A temperature control and micro-precise sample adding linkage device for enzyme activity detection

Abstract

The present application relates to the technical fields of enzyme activity detection, and specifically relates to a temperature control and micro-adding precise linkage device for enzyme activity detection, which comprises a detection machine, a water bath box is detachably installed and fixed at the rear of the detection machine, a supporting ring is fixed in the water bath box, a supporting shaft is rotationally connected at the center position of the supporting ring in the water bath box, a plurality of sleeve rings are annularly and equiangularly fixed at the outer side of the supporting shaft, a cylinder one in contact with the supporting ring is movably sleeved on the sleeve ring, and the cylinder one is used for containing amylase and a substrate containing starch. In the present application, the maximum enzyme reaction limit is detected by gradient increasing of the substrate amount under the condition of fixed enzyme amount, so that the temperature control and micro-adding of the substrate can be carried out according to the needs for the enzyme activity detection in sequence by one device, and the enzyme activity detection and the maximum enzyme reaction limit detection are combined and implemented in linkage, which is helpful to improve the overall efficiency of the enzyme activity detection.

Description

Technical Field

[0001] This invention relates to the field of enzyme activity detection technology, specifically a precise linkage device for temperature control and micro-volume sample addition for enzyme activity detection. Background Technology

[0002] Enzyme-catalyzed reactions are significantly affected by temperature and substrate concentration. To comprehensively evaluate enzyme activity, two key experiments are often required: one is a temperature gradient experiment (to determine the optimal reaction temperature of the enzyme), and the other is a substrate amount increase gradient experiment (to determine the maximum reaction limit of the enzyme). Taking amylase as an example, it is necessary to measure amylase activity at different temperatures and to gradually increase the amount of starch-containing substrate at a fixed enzyme amount. In the current technology, temperature control and substrate micro-addition for enzyme activity detection are usually accomplished by two independent devices. This can easily lead to: fragmented experimental procedures, making it impossible to obtain multi-dimensional data simultaneously; the need for frequent manual changes of reaction vessels to hold substrate and enzyme, which is time-consuming and prone to introducing errors; and difficulty in linking temperature control and substrate micro-addition for enzyme activity detection to improve the overall efficiency of enzyme activity detection. Summary of the Invention

[0003] The purpose of this invention is to provide a precise linkage device for temperature control and micro-volume sample addition for enzyme activity detection, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, the present invention provides the following technical solution: A precise temperature control and micro-volume sampling linkage device for enzyme activity detection includes: Testing machine; A water bath tank, wherein a support ring is fixed inside the water bath tank; A support shaft is rotatably connected to a water bath. Multiple collars are evenly fixed on the outer side of the support shaft, and a cylinder that contacts the support ring is sleeved on the collar. An infusion assembly, comprising two cylindrical tubes mounted to a water bath, wherein a hollow cylinder is slidably connected inside the cylindrical tubes. A sample feeding assembly, comprising an annular cylinder rotatably sleeved with a support shaft, a gooseneck tube fixedly connected to the outer side of the annular cylinder, and a movable cover slidably connected to the opening of the annular cylinder; The replacement assembly includes a sleeve fixed to the testing machine, and two electric push rods are arranged on the outer side of the sleeve; An L-shaped plate is fixed to a water bath, and a bent pipe is fixed to the L-shaped plate.

[0005] Furthermore, the top of the support ring is fixed with a protrusion that abuts against the bottom surface of the cylinder, and an inclined tube is fixed to the outside of the sleeve.

[0006] Furthermore, a collection box is fixed to the outside of the water bath, and a heating block is fixed to the bottom of the water bath.

[0007] The infusion assembly also includes an L-shaped frame 1 fixed to the water bath, a screw 2 rotatably connected to the L-shaped frame 1, and a crossbeam fixed to the hollow cylinder screwed to the outside of the screw 2.

[0008] Furthermore, a connecting plate is fixed between the sleeve and the testing machine, multiple cylinders are placed inside the sleeve, a motor is fixed at the bottom of the sleeve, and an eccentric wheel is fixed at the output end of the motor.

[0009] Furthermore, a camera is installed on the L-shaped plate, and the bent pipe is connected and fixed to the pipe that transports iodine solution.

[0010] Furthermore, the bottom of the testing machine is fixed with a second motor that can drive the support shaft to rotate, and the top of the support shaft is fixed with a first screw.

[0011] Furthermore, the top of the movable cover is fixed with an L-shaped block that is screwed into the screw, and an L-shaped frame is fixed between the annular cylinder and the water bath. A positioning rod that is slidably connected to the L-shaped block is fixed on the L-shaped frame.

[0012] Furthermore, the two electric actuators are fixedly connected to the annular cylinder and the second sleeve, respectively, and the output ends of the two electric actuators are fixed with the first arc plate and the third arc plate, respectively.

[0013] Furthermore, a corrugated suction cup and a second curved plate are fixed on the first curved plate, and the corresponding arcs of the second and third curved plates share the same center.

[0014] Compared with the prior art, the beneficial effects of the present invention are: Multiple cylinders (Cylinder 1) are installed inside a water bath. Two cylinders (Cylinder 2) are installed inside the water bath, one containing amylase solution and the other containing a starch-containing substrate solution. A motor drives a screw to rotate, causing a crossbeam to move the two hollow cylinders downwards along the two cylinders (Cylinder 2). This raises the liquid level inside the two cylinders (Cylinder 2), allowing the substrate and amylase to be simultaneously transported to cylinder 1 through an inclined tube on the outside of cylinder 2 for catalytic reaction. Multiple cylinders (Cylinder 1) can hold multiple mixtures of enzyme and substrate. The water temperature inside the water bath is gradually increased according to a gradient. After each temperature increase, the enzyme and substrate catalytic reaction is allowed to proceed for a period of time. Then, a suitable amount of iodine solution is added to cylinder 1 below the L-shaped plate via a curved tube. Observing whether the solution inside cylinder 1 turns blue assesses whether the enzyme has catalyzed the hydrolysis of starch. Multiple cylinders (Cylinder 1) can be added sequentially at different temperature gradients, thus enabling enzyme activity detection at multiple temperature gradients.

[0015] An annular cylinder containing a substrate solution is fitted onto a support shaft. A screw at the top of the support shaft, via an L-shaped block, gradually presses down the substrate solution inside the annular cylinder. The substrate solution is then injected into the second cylinder containing the substrate solution through a gooseneck tube. As the subsequent hollow cylinder moves down a certain distance, the amount of substrate solution added to the first cylinder gradually increases. Multiple cylinders can rotate to the bottom of the inclined tube to hold different amounts of substrate solution, following a gradient increase in substrate amount. A water bath is used to heat the multiple cylinders for a period of time. Then, iodine solution is added to all cylinders through a bent tube. The color of the solution inside the first cylinder is observed to assess whether the enzyme has catalyzed the hydrolysis of starch. This allows for the detection of the enzyme's maximum reaction limit by gradually increasing the substrate amount while maintaining a fixed enzyme amount. This enables a single device to perform temperature control and micro-substrate addition for enzyme activity detection as needed. The combined implementation of enzyme activity detection and maximum reaction limit detection helps improve the overall efficiency of enzyme activity detection.

[0016] By arranging support rings on the bottom surface of multiple cylinders, when the cylinder after the experiment rotates to the position of the protrusion on the support ring, the protrusion can lift the cylinder so that the bottom surface of the cylinder is flush with the top surface of the collar. The output ends of the two electric push rods extend so that the arc plate one and arc plate three are arranged outside the lifted cylinder. Then, the output end of one electric push rod extends so that the corrugated suction cup is attached to the outside of the used cylinder. The other electric push rod carries the arc plate three away from the cylinder. The electric push rod carries the covered cylinder through the corrugated suction cup to move above the collection box and release the cylinder. Thus, the used cylinder can automatically leave the device for collection. The motor drives the eccentric wheel to rotate, releasing a cylinder to fall onto the collar below the sleeve. The support shaft drives the collar to rotate between the arc plate three and the arc plate two towards the inclined tube. During the rotation, the bottom surface of the rear-placed cylinder gradually moves away from the protrusion on the support ring, allowing the cylinder to gradually move down so that the collar can stably fit on the outside of the cylinder. This achieves automatic loading of a new cylinder to hold the enzyme and substrate mixture, eliminating the need for manual replacement of the cylinder and helping to improve the efficiency of the entire enzyme activity detection. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the invention. Figure 1 ; Figure 2 This is a schematic diagram of the overall structure of the invention. Figure 2 ; Figure 3 This is a schematic diagram of the overall structure of the top of the water bath tank in this invention. Figure 1 ; Figure 4 This is a schematic diagram of the overall structure of the top of the water bath tank in this invention. Figure 2 ; Figure 5This is a schematic diagram of the water bath, L-shaped plate, cylinder II, and support ring structure in this invention; Figure 6 This is a schematic diagram of the replacement component structure in this invention. Figure 1 ; Figure 7 This is a schematic diagram of the replacement component structure in this invention. Figure 2 ; Figure 8 This is a schematic diagram of the sample addition component structure in this invention.

[0018] In the diagram: 100, Detector; 110, Motor 1; 200, Water bath; 210, Support ring; 211, Protrusion; 220, Collection box; 230, Heating block; 300, Support shaft; 310, Collar; 311, Cylinder 1; 320, Screw 1; 400, Infusion assembly; 410, Cylinder 2; 411, Inclined tube; 420, Hollow cylinder; 421, Crossbeam; 422, Notch; 430, L-shaped frame 1; 431, Screw 2; 5 00. Sample feeding assembly; 510. Annular cylinder; 520. Gooseneck tube; 530. Movable cover; 531. L-shaped block; 540. L-shaped frame II; 541. Positioning rod; 600. Replacement assembly; 610. Sleeve; 620. Electric push rod; 630. Arc plate I; 631. Corrugated suction cup; 632. Arc plate II; 640. Arc plate III; 650. Motor; 651. Eccentric wheel; 700. L-shaped plate; 710. Bend; 720. Camera. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Example 1, please refer to Figure 1 - Figure 8In this embodiment of the invention, a temperature control and micro-volume sampling precision linkage device for enzyme activity detection includes a detection machine 100. A water bath 200 is detachably installed and fixed at the rear of the detection machine 100. A support ring 210 is fixed inside the water bath 200. A support shaft 300 is rotatably connected inside the water bath 200 and located at the center of the support ring 210. Multiple collars 310 are fixed at equal angles in an annular shape on the outer side of the support shaft 300. A cylinder 311 is movably sleeved on the collar 310 and contacts the support ring 210. The cylinder 311 is used to hold amylase and a starch-containing substrate. An infusion assembly 400 is arranged at one end of the water bath 200 to add amylase and substrate to the cylinder 311. The infusion assembly 400 includes two cylinders 410 detachably installed with the water bath 200. The two cylinders 410 respectively contain an amylase solution and a substrate solution. A hollow cylinder 420 is slidably connected inside cylinder 0. A sample feeding assembly 500 for adding substrate to cylinder 311 is provided at the top of the support shaft 300. The sample feeding assembly 500 includes an annular cylinder 510 rotatably sleeved with the support shaft 300. The annular cylinder 510 contains a substrate solution. A gooseneck tube 520 is fixedly connected to the outside of the annular cylinder 510, allowing the substrate solution to be transported into cylinder 410. The annular cylinder 510... An open sliding connection is provided with a movable cover 530. A replacement assembly 600 for replacing the cylinder 311 is arranged on one side of the water bath 200. The replacement assembly 600 includes a sleeve 610 fixed to the testing machine 100. Two electric push rods 620 are arranged on the outside of the sleeve 610. An L-shaped plate 700 is fixed on the other side of the water bath 200. A bent pipe 710 is fixed on the L-shaped plate 700. The bent pipe 710 can transport iodine solution into the cylinder 311.

[0021] Specifically, multiple cylinders 311 are rotatably connected inside the detection machine 100. Each cylinder 311 can be transferred to the inclined tube 411 position of two cylinders 410 to hold enzyme and substrate solution containing starch. When multiple cylinders 311 are filled with the mixture of enzyme and substrate, the water bath temperature inside the water bath 200 can be adjusted in a gradient. After each temperature adjustment, it is detected whether the amylase inside one cylinder 311 has completed the hydrolysis of starch, thereby realizing the detection of enzyme activity under multiple gradient temperatures.

[0022] When a fixed amount of enzyme needs to be reacted with the maximum amount of substrate, the substrate solution is pre-filled into the annular cylinder 510. As the support shaft 300 rotates multiple cylinders 311 to the inclined tube 411 to collect the enzyme and substrate mixture, the screw 320 at the top of the support shaft 300 drives the movable cover 530 to move down, transporting the substrate solution inside the annular cylinder 510 through the gooseneck tube 520 to the cylinder 410 used to hold the substrate solution. This results in a greater gradient of substrate solution added to the cylinder 311 than the amount added to the cylinder 311 previously. This facilitates the detection of the maximum reaction limit of the enzyme by gradually increasing the amount of substrate under a fixed enzyme amount. The enzyme activity detection and the maximum reaction limit detection are implemented in a linked manner, improving the overall efficiency of enzyme activity detection.

[0023] like Figure 5 and Figure 6 As shown, in this embodiment, a protrusion 211 is fixed at the top of the support ring 210 and abuts against the bottom surface of the cylinder 311. The protrusion 211 is arc-shaped. Two support plates are fixed between the bottom surface of the support ring 210 and the water bath 200. When the collar 310 rotates the cylinder 311 to the position of the protrusion 211, the protrusion 211 can lift the bottom surface of the cylinder 311 to a position flush with the top surface of the collar 310, so that the replacement component 600 can automatically replace the new cylinder 311 to hold the mixture of enzyme and substrate.

[0024] like Figure 5 As shown, in this embodiment, the outer side of the sleeve 610 is connected to an inclined tube 411. When the first cylinder 311 rotates to the bottom of the two inclined tubes 411, the liquid level inside the second cylinder 410 will submerge the inlet of the inclined tube 411 as the hollow cylinder 420 moves down, so that the two second cylinders 410 can inject a certain amount of amylase solution and substrate solution into the first cylinder 311 through the inclined tube 411.

[0025] like Figure 4 and Figure 5 As shown, in this embodiment, a collection box 220 is fixed to the outside of the water bath 200. The collection box 220 is used to collect the cylinder 311 that has been replaced after use. A water pipe is fixed to the outside of the collection box 220. The water pipe is used to discharge the mixture of enzyme and substrate inside the collection box 220. A heating block 230 is fixed to the inner bottom surface of the water bath 200. A heating wire of the prior art is installed inside the heating block 230. When the heating wire is energized, the heating block 230 is heated, thereby raising the water temperature. A conventional water thermometer is also installed inside the water bath 200 to monitor the water bath heating temperature in real time. Two water pipes are also fixed to the outside of the water bath 200. One water pipe is for water inlet and the other is for water outlet, which facilitates the replacement of the water inside the water bath 200.

[0026] In this embodiment, when studying the effect of temperature on enzyme-catalyzed reactions, two cylinders 410 were preheated to a certain temperature inside a water bath 200 with the enzyme and substrate, and then injected into the same cylinder 311. The enzyme and substrate can react rapidly at the water temperature, and the bottom of cylinder 311 is also immersed in water, which helps the enzyme and substrate to react at the temperature required for the experiment.

[0027] like Figure 3 and Figure 4 As shown, in this embodiment, the infusion assembly 400 also includes an L-shaped frame 430 fixed to the water bath 200. A screw 431 is rotatably connected to the L-shaped frame 430. A crossbeam 421 fixed to the hollow cylinder 420 is screwed to the outside of the screw 431. A motor 110 capable of driving the screw 431 to rotate is fixed to the top of the L-shaped frame 430.

[0028] In this embodiment, when it is necessary to inject the solution (amylase solution or substrate solution) inside the second cylinder 410 into the first cylinder 311, the first motor 110 drives the second screw 431 to rotate. The crossbeam 421, which is screwed to the second screw 431, moves down along the second cylinder 410 with the two hollow cylinders 420, thereby causing the hollow cylinders 420 to press the liquid level of the solution inside the second cylinder 410 to the inlet of the inclined tube 411, so that the solution inside the second cylinder 410 can enter the first cylinder 311 from the inclined tube 411.

[0029] like Figure 4 As shown, in this embodiment, the top surface of the hollow cylinder 420 is detachably fixed to the crossbeam 421 by bolts, which makes it convenient for the user to remove the hollow cylinder 420 from the crossbeam 421 to clean the second cylinder 410, so that the second cylinder 410 can be filled with different substrate solutions. The outer side of the hollow cylinder 420 is provided with a notch 422. The notch 422 not only facilitates the injection of the gradient-added substrate solution into the second cylinder 410 by the gooseneck tube 520, but also facilitates the lifting of the solution level inside the second cylinder 410 from the notch 422 when the hollow cylinder 420 moves down along the second cylinder 410, so that the solution inside the second cylinder 410 can flow into the first cylinder 311 along the inclined tube 411.

[0030] like Figure 2 , Figure 3 and Figure 6As shown, in this embodiment, a connecting plate is fixed between the sleeve 610 and the testing machine 100, thereby fixing the sleeve 610 on the testing machine 100. Multiple cylinders 311 are placed inside the sleeve 610. A motor 650 is fixed at the bottom of the sleeve 610. An eccentric wheel 651 is fixed at the output end of the motor 650. Two electric push rods 620 are fixedly connected to the annular cylinder 510 and the cylinder 410 respectively. Arc plate 630 and arc plate 640 are fixed at the output ends of the two electric push rods 620 respectively. Corrugated suction cup 631 and arc plate 632 are fixed on the arc plate 630. The arcs corresponding to the arc plates 632 and 640 are concentric.

[0031] In this embodiment, initially, the output of motor 650 drives eccentric wheel 651 to abut against spare cylinder 311 inside sleeve 610 at a position away from the wheel center. Eccentric wheel 651 prevents cylinder 311 from falling out of sleeve 610. Then, when a collar 310 rotates with cylinder 311 that has completed the test to a position directly below sleeve 610, cylinder 311 is lifted by protrusion 211 of support ring 210. The output of electric actuator 620 extends, causing arc plate 630 with corrugated suction cup 631 to abut against cylinder. At point 311, the arc-shaped plate 640 restricts the position of the cylinder 311, causing the corrugated suction cup 631 to firmly contact and adsorb the cylinder 311. Then, the output end of the electric push rod 620 retracts, causing the arc-shaped plate 640 to leave the cylinder 311. The electric push rod 620, along with the arc-shaped plate 630, continues to move towards the cylinder 311, pushing the used cylinder 311 to the top of the collection box 220. Then, the corrugated suction cup 631 releases the cylinder 311, allowing the used cylinder 311 to fall and be collected inside the collection box 220.

[0032] In this embodiment, when a new cylinder 311 needs to be installed on the collar 310 below the sleeve 610, the electric actuator 620 drives the arc plate 630 and the arc plate 640 back to their initial positions. Then, the output of the motor 650 drives the eccentric wheel 651 to rotate. The eccentric wheel 651 no longer abuts against the cylinder 311, causing a spare cylinder 311 inside the sleeve 610 to fall to the top of the protrusion 211 of the support ring 210. The arc plate 640 and the corrugated suction cup 631 are arranged on the outside of the cylinder 311, combined with... Figure 7 Because the arc plate 3 640 and the arc plate 2 632 are concentric, the cylinder 1 311 can move stably along the space between the arc plate 2 632 and the arc plate 3 640 towards the inclined tube 411. As the cylinder 1 311 gradually moves away from the protrusion 211, the cylinder 1 311 inside the sleeve 610 moves down, making it easier for the collar 310 to be fitted onto the outside of the cylinder 1 311, thus improving the stability of the cylinder 1 311's subsequent rotational adjustment position.

[0033] like Figure 5 As shown, in this embodiment, a camera 720 is installed on the L-shaped plate 700, and the bent tube 710 is connected and fixed to the pipe for conveying iodine solution. After the starch solution inside the cylinder 311 reacts with the substrate for a period of time, an appropriate amount of iodine solution can be injected into the cylinder 311 through the bent tube 710. The bent tube 710 is connected to the pipe for conveying iodine solution. If a deep blue color is seen through the camera 720, it indicates that the starch in the substrate has not been fully hydrolyzed. If a purple or red color is seen, it indicates that the substrate has been partially hydrolyzed, and the enzyme is active but not completely hydrolyzed. If a colorless or brownish-yellow color is seen, it indicates that the starch in the substrate has been completely hydrolyzed. Users can directly view the image transmitted by the camera 720 on the display screen outside the detector 100 to facilitate the user's assessment of the enzyme activity.

[0034] like Figure 2 and Figure 8 As shown, in this embodiment, a second motor capable of driving the support shaft 300 to rotate is fixed to the inner bottom of the testing machine 100, a first screw 320 is fixed to the top of the support shaft 300, an L-shaped block 531 that is screwed and connected to the first screw 320 is fixed to the top of the movable cover 530, an L-shaped frame 540 is fixed between the annular cylinder 510 and the water bath 200, and a positioning rod 541 that is slidably connected to the L-shaped block 531 is fixed on the L-shaped frame 540.

[0035] In this embodiment, when it is necessary to study the effect of increasing substrate amount on enzyme activity, a spare substrate solution can be pre-injected into the annular cylinder 510 through the gooseneck tube 520. Then, during the process of the support shaft 300 driving multiple collars 310 to rotate 45 degrees, the screw 320 at the top of the support shaft 300 will engage and drive the L-shaped block 531 with the movable cover 530 to move down a certain distance (denoted as a). During the downward movement, the substrate solution inside the annular cylinder 510 can be injected into the cylinder 410 through the gooseneck tube 520. Thus, even if the two hollow cylinders 420 move down to the same depth at one time, the cylinder 410 can be additionally filled with the substrate solution output from the gooseneck tube 520 into the cylinder 311 through the inclined tube 411.

[0036] In the initial state, the rotation of screw 431 causes the crossbeam 421 to move the two hollow cylinders 420 downwards by the same distance, thereby injecting equal amounts of enzyme solution and substrate solution into cylinder 311 through the inclined tube 411 (cylinder 311 that rotates to the bottom of the inclined tube 411 is sequentially labeled as cylinder number one, cylinder number two, cylinder number three, etc.). At this time, cylinder number one contains equal amounts of enzyme solution and substrate solution. Then, the support shaft 300 rotates 45 degrees with the collar 310 to move cylinder number two to the bottom of the inclined tube 411. During this process, the screw 320 rotates 45 degrees synchronously, causing the active enzyme solution inside the annular cylinder 510 to be released. The movable cover 530 is moved down a distance 'a', causing the annular cylinder 510 to inject an additional amount of substrate solution into the substrate cylinder 410 (the amount of substrate solution added in a gradient is denoted as 'b'). Then, the support shaft 300 is rotated 90 degrees, causing the fourth cylinder to rotate below the inclined tube 411. At this time, the amount of substrate added to the substrate cylinder 410 through the annular cylinder 510 is 2b. The two hollow cylinders 420 are moved down the same distance again, so that the amount of substrate in the fourth cylinder can be increased by 'b' compared to the amount of substrate in the second cylinder, and the amount of substrate in the second cylinder can be increased by 'b' compared to the amount of substrate in the first cylinder. This process can be repeated to design multiple sets of substrate-gradient cylinders 311.

[0037] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0038] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A temperature control and micro-volume sampling precision linkage device for enzyme activity detection, comprising a detector (100), characterized in that, Also includes: A water bath tank (200) has a support ring (210) fixed inside it. A support shaft (300) is rotatably connected to a water bath (200). Multiple collars (310) are evenly fixed on the outer side of the support shaft (300). A cylindrical ring (311) that contacts the support ring (210) is sleeved on the collar (310). An infusion assembly (400) includes two cylindrical tubes (410) detachably mounted to a water bath (200), and a hollow tube (420) is slidably connected inside the cylindrical tubes (410). The sample dispensing assembly (500) includes an annular cylinder (510) rotatably sleeved with a support shaft (300), a gooseneck tube (520) is fixedly connected to the outside of the annular cylinder (510), and a movable cover (530) is slidably connected to the opening of the annular cylinder (510). Replacement assembly (600) includes a sleeve (610) fixed to the testing machine (100), and two electric actuators (620) are arranged on the outside of the sleeve. An L-shaped plate (700) is fixed to a water bath tank (200), and a bent pipe (710) is fixed on the L-shaped plate (700).

2. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 1, characterized in that, The top of the support ring (210) is fixed with a protrusion (211) that abuts against the bottom surface of the first cylinder (311), and the outside of the first cylinder (410) is connected to and fixed with an inclined tube (411).

3. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 1, characterized in that, A collection box (220) is fixed to the outside of the water bath (200), and a heating block (230) is fixed to the bottom surface of the water bath (200).

4. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 1, characterized in that, The infusion assembly (400) also includes an L-shaped frame (430) fixed to the water bath (200), a screw (431) is rotatably connected to the L-shaped frame (430), and a crossbeam (421) is screwed to the outside of the screw (431) and fixed to the hollow cylinder (420).

5. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 1, characterized in that, A connecting plate is fixed between the sleeve (610) and the testing machine (100). Multiple cylinders (311) are placed inside the sleeve (610). A motor (650) is fixed at the bottom of the sleeve (610). An eccentric wheel (651) is fixed at the output end of the motor (650).

6. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 1, characterized in that, A camera (720) is installed on the L-shaped plate (700), and the bent pipe (710) is connected and fixed to the pipe for conveying iodine solution.

7. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 1, characterized in that, The bottom of the testing machine (100) is fixed with a second motor that can drive the support shaft (300) to rotate, and the top of the support shaft (300) is fixed with a first screw (320).

8. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 7, characterized in that, The top of the movable cover (530) is fixed with an L-shaped block (531) that is screwed into the screw (320). An L-shaped frame (540) is fixed between the annular cylinder (510) and the water bath (200). A positioning rod (541) that is slidably connected to the L-shaped block (531) is fixed on the L-shaped frame (540).

9. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 8, characterized in that, The two electric actuators (620) are fixedly connected to the annular cylinder (510) and the sleeve (410) respectively, and the output ends of the two electric actuators (620) are respectively fixed with the arc plate (630) and the arc plate (640).

10. The temperature control and micro-volume sampling precision linkage device for enzyme activity detection according to claim 9, characterized in that, A corrugated suction cup (631) and an arc plate two (632) are fixed on the arc plate one (630), and the arc plate two (632) and the arc plate three (640) share the same center.

Images