organic fertilizer granules K3 and DK1 showed that organic fertilizer granules K3 could further improve the yield and quality of sugar beets compared to organic fertilizer granules DK1.

[0267] 5 trials 5

[0268] 5.1 Basic Information of the Test Site

[0269] 5.1.1 Test Location: Dongshizhou Village, Tumote Left Banner, Hohhot City, Inner Mongolia

[0270] 5.1.2 Landform type: Alluvial plain

[0271] 5.1.3 Soil type and texture: Chestnut soil; medium to heavy loam.

[0272] 5.1.4 Test conditions: Open air

[0273] 5.1.5 Implementing Entity: Inner Mongolia Dongbao Datian Biotechnology Co., Ltd.

[0274] 5.1.6 Test period: April 10, 2023 – September 30, 2023

[0275] 5.2 Test Materials

[0276] Test crop: Maize

[0277] 5.3 Experimental Design

[0278] 5.3.1 Experimental Treatments: Fertilization was carried out on May 1, 2023, with four treatments: Treatment 1 was organic fertilizer granules DK1, Treatment 2 was organic fertilizer granules K1, Treatment 3 was organic fertilizer granules K2, and Treatment 4 was organic fertilizer granules K3 (Treatment 1 served as the control). These were applied to eight plots. The application rate was 80 kg / mu for all treatments, and the application method was basal application. Except for the treatments described, the types and quantities of other basal fertilizers (chemical fertilizers) and topdressings were consistent.

[0279] 5.3.2 Field Design: The field area is 10.6m long and 63m wide, with a net area of ​​668m². 2 (equivalent to 1 mu)

[0280] Repeat twice, setting up protected rows. A total of 8 plots were set up, and the fertilizer application and amount for each plot are shown in Table 22.

[0281] Table 22

[0282] project crop Fertilization treatment Fertilizer application rate Community 1 corn Process 1 80kg / mu Community 2 corn Process 2 80kg / mu Community 3 corn Process 1 80kg / mu Community 4 corn Process 2 80kg / mu Community 5 corn Process 3 80kg / mu Community 6 corn Process 4 80kg / mu Community 7 corn Process 3 80kg / mu Community 8 corn Process 4 80kg / mu

[0283] 5.4 Test Results

[0284] 5.4.1 Impact on Soil

[0285] Data collection time: April 10 and September 25, before fertilization.

[0286] Collection method: Before fertilization and before harvest, three sampling points were randomly selected from each plot to collect soil samples in the 0-20cm and 20-40cm depth ranges for soil nutrient testing. The test results are shown in Table 23.

[0287] Table 23

[0288]

[0289] The experimental results showed that, compared with the soil data after the application of fertilizer in each treatment, organic fertilizer granules K1, K2, and K3 could further improve soil structure, enhance soil properties, and reduce soil salinity in the topsoil compared with organic fertilizer granules DK1.

[0290] 5.4.2 Effects of different treatments on maize growth and yield

[0291] The differences in field growth among the treatments in the eight plots were observed, and the results are shown in Table 24.

[0292] Table 24

[0293] deal with Plant height (cm) Ear position (cm) Moisture content (wt%) Weight of 100 grains (g) Process 1 231.4 116.5 34.2 35.8 Process 2 227.1 117.6 35.2 37.7 Process 3 226.5 116.3 34.7 37.8 Process 4 228.8 118.6 34.2 38.2

[0294] After the corn matured, the yield was determined by the "harvesting method". The area of ​​corn harvested was 6 square meters (10m*0.6m). Two representative samples were taken from each treatment in the sub-district. The corn was threshed and dried indoors (the moisture content of the corn was below 14wt%) and weighed. The yield per mu was calculated. The results are shown in Table 25.

[0295] Table 25

[0296]

[0297] As can be seen from the table above, the treatments did not have a significant impact on corn growth, but the 100-grain weight of organic fertilizer granules K1, K2, and K3 was significantly higher than that of organic fertilizer granule DK1, which could further increase corn yield.

[0298] Analysis of variance (also known as one-way ANOVA) was used to study the variability in yield, and the results are shown in Table 26:

[0299] Table 26

[0300]

[0301] As shown in the table above, all treatments showed significant differences in yield (p<0.05), indicating that there were differences in yield among the different treatments. Specifically, the treatments showed significance at the 0.01 level (F=18.866, p=0.000), and the comparison of the mean scores of the groups with the most significant differences was "K1>DK1; K2>DK; K3>DK1", indicating that all treatments showed significant differences in yield.

[0302] The experimental results showed that when the same field fertilizer effect comparison test was conducted on organic fertilizer granules K1, K2, K3 and DK1, the results showed that organic fertilizer granules K1, K2 and K3 significantly increased the 100-grain weight and significantly increased the yield of corn compared with organic fertilizer granules DK1.

[0303] 6 trials 6

[0304] 6.1 Basic Information of the Test Site

[0305] 6.1.1 Test Location: Tiemaogedan Village, Taohai Town, Wuyuan County, Inner Mongolia

[0306] 6.1.2 Landform type: Alluvial plain

[0307] 6.1.3 Soil type and texture: Chestnut soil; medium to heavy soil.

[0308] 6.1.4 Test conditions: Open air

[0309] 6.1.5 Implementing Unit: Wuyuan County Wofeng Biotechnology Co., Ltd.

[0310] 6.1.6 Test period: May 15, 2023 – September 30, 2023

[0311] 6.2 Test Materials

[0312] Test crop: Sunflower (Helianthus annuus)

[0313] 6.3 Experimental Design

[0314] 6.3.1 Experimental Treatments: Fertilization was carried out on May 15, 2023, with four treatments: Treatment 1 (organic fertilizer granules DK1), Treatment 2 (organic fertilizer granules K1), Treatment 3 (organic fertilizer granules K2), and Treatment 4 (organic fertilizer granules K3) (Treatment 1 served as a control). These were applied to eight plots. The application rate was 80 kg / mu (approximately 13.33 kg / acre), and the application method was basal application. Except for the treatments described, the types and quantities of other basal fertilizers (chemical fertilizers) and topdressings were consistent.

[0315] 6.3.2 Field Design: The field area is 10.6m long and 63m wide, with a net area of ​​668m². 2 (equivalent to 1 mu)

[0316] Repeat twice, setting up protected rows. A total of 8 plots were set up, and the fertilizer application and amount for each plot are shown in Table 27.

[0317] Table 27

[0318] project crop Fertilization treatment Fertilizer application rate Community 1 sunflower Process 1 80kg / mu Community 2 sunflower Process 2 80kg / mu Community 3 sunflower Process 1 80kg / mu Community 4 sunflower Process 2 80kg / mu Community 5 sunflower Process 3 80kg / mu Community 6 sunflower Process 4 80kg / mu Community 7 sunflower Process 3 80kg / mu Community 8 sunflower Process 4 80kg / mu

[0319] 6.4 Test Results

[0320] 6.4.1 Impact on Soil

[0321] Data collection time: April 10 and September 25, before fertilization.

[0322] Collection method: Before fertilization and before harvest, three sampling points were randomly selected from each plot to collect soil samples in the 0-20cm and 20-40cm depth ranges, and the soil nutrients and bulk density were tested. The test results are shown in Tables 28 and 29.

[0323] Table 28

[0324]

[0325] Table 29

[0326]

[0327] The experimental results showed that, in the same field soil improvement and fertilizer effect comparison test of organic fertilizers K1, K2, K3 and DK1, organic fertilizer granules K1, K2 and K3 were able to further improve soil structure, enhance soil properties and reduce soil salinity in the topsoil compared to organic fertilizer DK1.

[0328] 6.4.2 Effects of different treatments on sunflower growth and yield

[0329] Before the sunflowers matured and were harvested, the yield was determined by the "cutting method". The sunflower cutting area was square meters (10m*0.6m). Three representative sampling points were taken for each treatment. The harvested seeds were weighed after being removed from the trays. The total number of plants in the plot was recorded and the yield per mu was calculated. The results are shown in Table 30.

[0330] Table 30

[0331]

[0332] The experimental results showed that, in a field comparison experiment of organic fertilizer granules K1, K2, K3 and DK1, the organic fertilizer granules K1, K2 and K3 significantly increased the weight of 100 sunflower seeds and the weight of seeds per disc compared with organic fertilizer granule DK1, and the yield was significantly increased.

[0333] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.

Claims

1. An organic fertilizer, characterized in that, On a dry basis, the organic fertilizer contains 0.9-2.5 wt% Ca, 3.1-6.9 wt% Cl, 0.05-0.15 wt% Mg, and 0.25-0.75 wt% small molecule animal protein.

2. The organic fertilizer according to claim 1, characterized in that, The molecular weight of the small molecule animal protein is ≤10000 Da, and more preferably 180-1000 Da.

3. The organic fertilizer according to claim 1 or 2, characterized in that, The weight ratio of Ca to small molecule animal protein in the organic fertilizer is 3.5-3.7:1; Preferably, the weight ratio of Ca, Cl, Mg and small molecule animal protein in the organic fertilizer is 3.5-3.7:9-12:0.16-0.2:

1.

4. A method for preparing an organic fertilizer, characterized in that, Includes the following steps: The waste liquid from bone gelatin production is concentrated to obtain a concentrate, which is then mixed with raw materials for organic fertilizer preparation; the raw materials for organic fertilizer preparation are livestock manure and / or animal and plant residues.

5. The preparation method according to claim 4, characterized in that, The solid content in the waste liquid from bone gelatin production is 5-7 wt%. Preferably, the pH of the bone gelatin production waste liquid is 6.5-7; Preferably, the Ca content in the bone gelatin production waste liquid is 1.4-2.1 wt%. Preferably, the Cl content in the bone gelatin production waste liquid is 2.9-4.1 wt%. Preferably, the Mg content in the bone gelatin production waste liquid is 0.04-0.07 wt%. Preferably, the content of small molecule animal protein in the bone gelatin production waste liquid is 0.4-0.6 wt%. Preferably, the molecular weight of the small-molecule animal protein in the bone gelatin production waste liquid is ≤10000Da, and more preferably 180-1000Da.

6. The preparation method according to claim 5, characterized in that, The solid content in the concentrate is 30-42 wt%. Preferably, the pH of the concentrate is 6.5-7; Preferably, the Ca content in the concentrate is 8-13 wt%. Preferably, the concentration of Cl in the concentrate is 17-25 wt%. Preferably, the Mg content in the concentrate is 0.27-0.38 wt%. Preferably, the concentration of small molecule animal protein in the concentrate is 2.5-3.6 wt%.

7. The preparation method according to any one of claims 4 to 6, characterized in that, The livestock manure is fermented and decomposed material, preferably fermented and decomposed sheep manure; the animal and plant remains are fermented and decomposed material. Preferably, the weight ratio of the concentrate to the raw materials for preparing organic fertilizer is 1:3-5.

8. The preparation method according to claim 7, characterized in that, Before mixing the concentrate with the organic fertilizer preparation raw materials, granulation auxiliary materials are added to the concentrate; Preferably, the granulation excipient is selected from at least one of water, amino acid aqueous solution, humic acid aqueous solution and microbial functional bacteria solution; Preferably, the weight ratio of the granulation excipient to the concentrate is 1:3-5.

9. The preparation method according to any one of claims 4 to 6, characterized in that, The method further includes: drying the mixed material to obtain the organic fertilizer; Preferably, the drying conditions include at least the following: the moisture content of the organic fertilizer is 20-30 wt%.

10. The application of the organic fertilizer according to any one of claims 1 to 3 and / or the organic fertilizer obtained by the preparation method according to any one of claims 4 to 9 in agricultural production.

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