Portable device for screening activity of fungal metabolites

Abstract

The application discloses a portable fungus metabolite activity screening integrated device, and belongs to the field of fungus metabolite activity screening, which comprises a supporting base, a sample extraction part arranged on the supporting base to place fungus dry powder, an extraction and purification part arranged on the supporting base and connected with the sample extraction part to take out the sample in the sample extraction part for purification, a suction head replacement part arranged on the supporting side plate to place the connecting suction head, so that the connecting suction head is new every time when the solution in the extraction and purification part is transferred, and a detection part arranged in the constant-temperature incubation cabin to detect the reacted solution. The synchronous rotation design of the sample disc and the purification disc, the precise pipetting of the mechanical arm and the automatic replacement of the suction head realize continuous pipeline operation, and manual operation of frequently transferring the sample and the reagent between different equipment in the traditional method is avoided.

Description

Technical Field

[0001] This invention relates to the field of fungal metabolite activity screening, and more specifically, to a portable integrated device for fungal metabolite activity screening. Background Technology

[0002] Fungi, especially marine fungi, are an important resource for discovering novel bioactive substances (such as antibacterial and antitumor compounds). Rapid and efficient activity screening of their metabolites is a key step in the discovery of microbial drugs and the control of aquatic diseases.

[0003] Currently, the activity screening of fungal metabolites mainly relies on traditional manual laboratory procedures, typically including the following steps: Sample pretreatment: The fungal hyphae or fermentation products are dried and ground, then soaked in organic solvents or extracted by ultrasound, followed by centrifugation, filtration, and other steps to obtain a crude extract. Sample dilution and loading: The crude extract is serially diluted, and the experimenter uses a manual pipette to add sample solutions of different concentrations one by one to the wells of a multi-well plate. Inoculation and incubation: The target bacterial suspension is added to each well and incubated in a large constant temperature incubator for a certain period of time. Detection and analysis: After incubation, the multi-well plate is transferred to an optical detection device such as an ELISA reader to measure the absorbance or fluorescence value of each well, and finally, the activity indicators such as the inhibition rate are calculated manually.

[0004] Existing technologies involve multiple sample transfers between different containers and devices, requiring numerous manual steps. This increases the risk of cross-contamination or sample loss, affecting the accuracy of results. The heavy reliance on manual pipetting is inefficient and hinders the parallel processing of large batches of samples. Furthermore, unavoidable errors in manual operation lead to poor experimental repeatability and reduced data reliability. Summary of the Invention

[0005] To solve the above problems, the present invention adopts the following technical solution.

[0006] A portable integrated device for screening fungal metabolite activity includes: a support base; and further includes: A sample extraction unit is disposed on the support base to hold dried fungal powder; An extraction and purification unit is disposed on the support base and is connected to the sample extraction unit to remove the sample from the sample extraction unit for purification. A constant temperature incubation chamber is mounted on the support base and is located on one side of the extraction and purification section; The reaction section is located inside the constant temperature incubation chamber, allowing the fungi to undergo a suitable chemical reaction. A supporting side plate is provided on the supporting base; A micro-volume sampling robotic arm is mounted on the support side plate to transfer the solution in the extraction and purification section to the reaction section. The micro-volume sampling robotic arm is equipped with a connecting pipette tip. A pipette tip replacement section is provided on the support side plate to hold the connecting pipette tip, so that each time the solution in the extraction and purification section is transferred, the connecting pipette tip is brand new; The detection unit is located inside the constant temperature incubation chamber to detect the solution after the reaction; A control display unit is mounted on the support base to control the movement trajectory of the micro-sampling robotic arm and the detection results obtained by the detection unit.

[0007] Furthermore, the support base includes: The top plate is supported and connected to the constant temperature incubation chamber; A supporting base plate is disposed at the bottom end of the supporting top plate; A support block is connected to the support base plate to stabilize the support device.

[0008] Furthermore, the sample extraction unit includes: The drive motor is mounted on the support base plate; The first rotating shaft has one end connected to the output end of the drive motor; The drive gear is sleeved on the first rotating shaft and located at the bottom end of the supporting top plate; The sample tray is connected at one end to the other end of the first rotating shaft and is located at the top of the supporting top plate; The sample tray has several sets of placement cavities arranged in a circumferential array to hold fungal powder. Rotate the connecting cylinder to make it rotatably connected to the bottom of the sample tray; The first connecting shaft has one end connected to the rotating connecting cylinder; A sealing base plate is connected to the other end of the first connecting shaft and is located at the bottom of the corresponding placement cavity to prevent fungal powder from leaking out. A torsion spring is disposed inside the rotating connecting cylinder, connected to the bottom end of the sample tray, and fixedly connected to the rotating connecting cylinder to drive the rotating connecting cylinder to rotate.

[0009] Furthermore, the extraction and purification unit includes: The purification tray is located on the upper part of the support top plate and is attached to one side of the bottom of the sample tray; The second rotating shaft has one end connected to the bottom of the purification tray and the other end passing through the supporting top plate; The driven gear is connected to the other end of the second rotating shaft and meshes with the driving gear; The purification cavities are arranged in several groups, and the several groups of purification cavities are arranged in a circumferential array on the sample tray to place fungal dry powder and purification solution. The number of purification cavities corresponds to the number of placement cavities. Limiting grooves are formed on both sides of the purification cavity; A miniature extraction tube is placed inside the purification cavity to hold the purification solution; Limiting blocks are disposed on both sides of the micro extraction tube and are connected to the limiting groove to prevent the micro extraction tube from falling out of the purification cavity; An ultrasonic device is installed at the center of the top of the purification tray to perform ultrasonic extraction on the solution in the micro extraction tube.

[0010] Furthermore, the constant temperature incubation chamber includes: The cabin is mounted on the supporting top plate; The hatch has one end rotatably connected to the cabin body to form an openable / closing design; The first heat-dissipating plate is disposed inside the cabin to provide a heat source; The second heat-dissipating plate is located at the top inner side of the hatch, and works in conjunction with the first heat-dissipating plate to provide a heat source from all directions.

[0011] Furthermore, the reaction section includes: The microfluidic reaction chip module is placed inside the chamber; The reaction chamber is provided in several groups, and these groups of reaction chambers are used to place the target solution for reaction.

[0012] Furthermore, the suction head replacement unit includes: The replacement box is located on the support side plate and above the purification tray; Replacement holes are provided at the top of the replacement box, and there are several sets of them, corresponding to the number of purification holes. The replacement drawer slides within the replacement box, facilitating easy placement and removal; A replacement slot is provided on the replacement drawer and is correspondingly provided with the replacement hole to place the connecting suction head.

[0013] Furthermore, the detection unit includes: A solution turbidity detection block is installed at the top of the inner wall of the chamber cover to detect the turbidity properties of the solution after the reaction in the reaction chamber. A fluorescence intensity detection block is installed at the top of the inner wall of the chamber cover to detect the fluorescence intensity properties of the solution after the reaction in the reaction chamber.

[0014] Furthermore, the control display unit includes: The main control board is mounted on the supporting base plate to realize motor control, temperature control and signal acquisition; A touch screen is mounted on the top support plate to display the operation interface and test results.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention integrates multiple independent steps required for fungal metabolite activity screening, including sample transfer, extraction and purification, micro-volume addition, isothermal reaction, and optical detection, into a single portable device for automated completion. Through the synchronized rotation of the sample and purification trays, precise liquid transfer by the robotic arm, and automatic tip changing, continuous pipeline operation is achieved. This avoids the manual operation of frequently transferring samples and reagents between different devices in traditional methods, significantly shortening detection time and enabling parallel processing of multiple samples, thus significantly improving screening throughput. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0017] Figure 1 This is a first perspective view of the present invention; Figure 2 This is a second perspective view of the present invention; Figure 3 This is a first cross-sectional perspective view of the present invention; Figure 4 This is a first partial perspective view of the present invention; Figure 5 This is a first partially exploded perspective view of the present invention; Figure 6 This is a partial perspective view of the sample extraction section of the present invention; Figure 7 for Figure 6 Enlarged view of point A; Figure 8 This is a second partial perspective view of the present invention; Figure 9 for Figure 8 Enlarged view of point B; Figure 10 This is a second partial exploded perspective view of the present invention; Figure 11 This is a third partially exploded perspective view of the present invention; Figure 12 This is a third partial perspective view of the present invention.

[0018] Explanation of the labels in the diagram: 1. Support base; 101. Support side plate; 102. Support base block; 103. Support base plate; 104. Support top plate; 2. Sample extraction section; 201. Drive motor; 202. Drive gear; 204. First rotating shaft; 205. Sample tray; 206. Placement cavity; 207. Sealing base plate; 208. First connecting shaft; 209. Rotating connecting cylinder; 210. Torsion spring; 3. Control and display section; 301. Main control board; 302. Touch screen display; 4. Extraction and purification section; 401. Second rotating shaft; 402. Purification tray; 403. Driven gear; 404. Ultrasonic equipment; 405. Purification hole 406. Miniature extraction tube; 407. Limiting block; 408. Limiting groove; 5. Constant temperature incubation chamber; 501. Chamber body; 502. Chamber cover; 503. First heat release plate; 504. Second heat release plate; 6. Reaction section; 601. Microfluidic reaction chip module; 602. Reaction chamber; 7. Micro-volume sampling robotic arm; 701. Miniature pipette; 702. Connecting pipette tip; 703. Adjustment sensor; 8. Pipette tip replacement section; 801. Replacement box; 802. Replacement hole; 803. Replacement drawer; 804. Replacement groove; 805. Pull block; 901. Solution turbidity detection block; 902. Fluorescence intensity detection block. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0020] like Figures 1 to 12As shown, a portable integrated device for screening the activity of fungal metabolites includes: a support base 1; a sample extraction unit 2 disposed on the support base 1 for holding fungal dry powder; an extraction and purification unit 4 disposed on the support base 1 and connected to the sample extraction unit 2 for removing and purifying the sample from the sample extraction unit 2; a constant temperature incubation chamber 5 disposed on the support base 1 and located to one side of the extraction and purification unit 4; a reaction unit 6 disposed within the constant temperature incubation chamber 5 to allow the fungi to undergo a suitable chemical reaction; a support side plate 101 disposed on the support base 1; and a micro-volume sampling robotic arm 7. The micro-volume loading robotic arm 7 is mounted on the support side plate 101 to transfer the solution in the extraction and purification section 4 to the reaction section 6. A connecting pipette tip 702 is provided on the micro-volume loading robotic arm 7. A pipette tip replacement section 8, also mounted on the support side plate 101, is used to hold the connecting pipette tip 702, ensuring that a brand new connecting pipette tip 702 is used each time the solution in the extraction and purification section 4 is transferred. A detection section, located inside the constant temperature incubation chamber 5, is used to detect the solution after the reaction. A control and display section 3, mounted on the support base 1, controls the movement trajectory of the micro-volume loading robotic arm 7 and the detection results obtained by the detection section.

[0021] In this embodiment of the invention, a sample extraction unit 2 is provided, on which the sample to be purified and tested is placed; an extraction and purification unit 4 is provided to purify the sample one by one; and a micro-volume sampling robotic arm 7 is provided to transfer the purified solution to the reaction unit 6 for reaction, thereby drawing conclusions through detection by the detection unit. Thus, various required properties of fungal metabolites can be obtained quickly on one device.

[0022] like Figures 1 to 4 As shown, the support base 1 includes: a support top plate 104, which is connected to the constant temperature incubation chamber 5; a support bottom plate 103, which is disposed at the bottom end of the support top plate 104; and a support bottom block 102, which is connected to the support bottom plate 103 to stabilize the support device.

[0023] In this embodiment of the invention, a top support plate 104 and a bottom support plate 103 are provided so that modules that do not need to be exposed can be placed in the middle; a bottom support block 102 is provided to ensure the overall stability of the equipment.

[0024] like Figures 5 to 7As shown, the sample extraction unit 2 includes: a drive motor 201, mounted on the support base plate 103; a first rotating shaft 204, one end of which is connected to the output end of the drive motor 201; a drive gear 202, sleeved on the first rotating shaft 204 and located at the bottom end of the support top plate 104; a sample disk 205, one end of which is connected to the other end of the first rotating shaft 204 and located at the top end of the support top plate 104; and placement cavities 206, of which several groups are arranged, and the several groups of placement cavities 206 are circumferentially arrayed on the sample. The sample tray 205 is used to hold dried fungal powder; a rotating connecting cylinder 209 is rotatably connected to the bottom of the sample tray 205; a first connecting shaft 208 is connected at one end to the rotating connecting cylinder 209; a sealing bottom plate 207 is connected to the other end of the first connecting shaft 208 and is located at the bottom of the corresponding placement cavity 206 to prevent the dried fungal powder from leaking out; a torsion spring 210 is disposed inside the rotating connecting cylinder 209, connected to the bottom of the sample tray 205, and fixedly connected to the rotating connecting cylinder 209 to drive the rotating connecting cylinder 209 to rotate.

[0025] like Figures 5 to 9 As shown, the extraction and purification unit 4 includes: a purification disk 402, disposed on the upper end of the support top plate 104 and fitted to one side of the bottom end of the sample disk 205; a second rotating shaft 401, one end of which is connected to the bottom end of the purification disk 402, and the other end of which passes through the support top plate 104; a driven gear 403, connected to the other end of the second rotating shaft 401 and meshing with the driving gear 202; and purification cavities 405, which are arranged in a plurality of groups, the plurality of groups of purification cavities 405 being arranged in a circumferential array on the sample disk 205 to hold fungal dry powder and purification solution. The number of purification cavities 405 corresponds to the number of placement cavities 206; limiting grooves 408 are formed on both sides of the purification cavities 405; a micro extraction tube 406 is placed inside the purification cavity 405 to hold the purification solution; limiting blocks 407 are set on both sides of the micro extraction tube 406 and are connected to the limiting grooves 408 to prevent the micro extraction tube 406 from falling out of the purification cavity 405; an ultrasonic device 404 is installed at the center of the top of the purification plate 402 to perform ultrasonic extraction of the solution in the micro extraction tube 406.

[0026] In this embodiment of the invention, after manually placing a quantitative amount of fungal metabolites one by one into the placement cavity 206, the control display unit 3 controls the drive motor 201 to rotate, which in turn drives the first rotating shaft 204 to rotate, thereby driving the sample tray 205 to rotate. Simultaneously, the first rotating shaft 204 drives the drive gear 202 to rotate, which in turn drives the driven gear 403 to rotate, thereby driving the second rotating shaft 401 to rotate. The second rotating shaft 401 drives the purification tray 402 to rotate. Since the bottom of the purification tray 402 partially overlaps with the bottom of the sample tray 205, during the rotation of the purification tray 402, it will encounter the sealing base plate 207 at the bottom of the sample tray 205, thus preventing the sealing base plate 207 from overlapping with the placement cavity 206. After the purification tray 402 and the sample tray 205 have rotated a certain angle, the corresponding placement cavity 206 and the purification tray 205... When the cavities 405 overlap, the purification disc 402 drives the sealing base plate 207 to rotate along the rotating connecting cylinder 209 at a certain angle, causing the fungal metabolites in the placement cavity 206 to fall directly into the micro-extraction cylinder 406 in the purification cavity 405. As rotation continues, the corresponding placement cavity 206 and purification cavity 405 move away from each other, and the corresponding sealing base plate 207 is released from the restriction of the purification disc 402. Under the action of the torsion spring 210, it rotates in the opposite direction back to the bottom of the placement cavity 206, facilitating the next placement. Simultaneously, rotation continues, ensuring that each corresponding placement cavity 206 and purification cavity 405 completes contact, transferring the fungal metabolites in the placement cavity 206 to the micro-extraction cylinder 406 in the purification cavity 405. After one rotation of the sample disc 205 and purification disc 402, the transfer of all fungal metabolites is completed.

[0027] After transferring the fungal metabolites into the micro-extraction tube 406, which is pre-filled with extraction solution and adsorbent material, the ultrasonic device 404 is activated, immediately generating ultrasound waves to extract the mixture within the micro-extraction tube 406. The energy of the ultrasound waves efficiently dissolves the target metabolites in the fungal powder into the solvent, forming a crude extract. Simultaneously, the adsorbent material selectively adsorbs impurities, pigments, lipids, or irrelevant compounds. Finally, after standing for a period of time, a clear suspension is obtained, thus completing the purification of the fungal metabolites.

[0028] By using a sample tray 205 and a purification tray 402 in a coordinated connection, samples can be loaded all at once. This avoids the need for manual labor, where samples are added one by one to the purification tray 402 after each test. Furthermore, because adjacent purification cavities 405 on the purification tray 402 are very close together, manual handling of dried fungal powder easily leads to dust splashing or spillage. Performing this operation above a purification tray with multiple open solvent holes can easily cause cross-contamination between samples, resulting in inaccurate results. The coordinated mechanism of the sample tray 205 and purification tray 402 reduces tedious manual operations; samples can be loaded into the sample tray 205 all at once, while also avoiding cross-contamination caused by human error.

[0029] like Figure 10 As shown, the constant temperature incubation chamber 5 includes: a chamber body 501, which is disposed on the supporting top plate 104; a chamber cover 502, one end of which is rotatably connected to the chamber body 501 to form an openable and closable design; a first heat-dissipating plate 503, which is disposed on the inner side of the chamber body 501 to provide a heat source; and a second heat-dissipating plate 504, which is disposed on the top inner side of the chamber cover 502 to provide an all-around heat source in conjunction with the first heat-dissipating plate 503.

[0030] like Figure 10 As shown, the reaction unit 6 includes: a microfluidic reaction chip module 601, placed inside the chamber 501; and a reaction chamber 602, which has several sets, and the several sets of reaction chambers 602 are used to place the target solution for reaction.

[0031] In this embodiment of the invention, after purification, a quantitative amount of purified fungal metabolite solution is automatically transferred to the reaction chamber 602 by a robotic arm. The robotic arm employs a three-axis sliding stage structure, is equipped with a micropipette 701, and features a tip changing unit 8, enabling automatic sampling, sample addition, and tip replacement. The movement of the robotic arm is controlled by a control and display unit, allowing it to precisely transfer the solution from the purification tray 402 to the microfluidic reaction chip module 601. After replacing the connecting tip 702 at the tip changing unit 8, the solution transfer continues. The micropipette 701 is a laboratory instrument used for accurately measuring and transferring minute amounts of liquid, ensuring high precision in liquid transfer.

[0032] The microfluidic reaction chip module 601 is equipped with multiple parallel reaction chambers 602. Each reaction chamber 602 is pre-injected with target bacterial suspension and indicator dye. After sample injection, the reaction is carried out under constant temperature conditions to perform the specified reaction and obtain the desired property judgment.

[0033] After the reaction solution is placed in the reaction chamber 602, the chamber cover 502 is manually closed, and the first heat-releasing plate 503 and the second heat-releasing plate 504 generate a certain amount of heat to provide a suitable constant temperature in the constant temperature incubation chamber 5, so as to facilitate the reaction in the reaction chamber 602.

[0034] like Figure 11 As shown, the suction head replacement part 8 includes: a replacement box 801, which is disposed on the support side plate 101 and located at the upper end of the purification tray 402; a replacement hole 802, which is opened at the top of the replacement box 801 and is provided in several sets, corresponding to the number of purification hole cavities 405; a replacement drawer 803, which slides inside the replacement box 801 and is convenient for placement and removal; and a replacement slot 804, which is opened on the replacement drawer 803 and is provided corresponding to the replacement hole 802 for placing the connecting suction head 702.

[0035] In this embodiment of the invention, when transferring the purified fungal metabolite solution, the drive motor 201 is continuously activated so that all the target micro-extraction cylinders 406 are in the same position. That is, after transferring the solution from one micro-extraction cylinder 406 to the reaction chamber 602, it is rotated by a certain angle so that the next micro-extraction cylinder 406 to be transferred is in the same position, which facilitates the path planning of the robotic arm. When transferring the solution, the robotic arm drives the connecting pipette tip 702 to move to the designated micro-extraction cylinder 406. After the micropipette 701 controls the adsorption of a certain amount of solution, the robotic arm moves to the designated... After moving above reaction chamber 602, the solution is transferred into reaction chamber 602. The robotic arm then moves above replacement box 801 and places the used connecting pipette tip 702 into replacement slot 804. A new connecting pipette tip 702 is then connected from another replacement slot 804, thus completing the replacement of the connecting pipette tip 702. The system starts vacuum adsorption, and at the same time, the connector and connecting pipette tip 702 are physically locked together through interference fit. An adjustment sensor 703 is set to confirm that the pipette tip is securely installed. The robotic arm is lifted, carrying the new pipette tip away from replacement box 801, ready to move to the next position to perform the transfer task, and so on.

[0036] Since the number of replacement tanks 804 is equal to the number of reaction chambers 602, after all the solutions have been transferred, the connecting pipette tips 702 in the replacement tanks 804 are replaced and become old connecting pipette tips. Then, the pull block 805 can be manually pulled to pull the replacement drawer 803 out of the replacement box 801 to update the connecting pipette tips 702.

[0037] like Figures 1 to 10As shown, the detection unit includes: a solution turbidity detection block 901, which is disposed at the top of the inner wall of the cover 502 to detect the turbidity properties of the solution after reaction in the reaction chamber 602; and a fluorescence intensity detection block 902, which is disposed at the top of the inner wall of the cover 502 to detect the fluorescence intensity properties of the solution after reaction in the reaction chamber 602.

[0038] In this embodiment of the invention, after the reaction is completed, both the solution turbidity detection block 901 and the fluorescence intensity detection block 902 can directly obtain the detection results, which are then transmitted to the control and display unit for analysis and judgment.

[0039] like Figures 1 to 4 As shown, the control and display unit 3 includes: a main control board 301, which is disposed on the supporting base plate 103 to realize motor control, temperature control and signal acquisition; and a touch screen 302, which is disposed on the supporting top plate 104 to display the operation interface and detection results.

[0040] In this embodiment of the invention, a main control board 301 is provided to control the start of the drive motor 201, the movement trajectory of the robotic arm and the temperature control of the constant temperature incubation chamber 5. At the same time, the detection results of the solution turbidity detection block 901 and the fluorescence intensity detection block 902 are analyzed and reflected on the touch screen 302.

[0041] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and its improved concept, should be covered within the scope of protection of the present invention.

Claims

1. A portable integrated device for screening the activity of fungal metabolites, comprising: Support base (1); characterized in that it further includes: The sample extraction unit (2) is set on the support base (1) to hold the dried fungal powder; The extraction and purification unit (4) is set on the support base (1) and is connected to the sample extraction unit (2) to remove the sample from the sample extraction unit (2) for purification. The constant temperature incubation chamber (5) is set on the support base (1) and is located on one side of the extraction and purification section (4); The reaction section (6) is located inside the constant temperature incubation chamber (5) to allow the fungi to undergo a suitable chemical reaction; A support side plate (101) is disposed on the support base (1); A micro-volume loading robotic arm (7) is mounted on the support side plate (101) to transfer the solution in the extraction and purification section (4) to the reaction section (6). A connecting pipette tip (702) is provided on the micro-volume loading robotic arm (7). A pipette tip replacement unit (8) is provided on the support side plate (101) to place the connecting pipette tip (702) so that each time the solution in the extraction and purification unit (4) is transferred, the connecting pipette tip (702) is brand new. The detection unit is located inside the constant temperature incubation chamber (5) to detect the solution after the reaction; A control display unit (3) is provided on the support base (1) to control the movement trajectory of the micro-sampling robotic arm (7) and the detection results obtained by the detection unit; The support base (1) includes: The top plate (104) is connected to the constant temperature incubation chamber (5); A supporting base plate (103) is disposed at the bottom end of the supporting top plate (104); A support block (102) is connected to the support base plate (103) to stabilize the support device; The sample extraction unit (2) includes: A drive motor (201) is mounted on the support base plate (103); The first rotating shaft (204) is connected at one end to the output end of the drive motor (201); The drive gear (202) is sleeved on the first rotating shaft (204) and is located at the bottom end of the support top plate (104); The sample plate (205) is connected at one end to the other end of the first rotating shaft (204) and is located at the top of the support plate (104); The placement cavity (206) is provided in several groups, and the several groups of placement cavities (206) are arranged in a circumferential array on the sample plate (205) to place fungal dry powder; Rotate the connecting cylinder (209) to rotatably connect it to the bottom end of the sample tray (205); The first connecting shaft (208) has one end connected to the rotating connecting cylinder (209); A sealing base plate (207) is connected to the other end of the first connecting shaft (208) and is located at the bottom of the corresponding placement cavity (206) to prevent fungal powder from leaking out; A torsion spring (210) is disposed inside the rotating connecting cylinder (209), connected to the bottom end of the sample tray (205), and fixedly connected to the rotating connecting cylinder (209) to drive the rotating connecting cylinder (209) to rotate.

2. The portable integrated device for screening fungal metabolite activity according to claim 1, characterized in that, The extraction and purification unit (4) includes: The purification tray (402) is disposed on the upper end of the support top plate (104) and is attached to one side of the bottom end of the sample tray (205); The second rotating shaft (401) is connected at one end to the bottom end of the purification plate (402), and at the other end passes through the support top plate (104). The driven gear (403) is connected to the other end of the second rotating shaft (401) and meshes with the driving gear (202); The purification pores (405) are provided in several groups, and the several groups of purification pores (405) are arranged in a circumferential array on the sample plate (205) to place fungal dry powder and purification solution. The number of purification pores (405) is equal to the number of placement pores (206). A limiting groove (408) is provided on both sides of the purification cavity (405); A micro extraction tube (406) is placed inside the purification cavity (405) to hold the purification solution; Limiting blocks (407) are disposed on both sides of the micro extraction tube (406) and are connected to the limiting groove (408) to prevent the micro extraction tube (406) from falling out of the purification cavity (405); An ultrasonic device (404) is installed at the center of the top of the purification plate (402) to perform ultrasonic extraction on the solution in the micro extraction tube (406).

3. The portable integrated device for screening fungal metabolite activity according to claim 2, characterized in that, The constant temperature incubation chamber (5) includes: The cabin (501) is mounted on the supporting top plate (104); The hatch (502) is rotatably connected to the cabin body (501) at one end to form an opening and closing design; The first heat-dissipating plate (503) is disposed inside the cabin (501) to provide a heat source; The second heat-dissipating plate (504) is disposed on the top inner side of the hatch (502) and works with the first heat-dissipating plate (503) to provide an all-round heat source.

4. The portable integrated device for screening fungal metabolite activity according to claim 3, characterized in that, The reaction section (6) includes: A microfluidic reaction chip module (601) is placed inside the chamber (501); The reaction chamber (602) is provided with several sets, and the several sets of reaction chambers (602) are used to place the target solution for reaction.

5. The portable integrated device for screening fungal metabolite activity according to claim 4, characterized in that, The suction head replacement unit (8) includes: The replacement box (801) is disposed on the support side plate (101) and is located at the upper end of the purification plate (402); Replacement holes (802) are provided at the top of the replacement box (801), and there are several sets of them, corresponding to the number of purification holes (405); The replacement drawer (803) slides within the replacement box (801) and is easy to place and remove; A replacement slot (804) is provided on the replacement drawer (803) and is provided corresponding to the replacement hole (802) to place the connecting suction head (702).

6. The portable integrated device for screening fungal metabolite activity according to claim 5, characterized in that, The detection unit includes: A solution turbidity detection block (901) is installed at the top of the inner wall of the cover (502) to detect the turbidity properties of the solution after reaction in the reaction chamber (602); A fluorescence intensity detection block (902) is disposed at the top of the inner wall of the cover (502) to detect the fluorescence intensity properties of the solution after reaction in the reaction chamber (602).

7. The portable integrated device for screening fungal metabolite activity according to claim 6, characterized in that, The control display unit (3) includes: The main control board (301) is mounted on the supporting base plate (103) to realize motor control, temperature control and signal acquisition; A touch screen (302) is disposed on the supporting top plate (104) to display the operation interface and detection results.

Images